ORF » Species Summary » Xenopus tropicalis » top1mt cDNA ORF clone

top1mt cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following top1mt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top1mt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa19271 XM_004919867.4
Latest version!		 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OXa19269 XM_031903848.1
Latest version!		 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa19270 XM_031903852.1
Latest version!		 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa19270 XM_031903851.1
Latest version!		 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa19270 XM_031903850.1
Latest version!		 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa60242 XM_031903849.1
Latest version!		 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa19269 XM_004919865.3
Latest version!		 Xenopus tropicalis topoisomerase (DNA) I, mitochondrial (top1mt), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OXa19270 XM_004919866.3
Latest version!		 Xenopus tropicalis topoisomerase (DNA) I, mitochondrial (top1mt), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OXa19271 XM_004919867.3 Xenopus tropicalis topoisomerase (DNA) I, mitochondrial (top1mt), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    top1mt ( XM_004919867.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004919924.1 Xenopus tropicalis topoisomerase (DNA) I, mitochondrial (top1mt), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OXa19271
    Clone ID Related Accession (Same CDS sequence) XM_004919867.3 , XM_004919867.4
    Accession Version XM_004919867.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase I, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004919867.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTGTACC GAGGCTGCAG GATTCTCCTT ACACTTAGTG CCGGGACTAG ATGGGCCCCC 
    CGTGTGTGGC CTCAACTGAG GGGGGTGTGC ATGGCAAGCA CTCGGGCATC TCCTGCCGTG 
    TTTAGGTGGG AGGAGGAGCA CTACGAGGAT GGCATGAAGT GGAAATTCCT GGAACACAGG 
    GGCCCATATT TTGCCCCTCC GTATGAGCCC CTCCCGGATG ATGTCAGCTT CTACTATGAT 
    GGGAAGCCAG TGAAACTGAG CCCAGGGGCC GAGGAAGTGG CCACTTTCTA TGGCAAAATG 
    CTGGACCATG AGTATACTAC CAAGGAGGCG TTCCAGAAGA ACTTCTTTGC AGACTGGAGA 
    GAGCAAATGA CGAATGAGGA GCGGAAGCTG ATCAAGCACC TACACAAGTG TGAATTCTCC 
    GAGATCCACA AGTATTTCTT TGAAAAGAAT GAGGCCCGCA AGGCTTTGCC CAAGGAAGAG 
    AAACAGAAAC TGAAGGAGGA AACCAATAAG ATCCAGGAGG AGTACGGCTA CTGCATCCTT 
    GATGGACACA GGGAAAAAAT TGGAAACTTC AAGATCGAGC CCCCAGGACT GTTCCGAGGC 
    CGTGGGGACC ACCCAAAGAT GGGAATGCTG AAGCTGAGGA TAATGCCAGA GGATGTGATT 
    ATAAACTGCA GCAAAGAATC CAAGGTTCCA GACCCCCCTC CGGGCCACAA GTGGAAAGAG 
    GTTCGGTGCG ACAACACCGT GACATGGCTG GCATCATGGA CGGAAAATAT CCAGAGTTCT 
    ATTAAGTACA TCATGCTGAA CCCAAGCTCC AAACTGAAGG GGGAGAAAGA CTGGCAGAAG 
    TACGAGACTG CTCGCCGACT GAAAGGGGTT GTGAGCGACA TCAGAGCTCA GTACCGAGTA 
    GACTGGAAAT CCCGGGAGAT GAAGAAACGG CAGAGAGCTG TGGCCCTCTA TTTCATTGAT 
    AAGCTGGCAC TCCGAGCGGG AAATGAAAAA GAGGAAGGGG AGACGGCAGA TACAGTTGGC 
    TGCTGTTCCC TTAGGGTGGA GCACATACAG CTGTACCCAG AGCTCGATGG GCAGCAGTAC 
    GTGGTGGAGT TTGACTTCCT GGGAAAGGAT TCCATCCGCT ATTATAACAA GGTGCCAGTG 
    GAGAAGGAGG TGTTTAAGAA CCTGAAGCTT TTCATGGAGA ATAAAAACCC GGAGGATGAT 
    CTTTTCGACA GGCTGAATAC TTCCATACTG AACAAACATC TACAGGATCT GATGGATGGA 
    CTTACGGCCA AAGTGTTCCG AACCTACAAT GCTTCTATCA CCCTGCAGAA CCAGCTGAAG 
    GAGCTGACCA ATGGGGAGGA CAATGTAGCG GCCAAGATTC TGTCTTATAA TCGGGCAAAC 
    AGAGCTGTAG CTCTTCTCTG TAACCACCAG AGGGCGCCAC CAAAAACCTT TGAAAAGTCA 
    ATGCAGAATC TACAGACTAA GATTGATGCA AAGGAACAGC AGCTGAAAGA AGCAAAGAAA 
    GAGTTAAAGA GCGCAAAGGC AGAGCATAAA GCCAGGAAGA GTGAGAAGTC TAAGAAGGAG 
    ATGGAGAAGA AGAAGAAGCA AGTGGCACGG ATAGAGGAGC AGCTGATGAA GCTGAAGTTA 
    CAGGCGACCG ACAAAGAGGA GAATAAGCAG ATTGCCCTGG GCACGTCCAA ACTCAACTAT 
    CTGGATCCCA GGATCACCGT AGCCTGGTGC AAGAAGTACG GCGTGCCCAT CGAGAAGATC 
    TATAACAAGA CGCAGAGAGA GAAATTTGCG TGGGCGATAG ACATGACCAG CGAGAACTTT 
    GAGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004919924.1
    CDS82..1890
    Translation

    Target ORF information:

    RefSeq Version XM_004919867.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) I, mitochondrial (top1mt), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004919867.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTGTACC GAGGCTGCAG GATTCTCCTT ACACTTAGTG CCGGGACTAG ATGGGCCCCC 
    CGTGTGTGGC CTCAACTGAG GGGGGTGTGC ATGGCAAGCA CTCGGGCATC TCCTGCCGTG 
    TTTAGGTGGG AGGAGGAGCA CTACGAGGAT GGCATGAAGT GGAAATTCCT GGAACACAGG 
    GGCCCATATT TTGCCCCTCC GTATGAGCCC CTCCCGGATG ATGTCAGCTT CTACTATGAT 
    GGGAAGCCAG TGAAACTGAG CCCAGGGGCC GAGGAAGTGG CCACTTTCTA TGGCAAAATG 
    CTGGACCATG AGTATACTAC CAAGGAGGCG TTCCAGAAGA ACTTCTTTGC AGACTGGAGA 
    GAGCAAATGA CGAATGAGGA GCGGAAGCTG ATCAAGCACC TACACAAGTG TGAATTCTCC 
    GAGATCCACA AGTATTTCTT TGAAAAGAAT GAGGCCCGCA AGGCTTTGCC CAAGGAAGAG 
    AAACAGAAAC TGAAGGAGGA AACCAATAAG ATCCAGGAGG AGTACGGCTA CTGCATCCTT 
    GATGGACACA GGGAAAAAAT TGGAAACTTC AAGATCGAGC CCCCAGGACT GTTCCGAGGC 
    CGTGGGGACC ACCCAAAGAT GGGAATGCTG AAGCTGAGGA TAATGCCAGA GGATGTGATT 
    ATAAACTGCA GCAAAGAATC CAAGGTTCCA GACCCCCCTC CGGGCCACAA GTGGAAAGAG 
    GTTCGGTGCG ACAACACCGT GACATGGCTG GCATCATGGA CGGAAAATAT CCAGAGTTCT 
    ATTAAGTACA TCATGCTGAA CCCAAGCTCC AAACTGAAGG GGGAGAAAGA CTGGCAGAAG 
    TACGAGACTG CTCGCCGACT GAAAGGGGTT GTGAGCGACA TCAGAGCTCA GTACCGAGTA 
    GACTGGAAAT CCCGGGAGAT GAAGAAACGG CAGAGAGCTG TGGCCCTCTA TTTCATTGAT 
    AAGCTGGCAC TCCGAGCGGG AAATGAAAAA GAGGAAGGGG AGACGGCAGA TACAGTTGGC 
    TGCTGTTCCC TTAGGGTGGA GCACATACAG CTGTACCCAG AGCTCGATGG GCAGCAGTAC 
    GTGGTGGAGT TTGACTTCCT GGGAAAGGAT TCCATCCGCT ATTATAACAA GGTGCCAGTG 
    GAGAAGGAGG TGTTTAAGAA CCTGAAGCTT TTCATGGAGA ATAAAAACCC GGAGGATGAT 
    CTTTTCGACA GGCTGAATAC TTCCATACTG AACAAACATC TACAGGATCT GATGGATGGA 
    CTTACGGCCA AAGTGTTCCG AACCTACAAT GCTTCTATCA CCCTGCAGAA CCAGCTGAAG 
    GAGCTGACCA ATGGGGAGGA CAATGTAGCG GCCAAGATTC TGTCTTATAA TCGGGCAAAC 
    AGAGCTGTAG CTCTTCTCTG TAACCACCAG AGGGCGCCAC CAAAAACCTT TGAAAAGTCA 
    ATGCAGAATC TACAGACTAA GATTGATGCA AAGGAACAGC AGCTGAAAGA AGCAAAGAAA 
    GAGTTAAAGA GCGCAAAGGC AGAGCATAAA GCCAGGAAGA GTGAGAAGTC TAAGAAGGAG 
    ATGGAGAAGA AGAAGAAGCA AGTGGCACGG ATAGAGGAGC AGCTGATGAA GCTGAAGTTA 
    CAGGCGACCG ACAAAGAGGA GAATAAGCAG ATTGCCCTGG GCACGTCCAA ACTCAACTAT 
    CTGGATCCCA GGATCACCGT AGCCTGGTGC AAGAAGTACG GCGTGCCCAT CGAGAAGATC 
    TATAACAAGA CGCAGAGAGA GAAATTTGCG TGGGCGATAG ACATGACCAG CGAGAACTTT 
    GAGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    top1mt ( XM_004919867.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004919924.1 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OXa19271
    Clone ID Related Accession (Same CDS sequence) XM_004919867.3 , XM_004919867.4
    Accession Version XM_004919867.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase I, mitochondrial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004919867.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTGTACC GAGGCTGCAG GATTCTCCTT ACACTTAGTG CCGGGACTAG ATGGGCCCCC 
    CGTGTGTGGC CTCAACTGAG GGGGGTGTGC ATGGCAAGCA CTCGGGCATC TCCTGCCGTG 
    TTTAGGTGGG AGGAGGAGCA CTACGAGGAT GGCATGAAGT GGAAATTCCT GGAACACAGG 
    GGCCCATATT TTGCCCCTCC GTATGAGCCC CTCCCGGATG ATGTCAGCTT CTACTATGAT 
    GGGAAGCCAG TGAAACTGAG CCCAGGGGCC GAGGAAGTGG CCACTTTCTA TGGCAAAATG 
    CTGGACCATG AGTATACTAC CAAGGAGGCG TTCCAGAAGA ACTTCTTTGC AGACTGGAGA 
    GAGCAAATGA CGAATGAGGA GCGGAAGCTG ATCAAGCACC TACACAAGTG TGAATTCTCC 
    GAGATCCACA AGTATTTCTT TGAAAAGAAT GAGGCCCGCA AGGCTTTGCC CAAGGAAGAG 
    AAACAGAAAC TGAAGGAGGA AACCAATAAG ATCCAGGAGG AGTACGGCTA CTGCATCCTT 
    GATGGACACA GGGAAAAAAT TGGAAACTTC AAGATCGAGC CCCCAGGACT GTTCCGAGGC 
    CGTGGGGACC ACCCAAAGAT GGGAATGCTG AAGCTGAGGA TAATGCCAGA GGATGTGATT 
    ATAAACTGCA GCAAAGAATC CAAGGTTCCA GACCCCCCTC CGGGCCACAA GTGGAAAGAG 
    GTTCGGTGCG ACAACACCGT GACATGGCTG GCATCATGGA CGGAAAATAT CCAGAGTTCT 
    ATTAAGTACA TCATGCTGAA CCCAAGCTCC AAACTGAAGG GGGAGAAAGA CTGGCAGAAG 
    TACGAGACTG CTCGCCGACT GAAAGGGGTT GTGAGCGACA TCAGAGCTCA GTACCGAGTA 
    GACTGGAAAT CCCGGGAGAT GAAGAAACGG CAGAGAGCTG TGGCCCTCTA TTTCATTGAT 
    AAGCTGGCAC TCCGAGCGGG AAATGAAAAA GAGGAAGGGG AGACGGCAGA TACAGTTGGC 
    TGCTGTTCCC TTAGGGTGGA GCACATACAG CTGTACCCAG AGCTCGATGG GCAGCAGTAC 
    GTGGTGGAGT TTGACTTCCT GGGAAAGGAT TCCATCCGCT ATTATAACAA GGTGCCAGTG 
    GAGAAGGAGG TGTTTAAGAA CCTGAAGCTT TTCATGGAGA ATAAAAACCC GGAGGATGAT 
    CTTTTCGACA GGCTGAATAC TTCCATACTG AACAAACATC TACAGGATCT GATGGATGGA 
    CTTACGGCCA AAGTGTTCCG AACCTACAAT GCTTCTATCA CCCTGCAGAA CCAGCTGAAG 
    GAGCTGACCA ATGGGGAGGA CAATGTAGCG GCCAAGATTC TGTCTTATAA TCGGGCAAAC 
    AGAGCTGTAG CTCTTCTCTG TAACCACCAG AGGGCGCCAC CAAAAACCTT TGAAAAGTCA 
    ATGCAGAATC TACAGACTAA GATTGATGCA AAGGAACAGC AGCTGAAAGA AGCAAAGAAA 
    GAGTTAAAGA GCGCAAAGGC AGAGCATAAA GCCAGGAAGA GTGAGAAGTC TAAGAAGGAG 
    ATGGAGAAGA AGAAGAAGCA AGTGGCACGG ATAGAGGAGC AGCTGATGAA GCTGAAGTTA 
    CAGGCGACCG ACAAAGAGGA GAATAAGCAG ATTGCCCTGG GCACGTCCAA ACTCAACTAT 
    CTGGATCCCA GGATCACCGT AGCCTGGTGC AAGAAGTACG GCGTGCCCAT CGAGAAGATC 
    TATAACAAGA CGCAGAGAGA GAAATTTGCG TGGGCGATAG ACATGACCAG CGAGAACTTT 
    GAGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004919924.1
    CDS77..1885
    Translation

    Target ORF information:

    RefSeq Version XM_004919867.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004919867.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTGTACC GAGGCTGCAG GATTCTCCTT ACACTTAGTG CCGGGACTAG ATGGGCCCCC 
    CGTGTGTGGC CTCAACTGAG GGGGGTGTGC ATGGCAAGCA CTCGGGCATC TCCTGCCGTG 
    TTTAGGTGGG AGGAGGAGCA CTACGAGGAT GGCATGAAGT GGAAATTCCT GGAACACAGG 
    GGCCCATATT TTGCCCCTCC GTATGAGCCC CTCCCGGATG ATGTCAGCTT CTACTATGAT 
    GGGAAGCCAG TGAAACTGAG CCCAGGGGCC GAGGAAGTGG CCACTTTCTA TGGCAAAATG 
    CTGGACCATG AGTATACTAC CAAGGAGGCG TTCCAGAAGA ACTTCTTTGC AGACTGGAGA 
    GAGCAAATGA CGAATGAGGA GCGGAAGCTG ATCAAGCACC TACACAAGTG TGAATTCTCC 
    GAGATCCACA AGTATTTCTT TGAAAAGAAT GAGGCCCGCA AGGCTTTGCC CAAGGAAGAG 
    AAACAGAAAC TGAAGGAGGA AACCAATAAG ATCCAGGAGG AGTACGGCTA CTGCATCCTT 
    GATGGACACA GGGAAAAAAT TGGAAACTTC AAGATCGAGC CCCCAGGACT GTTCCGAGGC 
    CGTGGGGACC ACCCAAAGAT GGGAATGCTG AAGCTGAGGA TAATGCCAGA GGATGTGATT 
    ATAAACTGCA GCAAAGAATC CAAGGTTCCA GACCCCCCTC CGGGCCACAA GTGGAAAGAG 
    GTTCGGTGCG ACAACACCGT GACATGGCTG GCATCATGGA CGGAAAATAT CCAGAGTTCT 
    ATTAAGTACA TCATGCTGAA CCCAAGCTCC AAACTGAAGG GGGAGAAAGA CTGGCAGAAG 
    TACGAGACTG CTCGCCGACT GAAAGGGGTT GTGAGCGACA TCAGAGCTCA GTACCGAGTA 
    GACTGGAAAT CCCGGGAGAT GAAGAAACGG CAGAGAGCTG TGGCCCTCTA TTTCATTGAT 
    AAGCTGGCAC TCCGAGCGGG AAATGAAAAA GAGGAAGGGG AGACGGCAGA TACAGTTGGC 
    TGCTGTTCCC TTAGGGTGGA GCACATACAG CTGTACCCAG AGCTCGATGG GCAGCAGTAC 
    GTGGTGGAGT TTGACTTCCT GGGAAAGGAT TCCATCCGCT ATTATAACAA GGTGCCAGTG 
    GAGAAGGAGG TGTTTAAGAA CCTGAAGCTT TTCATGGAGA ATAAAAACCC GGAGGATGAT 
    CTTTTCGACA GGCTGAATAC TTCCATACTG AACAAACATC TACAGGATCT GATGGATGGA 
    CTTACGGCCA AAGTGTTCCG AACCTACAAT GCTTCTATCA CCCTGCAGAA CCAGCTGAAG 
    GAGCTGACCA ATGGGGAGGA CAATGTAGCG GCCAAGATTC TGTCTTATAA TCGGGCAAAC 
    AGAGCTGTAG CTCTTCTCTG TAACCACCAG AGGGCGCCAC CAAAAACCTT TGAAAAGTCA 
    ATGCAGAATC TACAGACTAA GATTGATGCA AAGGAACAGC AGCTGAAAGA AGCAAAGAAA 
    GAGTTAAAGA GCGCAAAGGC AGAGCATAAA GCCAGGAAGA GTGAGAAGTC TAAGAAGGAG 
    ATGGAGAAGA AGAAGAAGCA AGTGGCACGG ATAGAGGAGC AGCTGATGAA GCTGAAGTTA 
    CAGGCGACCG ACAAAGAGGA GAATAAGCAG ATTGCCCTGG GCACGTCCAA ACTCAACTAT 
    CTGGATCCCA GGATCACCGT AGCCTGGTGC AAGAAGTACG GCGTGCCCAT CGAGAAGATC 
    TATAACAAGA CGCAGAGAGA GAAATTTGCG TGGGCGATAG ACATGACCAG CGAGAACTTT 
    GAGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    top1mt ( XM_031903848.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759708.1 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa19269
    Clone ID Related Accession (Same CDS sequence) XM_031903848.1 , XM_004919865.3
    Accession Version XM_031903848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase I, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGGTATTT 
    CCTTCTCTTA CCATTGGTCT GTCTGCAGTC ACGTTTATAC ATCAAGCGCT TATGTACCCA 
    GAGCCGGAGA GGGGCCACAA GGAGAAGGGC CACAGTAAAT CTGAACCTGT GAAGCTGAAC 
    AGAAGCAGTC TAGTCAGGGA TGAGACCGTA TCTTCTACCA ACCCCACTGC TGAGGAGCCT 
    CCCAGGAGGA GGAAGCACAG ACTGGAGAAG GATGAAAAGG CCAAGTCTGC CGAAAGTTCC 
    GCTCATATAA AGAAAATCAA ATCTCACAAT CACCACACAG AAAGTGATAC TGGAGAGCCC 
    CTACCCAAGC ACAAAAAGGC AGACAATTCC CTCACAGAAA AGCTGCTGCA GGACGGTGTG 
    ATCTCCAGCG CCGCAGAAAA TGGGTTCTAT AAGTCAGTGG AACCGAGTGA TGGCAGAAAG 
    GAGATGTCTT GTGAAGTCAC CACCCGACTG GATGAGAACA GCAACATCAC CGCAATGGAG 
    TGCAATGGGG AATTTACACT TTACAGAGAG GATAAGGGGG TAGAATCTCC GTGCCAAACC 
    AGGAAAAATG AACCTAACTG GGAGAGTTCT ACTGCTGAAC CCAATGCTGG AGAAGAGACG 
    AGAAGAGAAG GACTCTGGGA GAGTACGGCA GATAGTAGGG CTACACTGGA CCCCCGCATA 
    AGCAAAGATA ACAGAAATAT ACAGGAACAA AAAGGGGGGT TGCCATGCAA AACTGAAAAG 
    GAAAGAGACG GCCATCACAA ATCCAAAGTA AAAAAGGATA ACGTTGATAG GTCTGAGAGG 
    AAAAAGGATC ATGTGGAGAA AAGTGGGGAT TCAGAGAAAA AGGAAAAGGG GGATTCAGAG 
    AAAAAGGAAA GGGGGAAAAA CCATTTGTGT AGAGATTCAG AGGGGGGGAA AGAAAGTTTA 
    AAAAGGGGCA AAGAAAACAA AGAGTCTAAA AGCGAGCAGA AAGCCATTGA TGGGACTGAA 
    GGCATCGCCC AGCCAGAAAG AGAGAAGAAA TGTGAAGATA AAATGGCATC AGGGAAAGAG 
    AGAGAATCTG CCGAGGAGAA GGAAACTGGT AAGAGAGACC CCCTGAAATC CAAAGATAAA 
    ACATCTGCTG GGGAGGAAAG AAAGCCAAAA AAGCATTCTC ACAAAGAGAA GTCCAAAGAG 
    ATGAACAGAA CAAGAGAGAA GATGGAAGAT GCTAAGATGT CAGTGTCTCG TGACCATCAC 
    AGGAAAGGAG ATGCCCTGGA GGGGGCAGTG GAAGCCAACG AGGCAGTAAA ACCTGAAAGA 
    ATTGAGGTGG CTGTTGGGGA ACCCAAGGAA CACAGCAGGG AAGTAGATGA GAAGGTCCAT 
    CTCAGGGTAA AGAGCTTCGA GGAGGAGCTG AATGAGTTGG CCAACCTGAA AATGCAGAGC 
    AGCAAAGTGA AGAAGAAGGA GAGGGAAACG AAGAAGCACG AGGAGAAGAG AGAGAAGATA 
    AAGGAGCAAA GCAGCGATGG GGCTGAAGGT AAAGCTACCA AAAGGGAAGG TGAGGAAGAG 
    GAGTTGGTGG GTGAGGAGGC CAAACCTAGG GTAGAAAAGA ATCAGAAAGA GAAGATTAAA 
    GAGAGAGGAA AAAAAAGCTC CAAGGATGAG GCCGCTAGAG ACAAAAAGCA GCCTTCTCGG 
    AAACGCAAAA GCACTGAAAC CGAAGGGACG GAAGCCAAAA AGGCAAAAGA TGCGGCCAAA 
    AAGGAGGAAG AATCAAAGTG GAAATGGTGG GAGGAGGAGC ACTACGAGGA TGGCATGAAG 
    TGGAAATTCC TGGAACACAG GGGCCCATAT TTTGCCCCTC CGTATGAGCC CCTCCCGGAT 
    GATGTCAGCT TCTACTATGA TGGGAAGCCA GTGAAACTGA GCCCAGGGGC CGAGGAAGTG 
    GCCACTTTCT ATGGCAAAAT GCTGGACCAT GAGTATACTA CCAAGGAGGC GTTCCAGAAG 
    AACTTCTTTG CAGACTGGAG AGAGCAAATG ACGAATGAGG AGCGGAAGCT GATCAAGCAC 
    CTACACAAGT GTGAATTCTC CGAGATCCAC AAGTATTTCT TTGAAAAGAA TGAGGCCCGC 
    AAGGCTTTGC CCAAGGAAGA GAAACAGAAA CTGAAGGAGG AAACCAATAA GATCCAGGAG 
    GAGTACGGCT ACTGCATCCT TGATGGACAC AGGGAAAAAA TTGGAAACTT CAAGATCGAG 
    CCCCCAGGAC TGTTCCGAGG CCGTGGGGAC CACCCAAAGA TGGGAATGCT GAAGCTGAGG 
    ATAATGCCAG AGGATGTGAT TATAAACTGC AGCAAAGAAT CCAAGGTTCC AGACCCCCCT 
    CCGGGCCACA AGTGGAAAGA GGTTCGGTGC GACAACACCG TGACATGGCT GGCATCATGG 
    ACGGAAAATA TCCAGAGTTC TATTAAGTAC ATCATGCTGA ACCCAAGCTC CAAACTGAAG 
    GGGGAGAAAG ACTGGCAGAA GTACGAGACT GCTCGCCGAC TGAAAGGGGT TGTGAGCGAC 
    ATCAGAGCTC AGTACCGAGT AGACTGGAAA TCCCGGGAGA TGAAGAAACG GCAGAGAGCT 
    GTGGCCCTCT ATTTCATTGA TAAGCTGGCA CTCCGAGCGG GAAATGAAAA AGAGGAAGGG 
    GAGACGGCAG ATACAGTTGG CTGCTGTTCC CTTAGGGTGG AGCACATACA GCTGTACCCA 
    GAGCTCGATG GGCAGCAGTA CGTGGTGGAG TTTGACTTCC TGGGAAAGGA TTCCATCCGC 
    TATTATAACA AGGTGCCAGT GGAGAAGGAG GTGTTTAAGA ACCTGAAGCT TTTCATGGAG 
    AATAAAAACC CGGAGGATGA TCTTTTCGAC AGGCTGAATA CTTCCATACT GAACAAACAT 
    CTACAGGATC TGATGGATGG ACTTACGGCC AAAGTGTTCC GAACCTACAA TGCTTCTATC 
    ACCCTGCAGA ACCAGCTGAA GGAGCTGACC AATGGGGAGG ACAATGTAGC GGCCAAGATT 
    CTGTCTTATA ATCGGGCAAA CAGAGCTGTA GCTCTTCTCT GTAACCACCA GAGGGCGCCA 
    CCAAAAACCT TTGAAAAGTC AATGCAGAAT CTACAGACTA AGATTGATGC AAAGGAACAG 
    CAGCTGAAAG AAGCAAAGAA AGAGTTAAAG AGCGCAAAGG CAGAGCATAA AGCCAGGAAG 
    AGTGAGAAGT CTAAGAAGGA GATGGAGAAG AAGAAGAAGC AAGTGGCACG GATAGAGGAG 
    CAGCTGATGA AGCTGAAGTT ACAGGCGACC GACAAAGAGG AGAATAAGCA GATTGCCCTG 
    GGCACGTCCA AACTCAACTA TCTGGATCCC AGGATCACCG TAGCCTGGTG CAAGAAGTAC 
    GGCGTGCCCA TCGAGAAGAT CTATAACAAG ACGCAGAGAG AGAAATTTGC GTGGGCGATA 
    GACATGACCA GCGAGAACTT TGAGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759708.1
    CDS124..3573
    Translation

    Target ORF information:

    RefSeq Version XM_031903848.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031903848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGGTATTT 
    CCTTCTCTTA CCATTGGTCT GTCTGCAGTC ACGTTTATAC ATCAAGCGCT TATGTACCCA 
    GAGCCGGAGA GGGGCCACAA GGAGAAGGGC CACAGTAAAT CTGAACCTGT GAAGCTGAAC 
    AGAAGCAGTC TAGTCAGGGA TGAGACCGTA TCTTCTACCA ACCCCACTGC TGAGGAGCCT 
    CCCAGGAGGA GGAAGCACAG ACTGGAGAAG GATGAAAAGG CCAAGTCTGC CGAAAGTTCC 
    GCTCATATAA AGAAAATCAA ATCTCACAAT CACCACACAG AAAGTGATAC TGGAGAGCCC 
    CTACCCAAGC ACAAAAAGGC AGACAATTCC CTCACAGAAA AGCTGCTGCA GGACGGTGTG 
    ATCTCCAGCG CCGCAGAAAA TGGGTTCTAT AAGTCAGTGG AACCGAGTGA TGGCAGAAAG 
    GAGATGTCTT GTGAAGTCAC CACCCGACTG GATGAGAACA GCAACATCAC CGCAATGGAG 
    TGCAATGGGG AATTTACACT TTACAGAGAG GATAAGGGGG TAGAATCTCC GTGCCAAACC 
    AGGAAAAATG AACCTAACTG GGAGAGTTCT ACTGCTGAAC CCAATGCTGG AGAAGAGACG 
    AGAAGAGAAG GACTCTGGGA GAGTACGGCA GATAGTAGGG CTACACTGGA CCCCCGCATA 
    AGCAAAGATA ACAGAAATAT ACAGGAACAA AAAGGGGGGT TGCCATGCAA AACTGAAAAG 
    GAAAGAGACG GCCATCACAA ATCCAAAGTA AAAAAGGATA ACGTTGATAG GTCTGAGAGG 
    AAAAAGGATC ATGTGGAGAA AAGTGGGGAT TCAGAGAAAA AGGAAAAGGG GGATTCAGAG 
    AAAAAGGAAA GGGGGAAAAA CCATTTGTGT AGAGATTCAG AGGGGGGGAA AGAAAGTTTA 
    AAAAGGGGCA AAGAAAACAA AGAGTCTAAA AGCGAGCAGA AAGCCATTGA TGGGACTGAA 
    GGCATCGCCC AGCCAGAAAG AGAGAAGAAA TGTGAAGATA AAATGGCATC AGGGAAAGAG 
    AGAGAATCTG CCGAGGAGAA GGAAACTGGT AAGAGAGACC CCCTGAAATC CAAAGATAAA 
    ACATCTGCTG GGGAGGAAAG AAAGCCAAAA AAGCATTCTC ACAAAGAGAA GTCCAAAGAG 
    ATGAACAGAA CAAGAGAGAA GATGGAAGAT GCTAAGATGT CAGTGTCTCG TGACCATCAC 
    AGGAAAGGAG ATGCCCTGGA GGGGGCAGTG GAAGCCAACG AGGCAGTAAA ACCTGAAAGA 
    ATTGAGGTGG CTGTTGGGGA ACCCAAGGAA CACAGCAGGG AAGTAGATGA GAAGGTCCAT 
    CTCAGGGTAA AGAGCTTCGA GGAGGAGCTG AATGAGTTGG CCAACCTGAA AATGCAGAGC 
    AGCAAAGTGA AGAAGAAGGA GAGGGAAACG AAGAAGCACG AGGAGAAGAG AGAGAAGATA 
    AAGGAGCAAA GCAGCGATGG GGCTGAAGGT AAAGCTACCA AAAGGGAAGG TGAGGAAGAG 
    GAGTTGGTGG GTGAGGAGGC CAAACCTAGG GTAGAAAAGA ATCAGAAAGA GAAGATTAAA 
    GAGAGAGGAA AAAAAAGCTC CAAGGATGAG GCCGCTAGAG ACAAAAAGCA GCCTTCTCGG 
    AAACGCAAAA GCACTGAAAC CGAAGGGACG GAAGCCAAAA AGGCAAAAGA TGCGGCCAAA 
    AAGGAGGAAG AATCAAAGTG GAAATGGTGG GAGGAGGAGC ACTACGAGGA TGGCATGAAG 
    TGGAAATTCC TGGAACACAG GGGCCCATAT TTTGCCCCTC CGTATGAGCC CCTCCCGGAT 
    GATGTCAGCT TCTACTATGA TGGGAAGCCA GTGAAACTGA GCCCAGGGGC CGAGGAAGTG 
    GCCACTTTCT ATGGCAAAAT GCTGGACCAT GAGTATACTA CCAAGGAGGC GTTCCAGAAG 
    AACTTCTTTG CAGACTGGAG AGAGCAAATG ACGAATGAGG AGCGGAAGCT GATCAAGCAC 
    CTACACAAGT GTGAATTCTC CGAGATCCAC AAGTATTTCT TTGAAAAGAA TGAGGCCCGC 
    AAGGCTTTGC CCAAGGAAGA GAAACAGAAA CTGAAGGAGG AAACCAATAA GATCCAGGAG 
    GAGTACGGCT ACTGCATCCT TGATGGACAC AGGGAAAAAA TTGGAAACTT CAAGATCGAG 
    CCCCCAGGAC TGTTCCGAGG CCGTGGGGAC CACCCAAAGA TGGGAATGCT GAAGCTGAGG 
    ATAATGCCAG AGGATGTGAT TATAAACTGC AGCAAAGAAT CCAAGGTTCC AGACCCCCCT 
    CCGGGCCACA AGTGGAAAGA GGTTCGGTGC GACAACACCG TGACATGGCT GGCATCATGG 
    ACGGAAAATA TCCAGAGTTC TATTAAGTAC ATCATGCTGA ACCCAAGCTC CAAACTGAAG 
    GGGGAGAAAG ACTGGCAGAA GTACGAGACT GCTCGCCGAC TGAAAGGGGT TGTGAGCGAC 
    ATCAGAGCTC AGTACCGAGT AGACTGGAAA TCCCGGGAGA TGAAGAAACG GCAGAGAGCT 
    GTGGCCCTCT ATTTCATTGA TAAGCTGGCA CTCCGAGCGG GAAATGAAAA AGAGGAAGGG 
    GAGACGGCAG ATACAGTTGG CTGCTGTTCC CTTAGGGTGG AGCACATACA GCTGTACCCA 
    GAGCTCGATG GGCAGCAGTA CGTGGTGGAG TTTGACTTCC TGGGAAAGGA TTCCATCCGC 
    TATTATAACA AGGTGCCAGT GGAGAAGGAG GTGTTTAAGA ACCTGAAGCT TTTCATGGAG 
    AATAAAAACC CGGAGGATGA TCTTTTCGAC AGGCTGAATA CTTCCATACT GAACAAACAT 
    CTACAGGATC TGATGGATGG ACTTACGGCC AAAGTGTTCC GAACCTACAA TGCTTCTATC 
    ACCCTGCAGA ACCAGCTGAA GGAGCTGACC AATGGGGAGG ACAATGTAGC GGCCAAGATT 
    CTGTCTTATA ATCGGGCAAA CAGAGCTGTA GCTCTTCTCT GTAACCACCA GAGGGCGCCA 
    CCAAAAACCT TTGAAAAGTC AATGCAGAAT CTACAGACTA AGATTGATGC AAAGGAACAG 
    CAGCTGAAAG AAGCAAAGAA AGAGTTAAAG AGCGCAAAGG CAGAGCATAA AGCCAGGAAG 
    AGTGAGAAGT CTAAGAAGGA GATGGAGAAG AAGAAGAAGC AAGTGGCACG GATAGAGGAG 
    CAGCTGATGA AGCTGAAGTT ACAGGCGACC GACAAAGAGG AGAATAAGCA GATTGCCCTG 
    GGCACGTCCA AACTCAACTA TCTGGATCCC AGGATCACCG TAGCCTGGTG CAAGAAGTAC 
    GGCGTGCCCA TCGAGAAGAT CTATAACAAG ACGCAGAGAG AGAAATTTGC GTGGGCGATA 
    GACATGACCA GCGAGAACTT TGAGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    top1mt ( XM_031903850.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759710.1 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OXa19270
    Clone ID Related Accession (Same CDS sequence) XM_004919866.3 , XM_031903850.1 , XM_031903851.1 , XM_031903852.1
    Accession Version XM_031903850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase I, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGAGGGGC 
    CACAAGGAGA AGGGCCACAG TAAATCTGAA CCTGTGAAGC TGAACAGAAG CAGTCTAGTC 
    AGGGATGAGA CCGTATCTTC TACCAACCCC ACTGCTGAGG AGCCTCCCAG GAGGAGGAAG 
    CACAGACTGG AGAAGGATGA AAAGGCCAAG TCTGCCGAAA GTTCCGCTCA TATAAAGAAA 
    ATCAAATCTC ACAATCACCA CACAGAAAGT GATACTGGAG AGCCCCTACC CAAGCACAAA 
    AAGGCAGACA ATTCCCTCAC AGAAAAGCTG CTGCAGGACG GTGTGATCTC CAGCGCCGCA 
    GAAAATGGGT TCTATAAGTC AGTGGAACCG AGTGATGGCA GAAAGGAGAT GTCTTGTGAA 
    GTCACCACCC GACTGGATGA GAACAGCAAC ATCACCGCAA TGGAGTGCAA TGGGGAATTT 
    ACACTTTACA GAGAGGATAA GGGGGTAGAA TCTCCGTGCC AAACCAGGAA AAATGAACCT 
    AACTGGGAGA GTTCTACTGC TGAACCCAAT GCTGGAGAAG AGACGAGAAG AGAAGGACTC 
    TGGGAGAGTA CGGCAGATAG TAGGGCTACA CTGGACCCCC GCATAAGCAA AGATAACAGA 
    AATATACAGG AACAAAAAGG GGGGTTGCCA TGCAAAACTG AAAAGGAAAG AGACGGCCAT 
    CACAAATCCA AAGTAAAAAA GGATAACGTT GATAGGTCTG AGAGGAAAAA GGATCATGTG 
    GAGAAAAGTG GGGATTCAGA GAAAAAGGAA AAGGGGGATT CAGAGAAAAA GGAAAGGGGG 
    AAAAACCATT TGTGTAGAGA TTCAGAGGGG GGGAAAGAAA GTTTAAAAAG GGGCAAAGAA 
    AACAAAGAGT CTAAAAGCGA GCAGAAAGCC ATTGATGGGA CTGAAGGCAT CGCCCAGCCA 
    GAAAGAGAGA AGAAATGTGA AGATAAAATG GCATCAGGGA AAGAGAGAGA ATCTGCCGAG 
    GAGAAGGAAA CTGGTAAGAG AGACCCCCTG AAATCCAAAG ATAAAACATC TGCTGGGGAG 
    GAAAGAAAGC CAAAAAAGCA TTCTCACAAA GAGAAGTCCA AAGAGATGAA CAGAACAAGA 
    GAGAAGATGG AAGATGCTAA GATGTCAGTG TCTCGTGACC ATCACAGGAA AGGAGATGCC 
    CTGGAGGGGG CAGTGGAAGC CAACGAGGCA GTAAAACCTG AAAGAATTGA GGTGGCTGTT 
    GGGGAACCCA AGGAACACAG CAGGGAAGTA GATGAGAAGG TCCATCTCAG GGTAAAGAGC 
    TTCGAGGAGG AGCTGAATGA GTTGGCCAAC CTGAAAATGC AGAGCAGCAA AGTGAAGAAG 
    AAGGAGAGGG AAACGAAGAA GCACGAGGAG AAGAGAGAGA AGATAAAGGA GCAAAGCAGC 
    GATGGGGCTG AAGGTAAAGC TACCAAAAGG GAAGGTGAGG AAGAGGAGTT GGTGGGTGAG 
    GAGGCCAAAC CTAGGGTAGA AAAGAATCAG AAAGAGAAGA TTAAAGAGAG AGGAAAAAAA 
    AGCTCCAAGG ATGAGGCCGC TAGAGACAAA AAGCAGCCTT CTCGGAAACG CAAAAGCACT 
    GAAACCGAAG GGACGGAAGC CAAAAAGGCA AAAGATGCGG CCAAAAAGGA GGAAGAATCA 
    AAGTGGAAAT GGTGGGAGGA GGAGCACTAC GAGGATGGCA TGAAGTGGAA ATTCCTGGAA 
    CACAGGGGCC CATATTTTGC CCCTCCGTAT GAGCCCCTCC CGGATGATGT CAGCTTCTAC 
    TATGATGGGA AGCCAGTGAA ACTGAGCCCA GGGGCCGAGG AAGTGGCCAC TTTCTATGGC 
    AAAATGCTGG ACCATGAGTA TACTACCAAG GAGGCGTTCC AGAAGAACTT CTTTGCAGAC 
    TGGAGAGAGC AAATGACGAA TGAGGAGCGG AAGCTGATCA AGCACCTACA CAAGTGTGAA 
    TTCTCCGAGA TCCACAAGTA TTTCTTTGAA AAGAATGAGG CCCGCAAGGC TTTGCCCAAG 
    GAAGAGAAAC AGAAACTGAA GGAGGAAACC AATAAGATCC AGGAGGAGTA CGGCTACTGC 
    ATCCTTGATG GACACAGGGA AAAAATTGGA AACTTCAAGA TCGAGCCCCC AGGACTGTTC 
    CGAGGCCGTG GGGACCACCC AAAGATGGGA ATGCTGAAGC TGAGGATAAT GCCAGAGGAT 
    GTGATTATAA ACTGCAGCAA AGAATCCAAG GTTCCAGACC CCCCTCCGGG CCACAAGTGG 
    AAAGAGGTTC GGTGCGACAA CACCGTGACA TGGCTGGCAT CATGGACGGA AAATATCCAG 
    AGTTCTATTA AGTACATCAT GCTGAACCCA AGCTCCAAAC TGAAGGGGGA GAAAGACTGG 
    CAGAAGTACG AGACTGCTCG CCGACTGAAA GGGGTTGTGA GCGACATCAG AGCTCAGTAC 
    CGAGTAGACT GGAAATCCCG GGAGATGAAG AAACGGCAGA GAGCTGTGGC CCTCTATTTC 
    ATTGATAAGC TGGCACTCCG AGCGGGAAAT GAAAAAGAGG AAGGGGAGAC GGCAGATACA 
    GTTGGCTGCT GTTCCCTTAG GGTGGAGCAC ATACAGCTGT ACCCAGAGCT CGATGGGCAG 
    CAGTACGTGG TGGAGTTTGA CTTCCTGGGA AAGGATTCCA TCCGCTATTA TAACAAGGTG 
    CCAGTGGAGA AGGAGGTGTT TAAGAACCTG AAGCTTTTCA TGGAGAATAA AAACCCGGAG 
    GATGATCTTT TCGACAGGCT GAATACTTCC ATACTGAACA AACATCTACA GGATCTGATG 
    GATGGACTTA CGGCCAAAGT GTTCCGAACC TACAATGCTT CTATCACCCT GCAGAACCAG 
    CTGAAGGAGC TGACCAATGG GGAGGACAAT GTAGCGGCCA AGATTCTGTC TTATAATCGG 
    GCAAACAGAG CTGTAGCTCT TCTCTGTAAC CACCAGAGGG CGCCACCAAA AACCTTTGAA 
    AAGTCAATGC AGAATCTACA GACTAAGATT GATGCAAAGG AACAGCAGCT GAAAGAAGCA 
    AAGAAAGAGT TAAAGAGCGC AAAGGCAGAG CATAAAGCCA GGAAGAGTGA GAAGTCTAAG 
    AAGGAGATGG AGAAGAAGAA GAAGCAAGTG GCACGGATAG AGGAGCAGCT GATGAAGCTG 
    AAGTTACAGG CGACCGACAA AGAGGAGAAT AAGCAGATTG CCCTGGGCAC GTCCAAACTC 
    AACTATCTGG ATCCCAGGAT CACCGTAGCC TGGTGCAAGA AGTACGGCGT GCCCATCGAG 
    AAGATCTATA ACAAGACGCA GAGAGAGAAA TTTGCGTGGG CGATAGACAT GACCAGCGAG 
    AACTTTGAGT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759710.1
    CDS126..3500
    Translation

    Target ORF information:

    RefSeq Version XM_031903850.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031903850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGAGGGGC 
    CACAAGGAGA AGGGCCACAG TAAATCTGAA CCTGTGAAGC TGAACAGAAG CAGTCTAGTC 
    AGGGATGAGA CCGTATCTTC TACCAACCCC ACTGCTGAGG AGCCTCCCAG GAGGAGGAAG 
    CACAGACTGG AGAAGGATGA AAAGGCCAAG TCTGCCGAAA GTTCCGCTCA TATAAAGAAA 
    ATCAAATCTC ACAATCACCA CACAGAAAGT GATACTGGAG AGCCCCTACC CAAGCACAAA 
    AAGGCAGACA ATTCCCTCAC AGAAAAGCTG CTGCAGGACG GTGTGATCTC CAGCGCCGCA 
    GAAAATGGGT TCTATAAGTC AGTGGAACCG AGTGATGGCA GAAAGGAGAT GTCTTGTGAA 
    GTCACCACCC GACTGGATGA GAACAGCAAC ATCACCGCAA TGGAGTGCAA TGGGGAATTT 
    ACACTTTACA GAGAGGATAA GGGGGTAGAA TCTCCGTGCC AAACCAGGAA AAATGAACCT 
    AACTGGGAGA GTTCTACTGC TGAACCCAAT GCTGGAGAAG AGACGAGAAG AGAAGGACTC 
    TGGGAGAGTA CGGCAGATAG TAGGGCTACA CTGGACCCCC GCATAAGCAA AGATAACAGA 
    AATATACAGG AACAAAAAGG GGGGTTGCCA TGCAAAACTG AAAAGGAAAG AGACGGCCAT 
    CACAAATCCA AAGTAAAAAA GGATAACGTT GATAGGTCTG AGAGGAAAAA GGATCATGTG 
    GAGAAAAGTG GGGATTCAGA GAAAAAGGAA AAGGGGGATT CAGAGAAAAA GGAAAGGGGG 
    AAAAACCATT TGTGTAGAGA TTCAGAGGGG GGGAAAGAAA GTTTAAAAAG GGGCAAAGAA 
    AACAAAGAGT CTAAAAGCGA GCAGAAAGCC ATTGATGGGA CTGAAGGCAT CGCCCAGCCA 
    GAAAGAGAGA AGAAATGTGA AGATAAAATG GCATCAGGGA AAGAGAGAGA ATCTGCCGAG 
    GAGAAGGAAA CTGGTAAGAG AGACCCCCTG AAATCCAAAG ATAAAACATC TGCTGGGGAG 
    GAAAGAAAGC CAAAAAAGCA TTCTCACAAA GAGAAGTCCA AAGAGATGAA CAGAACAAGA 
    GAGAAGATGG AAGATGCTAA GATGTCAGTG TCTCGTGACC ATCACAGGAA AGGAGATGCC 
    CTGGAGGGGG CAGTGGAAGC CAACGAGGCA GTAAAACCTG AAAGAATTGA GGTGGCTGTT 
    GGGGAACCCA AGGAACACAG CAGGGAAGTA GATGAGAAGG TCCATCTCAG GGTAAAGAGC 
    TTCGAGGAGG AGCTGAATGA GTTGGCCAAC CTGAAAATGC AGAGCAGCAA AGTGAAGAAG 
    AAGGAGAGGG AAACGAAGAA GCACGAGGAG AAGAGAGAGA AGATAAAGGA GCAAAGCAGC 
    GATGGGGCTG AAGGTAAAGC TACCAAAAGG GAAGGTGAGG AAGAGGAGTT GGTGGGTGAG 
    GAGGCCAAAC CTAGGGTAGA AAAGAATCAG AAAGAGAAGA TTAAAGAGAG AGGAAAAAAA 
    AGCTCCAAGG ATGAGGCCGC TAGAGACAAA AAGCAGCCTT CTCGGAAACG CAAAAGCACT 
    GAAACCGAAG GGACGGAAGC CAAAAAGGCA AAAGATGCGG CCAAAAAGGA GGAAGAATCA 
    AAGTGGAAAT GGTGGGAGGA GGAGCACTAC GAGGATGGCA TGAAGTGGAA ATTCCTGGAA 
    CACAGGGGCC CATATTTTGC CCCTCCGTAT GAGCCCCTCC CGGATGATGT CAGCTTCTAC 
    TATGATGGGA AGCCAGTGAA ACTGAGCCCA GGGGCCGAGG AAGTGGCCAC TTTCTATGGC 
    AAAATGCTGG ACCATGAGTA TACTACCAAG GAGGCGTTCC AGAAGAACTT CTTTGCAGAC 
    TGGAGAGAGC AAATGACGAA TGAGGAGCGG AAGCTGATCA AGCACCTACA CAAGTGTGAA 
    TTCTCCGAGA TCCACAAGTA TTTCTTTGAA AAGAATGAGG CCCGCAAGGC TTTGCCCAAG 
    GAAGAGAAAC AGAAACTGAA GGAGGAAACC AATAAGATCC AGGAGGAGTA CGGCTACTGC 
    ATCCTTGATG GACACAGGGA AAAAATTGGA AACTTCAAGA TCGAGCCCCC AGGACTGTTC 
    CGAGGCCGTG GGGACCACCC AAAGATGGGA ATGCTGAAGC TGAGGATAAT GCCAGAGGAT 
    GTGATTATAA ACTGCAGCAA AGAATCCAAG GTTCCAGACC CCCCTCCGGG CCACAAGTGG 
    AAAGAGGTTC GGTGCGACAA CACCGTGACA TGGCTGGCAT CATGGACGGA AAATATCCAG 
    AGTTCTATTA AGTACATCAT GCTGAACCCA AGCTCCAAAC TGAAGGGGGA GAAAGACTGG 
    CAGAAGTACG AGACTGCTCG CCGACTGAAA GGGGTTGTGA GCGACATCAG AGCTCAGTAC 
    CGAGTAGACT GGAAATCCCG GGAGATGAAG AAACGGCAGA GAGCTGTGGC CCTCTATTTC 
    ATTGATAAGC TGGCACTCCG AGCGGGAAAT GAAAAAGAGG AAGGGGAGAC GGCAGATACA 
    GTTGGCTGCT GTTCCCTTAG GGTGGAGCAC ATACAGCTGT ACCCAGAGCT CGATGGGCAG 
    CAGTACGTGG TGGAGTTTGA CTTCCTGGGA AAGGATTCCA TCCGCTATTA TAACAAGGTG 
    CCAGTGGAGA AGGAGGTGTT TAAGAACCTG AAGCTTTTCA TGGAGAATAA AAACCCGGAG 
    GATGATCTTT TCGACAGGCT GAATACTTCC ATACTGAACA AACATCTACA GGATCTGATG 
    GATGGACTTA CGGCCAAAGT GTTCCGAACC TACAATGCTT CTATCACCCT GCAGAACCAG 
    CTGAAGGAGC TGACCAATGG GGAGGACAAT GTAGCGGCCA AGATTCTGTC TTATAATCGG 
    GCAAACAGAG CTGTAGCTCT TCTCTGTAAC CACCAGAGGG CGCCACCAAA AACCTTTGAA 
    AAGTCAATGC AGAATCTACA GACTAAGATT GATGCAAAGG AACAGCAGCT GAAAGAAGCA 
    AAGAAAGAGT TAAAGAGCGC AAAGGCAGAG CATAAAGCCA GGAAGAGTGA GAAGTCTAAG 
    AAGGAGATGG AGAAGAAGAA GAAGCAAGTG GCACGGATAG AGGAGCAGCT GATGAAGCTG 
    AAGTTACAGG CGACCGACAA AGAGGAGAAT AAGCAGATTG CCCTGGGCAC GTCCAAACTC 
    AACTATCTGG ATCCCAGGAT CACCGTAGCC TGGTGCAAGA AGTACGGCGT GCCCATCGAG 
    AAGATCTATA ACAAGACGCA GAGAGAGAAA TTTGCGTGGG CGATAGACAT GACCAGCGAG 
    AACTTTGAGT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    top1mt ( XM_031903851.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759711.1 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OXa19270
    Clone ID Related Accession (Same CDS sequence) XM_004919866.3 , XM_031903850.1 , XM_031903851.1 , XM_031903852.1
    Accession Version XM_031903851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase I, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGAGGGGC 
    CACAAGGAGA AGGGCCACAG TAAATCTGAA CCTGTGAAGC TGAACAGAAG CAGTCTAGTC 
    AGGGATGAGA CCGTATCTTC TACCAACCCC ACTGCTGAGG AGCCTCCCAG GAGGAGGAAG 
    CACAGACTGG AGAAGGATGA AAAGGCCAAG TCTGCCGAAA GTTCCGCTCA TATAAAGAAA 
    ATCAAATCTC ACAATCACCA CACAGAAAGT GATACTGGAG AGCCCCTACC CAAGCACAAA 
    AAGGCAGACA ATTCCCTCAC AGAAAAGCTG CTGCAGGACG GTGTGATCTC CAGCGCCGCA 
    GAAAATGGGT TCTATAAGTC AGTGGAACCG AGTGATGGCA GAAAGGAGAT GTCTTGTGAA 
    GTCACCACCC GACTGGATGA GAACAGCAAC ATCACCGCAA TGGAGTGCAA TGGGGAATTT 
    ACACTTTACA GAGAGGATAA GGGGGTAGAA TCTCCGTGCC AAACCAGGAA AAATGAACCT 
    AACTGGGAGA GTTCTACTGC TGAACCCAAT GCTGGAGAAG AGACGAGAAG AGAAGGACTC 
    TGGGAGAGTA CGGCAGATAG TAGGGCTACA CTGGACCCCC GCATAAGCAA AGATAACAGA 
    AATATACAGG AACAAAAAGG GGGGTTGCCA TGCAAAACTG AAAAGGAAAG AGACGGCCAT 
    CACAAATCCA AAGTAAAAAA GGATAACGTT GATAGGTCTG AGAGGAAAAA GGATCATGTG 
    GAGAAAAGTG GGGATTCAGA GAAAAAGGAA AAGGGGGATT CAGAGAAAAA GGAAAGGGGG 
    AAAAACCATT TGTGTAGAGA TTCAGAGGGG GGGAAAGAAA GTTTAAAAAG GGGCAAAGAA 
    AACAAAGAGT CTAAAAGCGA GCAGAAAGCC ATTGATGGGA CTGAAGGCAT CGCCCAGCCA 
    GAAAGAGAGA AGAAATGTGA AGATAAAATG GCATCAGGGA AAGAGAGAGA ATCTGCCGAG 
    GAGAAGGAAA CTGGTAAGAG AGACCCCCTG AAATCCAAAG ATAAAACATC TGCTGGGGAG 
    GAAAGAAAGC CAAAAAAGCA TTCTCACAAA GAGAAGTCCA AAGAGATGAA CAGAACAAGA 
    GAGAAGATGG AAGATGCTAA GATGTCAGTG TCTCGTGACC ATCACAGGAA AGGAGATGCC 
    CTGGAGGGGG CAGTGGAAGC CAACGAGGCA GTAAAACCTG AAAGAATTGA GGTGGCTGTT 
    GGGGAACCCA AGGAACACAG CAGGGAAGTA GATGAGAAGG TCCATCTCAG GGTAAAGAGC 
    TTCGAGGAGG AGCTGAATGA GTTGGCCAAC CTGAAAATGC AGAGCAGCAA AGTGAAGAAG 
    AAGGAGAGGG AAACGAAGAA GCACGAGGAG AAGAGAGAGA AGATAAAGGA GCAAAGCAGC 
    GATGGGGCTG AAGGTAAAGC TACCAAAAGG GAAGGTGAGG AAGAGGAGTT GGTGGGTGAG 
    GAGGCCAAAC CTAGGGTAGA AAAGAATCAG AAAGAGAAGA TTAAAGAGAG AGGAAAAAAA 
    AGCTCCAAGG ATGAGGCCGC TAGAGACAAA AAGCAGCCTT CTCGGAAACG CAAAAGCACT 
    GAAACCGAAG GGACGGAAGC CAAAAAGGCA AAAGATGCGG CCAAAAAGGA GGAAGAATCA 
    AAGTGGAAAT GGTGGGAGGA GGAGCACTAC GAGGATGGCA TGAAGTGGAA ATTCCTGGAA 
    CACAGGGGCC CATATTTTGC CCCTCCGTAT GAGCCCCTCC CGGATGATGT CAGCTTCTAC 
    TATGATGGGA AGCCAGTGAA ACTGAGCCCA GGGGCCGAGG AAGTGGCCAC TTTCTATGGC 
    AAAATGCTGG ACCATGAGTA TACTACCAAG GAGGCGTTCC AGAAGAACTT CTTTGCAGAC 
    TGGAGAGAGC AAATGACGAA TGAGGAGCGG AAGCTGATCA AGCACCTACA CAAGTGTGAA 
    TTCTCCGAGA TCCACAAGTA TTTCTTTGAA AAGAATGAGG CCCGCAAGGC TTTGCCCAAG 
    GAAGAGAAAC AGAAACTGAA GGAGGAAACC AATAAGATCC AGGAGGAGTA CGGCTACTGC 
    ATCCTTGATG GACACAGGGA AAAAATTGGA AACTTCAAGA TCGAGCCCCC AGGACTGTTC 
    CGAGGCCGTG GGGACCACCC AAAGATGGGA ATGCTGAAGC TGAGGATAAT GCCAGAGGAT 
    GTGATTATAA ACTGCAGCAA AGAATCCAAG GTTCCAGACC CCCCTCCGGG CCACAAGTGG 
    AAAGAGGTTC GGTGCGACAA CACCGTGACA TGGCTGGCAT CATGGACGGA AAATATCCAG 
    AGTTCTATTA AGTACATCAT GCTGAACCCA AGCTCCAAAC TGAAGGGGGA GAAAGACTGG 
    CAGAAGTACG AGACTGCTCG CCGACTGAAA GGGGTTGTGA GCGACATCAG AGCTCAGTAC 
    CGAGTAGACT GGAAATCCCG GGAGATGAAG AAACGGCAGA GAGCTGTGGC CCTCTATTTC 
    ATTGATAAGC TGGCACTCCG AGCGGGAAAT GAAAAAGAGG AAGGGGAGAC GGCAGATACA 
    GTTGGCTGCT GTTCCCTTAG GGTGGAGCAC ATACAGCTGT ACCCAGAGCT CGATGGGCAG 
    CAGTACGTGG TGGAGTTTGA CTTCCTGGGA AAGGATTCCA TCCGCTATTA TAACAAGGTG 
    CCAGTGGAGA AGGAGGTGTT TAAGAACCTG AAGCTTTTCA TGGAGAATAA AAACCCGGAG 
    GATGATCTTT TCGACAGGCT GAATACTTCC ATACTGAACA AACATCTACA GGATCTGATG 
    GATGGACTTA CGGCCAAAGT GTTCCGAACC TACAATGCTT CTATCACCCT GCAGAACCAG 
    CTGAAGGAGC TGACCAATGG GGAGGACAAT GTAGCGGCCA AGATTCTGTC TTATAATCGG 
    GCAAACAGAG CTGTAGCTCT TCTCTGTAAC CACCAGAGGG CGCCACCAAA AACCTTTGAA 
    AAGTCAATGC AGAATCTACA GACTAAGATT GATGCAAAGG AACAGCAGCT GAAAGAAGCA 
    AAGAAAGAGT TAAAGAGCGC AAAGGCAGAG CATAAAGCCA GGAAGAGTGA GAAGTCTAAG 
    AAGGAGATGG AGAAGAAGAA GAAGCAAGTG GCACGGATAG AGGAGCAGCT GATGAAGCTG 
    AAGTTACAGG CGACCGACAA AGAGGAGAAT AAGCAGATTG CCCTGGGCAC GTCCAAACTC 
    AACTATCTGG ATCCCAGGAT CACCGTAGCC TGGTGCAAGA AGTACGGCGT GCCCATCGAG 
    AAGATCTATA ACAAGACGCA GAGAGAGAAA TTTGCGTGGG CGATAGACAT GACCAGCGAG 
    AACTTTGAGT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759711.1
    CDS111..3485
    Translation

    Target ORF information:

    RefSeq Version XM_031903851.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031903851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGAGGGGC 
    CACAAGGAGA AGGGCCACAG TAAATCTGAA CCTGTGAAGC TGAACAGAAG CAGTCTAGTC 
    AGGGATGAGA CCGTATCTTC TACCAACCCC ACTGCTGAGG AGCCTCCCAG GAGGAGGAAG 
    CACAGACTGG AGAAGGATGA AAAGGCCAAG TCTGCCGAAA GTTCCGCTCA TATAAAGAAA 
    ATCAAATCTC ACAATCACCA CACAGAAAGT GATACTGGAG AGCCCCTACC CAAGCACAAA 
    AAGGCAGACA ATTCCCTCAC AGAAAAGCTG CTGCAGGACG GTGTGATCTC CAGCGCCGCA 
    GAAAATGGGT TCTATAAGTC AGTGGAACCG AGTGATGGCA GAAAGGAGAT GTCTTGTGAA 
    GTCACCACCC GACTGGATGA GAACAGCAAC ATCACCGCAA TGGAGTGCAA TGGGGAATTT 
    ACACTTTACA GAGAGGATAA GGGGGTAGAA TCTCCGTGCC AAACCAGGAA AAATGAACCT 
    AACTGGGAGA GTTCTACTGC TGAACCCAAT GCTGGAGAAG AGACGAGAAG AGAAGGACTC 
    TGGGAGAGTA CGGCAGATAG TAGGGCTACA CTGGACCCCC GCATAAGCAA AGATAACAGA 
    AATATACAGG AACAAAAAGG GGGGTTGCCA TGCAAAACTG AAAAGGAAAG AGACGGCCAT 
    CACAAATCCA AAGTAAAAAA GGATAACGTT GATAGGTCTG AGAGGAAAAA GGATCATGTG 
    GAGAAAAGTG GGGATTCAGA GAAAAAGGAA AAGGGGGATT CAGAGAAAAA GGAAAGGGGG 
    AAAAACCATT TGTGTAGAGA TTCAGAGGGG GGGAAAGAAA GTTTAAAAAG GGGCAAAGAA 
    AACAAAGAGT CTAAAAGCGA GCAGAAAGCC ATTGATGGGA CTGAAGGCAT CGCCCAGCCA 
    GAAAGAGAGA AGAAATGTGA AGATAAAATG GCATCAGGGA AAGAGAGAGA ATCTGCCGAG 
    GAGAAGGAAA CTGGTAAGAG AGACCCCCTG AAATCCAAAG ATAAAACATC TGCTGGGGAG 
    GAAAGAAAGC CAAAAAAGCA TTCTCACAAA GAGAAGTCCA AAGAGATGAA CAGAACAAGA 
    GAGAAGATGG AAGATGCTAA GATGTCAGTG TCTCGTGACC ATCACAGGAA AGGAGATGCC 
    CTGGAGGGGG CAGTGGAAGC CAACGAGGCA GTAAAACCTG AAAGAATTGA GGTGGCTGTT 
    GGGGAACCCA AGGAACACAG CAGGGAAGTA GATGAGAAGG TCCATCTCAG GGTAAAGAGC 
    TTCGAGGAGG AGCTGAATGA GTTGGCCAAC CTGAAAATGC AGAGCAGCAA AGTGAAGAAG 
    AAGGAGAGGG AAACGAAGAA GCACGAGGAG AAGAGAGAGA AGATAAAGGA GCAAAGCAGC 
    GATGGGGCTG AAGGTAAAGC TACCAAAAGG GAAGGTGAGG AAGAGGAGTT GGTGGGTGAG 
    GAGGCCAAAC CTAGGGTAGA AAAGAATCAG AAAGAGAAGA TTAAAGAGAG AGGAAAAAAA 
    AGCTCCAAGG ATGAGGCCGC TAGAGACAAA AAGCAGCCTT CTCGGAAACG CAAAAGCACT 
    GAAACCGAAG GGACGGAAGC CAAAAAGGCA AAAGATGCGG CCAAAAAGGA GGAAGAATCA 
    AAGTGGAAAT GGTGGGAGGA GGAGCACTAC GAGGATGGCA TGAAGTGGAA ATTCCTGGAA 
    CACAGGGGCC CATATTTTGC CCCTCCGTAT GAGCCCCTCC CGGATGATGT CAGCTTCTAC 
    TATGATGGGA AGCCAGTGAA ACTGAGCCCA GGGGCCGAGG AAGTGGCCAC TTTCTATGGC 
    AAAATGCTGG ACCATGAGTA TACTACCAAG GAGGCGTTCC AGAAGAACTT CTTTGCAGAC 
    TGGAGAGAGC AAATGACGAA TGAGGAGCGG AAGCTGATCA AGCACCTACA CAAGTGTGAA 
    TTCTCCGAGA TCCACAAGTA TTTCTTTGAA AAGAATGAGG CCCGCAAGGC TTTGCCCAAG 
    GAAGAGAAAC AGAAACTGAA GGAGGAAACC AATAAGATCC AGGAGGAGTA CGGCTACTGC 
    ATCCTTGATG GACACAGGGA AAAAATTGGA AACTTCAAGA TCGAGCCCCC AGGACTGTTC 
    CGAGGCCGTG GGGACCACCC AAAGATGGGA ATGCTGAAGC TGAGGATAAT GCCAGAGGAT 
    GTGATTATAA ACTGCAGCAA AGAATCCAAG GTTCCAGACC CCCCTCCGGG CCACAAGTGG 
    AAAGAGGTTC GGTGCGACAA CACCGTGACA TGGCTGGCAT CATGGACGGA AAATATCCAG 
    AGTTCTATTA AGTACATCAT GCTGAACCCA AGCTCCAAAC TGAAGGGGGA GAAAGACTGG 
    CAGAAGTACG AGACTGCTCG CCGACTGAAA GGGGTTGTGA GCGACATCAG AGCTCAGTAC 
    CGAGTAGACT GGAAATCCCG GGAGATGAAG AAACGGCAGA GAGCTGTGGC CCTCTATTTC 
    ATTGATAAGC TGGCACTCCG AGCGGGAAAT GAAAAAGAGG AAGGGGAGAC GGCAGATACA 
    GTTGGCTGCT GTTCCCTTAG GGTGGAGCAC ATACAGCTGT ACCCAGAGCT CGATGGGCAG 
    CAGTACGTGG TGGAGTTTGA CTTCCTGGGA AAGGATTCCA TCCGCTATTA TAACAAGGTG 
    CCAGTGGAGA AGGAGGTGTT TAAGAACCTG AAGCTTTTCA TGGAGAATAA AAACCCGGAG 
    GATGATCTTT TCGACAGGCT GAATACTTCC ATACTGAACA AACATCTACA GGATCTGATG 
    GATGGACTTA CGGCCAAAGT GTTCCGAACC TACAATGCTT CTATCACCCT GCAGAACCAG 
    CTGAAGGAGC TGACCAATGG GGAGGACAAT GTAGCGGCCA AGATTCTGTC TTATAATCGG 
    GCAAACAGAG CTGTAGCTCT TCTCTGTAAC CACCAGAGGG CGCCACCAAA AACCTTTGAA 
    AAGTCAATGC AGAATCTACA GACTAAGATT GATGCAAAGG AACAGCAGCT GAAAGAAGCA 
    AAGAAAGAGT TAAAGAGCGC AAAGGCAGAG CATAAAGCCA GGAAGAGTGA GAAGTCTAAG 
    AAGGAGATGG AGAAGAAGAA GAAGCAAGTG GCACGGATAG AGGAGCAGCT GATGAAGCTG 
    AAGTTACAGG CGACCGACAA AGAGGAGAAT AAGCAGATTG CCCTGGGCAC GTCCAAACTC 
    AACTATCTGG ATCCCAGGAT CACCGTAGCC TGGTGCAAGA AGTACGGCGT GCCCATCGAG 
    AAGATCTATA ACAAGACGCA GAGAGAGAAA TTTGCGTGGG CGATAGACAT GACCAGCGAG 
    AACTTTGAGT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    top1mt ( XM_031903852.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759712.1 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OXa19270
    Clone ID Related Accession (Same CDS sequence) XM_004919866.3 , XM_031903850.1 , XM_031903851.1 , XM_031903852.1
    Accession Version XM_031903852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase I, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGAGGGGC 
    CACAAGGAGA AGGGCCACAG TAAATCTGAA CCTGTGAAGC TGAACAGAAG CAGTCTAGTC 
    AGGGATGAGA CCGTATCTTC TACCAACCCC ACTGCTGAGG AGCCTCCCAG GAGGAGGAAG 
    CACAGACTGG AGAAGGATGA AAAGGCCAAG TCTGCCGAAA GTTCCGCTCA TATAAAGAAA 
    ATCAAATCTC ACAATCACCA CACAGAAAGT GATACTGGAG AGCCCCTACC CAAGCACAAA 
    AAGGCAGACA ATTCCCTCAC AGAAAAGCTG CTGCAGGACG GTGTGATCTC CAGCGCCGCA 
    GAAAATGGGT TCTATAAGTC AGTGGAACCG AGTGATGGCA GAAAGGAGAT GTCTTGTGAA 
    GTCACCACCC GACTGGATGA GAACAGCAAC ATCACCGCAA TGGAGTGCAA TGGGGAATTT 
    ACACTTTACA GAGAGGATAA GGGGGTAGAA TCTCCGTGCC AAACCAGGAA AAATGAACCT 
    AACTGGGAGA GTTCTACTGC TGAACCCAAT GCTGGAGAAG AGACGAGAAG AGAAGGACTC 
    TGGGAGAGTA CGGCAGATAG TAGGGCTACA CTGGACCCCC GCATAAGCAA AGATAACAGA 
    AATATACAGG AACAAAAAGG GGGGTTGCCA TGCAAAACTG AAAAGGAAAG AGACGGCCAT 
    CACAAATCCA AAGTAAAAAA GGATAACGTT GATAGGTCTG AGAGGAAAAA GGATCATGTG 
    GAGAAAAGTG GGGATTCAGA GAAAAAGGAA AAGGGGGATT CAGAGAAAAA GGAAAGGGGG 
    AAAAACCATT TGTGTAGAGA TTCAGAGGGG GGGAAAGAAA GTTTAAAAAG GGGCAAAGAA 
    AACAAAGAGT CTAAAAGCGA GCAGAAAGCC ATTGATGGGA CTGAAGGCAT CGCCCAGCCA 
    GAAAGAGAGA AGAAATGTGA AGATAAAATG GCATCAGGGA AAGAGAGAGA ATCTGCCGAG 
    GAGAAGGAAA CTGGTAAGAG AGACCCCCTG AAATCCAAAG ATAAAACATC TGCTGGGGAG 
    GAAAGAAAGC CAAAAAAGCA TTCTCACAAA GAGAAGTCCA AAGAGATGAA CAGAACAAGA 
    GAGAAGATGG AAGATGCTAA GATGTCAGTG TCTCGTGACC ATCACAGGAA AGGAGATGCC 
    CTGGAGGGGG CAGTGGAAGC CAACGAGGCA GTAAAACCTG AAAGAATTGA GGTGGCTGTT 
    GGGGAACCCA AGGAACACAG CAGGGAAGTA GATGAGAAGG TCCATCTCAG GGTAAAGAGC 
    TTCGAGGAGG AGCTGAATGA GTTGGCCAAC CTGAAAATGC AGAGCAGCAA AGTGAAGAAG 
    AAGGAGAGGG AAACGAAGAA GCACGAGGAG AAGAGAGAGA AGATAAAGGA GCAAAGCAGC 
    GATGGGGCTG AAGGTAAAGC TACCAAAAGG GAAGGTGAGG AAGAGGAGTT GGTGGGTGAG 
    GAGGCCAAAC CTAGGGTAGA AAAGAATCAG AAAGAGAAGA TTAAAGAGAG AGGAAAAAAA 
    AGCTCCAAGG ATGAGGCCGC TAGAGACAAA AAGCAGCCTT CTCGGAAACG CAAAAGCACT 
    GAAACCGAAG GGACGGAAGC CAAAAAGGCA AAAGATGCGG CCAAAAAGGA GGAAGAATCA 
    AAGTGGAAAT GGTGGGAGGA GGAGCACTAC GAGGATGGCA TGAAGTGGAA ATTCCTGGAA 
    CACAGGGGCC CATATTTTGC CCCTCCGTAT GAGCCCCTCC CGGATGATGT CAGCTTCTAC 
    TATGATGGGA AGCCAGTGAA ACTGAGCCCA GGGGCCGAGG AAGTGGCCAC TTTCTATGGC 
    AAAATGCTGG ACCATGAGTA TACTACCAAG GAGGCGTTCC AGAAGAACTT CTTTGCAGAC 
    TGGAGAGAGC AAATGACGAA TGAGGAGCGG AAGCTGATCA AGCACCTACA CAAGTGTGAA 
    TTCTCCGAGA TCCACAAGTA TTTCTTTGAA AAGAATGAGG CCCGCAAGGC TTTGCCCAAG 
    GAAGAGAAAC AGAAACTGAA GGAGGAAACC AATAAGATCC AGGAGGAGTA CGGCTACTGC 
    ATCCTTGATG GACACAGGGA AAAAATTGGA AACTTCAAGA TCGAGCCCCC AGGACTGTTC 
    CGAGGCCGTG GGGACCACCC AAAGATGGGA ATGCTGAAGC TGAGGATAAT GCCAGAGGAT 
    GTGATTATAA ACTGCAGCAA AGAATCCAAG GTTCCAGACC CCCCTCCGGG CCACAAGTGG 
    AAAGAGGTTC GGTGCGACAA CACCGTGACA TGGCTGGCAT CATGGACGGA AAATATCCAG 
    AGTTCTATTA AGTACATCAT GCTGAACCCA AGCTCCAAAC TGAAGGGGGA GAAAGACTGG 
    CAGAAGTACG AGACTGCTCG CCGACTGAAA GGGGTTGTGA GCGACATCAG AGCTCAGTAC 
    CGAGTAGACT GGAAATCCCG GGAGATGAAG AAACGGCAGA GAGCTGTGGC CCTCTATTTC 
    ATTGATAAGC TGGCACTCCG AGCGGGAAAT GAAAAAGAGG AAGGGGAGAC GGCAGATACA 
    GTTGGCTGCT GTTCCCTTAG GGTGGAGCAC ATACAGCTGT ACCCAGAGCT CGATGGGCAG 
    CAGTACGTGG TGGAGTTTGA CTTCCTGGGA AAGGATTCCA TCCGCTATTA TAACAAGGTG 
    CCAGTGGAGA AGGAGGTGTT TAAGAACCTG AAGCTTTTCA TGGAGAATAA AAACCCGGAG 
    GATGATCTTT TCGACAGGCT GAATACTTCC ATACTGAACA AACATCTACA GGATCTGATG 
    GATGGACTTA CGGCCAAAGT GTTCCGAACC TACAATGCTT CTATCACCCT GCAGAACCAG 
    CTGAAGGAGC TGACCAATGG GGAGGACAAT GTAGCGGCCA AGATTCTGTC TTATAATCGG 
    GCAAACAGAG CTGTAGCTCT TCTCTGTAAC CACCAGAGGG CGCCACCAAA AACCTTTGAA 
    AAGTCAATGC AGAATCTACA GACTAAGATT GATGCAAAGG AACAGCAGCT GAAAGAAGCA 
    AAGAAAGAGT TAAAGAGCGC AAAGGCAGAG CATAAAGCCA GGAAGAGTGA GAAGTCTAAG 
    AAGGAGATGG AGAAGAAGAA GAAGCAAGTG GCACGGATAG AGGAGCAGCT GATGAAGCTG 
    AAGTTACAGG CGACCGACAA AGAGGAGAAT AAGCAGATTG CCCTGGGCAC GTCCAAACTC 
    AACTATCTGG ATCCCAGGAT CACCGTAGCC TGGTGCAAGA AGTACGGCGT GCCCATCGAG 
    AAGATCTATA ACAAGACGCA GAGAGAGAAA TTTGCGTGGG CGATAGACAT GACCAGCGAG 
    AACTTTGAGT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759712.1
    CDS43..3417
    Translation

    Target ORF information:

    RefSeq Version XM_031903852.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031903852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGAGGGGC 
    CACAAGGAGA AGGGCCACAG TAAATCTGAA CCTGTGAAGC TGAACAGAAG CAGTCTAGTC 
    AGGGATGAGA CCGTATCTTC TACCAACCCC ACTGCTGAGG AGCCTCCCAG GAGGAGGAAG 
    CACAGACTGG AGAAGGATGA AAAGGCCAAG TCTGCCGAAA GTTCCGCTCA TATAAAGAAA 
    ATCAAATCTC ACAATCACCA CACAGAAAGT GATACTGGAG AGCCCCTACC CAAGCACAAA 
    AAGGCAGACA ATTCCCTCAC AGAAAAGCTG CTGCAGGACG GTGTGATCTC CAGCGCCGCA 
    GAAAATGGGT TCTATAAGTC AGTGGAACCG AGTGATGGCA GAAAGGAGAT GTCTTGTGAA 
    GTCACCACCC GACTGGATGA GAACAGCAAC ATCACCGCAA TGGAGTGCAA TGGGGAATTT 
    ACACTTTACA GAGAGGATAA GGGGGTAGAA TCTCCGTGCC AAACCAGGAA AAATGAACCT 
    AACTGGGAGA GTTCTACTGC TGAACCCAAT GCTGGAGAAG AGACGAGAAG AGAAGGACTC 
    TGGGAGAGTA CGGCAGATAG TAGGGCTACA CTGGACCCCC GCATAAGCAA AGATAACAGA 
    AATATACAGG AACAAAAAGG GGGGTTGCCA TGCAAAACTG AAAAGGAAAG AGACGGCCAT 
    CACAAATCCA AAGTAAAAAA GGATAACGTT GATAGGTCTG AGAGGAAAAA GGATCATGTG 
    GAGAAAAGTG GGGATTCAGA GAAAAAGGAA AAGGGGGATT CAGAGAAAAA GGAAAGGGGG 
    AAAAACCATT TGTGTAGAGA TTCAGAGGGG GGGAAAGAAA GTTTAAAAAG GGGCAAAGAA 
    AACAAAGAGT CTAAAAGCGA GCAGAAAGCC ATTGATGGGA CTGAAGGCAT CGCCCAGCCA 
    GAAAGAGAGA AGAAATGTGA AGATAAAATG GCATCAGGGA AAGAGAGAGA ATCTGCCGAG 
    GAGAAGGAAA CTGGTAAGAG AGACCCCCTG AAATCCAAAG ATAAAACATC TGCTGGGGAG 
    GAAAGAAAGC CAAAAAAGCA TTCTCACAAA GAGAAGTCCA AAGAGATGAA CAGAACAAGA 
    GAGAAGATGG AAGATGCTAA GATGTCAGTG TCTCGTGACC ATCACAGGAA AGGAGATGCC 
    CTGGAGGGGG CAGTGGAAGC CAACGAGGCA GTAAAACCTG AAAGAATTGA GGTGGCTGTT 
    GGGGAACCCA AGGAACACAG CAGGGAAGTA GATGAGAAGG TCCATCTCAG GGTAAAGAGC 
    TTCGAGGAGG AGCTGAATGA GTTGGCCAAC CTGAAAATGC AGAGCAGCAA AGTGAAGAAG 
    AAGGAGAGGG AAACGAAGAA GCACGAGGAG AAGAGAGAGA AGATAAAGGA GCAAAGCAGC 
    GATGGGGCTG AAGGTAAAGC TACCAAAAGG GAAGGTGAGG AAGAGGAGTT GGTGGGTGAG 
    GAGGCCAAAC CTAGGGTAGA AAAGAATCAG AAAGAGAAGA TTAAAGAGAG AGGAAAAAAA 
    AGCTCCAAGG ATGAGGCCGC TAGAGACAAA AAGCAGCCTT CTCGGAAACG CAAAAGCACT 
    GAAACCGAAG GGACGGAAGC CAAAAAGGCA AAAGATGCGG CCAAAAAGGA GGAAGAATCA 
    AAGTGGAAAT GGTGGGAGGA GGAGCACTAC GAGGATGGCA TGAAGTGGAA ATTCCTGGAA 
    CACAGGGGCC CATATTTTGC CCCTCCGTAT GAGCCCCTCC CGGATGATGT CAGCTTCTAC 
    TATGATGGGA AGCCAGTGAA ACTGAGCCCA GGGGCCGAGG AAGTGGCCAC TTTCTATGGC 
    AAAATGCTGG ACCATGAGTA TACTACCAAG GAGGCGTTCC AGAAGAACTT CTTTGCAGAC 
    TGGAGAGAGC AAATGACGAA TGAGGAGCGG AAGCTGATCA AGCACCTACA CAAGTGTGAA 
    TTCTCCGAGA TCCACAAGTA TTTCTTTGAA AAGAATGAGG CCCGCAAGGC TTTGCCCAAG 
    GAAGAGAAAC AGAAACTGAA GGAGGAAACC AATAAGATCC AGGAGGAGTA CGGCTACTGC 
    ATCCTTGATG GACACAGGGA AAAAATTGGA AACTTCAAGA TCGAGCCCCC AGGACTGTTC 
    CGAGGCCGTG GGGACCACCC AAAGATGGGA ATGCTGAAGC TGAGGATAAT GCCAGAGGAT 
    GTGATTATAA ACTGCAGCAA AGAATCCAAG GTTCCAGACC CCCCTCCGGG CCACAAGTGG 
    AAAGAGGTTC GGTGCGACAA CACCGTGACA TGGCTGGCAT CATGGACGGA AAATATCCAG 
    AGTTCTATTA AGTACATCAT GCTGAACCCA AGCTCCAAAC TGAAGGGGGA GAAAGACTGG 
    CAGAAGTACG AGACTGCTCG CCGACTGAAA GGGGTTGTGA GCGACATCAG AGCTCAGTAC 
    CGAGTAGACT GGAAATCCCG GGAGATGAAG AAACGGCAGA GAGCTGTGGC CCTCTATTTC 
    ATTGATAAGC TGGCACTCCG AGCGGGAAAT GAAAAAGAGG AAGGGGAGAC GGCAGATACA 
    GTTGGCTGCT GTTCCCTTAG GGTGGAGCAC ATACAGCTGT ACCCAGAGCT CGATGGGCAG 
    CAGTACGTGG TGGAGTTTGA CTTCCTGGGA AAGGATTCCA TCCGCTATTA TAACAAGGTG 
    CCAGTGGAGA AGGAGGTGTT TAAGAACCTG AAGCTTTTCA TGGAGAATAA AAACCCGGAG 
    GATGATCTTT TCGACAGGCT GAATACTTCC ATACTGAACA AACATCTACA GGATCTGATG 
    GATGGACTTA CGGCCAAAGT GTTCCGAACC TACAATGCTT CTATCACCCT GCAGAACCAG 
    CTGAAGGAGC TGACCAATGG GGAGGACAAT GTAGCGGCCA AGATTCTGTC TTATAATCGG 
    GCAAACAGAG CTGTAGCTCT TCTCTGTAAC CACCAGAGGG CGCCACCAAA AACCTTTGAA 
    AAGTCAATGC AGAATCTACA GACTAAGATT GATGCAAAGG AACAGCAGCT GAAAGAAGCA 
    AAGAAAGAGT TAAAGAGCGC AAAGGCAGAG CATAAAGCCA GGAAGAGTGA GAAGTCTAAG 
    AAGGAGATGG AGAAGAAGAA GAAGCAAGTG GCACGGATAG AGGAGCAGCT GATGAAGCTG 
    AAGTTACAGG CGACCGACAA AGAGGAGAAT AAGCAGATTG CCCTGGGCAC GTCCAAACTC 
    AACTATCTGG ATCCCAGGAT CACCGTAGCC TGGTGCAAGA AGTACGGCGT GCCCATCGAG 
    AAGATCTATA ACAAGACGCA GAGAGAGAAA TTTGCGTGGG CGATAGACAT GACCAGCGAG 
    AACTTTGAGT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    top1mt ( XM_031903849.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759709.1 Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OXa60242
    Clone ID Related Accession (Same CDS sequence) XM_031903849.1
    Accession Version XM_031903849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase I, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGGTATTT 
    CCTTCTCTTA CCATTGGTCT GTCTGCAGTC ACGTTTATAC ATCAAGCGCT TATGTACCCA 
    GAGCCGGAGA GGGGCCACAA GGAGAAGGGC CACAGTAAAT CTGAACCTGT GAAGCTGAAC 
    AGAAGCAGTC TAGTCAGGGA TGAGACCGTA TCTTCTACCA ACCCCACTGC TGAGGAGCCT 
    CCCAGGAGGA GGAAGCACAG ACTGGAGAAG GATGAAAAGG CCAAGTCTGC CGAAAGTTCC 
    GCTCATATAA AGAAAATCAA ATCTCACAAT CACCACACAG AAAGTGATAC TGGAGAGCCC 
    CTACCCAAGC ACAAAAAGGC AGACAATTCC CTCACAGAAA AGCTGCTGCA GGACGGTGTG 
    ATCTCCAGCG CCGCAGAAAA TGGGTTCTAT AAGTCAGTGG AACCGAGTGA TGGCAGAAAG 
    GAGATGTCTT GTGAAGTCAC CACCCGACTG GATGAGAACA GCAACATCAC CGCAATGGAG 
    TGCAATGGGG AATTTACACT TTACAGAGAG GATAAGGGGG TAGAATCTCC GTGCCAAACC 
    AGGAAAAATG AACCTAACTG GGAGAGTTCT ACTGCTGAAC CCAATGCTGG AGAAGAGACG 
    AGAAGAGAAG GACTCTGGGA GAGTACGGCA GATAGTAGGG CTACACTGGA CCCCCGCATA 
    AGCAAAGATA ACAGAAATAT ACAGGAACAA AAAGGGGGGT TGCCATGCAA AACTGAAAAG 
    GAAAGAGACG GCCATCACAA ATCCAAAGTA AAAAAGGATA ACGTTGATAG GTCTGAGAGG 
    AAAAAGGATC ATGTGGAGAA AAGTGGGGAT TCAGAGAAAA AGGAAAAGGG GGATTCAGAG 
    AAAAAGGAAA GGGGGAAAAA CCATTTGTGT AGAGATTCAG AGGGGGGGAA AGAAAGTTTA 
    AAAAGGGGCA AAGAAAACAA AGAGTCTAAA AGCGAGCAGA AAGCCATTGA TGGGACTGAA 
    GGCATCGCCC AGCCAGAAAG AGAGAAGAAA TGTGAAGATA AAATGGCATC AGGGAAAGAG 
    AGAGAATCTG CCGAGGAGAA GGAAACTGGT AAGAGAGACC CCCTGAAATC CAAAGATAAA 
    ACATCTGCTG GGGAGGAAAG AAAGCCAAAA AAGCATTCTC ACAAAGAGAA GTCCAAAGAG 
    ATGAACAGAA CAAGAGAGAA GATGGAAGAT GCTAAGATGT CAGTGTCTCG TGACCATCAC 
    AGGAAAGGAG ATGCCCTGGA GGGGGCAGTG GAAGCCAACG AGGCAGTAAA ACCTGAAAGA 
    ATTGAGGTGG CTGTTGGGGA ACCCAAGGAA CACAGCAGGG AAGTAGATGA GAAGGTCCAT 
    CTCAGGGTAA AGAGCTTCGA GGAGGAGCTG AATGAGTTGG CCAACCTGAA AATGCAGAGC 
    AGCAAAGTGA AGAAGAAGGA GAGGGAAACG AAGAAGCACG AGGAGAAGAG AGAGAAGATA 
    AAGGAGCAAA GCAGCGATGG GGCTGAAGGT AAAGCTACCA AAAGGGAAGG TGAGGAAGAG 
    GAGTTGGTGG GTGAGGAGGC CAAACCTAGG GTAGAAAAGA ATCAGAAAGA GAAGATTAAA 
    GAGAGAGGAA AAAAAAGCTC CAAGGATGAG GCCGCTAGAG ACAAAAAGCA GCCTTCTCGG 
    AAACGCAAAA GCACTGAAAC CGAAGGGACG GAAGCCAAAA AGGCAAAAGA TGCGGCCAAA 
    AAGGAGGAAG AATCAAAGTG GAAATGGTGG GAGGAGGAGC ACTACGAGGA TGGCATGAAG 
    TGGAAATTCC TGGAACACAG GGGCCCATAT TTTGCCCCTC CGTATGAGCC CCTCCCGGAT 
    GATGTCAGCT TCTACTATGA TGGGAAGCCA GTGAAACTGA GCCCAGGGGC CGAGGAAGTG 
    GCCACTTTCT ATGGCAAAAT GCTGGACCAT GAGTATACTA CCAAGGAGGC GTTCCAGAAG 
    AACTTCTTTG CAGACTGGAG AGAGCAAATG ACGAATGAGG AGCGGAAGCT GATCAAGCAC 
    CTACACAAGT GTGAATTCTC CGAGATCCAC AAGTATTTCT TTGAAAAGAA TGAGGCCCGC 
    AAGGCTTTGC CCAAGGAAGA GAAACAGAAA CTGAAGGAGG AAACCAATAA GATCCAGGAG 
    GAGTACGGCT ACTGCATCCT TGATGGACAC AGGGAAAAAA TTGGAAACTT CAAGATCGAG 
    CCCCCAGGAC TGTTCCGAGG CCGTGGGGAC CACCCAAAGA TGGGAATGCT GAAGCTGAGG 
    ATAATGCCAG AGGATGTGAT TATAAACTGC AGCAAAGAAT CCAAGGTTCC AGACCCCCCT 
    CCGGGCCACA AGTGGAAAGA GGTTCGGTGC GACAACACCG TGACATGGCT GGCATCATGG 
    ACGGAAAATA TCCAGAGTTC TATTAAGTAC ATCATGCTGA ACCCAAGCTC CAAACTGAAG 
    GGGGAGAAAG ACTGGCAGAA GTACGAGACT GCTCGCCGAC TGAAAGGGGT TGTGAGCGAC 
    ATCAGAGCTC AGTACCGAGT AGACTGGAAA TCCCGGGAGA TGAAGAAACG GCAGAGAGCT 
    GTGGCCCTCT ATTTCATTGA TAAGCTGGCA CTCCGAGCGG GAAATGAAAA AGAGGAAGGG 
    GAGACGGCAG ATACAGTTGG CTGCTGTTCC CTTAGGGTGG AGCACATACA GCTGTACCCA 
    GAGCTCGATG GGCAGCAGTA CGTGGTGGAG TTTGACTTCC TGGGAAAGGA TTCCATCCGC 
    TATTATAACA AGGTGCCAGT GGAGAAGGAG GTGTTTAAGA ACCTGAAGCT TTTCATGGAG 
    AATAAAAACC CGGAGGATGA TCTTTTCGAC AGGCTGAATA CTTCCATACT GAACAAACAT 
    CTACAGGATC TGATGGATGG ACTTACGGCC AAAGTGTTCC GAACCTACAA TGCTTCTATC 
    ACCCTGCAGA ACCAGCTGAA GGAGCTGACC AATGGGGAGG ACAATGTAGC GGCCAAGATT 
    CTGTCTTATA ATCGGGCAAA CAGAGCTGTA GCTCTTCTCT GTAACCACCA GAGGGCGCCA 
    CCAAAAACCT TTGAAAAGTC AATGCAGAAT CTACAGACTA AGATTGATGC AAAGGAACAG 
    CAGCTGAAAG AAGCAAAGAA AGAGTTAAAG AGCGCAAAGG CAGAGCATAA AGCCAGGAAG 
    AGTGAGAAGT CTAAGAAGGA GATGGAGAAG AAGAAGAAGC AAGTGGCACG GATAGAGGAG 
    CAGCTGATGA AGCTGAAGTT ACAGGCGACC GACAAAGAGG AGAATAAGCA GATTGCCCTG 
    GGCACGTCCA AACTCAACTA TCTGGATCCC AGGATCACCG TAGCCTGTTC TCTGTTTGGT 
    CTGCCCCAGG TGCAAGAAGT ACGGCGTGCC CATCGAGAAG ATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759709.1
    CDS125..3532
    Translation

    Target ORF information:

    RefSeq Version XM_031903849.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase I mitochondrial (top1mt), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031903849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGGTATTT 
    CCTTCTCTTA CCATTGGTCT GTCTGCAGTC ACGTTTATAC ATCAAGCGCT TATGTACCCA 
    GAGCCGGAGA GGGGCCACAA GGAGAAGGGC CACAGTAAAT CTGAACCTGT GAAGCTGAAC 
    AGAAGCAGTC TAGTCAGGGA TGAGACCGTA TCTTCTACCA ACCCCACTGC TGAGGAGCCT 
    CCCAGGAGGA GGAAGCACAG ACTGGAGAAG GATGAAAAGG CCAAGTCTGC CGAAAGTTCC 
    GCTCATATAA AGAAAATCAA ATCTCACAAT CACCACACAG AAAGTGATAC TGGAGAGCCC 
    CTACCCAAGC ACAAAAAGGC AGACAATTCC CTCACAGAAA AGCTGCTGCA GGACGGTGTG 
    ATCTCCAGCG CCGCAGAAAA TGGGTTCTAT AAGTCAGTGG AACCGAGTGA TGGCAGAAAG 
    GAGATGTCTT GTGAAGTCAC CACCCGACTG GATGAGAACA GCAACATCAC CGCAATGGAG 
    TGCAATGGGG AATTTACACT TTACAGAGAG GATAAGGGGG TAGAATCTCC GTGCCAAACC 
    AGGAAAAATG AACCTAACTG GGAGAGTTCT ACTGCTGAAC CCAATGCTGG AGAAGAGACG 
    AGAAGAGAAG GACTCTGGGA GAGTACGGCA GATAGTAGGG CTACACTGGA CCCCCGCATA 
    AGCAAAGATA ACAGAAATAT ACAGGAACAA AAAGGGGGGT TGCCATGCAA AACTGAAAAG 
    GAAAGAGACG GCCATCACAA ATCCAAAGTA AAAAAGGATA ACGTTGATAG GTCTGAGAGG 
    AAAAAGGATC ATGTGGAGAA AAGTGGGGAT TCAGAGAAAA AGGAAAAGGG GGATTCAGAG 
    AAAAAGGAAA GGGGGAAAAA CCATTTGTGT AGAGATTCAG AGGGGGGGAA AGAAAGTTTA 
    AAAAGGGGCA AAGAAAACAA AGAGTCTAAA AGCGAGCAGA AAGCCATTGA TGGGACTGAA 
    GGCATCGCCC AGCCAGAAAG AGAGAAGAAA TGTGAAGATA AAATGGCATC AGGGAAAGAG 
    AGAGAATCTG CCGAGGAGAA GGAAACTGGT AAGAGAGACC CCCTGAAATC CAAAGATAAA 
    ACATCTGCTG GGGAGGAAAG AAAGCCAAAA AAGCATTCTC ACAAAGAGAA GTCCAAAGAG 
    ATGAACAGAA CAAGAGAGAA GATGGAAGAT GCTAAGATGT CAGTGTCTCG TGACCATCAC 
    AGGAAAGGAG ATGCCCTGGA GGGGGCAGTG GAAGCCAACG AGGCAGTAAA ACCTGAAAGA 
    ATTGAGGTGG CTGTTGGGGA ACCCAAGGAA CACAGCAGGG AAGTAGATGA GAAGGTCCAT 
    CTCAGGGTAA AGAGCTTCGA GGAGGAGCTG AATGAGTTGG CCAACCTGAA AATGCAGAGC 
    AGCAAAGTGA AGAAGAAGGA GAGGGAAACG AAGAAGCACG AGGAGAAGAG AGAGAAGATA 
    AAGGAGCAAA GCAGCGATGG GGCTGAAGGT AAAGCTACCA AAAGGGAAGG TGAGGAAGAG 
    GAGTTGGTGG GTGAGGAGGC CAAACCTAGG GTAGAAAAGA ATCAGAAAGA GAAGATTAAA 
    GAGAGAGGAA AAAAAAGCTC CAAGGATGAG GCCGCTAGAG ACAAAAAGCA GCCTTCTCGG 
    AAACGCAAAA GCACTGAAAC CGAAGGGACG GAAGCCAAAA AGGCAAAAGA TGCGGCCAAA 
    AAGGAGGAAG AATCAAAGTG GAAATGGTGG GAGGAGGAGC ACTACGAGGA TGGCATGAAG 
    TGGAAATTCC TGGAACACAG GGGCCCATAT TTTGCCCCTC CGTATGAGCC CCTCCCGGAT 
    GATGTCAGCT TCTACTATGA TGGGAAGCCA GTGAAACTGA GCCCAGGGGC CGAGGAAGTG 
    GCCACTTTCT ATGGCAAAAT GCTGGACCAT GAGTATACTA CCAAGGAGGC GTTCCAGAAG 
    AACTTCTTTG CAGACTGGAG AGAGCAAATG ACGAATGAGG AGCGGAAGCT GATCAAGCAC 
    CTACACAAGT GTGAATTCTC CGAGATCCAC AAGTATTTCT TTGAAAAGAA TGAGGCCCGC 
    AAGGCTTTGC CCAAGGAAGA GAAACAGAAA CTGAAGGAGG AAACCAATAA GATCCAGGAG 
    GAGTACGGCT ACTGCATCCT TGATGGACAC AGGGAAAAAA TTGGAAACTT CAAGATCGAG 
    CCCCCAGGAC TGTTCCGAGG CCGTGGGGAC CACCCAAAGA TGGGAATGCT GAAGCTGAGG 
    ATAATGCCAG AGGATGTGAT TATAAACTGC AGCAAAGAAT CCAAGGTTCC AGACCCCCCT 
    CCGGGCCACA AGTGGAAAGA GGTTCGGTGC GACAACACCG TGACATGGCT GGCATCATGG 
    ACGGAAAATA TCCAGAGTTC TATTAAGTAC ATCATGCTGA ACCCAAGCTC CAAACTGAAG 
    GGGGAGAAAG ACTGGCAGAA GTACGAGACT GCTCGCCGAC TGAAAGGGGT TGTGAGCGAC 
    ATCAGAGCTC AGTACCGAGT AGACTGGAAA TCCCGGGAGA TGAAGAAACG GCAGAGAGCT 
    GTGGCCCTCT ATTTCATTGA TAAGCTGGCA CTCCGAGCGG GAAATGAAAA AGAGGAAGGG 
    GAGACGGCAG ATACAGTTGG CTGCTGTTCC CTTAGGGTGG AGCACATACA GCTGTACCCA 
    GAGCTCGATG GGCAGCAGTA CGTGGTGGAG TTTGACTTCC TGGGAAAGGA TTCCATCCGC 
    TATTATAACA AGGTGCCAGT GGAGAAGGAG GTGTTTAAGA ACCTGAAGCT TTTCATGGAG 
    AATAAAAACC CGGAGGATGA TCTTTTCGAC AGGCTGAATA CTTCCATACT GAACAAACAT 
    CTACAGGATC TGATGGATGG ACTTACGGCC AAAGTGTTCC GAACCTACAA TGCTTCTATC 
    ACCCTGCAGA ACCAGCTGAA GGAGCTGACC AATGGGGAGG ACAATGTAGC GGCCAAGATT 
    CTGTCTTATA ATCGGGCAAA CAGAGCTGTA GCTCTTCTCT GTAACCACCA GAGGGCGCCA 
    CCAAAAACCT TTGAAAAGTC AATGCAGAAT CTACAGACTA AGATTGATGC AAAGGAACAG 
    CAGCTGAAAG AAGCAAAGAA AGAGTTAAAG AGCGCAAAGG CAGAGCATAA AGCCAGGAAG 
    AGTGAGAAGT CTAAGAAGGA GATGGAGAAG AAGAAGAAGC AAGTGGCACG GATAGAGGAG 
    CAGCTGATGA AGCTGAAGTT ACAGGCGACC GACAAAGAGG AGAATAAGCA GATTGCCCTG 
    GGCACGTCCA AACTCAACTA TCTGGATCCC AGGATCACCG TAGCCTGTTC TCTGTTTGGT 
    CTGCCCCAGG TGCAAGAAGT ACGGCGTGCC CATCGAGAAG ATCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    top1mt ( XM_004919865.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004919922.1 Xenopus tropicalis topoisomerase (DNA) I, mitochondrial (top1mt), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa19269
    Clone ID Related Accession (Same CDS sequence) XM_031903848.1 , XM_004919865.3
    Accession Version XM_004919865.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase I, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004919865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGGTATTT 
    CCTTCTCTTA CCATTGGTCT GTCTGCAGTC ACGTTTATAC ATCAAGCGCT TATGTACCCA 
    GAGCCGGAGA GGGGCCACAA GGAGAAGGGC CACAGTAAAT CTGAACCTGT GAAGCTGAAC 
    AGAAGCAGTC TAGTCAGGGA TGAGACCGTA TCTTCTACCA ACCCCACTGC TGAGGAGCCT 
    CCCAGGAGGA GGAAGCACAG ACTGGAGAAG GATGAAAAGG CCAAGTCTGC CGAAAGTTCC 
    GCTCATATAA AGAAAATCAA ATCTCACAAT CACCACACAG AAAGTGATAC TGGAGAGCCC 
    CTACCCAAGC ACAAAAAGGC AGACAATTCC CTCACAGAAA AGCTGCTGCA GGACGGTGTG 
    ATCTCCAGCG CCGCAGAAAA TGGGTTCTAT AAGTCAGTGG AACCGAGTGA TGGCAGAAAG 
    GAGATGTCTT GTGAAGTCAC CACCCGACTG GATGAGAACA GCAACATCAC CGCAATGGAG 
    TGCAATGGGG AATTTACACT TTACAGAGAG GATAAGGGGG TAGAATCTCC GTGCCAAACC 
    AGGAAAAATG AACCTAACTG GGAGAGTTCT ACTGCTGAAC CCAATGCTGG AGAAGAGACG 
    AGAAGAGAAG GACTCTGGGA GAGTACGGCA GATAGTAGGG CTACACTGGA CCCCCGCATA 
    AGCAAAGATA ACAGAAATAT ACAGGAACAA AAAGGGGGGT TGCCATGCAA AACTGAAAAG 
    GAAAGAGACG GCCATCACAA ATCCAAAGTA AAAAAGGATA ACGTTGATAG GTCTGAGAGG 
    AAAAAGGATC ATGTGGAGAA AAGTGGGGAT TCAGAGAAAA AGGAAAAGGG GGATTCAGAG 
    AAAAAGGAAA GGGGGAAAAA CCATTTGTGT AGAGATTCAG AGGGGGGGAA AGAAAGTTTA 
    AAAAGGGGCA AAGAAAACAA AGAGTCTAAA AGCGAGCAGA AAGCCATTGA TGGGACTGAA 
    GGCATCGCCC AGCCAGAAAG AGAGAAGAAA TGTGAAGATA AAATGGCATC AGGGAAAGAG 
    AGAGAATCTG CCGAGGAGAA GGAAACTGGT AAGAGAGACC CCCTGAAATC CAAAGATAAA 
    ACATCTGCTG GGGAGGAAAG AAAGCCAAAA AAGCATTCTC ACAAAGAGAA GTCCAAAGAG 
    ATGAACAGAA CAAGAGAGAA GATGGAAGAT GCTAAGATGT CAGTGTCTCG TGACCATCAC 
    AGGAAAGGAG ATGCCCTGGA GGGGGCAGTG GAAGCCAACG AGGCAGTAAA ACCTGAAAGA 
    ATTGAGGTGG CTGTTGGGGA ACCCAAGGAA CACAGCAGGG AAGTAGATGA GAAGGTCCAT 
    CTCAGGGTAA AGAGCTTCGA GGAGGAGCTG AATGAGTTGG CCAACCTGAA AATGCAGAGC 
    AGCAAAGTGA AGAAGAAGGA GAGGGAAACG AAGAAGCACG AGGAGAAGAG AGAGAAGATA 
    AAGGAGCAAA GCAGCGATGG GGCTGAAGGT AAAGCTACCA AAAGGGAAGG TGAGGAAGAG 
    GAGTTGGTGG GTGAGGAGGC CAAACCTAGG GTAGAAAAGA ATCAGAAAGA GAAGATTAAA 
    GAGAGAGGAA AAAAAAGCTC CAAGGATGAG GCCGCTAGAG ACAAAAAGCA GCCTTCTCGG 
    AAACGCAAAA GCACTGAAAC CGAAGGGACG GAAGCCAAAA AGGCAAAAGA TGCGGCCAAA 
    AAGGAGGAAG AATCAAAGTG GAAATGGTGG GAGGAGGAGC ACTACGAGGA TGGCATGAAG 
    TGGAAATTCC TGGAACACAG GGGCCCATAT TTTGCCCCTC CGTATGAGCC CCTCCCGGAT 
    GATGTCAGCT TCTACTATGA TGGGAAGCCA GTGAAACTGA GCCCAGGGGC CGAGGAAGTG 
    GCCACTTTCT ATGGCAAAAT GCTGGACCAT GAGTATACTA CCAAGGAGGC GTTCCAGAAG 
    AACTTCTTTG CAGACTGGAG AGAGCAAATG ACGAATGAGG AGCGGAAGCT GATCAAGCAC 
    CTACACAAGT GTGAATTCTC CGAGATCCAC AAGTATTTCT TTGAAAAGAA TGAGGCCCGC 
    AAGGCTTTGC CCAAGGAAGA GAAACAGAAA CTGAAGGAGG AAACCAATAA GATCCAGGAG 
    GAGTACGGCT ACTGCATCCT TGATGGACAC AGGGAAAAAA TTGGAAACTT CAAGATCGAG 
    CCCCCAGGAC TGTTCCGAGG CCGTGGGGAC CACCCAAAGA TGGGAATGCT GAAGCTGAGG 
    ATAATGCCAG AGGATGTGAT TATAAACTGC AGCAAAGAAT CCAAGGTTCC AGACCCCCCT 
    CCGGGCCACA AGTGGAAAGA GGTTCGGTGC GACAACACCG TGACATGGCT GGCATCATGG 
    ACGGAAAATA TCCAGAGTTC TATTAAGTAC ATCATGCTGA ACCCAAGCTC CAAACTGAAG 
    GGGGAGAAAG ACTGGCAGAA GTACGAGACT GCTCGCCGAC TGAAAGGGGT TGTGAGCGAC 
    ATCAGAGCTC AGTACCGAGT AGACTGGAAA TCCCGGGAGA TGAAGAAACG GCAGAGAGCT 
    GTGGCCCTCT ATTTCATTGA TAAGCTGGCA CTCCGAGCGG GAAATGAAAA AGAGGAAGGG 
    GAGACGGCAG ATACAGTTGG CTGCTGTTCC CTTAGGGTGG AGCACATACA GCTGTACCCA 
    GAGCTCGATG GGCAGCAGTA CGTGGTGGAG TTTGACTTCC TGGGAAAGGA TTCCATCCGC 
    TATTATAACA AGGTGCCAGT GGAGAAGGAG GTGTTTAAGA ACCTGAAGCT TTTCATGGAG 
    AATAAAAACC CGGAGGATGA TCTTTTCGAC AGGCTGAATA CTTCCATACT GAACAAACAT 
    CTACAGGATC TGATGGATGG ACTTACGGCC AAAGTGTTCC GAACCTACAA TGCTTCTATC 
    ACCCTGCAGA ACCAGCTGAA GGAGCTGACC AATGGGGAGG ACAATGTAGC GGCCAAGATT 
    CTGTCTTATA ATCGGGCAAA CAGAGCTGTA GCTCTTCTCT GTAACCACCA GAGGGCGCCA 
    CCAAAAACCT TTGAAAAGTC AATGCAGAAT CTACAGACTA AGATTGATGC AAAGGAACAG 
    CAGCTGAAAG AAGCAAAGAA AGAGTTAAAG AGCGCAAAGG CAGAGCATAA AGCCAGGAAG 
    AGTGAGAAGT CTAAGAAGGA GATGGAGAAG AAGAAGAAGC AAGTGGCACG GATAGAGGAG 
    CAGCTGATGA AGCTGAAGTT ACAGGCGACC GACAAAGAGG AGAATAAGCA GATTGCCCTG 
    GGCACGTCCA AACTCAACTA TCTGGATCCC AGGATCACCG TAGCCTGGTG CAAGAAGTAC 
    GGCGTGCCCA TCGAGAAGAT CTATAACAAG ACGCAGAGAG AGAAATTTGC GTGGGCGATA 
    GACATGACCA GCGAGAACTT TGAGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004919922.1
    CDS67..3516
    Translation

    Target ORF information:

    RefSeq Version XM_004919865.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) I, mitochondrial (top1mt), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004919865.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGGTATTT 
    CCTTCTCTTA CCATTGGTCT GTCTGCAGTC ACGTTTATAC ATCAAGCGCT TATGTACCCA 
    GAGCCGGAGA GGGGCCACAA GGAGAAGGGC CACAGTAAAT CTGAACCTGT GAAGCTGAAC 
    AGAAGCAGTC TAGTCAGGGA TGAGACCGTA TCTTCTACCA ACCCCACTGC TGAGGAGCCT 
    CCCAGGAGGA GGAAGCACAG ACTGGAGAAG GATGAAAAGG CCAAGTCTGC CGAAAGTTCC 
    GCTCATATAA AGAAAATCAA ATCTCACAAT CACCACACAG AAAGTGATAC TGGAGAGCCC 
    CTACCCAAGC ACAAAAAGGC AGACAATTCC CTCACAGAAA AGCTGCTGCA GGACGGTGTG 
    ATCTCCAGCG CCGCAGAAAA TGGGTTCTAT AAGTCAGTGG AACCGAGTGA TGGCAGAAAG 
    GAGATGTCTT GTGAAGTCAC CACCCGACTG GATGAGAACA GCAACATCAC CGCAATGGAG 
    TGCAATGGGG AATTTACACT TTACAGAGAG GATAAGGGGG TAGAATCTCC GTGCCAAACC 
    AGGAAAAATG AACCTAACTG GGAGAGTTCT ACTGCTGAAC CCAATGCTGG AGAAGAGACG 
    AGAAGAGAAG GACTCTGGGA GAGTACGGCA GATAGTAGGG CTACACTGGA CCCCCGCATA 
    AGCAAAGATA ACAGAAATAT ACAGGAACAA AAAGGGGGGT TGCCATGCAA AACTGAAAAG 
    GAAAGAGACG GCCATCACAA ATCCAAAGTA AAAAAGGATA ACGTTGATAG GTCTGAGAGG 
    AAAAAGGATC ATGTGGAGAA AAGTGGGGAT TCAGAGAAAA AGGAAAAGGG GGATTCAGAG 
    AAAAAGGAAA GGGGGAAAAA CCATTTGTGT AGAGATTCAG AGGGGGGGAA AGAAAGTTTA 
    AAAAGGGGCA AAGAAAACAA AGAGTCTAAA AGCGAGCAGA AAGCCATTGA TGGGACTGAA 
    GGCATCGCCC AGCCAGAAAG AGAGAAGAAA TGTGAAGATA AAATGGCATC AGGGAAAGAG 
    AGAGAATCTG CCGAGGAGAA GGAAACTGGT AAGAGAGACC CCCTGAAATC CAAAGATAAA 
    ACATCTGCTG GGGAGGAAAG AAAGCCAAAA AAGCATTCTC ACAAAGAGAA GTCCAAAGAG 
    ATGAACAGAA CAAGAGAGAA GATGGAAGAT GCTAAGATGT CAGTGTCTCG TGACCATCAC 
    AGGAAAGGAG ATGCCCTGGA GGGGGCAGTG GAAGCCAACG AGGCAGTAAA ACCTGAAAGA 
    ATTGAGGTGG CTGTTGGGGA ACCCAAGGAA CACAGCAGGG AAGTAGATGA GAAGGTCCAT 
    CTCAGGGTAA AGAGCTTCGA GGAGGAGCTG AATGAGTTGG CCAACCTGAA AATGCAGAGC 
    AGCAAAGTGA AGAAGAAGGA GAGGGAAACG AAGAAGCACG AGGAGAAGAG AGAGAAGATA 
    AAGGAGCAAA GCAGCGATGG GGCTGAAGGT AAAGCTACCA AAAGGGAAGG TGAGGAAGAG 
    GAGTTGGTGG GTGAGGAGGC CAAACCTAGG GTAGAAAAGA ATCAGAAAGA GAAGATTAAA 
    GAGAGAGGAA AAAAAAGCTC CAAGGATGAG GCCGCTAGAG ACAAAAAGCA GCCTTCTCGG 
    AAACGCAAAA GCACTGAAAC CGAAGGGACG GAAGCCAAAA AGGCAAAAGA TGCGGCCAAA 
    AAGGAGGAAG AATCAAAGTG GAAATGGTGG GAGGAGGAGC ACTACGAGGA TGGCATGAAG 
    TGGAAATTCC TGGAACACAG GGGCCCATAT TTTGCCCCTC CGTATGAGCC CCTCCCGGAT 
    GATGTCAGCT TCTACTATGA TGGGAAGCCA GTGAAACTGA GCCCAGGGGC CGAGGAAGTG 
    GCCACTTTCT ATGGCAAAAT GCTGGACCAT GAGTATACTA CCAAGGAGGC GTTCCAGAAG 
    AACTTCTTTG CAGACTGGAG AGAGCAAATG ACGAATGAGG AGCGGAAGCT GATCAAGCAC 
    CTACACAAGT GTGAATTCTC CGAGATCCAC AAGTATTTCT TTGAAAAGAA TGAGGCCCGC 
    AAGGCTTTGC CCAAGGAAGA GAAACAGAAA CTGAAGGAGG AAACCAATAA GATCCAGGAG 
    GAGTACGGCT ACTGCATCCT TGATGGACAC AGGGAAAAAA TTGGAAACTT CAAGATCGAG 
    CCCCCAGGAC TGTTCCGAGG CCGTGGGGAC CACCCAAAGA TGGGAATGCT GAAGCTGAGG 
    ATAATGCCAG AGGATGTGAT TATAAACTGC AGCAAAGAAT CCAAGGTTCC AGACCCCCCT 
    CCGGGCCACA AGTGGAAAGA GGTTCGGTGC GACAACACCG TGACATGGCT GGCATCATGG 
    ACGGAAAATA TCCAGAGTTC TATTAAGTAC ATCATGCTGA ACCCAAGCTC CAAACTGAAG 
    GGGGAGAAAG ACTGGCAGAA GTACGAGACT GCTCGCCGAC TGAAAGGGGT TGTGAGCGAC 
    ATCAGAGCTC AGTACCGAGT AGACTGGAAA TCCCGGGAGA TGAAGAAACG GCAGAGAGCT 
    GTGGCCCTCT ATTTCATTGA TAAGCTGGCA CTCCGAGCGG GAAATGAAAA AGAGGAAGGG 
    GAGACGGCAG ATACAGTTGG CTGCTGTTCC CTTAGGGTGG AGCACATACA GCTGTACCCA 
    GAGCTCGATG GGCAGCAGTA CGTGGTGGAG TTTGACTTCC TGGGAAAGGA TTCCATCCGC 
    TATTATAACA AGGTGCCAGT GGAGAAGGAG GTGTTTAAGA ACCTGAAGCT TTTCATGGAG 
    AATAAAAACC CGGAGGATGA TCTTTTCGAC AGGCTGAATA CTTCCATACT GAACAAACAT 
    CTACAGGATC TGATGGATGG ACTTACGGCC AAAGTGTTCC GAACCTACAA TGCTTCTATC 
    ACCCTGCAGA ACCAGCTGAA GGAGCTGACC AATGGGGAGG ACAATGTAGC GGCCAAGATT 
    CTGTCTTATA ATCGGGCAAA CAGAGCTGTA GCTCTTCTCT GTAACCACCA GAGGGCGCCA 
    CCAAAAACCT TTGAAAAGTC AATGCAGAAT CTACAGACTA AGATTGATGC AAAGGAACAG 
    CAGCTGAAAG AAGCAAAGAA AGAGTTAAAG AGCGCAAAGG CAGAGCATAA AGCCAGGAAG 
    AGTGAGAAGT CTAAGAAGGA GATGGAGAAG AAGAAGAAGC AAGTGGCACG GATAGAGGAG 
    CAGCTGATGA AGCTGAAGTT ACAGGCGACC GACAAAGAGG AGAATAAGCA GATTGCCCTG 
    GGCACGTCCA AACTCAACTA TCTGGATCCC AGGATCACCG TAGCCTGGTG CAAGAAGTAC 
    GGCGTGCCCA TCGAGAAGAT CTATAACAAG ACGCAGAGAG AGAAATTTGC GTGGGCGATA 
    GACATGACCA GCGAGAACTT TGAGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    top1mt ( XM_004919866.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004919923.1 Xenopus tropicalis topoisomerase (DNA) I, mitochondrial (top1mt), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OXa19270
    Clone ID Related Accession (Same CDS sequence) XM_004919866.3 , XM_031903850.1 , XM_031903851.1 , XM_031903852.1
    Accession Version XM_004919866.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase I, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004919866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGAGGGGC 
    CACAAGGAGA AGGGCCACAG TAAATCTGAA CCTGTGAAGC TGAACAGAAG CAGTCTAGTC 
    AGGGATGAGA CCGTATCTTC TACCAACCCC ACTGCTGAGG AGCCTCCCAG GAGGAGGAAG 
    CACAGACTGG AGAAGGATGA AAAGGCCAAG TCTGCCGAAA GTTCCGCTCA TATAAAGAAA 
    ATCAAATCTC ACAATCACCA CACAGAAAGT GATACTGGAG AGCCCCTACC CAAGCACAAA 
    AAGGCAGACA ATTCCCTCAC AGAAAAGCTG CTGCAGGACG GTGTGATCTC CAGCGCCGCA 
    GAAAATGGGT TCTATAAGTC AGTGGAACCG AGTGATGGCA GAAAGGAGAT GTCTTGTGAA 
    GTCACCACCC GACTGGATGA GAACAGCAAC ATCACCGCAA TGGAGTGCAA TGGGGAATTT 
    ACACTTTACA GAGAGGATAA GGGGGTAGAA TCTCCGTGCC AAACCAGGAA AAATGAACCT 
    AACTGGGAGA GTTCTACTGC TGAACCCAAT GCTGGAGAAG AGACGAGAAG AGAAGGACTC 
    TGGGAGAGTA CGGCAGATAG TAGGGCTACA CTGGACCCCC GCATAAGCAA AGATAACAGA 
    AATATACAGG AACAAAAAGG GGGGTTGCCA TGCAAAACTG AAAAGGAAAG AGACGGCCAT 
    CACAAATCCA AAGTAAAAAA GGATAACGTT GATAGGTCTG AGAGGAAAAA GGATCATGTG 
    GAGAAAAGTG GGGATTCAGA GAAAAAGGAA AAGGGGGATT CAGAGAAAAA GGAAAGGGGG 
    AAAAACCATT TGTGTAGAGA TTCAGAGGGG GGGAAAGAAA GTTTAAAAAG GGGCAAAGAA 
    AACAAAGAGT CTAAAAGCGA GCAGAAAGCC ATTGATGGGA CTGAAGGCAT CGCCCAGCCA 
    GAAAGAGAGA AGAAATGTGA AGATAAAATG GCATCAGGGA AAGAGAGAGA ATCTGCCGAG 
    GAGAAGGAAA CTGGTAAGAG AGACCCCCTG AAATCCAAAG ATAAAACATC TGCTGGGGAG 
    GAAAGAAAGC CAAAAAAGCA TTCTCACAAA GAGAAGTCCA AAGAGATGAA CAGAACAAGA 
    GAGAAGATGG AAGATGCTAA GATGTCAGTG TCTCGTGACC ATCACAGGAA AGGAGATGCC 
    CTGGAGGGGG CAGTGGAAGC CAACGAGGCA GTAAAACCTG AAAGAATTGA GGTGGCTGTT 
    GGGGAACCCA AGGAACACAG CAGGGAAGTA GATGAGAAGG TCCATCTCAG GGTAAAGAGC 
    TTCGAGGAGG AGCTGAATGA GTTGGCCAAC CTGAAAATGC AGAGCAGCAA AGTGAAGAAG 
    AAGGAGAGGG AAACGAAGAA GCACGAGGAG AAGAGAGAGA AGATAAAGGA GCAAAGCAGC 
    GATGGGGCTG AAGGTAAAGC TACCAAAAGG GAAGGTGAGG AAGAGGAGTT GGTGGGTGAG 
    GAGGCCAAAC CTAGGGTAGA AAAGAATCAG AAAGAGAAGA TTAAAGAGAG AGGAAAAAAA 
    AGCTCCAAGG ATGAGGCCGC TAGAGACAAA AAGCAGCCTT CTCGGAAACG CAAAAGCACT 
    GAAACCGAAG GGACGGAAGC CAAAAAGGCA AAAGATGCGG CCAAAAAGGA GGAAGAATCA 
    AAGTGGAAAT GGTGGGAGGA GGAGCACTAC GAGGATGGCA TGAAGTGGAA ATTCCTGGAA 
    CACAGGGGCC CATATTTTGC CCCTCCGTAT GAGCCCCTCC CGGATGATGT CAGCTTCTAC 
    TATGATGGGA AGCCAGTGAA ACTGAGCCCA GGGGCCGAGG AAGTGGCCAC TTTCTATGGC 
    AAAATGCTGG ACCATGAGTA TACTACCAAG GAGGCGTTCC AGAAGAACTT CTTTGCAGAC 
    TGGAGAGAGC AAATGACGAA TGAGGAGCGG AAGCTGATCA AGCACCTACA CAAGTGTGAA 
    TTCTCCGAGA TCCACAAGTA TTTCTTTGAA AAGAATGAGG CCCGCAAGGC TTTGCCCAAG 
    GAAGAGAAAC AGAAACTGAA GGAGGAAACC AATAAGATCC AGGAGGAGTA CGGCTACTGC 
    ATCCTTGATG GACACAGGGA AAAAATTGGA AACTTCAAGA TCGAGCCCCC AGGACTGTTC 
    CGAGGCCGTG GGGACCACCC AAAGATGGGA ATGCTGAAGC TGAGGATAAT GCCAGAGGAT 
    GTGATTATAA ACTGCAGCAA AGAATCCAAG GTTCCAGACC CCCCTCCGGG CCACAAGTGG 
    AAAGAGGTTC GGTGCGACAA CACCGTGACA TGGCTGGCAT CATGGACGGA AAATATCCAG 
    AGTTCTATTA AGTACATCAT GCTGAACCCA AGCTCCAAAC TGAAGGGGGA GAAAGACTGG 
    CAGAAGTACG AGACTGCTCG CCGACTGAAA GGGGTTGTGA GCGACATCAG AGCTCAGTAC 
    CGAGTAGACT GGAAATCCCG GGAGATGAAG AAACGGCAGA GAGCTGTGGC CCTCTATTTC 
    ATTGATAAGC TGGCACTCCG AGCGGGAAAT GAAAAAGAGG AAGGGGAGAC GGCAGATACA 
    GTTGGCTGCT GTTCCCTTAG GGTGGAGCAC ATACAGCTGT ACCCAGAGCT CGATGGGCAG 
    CAGTACGTGG TGGAGTTTGA CTTCCTGGGA AAGGATTCCA TCCGCTATTA TAACAAGGTG 
    CCAGTGGAGA AGGAGGTGTT TAAGAACCTG AAGCTTTTCA TGGAGAATAA AAACCCGGAG 
    GATGATCTTT TCGACAGGCT GAATACTTCC ATACTGAACA AACATCTACA GGATCTGATG 
    GATGGACTTA CGGCCAAAGT GTTCCGAACC TACAATGCTT CTATCACCCT GCAGAACCAG 
    CTGAAGGAGC TGACCAATGG GGAGGACAAT GTAGCGGCCA AGATTCTGTC TTATAATCGG 
    GCAAACAGAG CTGTAGCTCT TCTCTGTAAC CACCAGAGGG CGCCACCAAA AACCTTTGAA 
    AAGTCAATGC AGAATCTACA GACTAAGATT GATGCAAAGG AACAGCAGCT GAAAGAAGCA 
    AAGAAAGAGT TAAAGAGCGC AAAGGCAGAG CATAAAGCCA GGAAGAGTGA GAAGTCTAAG 
    AAGGAGATGG AGAAGAAGAA GAAGCAAGTG GCACGGATAG AGGAGCAGCT GATGAAGCTG 
    AAGTTACAGG CGACCGACAA AGAGGAGAAT AAGCAGATTG CCCTGGGCAC GTCCAAACTC 
    AACTATCTGG ATCCCAGGAT CACCGTAGCC TGGTGCAAGA AGTACGGCGT GCCCATCGAG 
    AAGATCTATA ACAAGACGCA GAGAGAGAAA TTTGCGTGGG CGATAGACAT GACCAGCGAG 
    AACTTTGAGT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004919923.1
    CDS65..3439
    Translation

    Target ORF information:

    RefSeq Version XM_004919866.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) I, mitochondrial (top1mt), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004919866.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAG ATGACGCTCA GACTCTCCAT CGCCGCGGGG AGGATGCAAA ATCGAGGGGC 
    CACAAGGAGA AGGGCCACAG TAAATCTGAA CCTGTGAAGC TGAACAGAAG CAGTCTAGTC 
    AGGGATGAGA CCGTATCTTC TACCAACCCC ACTGCTGAGG AGCCTCCCAG GAGGAGGAAG 
    CACAGACTGG AGAAGGATGA AAAGGCCAAG TCTGCCGAAA GTTCCGCTCA TATAAAGAAA 
    ATCAAATCTC ACAATCACCA CACAGAAAGT GATACTGGAG AGCCCCTACC CAAGCACAAA 
    AAGGCAGACA ATTCCCTCAC AGAAAAGCTG CTGCAGGACG GTGTGATCTC CAGCGCCGCA 
    GAAAATGGGT TCTATAAGTC AGTGGAACCG AGTGATGGCA GAAAGGAGAT GTCTTGTGAA 
    GTCACCACCC GACTGGATGA GAACAGCAAC ATCACCGCAA TGGAGTGCAA TGGGGAATTT 
    ACACTTTACA GAGAGGATAA GGGGGTAGAA TCTCCGTGCC AAACCAGGAA AAATGAACCT 
    AACTGGGAGA GTTCTACTGC TGAACCCAAT GCTGGAGAAG AGACGAGAAG AGAAGGACTC 
    TGGGAGAGTA CGGCAGATAG TAGGGCTACA CTGGACCCCC GCATAAGCAA AGATAACAGA 
    AATATACAGG AACAAAAAGG GGGGTTGCCA TGCAAAACTG AAAAGGAAAG AGACGGCCAT 
    CACAAATCCA AAGTAAAAAA GGATAACGTT GATAGGTCTG AGAGGAAAAA GGATCATGTG 
    GAGAAAAGTG GGGATTCAGA GAAAAAGGAA AAGGGGGATT CAGAGAAAAA GGAAAGGGGG 
    AAAAACCATT TGTGTAGAGA TTCAGAGGGG GGGAAAGAAA GTTTAAAAAG GGGCAAAGAA 
    AACAAAGAGT CTAAAAGCGA GCAGAAAGCC ATTGATGGGA CTGAAGGCAT CGCCCAGCCA 
    GAAAGAGAGA AGAAATGTGA AGATAAAATG GCATCAGGGA AAGAGAGAGA ATCTGCCGAG 
    GAGAAGGAAA CTGGTAAGAG AGACCCCCTG AAATCCAAAG ATAAAACATC TGCTGGGGAG 
    GAAAGAAAGC CAAAAAAGCA TTCTCACAAA GAGAAGTCCA AAGAGATGAA CAGAACAAGA 
    GAGAAGATGG AAGATGCTAA GATGTCAGTG TCTCGTGACC ATCACAGGAA AGGAGATGCC 
    CTGGAGGGGG CAGTGGAAGC CAACGAGGCA GTAAAACCTG AAAGAATTGA GGTGGCTGTT 
    GGGGAACCCA AGGAACACAG CAGGGAAGTA GATGAGAAGG TCCATCTCAG GGTAAAGAGC 
    TTCGAGGAGG AGCTGAATGA GTTGGCCAAC CTGAAAATGC AGAGCAGCAA AGTGAAGAAG 
    AAGGAGAGGG AAACGAAGAA GCACGAGGAG AAGAGAGAGA AGATAAAGGA GCAAAGCAGC 
    GATGGGGCTG AAGGTAAAGC TACCAAAAGG GAAGGTGAGG AAGAGGAGTT GGTGGGTGAG 
    GAGGCCAAAC CTAGGGTAGA AAAGAATCAG AAAGAGAAGA TTAAAGAGAG AGGAAAAAAA 
    AGCTCCAAGG ATGAGGCCGC TAGAGACAAA AAGCAGCCTT CTCGGAAACG CAAAAGCACT 
    GAAACCGAAG GGACGGAAGC CAAAAAGGCA AAAGATGCGG CCAAAAAGGA GGAAGAATCA 
    AAGTGGAAAT GGTGGGAGGA GGAGCACTAC GAGGATGGCA TGAAGTGGAA ATTCCTGGAA 
    CACAGGGGCC CATATTTTGC CCCTCCGTAT GAGCCCCTCC CGGATGATGT CAGCTTCTAC 
    TATGATGGGA AGCCAGTGAA ACTGAGCCCA GGGGCCGAGG AAGTGGCCAC TTTCTATGGC 
    AAAATGCTGG ACCATGAGTA TACTACCAAG GAGGCGTTCC AGAAGAACTT CTTTGCAGAC 
    TGGAGAGAGC AAATGACGAA TGAGGAGCGG AAGCTGATCA AGCACCTACA CAAGTGTGAA 
    TTCTCCGAGA TCCACAAGTA TTTCTTTGAA AAGAATGAGG CCCGCAAGGC TTTGCCCAAG 
    GAAGAGAAAC AGAAACTGAA GGAGGAAACC AATAAGATCC AGGAGGAGTA CGGCTACTGC 
    ATCCTTGATG GACACAGGGA AAAAATTGGA AACTTCAAGA TCGAGCCCCC AGGACTGTTC 
    CGAGGCCGTG GGGACCACCC AAAGATGGGA ATGCTGAAGC TGAGGATAAT GCCAGAGGAT 
    GTGATTATAA ACTGCAGCAA AGAATCCAAG GTTCCAGACC CCCCTCCGGG CCACAAGTGG 
    AAAGAGGTTC GGTGCGACAA CACCGTGACA TGGCTGGCAT CATGGACGGA AAATATCCAG 
    AGTTCTATTA AGTACATCAT GCTGAACCCA AGCTCCAAAC TGAAGGGGGA GAAAGACTGG 
    CAGAAGTACG AGACTGCTCG CCGACTGAAA GGGGTTGTGA GCGACATCAG AGCTCAGTAC 
    CGAGTAGACT GGAAATCCCG GGAGATGAAG AAACGGCAGA GAGCTGTGGC CCTCTATTTC 
    ATTGATAAGC TGGCACTCCG AGCGGGAAAT GAAAAAGAGG AAGGGGAGAC GGCAGATACA 
    GTTGGCTGCT GTTCCCTTAG GGTGGAGCAC ATACAGCTGT ACCCAGAGCT CGATGGGCAG 
    CAGTACGTGG TGGAGTTTGA CTTCCTGGGA AAGGATTCCA TCCGCTATTA TAACAAGGTG 
    CCAGTGGAGA AGGAGGTGTT TAAGAACCTG AAGCTTTTCA TGGAGAATAA AAACCCGGAG 
    GATGATCTTT TCGACAGGCT GAATACTTCC ATACTGAACA AACATCTACA GGATCTGATG 
    GATGGACTTA CGGCCAAAGT GTTCCGAACC TACAATGCTT CTATCACCCT GCAGAACCAG 
    CTGAAGGAGC TGACCAATGG GGAGGACAAT GTAGCGGCCA AGATTCTGTC TTATAATCGG 
    GCAAACAGAG CTGTAGCTCT TCTCTGTAAC CACCAGAGGG CGCCACCAAA AACCTTTGAA 
    AAGTCAATGC AGAATCTACA GACTAAGATT GATGCAAAGG AACAGCAGCT GAAAGAAGCA 
    AAGAAAGAGT TAAAGAGCGC AAAGGCAGAG CATAAAGCCA GGAAGAGTGA GAAGTCTAAG 
    AAGGAGATGG AGAAGAAGAA GAAGCAAGTG GCACGGATAG AGGAGCAGCT GATGAAGCTG 
    AAGTTACAGG CGACCGACAA AGAGGAGAAT AAGCAGATTG CCCTGGGCAC GTCCAAACTC 
    AACTATCTGG ATCCCAGGAT CACCGTAGCC TGGTGCAAGA AGTACGGCGT GCCCATCGAG 
    AAGATCTATA ACAAGACGCA GAGAGAGAAA TTTGCGTGGG CGATAGACAT GACCAGCGAG 
    AACTTTGAGT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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