Rint1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rint1
Entrez Gene ID	72772
Full Name	RAD50 interactor 1
Synonyms	1500019C06Rik,2810450M21Rik,Rint-1
General protein information	Preferred Names RAD50 interactor 1 Names RAD50-interacting protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_177323.4	NP_796297.2	RAD50-interacting protein 1 isoform 1
NM_001310467.1	NP_001297396.1	RAD50-interacting protein 1 isoform 2
XM_030254833.1	XP_030110693.1	RAD50-interacting protein 1 isoform X1
XM_006535810.3	XP_006535873.1	RAD50-interacting protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RINT1	XP_001146982.2
Bos taurus (cattle)	RINT1	NP_001070297.1
Danio rerio (zebrafish)	rint1	NP_001038468.2
Homo sapiens (human)	RINT1	NP_068749.3
	RINT1	XP_002803466.1
Canis lupus familiaris (dog)	RINT1	XP_005631022.1
Mus musculus (house mouse)	Rint1	NP_796297.2
Neurospora crassa	NCU02270	XP_959914.1
Xenopus tropicalis (tropical clawed frog)	rint1	XP_002933153.2
Rattus norvegicus (Norway rat)	Rint1	XP_006236037.1
Gallus gallus (chicken)	RINT1	NP_001265046.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007482	XP_308391.3
Magnaporthe oryzae (rice blast fungus)	MGG_00918	XP_003718042.1
Drosophila melanogaster (fruit fly)	CG8605	NP_648102.2

Gene Ontology
Bibliography

Related articles in PubMed

Evaluation of Rint1 as a modifier of intestinal tumorigenesis and cancer risk.
Otterpohl KL, Gould KA
PloS one12(3)e0172247(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

RINT1 functions as a multitasking protein at the crossroads between genomic stability, ER homeostasis, and autophagy.
Grigaravicius P, von Deimling A, Frappart PO
Autophagy12(8)1413-5(2016 Aug)

Rint1 inactivation triggers genomic instability, ER stress and autophagy inhibition in the brain.
Grigaravicius P, Kaminska E, H?bner CA, McKinnon PJ, von Deimling A, Frappart PO
Cell death and differentiation23(3)454-68(2016 Mar)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these data are more consistent with the hypothesis that RINT1 functions as an oncogene rather than a tumor suppressor gene in the context of colorectal cancer.
Title: Evaluation of Rint1 as a modifier of intestinal tumorigenesis and cancer risk.

RINT1 has a role as a multitasking protein with roles in genomic stability, ER homeostasis, and autophagy
Title: RINT1 functions as a multitasking protein at the crossroads between genomic stability, ER homeostasis, and autophagy.

A crucial role of Rint1 in vivo in genomic stability maintenance, as well as in prevention of endoplasmic reticulum stress and autophagy.
Title: Rint1 inactivation triggers genomic instability, ER stress and autophagy inhibition in the brain.

These findings suggest that RINT-1 serves as a novel tumor suppressor essential for maintaining the dynamic integrity of the Golgi apparatus and the centrosome, a prerequisite to their proper coordination during cell division.
Title: RINT-1 serves as a tumor suppressor and maintains Golgi dynamics and centrosome integrity for cell survival.

The following Rint1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rint1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu34856	XM_006535810.3 Latest version!	Mus musculus RAD50 interactor 1 (Rint1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu70951	NM_001310467.1 Latest version!	Mus musculus RAD50 interactor 1 (Rint1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu06286	NM_177323.4 Latest version!	Mus musculus RAD50 interactor 1 (Rint1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$468.30 $669.00
OMu34856	XM_030254833.1 Latest version!	Mus musculus RAD50 interactor 1 (Rint1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rint1 ( XM_006535810.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535873.1 Mus musculus RAD50 interactor 1 (Rint1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34856
Clone ID Related Accession (Same CDS sequence)	XM_030254833.1 , XM_006535810.3
Accession Version	XM_006535810.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	RAD50-interacting protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535810.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CCTGGATGGA TGACCTTGGA GCCATGATCA CCCAGATGGA AGAGATTGAG 
CGGCATCTCG CATACCTTAA GTGGGTGTCG CAGACTGAGG AGCTGAGTGA TAACATTCAG 
CAGTACCTGA TGACCAACAG TGTGCCAGAG GCAGCCTCTC TCCTGGTGAC CATGACAGAA 
CTGGACATCC AGCTTCAGGA GTCATCCTGT ACTCATCTTC TTAGTTTCAT GAGAGCCACA 
GTTAAATTCT GGCATAAAAT TCTCAAGGAC AAGCTTACCA GTGATTTTGA GGAAGTCTTG 
GCACAGCTGC ATTGGCCATT CACCTCACAT ACCCAGTCGC AAACCGTTGG CGGGAGCCGG 
CCTGCCGGTA CCCCGGAGCT GTACAGTAGC CTGGACACAC TGTTTTGTCA GCTGTTGAAA 
CTACAGGCCT CAGATGAGTT ATTAACTGAG CCAAAACAAC TCCCAGAAAA ATACTGTCTT 
CCTGCCTCCC CTCCCGTGAC CCTGCCCATA CAGGTCATGC TGGCTCCCCT GCAGAAGAGA 
TTCAGGTACC ACTTCAGAGG CAGCCGGCAG ACCAACGTCA TGAGCAAGCC TGAATGGTAC 
TTGGCTCAGG TACTTATGTG GATTGGAAAC CATACACAGT TTCTGGATGA GAAGATTCAG 
CCAATATTGG ACAAAGTGGG CTCTGCAGTA AATGCAAGGC TTGAGTTTTC CCGTGGCCTT 
GTGATGCTCA TTCTTGAGAA GCTGGCTTCT GATATCCCCT GCCTGCTCTA CGATGACAGC 
CTCTTCTGTC ACCTGGTGGA TGAGGTGCTG CTGTTTGAAA GGGAGCTGCA CACCGTTCAC 
GGCTATCCCA GCACATTCGC CAGCTGCATG CACATCCTGT CGGAGGAGAC CTGTTTCCAG 
AGATGGCTGA CTGTGGAGAG AAAGTTTGCT CTTCAAAAAA TGGACTCAAT GCTTTCGTCA 
GAGGCTGCCT GGGTATCCCA GTATAAAGAC ATCTCTGATG TGGATGAAAT GAAGGTTCCA 
GACTGTGCGG AAGTCTTTAT GACCCTGCTT TTGGTTATAA CTGACAGGTA TAAAAATCTC 
CCCACAGCCT CCCGGAAACT GCAGTTCCTG GAGCTACAGA AGGACCTGGT AGATGATTTC 
AGGATCCGGT TAACGCAGGT GATGAAGGAG GAGACGAGAG CGGCCCTGGG CTTTCGGTAC 
TGTGCGATTC TCAATGCCGT GAACTATATC TCAGCAGTGC TGGCAGACTG GGCTGACAAT 
GTTTTCTTTC TGCAGCTGCA ACAGGCAGCA CTGGAGGTCT TTGCAGAGAA TAACGTCCTC 
AGTAAACTGC AGCTGGGACA GCTGGCCTCC ATGGAGAGCT CGGTCTTCGA TGACATGATC 
AACCTCTTAG AGCGTTTGAA GCTTGATATG TTGACCCGCC AAGTAGACCA TGTTTTCAGA 
GAAGTGAAAG ACTCTGCAAA GTTGTATAAG AAAGAAAGAT GGTTGTCCCT GCCGTCTCAG 
TCGGAACAGG CAGTGATGTC GCTGTCCAGC TCAGCCTGCC CGCTCCTGCT GACCTTACGG 
GACCGTCTGC TCCAGCTGGA GCAGCAGCTG TGTTTCTCGC TATTCAGAAT CTTCTGGCAA 
ATGCTGGCAG AGAAGCTGGA CCTGTACATC TACCAGGAAA TAATCCTTGC TAACCACTTC 
AACGAAGGAG GAGCAGCCCA GCTGCAGTTT GATATGACAA GGAACCTCTT TCCTTTGTTT 
TCTCACTATT GCAAGAGACC AGAAAACTAT TTTAAACATG TAAAAGAAGC CTGCATCATT 
CTGAATTTGA ACATCGGTTC TGCATTACTG CTGAAAGATG TCCTGCAGTC AGTGTCCGAG 
CACATTCCTG CCACAGCAGC CTTAAATGAA GTTGGGGTTT ACAAACTGGC TCAACAAGAC 
GTTGAAATTC TGCTTAATTT GAGAACAAAT TGGCCTAACA CTGGAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535873.1
CDS	179..2149
Translation	MAQPWMDDLGAMITQMEEIERHLAYLKWVSQTEELSDNIQQYLMTNSVPEAASLLVTMTELDIQLQESSCTHLLSFMRATVKFWHKILKDKLTSDFEEVLAQLHWPFTSHTQSQTVGGSRPAGTPELYSSLDTLFCQLLKLQASDELLTEPKQLPEKYCLPASPPVTLPIQVMLAPLQKRFRYHFRGSRQTNVMSKPEWYLAQVLMWIGNHTQFLDEKIQPILDKVGSAVNARLEFSRGLVMLILEKLASDIPCLLYDDSLFCHLVDEVLLFERELHTVHGYPSTFASCMHILSEETCFQRWLTVERKFALQKMDSMLSSEAAWVSQYKDISDVDEMKVPDCAEVFMTLLLVITDRYKNLPTASRKLQFLELQKDLVDDFRIRLTQVMKEETRAALGFRYCAILNAVNYISAVLADWADNVFFLQLQQAALEVFAENNVLSKLQLGQLASMESSVFDDMINLLERLKLDMLTRQVDHVFREVKDSAKLYKKERWLSLPSQSEQAVMSLSSSACPLLLTLRDRLLQLEQQLCFSLFRIFWQMLAEKLDLYIYQEIILANHFNEGGAAQLQFDMTRNLFPLFSHYCKRPENYFKHVKEACIILNLNIGSALLLKDVLQSVSEHIPATAALNEVGVYKLAQQDVEILLNLRTNWPNTGK

Target ORF information:

RefSeq Version	XM_006535810.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAD50 interactor 1 (Rint1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535810.3

ORF Insert Sequence:

ATGGCTCAGC CCTGGATGGA TGACCTTGGA GCCATGATCA CCCAGATGGA AGAGATTGAG 
CGGCATCTCG CATACCTTAA GTGGGTGTCG CAGACTGAGG AGCTGAGTGA TAACATTCAG 
CAGTACCTGA TGACCAACAG TGTGCCAGAG GCAGCCTCTC TCCTGGTGAC CATGACAGAA 
CTGGACATCC AGCTTCAGGA GTCATCCTGT ACTCATCTTC TTAGTTTCAT GAGAGCCACA 
GTTAAATTCT GGCATAAAAT TCTCAAGGAC AAGCTTACCA GTGATTTTGA GGAAGTCTTG 
GCACAGCTGC ATTGGCCATT CACCTCACAT ACCCAGTCGC AAACCGTTGG CGGGAGCCGG 
CCTGCCGGTA CCCCGGAGCT GTACAGTAGC CTGGACACAC TGTTTTGTCA GCTGTTGAAA 
CTACAGGCCT CAGATGAGTT ATTAACTGAG CCAAAACAAC TCCCAGAAAA ATACTGTCTT 
CCTGCCTCCC CTCCCGTGAC CCTGCCCATA CAGGTCATGC TGGCTCCCCT GCAGAAGAGA 
TTCAGGTACC ACTTCAGAGG CAGCCGGCAG ACCAACGTCA TGAGCAAGCC TGAATGGTAC 
TTGGCTCAGG TACTTATGTG GATTGGAAAC CATACACAGT TTCTGGATGA GAAGATTCAG 
CCAATATTGG ACAAAGTGGG CTCTGCAGTA AATGCAAGGC TTGAGTTTTC CCGTGGCCTT 
GTGATGCTCA TTCTTGAGAA GCTGGCTTCT GATATCCCCT GCCTGCTCTA CGATGACAGC 
CTCTTCTGTC ACCTGGTGGA TGAGGTGCTG CTGTTTGAAA GGGAGCTGCA CACCGTTCAC 
GGCTATCCCA GCACATTCGC CAGCTGCATG CACATCCTGT CGGAGGAGAC CTGTTTCCAG 
AGATGGCTGA CTGTGGAGAG AAAGTTTGCT CTTCAAAAAA TGGACTCAAT GCTTTCGTCA 
GAGGCTGCCT GGGTATCCCA GTATAAAGAC ATCTCTGATG TGGATGAAAT GAAGGTTCCA 
GACTGTGCGG AAGTCTTTAT GACCCTGCTT TTGGTTATAA CTGACAGGTA TAAAAATCTC 
CCCACAGCCT CCCGGAAACT GCAGTTCCTG GAGCTACAGA AGGACCTGGT AGATGATTTC 
AGGATCCGGT TAACGCAGGT GATGAAGGAG GAGACGAGAG CGGCCCTGGG CTTTCGGTAC 
TGTGCGATTC TCAATGCCGT GAACTATATC TCAGCAGTGC TGGCAGACTG GGCTGACAAT 
GTTTTCTTTC TGCAGCTGCA ACAGGCAGCA CTGGAGGTCT TTGCAGAGAA TAACGTCCTC 
AGTAAACTGC AGCTGGGACA GCTGGCCTCC ATGGAGAGCT CGGTCTTCGA TGACATGATC 
AACCTCTTAG AGCGTTTGAA GCTTGATATG TTGACCCGCC AAGTAGACCA TGTTTTCAGA 
GAAGTGAAAG ACTCTGCAAA GTTGTATAAG AAAGAAAGAT GGTTGTCCCT GCCGTCTCAG 
TCGGAACAGG CAGTGATGTC GCTGTCCAGC TCAGCCTGCC CGCTCCTGCT GACCTTACGG 
GACCGTCTGC TCCAGCTGGA GCAGCAGCTG TGTTTCTCGC TATTCAGAAT CTTCTGGCAA 
ATGCTGGCAG AGAAGCTGGA CCTGTACATC TACCAGGAAA TAATCCTTGC TAACCACTTC 
AACGAAGGAG GAGCAGCCCA GCTGCAGTTT GATATGACAA GGAACCTCTT TCCTTTGTTT 
TCTCACTATT GCAAGAGACC AGAAAACTAT TTTAAACATG TAAAAGAAGC CTGCATCATT 
CTGAATTTGA ACATCGGTTC TGCATTACTG CTGAAAGATG TCCTGCAGTC AGTGTCCGAG 
CACATTCCTG CCACAGCAGC CTTAAATGAA GTTGGGGTTT ACAAACTGGC TCAACAAGAC 
GTTGAAATTC TGCTTAATTT GAGAACAAAT TGGCCTAACA CTGGAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rint1 ( NM_001310467.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297396.1 Mus musculus RAD50 interactor 1 (Rint1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu70951
Clone ID Related Accession (Same CDS sequence)	NM_001310467.1
Accession Version	NM_001310467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	RAD50-interacting protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK005284.1, AK028707.1 and BC065059.1. On Jul 14, 2015 this sequence version replaced XM_006535809.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK028707.1, BC145267.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTGCGG CTGACGATAT CGGCGAAGTG CCGGCAGCCC CGTGCTGCCC CGAGAGTGGT 
GACGAAACGA AGAACACAGA CGTGAAAAGT GACGTGAACA CTGCAGCTCC TGCTGGAAGC 
GAGCAGCTCA GCCAAGGTGG CAGTGACGAC GCGCTCCTTT CCTATGTGTC TGCATTCATA 
GAGAAGGAAG TGGGAAGTGA CCTTAAGTCT TTGAAGACAC TTGGTAAACT CATAGAACAA 
ATGACAGAAA GCAAAGTGAA GTTAGAAGAA CAGGTACTTA CAATTTCATC AGAAATCCCT 
AAAAGAATTC AAAGTGCTTT AAAAGATGCA GAGGAATCCA AACAACTTCT TGATGAGTTT 
CTGGAGCAGG AAGCGCCTCT CTTCAGCTCC ATTAGCAGCC ATCTGCTGAT GGCTCAGCCC 
TGGATGGATG ACCTTGGAGC CATGATCACC CAGATGGAAG AGATTGAGCG GCATCTCGCA 
TACCTTAAGT GGGTGTCGCA GACTGAGGAG CTGAGTGATT TTGAGGAAGT CTTGGCACAG 
CTGCATTGGC CATTCACCTC ACATACCCAG TCGCAAACCG TTGGCGGGAG CCGGCCTGCC 
GGTACCCCGG AGCTGTACAG TAGCCTGGAC ACACTGTTTT GTCAGCTGTT GAAACTACAG 
GCCTCAGATG AGTTATTAAC TGAGCCAAAA CAACTCCCAG AAAAATACTG TCTTCCTGCC 
TCCCCTCCCG TGACCCTGCC CATACAGGTC ATGCTGGCTC CCCTGCAGAA GAGATTCAGG 
TACCACTTCA GAGGCAGCCG GCAGACCAAC GTCATGAGCA AGCCTGAATG GTACTTGGCT 
CAGGTACTTA TGTGGATTGG AAACCATACA CAGTTTCTGG ATGAGAAGAT TCAGCCAATA 
TTGGACAAAG TGGGCTCTGC AGTAAATGCA AGGCTTGAGT TTTCCCGTGG CCTTGTGATG 
CTCATTCTTG AGAAGCTGGC TTCTGATATC CCCTGCCTGC TCTACGATGA CAGCCTCTTC 
TGTCACCTGG TGGATGAGGT GCTGCTGTTT GAAAGGGAGC TGCACACCGT TCACGGCTAT 
CCCAGCACAT TCGCCAGCTG CATGCACATC CTGTCGGAGG AGACCTGTTT CCAGAGATGG 
CTGACTGTGG AGAGAAAGTT TGCTCTTCAA AAAATGGACT CAATGCTTTC GTCAGAGGCT 
GCCTGGGTAT CCCAGTATAA AGACATCTCT GATGTGGATG AAATGAAGGT TCCAGACTGT 
GCGGAAGTCT TTATGACCCT GCTTTTGGTT ATAACTGACA GGTATAAAAA TCTCCCCACA 
GCCTCCCGGA AACTGCAGTT CCTGGAGCTA CAGAAGGACC TGGTAGATGA TTTCAGGATC 
CGGTTAACGC AGGTGATGAA GGAGGAGACG AGAGCGGCCC TGGGCTTTCG GTACTGTGCG 
ATTCTCAATG CCGTGAACTA TATCTCAGCA GTGCTGGCAG ACTGGGCTGA CAATGTTTTC 
TTTCTGCAGC TGCAACAGGC AGCACTGGAG GTCTTTGCAG AGAATAACGT CCTCAGTAAA 
CTGCAGCTGG GACAGCTGGC CTCCATGGAG AGCTCGGTCT TCGATGACAT GATCAACCTC 
TTAGAGCGTT TGAAGCTTGA TATGTTGACC CGCCAAGTAG ACCATGTTTT CAGAGAAGTG 
AAAGACTCTG CAAAGTTGTA TAAGAAAGAA AGATGGTTGT CCCTGCCGTC TCAGTCGGAA 
CAGGCAGTGA TGTCGCTGTC CAGCTCAGCC TGCCCGCTCC TGCTGACCTT ACGGGACCGT 
CTGCTCCAGC TGGAGCAGCA GCTGTGTTTC TCGCTATTCA GAATCTTCTG GCAAATGCTG 
GCAGAGAAGC TGGACCTGTA CATCTACCAG GAAATAATCC TTGCTAACCA CTTCAACGAA 
GGAGGAGCAG CCCAGCTGCA GTTTGATATG ACAAGGAACC TCTTTCCTTT GTTTTCTCAC 
TATTGCAAGA GACCAGAAAA CTATTTTAAA CATGTAAAAG AAGCCTGCAT CATTCTGAAT 
TTGAACATCG GTTCTGCATT ACTGCTGAAA GATGTCCTGC AGTCAGTGTC CGAGCACATT 
CCTGCCACAG CAGCCTTAAA TGAAGTTGGG GTTTACAAAC TGGCTCAACA AGACGTTGAA 
ATTCTGCTTA ATTTGAGAAC AAATTGGCCT AACACTGGAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297396.1
CDS	131..2335
Translation	MLAADDIGEVPAAPCCPESGDETKNTDVKSDVNTAAPAGSEQLSQGGSDDALLSYVSAFIEKEVGSDLKSLKTLGKLIEQMTESKVKLEEQVLTISSEIPKRIQSALKDAEESKQLLDEFLEQEAPLFSSISSHLLMAQPWMDDLGAMITQMEEIERHLAYLKWVSQTEELSDFEEVLAQLHWPFTSHTQSQTVGGSRPAGTPELYSSLDTLFCQLLKLQASDELLTEPKQLPEKYCLPASPPVTLPIQVMLAPLQKRFRYHFRGSRQTNVMSKPEWYLAQVLMWIGNHTQFLDEKIQPILDKVGSAVNARLEFSRGLVMLILEKLASDIPCLLYDDSLFCHLVDEVLLFERELHTVHGYPSTFASCMHILSEETCFQRWLTVERKFALQKMDSMLSSEAAWVSQYKDISDVDEMKVPDCAEVFMTLLLVITDRYKNLPTASRKLQFLELQKDLVDDFRIRLTQVMKEETRAALGFRYCAILNAVNYISAVLADWADNVFFLQLQQAALEVFAENNVLSKLQLGQLASMESSVFDDMINLLERLKLDMLTRQVDHVFREVKDSAKLYKKERWLSLPSQSEQAVMSLSSSACPLLLTLRDRLLQLEQQLCFSLFRIFWQMLAEKLDLYIYQEIILANHFNEGGAAQLQFDMTRNLFPLFSHYCKRPENYFKHVKEACIILNLNIGSALLLKDVLQSVSEHIPATAALNEVGVYKLAQQDVEILLNLRTNWPNTGK

Target ORF information:

RefSeq Version	NM_001310467.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAD50 interactor 1 (Rint1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310467.1

ORF Insert Sequence:

ATGCTTGCGG CTGACGATAT CGGCGAAGTG CCGGCAGCCC CGTGCTGCCC CGAGAGTGGT 
GACGAAACGA AGAACACAGA CGTGAAAAGT GACGTGAACA CTGCAGCTCC TGCTGGAAGC 
GAGCAGCTCA GCCAAGGTGG CAGTGACGAC GCGCTCCTTT CCTATGTGTC TGCATTCATA 
GAGAAGGAAG TGGGAAGTGA CCTTAAGTCT TTGAAGACAC TTGGTAAACT CATAGAACAA 
ATGACAGAAA GCAAAGTGAA GTTAGAAGAA CAGGTACTTA CAATTTCATC AGAAATCCCT 
AAAAGAATTC AAAGTGCTTT AAAAGATGCA GAGGAATCCA AACAACTTCT TGATGAGTTT 
CTGGAGCAGG AAGCGCCTCT CTTCAGCTCC ATTAGCAGCC ATCTGCTGAT GGCTCAGCCC 
TGGATGGATG ACCTTGGAGC CATGATCACC CAGATGGAAG AGATTGAGCG GCATCTCGCA 
TACCTTAAGT GGGTGTCGCA GACTGAGGAG CTGAGTGATT TTGAGGAAGT CTTGGCACAG 
CTGCATTGGC CATTCACCTC ACATACCCAG TCGCAAACCG TTGGCGGGAG CCGGCCTGCC 
GGTACCCCGG AGCTGTACAG TAGCCTGGAC ACACTGTTTT GTCAGCTGTT GAAACTACAG 
GCCTCAGATG AGTTATTAAC TGAGCCAAAA CAACTCCCAG AAAAATACTG TCTTCCTGCC 
TCCCCTCCCG TGACCCTGCC CATACAGGTC ATGCTGGCTC CCCTGCAGAA GAGATTCAGG 
TACCACTTCA GAGGCAGCCG GCAGACCAAC GTCATGAGCA AGCCTGAATG GTACTTGGCT 
CAGGTACTTA TGTGGATTGG AAACCATACA CAGTTTCTGG ATGAGAAGAT TCAGCCAATA 
TTGGACAAAG TGGGCTCTGC AGTAAATGCA AGGCTTGAGT TTTCCCGTGG CCTTGTGATG 
CTCATTCTTG AGAAGCTGGC TTCTGATATC CCCTGCCTGC TCTACGATGA CAGCCTCTTC 
TGTCACCTGG TGGATGAGGT GCTGCTGTTT GAAAGGGAGC TGCACACCGT TCACGGCTAT 
CCCAGCACAT TCGCCAGCTG CATGCACATC CTGTCGGAGG AGACCTGTTT CCAGAGATGG 
CTGACTGTGG AGAGAAAGTT TGCTCTTCAA AAAATGGACT CAATGCTTTC GTCAGAGGCT 
GCCTGGGTAT CCCAGTATAA AGACATCTCT GATGTGGATG AAATGAAGGT TCCAGACTGT 
GCGGAAGTCT TTATGACCCT GCTTTTGGTT ATAACTGACA GGTATAAAAA TCTCCCCACA 
GCCTCCCGGA AACTGCAGTT CCTGGAGCTA CAGAAGGACC TGGTAGATGA TTTCAGGATC 
CGGTTAACGC AGGTGATGAA GGAGGAGACG AGAGCGGCCC TGGGCTTTCG GTACTGTGCG 
ATTCTCAATG CCGTGAACTA TATCTCAGCA GTGCTGGCAG ACTGGGCTGA CAATGTTTTC 
TTTCTGCAGC TGCAACAGGC AGCACTGGAG GTCTTTGCAG AGAATAACGT CCTCAGTAAA 
CTGCAGCTGG GACAGCTGGC CTCCATGGAG AGCTCGGTCT TCGATGACAT GATCAACCTC 
TTAGAGCGTT TGAAGCTTGA TATGTTGACC CGCCAAGTAG ACCATGTTTT CAGAGAAGTG 
AAAGACTCTG CAAAGTTGTA TAAGAAAGAA AGATGGTTGT CCCTGCCGTC TCAGTCGGAA 
CAGGCAGTGA TGTCGCTGTC CAGCTCAGCC TGCCCGCTCC TGCTGACCTT ACGGGACCGT 
CTGCTCCAGC TGGAGCAGCA GCTGTGTTTC TCGCTATTCA GAATCTTCTG GCAAATGCTG 
GCAGAGAAGC TGGACCTGTA CATCTACCAG GAAATAATCC TTGCTAACCA CTTCAACGAA 
GGAGGAGCAG CCCAGCTGCA GTTTGATATG ACAAGGAACC TCTTTCCTTT GTTTTCTCAC 
TATTGCAAGA GACCAGAAAA CTATTTTAAA CATGTAAAAG AAGCCTGCAT CATTCTGAAT 
TTGAACATCG GTTCTGCATT ACTGCTGAAA GATGTCCTGC AGTCAGTGTC CGAGCACATT 
CCTGCCACAG CAGCCTTAAA TGAAGTTGGG GTTTACAAAC TGGCTCAACA AGACGTTGAA 
ATTCTGCTTA ATTTGAGAAC AAATTGGCCT AACACTGGAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rint1 ( NM_177323.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796297.2 Mus musculus RAD50 interactor 1 (Rint1), transcript variant 1, mRNA.

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CloneID	OMu06286
Clone ID Related Accession (Same CDS sequence)	NM_177323.4
Accession Version	NM_177323.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	RAD50-interacting protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK005284.1, AK135334.1 and BC065059.1. On Jul 14, 2015 this sequence version replaced NM_177323.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC065059.1, SRR1660821.160574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTGCGG CTGACGATAT CGGCGAAGTG CCGGCAGCCC CGTGCTGCCC CGAGAGTGGT 
GACGAAACGA AGAACACAGA CGTGAAAAGT GACGTGAACA CTGCAGCTCC TGCTGGAAGC 
GAGCAGCTCA GCCAAGGTGG CAGTGACGAC GCGCTCCTTT CCTATGTGTC TGCATTCATA 
GAGAAGGAAG TGGGAAGTGA CCTTAAGTCT TTGAAGACAC TTGGTAAACT CATAGAACAA 
ATGACAGAAA GCAAAGTGAA GTTAGAAGAA CAGGTACTTA CAATTTCATC AGAAATCCCT 
AAAAGAATTC AAAGTGCTTT AAAAGATGCA GAGGAATCCA AACAACTTCT TGATGAGTTT 
CTGGAGCAGG AAGCGCCTCT CTTCAGCTCC ATTAGCAGCC ATCTGCTGAT GGCTCAGCCC 
TGGATGGATG ACCTTGGAGC CATGATCACC CAGATGGAAG AGATTGAGCG GCATCTCGCA 
TACCTTAAGT GGGTGTCGCA GACTGAGGAG CTGAGTGATA ACATTCAGCA GTACCTGATG 
ACCAACAGTG TGCCAGAGGC AGCCTCTCTC CTGGTGACCA TGACAGAACT GGACATCCAG 
CTTCAGGAGT CATCCTGTAC TCATCTTCTT AGTTTCATGA GAGCCACAGT TAAATTCTGG 
CATAAAATTC TCAAGGACAA GCTTACCAGT GATTTTGAGG AAGTCTTGGC ACAGCTGCAT 
TGGCCATTCA CCTCACATAC CCAGTCGCAA ACCGTTGGCG GGAGCCGGCC TGCCGGTACC 
CCGGAGCTGT ACAGTAGCCT GGACACACTG TTTTGTCAGC TGTTGAAACT ACAGGCCTCA 
GATGAGTTAT TAACTGAGCC AAAACAACTC CCAGAAAAAT ACTGTCTTCC TGCCTCCCCT 
CCCGTGACCC TGCCCATACA GGTCATGCTG GCTCCCCTGC AGAAGAGATT CAGGTACCAC 
TTCAGAGGCA GCCGGCAGAC CAACGTCATG AGCAAGCCTG AATGGTACTT GGCTCAGGTA 
CTTATGTGGA TTGGAAACCA TACACAGTTT CTGGATGAGA AGATTCAGCC AATATTGGAC 
AAAGTGGGCT CTGCAGTAAA TGCAAGGCTT GAGTTTTCCC GTGGCCTTGT GATGCTCATT 
CTTGAGAAGC TGGCTTCTGA TATCCCCTGC CTGCTCTACG ATGACAGCCT CTTCTGTCAC 
CTGGTGGATG AGGTGCTGCT GTTTGAAAGG GAGCTGCACA CCGTTCACGG CTATCCCAGC 
ACATTCGCCA GCTGCATGCA CATCCTGTCG GAGGAGACCT GTTTCCAGAG ATGGCTGACT 
GTGGAGAGAA AGTTTGCTCT TCAAAAAATG GACTCAATGC TTTCGTCAGA GGCTGCCTGG 
GTATCCCAGT ATAAAGACAT CTCTGATGTG GATGAAATGA AGGTTCCAGA CTGTGCGGAA 
GTCTTTATGA CCCTGCTTTT GGTTATAACT GACAGGTATA AAAATCTCCC CACAGCCTCC 
CGGAAACTGC AGTTCCTGGA GCTACAGAAG GACCTGGTAG ATGATTTCAG GATCCGGTTA 
ACGCAGGTGA TGAAGGAGGA GACGAGAGCG GCCCTGGGCT TTCGGTACTG TGCGATTCTC 
AATGCCGTGA ACTATATCTC AGCAGTGCTG GCAGACTGGG CTGACAATGT TTTCTTTCTG 
CAGCTGCAAC AGGCAGCACT GGAGGTCTTT GCAGAGAATA ACGTCCTCAG TAAACTGCAG 
CTGGGACAGC TGGCCTCCAT GGAGAGCTCG GTCTTCGATG ACATGATCAA CCTCTTAGAG 
CGTTTGAAGC TTGATATGTT GACCCGCCAA GTAGACCATG TTTTCAGAGA AGTGAAAGAC 
TCTGCAAAGT TGTATAAGAA AGAAAGATGG TTGTCCCTGC CGTCTCAGTC GGAACAGGCA 
GTGATGTCGC TGTCCAGCTC AGCCTGCCCG CTCCTGCTGA CCTTACGGGA CCGTCTGCTC 
CAGCTGGAGC AGCAGCTGTG TTTCTCGCTA TTCAGAATCT TCTGGCAAAT GCTGGCAGAG 
AAGCTGGACC TGTACATCTA CCAGGAAATA ATCCTTGCTA ACCACTTCAA CGAAGGAGGA 
GCAGCCCAGC TGCAGTTTGA TATGACAAGG AACCTCTTTC CTTTGTTTTC TCACTATTGC 
AAGAGACCAG AAAACTATTT TAAACATGTA AAAGAAGCCT GCATCATTCT GAATTTGAAC 
ATCGGTTCTG CATTACTGCT GAAAGATGTC CTGCAGTCAG TGTCCGAGCA CATTCCTGCC 
ACAGCAGCCT TAAATGAAGT TGGGGTTTAC AAACTGGCTC AACAAGACGT TGAAATTCTG 
CTTAATTTGA GAACAAATTG GCCTAACACT GGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796297.2
CDS	131..2509
Translation	MLAADDIGEVPAAPCCPESGDETKNTDVKSDVNTAAPAGSEQLSQGGSDDALLSYVSAFIEKEVGSDLKSLKTLGKLIEQMTESKVKLEEQVLTISSEIPKRIQSALKDAEESKQLLDEFLEQEAPLFSSISSHLLMAQPWMDDLGAMITQMEEIERHLAYLKWVSQTEELSDNIQQYLMTNSVPEAASLLVTMTELDIQLQESSCTHLLSFMRATVKFWHKILKDKLTSDFEEVLAQLHWPFTSHTQSQTVGGSRPAGTPELYSSLDTLFCQLLKLQASDELLTEPKQLPEKYCLPASPPVTLPIQVMLAPLQKRFRYHFRGSRQTNVMSKPEWYLAQVLMWIGNHTQFLDEKIQPILDKVGSAVNARLEFSRGLVMLILEKLASDIPCLLYDDSLFCHLVDEVLLFERELHTVHGYPSTFASCMHILSEETCFQRWLTVERKFALQKMDSMLSSEAAWVSQYKDISDVDEMKVPDCAEVFMTLLLVITDRYKNLPTASRKLQFLELQKDLVDDFRIRLTQVMKEETRAALGFRYCAILNAVNYISAVLADWADNVFFLQLQQAALEVFAENNVLSKLQLGQLASMESSVFDDMINLLERLKLDMLTRQVDHVFREVKDSAKLYKKERWLSLPSQSEQAVMSLSSSACPLLLTLRDRLLQLEQQLCFSLFRIFWQMLAEKLDLYIYQEIILANHFNEGGAAQLQFDMTRNLFPLFSHYCKRPENYFKHVKEACIILNLNIGSALLLKDVLQSVSEHIPATAALNEVGVYKLAQQDVEILLNLRTNWPNTGK

Target ORF information:

RefSeq Version	NM_177323.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAD50 interactor 1 (Rint1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177323.4

ORF Insert Sequence:

ATGCTTGCGG CTGACGATAT CGGCGAAGTG CCGGCAGCCC CGTGCTGCCC CGAGAGTGGT 
GACGAAACGA AGAACACAGA CGTGAAAAGT GACGTGAACA CTGCAGCTCC TGCTGGAAGC 
GAGCAGCTCA GCCAAGGTGG CAGTGACGAC GCGCTCCTTT CCTATGTGTC TGCATTCATA 
GAGAAGGAAG TGGGAAGTGA CCTTAAGTCT TTGAAGACAC TTGGTAAACT CATAGAACAA 
ATGACAGAAA GCAAAGTGAA GTTAGAAGAA CAGGTACTTA CAATTTCATC AGAAATCCCT 
AAAAGAATTC AAAGTGCTTT AAAAGATGCA GAGGAATCCA AACAACTTCT TGATGAGTTT 
CTGGAGCAGG AAGCGCCTCT CTTCAGCTCC ATTAGCAGCC ATCTGCTGAT GGCTCAGCCC 
TGGATGGATG ACCTTGGAGC CATGATCACC CAGATGGAAG AGATTGAGCG GCATCTCGCA 
TACCTTAAGT GGGTGTCGCA GACTGAGGAG CTGAGTGATA ACATTCAGCA GTACCTGATG 
ACCAACAGTG TGCCAGAGGC AGCCTCTCTC CTGGTGACCA TGACAGAACT GGACATCCAG 
CTTCAGGAGT CATCCTGTAC TCATCTTCTT AGTTTCATGA GAGCCACAGT TAAATTCTGG 
CATAAAATTC TCAAGGACAA GCTTACCAGT GATTTTGAGG AAGTCTTGGC ACAGCTGCAT 
TGGCCATTCA CCTCACATAC CCAGTCGCAA ACCGTTGGCG GGAGCCGGCC TGCCGGTACC 
CCGGAGCTGT ACAGTAGCCT GGACACACTG TTTTGTCAGC TGTTGAAACT ACAGGCCTCA 
GATGAGTTAT TAACTGAGCC AAAACAACTC CCAGAAAAAT ACTGTCTTCC TGCCTCCCCT 
CCCGTGACCC TGCCCATACA GGTCATGCTG GCTCCCCTGC AGAAGAGATT CAGGTACCAC 
TTCAGAGGCA GCCGGCAGAC CAACGTCATG AGCAAGCCTG AATGGTACTT GGCTCAGGTA 
CTTATGTGGA TTGGAAACCA TACACAGTTT CTGGATGAGA AGATTCAGCC AATATTGGAC 
AAAGTGGGCT CTGCAGTAAA TGCAAGGCTT GAGTTTTCCC GTGGCCTTGT GATGCTCATT 
CTTGAGAAGC TGGCTTCTGA TATCCCCTGC CTGCTCTACG ATGACAGCCT CTTCTGTCAC 
CTGGTGGATG AGGTGCTGCT GTTTGAAAGG GAGCTGCACA CCGTTCACGG CTATCCCAGC 
ACATTCGCCA GCTGCATGCA CATCCTGTCG GAGGAGACCT GTTTCCAGAG ATGGCTGACT 
GTGGAGAGAA AGTTTGCTCT TCAAAAAATG GACTCAATGC TTTCGTCAGA GGCTGCCTGG 
GTATCCCAGT ATAAAGACAT CTCTGATGTG GATGAAATGA AGGTTCCAGA CTGTGCGGAA 
GTCTTTATGA CCCTGCTTTT GGTTATAACT GACAGGTATA AAAATCTCCC CACAGCCTCC 
CGGAAACTGC AGTTCCTGGA GCTACAGAAG GACCTGGTAG ATGATTTCAG GATCCGGTTA 
ACGCAGGTGA TGAAGGAGGA GACGAGAGCG GCCCTGGGCT TTCGGTACTG TGCGATTCTC 
AATGCCGTGA ACTATATCTC AGCAGTGCTG GCAGACTGGG CTGACAATGT TTTCTTTCTG 
CAGCTGCAAC AGGCAGCACT GGAGGTCTTT GCAGAGAATA ACGTCCTCAG TAAACTGCAG 
CTGGGACAGC TGGCCTCCAT GGAGAGCTCG GTCTTCGATG ACATGATCAA CCTCTTAGAG 
CGTTTGAAGC TTGATATGTT GACCCGCCAA GTAGACCATG TTTTCAGAGA AGTGAAAGAC 
TCTGCAAAGT TGTATAAGAA AGAAAGATGG TTGTCCCTGC CGTCTCAGTC GGAACAGGCA 
GTGATGTCGC TGTCCAGCTC AGCCTGCCCG CTCCTGCTGA CCTTACGGGA CCGTCTGCTC 
CAGCTGGAGC AGCAGCTGTG TTTCTCGCTA TTCAGAATCT TCTGGCAAAT GCTGGCAGAG 
AAGCTGGACC TGTACATCTA CCAGGAAATA ATCCTTGCTA ACCACTTCAA CGAAGGAGGA 
GCAGCCCAGC TGCAGTTTGA TATGACAAGG AACCTCTTTC CTTTGTTTTC TCACTATTGC 
AAGAGACCAG AAAACTATTT TAAACATGTA AAAGAAGCCT GCATCATTCT GAATTTGAAC 
ATCGGTTCTG CATTACTGCT GAAAGATGTC CTGCAGTCAG TGTCCGAGCA CATTCCTGCC 
ACAGCAGCCT TAAATGAAGT TGGGGTTTAC AAACTGGCTC AACAAGACGT TGAAATTCTG 
CTTAATTTGA GAACAAATTG GCCTAACACT GGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rint1 ( XM_030254833.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110693.1 Mus musculus RAD50 interactor 1 (Rint1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34856
Clone ID Related Accession (Same CDS sequence)	XM_030254833.1 , XM_006535810.3
Accession Version	XM_030254833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	RAD50-interacting protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC CCTGGATGGA TGACCTTGGA GCCATGATCA CCCAGATGGA AGAGATTGAG 
CGGCATCTCG CATACCTTAA GTGGGTGTCG CAGACTGAGG AGCTGAGTGA TAACATTCAG 
CAGTACCTGA TGACCAACAG TGTGCCAGAG GCAGCCTCTC TCCTGGTGAC CATGACAGAA 
CTGGACATCC AGCTTCAGGA GTCATCCTGT ACTCATCTTC TTAGTTTCAT GAGAGCCACA 
GTTAAATTCT GGCATAAAAT TCTCAAGGAC AAGCTTACCA GTGATTTTGA GGAAGTCTTG 
GCACAGCTGC ATTGGCCATT CACCTCACAT ACCCAGTCGC AAACCGTTGG CGGGAGCCGG 
CCTGCCGGTA CCCCGGAGCT GTACAGTAGC CTGGACACAC TGTTTTGTCA GCTGTTGAAA 
CTACAGGCCT CAGATGAGTT ATTAACTGAG CCAAAACAAC TCCCAGAAAA ATACTGTCTT 
CCTGCCTCCC CTCCCGTGAC CCTGCCCATA CAGGTCATGC TGGCTCCCCT GCAGAAGAGA 
TTCAGGTACC ACTTCAGAGG CAGCCGGCAG ACCAACGTCA TGAGCAAGCC TGAATGGTAC 
TTGGCTCAGG TACTTATGTG GATTGGAAAC CATACACAGT TTCTGGATGA GAAGATTCAG 
CCAATATTGG ACAAAGTGGG CTCTGCAGTA AATGCAAGGC TTGAGTTTTC CCGTGGCCTT 
GTGATGCTCA TTCTTGAGAA GCTGGCTTCT GATATCCCCT GCCTGCTCTA CGATGACAGC 
CTCTTCTGTC ACCTGGTGGA TGAGGTGCTG CTGTTTGAAA GGGAGCTGCA CACCGTTCAC 
GGCTATCCCA GCACATTCGC CAGCTGCATG CACATCCTGT CGGAGGAGAC CTGTTTCCAG 
AGATGGCTGA CTGTGGAGAG AAAGTTTGCT CTTCAAAAAA TGGACTCAAT GCTTTCGTCA 
GAGGCTGCCT GGGTATCCCA GTATAAAGAC ATCTCTGATG TGGATGAAAT GAAGGTTCCA 
GACTGTGCGG AAGTCTTTAT GACCCTGCTT TTGGTTATAA CTGACAGGTA TAAAAATCTC 
CCCACAGCCT CCCGGAAACT GCAGTTCCTG GAGCTACAGA AGGACCTGGT AGATGATTTC 
AGGATCCGGT TAACGCAGGT GATGAAGGAG GAGACGAGAG CGGCCCTGGG CTTTCGGTAC 
TGTGCGATTC TCAATGCCGT GAACTATATC TCAGCAGTGC TGGCAGACTG GGCTGACAAT 
GTTTTCTTTC TGCAGCTGCA ACAGGCAGCA CTGGAGGTCT TTGCAGAGAA TAACGTCCTC 
AGTAAACTGC AGCTGGGACA GCTGGCCTCC ATGGAGAGCT CGGTCTTCGA TGACATGATC 
AACCTCTTAG AGCGTTTGAA GCTTGATATG TTGACCCGCC AAGTAGACCA TGTTTTCAGA 
GAAGTGAAAG ACTCTGCAAA GTTGTATAAG AAAGAAAGAT GGTTGTCCCT GCCGTCTCAG 
TCGGAACAGG CAGTGATGTC GCTGTCCAGC TCAGCCTGCC CGCTCCTGCT GACCTTACGG 
GACCGTCTGC TCCAGCTGGA GCAGCAGCTG TGTTTCTCGC TATTCAGAAT CTTCTGGCAA 
ATGCTGGCAG AGAAGCTGGA CCTGTACATC TACCAGGAAA TAATCCTTGC TAACCACTTC 
AACGAAGGAG GAGCAGCCCA GCTGCAGTTT GATATGACAA GGAACCTCTT TCCTTTGTTT 
TCTCACTATT GCAAGAGACC AGAAAACTAT TTTAAACATG TAAAAGAAGC CTGCATCATT 
CTGAATTTGA ACATCGGTTC TGCATTACTG CTGAAAGATG TCCTGCAGTC AGTGTCCGAG 
CACATTCCTG CCACAGCAGC CTTAAATGAA GTTGGGGTTT ACAAACTGGC TCAACAAGAC 
GTTGAAATTC TGCTTAATTT GAGAACAAAT TGGCCTAACA CTGGAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110693.1
CDS	367..2337
Translation	MAQPWMDDLGAMITQMEEIERHLAYLKWVSQTEELSDNIQQYLMTNSVPEAASLLVTMTELDIQLQESSCTHLLSFMRATVKFWHKILKDKLTSDFEEVLAQLHWPFTSHTQSQTVGGSRPAGTPELYSSLDTLFCQLLKLQASDELLTEPKQLPEKYCLPASPPVTLPIQVMLAPLQKRFRYHFRGSRQTNVMSKPEWYLAQVLMWIGNHTQFLDEKIQPILDKVGSAVNARLEFSRGLVMLILEKLASDIPCLLYDDSLFCHLVDEVLLFERELHTVHGYPSTFASCMHILSEETCFQRWLTVERKFALQKMDSMLSSEAAWVSQYKDISDVDEMKVPDCAEVFMTLLLVITDRYKNLPTASRKLQFLELQKDLVDDFRIRLTQVMKEETRAALGFRYCAILNAVNYISAVLADWADNVFFLQLQQAALEVFAENNVLSKLQLGQLASMESSVFDDMINLLERLKLDMLTRQVDHVFREVKDSAKLYKKERWLSLPSQSEQAVMSLSSSACPLLLTLRDRLLQLEQQLCFSLFRIFWQMLAEKLDLYIYQEIILANHFNEGGAAQLQFDMTRNLFPLFSHYCKRPENYFKHVKEACIILNLNIGSALLLKDVLQSVSEHIPATAALNEVGVYKLAQQDVEILLNLRTNWPNTGK

Target ORF information:

RefSeq Version	XM_030254833.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAD50 interactor 1 (Rint1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254833.1

ORF Insert Sequence:

ATGGCTCAGC CCTGGATGGA TGACCTTGGA GCCATGATCA CCCAGATGGA AGAGATTGAG 
CGGCATCTCG CATACCTTAA GTGGGTGTCG CAGACTGAGG AGCTGAGTGA TAACATTCAG 
CAGTACCTGA TGACCAACAG TGTGCCAGAG GCAGCCTCTC TCCTGGTGAC CATGACAGAA 
CTGGACATCC AGCTTCAGGA GTCATCCTGT ACTCATCTTC TTAGTTTCAT GAGAGCCACA 
GTTAAATTCT GGCATAAAAT TCTCAAGGAC AAGCTTACCA GTGATTTTGA GGAAGTCTTG 
GCACAGCTGC ATTGGCCATT CACCTCACAT ACCCAGTCGC AAACCGTTGG CGGGAGCCGG 
CCTGCCGGTA CCCCGGAGCT GTACAGTAGC CTGGACACAC TGTTTTGTCA GCTGTTGAAA 
CTACAGGCCT CAGATGAGTT ATTAACTGAG CCAAAACAAC TCCCAGAAAA ATACTGTCTT 
CCTGCCTCCC CTCCCGTGAC CCTGCCCATA CAGGTCATGC TGGCTCCCCT GCAGAAGAGA 
TTCAGGTACC ACTTCAGAGG CAGCCGGCAG ACCAACGTCA TGAGCAAGCC TGAATGGTAC 
TTGGCTCAGG TACTTATGTG GATTGGAAAC CATACACAGT TTCTGGATGA GAAGATTCAG 
CCAATATTGG ACAAAGTGGG CTCTGCAGTA AATGCAAGGC TTGAGTTTTC CCGTGGCCTT 
GTGATGCTCA TTCTTGAGAA GCTGGCTTCT GATATCCCCT GCCTGCTCTA CGATGACAGC 
CTCTTCTGTC ACCTGGTGGA TGAGGTGCTG CTGTTTGAAA GGGAGCTGCA CACCGTTCAC 
GGCTATCCCA GCACATTCGC CAGCTGCATG CACATCCTGT CGGAGGAGAC CTGTTTCCAG 
AGATGGCTGA CTGTGGAGAG AAAGTTTGCT CTTCAAAAAA TGGACTCAAT GCTTTCGTCA 
GAGGCTGCCT GGGTATCCCA GTATAAAGAC ATCTCTGATG TGGATGAAAT GAAGGTTCCA 
GACTGTGCGG AAGTCTTTAT GACCCTGCTT TTGGTTATAA CTGACAGGTA TAAAAATCTC 
CCCACAGCCT CCCGGAAACT GCAGTTCCTG GAGCTACAGA AGGACCTGGT AGATGATTTC 
AGGATCCGGT TAACGCAGGT GATGAAGGAG GAGACGAGAG CGGCCCTGGG CTTTCGGTAC 
TGTGCGATTC TCAATGCCGT GAACTATATC TCAGCAGTGC TGGCAGACTG GGCTGACAAT 
GTTTTCTTTC TGCAGCTGCA ACAGGCAGCA CTGGAGGTCT TTGCAGAGAA TAACGTCCTC 
AGTAAACTGC AGCTGGGACA GCTGGCCTCC ATGGAGAGCT CGGTCTTCGA TGACATGATC 
AACCTCTTAG AGCGTTTGAA GCTTGATATG TTGACCCGCC AAGTAGACCA TGTTTTCAGA 
GAAGTGAAAG ACTCTGCAAA GTTGTATAAG AAAGAAAGAT GGTTGTCCCT GCCGTCTCAG 
TCGGAACAGG CAGTGATGTC GCTGTCCAGC TCAGCCTGCC CGCTCCTGCT GACCTTACGG 
GACCGTCTGC TCCAGCTGGA GCAGCAGCTG TGTTTCTCGC TATTCAGAAT CTTCTGGCAA 
ATGCTGGCAG AGAAGCTGGA CCTGTACATC TACCAGGAAA TAATCCTTGC TAACCACTTC 
AACGAAGGAG GAGCAGCCCA GCTGCAGTTT GATATGACAA GGAACCTCTT TCCTTTGTTT 
TCTCACTATT GCAAGAGACC AGAAAACTAT TTTAAACATG TAAAAGAAGC CTGCATCATT 
CTGAATTTGA ACATCGGTTC TGCATTACTG CTGAAAGATG TCCTGCAGTC AGTGTCCGAG 
CACATTCCTG CCACAGCAGC CTTAAATGAA GTTGGGGTTT ACAAACTGGC TCAACAAGAC 
GTTGAAATTC TGCTTAATTT GAGAACAAAT TGGCCTAACA CTGGAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evaluation of Rint1 as a modifier of intestinal tumorigenesis and cancer risk.
PloS one12(3)e0172247(2017)
Otterpohl KL,Gould KA

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

RINT1 functions as a multitasking protein at the crossroads between genomic stability, ER homeostasis, and autophagy.
Autophagy12(8)1413-5(2016 Aug)
Grigaravicius P,von Deimling A,Frappart PO

Rint1 inactivation triggers genomic instability, ER stress and autophagy inhibition in the brain.
Cell death and differentiation23(3)454-68(2016 Mar)
Grigaravicius P,Kaminska E,H?bner CA,McKinnon PJ,von Deimling A,Frappart PO

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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Rint1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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