gRNAs
The following Top1mt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top1mt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu18887 | |
| Clone ID Related Accession (Same CDS sequence) | NM_028404.2 , NM_028404.3 | |
| Accession Version | NM_028404.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-04-28 | |
| Organism | Mus musculus(House mouse) | |
| Product | DNA topoisomerase I, mitochondrial | |
| Comment | PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF362952.1. On Apr 5, 2007 this sequence version replaced NM_028404.1. ##Evidence-Data-START## Transcript exon combination :: AF362952.1, AK147119.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## | |
1 | ATGCTGCTGC TGTGGCTCAG AGCCCTGTGC CGGCGTTTCC AGCATGTCCC CTGCCGCGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_082680.1 |
| CDS | 35..1816 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_028404.2 |
1 | ATGCTGCTGC TGTGGCTCAG AGCCCTGTGC CGGCGTTTCC AGCATGTCCC CTGCCGCGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu18887 | |
| Clone ID Related Accession (Same CDS sequence) | NM_028404.2 , NM_028404.3 | |
| Accession Version | NM_028404.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-17 | |
| Organism | Mus musculus(house mouse) | |
| Product | DNA topoisomerase I, mitochondrial isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116393.10. On Sep 21, 2017 this sequence version replaced NM_028404.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF362952.1, AK147119.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## | |
1 | ATGCTGCTGC TGTGGCTCAG AGCCCTGTGC CGGCGTTTCC AGCATGTCCC CTGCCGCGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_082680.1 |
| CDS | 45..1826 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_028404.3 |
1 | ATGCTGCTGC TGTGGCTCAG AGCCCTGTGC CGGCGTTTCC AGCATGTCCC CTGCCGCGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu51632 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006521466.2 , NM_001355589.1 | |
| Accession Version | NM_001355589.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-17 | |
| Organism | Mus musculus(house mouse) | |
| Product | DNA topoisomerase I, mitochondrial isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116393.10. On Sep 21, 2017 this sequence version replaced XM_006521466.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC145336.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## | |
1 | ATGCTGCTGC TGTGGCTCAG AGCCCTGTGC CGGCGTTTCC AGCATGTCCC CTGCCGCGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001342518.1 |
| CDS | 45..1823 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001355589.1 |
1 | ATGCTGCTGC TGTGGCTCAG AGCCCTGTGC CGGCGTTTCC AGCATGTCCC CTGCCGCGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu51632 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006521466.2 , NM_001355589.1 | |
| Accession Version | XM_006521466.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(House mouse) | |
| Product | DNA topoisomerase I, mitochondrial isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGCTGC TGTGGCTCAG AGCCCTGTGC CGGCGTTTCC AGCATGTCCC CTGCCGCGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006521529.1 |
| CDS | 64..1842 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006521466.2 |
1 | ATGCTGCTGC TGTGGCTCAG AGCCCTGTGC CGGCGTTTCC AGCATGTCCC CTGCCGCGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Transcription profiling suggests that mitochondrial topoisomerase IB acts as a topological barrier and regulator of mitochondrial DNA transcription. |
Lack of mitochondrial topoisomerase I (TOP1mt) impairs liver regeneration. |
Increased negative supercoiling of mtDNA in TOP1mt knockout mice and presence of topoisomerases II? and II? in vertebrate mitochondria. |
Negative regulation of mitochondrial transcription by mitochondrial topoisomerase I. |
