Riok1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Riok1
Entrez Gene ID	71340
Full Name	RIO kinase 1
Synonyms	3110046C13Rik,5430416A05Rik,Ad034
General protein information	Preferred Names RIO kinase 1 Names serine/threonine-protein kinase RIO1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A3.3
Summary	This gene encodes a member of the RIO family of atypical serine protein kinases. A similar protein in humans is a component of the protein arginine methyltransferase 5 complex that specifically recruits the RNA-binding protein nucleolin as a methylation substrate. [provided by RefSeq, Feb 2011].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017315606.1	XP_017171095.1	serine/threonine-protein kinase RIO1 isoform X1
XM_017315606.2	XP_017171095.1	serine/threonine-protein kinase RIO1 isoform X1
NM_024242.3	NP_077204.2	serine/threonine-protein kinase RIO1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RIOK1	XP_527225.2
Drosophila melanogaster (fruit fly)	CG11660	NP_648489.1
Kluyveromyces lactis	KLLA0E21121g	XP_454905.1
Gallus gallus (chicken)	RIOK1	XP_418958.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012121	XP_320408.4
Saccharomyces cerevisiae (baker's yeast)	RIO1	NP_014762.3
Eremothecium gossypii	AGOS_AER257W	NP_985114.1
Arabidopsis thaliana (thale cress)	AT5G37350	NP_851100.1
Schizosaccharomyces pombe (fission yeast)	SPAC10F6.10	NP_593261.2
Magnaporthe oryzae (rice blast fungus)	MGG_13447	XP_003711604.1
Xenopus tropicalis (tropical clawed frog)	riok1	XP_002932721.2
Homo sapiens (human)	RIOK1	NP_113668.2
Canis lupus familiaris (dog)	RIOK1	XP_535878.1
Bos taurus (cattle)	RIOK1	XP_002697645.1
Mus musculus (house mouse)	Riok1	NP_077204.2
Caenorhabditis elegans (roundworm)	riok-1	NP_001021570.2
Neurospora crassa	NCU10652	XP_956116.2
Arabidopsis thaliana (thale cress)	AT2G24990	NP_180071.1
	RIOK1	XP_001084661.1
Rattus norvegicus (Norway rat)	Riok1	NP_001093981.1
Danio rerio (zebrafish)	riok1	NP_998160.1
Oryza sativa (rice)	Os07g0162300	NP_001058946.1

Gene Ontology
Bibliography

Related articles in PubMed

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
Liu X, Jiao Y, Cao Y, Deng N, Ma Y, Hasty KA, Kang A, Chen H, Stuart JM, Gu W
BMC immunology17(1)25(2016 08)

RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity.
Guderian G, Peter C, Wiesner J, Sickmann A, Schulze-Osthoff K, Fischer U, Grimmler M
The Journal of biological chemistry286(3)1976-86(2011 Jan)

The RIO kinases: an atypical protein kinase family required for ribosome biogenesis and cell cycle progression.
LaRonde-LeBlanc N, Wlodawer A
Biochimica et biophysica acta1754(1-2)14-24(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Describes a function for human RIOK1 as a component of the protein arginine methyltransferase 5 complex, conferring specificity for nucleolin as a methylation substrate.
Title: RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity.

The following Riok1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Riok1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04583	NM_024242.3 Latest version!	Mus musculus RIO kinase 1 (Riok1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu48455	XM_017315606.2 Latest version!	Mus musculus RIO kinase 1 (Riok1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu48455	XM_017315606.1	Mus musculus RIO kinase 1 (yeast) (Riok1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Riok1 ( NM_024242.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077204.2 Mus musculus RIO kinase 1 (Riok1), mRNA.

Price & Availability↑

CloneID	OMu04583
Clone ID Related Accession (Same CDS sequence)	NM_024242.3
Accession Version	NM_024242.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase RIO1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CT010477.16, AK152707.1 and AV370421.1. On Feb 9, 2011 this sequence version replaced NM_024242.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK152707.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTGCA GTCTGGACAG GATGGCTTCG GTGGTCCCCG GGCAATTTGA CGACGCGGAT 
TCCTCAGACA GTGAAAACAA AGAACTGCAG CCAATCCATG CAGAAGATGG CGGCGTTCTA 
CTTAAAAGCC TCCAGAATGC TGCAGCAGAG GTGAAGGGCG ACGCTGAGAC CGATGAGGAA 
GACGACTATG ATGATGATGA TGATTGGTAC TTGGACGATG CAACTGGAAA ACTCACCAAG 
GGTTGTACCT GGAATGGCGG CAGTAACTAT CAGGCAAACC GACAGACTTC TAACTACAAT 
TCAGCCAAAA TGTCTACTCC AATTGACAAG TCCTTGCGAA AATTTGAAAA TAAAATCAAT 
CTAAATAAGC TGAATGTTAC TGATTCTGTG ACAAATAAAG TCACCGTAAA ATTGAGACAA 
AAGGAAGCAG AATCGTATCG AATTAAAGAT AAGGCCGACA GAGCAACCGT AGAACAGGTT 
TTGGATCCCA GAACAAGGAT GATTTTATTC AAGTTGTTGC ATAAAGACCA CATATCAGAG 
ATACATGGCT GTATTAGCAC TGGAAAAGAA GCTAATGTAT ACTATGCTAG CACACCCAGT 
GGAGAGAGCA GAGCAATCAA AATATACAAA ACTTCTATTT TGATGTTCAA AGATCGTGAT 
AAGTATGTGA CTGGGGAATT CAGGTTCCGG CGTGGCTATT GTAAAGGAAA CCCTAGGAAA 
ATGGTGAGGA CGTGGGCAGA GAAGGAGATG AGGAATTTGT GCAGGCTAAA AACAGCAAAC 
ATACCATGTC CAGAACCAAT CAGGCTAAGA AGTCATGTTC TTCTCATGGG CTTCATTGGC 
AAGGATGACA TGCCAGCCCC ACTTTTGAAA AATGTCCAGC TGTCAGAGTC CAAGGCACGG 
GAGTTGTACC TGCAGGTCAT TCAGTACATG AGGAAAATGT ATCAGGACGC TAGACTTGTC 
CACGCGGATC TCAGTGAATT CAACATGCTG TACCATGGTG GAGATGTTTA CATCATTGAT 
GTTTCTCAGT CTGTGGAGCA TGACCACCCA CATGCATTGG AGTTCTTGAG AAAAGACTGT 
ACCAATGTCA ATGATTTCTT TTCCAAGCAT GCTGTTGCAG TGATGACCGT GCGGGAGCTC 
TTCGACTTCG TCACAGATCC CTCCATCACT GCTGACAACA TGGATGCTTA CCTGGAAAAG 
GCTATGGAAA TAGCATCCCA GAGGACCAAG GAAGAAAAGA CTAGCCAAGA TCATGTGGAT 
GAAGAGGTGT TCAAACAAGC ATATATTCCC AGAACCTTAA ACGAAGTAAA GAATTATGAG 
AGAGATGTGG ACATCATGAT GAGGTTAAAG GAAGAAGACA TGGCTTTGAA CACTCAGCAA 
GACAACATTC TATACCAGAC TGTCATGGGA TTGAAAAAAG ATTTGTCAGG AGTCCAGAAG 
GTCCCCGCGC TCCTAGAAAG TGAAGTTAAG GAAGAGACTT GTTTTGGTTC AGACGATGCT 
GGGGGCTCTG AGTGCTCCGA CACAGTCTCT GAAGAGCAGG AAGATCAAGC CGGATGCAGA 
AACCATATTG CTGACCCTGA CATTGATAAA AAGGAAAGAA AAAAGATGGT CAAGGAAGCC 
CAGAGAGAGA AGAGAAAAAA TAAAATCCCT AAACATGTGA AAAAAAGAAA GGAAAAAACA 
GCCAAGGCCA AAAAAGGCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077204.2
CDS	473..2176
Translation	MDCSLDRMASVVPGQFDDADSSDSENKELQPIHAEDGGVLLKSLQNAAAEVKGDAETDEEDDYDDDDDWYLDDATGKLTKGCTWNGGSNYQANRQTSNYNSAKMSTPIDKSLRKFENKINLNKLNVTDSVTNKVTVKLRQKEAESYRIKDKADRATVEQVLDPRTRMILFKLLHKDHISEIHGCISTGKEANVYYASTPSGESRAIKIYKTSILMFKDRDKYVTGEFRFRRGYCKGNPRKMVRTWAEKEMRNLCRLKTANIPCPEPIRLRSHVLLMGFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRKMYQDARLVHADLSEFNMLYHGGDVYIIDVSQSVEHDHPHALEFLRKDCTNVNDFFSKHAVAVMTVRELFDFVTDPSITADNMDAYLEKAMEIASQRTKEEKTSQDHVDEEVFKQAYIPRTLNEVKNYERDVDIMMRLKEEDMALNTQQDNILYQTVMGLKKDLSGVQKVPALLESEVKEETCFGSDDAGGSECSDTVSEEQEDQAGCRNHIADPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKAKKGK

Target ORF information:

RefSeq Version	NM_024242.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RIO kinase 1 (Riok1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024242.3

ORF Insert Sequence:

ATGGACTGCA GTCTGGACAG GATGGCTTCG GTGGTCCCCG GGCAATTTGA CGACGCGGAT 
TCCTCAGACA GTGAAAACAA AGAACTGCAG CCAATCCATG CAGAAGATGG CGGCGTTCTA 
CTTAAAAGCC TCCAGAATGC TGCAGCAGAG GTGAAGGGCG ACGCTGAGAC CGATGAGGAA 
GACGACTATG ATGATGATGA TGATTGGTAC TTGGACGATG CAACTGGAAA ACTCACCAAG 
GGTTGTACCT GGAATGGCGG CAGTAACTAT CAGGCAAACC GACAGACTTC TAACTACAAT 
TCAGCCAAAA TGTCTACTCC AATTGACAAG TCCTTGCGAA AATTTGAAAA TAAAATCAAT 
CTAAATAAGC TGAATGTTAC TGATTCTGTG ACAAATAAAG TCACCGTAAA ATTGAGACAA 
AAGGAAGCAG AATCGTATCG AATTAAAGAT AAGGCCGACA GAGCAACCGT AGAACAGGTT 
TTGGATCCCA GAACAAGGAT GATTTTATTC AAGTTGTTGC ATAAAGACCA CATATCAGAG 
ATACATGGCT GTATTAGCAC TGGAAAAGAA GCTAATGTAT ACTATGCTAG CACACCCAGT 
GGAGAGAGCA GAGCAATCAA AATATACAAA ACTTCTATTT TGATGTTCAA AGATCGTGAT 
AAGTATGTGA CTGGGGAATT CAGGTTCCGG CGTGGCTATT GTAAAGGAAA CCCTAGGAAA 
ATGGTGAGGA CGTGGGCAGA GAAGGAGATG AGGAATTTGT GCAGGCTAAA AACAGCAAAC 
ATACCATGTC CAGAACCAAT CAGGCTAAGA AGTCATGTTC TTCTCATGGG CTTCATTGGC 
AAGGATGACA TGCCAGCCCC ACTTTTGAAA AATGTCCAGC TGTCAGAGTC CAAGGCACGG 
GAGTTGTACC TGCAGGTCAT TCAGTACATG AGGAAAATGT ATCAGGACGC TAGACTTGTC 
CACGCGGATC TCAGTGAATT CAACATGCTG TACCATGGTG GAGATGTTTA CATCATTGAT 
GTTTCTCAGT CTGTGGAGCA TGACCACCCA CATGCATTGG AGTTCTTGAG AAAAGACTGT 
ACCAATGTCA ATGATTTCTT TTCCAAGCAT GCTGTTGCAG TGATGACCGT GCGGGAGCTC 
TTCGACTTCG TCACAGATCC CTCCATCACT GCTGACAACA TGGATGCTTA CCTGGAAAAG 
GCTATGGAAA TAGCATCCCA GAGGACCAAG GAAGAAAAGA CTAGCCAAGA TCATGTGGAT 
GAAGAGGTGT TCAAACAAGC ATATATTCCC AGAACCTTAA ACGAAGTAAA GAATTATGAG 
AGAGATGTGG ACATCATGAT GAGGTTAAAG GAAGAAGACA TGGCTTTGAA CACTCAGCAA 
GACAACATTC TATACCAGAC TGTCATGGGA TTGAAAAAAG ATTTGTCAGG AGTCCAGAAG 
GTCCCCGCGC TCCTAGAAAG TGAAGTTAAG GAAGAGACTT GTTTTGGTTC AGACGATGCT 
GGGGGCTCTG AGTGCTCCGA CACAGTCTCT GAAGAGCAGG AAGATCAAGC CGGATGCAGA 
AACCATATTG CTGACCCTGA CATTGATAAA AAGGAAAGAA AAAAGATGGT CAAGGAAGCC 
CAGAGAGAGA AGAGAAAAAA TAAAATCCCT AAACATGTGA AAAAAAGAAA GGAAAAAACA 
GCCAAGGCCA AAAAAGGCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Riok1 ( XM_017315606.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171095.1 Mus musculus RIO kinase 1 (yeast) (Riok1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu48455
Clone ID Related Accession (Same CDS sequence)	XM_017315606.1 , XM_017315606.2
Accession Version	XM_017315606.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase RIO1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTAT TCAAGTTGTT GCATAAAGAC CACATATCAG AGATACATGG CTGTATTAGC 
ACTGGAAAAG AAGCTAATGT ATACTATGCT AGCACACCCA GTGGAGAGAG CAGAGCAATC 
AAAATATACA AAACTTCTAT TTTGATGTTC AAAGATCGTG ATAAGTATGT GACTGGGGAA 
TTCAGGTTCC GGCGTGGCTA TTGTAAAGGA AACCCTAGGA AAATGGTGAG GACGTGGGCA 
GAGAAGGAGA TGAGGAATTT GTGCAGGCTA AAAACAGCAA ACATACCATG TCCAGAACCA 
ATCAGGCTAA GAAGTCATGT TCTTCTCATG GGCTTCATTG GCAAGGATGA CATGCCAGCC 
CCACTTTTGA AAAATGTCCA GCTGTCAGAG TCCAAGGCAC GGGAGTTGTA CCTGCAGGTC 
ATTCAGTACA TGAGGAAAAT GTATCAGGAC GCTAGACTTG TCCACGCGGA TCTCAGTGAA 
TTCAACATGC TGTACCATGG TGGAGATGTT TACATCATTG ATGTTTCTCA GTCTGTGGAG 
CATGACCACC CACATGCATT GGAGTTCTTG AGAAAAGACT GTACCAATGT CAATGATTTC 
TTTTCCAAGC ATGCTGTTGC AGTGATGACC GTGCGGGAGC TCTTCGACTT CGTCACAGAT 
CCCTCCATCA CTGCTGACAA CATGGATGCT TACCTGGAAA AGGCTATGGA AATAGCATCC 
CAGAGGACCA AGGAAGAAAA GACTAGCCAA GATCATGTGG ATGAAGAGGT GTTCAAACAA 
GCATATATTC CCAGAACCTT AAACGAAGTA AAGAATTATG AGAGAGATGT GGACATCATG 
ATGAGGTTAA AGGAAGAAGA CATGGCTTTG AACACTCAGC AAGACAACAT TCTATACCAG 
ACTGTCATGG GATTGAAAAA AGATTTGTCA GGAGTCCAGA AGGTCCCCGC GCTCCTAGAA 
AGTGAAGTTA AGGAAGAGAC TTGTTTTGGT TCAGACGATG CTGGGGGCTC TGAGTGCTCC 
GACACAGTCT CTGAAGAGCA GGAAGATCAA GCCGGATGCA GAAACCATAT TGCTGACCCT 
GACATTGATA AAAAGGAAAG AAAAAAGATG GTCAAGGAAG CCCAGAGAGA GAAGAGAAAA 
AATAAAATCC CTAAACATGT GAAAAAAAGA AAGGAAAAAA CAGCCAAGGC CAAAAAAGGC 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171095.1
CDS	677..1882
Translation	MILFKLLHKDHISEIHGCISTGKEANVYYASTPSGESRAIKIYKTSILMFKDRDKYVTGEFRFRRGYCKGNPRKMVRTWAEKEMRNLCRLKTANIPCPEPIRLRSHVLLMGFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRKMYQDARLVHADLSEFNMLYHGGDVYIIDVSQSVEHDHPHALEFLRKDCTNVNDFFSKHAVAVMTVRELFDFVTDPSITADNMDAYLEKAMEIASQRTKEEKTSQDHVDEEVFKQAYIPRTLNEVKNYERDVDIMMRLKEEDMALNTQQDNILYQTVMGLKKDLSGVQKVPALLESEVKEETCFGSDDAGGSECSDTVSEEQEDQAGCRNHIADPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKAKKGK

Target ORF information:

RefSeq Version	XM_017315606.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RIO kinase 1 (yeast) (Riok1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315606.1

ORF Insert Sequence:

ATGATTTTAT TCAAGTTGTT GCATAAAGAC CACATATCAG AGATACATGG CTGTATTAGC 
ACTGGAAAAG AAGCTAATGT ATACTATGCT AGCACACCCA GTGGAGAGAG CAGAGCAATC 
AAAATATACA AAACTTCTAT TTTGATGTTC AAAGATCGTG ATAAGTATGT GACTGGGGAA 
TTCAGGTTCC GGCGTGGCTA TTGTAAAGGA AACCCTAGGA AAATGGTGAG GACGTGGGCA 
GAGAAGGAGA TGAGGAATTT GTGCAGGCTA AAAACAGCAA ACATACCATG TCCAGAACCA 
ATCAGGCTAA GAAGTCATGT TCTTCTCATG GGCTTCATTG GCAAGGATGA CATGCCAGCC 
CCACTTTTGA AAAATGTCCA GCTGTCAGAG TCCAAGGCAC GGGAGTTGTA CCTGCAGGTC 
ATTCAGTACA TGAGGAAAAT GTATCAGGAC GCTAGACTTG TCCACGCGGA TCTCAGTGAA 
TTCAACATGC TGTACCATGG TGGAGATGTT TACATCATTG ATGTTTCTCA GTCTGTGGAG 
CATGACCACC CACATGCATT GGAGTTCTTG AGAAAAGACT GTACCAATGT CAATGATTTC 
TTTTCCAAGC ATGCTGTTGC AGTGATGACC GTGCGGGAGC TCTTCGACTT CGTCACAGAT 
CCCTCCATCA CTGCTGACAA CATGGATGCT TACCTGGAAA AGGCTATGGA AATAGCATCC 
CAGAGGACCA AGGAAGAAAA GACTAGCCAA GATCATGTGG ATGAAGAGGT GTTCAAACAA 
GCATATATTC CCAGAACCTT AAACGAAGTA AAGAATTATG AGAGAGATGT GGACATCATG 
ATGAGGTTAA AGGAAGAAGA CATGGCTTTG AACACTCAGC AAGACAACAT TCTATACCAG 
ACTGTCATGG GATTGAAAAA AGATTTGTCA GGAGTCCAGA AGGTCCCCGC GCTCCTAGAA 
AGTGAAGTTA AGGAAGAGAC TTGTTTTGGT TCAGACGATG CTGGGGGCTC TGAGTGCTCC 
GACACAGTCT CTGAAGAGCA GGAAGATCAA GCCGGATGCA GAAACCATAT TGCTGACCCT 
GACATTGATA AAAAGGAAAG AAAAAAGATG GTCAAGGAAG CCCAGAGAGA GAAGAGAAAA 
AATAAAATCC CTAAACATGT GAAAAAAAGA AAGGAAAAAA CAGCCAAGGC CAAAAAAGGC 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Riok1 ( XM_017315606.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171095.1 Mus musculus RIO kinase 1 (Riok1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu48455
Clone ID Related Accession (Same CDS sequence)	XM_017315606.1 , XM_017315606.2
Accession Version	XM_017315606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase RIO1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTAT TCAAGTTGTT GCATAAAGAC CACATATCAG AGATACATGG CTGTATTAGC 
ACTGGAAAAG AAGCTAATGT ATACTATGCT AGCACACCCA GTGGAGAGAG CAGAGCAATC 
AAAATATACA AAACTTCTAT TTTGATGTTC AAAGATCGTG ATAAGTATGT GACTGGGGAA 
TTCAGGTTCC GGCGTGGCTA TTGTAAAGGA AACCCTAGGA AAATGGTGAG GACGTGGGCA 
GAGAAGGAGA TGAGGAATTT GTGCAGGCTA AAAACAGCAA ACATACCATG TCCAGAACCA 
ATCAGGCTAA GAAGTCATGT TCTTCTCATG GGCTTCATTG GCAAGGATGA CATGCCAGCC 
CCACTTTTGA AAAATGTCCA GCTGTCAGAG TCCAAGGCAC GGGAGTTGTA CCTGCAGGTC 
ATTCAGTACA TGAGGAAAAT GTATCAGGAC GCTAGACTTG TCCACGCGGA TCTCAGTGAA 
TTCAACATGC TGTACCATGG TGGAGATGTT TACATCATTG ATGTTTCTCA GTCTGTGGAG 
CATGACCACC CACATGCATT GGAGTTCTTG AGAAAAGACT GTACCAATGT CAATGATTTC 
TTTTCCAAGC ATGCTGTTGC AGTGATGACC GTGCGGGAGC TCTTCGACTT CGTCACAGAT 
CCCTCCATCA CTGCTGACAA CATGGATGCT TACCTGGAAA AGGCTATGGA AATAGCATCC 
CAGAGGACCA AGGAAGAAAA GACTAGCCAA GATCATGTGG ATGAAGAGGT GTTCAAACAA 
GCATATATTC CCAGAACCTT AAACGAAGTA AAGAATTATG AGAGAGATGT GGACATCATG 
ATGAGGTTAA AGGAAGAAGA CATGGCTTTG AACACTCAGC AAGACAACAT TCTATACCAG 
ACTGTCATGG GATTGAAAAA AGATTTGTCA GGAGTCCAGA AGGTCCCCGC GCTCCTAGAA 
AGTGAAGTTA AGGAAGAGAC TTGTTTTGGT TCAGACGATG CTGGGGGCTC TGAGTGCTCC 
GACACAGTCT CTGAAGAGCA GGAAGATCAA GCCGGATGCA GAAACCATAT TGCTGACCCT 
GACATTGATA AAAAGGAAAG AAAAAAGATG GTCAAGGAAG CCCAGAGAGA GAAGAGAAAA 
AATAAAATCC CTAAACATGT GAAAAAAAGA AAGGAAAAAA CAGCCAAGGC CAAAAAAGGC 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171095.1
CDS	678..1883
Translation	MILFKLLHKDHISEIHGCISTGKEANVYYASTPSGESRAIKIYKTSILMFKDRDKYVTGEFRFRRGYCKGNPRKMVRTWAEKEMRNLCRLKTANIPCPEPIRLRSHVLLMGFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRKMYQDARLVHADLSEFNMLYHGGDVYIIDVSQSVEHDHPHALEFLRKDCTNVNDFFSKHAVAVMTVRELFDFVTDPSITADNMDAYLEKAMEIASQRTKEEKTSQDHVDEEVFKQAYIPRTLNEVKNYERDVDIMMRLKEEDMALNTQQDNILYQTVMGLKKDLSGVQKVPALLESEVKEETCFGSDDAGGSECSDTVSEEQEDQAGCRNHIADPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKAKKGK

Target ORF information:

RefSeq Version	XM_017315606.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RIO kinase 1 (Riok1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315606.2

ORF Insert Sequence:

ATGATTTTAT TCAAGTTGTT GCATAAAGAC CACATATCAG AGATACATGG CTGTATTAGC 
ACTGGAAAAG AAGCTAATGT ATACTATGCT AGCACACCCA GTGGAGAGAG CAGAGCAATC 
AAAATATACA AAACTTCTAT TTTGATGTTC AAAGATCGTG ATAAGTATGT GACTGGGGAA 
TTCAGGTTCC GGCGTGGCTA TTGTAAAGGA AACCCTAGGA AAATGGTGAG GACGTGGGCA 
GAGAAGGAGA TGAGGAATTT GTGCAGGCTA AAAACAGCAA ACATACCATG TCCAGAACCA 
ATCAGGCTAA GAAGTCATGT TCTTCTCATG GGCTTCATTG GCAAGGATGA CATGCCAGCC 
CCACTTTTGA AAAATGTCCA GCTGTCAGAG TCCAAGGCAC GGGAGTTGTA CCTGCAGGTC 
ATTCAGTACA TGAGGAAAAT GTATCAGGAC GCTAGACTTG TCCACGCGGA TCTCAGTGAA 
TTCAACATGC TGTACCATGG TGGAGATGTT TACATCATTG ATGTTTCTCA GTCTGTGGAG 
CATGACCACC CACATGCATT GGAGTTCTTG AGAAAAGACT GTACCAATGT CAATGATTTC 
TTTTCCAAGC ATGCTGTTGC AGTGATGACC GTGCGGGAGC TCTTCGACTT CGTCACAGAT 
CCCTCCATCA CTGCTGACAA CATGGATGCT TACCTGGAAA AGGCTATGGA AATAGCATCC 
CAGAGGACCA AGGAAGAAAA GACTAGCCAA GATCATGTGG ATGAAGAGGT GTTCAAACAA 
GCATATATTC CCAGAACCTT AAACGAAGTA AAGAATTATG AGAGAGATGT GGACATCATG 
ATGAGGTTAA AGGAAGAAGA CATGGCTTTG AACACTCAGC AAGACAACAT TCTATACCAG 
ACTGTCATGG GATTGAAAAA AGATTTGTCA GGAGTCCAGA AGGTCCCCGC GCTCCTAGAA 
AGTGAAGTTA AGGAAGAGAC TTGTTTTGGT TCAGACGATG CTGGGGGCTC TGAGTGCTCC 
GACACAGTCT CTGAAGAGCA GGAAGATCAA GCCGGATGCA GAAACCATAT TGCTGACCCT 
GACATTGATA AAAAGGAAAG AAAAAAGATG GTCAAGGAAG CCCAGAGAGA GAAGAGAAAA 
AATAAAATCC CTAAACATGT GAAAAAAAGA AAGGAAAAAA CAGCCAAGGC CAAAAAAGGC 
AAATAG