The following Kcmf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcmf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu83458 | |
| Clone ID Related Accession (Same CDS sequence) | XM_030255640.1 , NM_001347231.1 | |
| Accession Version | NM_001347231.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-25 | |
| Organism | Mus musculus(house mouse) | |
| Product | E3 ubiquitin-protein ligase KCMF1 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153613.9. On Nov 18, 2016 this sequence version replaced XM_017321793.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK007070.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGCAGTGCA TATTAACAAG GGTAGACTTT GATTTGTACT ATGGTGGGGA AGCTTTCTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001334160.1 |
| CDS | 451..1443 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001347231.1 |
1 | ATGCAGTGCA TATTAACAAG GGTAGACTTT GATTTGTACT ATGGTGGGGA AGCTTTCTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu11509 | |
| Clone ID Related Accession (Same CDS sequence) | NM_019715.2 | |
| Accession Version | NM_019715.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Mus musculus(house mouse) | |
| Product | E3 ubiquitin-protein ligase KCMF1 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153613.9, AK142436.1 and CX734813.1. On or before Oct 23, 2008 this sequence version replaced XM_001477544.1, NM_019715.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660819.67876.1, SRR1660811.148328.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGTCCCGAC ATGAAGGTGT CAGCTGTGAT GCATGTTTAA AAGGAAATTT TCGAGGTCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_062689.2 |
| CDS | 351..1496 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_019715.2 |
1 | ATGTCCCGAC ATGAAGGTGT CAGCTGTGAT GCATGTTTAA AAGGAAATTT TCGAGGTCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu83458 | |
| Clone ID Related Accession (Same CDS sequence) | XM_030255640.1 , NM_001347231.1 | |
| Accession Version | XM_030255640.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | E3 ubiquitin-protein ligase KCMF1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCAGTGCA TATTAACAAG GGTAGACTTT GATTTGTACT ATGGTGGGGA AGCTTTCTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_030111500.1 |
| CDS | 4572..5564 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_030255640.1 |
1 | ATGCAGTGCA TATTAACAAG GGTAGACTTT GATTTGTACT ATGGTGGGGA AGCTTTCTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
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Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. |
Cerebellar development transcriptome database (CDT-DB): profiling of spatio-temporal gene expression during the postnatal development of mouse cerebellum. |
Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette. |
Tagging genes with cassette-exchange sites. |
