TOP1MT cDNA ORF clone, Homo sapiens(human)

The following TOP1MT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP1MT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu10035 NM_052963.3
Latest version!
Homo sapiens DNA topoisomerase I mitochondrial (TOP1MT), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$249.50-$349.30
$499.00
OHu09875 NM_001258446.1
Latest version!
Homo sapiens DNA topoisomerase I mitochondrial (TOP1MT), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu09875 NM_001258447.1
Latest version!
Homo sapiens DNA topoisomerase I mitochondrial (TOP1MT), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu42348 XM_005250781.4
Latest version!
Homo sapiens DNA topoisomerase I mitochondrial (TOP1MT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
View Old Accession Versions >>
OHu10035 NM_052963.2 Homo sapiens DNA topoisomerase I mitochondrial (TOP1MT), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$249.50-$349.30
$499.00
OHu42348 XM_005250781.3 Homo sapiens topoisomerase (DNA) I, mitochondrial (TOP1MT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu10035
    Clone ID Related Accession (Same CDS sequence) NM_052963.2 , NM_052963.3
    Accession Version NM_052963.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product DNA topoisomerase I, mitochondrial isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC375680.1, CB149603.1, AF349017.1 and AW248607.1. On May 5, 2012 this sequence version replaced NM_052963.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.60669.1, SRR1660807.80.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGCGTGG TGCGGCTGCT GCGGCTCCGG GCGGCTCTGA CGCTGCTCGG GGAGGTCCCC 
    CGCCGCCCGG CCTCCCGGGG TGTCCCGGGC TCGCGCAGGA CGCAGAAGGG CAGTGGAGCC
    AGGTGGGAGA AGGAGAAGCA CGAAGACGGG GTGAAGTGGA GACAGCTGGA GCACAAGGGC
    CCGTACTTCG CACCCCCATA CGAGCCCCTT CCCGACGGAG TGCGTTTCTT CTATGAAGGA
    AGGCCTGTGA GATTGAGCGT GGCAGCGGAG GAGGTCGCCA CTTTTTATGG GAGGATGTTA
    GATCATGAAT ACACAACAAA GGAGGTTTTC CGGAAGAACT TCTTCAATGA CTGGCGAAAG
    GAAATGGCGG TGGAAGAGAG GGAAGTCATC AAGAGCCTGG ACAAGTGTGA CTTCACGGAG
    ATCCACAGAT ACTTTGTGGA CAAGGCCGCA GCCCGGAAAG TCCTGAGCAG GGAGGAGAAG
    CAGAAGCTAA AAGAAGAGGC AGAAAAACTT CAGCAAGAGT TCGGCTACTG TATTTTAGAT
    GGTCACCAAG AAAAAATAGG CAACTTCAAG ATTGAGCCGC CTGGCTTGTT CCGTGGCCGT
    GGCGACCATC CCAAGATGGG GATGCTGAAG AGAAGGATCA CGCCAGAGGA TGTGGTTATC
    AACTGCAGCA GGGACTCGAA GATCCCCGAG CCGCCGGCGG GGCACCAGTG GAAGGAGGTG
    CGCTCCGATA ACACCGTCAC GTGGCTGGCA GCTTGGACCG AGAGCGTTCA GAACTCCATC
    AAGTACATCA TGCTGAACCC TTGCTCGAAG CTGAAGGGGG AGACAGCTTG GCAGAAGTTT
    GAAACAGCTC GACGCCTGCG GGGATTTGTG GACGAGATCC GCTCCCAGTA CCGGGCTGAC
    TGGAAGTCTC GGGAAATGAA GACGAGACAG CGGGCGGTGG CCCTGTATTT CATCGATAAG
    CTGGCACTGA GAGCAGGAAA TGAGAAGGAG GACGGTGAGG CGGCCGACAC CGTGGGCTGC
    TGTTCCCTCC GCGTGGAGCA CGTCCAGCTG CACCCGGAGG CCGATGGCTG CCAACACGTG
    GTGGAATTTG ACTTCCTGGG GAAGGACTGC ATCCGCTACT ACAACAGAGT GCCGGTGGAG
    AAGCCGGTGT ACAAGAACTT ACAGCTCTTT ATGGAGAACA AGGACCCCCG GGACGACCTC
    TTCGACAGGC TGACCACGAC CAGCCTGAAC AAGCACCTCC AGGAGCTGAT GGACGGGCTG
    ACGGCCAAGG TGTTCCGGAC CTACAACGCC TCCATCACTC TGCAGGAGCA GCTGCGGGCC
    CTGACGCGCG CCGAGGACAG CATAGCAGCT AAGATCTTAT CCTACAACCG AGCCAACCGA
    GTCGTGGCCA TTCTCTGCAA CCATCAGCGA GCAACCCCCA GTACGTTCGA GAAGTCGATG
    CAGAATCTCC AGACGAAGAT CCAGGCAAAG AAGGAGCAGG TGGCTGAGGC CAGGGCAGAG
    CTGAGGAGGG CGAGGGCTGA GCACAAAGCC CAAGGGGATG GCAAGTCCAG GAGTGTCCTG
    GAGAAGAAGA GGCGGCTCCT GGAGAAGCTG CAGGAGCAGC TGGCGCAGCT GAGTGTGCAG
    GCCACGGACA AGGAGGAGAA CAAGCAGGTG GCCCTGGGCA CGTCCAAGCT CAACTACCTG
    GACCCCAGGA TCAGCATTGC CTGGTGCAAG CGGTTCAGGG TGCCAGTGGA GAAGATCTAC
    AGCAAAACAC AGCGGGAGAG GTTCGCCTGG GCTCTCGCCA TGGCAGGAGA AGACTTTGAA
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_443195.1
    CDS31..1836
    Translation

    Target ORF information:

    RefSeq Version NM_052963.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase I mitochondrial (TOP1MT), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_052963.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGCGTGG TGCGGCTGCT GCGGCTCCGG GCGGCTCTGA CGCTGCTCGG GGAGGTCCCC 
    CGCCGCCCGG CCTCCCGGGG TGTCCCGGGC TCGCGCAGGA CGCAGAAGGG CAGTGGAGCC
    AGGTGGGAGA AGGAGAAGCA CGAAGACGGG GTGAAGTGGA GACAGCTGGA GCACAAGGGC
    CCGTACTTCG CACCCCCATA CGAGCCCCTT CCCGACGGAG TGCGTTTCTT CTATGAAGGA
    AGGCCTGTGA GATTGAGCGT GGCAGCGGAG GAGGTCGCCA CTTTTTATGG GAGGATGTTA
    GATCATGAAT ACACAACAAA GGAGGTTTTC CGGAAGAACT TCTTCAATGA CTGGCGAAAG
    GAAATGGCGG TGGAAGAGAG GGAAGTCATC AAGAGCCTGG ACAAGTGTGA CTTCACGGAG
    ATCCACAGAT ACTTTGTGGA CAAGGCCGCA GCCCGGAAAG TCCTGAGCAG GGAGGAGAAG
    CAGAAGCTAA AAGAAGAGGC AGAAAAACTT CAGCAAGAGT TCGGCTACTG TATTTTAGAT
    GGTCACCAAG AAAAAATAGG CAACTTCAAG ATTGAGCCGC CTGGCTTGTT CCGTGGCCGT
    GGCGACCATC CCAAGATGGG GATGCTGAAG AGAAGGATCA CGCCAGAGGA TGTGGTTATC
    AACTGCAGCA GGGACTCGAA GATCCCCGAG CCGCCGGCGG GGCACCAGTG GAAGGAGGTG
    CGCTCCGATA ACACCGTCAC GTGGCTGGCA GCTTGGACCG AGAGCGTTCA GAACTCCATC
    AAGTACATCA TGCTGAACCC TTGCTCGAAG CTGAAGGGGG AGACAGCTTG GCAGAAGTTT
    GAAACAGCTC GACGCCTGCG GGGATTTGTG GACGAGATCC GCTCCCAGTA CCGGGCTGAC
    TGGAAGTCTC GGGAAATGAA GACGAGACAG CGGGCGGTGG CCCTGTATTT CATCGATAAG
    CTGGCACTGA GAGCAGGAAA TGAGAAGGAG GACGGTGAGG CGGCCGACAC CGTGGGCTGC
    TGTTCCCTCC GCGTGGAGCA CGTCCAGCTG CACCCGGAGG CCGATGGCTG CCAACACGTG
    GTGGAATTTG ACTTCCTGGG GAAGGACTGC ATCCGCTACT ACAACAGAGT GCCGGTGGAG
    AAGCCGGTGT ACAAGAACTT ACAGCTCTTT ATGGAGAACA AGGACCCCCG GGACGACCTC
    TTCGACAGGC TGACCACGAC CAGCCTGAAC AAGCACCTCC AGGAGCTGAT GGACGGGCTG
    ACGGCCAAGG TGTTCCGGAC CTACAACGCC TCCATCACTC TGCAGGAGCA GCTGCGGGCC
    CTGACGCGCG CCGAGGACAG CATAGCAGCT AAGATCTTAT CCTACAACCG AGCCAACCGA
    GTCGTGGCCA TTCTCTGCAA CCATCAGCGA GCAACCCCCA GTACGTTCGA GAAGTCGATG
    CAGAATCTCC AGACGAAGAT CCAGGCAAAG AAGGAGCAGG TGGCTGAGGC CAGGGCAGAG
    CTGAGGAGGG CGAGGGCTGA GCACAAAGCC CAAGGGGATG GCAAGTCCAG GAGTGTCCTG
    GAGAAGAAGA GGCGGCTCCT GGAGAAGCTG CAGGAGCAGC TGGCGCAGCT GAGTGTGCAG
    GCCACGGACA AGGAGGAGAA CAAGCAGGTG GCCCTGGGCA CGTCCAAGCT CAACTACCTG
    GACCCCAGGA TCAGCATTGC CTGGTGCAAG CGGTTCAGGG TGCCAGTGGA GAAGATCTAC
    AGCAAAACAC AGCGGGAGAG GTTCGCCTGG GCTCTCGCCA TGGCAGGAGA AGACTTTGAA
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10035
    Clone ID Related Accession (Same CDS sequence) NM_052963.2 , NM_052963.3
    Accession Version NM_052963.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product DNA topoisomerase I, mitochondrial isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF349017.1 and AW248607.1. On May 17, 2019 this sequence version replaced NM_052963.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF349017.1, SRR7410570.431963.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000329245.9/ ENSP00000328835.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGCGTGG TGCGGCTGCT GCGGCTCCGG GCGGCTCTGA CGCTGCTCGG GGAGGTCCCC 
    CGCCGCCCGG CCTCCCGGGG TGTCCCGGGC TCGCGCAGGA CGCAGAAGGG CAGTGGAGCC
    AGGTGGGAGA AGGAGAAGCA CGAAGACGGG GTGAAGTGGA GACAGCTGGA GCACAAGGGC
    CCGTACTTCG CACCCCCATA CGAGCCCCTT CCCGACGGAG TGCGTTTCTT CTATGAAGGA
    AGGCCTGTGA GATTGAGCGT GGCAGCGGAG GAGGTCGCCA CTTTTTATGG GAGGATGTTA
    GATCATGAAT ACACAACAAA GGAGGTTTTC CGGAAGAACT TCTTCAATGA CTGGCGAAAG
    GAAATGGCGG TGGAAGAGAG GGAAGTCATC AAGAGCCTGG ACAAGTGTGA CTTCACGGAG
    ATCCACAGAT ACTTTGTGGA CAAGGCCGCA GCCCGGAAAG TCCTGAGCAG GGAGGAGAAG
    CAGAAGCTAA AAGAAGAGGC AGAAAAACTT CAGCAAGAGT TCGGCTACTG TATTTTAGAT
    GGTCACCAAG AAAAAATAGG CAACTTCAAG ATTGAGCCGC CTGGCTTGTT CCGTGGCCGT
    GGCGACCATC CCAAGATGGG GATGCTGAAG AGAAGGATCA CGCCAGAGGA TGTGGTTATC
    AACTGCAGCA GGGACTCGAA GATCCCCGAG CCGCCGGCGG GGCACCAGTG GAAGGAGGTG
    CGCTCCGATA ACACCGTCAC GTGGCTGGCA GCTTGGACCG AGAGCGTTCA GAACTCCATC
    AAGTACATCA TGCTGAACCC TTGCTCGAAG CTGAAGGGGG AGACAGCTTG GCAGAAGTTT
    GAAACAGCTC GACGCCTGCG GGGATTTGTG GACGAGATCC GCTCCCAGTA CCGGGCTGAC
    TGGAAGTCTC GGGAAATGAA GACGAGACAG CGGGCGGTGG CCCTGTATTT CATCGATAAG
    CTGGCACTGA GAGCAGGAAA TGAGAAGGAG GACGGTGAGG CGGCCGACAC CGTGGGCTGC
    TGTTCCCTCC GCGTGGAGCA CGTCCAGCTG CACCCGGAGG CCGATGGCTG CCAACACGTG
    GTGGAATTTG ACTTCCTGGG GAAGGACTGC ATCCGCTACT ACAACAGAGT GCCGGTGGAG
    AAGCCGGTGT ACAAGAACTT ACAGCTCTTT ATGGAGAACA AGGACCCCCG GGACGACCTC
    TTCGACAGGC TGACCACGAC CAGCCTGAAC AAGCACCTCC AGGAGCTGAT GGACGGGCTG
    ACGGCCAAGG TGTTCCGGAC CTACAACGCC TCCATCACTC TGCAGGAGCA GCTGCGGGCC
    CTGACGCGCG CCGAGGACAG CATAGCAGCT AAGATCTTAT CCTACAACCG AGCCAACCGA
    GTCGTGGCCA TTCTCTGCAA CCATCAGCGA GCAACCCCCA GTACGTTCGA GAAGTCGATG
    CAGAATCTCC AGACGAAGAT CCAGGCAAAG AAGGAGCAGG TGGCTGAGGC CAGGGCAGAG
    CTGAGGAGGG CGAGGGCTGA GCACAAAGCC CAAGGGGATG GCAAGTCCAG GAGTGTCCTG
    GAGAAGAAGA GGCGGCTCCT GGAGAAGCTG CAGGAGCAGC TGGCGCAGCT GAGTGTGCAG
    GCCACGGACA AGGAGGAGAA CAAGCAGGTG GCCCTGGGCA CGTCCAAGCT CAACTACCTG
    GACCCCAGGA TCAGCATTGC CTGGTGCAAG CGGTTCAGGG TGCCAGTGGA GAAGATCTAC
    AGCAAAACAC AGCGGGAGAG GTTCGCCTGG GCTCTCGCCA TGGCAGGAGA AGACTTTGAA
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_443195.1
    CDS20..1825
    Translation

    Target ORF information:

    RefSeq Version NM_052963.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase I mitochondrial (TOP1MT), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_052963.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCGCGTGG TGCGGCTGCT GCGGCTCCGG GCGGCTCTGA CGCTGCTCGG GGAGGTCCCC 
    CGCCGCCCGG CCTCCCGGGG TGTCCCGGGC TCGCGCAGGA CGCAGAAGGG CAGTGGAGCC
    AGGTGGGAGA AGGAGAAGCA CGAAGACGGG GTGAAGTGGA GACAGCTGGA GCACAAGGGC
    CCGTACTTCG CACCCCCATA CGAGCCCCTT CCCGACGGAG TGCGTTTCTT CTATGAAGGA
    AGGCCTGTGA GATTGAGCGT GGCAGCGGAG GAGGTCGCCA CTTTTTATGG GAGGATGTTA
    GATCATGAAT ACACAACAAA GGAGGTTTTC CGGAAGAACT TCTTCAATGA CTGGCGAAAG
    GAAATGGCGG TGGAAGAGAG GGAAGTCATC AAGAGCCTGG ACAAGTGTGA CTTCACGGAG
    ATCCACAGAT ACTTTGTGGA CAAGGCCGCA GCCCGGAAAG TCCTGAGCAG GGAGGAGAAG
    CAGAAGCTAA AAGAAGAGGC AGAAAAACTT CAGCAAGAGT TCGGCTACTG TATTTTAGAT
    GGTCACCAAG AAAAAATAGG CAACTTCAAG ATTGAGCCGC CTGGCTTGTT CCGTGGCCGT
    GGCGACCATC CCAAGATGGG GATGCTGAAG AGAAGGATCA CGCCAGAGGA TGTGGTTATC
    AACTGCAGCA GGGACTCGAA GATCCCCGAG CCGCCGGCGG GGCACCAGTG GAAGGAGGTG
    CGCTCCGATA ACACCGTCAC GTGGCTGGCA GCTTGGACCG AGAGCGTTCA GAACTCCATC
    AAGTACATCA TGCTGAACCC TTGCTCGAAG CTGAAGGGGG AGACAGCTTG GCAGAAGTTT
    GAAACAGCTC GACGCCTGCG GGGATTTGTG GACGAGATCC GCTCCCAGTA CCGGGCTGAC
    TGGAAGTCTC GGGAAATGAA GACGAGACAG CGGGCGGTGG CCCTGTATTT CATCGATAAG
    CTGGCACTGA GAGCAGGAAA TGAGAAGGAG GACGGTGAGG CGGCCGACAC CGTGGGCTGC
    TGTTCCCTCC GCGTGGAGCA CGTCCAGCTG CACCCGGAGG CCGATGGCTG CCAACACGTG
    GTGGAATTTG ACTTCCTGGG GAAGGACTGC ATCCGCTACT ACAACAGAGT GCCGGTGGAG
    AAGCCGGTGT ACAAGAACTT ACAGCTCTTT ATGGAGAACA AGGACCCCCG GGACGACCTC
    TTCGACAGGC TGACCACGAC CAGCCTGAAC AAGCACCTCC AGGAGCTGAT GGACGGGCTG
    ACGGCCAAGG TGTTCCGGAC CTACAACGCC TCCATCACTC TGCAGGAGCA GCTGCGGGCC
    CTGACGCGCG CCGAGGACAG CATAGCAGCT AAGATCTTAT CCTACAACCG AGCCAACCGA
    GTCGTGGCCA TTCTCTGCAA CCATCAGCGA GCAACCCCCA GTACGTTCGA GAAGTCGATG
    CAGAATCTCC AGACGAAGAT CCAGGCAAAG AAGGAGCAGG TGGCTGAGGC CAGGGCAGAG
    CTGAGGAGGG CGAGGGCTGA GCACAAAGCC CAAGGGGATG GCAAGTCCAG GAGTGTCCTG
    GAGAAGAAGA GGCGGCTCCT GGAGAAGCTG CAGGAGCAGC TGGCGCAGCT GAGTGTGCAG
    GCCACGGACA AGGAGGAGAA CAAGCAGGTG GCCCTGGGCA CGTCCAAGCT CAACTACCTG
    GACCCCAGGA TCAGCATTGC CTGGTGCAAG CGGTTCAGGG TGCCAGTGGA GAAGATCTAC
    AGCAAAACAC AGCGGGAGAG GTTCGCCTGG GCTCTCGCCA TGGCAGGAGA AGACTTTGAA
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09875
    Clone ID Related Accession (Same CDS sequence) NM_001258447.1 , NM_001258446.1
    Accession Version NM_001258447.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-14
    Organism Homo sapiens(human)
    Product DNA topoisomerase I, mitochondrial isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302372.1, AF349017.1 and AW248607.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302372.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTTAGATC ATGAATACAC AACAAAGGAG GTTTTCCGGA AGAACTTCTT CAATGACTGG 
    CGAAAGGAAA TGGCGGTGGA AGAGAGGGAA GTCATCAAGA GCCTGGACAA GTGTGACTTC
    ACGGAGATCC ACAGATACTT TGTGGACAAG GCCGCAGCCC GGAAAGTCCT GAGCAGGGAG
    GAGAAGCAGA AGCTAAAAGA AGAGGCAGAA AAACTTCAGC AAGAGTTCGG CTACTGTATT
    TTAGATGGTC ACCAAGAAAA AATAGGCAAC TTCAAGATTG AGCCGCCTGG CTTGTTCCGT
    GGCCGTGGCG ACCATCCCAA GATGGGGATG CTGAAGAGAA GGATCACGCC AGAGGATGTG
    GTTATCAACT GCAGCAGGGA CTCGAAGATC CCCGAGCCGC CGGCGGGGCA CCAGTGGAAG
    GAGGTGCGCT CCGATAACAC CGTCACGTGG CTGGCAGCTT GGACCGAGAG CGTTCAGAAC
    TCCATCAAGT ACATCATGCT GAACCCTTGC TCGAAGCTGA AGGGGGAGAC AGCTTGGCAG
    AAGTTTGAAA CAGCTCGACG CCTGCGGGGA TTTGTGGACG AGATCCGCTC CCAGTACCGG
    GCTGACTGGA AGTCTCGGGA AATGAAGACG AGACAGCGGG CGGTGGCCCT GTATTTCATC
    GATAAGCTGG CACTGAGAGC AGGAAATGAG AAGGAGGACG GTGAGGCGGC CGACACCGTG
    GGCTGCTGTT CCCTCCGCGT GGAGCACGTC CAGCTGCACC CGGAGGCCGA TGGCTGCCAA
    CACGTGGTGG AATTTGACTT CCTGGGGAAG GACTGCATCC GCTACTACAA CAGAGTGCCG
    GTGGAGAAGC CGGTGTACAA GAACTTACAG CTCTTTATGG AGAACAAGGA CCCCCGGGAC
    GACCTCTTCG ACAGGCTGAC CACGACCAGC CTGAACAAGC ACCTCCAGGA GCTGATGGAC
    GGGCTGACGG CCAAGGTGTT CCGGACCTAC AACGCCTCCA TCACTCTGCA GGAGCAGCTG
    CGGGCCCTGA CGCGCGCCGA GGACAGCATA GCAGCTAAGA TCTTATCCTA CAACCGAGCC
    AACCGAGTCG TGGCCATTCT CTGCAACCAT CAGCGAGCAA CCCCCAGTAC GTTCGAGAAG
    TCGATGCAGA ATCTCCAGAC GAAGATCCAG GCAAAGAAGG AGCAGGTGGC TGAGGCCAGG
    GCAGAGCTGA GGAGGGCGAG GGCTGAGCAC AAAGCCCAAG GGGATGGCAA GTCCAGGAGT
    GTCCTGGAGA AGAAGAGGCG GCTCCTGGAG AAGCTGCAGG AGCAGCTGGC GCAGCTGAGT
    GTGCAGGCCA CGGACAAGGA GGAGAACAAG CAGGTGGCCC TGGGCACGTC CAAGCTCAAC
    TACCTGGACC CCAGGATCAG CATTGCCTGG TGCAAGCGGT TCAGGGTGCC AGTGGAGAAG
    ATCTACAGCA AAACACAGCG GGAGAGGTTC GCCTGGGCTC TCGCCATGGC AGGAGAAGAC
    TTTGAATTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245376.1
    CDS276..1787
    Translation

    Target ORF information:

    RefSeq Version NM_001258447.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase I mitochondrial (TOP1MT), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTTAGATC ATGAATACAC AACAAAGGAG GTTTTCCGGA AGAACTTCTT CAATGACTGG 
    CGAAAGGAAA TGGCGGTGGA AGAGAGGGAA GTCATCAAGA GCCTGGACAA GTGTGACTTC
    ACGGAGATCC ACAGATACTT TGTGGACAAG GCCGCAGCCC GGAAAGTCCT GAGCAGGGAG
    GAGAAGCAGA AGCTAAAAGA AGAGGCAGAA AAACTTCAGC AAGAGTTCGG CTACTGTATT
    TTAGATGGTC ACCAAGAAAA AATAGGCAAC TTCAAGATTG AGCCGCCTGG CTTGTTCCGT
    GGCCGTGGCG ACCATCCCAA GATGGGGATG CTGAAGAGAA GGATCACGCC AGAGGATGTG
    GTTATCAACT GCAGCAGGGA CTCGAAGATC CCCGAGCCGC CGGCGGGGCA CCAGTGGAAG
    GAGGTGCGCT CCGATAACAC CGTCACGTGG CTGGCAGCTT GGACCGAGAG CGTTCAGAAC
    TCCATCAAGT ACATCATGCT GAACCCTTGC TCGAAGCTGA AGGGGGAGAC AGCTTGGCAG
    AAGTTTGAAA CAGCTCGACG CCTGCGGGGA TTTGTGGACG AGATCCGCTC CCAGTACCGG
    GCTGACTGGA AGTCTCGGGA AATGAAGACG AGACAGCGGG CGGTGGCCCT GTATTTCATC
    GATAAGCTGG CACTGAGAGC AGGAAATGAG AAGGAGGACG GTGAGGCGGC CGACACCGTG
    GGCTGCTGTT CCCTCCGCGT GGAGCACGTC CAGCTGCACC CGGAGGCCGA TGGCTGCCAA
    CACGTGGTGG AATTTGACTT CCTGGGGAAG GACTGCATCC GCTACTACAA CAGAGTGCCG
    GTGGAGAAGC CGGTGTACAA GAACTTACAG CTCTTTATGG AGAACAAGGA CCCCCGGGAC
    GACCTCTTCG ACAGGCTGAC CACGACCAGC CTGAACAAGC ACCTCCAGGA GCTGATGGAC
    GGGCTGACGG CCAAGGTGTT CCGGACCTAC AACGCCTCCA TCACTCTGCA GGAGCAGCTG
    CGGGCCCTGA CGCGCGCCGA GGACAGCATA GCAGCTAAGA TCTTATCCTA CAACCGAGCC
    AACCGAGTCG TGGCCATTCT CTGCAACCAT CAGCGAGCAA CCCCCAGTAC GTTCGAGAAG
    TCGATGCAGA ATCTCCAGAC GAAGATCCAG GCAAAGAAGG AGCAGGTGGC TGAGGCCAGG
    GCAGAGCTGA GGAGGGCGAG GGCTGAGCAC AAAGCCCAAG GGGATGGCAA GTCCAGGAGT
    GTCCTGGAGA AGAAGAGGCG GCTCCTGGAG AAGCTGCAGG AGCAGCTGGC GCAGCTGAGT
    GTGCAGGCCA CGGACAAGGA GGAGAACAAG CAGGTGGCCC TGGGCACGTC CAAGCTCAAC
    TACCTGGACC CCAGGATCAG CATTGCCTGG TGCAAGCGGT TCAGGGTGCC AGTGGAGAAG
    ATCTACAGCA AAACACAGCG GGAGAGGTTC GCCTGGGCTC TCGCCATGGC AGGAGAAGAC
    TTTGAATTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09875
    Clone ID Related Accession (Same CDS sequence) NM_001258447.1 , NM_001258446.1
    Accession Version NM_001258446.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-14
    Organism Homo sapiens(human)
    Product DNA topoisomerase I, mitochondrial isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC399840.1, AK316380.1, AF349017.1 and AW248607.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316380.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTTAGATC ATGAATACAC AACAAAGGAG GTTTTCCGGA AGAACTTCTT CAATGACTGG 
    CGAAAGGAAA TGGCGGTGGA AGAGAGGGAA GTCATCAAGA GCCTGGACAA GTGTGACTTC
    ACGGAGATCC ACAGATACTT TGTGGACAAG GCCGCAGCCC GGAAAGTCCT GAGCAGGGAG
    GAGAAGCAGA AGCTAAAAGA AGAGGCAGAA AAACTTCAGC AAGAGTTCGG CTACTGTATT
    TTAGATGGTC ACCAAGAAAA AATAGGCAAC TTCAAGATTG AGCCGCCTGG CTTGTTCCGT
    GGCCGTGGCG ACCATCCCAA GATGGGGATG CTGAAGAGAA GGATCACGCC AGAGGATGTG
    GTTATCAACT GCAGCAGGGA CTCGAAGATC CCCGAGCCGC CGGCGGGGCA CCAGTGGAAG
    GAGGTGCGCT CCGATAACAC CGTCACGTGG CTGGCAGCTT GGACCGAGAG CGTTCAGAAC
    TCCATCAAGT ACATCATGCT GAACCCTTGC TCGAAGCTGA AGGGGGAGAC AGCTTGGCAG
    AAGTTTGAAA CAGCTCGACG CCTGCGGGGA TTTGTGGACG AGATCCGCTC CCAGTACCGG
    GCTGACTGGA AGTCTCGGGA AATGAAGACG AGACAGCGGG CGGTGGCCCT GTATTTCATC
    GATAAGCTGG CACTGAGAGC AGGAAATGAG AAGGAGGACG GTGAGGCGGC CGACACCGTG
    GGCTGCTGTT CCCTCCGCGT GGAGCACGTC CAGCTGCACC CGGAGGCCGA TGGCTGCCAA
    CACGTGGTGG AATTTGACTT CCTGGGGAAG GACTGCATCC GCTACTACAA CAGAGTGCCG
    GTGGAGAAGC CGGTGTACAA GAACTTACAG CTCTTTATGG AGAACAAGGA CCCCCGGGAC
    GACCTCTTCG ACAGGCTGAC CACGACCAGC CTGAACAAGC ACCTCCAGGA GCTGATGGAC
    GGGCTGACGG CCAAGGTGTT CCGGACCTAC AACGCCTCCA TCACTCTGCA GGAGCAGCTG
    CGGGCCCTGA CGCGCGCCGA GGACAGCATA GCAGCTAAGA TCTTATCCTA CAACCGAGCC
    AACCGAGTCG TGGCCATTCT CTGCAACCAT CAGCGAGCAA CCCCCAGTAC GTTCGAGAAG
    TCGATGCAGA ATCTCCAGAC GAAGATCCAG GCAAAGAAGG AGCAGGTGGC TGAGGCCAGG
    GCAGAGCTGA GGAGGGCGAG GGCTGAGCAC AAAGCCCAAG GGGATGGCAA GTCCAGGAGT
    GTCCTGGAGA AGAAGAGGCG GCTCCTGGAG AAGCTGCAGG AGCAGCTGGC GCAGCTGAGT
    GTGCAGGCCA CGGACAAGGA GGAGAACAAG CAGGTGGCCC TGGGCACGTC CAAGCTCAAC
    TACCTGGACC CCAGGATCAG CATTGCCTGG TGCAAGCGGT TCAGGGTGCC AGTGGAGAAG
    ATCTACAGCA AAACACAGCG GGAGAGGTTC GCCTGGGCTC TCGCCATGGC AGGAGAAGAC
    TTTGAATTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245375.1
    CDS417..1928
    Translation

    Target ORF information:

    RefSeq Version NM_001258446.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase I mitochondrial (TOP1MT), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTTAGATC ATGAATACAC AACAAAGGAG GTTTTCCGGA AGAACTTCTT CAATGACTGG 
    CGAAAGGAAA TGGCGGTGGA AGAGAGGGAA GTCATCAAGA GCCTGGACAA GTGTGACTTC
    ACGGAGATCC ACAGATACTT TGTGGACAAG GCCGCAGCCC GGAAAGTCCT GAGCAGGGAG
    GAGAAGCAGA AGCTAAAAGA AGAGGCAGAA AAACTTCAGC AAGAGTTCGG CTACTGTATT
    TTAGATGGTC ACCAAGAAAA AATAGGCAAC TTCAAGATTG AGCCGCCTGG CTTGTTCCGT
    GGCCGTGGCG ACCATCCCAA GATGGGGATG CTGAAGAGAA GGATCACGCC AGAGGATGTG
    GTTATCAACT GCAGCAGGGA CTCGAAGATC CCCGAGCCGC CGGCGGGGCA CCAGTGGAAG
    GAGGTGCGCT CCGATAACAC CGTCACGTGG CTGGCAGCTT GGACCGAGAG CGTTCAGAAC
    TCCATCAAGT ACATCATGCT GAACCCTTGC TCGAAGCTGA AGGGGGAGAC AGCTTGGCAG
    AAGTTTGAAA CAGCTCGACG CCTGCGGGGA TTTGTGGACG AGATCCGCTC CCAGTACCGG
    GCTGACTGGA AGTCTCGGGA AATGAAGACG AGACAGCGGG CGGTGGCCCT GTATTTCATC
    GATAAGCTGG CACTGAGAGC AGGAAATGAG AAGGAGGACG GTGAGGCGGC CGACACCGTG
    GGCTGCTGTT CCCTCCGCGT GGAGCACGTC CAGCTGCACC CGGAGGCCGA TGGCTGCCAA
    CACGTGGTGG AATTTGACTT CCTGGGGAAG GACTGCATCC GCTACTACAA CAGAGTGCCG
    GTGGAGAAGC CGGTGTACAA GAACTTACAG CTCTTTATGG AGAACAAGGA CCCCCGGGAC
    GACCTCTTCG ACAGGCTGAC CACGACCAGC CTGAACAAGC ACCTCCAGGA GCTGATGGAC
    GGGCTGACGG CCAAGGTGTT CCGGACCTAC AACGCCTCCA TCACTCTGCA GGAGCAGCTG
    CGGGCCCTGA CGCGCGCCGA GGACAGCATA GCAGCTAAGA TCTTATCCTA CAACCGAGCC
    AACCGAGTCG TGGCCATTCT CTGCAACCAT CAGCGAGCAA CCCCCAGTAC GTTCGAGAAG
    TCGATGCAGA ATCTCCAGAC GAAGATCCAG GCAAAGAAGG AGCAGGTGGC TGAGGCCAGG
    GCAGAGCTGA GGAGGGCGAG GGCTGAGCAC AAAGCCCAAG GGGATGGCAA GTCCAGGAGT
    GTCCTGGAGA AGAAGAGGCG GCTCCTGGAG AAGCTGCAGG AGCAGCTGGC GCAGCTGAGT
    GTGCAGGCCA CGGACAAGGA GGAGAACAAG CAGGTGGCCC TGGGCACGTC CAAGCTCAAC
    TACCTGGACC CCAGGATCAG CATTGCCTGG TGCAAGCGGT TCAGGGTGCC AGTGGAGAAG
    ATCTACAGCA AAACACAGCG GGAGAGGTTC GCCTGGGCTC TCGCCATGGC AGGAGAAGAC
    TTTGAATTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu42348
    Clone ID Related Accession (Same CDS sequence) XM_005250781.4 , XM_005250781.3
    Accession Version XM_005250781.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase I, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005250781.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCGCGTGG TGCGGCTGCT GCGGCTCCGG GCGGCTCTGA CGCTGCTCGG GGAGGTCCCC 
    CGCCGCCCGG CCTCCCGGGG TGTCCCGGGC TCGCGCAGGA CGCAGAAGGG CAGTGGAGCC
    AGGTGGGAGA AGGAGAAGCA CGAAGACGGG GTGAAGTGGA GACAGCTGGA GCACAAGGGC
    CCGTACTTCG CACCCCCATA CGAGCCCCTT CCCGACGGAG TGCGTTTCTT CTATGAAGGA
    AGGCCTGTGA GATTGAGCGT GGCAGCGGAG GAGGTCGCCA CTTTTTATGG GAGGATGTTA
    GATCATGAAT ACACAACAAA GGAGGTTTTC CGGAAGAACT TCTTCAATGA CTGGCGAAAG
    GAAATGGCGG TGGAAGAGAG GGAAGTCATC AAGAGCCTGG ACAAGTGTGA CTTCACGGAG
    ATCCACAGAT ACTTTGTGGA CAAGGCCGCA GCCCGGAAAG TCCTGAGCAG GGAGGAGAAG
    CAGAAGCTAA AAGAAGAGGC AGAAAAACTT CAGCAAGAGT TCGGCTACTG TATTTTAGAT
    GGTCACCAAG AAAAAATAGG CAACTTCAAG ATTGAGCCGC CTGGCTTGTT CCGTGGCCGT
    GGCGACCATC CCAAGATGGG GATGCTGAAG AGAAGGATCA CGCCAGAGGA TGTGGTTATC
    AACTGCAGCA GGGACTCGAA GATCCCCGAG CCGCCGGCGG GGCACCAGTG GAAGGAGGTG
    CGCTCCGATA ACACCGTCAC GTGGCTGGCA GCTTGGACCG AGAGCGTTCA GAACTCCATC
    AAGTACATCA TGCTGAACCC TTGCTCGAAG CTGAAGGGGG AGACAGCTTG GCAGAAGTTT
    GAAACAGCTC GACGCCTGCG GGGATTTGTG GACGAGATCC GCTCCCAGTA CCGGGCTGAC
    TGGAAGTCTC GGGAAATGAA GACGAGACAG CGGGCGGTGG CCCTGTATTT CATCGATAAG
    CTGGCACTGA GAGCAGGAAA TGAGAAGGAG GACGGTGAGG CGGCCGACAC CGTGGGCTGC
    TGTTCCCTCC GCGTGGAGCA CGTCCAGCTG CACCCGGAGG CCGATGGCTG CCAACACGTG
    GTGGAATTTG ACTTCCTGGG GAAGGACTGC ATCCGCTACT ACAACAGAGT GCCGGTGGAG
    AAGCCGGTGT ACAAGAACTT ACAGCTCTTT ATGGAGAACA AGGACCCCCG GGACGACCTC
    TTCGACAGGC TGACCACGAC CAGCCTGAAC AAGCACCTCC AGGAGCTGAT GGACGGGCTG
    ACGGCCAAGG TGTTCCGGAC CTACAACGCC TCCATCACTC TGCAGGAGCA GCTGCGGGCC
    CTGACGCGCG CCGAGGACAG CATAGCAGCT AAGATCTTAT CCTACAACCG AGCCAACCGA
    GTCGTGGCCA TTCTCTGCAA CCATCAGCGA GCAACCCCCA GTACGTTCGA GAAGTCGATG
    CAGAATCTCC AGACGAAGAT CCAGGCAAAG AAGGAGCAGG TGGCTGAGGC CAGGGCAGAG
    CTGAGGAGGG CGAGGGCTGA GCACAAAGCC CAAGGGGATG GCAAGTCCAG GAGTGTCCTG
    GAGAAGAAGA GGCGGCTCCT GGAGAAGCTG CAGGAGCAGC TGGCGCAGCT GAGTGTGCAG
    GCCACGGACA AGGAGGAGAA CAAGCAGGTG GCCCTGGGCA CGTCCAAGCT CAACTACCTG
    GACCCCAGGA TCAGCATTGC CTGGTGCGTG TGCCTGGGGT CTCAGTTCCC ACCTGTGGCC
    TATGGGGGGC CTGGGTTTTC CAGGAGGCCT CTGTTCAGTC CCAGCATTGC CCTTGTCCCG
    TGTCCCCTGG GGTCAGCACA GGCCCTCCCT GTGACAGGGA CCCATGATGC TGAGTCCTGT
    GTCATCAGCT CTCCCTGCCC TGAACGTTCG GATGGACAGG GTGAAGTGGG GGTGCTGACA
    GTGGCCTGGG ACTCGGAGGC ATCAGGAGGG CCTGTGGAGC TCAGCCTGCC CACAGGGAGT
    GGGAGGTGGC TGAGCGCCCT GCTCGGTGGA CACCTAGGCC ACAGCAGGTT GTCTTTGACA
    CCTCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250838.1
    CDS20..2068
    Translation

    Target ORF information:

    RefSeq Version XM_005250781.4
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase I mitochondrial (TOP1MT), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005250781.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCGCGTGG TGCGGCTGCT GCGGCTCCGG GCGGCTCTGA CGCTGCTCGG GGAGGTCCCC 
    CGCCGCCCGG CCTCCCGGGG TGTCCCGGGC TCGCGCAGGA CGCAGAAGGG CAGTGGAGCC
    AGGTGGGAGA AGGAGAAGCA CGAAGACGGG GTGAAGTGGA GACAGCTGGA GCACAAGGGC
    CCGTACTTCG CACCCCCATA CGAGCCCCTT CCCGACGGAG TGCGTTTCTT CTATGAAGGA
    AGGCCTGTGA GATTGAGCGT GGCAGCGGAG GAGGTCGCCA CTTTTTATGG GAGGATGTTA
    GATCATGAAT ACACAACAAA GGAGGTTTTC CGGAAGAACT TCTTCAATGA CTGGCGAAAG
    GAAATGGCGG TGGAAGAGAG GGAAGTCATC AAGAGCCTGG ACAAGTGTGA CTTCACGGAG
    ATCCACAGAT ACTTTGTGGA CAAGGCCGCA GCCCGGAAAG TCCTGAGCAG GGAGGAGAAG
    CAGAAGCTAA AAGAAGAGGC AGAAAAACTT CAGCAAGAGT TCGGCTACTG TATTTTAGAT
    GGTCACCAAG AAAAAATAGG CAACTTCAAG ATTGAGCCGC CTGGCTTGTT CCGTGGCCGT
    GGCGACCATC CCAAGATGGG GATGCTGAAG AGAAGGATCA CGCCAGAGGA TGTGGTTATC
    AACTGCAGCA GGGACTCGAA GATCCCCGAG CCGCCGGCGG GGCACCAGTG GAAGGAGGTG
    CGCTCCGATA ACACCGTCAC GTGGCTGGCA GCTTGGACCG AGAGCGTTCA GAACTCCATC
    AAGTACATCA TGCTGAACCC TTGCTCGAAG CTGAAGGGGG AGACAGCTTG GCAGAAGTTT
    GAAACAGCTC GACGCCTGCG GGGATTTGTG GACGAGATCC GCTCCCAGTA CCGGGCTGAC
    TGGAAGTCTC GGGAAATGAA GACGAGACAG CGGGCGGTGG CCCTGTATTT CATCGATAAG
    CTGGCACTGA GAGCAGGAAA TGAGAAGGAG GACGGTGAGG CGGCCGACAC CGTGGGCTGC
    TGTTCCCTCC GCGTGGAGCA CGTCCAGCTG CACCCGGAGG CCGATGGCTG CCAACACGTG
    GTGGAATTTG ACTTCCTGGG GAAGGACTGC ATCCGCTACT ACAACAGAGT GCCGGTGGAG
    AAGCCGGTGT ACAAGAACTT ACAGCTCTTT ATGGAGAACA AGGACCCCCG GGACGACCTC
    TTCGACAGGC TGACCACGAC CAGCCTGAAC AAGCACCTCC AGGAGCTGAT GGACGGGCTG
    ACGGCCAAGG TGTTCCGGAC CTACAACGCC TCCATCACTC TGCAGGAGCA GCTGCGGGCC
    CTGACGCGCG CCGAGGACAG CATAGCAGCT AAGATCTTAT CCTACAACCG AGCCAACCGA
    GTCGTGGCCA TTCTCTGCAA CCATCAGCGA GCAACCCCCA GTACGTTCGA GAAGTCGATG
    CAGAATCTCC AGACGAAGAT CCAGGCAAAG AAGGAGCAGG TGGCTGAGGC CAGGGCAGAG
    CTGAGGAGGG CGAGGGCTGA GCACAAAGCC CAAGGGGATG GCAAGTCCAG GAGTGTCCTG
    GAGAAGAAGA GGCGGCTCCT GGAGAAGCTG CAGGAGCAGC TGGCGCAGCT GAGTGTGCAG
    GCCACGGACA AGGAGGAGAA CAAGCAGGTG GCCCTGGGCA CGTCCAAGCT CAACTACCTG
    GACCCCAGGA TCAGCATTGC CTGGTGCGTG TGCCTGGGGT CTCAGTTCCC ACCTGTGGCC
    TATGGGGGGC CTGGGTTTTC CAGGAGGCCT CTGTTCAGTC CCAGCATTGC CCTTGTCCCG
    TGTCCCCTGG GGTCAGCACA GGCCCTCCCT GTGACAGGGA CCCATGATGC TGAGTCCTGT
    GTCATCAGCT CTCCCTGCCC TGAACGTTCG GATGGACAGG GTGAAGTGGG GGTGCTGACA
    GTGGCCTGGG ACTCGGAGGC ATCAGGAGGG CCTGTGGAGC TCAGCCTGCC CACAGGGAGT
    GGGAGGTGGC TGAGCGCCCT GCTCGGTGGA CACCTAGGCC ACAGCAGGTT GTCTTTGACA
    CCTCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu42348
    Clone ID Related Accession (Same CDS sequence) XM_005250781.4 , XM_005250781.3
    Accession Version XM_005250781.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase I, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005250781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCGCGTGG TGCGGCTGCT GCGGCTCCGG GCGGCTCTGA CGCTGCTCGG GGAGGTCCCC 
    CGCCGCCCGG CCTCCCGGGG TGTCCCGGGC TCGCGCAGGA CGCAGAAGGG CAGTGGAGCC
    AGGTGGGAGA AGGAGAAGCA CGAAGACGGG GTGAAGTGGA GACAGCTGGA GCACAAGGGC
    CCGTACTTCG CACCCCCATA CGAGCCCCTT CCCGACGGAG TGCGTTTCTT CTATGAAGGA
    AGGCCTGTGA GATTGAGCGT GGCAGCGGAG GAGGTCGCCA CTTTTTATGG GAGGATGTTA
    GATCATGAAT ACACAACAAA GGAGGTTTTC CGGAAGAACT TCTTCAATGA CTGGCGAAAG
    GAAATGGCGG TGGAAGAGAG GGAAGTCATC AAGAGCCTGG ACAAGTGTGA CTTCACGGAG
    ATCCACAGAT ACTTTGTGGA CAAGGCCGCA GCCCGGAAAG TCCTGAGCAG GGAGGAGAAG
    CAGAAGCTAA AAGAAGAGGC AGAAAAACTT CAGCAAGAGT TCGGCTACTG TATTTTAGAT
    GGTCACCAAG AAAAAATAGG CAACTTCAAG ATTGAGCCGC CTGGCTTGTT CCGTGGCCGT
    GGCGACCATC CCAAGATGGG GATGCTGAAG AGAAGGATCA CGCCAGAGGA TGTGGTTATC
    AACTGCAGCA GGGACTCGAA GATCCCCGAG CCGCCGGCGG GGCACCAGTG GAAGGAGGTG
    CGCTCCGATA ACACCGTCAC GTGGCTGGCA GCTTGGACCG AGAGCGTTCA GAACTCCATC
    AAGTACATCA TGCTGAACCC TTGCTCGAAG CTGAAGGGGG AGACAGCTTG GCAGAAGTTT
    GAAACAGCTC GACGCCTGCG GGGATTTGTG GACGAGATCC GCTCCCAGTA CCGGGCTGAC
    TGGAAGTCTC GGGAAATGAA GACGAGACAG CGGGCGGTGG CCCTGTATTT CATCGATAAG
    CTGGCACTGA GAGCAGGAAA TGAGAAGGAG GACGGTGAGG CGGCCGACAC CGTGGGCTGC
    TGTTCCCTCC GCGTGGAGCA CGTCCAGCTG CACCCGGAGG CCGATGGCTG CCAACACGTG
    GTGGAATTTG ACTTCCTGGG GAAGGACTGC ATCCGCTACT ACAACAGAGT GCCGGTGGAG
    AAGCCGGTGT ACAAGAACTT ACAGCTCTTT ATGGAGAACA AGGACCCCCG GGACGACCTC
    TTCGACAGGC TGACCACGAC CAGCCTGAAC AAGCACCTCC AGGAGCTGAT GGACGGGCTG
    ACGGCCAAGG TGTTCCGGAC CTACAACGCC TCCATCACTC TGCAGGAGCA GCTGCGGGCC
    CTGACGCGCG CCGAGGACAG CATAGCAGCT AAGATCTTAT CCTACAACCG AGCCAACCGA
    GTCGTGGCCA TTCTCTGCAA CCATCAGCGA GCAACCCCCA GTACGTTCGA GAAGTCGATG
    CAGAATCTCC AGACGAAGAT CCAGGCAAAG AAGGAGCAGG TGGCTGAGGC CAGGGCAGAG
    CTGAGGAGGG CGAGGGCTGA GCACAAAGCC CAAGGGGATG GCAAGTCCAG GAGTGTCCTG
    GAGAAGAAGA GGCGGCTCCT GGAGAAGCTG CAGGAGCAGC TGGCGCAGCT GAGTGTGCAG
    GCCACGGACA AGGAGGAGAA CAAGCAGGTG GCCCTGGGCA CGTCCAAGCT CAACTACCTG
    GACCCCAGGA TCAGCATTGC CTGGTGCGTG TGCCTGGGGT CTCAGTTCCC ACCTGTGGCC
    TATGGGGGGC CTGGGTTTTC CAGGAGGCCT CTGTTCAGTC CCAGCATTGC CCTTGTCCCG
    TGTCCCCTGG GGTCAGCACA GGCCCTCCCT GTGACAGGGA CCCATGATGC TGAGTCCTGT
    GTCATCAGCT CTCCCTGCCC TGAACGTTCG GATGGACAGG GTGAAGTGGG GGTGCTGACA
    GTGGCCTGGG ACTCGGAGGC ATCAGGAGGG CCTGTGGAGC TCAGCCTGCC CACAGGGAGT
    GGGAGGTGGC TGAGCGCCCT GCTCGGTGGA CACCTAGGCC ACAGCAGGTT GTCTTTGACA
    CCTCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250838.1
    CDS19..2067
    Translation

    Target ORF information:

    RefSeq Version XM_005250781.3
    Organism Homo sapiens(human)
    Definition Homo sapiens topoisomerase (DNA) I, mitochondrial (TOP1MT), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005250781.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGCGCGTGG TGCGGCTGCT GCGGCTCCGG GCGGCTCTGA CGCTGCTCGG GGAGGTCCCC 
    CGCCGCCCGG CCTCCCGGGG TGTCCCGGGC TCGCGCAGGA CGCAGAAGGG CAGTGGAGCC
    AGGTGGGAGA AGGAGAAGCA CGAAGACGGG GTGAAGTGGA GACAGCTGGA GCACAAGGGC
    CCGTACTTCG CACCCCCATA CGAGCCCCTT CCCGACGGAG TGCGTTTCTT CTATGAAGGA
    AGGCCTGTGA GATTGAGCGT GGCAGCGGAG GAGGTCGCCA CTTTTTATGG GAGGATGTTA
    GATCATGAAT ACACAACAAA GGAGGTTTTC CGGAAGAACT TCTTCAATGA CTGGCGAAAG
    GAAATGGCGG TGGAAGAGAG GGAAGTCATC AAGAGCCTGG ACAAGTGTGA CTTCACGGAG
    ATCCACAGAT ACTTTGTGGA CAAGGCCGCA GCCCGGAAAG TCCTGAGCAG GGAGGAGAAG
    CAGAAGCTAA AAGAAGAGGC AGAAAAACTT CAGCAAGAGT TCGGCTACTG TATTTTAGAT
    GGTCACCAAG AAAAAATAGG CAACTTCAAG ATTGAGCCGC CTGGCTTGTT CCGTGGCCGT
    GGCGACCATC CCAAGATGGG GATGCTGAAG AGAAGGATCA CGCCAGAGGA TGTGGTTATC
    AACTGCAGCA GGGACTCGAA GATCCCCGAG CCGCCGGCGG GGCACCAGTG GAAGGAGGTG
    CGCTCCGATA ACACCGTCAC GTGGCTGGCA GCTTGGACCG AGAGCGTTCA GAACTCCATC
    AAGTACATCA TGCTGAACCC TTGCTCGAAG CTGAAGGGGG AGACAGCTTG GCAGAAGTTT
    GAAACAGCTC GACGCCTGCG GGGATTTGTG GACGAGATCC GCTCCCAGTA CCGGGCTGAC
    TGGAAGTCTC GGGAAATGAA GACGAGACAG CGGGCGGTGG CCCTGTATTT CATCGATAAG
    CTGGCACTGA GAGCAGGAAA TGAGAAGGAG GACGGTGAGG CGGCCGACAC CGTGGGCTGC
    TGTTCCCTCC GCGTGGAGCA CGTCCAGCTG CACCCGGAGG CCGATGGCTG CCAACACGTG
    GTGGAATTTG ACTTCCTGGG GAAGGACTGC ATCCGCTACT ACAACAGAGT GCCGGTGGAG
    AAGCCGGTGT ACAAGAACTT ACAGCTCTTT ATGGAGAACA AGGACCCCCG GGACGACCTC
    TTCGACAGGC TGACCACGAC CAGCCTGAAC AAGCACCTCC AGGAGCTGAT GGACGGGCTG
    ACGGCCAAGG TGTTCCGGAC CTACAACGCC TCCATCACTC TGCAGGAGCA GCTGCGGGCC
    CTGACGCGCG CCGAGGACAG CATAGCAGCT AAGATCTTAT CCTACAACCG AGCCAACCGA
    GTCGTGGCCA TTCTCTGCAA CCATCAGCGA GCAACCCCCA GTACGTTCGA GAAGTCGATG
    CAGAATCTCC AGACGAAGAT CCAGGCAAAG AAGGAGCAGG TGGCTGAGGC CAGGGCAGAG
    CTGAGGAGGG CGAGGGCTGA GCACAAAGCC CAAGGGGATG GCAAGTCCAG GAGTGTCCTG
    GAGAAGAAGA GGCGGCTCCT GGAGAAGCTG CAGGAGCAGC TGGCGCAGCT GAGTGTGCAG
    GCCACGGACA AGGAGGAGAA CAAGCAGGTG GCCCTGGGCA CGTCCAAGCT CAACTACCTG
    GACCCCAGGA TCAGCATTGC CTGGTGCGTG TGCCTGGGGT CTCAGTTCCC ACCTGTGGCC
    TATGGGGGGC CTGGGTTTTC CAGGAGGCCT CTGTTCAGTC CCAGCATTGC CCTTGTCCCG
    TGTCCCCTGG GGTCAGCACA GGCCCTCCCT GTGACAGGGA CCCATGATGC TGAGTCCTGT
    GTCATCAGCT CTCCCTGCCC TGAACGTTCG GATGGACAGG GTGAAGTGGG GGTGCTGACA
    GTGGCCTGGG ACTCGGAGGC ATCAGGAGGG CCTGTGGAGC TCAGCCTGCC CACAGGGAGT
    GGGAGGTGGC TGAGCGCCCT GCTCGGTGGA CACCTAGGCC ACAGCAGGTT GTCTTTGACA
    CCTCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.