Lonrf3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lonrf3
Entrez Gene ID	74365
Full Name	LON peptidase N-terminal domain and ring finger 3
Synonyms	4932412G04Rik,5730439E01Rik,A830039N02Rik,AU023707,Rnf127
General protein information	Preferred Names LON peptidase N-terminal domain and ring finger 3 Names LON peptidase N-terminal domain and RING finger protein 3 ring finger protein 127
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X A3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_028894.1	NP_083170.1	LON peptidase N-terminal domain and RING finger protein 3
XM_006541404.1	XP_006541467.1	LON peptidase N-terminal domain and RING finger protein 3 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LONRF3	XP_005641782.1
Mus musculus (house mouse)	Lonrf3	NP_083170.1
Pan troglodytes (chimpanzee)	LONRF3	XP_001135253.1
Rattus norvegicus (Norway rat)	Lonrf3	NP_001178514.1
Xenopus tropicalis (tropical clawed frog)	lonrf3	XP_002934588.2
Bos taurus (cattle)	LONRF3	NP_001178063.1
Gallus gallus (chicken)	LONRF3	XP_420345.3
Homo sapiens (human)	LONRF3	NP_001027026.1
	LONRF3	XP_001105370.2

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Gineste C, De Winter JM, Kohl C, Witt CC, Giannesini B, Brohm K, Le Fur Y, Gretz N, Vilmen C, Pecchi E, Jubeau M, Cozzone PJ, Stienen GJ, Granzier H, Labeit S, Ottenheijm CA, Bendahan D, Gondin J
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lonrf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lonrf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06293	NM_028894.1 Latest version!	Mus musculus LON peptidase N-terminal domain and ring finger 3 (Lonrf3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$440.30 $629.00
OMu55990	XM_006541404.1 Latest version!	Mus musculus LON peptidase N-terminal domain and ring finger 3 (Lonrf3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lonrf3 ( NM_028894.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083170.1 Mus musculus LON peptidase N-terminal domain and ring finger 3 (Lonrf3), mRNA.

Price & Availability↑

CloneID	OMu06293
Clone ID Related Accession (Same CDS sequence)	NM_028894.1
Accession Version	NM_028894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	LON peptidase N-terminal domain and RING finger protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY740851.1, AK016522.1, AL450395.7 and BC058671.1. On Jul 22, 2005 this sequence version replaced XM_135829.1. ##Evidence-Data-START## Transcript exon combination :: AK167341.1, AK016522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGATTCCC TTCAGACCGC ACAGATGGTG AGCTTGTCCG CCGAGCTCGG CAGCAACAAC 
TTGGAGCTGG CGGAGCCGGA GGAGCCGGGG ACATCAGCGG CCGCAGGGCA GTCAGCCGCC 
CACCCGGAGG AGGTGACACC CGAGGGCTCC CAAGCTCTCG GGGCTCAAGA GCCCGAGCAA 
AGCCTGCCTC TGGCGGTCCC GACACCGCTG GAGTGCAAAG TCCTGCTCAC GCAGGCGGAT 
GCCTTGGCAT CTGAGGGGCA CCTTCGGGAA GCCCTCGAGG TGTACCGACA GCTCTCCGAG 
AGGCAGCAGC TCGTAGCGGA GCAGCTGGAG CAGCTAGTGC GCTGCTTGGC CGACAGTGTC 
CCGCAAGAGG AGCTGGCCTC CGACTCGAGC GGCACTTCGA GCTGCTGCGC CGCGGCGCTG 
AAAGAGGCAG GGGAGGCCGC GGCCGTGGCC CCCGAGGTAT GGGATGGCTT TAAGTGCAAG 
AAGTGTCACG GGTTTCTCTC AGACCCGGTG TCCCTGTGGT GCGGCCACAC CTTTTGTAAA 
CTGTGCCTGG AGCGCGGGCG AGCCGCCGAT CGGCGCTGCG CTCTGTGCGG GGTCAAGCTC 
TCCGCTCTGA TGGCGGCCAG TGGGAGGGCA CGCGGGCCGC GGCGCGCGGG GCAGCCGGCG 
CCCCTGCAAC TGCGAGTTAA CGTGGTACTC AGCGGCCTCC TGGGCAAGTT GTTCCCGGGC 
CCCGCGCGGG CGTCGCAACT CCGGCACGAG GGCAACCGGC TGTTCCGCGA GCACCAGGTG 
GAGGCAGCTC TGCTCAAGTA CAACGAGGCT GTTCGTCTAG CTCCAAACGA CCATTTGCTT 
TATAGCAATC GGTCCCAAAT TTATTTCACC TTGGAGTCTC ATGAGGATGC ACTGCATGAT 
GCAGAAATAG CATGTAAACT ACGGCCAATG GGTTTTAAGG CACACTTCAG AAAAGCACAA 
GCTTTAGCCA CTCTAGGCAA GGTGAAAGAA GCACTCAAAG AATTTCTCTA CTGCGTATCC 
CTTGATGGAA AGAACAAGAG CGCCAGATCT GAAGCTCAAA GGGAGAATCT GGAGCTTCCC 
CATTGTTCTA ATCAGGAGGG AGCAGCAGCT GCAGAAGAGA GCAGCAGCCT AGCAAATTCC 
GCTCAAGGAA AGGTGAGTAG TAAAGAAGAC AGAAAGAAAG ATCAGGAAGG AGAGGACCGG 
GATGCTGCCT CCGTCAGGAC CGGCAAATGC CAGGAAAAGA AGAGAAATCG TTGCCAAATC 
GAAACCCAAG AAGACACAGA ATTGCCTAAT AAAGTCTCCA AACAAGATTT TCCTGCTGAG 
CAGGGAGCCA AACCAGATCT CAGTAATCCA CTTGGATCCT TTGATGCATC TGACCTTGAA 
TGCTCACTGT GTATGAGATT ATTCTATGAG CCAGTCACAA CACCTTGTGG ACACACCTTT 
TGCTTGAAAT GTCTTGAGAG ATGCCTAGAT CATAATGCAA AGTGTCCACT GTGCAAAGAC 
GTTCTTTTAC AGTGCTTGCC ATCAAGAAAA TATAGCAAAA ATGTAATCCT GGAAGAACTC 
ATAGCTACGT TCCTGCCAGA AGAATTCAAG GAACGAAAGA GGCTTTATGA AGAGGAAATG 
GAAGAGCTTT CTAACTTAAA TAAGAATGTC CCTATTTTCG TGTGCACCAT GGCCTATCCC 
ACCGTTCCTT GTCCCTTGCA CATCTTTGAG CCTTGTTACC GCCTGATGAT TCGGAGATGT 
ATTGAGACAG GCACAAGACA GTTTGGCATG TGCCTTGGAG ATCCTGTCAA AGGGTTTGTA 
GAATATGGCT GCATCTTAGA GATCAGAAAT GTCCAATTCT TTTCTGATGG CCGATCGGTG 
GTAGACAGCA TAGGCAAGAG GCGCTTCAAA GTGCTCCATC AGGGTCAACG GGATGGCTAC 
AACACTGCTG ACATTGAATA CATTGAAGAT CAAAAGGTTC AGGGAGATGA TTGTGCTGAA 
CTTATGGGGT TACACAACTG TGTGTATGAG CAAGCGTCAT CATGGTTTCA CTCGCTCAAA 
GCATCTCTGA AGAATCGGAT ACTAAATCAC TTTGGTCCAA TGCCAGAGAA GGACGAAGAC 
CCCCAGGTTA ATCCGAATGG TCCAGCCTGG TGCTGGTGGA CACTGGCGGT TCTTCCTCTG 
GAAAGCAGAG CTCAGCTTCC CTTCCTAGCA ATGAGGTCCT TAAAGGACAG ATTGAATGGT 
ATTCGGCGTA TCCTGGCCTT TATATCGAGA AACCAAAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083170.1
CDS	172..2433
Translation	MDSLQTAQMVSLSAELGSNNLELAEPEEPGTSAAAGQSAAHPEEVTPEGSQALGAQEPEQSLPLAVPTPLECKVLLTQADALASEGHLREALEVYRQLSERQQLVAEQLEQLVRCLADSVPQEELASDSSGTSSCCAAALKEAGEAAAVAPEVWDGFKCKKCHGFLSDPVSLWCGHTFCKLCLERGRAADRRCALCGVKLSALMAASGRARGPRRAGQPAPLQLRVNVVLSGLLGKLFPGPARASQLRHEGNRLFREHQVEAALLKYNEAVRLAPNDHLLYSNRSQIYFTLESHEDALHDAEIACKLRPMGFKAHFRKAQALATLGKVKEALKEFLYCVSLDGKNKSARSEAQRENLELPHCSNQEGAAAAEESSSLANSAQGKVSSKEDRKKDQEGEDRDAASVRTGKCQEKKRNRCQIETQEDTELPNKVSKQDFPAEQGAKPDLSNPLGSFDASDLECSLCMRLFYEPVTTPCGHTFCLKCLERCLDHNAKCPLCKDVLLQCLPSRKYSKNVILEELIATFLPEEFKERKRLYEEEMEELSNLNKNVPIFVCTMAYPTVPCPLHIFEPCYRLMIRRCIETGTRQFGMCLGDPVKGFVEYGCILEIRNVQFFSDGRSVVDSIGKRRFKVLHQGQRDGYNTADIEYIEDQKVQGDDCAELMGLHNCVYEQASSWFHSLKASLKNRILNHFGPMPEKDEDPQVNPNGPAWCWWTLAVLPLESRAQLPFLAMRSLKDRLNGIRRILAFISRNQN

Target ORF information:

RefSeq Version	NM_028894.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LON peptidase N-terminal domain and ring finger 3 (Lonrf3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028894.1

ORF Insert Sequence:

ATGGATTCCC TTCAGACCGC ACAGATGGTG AGCTTGTCCG CCGAGCTCGG CAGCAACAAC 
TTGGAGCTGG CGGAGCCGGA GGAGCCGGGG ACATCAGCGG CCGCAGGGCA GTCAGCCGCC 
CACCCGGAGG AGGTGACACC CGAGGGCTCC CAAGCTCTCG GGGCTCAAGA GCCCGAGCAA 
AGCCTGCCTC TGGCGGTCCC GACACCGCTG GAGTGCAAAG TCCTGCTCAC GCAGGCGGAT 
GCCTTGGCAT CTGAGGGGCA CCTTCGGGAA GCCCTCGAGG TGTACCGACA GCTCTCCGAG 
AGGCAGCAGC TCGTAGCGGA GCAGCTGGAG CAGCTAGTGC GCTGCTTGGC CGACAGTGTC 
CCGCAAGAGG AGCTGGCCTC CGACTCGAGC GGCACTTCGA GCTGCTGCGC CGCGGCGCTG 
AAAGAGGCAG GGGAGGCCGC GGCCGTGGCC CCCGAGGTAT GGGATGGCTT TAAGTGCAAG 
AAGTGTCACG GGTTTCTCTC AGACCCGGTG TCCCTGTGGT GCGGCCACAC CTTTTGTAAA 
CTGTGCCTGG AGCGCGGGCG AGCCGCCGAT CGGCGCTGCG CTCTGTGCGG GGTCAAGCTC 
TCCGCTCTGA TGGCGGCCAG TGGGAGGGCA CGCGGGCCGC GGCGCGCGGG GCAGCCGGCG 
CCCCTGCAAC TGCGAGTTAA CGTGGTACTC AGCGGCCTCC TGGGCAAGTT GTTCCCGGGC 
CCCGCGCGGG CGTCGCAACT CCGGCACGAG GGCAACCGGC TGTTCCGCGA GCACCAGGTG 
GAGGCAGCTC TGCTCAAGTA CAACGAGGCT GTTCGTCTAG CTCCAAACGA CCATTTGCTT 
TATAGCAATC GGTCCCAAAT TTATTTCACC TTGGAGTCTC ATGAGGATGC ACTGCATGAT 
GCAGAAATAG CATGTAAACT ACGGCCAATG GGTTTTAAGG CACACTTCAG AAAAGCACAA 
GCTTTAGCCA CTCTAGGCAA GGTGAAAGAA GCACTCAAAG AATTTCTCTA CTGCGTATCC 
CTTGATGGAA AGAACAAGAG CGCCAGATCT GAAGCTCAAA GGGAGAATCT GGAGCTTCCC 
CATTGTTCTA ATCAGGAGGG AGCAGCAGCT GCAGAAGAGA GCAGCAGCCT AGCAAATTCC 
GCTCAAGGAA AGGTGAGTAG TAAAGAAGAC AGAAAGAAAG ATCAGGAAGG AGAGGACCGG 
GATGCTGCCT CCGTCAGGAC CGGCAAATGC CAGGAAAAGA AGAGAAATCG TTGCCAAATC 
GAAACCCAAG AAGACACAGA ATTGCCTAAT AAAGTCTCCA AACAAGATTT TCCTGCTGAG 
CAGGGAGCCA AACCAGATCT CAGTAATCCA CTTGGATCCT TTGATGCATC TGACCTTGAA 
TGCTCACTGT GTATGAGATT ATTCTATGAG CCAGTCACAA CACCTTGTGG ACACACCTTT 
TGCTTGAAAT GTCTTGAGAG ATGCCTAGAT CATAATGCAA AGTGTCCACT GTGCAAAGAC 
GTTCTTTTAC AGTGCTTGCC ATCAAGAAAA TATAGCAAAA ATGTAATCCT GGAAGAACTC 
ATAGCTACGT TCCTGCCAGA AGAATTCAAG GAACGAAAGA GGCTTTATGA AGAGGAAATG 
GAAGAGCTTT CTAACTTAAA TAAGAATGTC CCTATTTTCG TGTGCACCAT GGCCTATCCC 
ACCGTTCCTT GTCCCTTGCA CATCTTTGAG CCTTGTTACC GCCTGATGAT TCGGAGATGT 
ATTGAGACAG GCACAAGACA GTTTGGCATG TGCCTTGGAG ATCCTGTCAA AGGGTTTGTA 
GAATATGGCT GCATCTTAGA GATCAGAAAT GTCCAATTCT TTTCTGATGG CCGATCGGTG 
GTAGACAGCA TAGGCAAGAG GCGCTTCAAA GTGCTCCATC AGGGTCAACG GGATGGCTAC 
AACACTGCTG ACATTGAATA CATTGAAGAT CAAAAGGTTC AGGGAGATGA TTGTGCTGAA 
CTTATGGGGT TACACAACTG TGTGTATGAG CAAGCGTCAT CATGGTTTCA CTCGCTCAAA 
GCATCTCTGA AGAATCGGAT ACTAAATCAC TTTGGTCCAA TGCCAGAGAA GGACGAAGAC 
CCCCAGGTTA ATCCGAATGG TCCAGCCTGG TGCTGGTGGA CACTGGCGGT TCTTCCTCTG 
GAAAGCAGAG CTCAGCTTCC CTTCCTAGCA ATGAGGTCCT TAAAGGACAG ATTGAATGGT 
ATTCGGCGTA TCCTGGCCTT TATATCGAGA AACCAAAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lonrf3 ( XM_006541404.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541467.1 Mus musculus LON peptidase N-terminal domain and ring finger 3 (Lonrf3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55990
Clone ID Related Accession (Same CDS sequence)	XM_006541404.1
Accession Version	XM_006541404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	LON peptidase N-terminal domain and RING finger protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCC TTCAGACCGC ACAGATGGTG AGCTTGTCCG CCGAGCTCGG CAGCAACAAC 
TTGGAGCTGG CGGAGCCGGA GGAGCCGGGG ACATCAGCGG CCGCAGGGCA GTCAGCCGCC 
CACCCGGAGG AGGTGACACC CGAGGGCTCC CAAGCTCTCG GGGCTCAAGA GCCCGAGCAA 
AGCCTGCCTC TGGCGGTCCC GACACCGCTG GAGTGCAAAG TCCTGCTCAC GCAGGCGGAT 
GCCTTGGCAT CTGAGGGGCA CCTTCGGGAA GCCCTCGAGG TGTACCGACA GCTCTCCGAG 
AGGCAGCAGC TCGTAGCGGA GCAGCTGGAG CAGCTAGTGC GCTGCTTGGC CGACAGTGTC 
CCGCAAGAGG AGCTGGCCTC CGACTCGAGC GGCACTTCGA GCTGCTGCGC CGCGGCGCTG 
AAAGAGGCAG GGGAGGCCGC GGCCGTGGCC CCCGAGGTAT GGGATGGCTT TAAGTGCAAG 
AAGTGTCACG GGTTTCTCTC AGACCCGGTG TCCCTGTGGT GCGGCCACAC CTTTTGTAAA 
CTGTGCCTGG AGCGCGGGCG AGCCGCCGAT CGGCGCTGCG CTCTGTGCGG GGTCAAGCTC 
TCCGCTCTGA TGGCGGCCAG TGGGAGGGCA CGCGGGCCGC GGCGCGCGGG GCAGCCGGCG 
CCCCTGCAAC TGCGAGTTAA CGTGGTACTC AGCGGCCTCC TGGGCAAGTT GTTCCCGGGC 
CCCGCGCGGG CGTCGCAACT CCGGCACGAG GGCAACCGGC TGTTCCGCGA GCACCAGGTG 
GAGGCAGCTC TGCTCAAGTA CAACGAGGCT GTTCGTCTAG CTCCAAACGA CCATTTGCTT 
TATAGCAATC GGTCCCAAAT TTATTTCACC TTGGAGTCTC ATGAGGATGC ACTGCATGAT 
GCAGAAATAG CATGTAAACT ACGGCCAATG GGTTTTAAGG CACACTTCAG AAAAGCACAA 
GCTTTAGCCA CTCTAGGCAA GGTGAAAGAA GCACTCAAAG AATTTCTCTA CTGCGTATCC 
CTTGATGGAA AGAACAAGAG CGCCAGATCT GAAGCTCAAA GGCTTCTCTT TAGTTTCTTT 
TCACCATCAG TCCCGGGGGA GTCTCAGGAA CATTCTCCCG ATATCCTGAA GCTGTTGGCA 
CCTCACCCTA GATTAAAGGA ACACGTAGAG TCAATGGCTA CTGAAGGCAC CAGCCATAAT 
CTACCAAAGT TGTCACAGGA GAATCTGGAG CTTCCCCATT GTTCTAATCA GGAGGGAGCA 
GCAGCTGCAG AAGAGAGCAG CAGCCTAGCA AATTCCGCTC AAGGAAAGGT GAGTAGTAAA 
GAAGACAGAA AGAAAGATCA GGAAGGAGAG GACCGGGATG CTGCCTCCGT CAGGACCGGC 
AAATGCCAGG AAAAGAAGAG AAATCGTTGC CAAATCGAAA CCCAAGAAGA CACAGAATTG 
CCTAATAAAG TCTCCAAACA AGATTTTCCT GCTGAGCAGG GAGCCAAACC AGATCTCAGT 
AATCCACTTG GATCCTTTGA TGCATCTGAC CTTGAATGCT CACTGTGTAT GAGATTATTC 
TATGAGCCAG TCACAACACC TTGTGGACAC ACCTTTTGCT TGAAATGTCT TGAGAGATGC 
CTAGATCATA ATGCAAAGTG TCCACTGTGC AAAGACGTTC TTTTACAGTG CTTGCCATCA 
AGAAAATATA GCAAAAATGT AATCCTGGAA GAACTCATAG CTACGTTCCT GCCAGAAGAA 
TTCAAGGAAC GAAAGAGGCT TTATGAAGAG GAAATGGAAG AGCTTTCTAA CTTAAATAAG 
AATGTCCCTA TTTTCGTGTG CACCATGGCC TATCCCACCG TTCCTTGTCC CTTGCACATC 
TTTGAGCCTT GTTACCGCCT GATGATTCGG AGATGTATTG AGACAGGCAC AAGACAGTTT 
GGCATGTGCC TTGGAGATCC TGTCAAAGGG TTTGTAGAAT ATGGCTGCAT CTTAGAGATC 
AGAAATGTCC AATTCTTTTC TGATGGCCGA TCGGTGGTAG ACAGCATAGG CAAGAGGCGC 
TTCAAAGTGC TCCATCAGGG TCAACGGGAT GGCTACAACA CTGCTGACAT TGAATACATT 
GAAGATCAAA AGGTTCAGGG AGATGATTGT GCTGAACTTA TGGGGTTACA CAACTGTGTG 
TATGAGCAAG CGTCATCATG GTTTCACTCG CTCAAAGCAT CTCTGAAGAA TCGGATACTA 
AATCACTTTG GTCCAATGCC AGAGAAGGAC GAAGACCCCC AGGTTAATCC GAATGGTCCA 
GCCTGGTGCT GGTGGACACT GGCGGTTCTT CCTCTGGAAA GCAGAGCTCA GCTTCCCTTC 
CTAGCAATGA GGTCCTTAAA GGACAGATTG AATGGTATTC GGCGTATCCT GGCCTTTATA 
TCGAGAAACC AAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541467.1
CDS	223..2640
Translation	MDSLQTAQMVSLSAELGSNNLELAEPEEPGTSAAAGQSAAHPEEVTPEGSQALGAQEPEQSLPLAVPTPLECKVLLTQADALASEGHLREALEVYRQLSERQQLVAEQLEQLVRCLADSVPQEELASDSSGTSSCCAAALKEAGEAAAVAPEVWDGFKCKKCHGFLSDPVSLWCGHTFCKLCLERGRAADRRCALCGVKLSALMAASGRARGPRRAGQPAPLQLRVNVVLSGLLGKLFPGPARASQLRHEGNRLFREHQVEAALLKYNEAVRLAPNDHLLYSNRSQIYFTLESHEDALHDAEIACKLRPMGFKAHFRKAQALATLGKVKEALKEFLYCVSLDGKNKSARSEAQRLLFSFFSPSVPGESQEHSPDILKLLAPHPRLKEHVESMATEGTSHNLPKLSQENLELPHCSNQEGAAAAEESSSLANSAQGKVSSKEDRKKDQEGEDRDAASVRTGKCQEKKRNRCQIETQEDTELPNKVSKQDFPAEQGAKPDLSNPLGSFDASDLECSLCMRLFYEPVTTPCGHTFCLKCLERCLDHNAKCPLCKDVLLQCLPSRKYSKNVILEELIATFLPEEFKERKRLYEEEMEELSNLNKNVPIFVCTMAYPTVPCPLHIFEPCYRLMIRRCIETGTRQFGMCLGDPVKGFVEYGCILEIRNVQFFSDGRSVVDSIGKRRFKVLHQGQRDGYNTADIEYIEDQKVQGDDCAELMGLHNCVYEQASSWFHSLKASLKNRILNHFGPMPEKDEDPQVNPNGPAWCWWTLAVLPLESRAQLPFLAMRSLKDRLNGIRRILAFISRNQN

Target ORF information:

RefSeq Version	XM_006541404.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LON peptidase N-terminal domain and ring finger 3 (Lonrf3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541404.1

ORF Insert Sequence:

ATGGATTCCC TTCAGACCGC ACAGATGGTG AGCTTGTCCG CCGAGCTCGG CAGCAACAAC 
TTGGAGCTGG CGGAGCCGGA GGAGCCGGGG ACATCAGCGG CCGCAGGGCA GTCAGCCGCC 
CACCCGGAGG AGGTGACACC CGAGGGCTCC CAAGCTCTCG GGGCTCAAGA GCCCGAGCAA 
AGCCTGCCTC TGGCGGTCCC GACACCGCTG GAGTGCAAAG TCCTGCTCAC GCAGGCGGAT 
GCCTTGGCAT CTGAGGGGCA CCTTCGGGAA GCCCTCGAGG TGTACCGACA GCTCTCCGAG 
AGGCAGCAGC TCGTAGCGGA GCAGCTGGAG CAGCTAGTGC GCTGCTTGGC CGACAGTGTC 
CCGCAAGAGG AGCTGGCCTC CGACTCGAGC GGCACTTCGA GCTGCTGCGC CGCGGCGCTG 
AAAGAGGCAG GGGAGGCCGC GGCCGTGGCC CCCGAGGTAT GGGATGGCTT TAAGTGCAAG 
AAGTGTCACG GGTTTCTCTC AGACCCGGTG TCCCTGTGGT GCGGCCACAC CTTTTGTAAA 
CTGTGCCTGG AGCGCGGGCG AGCCGCCGAT CGGCGCTGCG CTCTGTGCGG GGTCAAGCTC 
TCCGCTCTGA TGGCGGCCAG TGGGAGGGCA CGCGGGCCGC GGCGCGCGGG GCAGCCGGCG 
CCCCTGCAAC TGCGAGTTAA CGTGGTACTC AGCGGCCTCC TGGGCAAGTT GTTCCCGGGC 
CCCGCGCGGG CGTCGCAACT CCGGCACGAG GGCAACCGGC TGTTCCGCGA GCACCAGGTG 
GAGGCAGCTC TGCTCAAGTA CAACGAGGCT GTTCGTCTAG CTCCAAACGA CCATTTGCTT 
TATAGCAATC GGTCCCAAAT TTATTTCACC TTGGAGTCTC ATGAGGATGC ACTGCATGAT 
GCAGAAATAG CATGTAAACT ACGGCCAATG GGTTTTAAGG CACACTTCAG AAAAGCACAA 
GCTTTAGCCA CTCTAGGCAA GGTGAAAGAA GCACTCAAAG AATTTCTCTA CTGCGTATCC 
CTTGATGGAA AGAACAAGAG CGCCAGATCT GAAGCTCAAA GGCTTCTCTT TAGTTTCTTT 
TCACCATCAG TCCCGGGGGA GTCTCAGGAA CATTCTCCCG ATATCCTGAA GCTGTTGGCA 
CCTCACCCTA GATTAAAGGA ACACGTAGAG TCAATGGCTA CTGAAGGCAC CAGCCATAAT 
CTACCAAAGT TGTCACAGGA GAATCTGGAG CTTCCCCATT GTTCTAATCA GGAGGGAGCA 
GCAGCTGCAG AAGAGAGCAG CAGCCTAGCA AATTCCGCTC AAGGAAAGGT GAGTAGTAAA 
GAAGACAGAA AGAAAGATCA GGAAGGAGAG GACCGGGATG CTGCCTCCGT CAGGACCGGC 
AAATGCCAGG AAAAGAAGAG AAATCGTTGC CAAATCGAAA CCCAAGAAGA CACAGAATTG 
CCTAATAAAG TCTCCAAACA AGATTTTCCT GCTGAGCAGG GAGCCAAACC AGATCTCAGT 
AATCCACTTG GATCCTTTGA TGCATCTGAC CTTGAATGCT CACTGTGTAT GAGATTATTC 
TATGAGCCAG TCACAACACC TTGTGGACAC ACCTTTTGCT TGAAATGTCT TGAGAGATGC 
CTAGATCATA ATGCAAAGTG TCCACTGTGC AAAGACGTTC TTTTACAGTG CTTGCCATCA 
AGAAAATATA GCAAAAATGT AATCCTGGAA GAACTCATAG CTACGTTCCT GCCAGAAGAA 
TTCAAGGAAC GAAAGAGGCT TTATGAAGAG GAAATGGAAG AGCTTTCTAA CTTAAATAAG 
AATGTCCCTA TTTTCGTGTG CACCATGGCC TATCCCACCG TTCCTTGTCC CTTGCACATC 
TTTGAGCCTT GTTACCGCCT GATGATTCGG AGATGTATTG AGACAGGCAC AAGACAGTTT 
GGCATGTGCC TTGGAGATCC TGTCAAAGGG TTTGTAGAAT ATGGCTGCAT CTTAGAGATC 
AGAAATGTCC AATTCTTTTC TGATGGCCGA TCGGTGGTAG ACAGCATAGG CAAGAGGCGC 
TTCAAAGTGC TCCATCAGGG TCAACGGGAT GGCTACAACA CTGCTGACAT TGAATACATT 
GAAGATCAAA AGGTTCAGGG AGATGATTGT GCTGAACTTA TGGGGTTACA CAACTGTGTG 
TATGAGCAAG CGTCATCATG GTTTCACTCG CTCAAAGCAT CTCTGAAGAA TCGGATACTA 
AATCACTTTG GTCCAATGCC AGAGAAGGAC GAAGACCCCC AGGTTAATCC GAATGGTCCA 
GCCTGGTGCT GGTGGACACT GGCGGTTCTT CCTCTGGAAA GCAGAGCTCA GCTTCCCTTC 
CTAGCAATGA GGTCCTTAAA GGACAGATTG AATGGTATTC GGCGTATCCT GGCCTTTATA 
TCGAGAAACC AAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)
Gineste C,De Winter JM,Kohl C,Witt CC,Giannesini B,Brohm K,Le Fur Y,Gretz N,Vilmen C,Pecchi E,Jubeau M,Cozzone PJ,Stienen GJ,Granzier H,Labeit S,Ottenheijm CA,Bendahan D,Gondin J

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Lonrf3 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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