RIOK1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RIOK1
Entrez Gene ID	83732
Full Name	RIO kinase 1
Synonyms	AD034,RIO1,RRP10,bA288G3.1
General protein information	Preferred Names RIO kinase 1 Names serine/threonine-protein kinase RIO1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p24.3
Summary	The protein encoded by this gene competes with pICln for inclusion in the protein arginine methyltransferase 5 complex. This complex targets substrates for dimethylation. The encoded protein is essential for the last steps in the maturation of 40S subunits. [provided by RefSeq, Jan 2017].
Disorder MIM:	617753

mRNA and Protein(s)

mRNA	Protein	Name
XM_011514933.3	XP_011513235.2	serine/threonine-protein kinase RIO1 isoform X1
NM_031480.3	NP_113668.2	serine/threonine-protein kinase RIO1 isoform 1
NM_001348194.1	NP_001335123.1	serine/threonine-protein kinase RIO1 isoform 3
NM_031480.2	NP_113668.2	serine/threonine-protein kinase RIO1 isoform 1
XM_011514933.2	XP_011513235.2	serine/threonine-protein kinase RIO1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RIOK1	XP_527225.2
Drosophila melanogaster (fruit fly)	CG11660	NP_648489.1
Kluyveromyces lactis	KLLA0E21121g	XP_454905.1
Gallus gallus (chicken)	RIOK1	XP_418958.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012121	XP_320408.4
Saccharomyces cerevisiae (baker's yeast)	RIO1	NP_014762.3
Eremothecium gossypii	AGOS_AER257W	NP_985114.1
Arabidopsis thaliana (thale cress)	AT5G37350	NP_851100.1
Schizosaccharomyces pombe (fission yeast)	SPAC10F6.10	NP_593261.2
Magnaporthe oryzae (rice blast fungus)	MGG_13447	XP_003711604.1
Xenopus tropicalis (tropical clawed frog)	riok1	XP_002932721.2
Homo sapiens (human)	RIOK1	NP_113668.2
Canis lupus familiaris (dog)	RIOK1	XP_535878.1
Bos taurus (cattle)	RIOK1	XP_002697645.1
Mus musculus (house mouse)	Riok1	NP_077204.2
Caenorhabditis elegans (roundworm)	riok-1	NP_001021570.2
Neurospora crassa	NCU10652	XP_956116.2
Arabidopsis thaliana (thale cress)	AT2G24990	NP_180071.1
	RIOK1	XP_001084661.1
Rattus norvegicus (Norway rat)	Riok1	NP_001093981.1
Danio rerio (zebrafish)	riok1	NP_998160.1
Oryza sativa (rice)	Os07g0162300	NP_001058946.1

Gene Ontology
Bibliography

Related articles in PubMed

The Atypical Kinase RIOK1 Promotes Tumor Growth and Invasive Behavior.
Weinberg F, Reischmann N, Fauth L, Taromi S, Mastroianni J, K?hler M, Halbach S, Becker AC, Deng N, Schmitz T, Uhl FM, Herbener N, Riedel B, Beier F, Swarbrick A, Lassmann S, Dengjel J, Zeiser R, Brummer T
EBioMedicine2079-97(2017 Jun)

Benzimidazole inhibitors of protein kinase CK2 potently inhibit the activity of atypical protein kinase Rio1.
Kubi?ski K, Mas?yk M, Orzeszko A
Molecular and cellular biochemistry426(1-2)195-203(2017 Feb)

MTAP Deletions in Cancer Create Vulnerability to Targeting of the MAT2A/PRMT5/RIOK1 Axis.
Marjon K, Cameron MJ, Quang P, Clasquin MF, Mandley E, Kunii K, McVay M, Choe S, Kernytsky A, Gross S, Konteatis Z, Murtie J, Blake ML, Travins J, Dorsch M, Biller SA, Marks KM
Cell reports15(3)574-587(2016 Apr)

Dominant Rio1 kinase/ATPase catalytic mutant induces trapping of late pre-40S biogenesis factors in 80S-like ribosomes.
Ferreira-Cerca S, Kiburu I, Thomson E, LaRonde N, Hurt E
Nucleic acids research42(13)8635-47(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Docking studies revealed that TIBI can occupy the ATP-binding site of Rio1 in a manner similar to toyocamycin, and enhances the thermostability of the enzyme.
Title: Benzimidazole inhibitors of protein kinase CK2 potently inhibit the activity of atypical protein kinase Rio1.

methionine adenosyltransferase II alpha (MAT2A), and the arginine methyltransferase, PRMT5, as vulnerable enzymes in cells with MTAP deletion.
Title: MTAP Deletions in Cancer Create Vulnerability to Targeting of the MAT2A/PRMT5/RIOK1 Axis.

The RIO kinase fold generates a versatile ATPase enzyme, which in the case of Rio1 is activated following the Rio2 step to regulate one of the final 40S maturation events, at which time the 60S subunit is recruited for final quality control check.
Title: Dominant Rio1 kinase/ATPase catalytic mutant induces trapping of late pre-40S biogenesis factors in 80S-like ribosomes.

These results imply that, in glioblastoma cells, constitutive Akt signaling drives RIO kinase overexpression, which creates a feedforward loop that promotes and maintains oncogenic Akt activity through stimulation of mTor signaling.
Title: A kinome-wide RNAi screen in Drosophila Glia reveals that the RIO kinases mediate cell proliferation and survival through TORC2-Akt signaling in glioblastoma.

These data reveal a role of hRio1 in the final stages of cytoplasmic pre-40S maturation
Title: The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits.

The following RIOK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIOK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31293	NM_031480.3 Latest version!	Homo sapiens RIO kinase 1 (RIOK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu40499	NM_001348194.1 Latest version!	Homo sapiens RIO kinase 1 (RIOK1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu96827	XM_011514933.3 Latest version!	Homo sapiens RIO kinase 1 (RIOK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu31293	NM_031480.2	Homo sapiens RIO kinase 1 (RIOK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu96827	XM_011514933.2	Homo sapiens RIO kinase 1 (RIOK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RIOK1 ( NM_031480.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_113668.2 Homo sapiens RIO kinase 1 (RIOK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31293
Clone ID Related Accession (Same CDS sequence)	NM_031480.3 , NM_031480.2
Accession Version	NM_031480.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase RIO1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139095.15 and AL138878.10. On Nov 25, 2018 this sequence version replaced NM_031480.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1097208.1, SRR1803617.170551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000379834.7/ ENSP00000369162.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACTACC GGCGGCTTCT CATGAGCCGG GTGGTCCCCG GGCAATTCGA CGACGCGGAC 
TCCTCTGACA GTGAAAACAG AGACTTGAAG ACAGTCAAAG AGAAGGATGA CATTCTGTTT 
GAAGACCTTC AAGACAATGT GAATGAGAAT GGTGAAGGTG AAATAGAAGA TGAGGAGGAG 
GAGGGTTATG ACGATGATGA TGATGACTGG GACTGGGATG AAGGAGTTGG AAAACTCGCC 
AAGGGTTATG TCTGGAATGG AGGAAGCAAC CCACAGGCAA ATCGACAGAC CTCCGACAGC 
AGTTCAGCCA AAATGTCTAC TCCAGCAGAC AAGGTCTTAC GGAAATTTGA GAATAAAATT 
AATTTAGATA AGCTAAATGT TACTGATTCC GTCATAAATA AAGTCACCGA AAAGTCTAGA 
CAAAAGGAAG CAGATATGTA TCGCATCAAA GATAAGGCAG ACAGAGCAAC TGTAGAACAG 
GTGTTGGATC CCAGAACAAG AATGATTTTA TTCAAGATGT TGACTAGAGG AATCATAACA 
GAGATAAATG GCTGCATTAG CACAGGAAAA GAAGCTAATG TATACCATGC TAGCACAGCA 
AATGGAGAGA GCAGAGCAAT CAAAATTTAT AAAACTTCTA TTTTGGTGTT CAAAGATCGG 
GATAAATATG TAAGTGGAGA ATTCAGATTT CGTCATGGCT ATTGTAAAGG AAACCCTAGG 
AAAATGGTGA AAACTTGGGC AGAAAAAGAA ATGAGGAACT TAATCAGGCT AAACACAGCA 
GAGATACCAT GTCCAGAACC AATAATGCTA AGAAGTCATG TTCTTGTCAT GAGTTTCATC 
GGTAAAGATG ACATGCCTGC ACCACTCTTG AAAAATGTCC AGTTATCAGA ATCCAAGGCT 
CGGGAGTTGT ACCTGCAGGT CATTCAGTAC ATGAGAAGAA TGTATCAGGA TGCCAGACTT 
GTCCATGCAG ATCTCAGTGA ATTTAACATG CTGTACCACG GTGGAGGCGT GTATATCATT 
GACGTGTCTC AGTCCGTGGA GCACGACCAC CCACATGCCT TGGAGTTCTT GAGAAAGGAT 
TGCGCCAACG TCAATGATTT CTTTATGAGG CACAGTGTTG CTGTCATGAC TGTGCGGGAG 
CTCTTTGAAT TTGTCACAGA TCCATCCATT ACACATGAGA ACATGGATGC TTATCTCTCA 
AAGGCCATGG AAATAGCATC TCAAAGGACC AAGGAAGAAC GGTCTAGCCA AGATCATGTG 
GATGAAGAGG TGTTTAAGCG AGCATATATT CCTAGAACCT TGAATGAAGT GAAAAATTAT 
GAGAGGGATA TGGACATAAT TATGAAATTG AAGGAAGAGG ACATGGCCAT GAATGCCCAA 
CAAGATAATA TTCTATACCA GACTGTTACA GGATTGAAGA AAGATTTGTC AGGAGTTCAG 
AAGGTCCCTG CACTCCTAGA AAATCAAGTG GAGGAAAGGA CTTGTTCTGA TTCAGAAGAT 
ATTGGAAGCT CTGAGTGCTC TGACACAGAC TCTGAAGAGC AGGGAGACCA TGCCCGCCCC 
AAGAAACACA CCACGGACCC TGACATTGAT AAAAAAGAAA GAAAAAAGAT GGTCAAGGAA 
GCCCAGAGAG AGAAAAGAAA AAACAAAATT CCTAAACATG TGAAAAAAAG AAAGGAGAAG 
ACAGCCAAGA CGAAAAAAGG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113668.2
CDS	196..1902
Translation	MDYRRLLMSRVVPGQFDDADSSDSENRDLKTVKEKDDILFEDLQDNVNENGEGEIEDEEEEGYDDDDDDWDWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSRQKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHASTANGESRAIKIYKTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK

Target ORF information:

RefSeq Version	NM_031480.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RIO kinase 1 (RIOK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031480.3

ORF Insert Sequence:

ATGGACTACC GGCGGCTTCT CATGAGCCGG GTGGTCCCCG GGCAATTCGA CGACGCGGAC 
TCCTCTGACA GTGAAAACAG AGACTTGAAG ACAGTCAAAG AGAAGGATGA CATTCTGTTT 
GAAGACCTTC AAGACAATGT GAATGAGAAT GGTGAAGGTG AAATAGAAGA TGAGGAGGAG 
GAGGGTTATG ACGATGATGA TGATGACTGG GACTGGGATG AAGGAGTTGG AAAACTCGCC 
AAGGGTTATG TCTGGAATGG AGGAAGCAAC CCACAGGCAA ATCGACAGAC CTCCGACAGC 
AGTTCAGCCA AAATGTCTAC TCCAGCAGAC AAGGTCTTAC GGAAATTTGA GAATAAAATT 
AATTTAGATA AGCTAAATGT TACTGATTCC GTCATAAATA AAGTCACCGA AAAGTCTAGA 
CAAAAGGAAG CAGATATGTA TCGCATCAAA GATAAGGCAG ACAGAGCAAC TGTAGAACAG 
GTGTTGGATC CCAGAACAAG AATGATTTTA TTCAAGATGT TGACTAGAGG AATCATAACA 
GAGATAAATG GCTGCATTAG CACAGGAAAA GAAGCTAATG TATACCATGC TAGCACAGCA 
AATGGAGAGA GCAGAGCAAT CAAAATTTAT AAAACTTCTA TTTTGGTGTT CAAAGATCGG 
GATAAATATG TAAGTGGAGA ATTCAGATTT CGTCATGGCT ATTGTAAAGG AAACCCTAGG 
AAAATGGTGA AAACTTGGGC AGAAAAAGAA ATGAGGAACT TAATCAGGCT AAACACAGCA 
GAGATACCAT GTCCAGAACC AATAATGCTA AGAAGTCATG TTCTTGTCAT GAGTTTCATC 
GGTAAAGATG ACATGCCTGC ACCACTCTTG AAAAATGTCC AGTTATCAGA ATCCAAGGCT 
CGGGAGTTGT ACCTGCAGGT CATTCAGTAC ATGAGAAGAA TGTATCAGGA TGCCAGACTT 
GTCCATGCAG ATCTCAGTGA ATTTAACATG CTGTACCACG GTGGAGGCGT GTATATCATT 
GACGTGTCTC AGTCCGTGGA GCACGACCAC CCACATGCCT TGGAGTTCTT GAGAAAGGAT 
TGCGCCAACG TCAATGATTT CTTTATGAGG CACAGTGTTG CTGTCATGAC TGTGCGGGAG 
CTCTTTGAAT TTGTCACAGA TCCATCCATT ACACATGAGA ACATGGATGC TTATCTCTCA 
AAGGCCATGG AAATAGCATC TCAAAGGACC AAGGAAGAAC GGTCTAGCCA AGATCATGTG 
GATGAAGAGG TGTTTAAGCG AGCATATATT CCTAGAACCT TGAATGAAGT GAAAAATTAT 
GAGAGGGATA TGGACATAAT TATGAAATTG AAGGAAGAGG ACATGGCCAT GAATGCCCAA 
CAAGATAATA TTCTATACCA GACTGTTACA GGATTGAAGA AAGATTTGTC AGGAGTTCAG 
AAGGTCCCTG CACTCCTAGA AAATCAAGTG GAGGAAAGGA CTTGTTCTGA TTCAGAAGAT 
ATTGGAAGCT CTGAGTGCTC TGACACAGAC TCTGAAGAGC AGGGAGACCA TGCCCGCCCC 
AAGAAACACA CCACGGACCC TGACATTGAT AAAAAAGAAA GAAAAAAGAT GGTCAAGGAA 
GCCCAGAGAG AGAAAAGAAA AAACAAAATT CCTAAACATG TGAAAAAAAG AAAGGAGAAG 
ACAGCCAAGA CGAAAAAAGG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIOK1 ( NM_031480.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_113668.2 Homo sapiens RIO kinase 1 (RIOK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31293
Clone ID Related Accession (Same CDS sequence)	NM_031480.3 , NM_031480.2
Accession Version	NM_031480.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase RIO1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC006104.1. On Oct 7, 2002 this sequence version replaced NM_031480.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC006104.1, SRR1803617.170551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTACC GGCGGCTTCT CATGAGCCGG GTGGTCCCCG GGCAATTCGA CGACGCGGAC 
TCCTCTGACA GTGAAAACAG AGACTTGAAG ACAGTCAAAG AGAAGGATGA CATTCTGTTT 
GAAGACCTTC AAGACAATGT GAATGAGAAT GGTGAAGGTG AAATAGAAGA TGAGGAGGAG 
GAGGGTTATG ACGATGATGA TGATGACTGG GACTGGGATG AAGGAGTTGG AAAACTCGCC 
AAGGGTTATG TCTGGAATGG AGGAAGCAAC CCACAGGCAA ATCGACAGAC CTCCGACAGC 
AGTTCAGCCA AAATGTCTAC TCCAGCAGAC AAGGTCTTAC GGAAATTTGA GAATAAAATT 
AATTTAGATA AGCTAAATGT TACTGATTCC GTCATAAATA AAGTCACCGA AAAGTCTAGA 
CAAAAGGAAG CAGATATGTA TCGCATCAAA GATAAGGCAG ACAGAGCAAC TGTAGAACAG 
GTGTTGGATC CCAGAACAAG AATGATTTTA TTCAAGATGT TGACTAGAGG AATCATAACA 
GAGATAAATG GCTGCATTAG CACAGGAAAA GAAGCTAATG TATACCATGC TAGCACAGCA 
AATGGAGAGA GCAGAGCAAT CAAAATTTAT AAAACTTCTA TTTTGGTGTT CAAAGATCGG 
GATAAATATG TAAGTGGAGA ATTCAGATTT CGTCATGGCT ATTGTAAAGG AAACCCTAGG 
AAAATGGTGA AAACTTGGGC AGAAAAAGAA ATGAGGAACT TAATCAGGCT AAACACAGCA 
GAGATACCAT GTCCAGAACC AATAATGCTA AGAAGTCATG TTCTTGTCAT GAGTTTCATC 
GGTAAAGATG ACATGCCTGC ACCACTCTTG AAAAATGTCC AGTTATCAGA ATCCAAGGCT 
CGGGAGTTGT ACCTGCAGGT CATTCAGTAC ATGAGAAGAA TGTATCAGGA TGCCAGACTT 
GTCCATGCAG ATCTCAGTGA ATTTAACATG CTGTACCACG GTGGAGGCGT GTATATCATT 
GACGTGTCTC AGTCCGTGGA GCACGACCAC CCACATGCCT TGGAGTTCTT GAGAAAGGAT 
TGCGCCAACG TCAATGATTT CTTTATGAGG CACAGTGTTG CTGTCATGAC TGTGCGGGAG 
CTCTTTGAAT TTGTCACAGA TCCATCCATT ACACATGAGA ACATGGATGC TTATCTCTCA 
AAGGCCATGG AAATAGCATC TCAAAGGACC AAGGAAGAAC GGTCTAGCCA AGATCATGTG 
GATGAAGAGG TGTTTAAGCG AGCATATATT CCTAGAACCT TGAATGAAGT GAAAAATTAT 
GAGAGGGATA TGGACATAAT TATGAAATTG AAGGAAGAGG ACATGGCCAT GAATGCCCAA 
CAAGATAATA TTCTATACCA GACTGTTACA GGATTGAAGA AAGATTTGTC AGGAGTTCAG 
AAGGTCCCTG CACTCCTAGA AAATCAAGTG GAGGAAAGGA CTTGTTCTGA TTCAGAAGAT 
ATTGGAAGCT CTGAGTGCTC TGACACAGAC TCTGAAGAGC AGGGAGACCA TGCCCGCCCC 
AAGAAACACA CCACGGACCC TGACATTGAT AAAAAAGAAA GAAAAAAGAT GGTCAAGGAA 
GCCCAGAGAG AGAAAAGAAA AAACAAAATT CCTAAACATG TGAAAAAAAG AAAGGAGAAG 
ACAGCCAAGA CGAAAAAAGG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113668.2
CDS	175..1881
Translation	MDYRRLLMSRVVPGQFDDADSSDSENRDLKTVKEKDDILFEDLQDNVNENGEGEIEDEEEEGYDDDDDDWDWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSRQKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHASTANGESRAIKIYKTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK

Target ORF information:

RefSeq Version	NM_031480.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RIO kinase 1 (RIOK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031480.2

ORF Insert Sequence:

ATGGACTACC GGCGGCTTCT CATGAGCCGG GTGGTCCCCG GGCAATTCGA CGACGCGGAC 
TCCTCTGACA GTGAAAACAG AGACTTGAAG ACAGTCAAAG AGAAGGATGA CATTCTGTTT 
GAAGACCTTC AAGACAATGT GAATGAGAAT GGTGAAGGTG AAATAGAAGA TGAGGAGGAG 
GAGGGTTATG ACGATGATGA TGATGACTGG GACTGGGATG AAGGAGTTGG AAAACTCGCC 
AAGGGTTATG TCTGGAATGG AGGAAGCAAC CCACAGGCAA ATCGACAGAC CTCCGACAGC 
AGTTCAGCCA AAATGTCTAC TCCAGCAGAC AAGGTCTTAC GGAAATTTGA GAATAAAATT 
AATTTAGATA AGCTAAATGT TACTGATTCC GTCATAAATA AAGTCACCGA AAAGTCTAGA 
CAAAAGGAAG CAGATATGTA TCGCATCAAA GATAAGGCAG ACAGAGCAAC TGTAGAACAG 
GTGTTGGATC CCAGAACAAG AATGATTTTA TTCAAGATGT TGACTAGAGG AATCATAACA 
GAGATAAATG GCTGCATTAG CACAGGAAAA GAAGCTAATG TATACCATGC TAGCACAGCA 
AATGGAGAGA GCAGAGCAAT CAAAATTTAT AAAACTTCTA TTTTGGTGTT CAAAGATCGG 
GATAAATATG TAAGTGGAGA ATTCAGATTT CGTCATGGCT ATTGTAAAGG AAACCCTAGG 
AAAATGGTGA AAACTTGGGC AGAAAAAGAA ATGAGGAACT TAATCAGGCT AAACACAGCA 
GAGATACCAT GTCCAGAACC AATAATGCTA AGAAGTCATG TTCTTGTCAT GAGTTTCATC 
GGTAAAGATG ACATGCCTGC ACCACTCTTG AAAAATGTCC AGTTATCAGA ATCCAAGGCT 
CGGGAGTTGT ACCTGCAGGT CATTCAGTAC ATGAGAAGAA TGTATCAGGA TGCCAGACTT 
GTCCATGCAG ATCTCAGTGA ATTTAACATG CTGTACCACG GTGGAGGCGT GTATATCATT 
GACGTGTCTC AGTCCGTGGA GCACGACCAC CCACATGCCT TGGAGTTCTT GAGAAAGGAT 
TGCGCCAACG TCAATGATTT CTTTATGAGG CACAGTGTTG CTGTCATGAC TGTGCGGGAG 
CTCTTTGAAT TTGTCACAGA TCCATCCATT ACACATGAGA ACATGGATGC TTATCTCTCA 
AAGGCCATGG AAATAGCATC TCAAAGGACC AAGGAAGAAC GGTCTAGCCA AGATCATGTG 
GATGAAGAGG TGTTTAAGCG AGCATATATT CCTAGAACCT TGAATGAAGT GAAAAATTAT 
GAGAGGGATA TGGACATAAT TATGAAATTG AAGGAAGAGG ACATGGCCAT GAATGCCCAA 
CAAGATAATA TTCTATACCA GACTGTTACA GGATTGAAGA AAGATTTGTC AGGAGTTCAG 
AAGGTCCCTG CACTCCTAGA AAATCAAGTG GAGGAAAGGA CTTGTTCTGA TTCAGAAGAT 
ATTGGAAGCT CTGAGTGCTC TGACACAGAC TCTGAAGAGC AGGGAGACCA TGCCCGCCCC 
AAGAAACACA CCACGGACCC TGACATTGAT AAAAAAGAAA GAAAAAAGAT GGTCAAGGAA 
GCCCAGAGAG AGAAAAGAAA AAACAAAATT CCTAAACATG TGAAAAAAAG AAAGGAGAAG 
ACAGCCAAGA CGAAAAAAGG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIOK1 ( NM_001348194.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335123.1 Homo sapiens RIO kinase 1 (RIOK1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu40499
Clone ID Related Accession (Same CDS sequence)	NM_001348194.1
Accession Version	NM_001348194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase RIO1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139095.15 and AL138878.10. On Jan 14, 2017 this sequence version replaced XM_005249446.3. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.150073.1, SRR1803616.198636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTACTC CAGCAGACAA GGTCTTACGG AAATTTGAGA ATAAAATTAA TTTAGATAAG 
CTAAATGTTA CTGATTCCGT CATAAATAAA GTCACCGAAA AGTCTAGACA AAAGGAAGCA 
GATATGTATC GCATCAAAGA TAAGGCAGAC AGAGCAACTG TAGAACAGGT GTTGGATCCC 
AGAACAAGAA TGATTTTATT CAAGATGTTG ACTAGAGGAA TCATAACAGA GATAAATGGC 
TGCATTAGCA CAGGAAAAGA AGCTAATGTA TACCATGCTA GCACAGCAAA TGGAGAGAGC 
AGAGCAATCA AAATTTATAA AACTTCTATT TTGGTGTTCA AAGATCGGGA TAAATATGTA 
AGTGGAGAAT TCAGATTTCG TCATGGCTAT TGTAAAGGAA ACCCTAGGAA AATGGTGAAA 
ACTTGGGCAG AAAAAGAAAT GAGGAACTTA ATCAGGCTAA ACACAGCAGA GATACCATGT 
CCAGAACCAA TAATGCTAAG AAGTCATGTT CTTGTCATGA GTTTCATCGG TAAAGATGAC 
ATGCCTGCAC CACTCTTGAA AAATGTCCAG TTATCAGAAT CCAAGGCTCG GGAGTTGTAC 
CTGCAGGTCA TTCAGTACAT GAGAAGAATG TATCAGGATG CCAGACTTGT CCATGCAGAT 
CTCAGTGAAT TTAACATGCT GTACCACGGT GGAGGCGTGT ATATCATTGA CGTGTCTCAG 
TCCGTGGAGC ACGACCACCC ACATGCCTTG GAGTTCTTGA GAAAGGATTG CGCCAACGTC 
AATGATTTCT TTATGAGGCA CAGTGTTGCT GTCATGACTG TGCGGGAGCT CTTTGAATTT 
GTCACAGATC CATCCATTAC ACATGAGAAC ATGGATGCTT ATCTCTCAAA GGCCATGGAA 
ATAGCATCTC AAAGGACCAA GGAAGAACGG TCTAGCCAAG ATCATGTGGA TGAAGAGGTG 
TTTAAGCGAG CATATATTCC TAGAACCTTG AATGAAGTGA AAAATTATGA GAGGGATATG 
GACATAATTA TGAAATTGAA GGAAGAGGAC ATGGCCATGA ATGCCCAACA AGATAATATT 
CTATACCAGA CTGTTACAGG ATTGAAGAAA GATTTGTCAG GAGTTCAGAA GGTCCCTGCA 
CTCCTAGAAA ATCAAGTGGA GGAAAGGACT TGTTCTGATT CAGAAGATAT TGGAAGCTCT 
GAGTGCTCTG ACACAGACTC TGAAGAGCAG GGAGACCATG CCCGCCCCAA GAAACACACC 
ACGGACCCTG ACATTGATAA AAAAGAAAGA AAAAAGATGG TCAAGGAAGC CCAGAGAGAG 
AAAAGAAAAA ACAAAATTCC TAAACATGTG AAAAAAAGAA AGGAGAAGAC AGCCAAGACG 
AAAAAAGGCA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335123.1
CDS	519..1913
Translation	MSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSRQKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHASTANGESRAIKIYKTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK

Target ORF information:

RefSeq Version	NM_001348194.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RIO kinase 1 (RIOK1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348194.1

ORF Insert Sequence:

ATGTCTACTC CAGCAGACAA GGTCTTACGG AAATTTGAGA ATAAAATTAA TTTAGATAAG 
CTAAATGTTA CTGATTCCGT CATAAATAAA GTCACCGAAA AGTCTAGACA AAAGGAAGCA 
GATATGTATC GCATCAAAGA TAAGGCAGAC AGAGCAACTG TAGAACAGGT GTTGGATCCC 
AGAACAAGAA TGATTTTATT CAAGATGTTG ACTAGAGGAA TCATAACAGA GATAAATGGC 
TGCATTAGCA CAGGAAAAGA AGCTAATGTA TACCATGCTA GCACAGCAAA TGGAGAGAGC 
AGAGCAATCA AAATTTATAA AACTTCTATT TTGGTGTTCA AAGATCGGGA TAAATATGTA 
AGTGGAGAAT TCAGATTTCG TCATGGCTAT TGTAAAGGAA ACCCTAGGAA AATGGTGAAA 
ACTTGGGCAG AAAAAGAAAT GAGGAACTTA ATCAGGCTAA ACACAGCAGA GATACCATGT 
CCAGAACCAA TAATGCTAAG AAGTCATGTT CTTGTCATGA GTTTCATCGG TAAAGATGAC 
ATGCCTGCAC CACTCTTGAA AAATGTCCAG TTATCAGAAT CCAAGGCTCG GGAGTTGTAC 
CTGCAGGTCA TTCAGTACAT GAGAAGAATG TATCAGGATG CCAGACTTGT CCATGCAGAT 
CTCAGTGAAT TTAACATGCT GTACCACGGT GGAGGCGTGT ATATCATTGA CGTGTCTCAG 
TCCGTGGAGC ACGACCACCC ACATGCCTTG GAGTTCTTGA GAAAGGATTG CGCCAACGTC 
AATGATTTCT TTATGAGGCA CAGTGTTGCT GTCATGACTG TGCGGGAGCT CTTTGAATTT 
GTCACAGATC CATCCATTAC ACATGAGAAC ATGGATGCTT ATCTCTCAAA GGCCATGGAA 
ATAGCATCTC AAAGGACCAA GGAAGAACGG TCTAGCCAAG ATCATGTGGA TGAAGAGGTG 
TTTAAGCGAG CATATATTCC TAGAACCTTG AATGAAGTGA AAAATTATGA GAGGGATATG 
GACATAATTA TGAAATTGAA GGAAGAGGAC ATGGCCATGA ATGCCCAACA AGATAATATT 
CTATACCAGA CTGTTACAGG ATTGAAGAAA GATTTGTCAG GAGTTCAGAA GGTCCCTGCA 
CTCCTAGAAA ATCAAGTGGA GGAAAGGACT TGTTCTGATT CAGAAGATAT TGGAAGCTCT 
GAGTGCTCTG ACACAGACTC TGAAGAGCAG GGAGACCATG CCCGCCCCAA GAAACACACC 
ACGGACCCTG ACATTGATAA AAAAGAAAGA AAAAAGATGG TCAAGGAAGC CCAGAGAGAG 
AAAAGAAAAA ACAAAATTCC TAAACATGTG AAAAAAAGAA AGGAGAAGAC AGCCAAGACG 
AAAAAAGGCA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIOK1 ( XM_011514933.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513235.2 Homo sapiens RIO kinase 1 (RIOK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu96827
Clone ID Related Accession (Same CDS sequence)	XM_011514933.3 , XM_011514933.2
Accession Version	XM_011514933.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase RIO1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514933.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTCT TGAATAGTAG GCTTTGGGCA TGGAGAATGA GCAAACAACT GCAACAGAAC 
TGGAAAACAG GAGAACATGG TTTTAAGGAG ATGGAGCTTA TCGGAAGTGA AAACAGAGAC 
TTGAAGACAG TCAAAGAGAA GGATGACATT CTGTTTGAAG ACCTTCAAGA CAATGTGAAT 
GAGAATGGTG AAGGTGAAAT AGAAGATGAG GAGGAGGAGG GTTATGACGA TGATGATGAT 
GACTGGGACT GGGATGAAGG AGTTGGAAAA CTCGCCAAGG GTTATGTCTG GAATGGAGGA 
AGCAACCCAC AGGCAAATCG ACAGACCTCC GACAGCAGTT CAGCCAAAAT GTCTACTCCA 
GCAGACAAGG TCTTACGGAA ATTTGAGAAT AAAATTAATT TAGATAAGCT AAATGTTACT 
GATTCCGTCA TAAATAAAGT CACCGAAAAG TCTAGACAAA AGGAAGCAGA TATGTATCGC 
ATCAAAGATA AGGCAGACAG AGCAACTGTA GAACAGGTGT TGGATCCCAG AACAAGAATG 
ATTTTATTCA AGATGTTGAC TAGAGGAATC ATAACAGAGA TAAATGGCTG CATTAGCACA 
GGAAAAGAAG CTAATGTATA CCATGCTAGC ACAGCAAATG GAGAGAGCAG AGCAATCAAA 
ATTTATAAAA CTTCTATTTT GGTGTTCAAA GATCGGGATA AATATGTAAG TGGAGAATTC 
AGATTTCGTC ATGGCTATTG TAAAGGAAAC CCTAGGAAAA TGGTGAAAAC TTGGGCAGAA 
AAAGAAATGA GGAACTTAAT CAGGCTAAAC ACAGCAGAGA TACCATGTCC AGAACCAATA 
ATGCTAAGAA GTCATGTTCT TGTCATGAGT TTCATCGGTA AAGATGACAT GCCTGCACCA 
CTCTTGAAAA ATGTCCAGTT ATCAGAATCC AAGGCTCGGG AGTTGTACCT GCAGGTCATT 
CAGTACATGA GAAGAATGTA TCAGGATGCC AGACTTGTCC ATGCAGATCT CAGTGAATTT 
AACATGCTGT ACCACGGTGG AGGCGTGTAT ATCATTGACG TGTCTCAGTC CGTGGAGCAC 
GACCACCCAC ATGCCTTGGA GTTCTTGAGA AAGGATTGCG CCAACGTCAA TGATTTCTTT 
ATGAGGCACA GTGTTGCTGT CATGACTGTG CGGGAGCTCT TTGAATTTGT CACAGATCCA 
TCCATTACAC ATGAGAACAT GGATGCTTAT CTCTCAAAGG CCATGGAAAT AGCATCTCAA 
AGGACCAAGG AAGAACGGTC TAGCCAAGAT CATGTGGATG AAGAGGTGTT TAAGCGAGCA 
TATATTCCTA GAACCTTGAA TGAAGTGAAA AATTATGAGA GGGATATGGA CATAATTATG 
AAATTGAAGG AAGAGGACAT GGCCATGAAT GCCCAACAAG ATAATATTCT ATACCAGACT 
GTTACAGGAT TGAAGAAAGA TTTGTCAGGA GTTCAGAAGG TCCCTGCACT CCTAGAAAAT 
CAAGTGGAGG AAAGGACTTG TTCTGATTCA GAAGATATTG GAAGCTCTGA GTGCTCTGAC 
ACAGACTCTG AAGAGCAGGG AGACCATGCC CGCCCCAAGA AACACACCAC GGACCCTGAC 
ATTGATAAAA AAGAAAGAAA AAAGATGGTC AAGGAAGCCC AGAGAGAGAA AAGAAAAAAC 
AAAATTCCTA AACATGTGAA AAAAAGAAAG GAGAAGACAG CCAAGACGAA AAAAGGCAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513235.2
CDS	95..1837
Translation	MCFLNSRLWAWRMSKQLQQNWKTGEHGFKEMELIGSENRDLKTVKEKDDILFEDLQDNVNENGEGEIEDEEEEGYDDDDDDWDWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSRQKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHASTANGESRAIKIYKTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK

Target ORF information:

RefSeq Version	XM_011514933.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RIO kinase 1 (RIOK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514933.3

ORF Insert Sequence:

ATGTGCTTCT TGAATAGTAG GCTTTGGGCA TGGAGAATGA GCAAACAACT GCAACAGAAC 
TGGAAAACAG GAGAACATGG TTTTAAGGAG ATGGAGCTTA TCGGAAGTGA AAACAGAGAC 
TTGAAGACAG TCAAAGAGAA GGATGACATT CTGTTTGAAG ACCTTCAAGA CAATGTGAAT 
GAGAATGGTG AAGGTGAAAT AGAAGATGAG GAGGAGGAGG GTTATGACGA TGATGATGAT 
GACTGGGACT GGGATGAAGG AGTTGGAAAA CTCGCCAAGG GTTATGTCTG GAATGGAGGA 
AGCAACCCAC AGGCAAATCG ACAGACCTCC GACAGCAGTT CAGCCAAAAT GTCTACTCCA 
GCAGACAAGG TCTTACGGAA ATTTGAGAAT AAAATTAATT TAGATAAGCT AAATGTTACT 
GATTCCGTCA TAAATAAAGT CACCGAAAAG TCTAGACAAA AGGAAGCAGA TATGTATCGC 
ATCAAAGATA AGGCAGACAG AGCAACTGTA GAACAGGTGT TGGATCCCAG AACAAGAATG 
ATTTTATTCA AGATGTTGAC TAGAGGAATC ATAACAGAGA TAAATGGCTG CATTAGCACA 
GGAAAAGAAG CTAATGTATA CCATGCTAGC ACAGCAAATG GAGAGAGCAG AGCAATCAAA 
ATTTATAAAA CTTCTATTTT GGTGTTCAAA GATCGGGATA AATATGTAAG TGGAGAATTC 
AGATTTCGTC ATGGCTATTG TAAAGGAAAC CCTAGGAAAA TGGTGAAAAC TTGGGCAGAA 
AAAGAAATGA GGAACTTAAT CAGGCTAAAC ACAGCAGAGA TACCATGTCC AGAACCAATA 
ATGCTAAGAA GTCATGTTCT TGTCATGAGT TTCATCGGTA AAGATGACAT GCCTGCACCA 
CTCTTGAAAA ATGTCCAGTT ATCAGAATCC AAGGCTCGGG AGTTGTACCT GCAGGTCATT 
CAGTACATGA GAAGAATGTA TCAGGATGCC AGACTTGTCC ATGCAGATCT CAGTGAATTT 
AACATGCTGT ACCACGGTGG AGGCGTGTAT ATCATTGACG TGTCTCAGTC CGTGGAGCAC 
GACCACCCAC ATGCCTTGGA GTTCTTGAGA AAGGATTGCG CCAACGTCAA TGATTTCTTT 
ATGAGGCACA GTGTTGCTGT CATGACTGTG CGGGAGCTCT TTGAATTTGT CACAGATCCA 
TCCATTACAC ATGAGAACAT GGATGCTTAT CTCTCAAAGG CCATGGAAAT AGCATCTCAA 
AGGACCAAGG AAGAACGGTC TAGCCAAGAT CATGTGGATG AAGAGGTGTT TAAGCGAGCA 
TATATTCCTA GAACCTTGAA TGAAGTGAAA AATTATGAGA GGGATATGGA CATAATTATG 
AAATTGAAGG AAGAGGACAT GGCCATGAAT GCCCAACAAG ATAATATTCT ATACCAGACT 
GTTACAGGAT TGAAGAAAGA TTTGTCAGGA GTTCAGAAGG TCCCTGCACT CCTAGAAAAT 
CAAGTGGAGG AAAGGACTTG TTCTGATTCA GAAGATATTG GAAGCTCTGA GTGCTCTGAC 
ACAGACTCTG AAGAGCAGGG AGACCATGCC CGCCCCAAGA AACACACCAC GGACCCTGAC 
ATTGATAAAA AAGAAAGAAA AAAGATGGTC AAGGAAGCCC AGAGAGAGAA AAGAAAAAAC 
AAAATTCCTA AACATGTGAA AAAAAGAAAG GAGAAGACAG CCAAGACGAA AAAAGGCAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIOK1 ( XM_011514933.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513235.2 Homo sapiens RIO kinase 1 (RIOK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu96827
Clone ID Related Accession (Same CDS sequence)	XM_011514933.3 , XM_011514933.2
Accession Version	XM_011514933.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase RIO1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTCT TGAATAGTAG GCTTTGGGCA TGGAGAATGA GCAAACAACT GCAACAGAAC 
TGGAAAACAG GAGAACATGG TTTTAAGGAG ATGGAGCTTA TCGGAAGTGA AAACAGAGAC 
TTGAAGACAG TCAAAGAGAA GGATGACATT CTGTTTGAAG ACCTTCAAGA CAATGTGAAT 
GAGAATGGTG AAGGTGAAAT AGAAGATGAG GAGGAGGAGG GTTATGACGA TGATGATGAT 
GACTGGGACT GGGATGAAGG AGTTGGAAAA CTCGCCAAGG GTTATGTCTG GAATGGAGGA 
AGCAACCCAC AGGCAAATCG ACAGACCTCC GACAGCAGTT CAGCCAAAAT GTCTACTCCA 
GCAGACAAGG TCTTACGGAA ATTTGAGAAT AAAATTAATT TAGATAAGCT AAATGTTACT 
GATTCCGTCA TAAATAAAGT CACCGAAAAG TCTAGACAAA AGGAAGCAGA TATGTATCGC 
ATCAAAGATA AGGCAGACAG AGCAACTGTA GAACAGGTGT TGGATCCCAG AACAAGAATG 
ATTTTATTCA AGATGTTGAC TAGAGGAATC ATAACAGAGA TAAATGGCTG CATTAGCACA 
GGAAAAGAAG CTAATGTATA CCATGCTAGC ACAGCAAATG GAGAGAGCAG AGCAATCAAA 
ATTTATAAAA CTTCTATTTT GGTGTTCAAA GATCGGGATA AATATGTAAG TGGAGAATTC 
AGATTTCGTC ATGGCTATTG TAAAGGAAAC CCTAGGAAAA TGGTGAAAAC TTGGGCAGAA 
AAAGAAATGA GGAACTTAAT CAGGCTAAAC ACAGCAGAGA TACCATGTCC AGAACCAATA 
ATGCTAAGAA GTCATGTTCT TGTCATGAGT TTCATCGGTA AAGATGACAT GCCTGCACCA 
CTCTTGAAAA ATGTCCAGTT ATCAGAATCC AAGGCTCGGG AGTTGTACCT GCAGGTCATT 
CAGTACATGA GAAGAATGTA TCAGGATGCC AGACTTGTCC ATGCAGATCT CAGTGAATTT 
AACATGCTGT ACCACGGTGG AGGCGTGTAT ATCATTGACG TGTCTCAGTC CGTGGAGCAC 
GACCACCCAC ATGCCTTGGA GTTCTTGAGA AAGGATTGCG CCAACGTCAA TGATTTCTTT 
ATGAGGCACA GTGTTGCTGT CATGACTGTG CGGGAGCTCT TTGAATTTGT CACAGATCCA 
TCCATTACAC ATGAGAACAT GGATGCTTAT CTCTCAAAGG CCATGGAAAT AGCATCTCAA 
AGGACCAAGG AAGAACGGTC TAGCCAAGAT CATGTGGATG AAGAGGTGTT TAAGCGAGCA 
TATATTCCTA GAACCTTGAA TGAAGTGAAA AATTATGAGA GGGATATGGA CATAATTATG 
AAATTGAAGG AAGAGGACAT GGCCATGAAT GCCCAACAAG ATAATATTCT ATACCAGACT 
GTTACAGGAT TGAAGAAAGA TTTGTCAGGA GTTCAGAAGG TCCCTGCACT CCTAGAAAAT 
CAAGTGGAGG AAAGGACTTG TTCTGATTCA GAAGATATTG GAAGCTCTGA GTGCTCTGAC 
ACAGACTCTG AAGAGCAGGG AGACCATGCC CGCCCCAAGA AACACACCAC GGACCCTGAC 
ATTGATAAAA AAGAAAGAAA AAAGATGGTC AAGGAAGCCC AGAGAGAGAA AAGAAAAAAC 
AAAATTCCTA AACATGTGAA AAAAAGAAAG GAGAAGACAG CCAAGACGAA AAAAGGCAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513235.2
CDS	101..1843
Translation	MCFLNSRLWAWRMSKQLQQNWKTGEHGFKEMELIGSENRDLKTVKEKDDILFEDLQDNVNENGEGEIEDEEEEGYDDDDDDWDWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSRQKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHASTANGESRAIKIYKTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK

Target ORF information:

RefSeq Version	XM_011514933.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RIO kinase 1 (RIOK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514933.2

ORF Insert Sequence:

ATGTGCTTCT TGAATAGTAG GCTTTGGGCA TGGAGAATGA GCAAACAACT GCAACAGAAC 
TGGAAAACAG GAGAACATGG TTTTAAGGAG ATGGAGCTTA TCGGAAGTGA AAACAGAGAC 
TTGAAGACAG TCAAAGAGAA GGATGACATT CTGTTTGAAG ACCTTCAAGA CAATGTGAAT 
GAGAATGGTG AAGGTGAAAT AGAAGATGAG GAGGAGGAGG GTTATGACGA TGATGATGAT 
GACTGGGACT GGGATGAAGG AGTTGGAAAA CTCGCCAAGG GTTATGTCTG GAATGGAGGA 
AGCAACCCAC AGGCAAATCG ACAGACCTCC GACAGCAGTT CAGCCAAAAT GTCTACTCCA 
GCAGACAAGG TCTTACGGAA ATTTGAGAAT AAAATTAATT TAGATAAGCT AAATGTTACT 
GATTCCGTCA TAAATAAAGT CACCGAAAAG TCTAGACAAA AGGAAGCAGA TATGTATCGC 
ATCAAAGATA AGGCAGACAG AGCAACTGTA GAACAGGTGT TGGATCCCAG AACAAGAATG 
ATTTTATTCA AGATGTTGAC TAGAGGAATC ATAACAGAGA TAAATGGCTG CATTAGCACA 
GGAAAAGAAG CTAATGTATA CCATGCTAGC ACAGCAAATG GAGAGAGCAG AGCAATCAAA 
ATTTATAAAA CTTCTATTTT GGTGTTCAAA GATCGGGATA AATATGTAAG TGGAGAATTC 
AGATTTCGTC ATGGCTATTG TAAAGGAAAC CCTAGGAAAA TGGTGAAAAC TTGGGCAGAA 
AAAGAAATGA GGAACTTAAT CAGGCTAAAC ACAGCAGAGA TACCATGTCC AGAACCAATA 
ATGCTAAGAA GTCATGTTCT TGTCATGAGT TTCATCGGTA AAGATGACAT GCCTGCACCA 
CTCTTGAAAA ATGTCCAGTT ATCAGAATCC AAGGCTCGGG AGTTGTACCT GCAGGTCATT 
CAGTACATGA GAAGAATGTA TCAGGATGCC AGACTTGTCC ATGCAGATCT CAGTGAATTT 
AACATGCTGT ACCACGGTGG AGGCGTGTAT ATCATTGACG TGTCTCAGTC CGTGGAGCAC 
GACCACCCAC ATGCCTTGGA GTTCTTGAGA AAGGATTGCG CCAACGTCAA TGATTTCTTT 
ATGAGGCACA GTGTTGCTGT CATGACTGTG CGGGAGCTCT TTGAATTTGT CACAGATCCA 
TCCATTACAC ATGAGAACAT GGATGCTTAT CTCTCAAAGG CCATGGAAAT AGCATCTCAA 
AGGACCAAGG AAGAACGGTC TAGCCAAGAT CATGTGGATG AAGAGGTGTT TAAGCGAGCA 
TATATTCCTA GAACCTTGAA TGAAGTGAAA AATTATGAGA GGGATATGGA CATAATTATG 
AAATTGAAGG AAGAGGACAT GGCCATGAAT GCCCAACAAG ATAATATTCT ATACCAGACT 
GTTACAGGAT TGAAGAAAGA TTTGTCAGGA GTTCAGAAGG TCCCTGCACT CCTAGAAAAT 
CAAGTGGAGG AAAGGACTTG TTCTGATTCA GAAGATATTG GAAGCTCTGA GTGCTCTGAC 
ACAGACTCTG AAGAGCAGGG AGACCATGCC CGCCCCAAGA AACACACCAC GGACCCTGAC 
ATTGATAAAA AAGAAAGAAA AAAGATGGTC AAGGAAGCCC AGAGAGAGAA AAGAAAAAAC 
AAAATTCCTA AACATGTGAA AAAAAGAAAG GAGAAGACAG CCAAGACGAA AAAAGGCAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Atypical Kinase RIOK1 Promotes Tumor Growth and Invasive Behavior.
EBioMedicine2079-97(2017 Jun)
Weinberg F,Reischmann N,Fauth L,Taromi S,Mastroianni J,K?hler M,Halbach S,Becker AC,Deng N,Schmitz T,Uhl FM,Herbener N,Riedel B,Beier F,Swarbrick A,Lassmann S,Dengjel J,Zeiser R,Brummer T

Benzimidazole inhibitors of protein kinase CK2 potently inhibit the activity of atypical protein kinase Rio1.
Molecular and cellular biochemistry426(1-2)195-203(2017 Feb)
Kubi?ski K,Mas?yk M,Orzeszko A

MTAP Deletions in Cancer Create Vulnerability to Targeting of the MAT2A/PRMT5/RIOK1 Axis.
Cell reports15(3)574-587(2016 Apr)
Marjon K,Cameron MJ,Quang P,Clasquin MF,Mandley E,Kunii K,McVay M,Choe S,Kernytsky A,Gross S,Konteatis Z,Murtie J,Blake ML,Travins J,Dorsch M,Biller SA,Marks KM

Dominant Rio1 kinase/ATPase catalytic mutant induces trapping of late pre-40S biogenesis factors in 80S-like ribosomes.
Nucleic acids research42(13)8635-47(2014 Jul)
Ferreira-Cerca S,Kiburu I,Thomson E,LaRonde N,Hurt E

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RIOK1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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