Ndufv2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndufv2
Entrez Gene ID	72900
Full Name	NADH dehydrogenase (ubiquinone) flavoprotein 2
Synonyms	2900010C23Rik
General protein information	Preferred Names NADH dehydrogenase (ubiquinone) flavoprotein 2 Names NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial NADH-ubiquinone oxidoreductase 24 kDa subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 35.26 cM\|17 E1.1
Summary	This gene encodes a subunit of the NADH-ubiquinone oxidoreductase (complex I) enzyme, which is a large, multimeric protein. It is the first enzyme complex in the mitochondrial electron transport chain and catalyzes the transfer of electrons from NADH to the electron acceptor ubiquinone. The proton gradient created by electron transfer drives the conversion of ADP to ATP. This gene is a core subunit and is conserved in prokaryotes and eukaryotes. The bovine ortholog of this protein has been characterized and is reported to contain an iron-sulfur cluster, which may be involved in electron transfer. In humans mutations in this gene are implicated in Parkinson's disease, bipolar disorder, schizophrenia, and have been found in one case of early onset hypertrophic cardiomyopathy and encephalopathy. A pseudogene of this gene is located on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_028388.3	NP_082664.1	NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial isoform 1 precursor
NM_001278415.1	NP_001265344.1	NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NDUFV2	NP_776990.1
Gallus gallus (chicken)	NDUFV2	XP_424129.3
Caenorhabditis elegans (roundworm)	CELE_F53F4.10	NP_506376.1
Magnaporthe oryzae (rice blast fungus)	MGG_07301	XP_003715530.1
Arabidopsis thaliana (thale cress)	AT4G02580	NP_567244.1
	NDUFV2	XP_001099724.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002170	XP_308018.4
Neurospora crassa	NCU01169	XP_961535.1
Xenopus tropicalis (tropical clawed frog)	ndufv2	NP_001017166.1
Pan troglodytes (chimpanzee)	NDUFV2	NP_001065254.1
	LOC713739	XP_001108313.1
Drosophila melanogaster (fruit fly)	CG5703	NP_573228.1
Oryza sativa (rice)	Os05g0509200	NP_001056000.1
Homo sapiens (human)	NDUFV2	NP_066552.2
Canis lupus familiaris (dog)	NDUFV2	XP_537328.2
Mus musculus (house mouse)	Ndufv2	NP_082664.1
Rattus norvegicus (Norway rat)	Ndufv2	NP_112326.1
Danio rerio (zebrafish)	ndufv2	NP_957041.1

Gene Ontology
Bibliography

Related articles in PubMed

NDUFV2 regulates neuronal migration in the developing cerebral cortex through modulation of the multipolar-bipolar transition.
Chen T, Wu Q, Zhang Y, Zhang D
Brain research1625102-10(2015 Nov)

Subunit-specific incorporation efficiency and kinetics in mitochondrial complex I homeostasis.
Dieteren CE, Koopman WJ, Swarts HG, Peters JG, Maczuga P, van Gemst JJ, Masereeuw R, Smeitink JA, Nijtmans LG, Willems PH
The Journal of biological chemistry287(50)41851-60(2012 Dec)

Molecular base of biochemical complex I deficiency.
Hoefs SJ, Rodenburg RJ, Smeitink JA, van den Heuvel LP
Mitochondrion12(5)520-32(2012 Sep)

Region-specific expression of mitochondrial complex I genes during murine brain development.
Wirtz S, Schuelke M
PloS one6(4)e18897(2011 Apr)

Proteolipid protein is required for transport of sirtuin 2 into CNS myelin.
Werner HB, Kuhlmann K, Shen S, Uecker M, Schardt A, Dimova K, Orfaniotou F, Dhaunchak A, Brinkmann BG, M?bius W, Guarente L, Casaccia-Bonnefil P, Jahn O, Nave KA
The Journal of neuroscience : the official journal of the Society for Neuroscience27(29)7717-30(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of this study indicated that ndufv2 knockdown influences the actin cytoskeleton and tubulin stabilization in the leading process of migrating cortical neurons
Title: NDUFV2 regulates neuronal migration in the developing cerebral cortex through modulation of the multipolar-bipolar transition.

The authors use green fluorescent-tagged complex I subunits to determine the kinetics of assembly. Tagged human NDUFV2 can replace the endogenous protein in the holo-complex I protein and was also found in complex I subassemblies, suggesting that NDUFV2 may directly incorporate into the holo-enzyme and may also assemble into smaller complexes or in a monomer pool.
Title: Subunit-specific incorporation efficiency and kinetics in mitochondrial complex I homeostasis.

The authors provide an overview of the function and assembly of human complex I, also known as NADH dehydrogenase (ubiquinone) 1 alpha subcomplex. They discuss its role in oxidative phosphorylation and the implications of complex I deficiency in disease.
Title: Molecular base of biochemical complex I deficiency.

The authors biochemically separate the NADH dehydrogenase module from complex I of bovine heart into subunit I, II and III fractions. Subunit II fraction, also known as the NADH dehydrogenase (ubiquinone) flavoprotein 2 protein, has a molecular weight of 24 000 Da and contains an iron-sulfur center.
Title: Resolution of mitochondrial NADH dehydrogenase and isolation of two iron-sulfur proteins.

The following Ndufv2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndufv2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13700	NM_028388.3 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase core subunit V2 (Ndufv2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu16955	NM_001278415.1 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase core subunit V2 (Ndufv2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndufv2 ( NM_028388.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082664.1 Mus musculus NADH:ubiquinone oxidoreductase core subunit V2 (Ndufv2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13700
Clone ID Related Accession (Same CDS sequence)	NM_028388.3
Accession Version	NM_028388.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY246389.1 and AK146998.1. On Jun 5, 2013 this sequence version replaced NM_028388.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK146998.1, SRR1660811.308943.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 23038253; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCTCCT TGGCGCTGCG GGCCAGGGCG ACCGGCCTCG CTGCTCAGTG GGGAAGACAT 
GCAAGGAATT TGCATAAGAC AGCAGTGCAC AATGGTGCTG GAGGAGCCTT ATTTGTGCAT 
AGAGATACTC CTGAGAATAA CCCAGATACT CCATTTGATT TCACACCAGA AAACTATAAG 
AGGATAGAGG CAATAGTAAA AAACTACCCA GAAGGGCATC AAGCCGCTGC TGTGCTTCCA 
GTCCTGGATC TCGCCCAAAG GCAGAATGGA TGGCTACCTA TCTCCGCTAT GAACAAGGTG 
GCTGAAGTTT TACAAGTACC TCCAATGAGA GTATATGAAG TAGCAACTTT TTATACAATG 
TATAATCGAA AGCCAGTTGG GAAGTACCAT ATCCAGGTCT GCACTACTAC ACCTTGCATG 
CTGCGAGATT CTGACAGCAT ATTGGAGACC CTTCAGAGAA AGCTTGGAAT AAAGGTTGGA 
GAGACTACAC CTGACAAACT TTTCACTCTT ATAGAAGTGG AATGTTTAGG GGCCTGTGTA 
AATGCACCGA TGGTTCAAAT AAATGACAAC TACTATGAGG ATCTGACACC CAAGGATATT 
GAAGAGATTA TTGATGAACT CAAAGCTGGA AAAGTTCCCA AACCAGGGCC AAGGAGTGGC 
CGCTTCTGTT GTGAGCCAGC TGGAGGCCTT ACTTCTTTGA CTGAACCACC CAAAGGACCT 
GGCTTTGGTG TGCAAGCAGG CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082664.1
CDS	95..841
Translation	MFSLALRARATGLAAQWGRHARNLHKTAVHNGAGGALFVHRDTPENNPDTPFDFTPENYKRIEAIVKNYPEGHQAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDNYYEDLTPKDIEEIIDELKAGKVPKPGPRSGRFCCEPAGGLTSLTEPPKGPGFGVQAGL

Target ORF information:

RefSeq Version	NM_028388.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase core subunit V2 (Ndufv2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028388.3

ORF Insert Sequence:

ATGTTCTCCT TGGCGCTGCG GGCCAGGGCG ACCGGCCTCG CTGCTCAGTG GGGAAGACAT 
GCAAGGAATT TGCATAAGAC AGCAGTGCAC AATGGTGCTG GAGGAGCCTT ATTTGTGCAT 
AGAGATACTC CTGAGAATAA CCCAGATACT CCATTTGATT TCACACCAGA AAACTATAAG 
AGGATAGAGG CAATAGTAAA AAACTACCCA GAAGGGCATC AAGCCGCTGC TGTGCTTCCA 
GTCCTGGATC TCGCCCAAAG GCAGAATGGA TGGCTACCTA TCTCCGCTAT GAACAAGGTG 
GCTGAAGTTT TACAAGTACC TCCAATGAGA GTATATGAAG TAGCAACTTT TTATACAATG 
TATAATCGAA AGCCAGTTGG GAAGTACCAT ATCCAGGTCT GCACTACTAC ACCTTGCATG 
CTGCGAGATT CTGACAGCAT ATTGGAGACC CTTCAGAGAA AGCTTGGAAT AAAGGTTGGA 
GAGACTACAC CTGACAAACT TTTCACTCTT ATAGAAGTGG AATGTTTAGG GGCCTGTGTA 
AATGCACCGA TGGTTCAAAT AAATGACAAC TACTATGAGG ATCTGACACC CAAGGATATT 
GAAGAGATTA TTGATGAACT CAAAGCTGGA AAAGTTCCCA AACCAGGGCC AAGGAGTGGC 
CGCTTCTGTT GTGAGCCAGC TGGAGGCCTT ACTTCTTTGA CTGAACCACC CAAAGGACCT 
GGCTTTGGTG TGCAAGCAGG CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndufv2 ( NM_001278415.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001265344.1 Mus musculus NADH:ubiquinone oxidoreductase core subunit V2 (Ndufv2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16955
Clone ID Related Accession (Same CDS sequence)	NM_001278415.1
Accession Version	NM_001278415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY246389.1, AK150404.1, CF106105.1 and AK146998.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK150404.1, AK151729.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 23038253; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACAAGG TGGCTGAAGT TTTACAAGTA CCTCCAATGA GAGTATATGA AGTAGCAACT 
TTTTATACAA TGTATAATCG AAAGCCAGTT GGGAAGTACC ATATCCAGGT CTGCACTACT 
ACACCTTGCA TGCTGCGAGA TTCTGACAGC ATATTGGAGA CCCTTCAGAG AAAGCTTGGA 
ATAAAGGTTG GAGAGACTAC ACCTGACAAA CTTTTCACTC TTATAGAAGT GGAATGTTTA 
GGGGCCTGTG TAAATGCACC GATGGTTCAA ATAAATGACA ACTACTATGA GGATCTGACA 
CCCAAGGATA TTGAAGAGAT TATTGATGAA CTCAAAGCTG GAAAAGTTCC CAAACCAGGG 
CCAAGGAGTG GCCGCTTCTG TTGTGAGCCA GCTGGAGGCC TTACTTCTTT GACTGAACCA 
CCCAAAGGAC CTGGCTTTGG TGTGCAAGCA GGCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265344.1
CDS	480..938
Translation	MNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCMLRDSDSILETLQRKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDNYYEDLTPKDIEEIIDELKAGKVPKPGPRSGRFCCEPAGGLTSLTEPPKGPGFGVQAGL

Target ORF information:

RefSeq Version	NM_001278415.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase core subunit V2 (Ndufv2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278415.1

ORF Insert Sequence:

ATGAACAAGG TGGCTGAAGT TTTACAAGTA CCTCCAATGA GAGTATATGA AGTAGCAACT 
TTTTATACAA TGTATAATCG AAAGCCAGTT GGGAAGTACC ATATCCAGGT CTGCACTACT 
ACACCTTGCA TGCTGCGAGA TTCTGACAGC ATATTGGAGA CCCTTCAGAG AAAGCTTGGA 
ATAAAGGTTG GAGAGACTAC ACCTGACAAA CTTTTCACTC TTATAGAAGT GGAATGTTTA 
GGGGCCTGTG TAAATGCACC GATGGTTCAA ATAAATGACA ACTACTATGA GGATCTGACA 
CCCAAGGATA TTGAAGAGAT TATTGATGAA CTCAAAGCTG GAAAAGTTCC CAAACCAGGG 
CCAAGGAGTG GCCGCTTCTG TTGTGAGCCA GCTGGAGGCC TTACTTCTTT GACTGAACCA 
CCCAAAGGAC CTGGCTTTGG TGTGCAAGCA GGCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NDUFV2 regulates neuronal migration in the developing cerebral cortex through modulation of the multipolar-bipolar transition.
Brain research1625102-10(2015 Nov)
Chen T,Wu Q,Zhang Y,Zhang D

Subunit-specific incorporation efficiency and kinetics in mitochondrial complex I homeostasis.
The Journal of biological chemistry287(50)41851-60(2012 Dec)
Dieteren CE,Koopman WJ,Swarts HG,Peters JG,Maczuga P,van Gemst JJ,Masereeuw R,Smeitink JA,Nijtmans LG,Willems PH

Molecular base of biochemical complex I deficiency.
Mitochondrion12(5)520-32(2012 Sep)
Hoefs SJ,Rodenburg RJ,Smeitink JA,van den Heuvel LP

Region-specific expression of mitochondrial complex I genes during murine brain development.
PloS one6(4)e18897(2011 Apr)
Wirtz S,Schuelke M

Proteolipid protein is required for transport of sirtuin 2 into CNS myelin.
The Journal of neuroscience : the official journal of the Society for Neuroscience27(29)7717-30(2007 Jul)
Werner HB,Kuhlmann K,Shen S,Uecker M,Schardt A,Dimova K,Orfaniotou F,Dhaunchak A,Brinkmann BG,M?bius W,Guarente L,Casaccia-Bonnefil P,Jahn O,Nave KA

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Ndufv2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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