Ddah1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddah1
Entrez Gene ID	69219
Full Name	dimethylarginine dimethylaminohydrolase 1
Synonyms	2410006N07Rik,2510015N06Rik,AI987801,AW050362
General protein information	Preferred Names dimethylarginine dimethylaminohydrolase 1 Names N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 DDAH-1 DDAHI dimethylargininase-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 H2

mRNA and Protein(s)

mRNA	Protein	Name
XM_017319712.1	XP_017175201.1	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X1
XM_017319712.2	XP_017175201.1	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X1
XM_011240221.3	XP_011238523.1	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X2
NM_026993.3	NP_081269.1	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
XM_011240221.2	XP_011238523.1	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X2
XM_030252803.1	XP_030108663.1	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X2

Pathways from BioSystems

Homologies

	DDAH1	XP_001108967.1
Drosophila melanogaster (fruit fly)	CG1764	NP_572871.1
Homo sapiens (human)	DDAH1	NP_036269.1
Mus musculus (house mouse)	Ddah1	NP_081269.1
Danio rerio (zebrafish)	zgc:85829	NP_998441.1
Xenopus tropicalis (tropical clawed frog)	ddah1	NP_001120524.1
Gallus gallus (chicken)	DDAH1	NP_989713.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001644	XP_003435894.1
Pan troglodytes (chimpanzee)	DDAH1	XP_001142408.1
Canis lupus familiaris (dog)	DDAH1	XP_547303.1
Bos taurus (cattle)	DDAH1	NP_001095671.1
Rattus norvegicus (Norway rat)	Ddah1	NP_071633.1

Gene Ontology
Bibliography

Related articles in PubMed

Cardiomyocyte dimethylarginine dimethylaminohydrolase-1 (DDAH1) plays an important role in attenuating ventricular hypertrophy and dysfunction.
Xu X, Zhang P, Kwak D, Fassett J, Yue W, Atzler D, Hu X, Liu X, Wang H, Lu Z, Guo H, Schwedhelm E, B?ger RH, Chen P, Chen Y
Basic research in cardiology112(5)55(2017 Aug)

Dimethylarginine Dimethylaminohydrolase 1 Protects Against High-Fat Diet-Induced Hepatic Steatosis and Insulin Resistance in Mice.
Li T, Feng R, Zhao C, Wang Y, Wang J, Liu S, Cao J, Wang H, Wang T, Guo Y, Lu Z
Antioxidants & redox signaling26(11)598-609(2017 Apr)

MCD diet-induced steatohepatitis is associated with alterations in asymmetric dimethylarginine (ADMA) and its transporters.
Di Pasqua LG, Berardo C, Rizzo V, Richelmi P, Croce AC, Vairetti M, Ferrigno A
Molecular and cellular biochemistry419(1-2)147-55(2016 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the ADMA/DDAH1 pathway has a marked effect on hepatic lipogenesis and steatosis induced by HFD feeding. Our findings suggest that strategies to increase DDAH1 activity in hepatocytes may provide a novel approach to attenuate NAFLD development.
Title: Dimethylarginine Dimethylaminohydrolase 1 Protects Against High-Fat Diet-Induced Hepatic Steatosis and Insulin Resistance in Mice.

A time-dependent decrease in serum and tissue ADMA and increase in mRNA expression of DDAH-1 and PRMT-1 as well as higher rates of mRNA expression of CAT-1 and lower rates of CAT-2A and CAT-2B were found after 8-week MCD diet.
Title: MCD diet-induced steatohepatitis is associated with alterations in asymmetric dimethylarginine (ADMA) and its transporters.

In mild CKD, dysregulation of the ADMA/DDAH pathway in adipose tissue triggers lipodystrophy-like phenotype changes, including ectopic fat depositions.
Title: The role of adipose tissue asymmetric dimethylarginine/dimethylarginine dimethylaminohydrolase pathway in adipose tissue phenotype and metabolic abnormalities in subtotally nephrectomized rats.

our results suggest that DDAH1 not only acts as an enzyme degrading ADMA but also controls cellular oxidative stress and apoptosis via a miR-21-dependent pathway.
Title: DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts.

transgenic mice display attenuated cigarette smoke-induced lung inflammation
Title: Asymmetric dimethyl-arginine metabolism in a murine model of cigarette smoke-mediated lung inflammation.

The following Ddah1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddah1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21575	NM_026993.3 Latest version!	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu70400	XM_030252803.1 Latest version!	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu70400	XM_011240221.3 Latest version!	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu80695	XM_017319712.2 Latest version!	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu80695	XM_017319712.1	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu70400	XM_011240221.2	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddah1 ( XM_011240221.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238523.1 Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu70400
Clone ID Related Accession (Same CDS sequence)	XM_011240221.3 , XM_011240221.2 , XM_030252803.1
Accession Version	XM_011240221.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240221.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAG AGGCTTTGGA AAAACTTCAG CTCAACATAG TAGAGATGAA AGATGAAAAT 
GCAACTTTGG ATGGTGGGGA CGTCCTATTC ACAGGCAGAG AATTTTTTGT GGGCCTTTCC 
AAAAGAACAA ATCAACGAGG TGCTGAAATC TTGGCTGATA CTTTTAAGGA CTACGCAGTC 
TCTACAGTCC CTGTGGCCGA TTCTTTGCAT TTAAAGAGTT TCTGCAGCAT GGCCGGACCC 
AACCTGATTG CAATAGGGTC CAGCGAATCT GCACAGAAGG CCCTCAAGAT CATGCAACAG 
ATGAGTGACC ATCGTTATGA CAAGCTCACT GTACCCGACG ACATGGCCGC CAACTGCATA 
TATCTAAATA TCCCCAGCAA AGGGCATGTC TTGCTGCACC GAACCCCAGA AGAGTACCCA 
GAAAGCGCAA AGGTCTATGA GAAACTCAAG GACCATCTAC TGATCCCTGT GAGCAACTCG 
GAGATGGAAA AGGTGGACGG CTTGCTCACC TGCTGCTCCG TTTTTATTAA CAAGAAGATA 
GACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238523.1
CDS	31..579
Translation	MMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADSLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDMAANCIYLNIPSKGHVLLHRTPEEYPESAKVYEKLKDHLLIPVSNSEMEKVDGLLTCCSVFINKKIDS

Target ORF information:

RefSeq Version	XM_011240221.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240221.2

ORF Insert Sequence:

ATGATGAAAG AGGCTTTGGA AAAACTTCAG CTCAACATAG TAGAGATGAA AGATGAAAAT 
GCAACTTTGG ATGGTGGGGA CGTCCTATTC ACAGGCAGAG AATTTTTTGT GGGCCTTTCC 
AAAAGAACAA ATCAACGAGG TGCTGAAATC TTGGCTGATA CTTTTAAGGA CTACGCAGTC 
TCTACAGTCC CTGTGGCCGA TTCTTTGCAT TTAAAGAGTT TCTGCAGCAT GGCCGGACCC 
AACCTGATTG CAATAGGGTC CAGCGAATCT GCACAGAAGG CCCTCAAGAT CATGCAACAG 
ATGAGTGACC ATCGTTATGA CAAGCTCACT GTACCCGACG ACATGGCCGC CAACTGCATA 
TATCTAAATA TCCCCAGCAA AGGGCATGTC TTGCTGCACC GAACCCCAGA AGAGTACCCA 
GAAAGCGCAA AGGTCTATGA GAAACTCAAG GACCATCTAC TGATCCCTGT GAGCAACTCG 
GAGATGGAAA AGGTGGACGG CTTGCTCACC TGCTGCTCCG TTTTTATTAA CAAGAAGATA 
GACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddah1 ( NM_026993.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081269.1 Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), mRNA.

Price & Availability↑

CloneID	OMu21575
Clone ID Related Accession (Same CDS sequence)	NM_026993.3
Accession Version	NM_026993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK148907.1, AK149185.1 and AK010970.1. On Dec 2, 2008 this sequence version replaced NM_026993.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK149185.1, SRR1660813.239923.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGCC TCGGCCACCC CTCCGCCTTC GGCCGGGCCA CCCACGCCGT GGTGCGGGCT 
CCGCCCGAGT CCCTGTGCCG CCACGCGCTG AGGCGCTCGC AGGGCGAGGA GGTGGATTTC 
GCTCGCGCCG AGCGCCAGCA CGAGCTCTAC GTGGGCGTGC TGGGCAGCAA GCTGGGGCTG 
CAGGTGGTGC AGCTGCCCGC CGACGAGAGC CTGCCCGACT GCGTGTTCGT GGAGGACGTG 
GCCGTCGTGT GCGAGGAGAC GGCCCTCATC ACCCGCCCCG GGGCGCCCAG CCGCAGGAAG 
GAGGTTGACA TGATGAAAGA GGCTTTGGAA AAACTTCAGC TCAACATAGT AGAGATGAAA 
GATGAAAATG CAACTTTGGA TGGTGGGGAC GTCCTATTCA CAGGCAGAGA ATTTTTTGTG 
GGCCTTTCCA AAAGAACAAA TCAACGAGGT GCTGAAATCT TGGCTGATAC TTTTAAGGAC 
TACGCAGTCT CTACAGTCCC TGTGGCCGAT TCTTTGCATT TAAAGAGTTT CTGCAGCATG 
GCCGGACCCA ACCTGATTGC AATAGGGTCC AGCGAATCTG CACAGAAGGC CCTCAAGATC 
ATGCAACAGA TGAGTGACCA TCGTTATGAC AAGCTCACTG TACCCGACGA CATGGCCGCC 
AACTGCATAT ATCTAAATAT CCCCAGCAAA GGGCATGTCT TGCTGCACCG AACCCCAGAA 
GAGTACCCAG AAAGCGCAAA GGTCTATGAG AAACTCAAGG ACCATCTACT GATCCCTGTG 
AGCAACTCGG AGATGGAAAA GGTGGACGGC TTGCTCACCT GCTGCTCCGT TTTTATTAAC 
AAGAAGATAG ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081269.1
CDS	171..1028
Translation	MAGLGHPSAFGRATHAVVRAPPESLCRHALRRSQGEEVDFARAERQHELYVGVLGSKLGLQVVQLPADESLPDCVFVEDVAVVCEETALITRPGAPSRRKEVDMMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADSLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDMAANCIYLNIPSKGHVLLHRTPEEYPESAKVYEKLKDHLLIPVSNSEMEKVDGLLTCCSVFINKKIDS

Target ORF information:

RefSeq Version	NM_026993.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026993.3

ORF Insert Sequence:

ATGGCCGGCC TCGGCCACCC CTCCGCCTTC GGCCGGGCCA CCCACGCCGT GGTGCGGGCT 
CCGCCCGAGT CCCTGTGCCG CCACGCGCTG AGGCGCTCGC AGGGCGAGGA GGTGGATTTC 
GCTCGCGCCG AGCGCCAGCA CGAGCTCTAC GTGGGCGTGC TGGGCAGCAA GCTGGGGCTG 
CAGGTGGTGC AGCTGCCCGC CGACGAGAGC CTGCCCGACT GCGTGTTCGT GGAGGACGTG 
GCCGTCGTGT GCGAGGAGAC GGCCCTCATC ACCCGCCCCG GGGCGCCCAG CCGCAGGAAG 
GAGGTTGACA TGATGAAAGA GGCTTTGGAA AAACTTCAGC TCAACATAGT AGAGATGAAA 
GATGAAAATG CAACTTTGGA TGGTGGGGAC GTCCTATTCA CAGGCAGAGA ATTTTTTGTG 
GGCCTTTCCA AAAGAACAAA TCAACGAGGT GCTGAAATCT TGGCTGATAC TTTTAAGGAC 
TACGCAGTCT CTACAGTCCC TGTGGCCGAT TCTTTGCATT TAAAGAGTTT CTGCAGCATG 
GCCGGACCCA ACCTGATTGC AATAGGGTCC AGCGAATCTG CACAGAAGGC CCTCAAGATC 
ATGCAACAGA TGAGTGACCA TCGTTATGAC AAGCTCACTG TACCCGACGA CATGGCCGCC 
AACTGCATAT ATCTAAATAT CCCCAGCAAA GGGCATGTCT TGCTGCACCG AACCCCAGAA 
GAGTACCCAG AAAGCGCAAA GGTCTATGAG AAACTCAAGG ACCATCTACT GATCCCTGTG 
AGCAACTCGG AGATGGAAAA GGTGGACGGC TTGCTCACCT GCTGCTCCGT TTTTATTAAC 
AAGAAGATAG ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddah1 ( XM_011240221.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238523.1 Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu70400
Clone ID Related Accession (Same CDS sequence)	XM_011240221.3 , XM_011240221.2 , XM_030252803.1
Accession Version	XM_011240221.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240221.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAG AGGCTTTGGA AAAACTTCAG CTCAACATAG TAGAGATGAA AGATGAAAAT 
GCAACTTTGG ATGGTGGGGA CGTCCTATTC ACAGGCAGAG AATTTTTTGT GGGCCTTTCC 
AAAAGAACAA ATCAACGAGG TGCTGAAATC TTGGCTGATA CTTTTAAGGA CTACGCAGTC 
TCTACAGTCC CTGTGGCCGA TTCTTTGCAT TTAAAGAGTT TCTGCAGCAT GGCCGGACCC 
AACCTGATTG CAATAGGGTC CAGCGAATCT GCACAGAAGG CCCTCAAGAT CATGCAACAG 
ATGAGTGACC ATCGTTATGA CAAGCTCACT GTACCCGACG ACATGGCCGC CAACTGCATA 
TATCTAAATA TCCCCAGCAA AGGGCATGTC TTGCTGCACC GAACCCCAGA AGAGTACCCA 
GAAAGCGCAA AGGTCTATGA GAAACTCAAG GACCATCTAC TGATCCCTGT GAGCAACTCG 
GAGATGGAAA AGGTGGACGG CTTGCTCACC TGCTGCTCCG TTTTTATTAA CAAGAAGATA 
GACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238523.1
CDS	105..653
Translation	MMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADSLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDMAANCIYLNIPSKGHVLLHRTPEEYPESAKVYEKLKDHLLIPVSNSEMEKVDGLLTCCSVFINKKIDS

Target ORF information:

RefSeq Version	XM_011240221.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240221.3

ORF Insert Sequence:

ATGATGAAAG AGGCTTTGGA AAAACTTCAG CTCAACATAG TAGAGATGAA AGATGAAAAT 
GCAACTTTGG ATGGTGGGGA CGTCCTATTC ACAGGCAGAG AATTTTTTGT GGGCCTTTCC 
AAAAGAACAA ATCAACGAGG TGCTGAAATC TTGGCTGATA CTTTTAAGGA CTACGCAGTC 
TCTACAGTCC CTGTGGCCGA TTCTTTGCAT TTAAAGAGTT TCTGCAGCAT GGCCGGACCC 
AACCTGATTG CAATAGGGTC CAGCGAATCT GCACAGAAGG CCCTCAAGAT CATGCAACAG 
ATGAGTGACC ATCGTTATGA CAAGCTCACT GTACCCGACG ACATGGCCGC CAACTGCATA 
TATCTAAATA TCCCCAGCAA AGGGCATGTC TTGCTGCACC GAACCCCAGA AGAGTACCCA 
GAAAGCGCAA AGGTCTATGA GAAACTCAAG GACCATCTAC TGATCCCTGT GAGCAACTCG 
GAGATGGAAA AGGTGGACGG CTTGCTCACC TGCTGCTCCG TTTTTATTAA CAAGAAGATA 
GACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddah1 ( XM_030252803.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108663.1 Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu70400
Clone ID Related Accession (Same CDS sequence)	XM_011240221.3 , XM_011240221.2 , XM_030252803.1
Accession Version	XM_030252803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAG AGGCTTTGGA AAAACTTCAG CTCAACATAG TAGAGATGAA AGATGAAAAT 
GCAACTTTGG ATGGTGGGGA CGTCCTATTC ACAGGCAGAG AATTTTTTGT GGGCCTTTCC 
AAAAGAACAA ATCAACGAGG TGCTGAAATC TTGGCTGATA CTTTTAAGGA CTACGCAGTC 
TCTACAGTCC CTGTGGCCGA TTCTTTGCAT TTAAAGAGTT TCTGCAGCAT GGCCGGACCC 
AACCTGATTG CAATAGGGTC CAGCGAATCT GCACAGAAGG CCCTCAAGAT CATGCAACAG 
ATGAGTGACC ATCGTTATGA CAAGCTCACT GTACCCGACG ACATGGCCGC CAACTGCATA 
TATCTAAATA TCCCCAGCAA AGGGCATGTC TTGCTGCACC GAACCCCAGA AGAGTACCCA 
GAAAGCGCAA AGGTCTATGA GAAACTCAAG GACCATCTAC TGATCCCTGT GAGCAACTCG 
GAGATGGAAA AGGTGGACGG CTTGCTCACC TGCTGCTCCG TTTTTATTAA CAAGAAGATA 
GACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108663.1
CDS	88..636
Translation	MMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADSLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDMAANCIYLNIPSKGHVLLHRTPEEYPESAKVYEKLKDHLLIPVSNSEMEKVDGLLTCCSVFINKKIDS

Target ORF information:

RefSeq Version	XM_030252803.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252803.1

ORF Insert Sequence:

ATGATGAAAG AGGCTTTGGA AAAACTTCAG CTCAACATAG TAGAGATGAA AGATGAAAAT 
GCAACTTTGG ATGGTGGGGA CGTCCTATTC ACAGGCAGAG AATTTTTTGT GGGCCTTTCC 
AAAAGAACAA ATCAACGAGG TGCTGAAATC TTGGCTGATA CTTTTAAGGA CTACGCAGTC 
TCTACAGTCC CTGTGGCCGA TTCTTTGCAT TTAAAGAGTT TCTGCAGCAT GGCCGGACCC 
AACCTGATTG CAATAGGGTC CAGCGAATCT GCACAGAAGG CCCTCAAGAT CATGCAACAG 
ATGAGTGACC ATCGTTATGA CAAGCTCACT GTACCCGACG ACATGGCCGC CAACTGCATA 
TATCTAAATA TCCCCAGCAA AGGGCATGTC TTGCTGCACC GAACCCCAGA AGAGTACCCA 
GAAAGCGCAA AGGTCTATGA GAAACTCAAG GACCATCTAC TGATCCCTGT GAGCAACTCG 
GAGATGGAAA AGGTGGACGG CTTGCTCACC TGCTGCTCCG TTTTTATTAA CAAGAAGATA 
GACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddah1 ( XM_017319712.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175201.1 Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80695
Clone ID Related Accession (Same CDS sequence)	XM_017319712.1 , XM_017319712.2
Accession Version	XM_017319712.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTCC AGAAGCGGTC CCGTTGCCGT GTTCTCGGGC ACTTACTTGT TCAGGTTGAC 
ATGATGAAAG AGGCTTTGGA AAAACTTCAG CTCAACATAG TAGAGATGAA AGATGAAAAT 
GCAACTTTGG ATGGTGGGGA CGTCCTATTC ACAGGCAGAG AATTTTTTGT GGGCCTTTCC 
AAAAGAACAA ATCAACGAGG TGCTGAAATC TTGGCTGATA CTTTTAAGGA CTACGCAGTC 
TCTACAGTCC CTGTGGCCGA TTCTTTGCAT TTAAAGAGTT TCTGCAGCAT GGCCGGACCC 
AACCTGATTG CAATAGGGTC CAGCGAATCT GCACAGAAGG CCCTCAAGAT CATGCAACAG 
ATGAGTGACC ATCGTTATGA CAAGCTCACT GTACCCGACG ACATGGCCGC CAACTGCATA 
TATCTAAATA TCCCCAGCAA AGGGCATGTC TTGCTGCACC GAACCCCAGA AGAGTACCCA 
GAAAGCGCAA AGGTCTATGA GAAACTCAAG GACCATCTAC TGATCCCTGT GAGCAACTCG 
GAGATGGAAA AGGTGGACGG CTTGCTCACC TGCTGCTCCG TTTTTATTAA CAAGAAGATA 
GACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175201.1
CDS	58..666
Translation	MSVQKRSRCRVLGHLLVQVDMMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADSLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDMAANCIYLNIPSKGHVLLHRTPEEYPESAKVYEKLKDHLLIPVSNSEMEKVDGLLTCCSVFINKKIDS

Target ORF information:

RefSeq Version	XM_017319712.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319712.1

ORF Insert Sequence:

ATGTCAGTCC AGAAGCGGTC CCGTTGCCGT GTTCTCGGGC ACTTACTTGT TCAGGTTGAC 
ATGATGAAAG AGGCTTTGGA AAAACTTCAG CTCAACATAG TAGAGATGAA AGATGAAAAT 
GCAACTTTGG ATGGTGGGGA CGTCCTATTC ACAGGCAGAG AATTTTTTGT GGGCCTTTCC 
AAAAGAACAA ATCAACGAGG TGCTGAAATC TTGGCTGATA CTTTTAAGGA CTACGCAGTC 
TCTACAGTCC CTGTGGCCGA TTCTTTGCAT TTAAAGAGTT TCTGCAGCAT GGCCGGACCC 
AACCTGATTG CAATAGGGTC CAGCGAATCT GCACAGAAGG CCCTCAAGAT CATGCAACAG 
ATGAGTGACC ATCGTTATGA CAAGCTCACT GTACCCGACG ACATGGCCGC CAACTGCATA 
TATCTAAATA TCCCCAGCAA AGGGCATGTC TTGCTGCACC GAACCCCAGA AGAGTACCCA 
GAAAGCGCAA AGGTCTATGA GAAACTCAAG GACCATCTAC TGATCCCTGT GAGCAACTCG 
GAGATGGAAA AGGTGGACGG CTTGCTCACC TGCTGCTCCG TTTTTATTAA CAAGAAGATA 
GACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddah1 ( XM_017319712.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175201.1 Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X1, mRNA.

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CloneID	OMu80695
Clone ID Related Accession (Same CDS sequence)	XM_017319712.1 , XM_017319712.2
Accession Version	XM_017319712.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTCC AGAAGCGGTC CCGTTGCCGT GTTCTCGGGC ACTTACTTGT TCAGGTTGAC 
ATGATGAAAG AGGCTTTGGA AAAACTTCAG CTCAACATAG TAGAGATGAA AGATGAAAAT 
GCAACTTTGG ATGGTGGGGA CGTCCTATTC ACAGGCAGAG AATTTTTTGT GGGCCTTTCC 
AAAAGAACAA ATCAACGAGG TGCTGAAATC TTGGCTGATA CTTTTAAGGA CTACGCAGTC 
TCTACAGTCC CTGTGGCCGA TTCTTTGCAT TTAAAGAGTT TCTGCAGCAT GGCCGGACCC 
AACCTGATTG CAATAGGGTC CAGCGAATCT GCACAGAAGG CCCTCAAGAT CATGCAACAG 
ATGAGTGACC ATCGTTATGA CAAGCTCACT GTACCCGACG ACATGGCCGC CAACTGCATA 
TATCTAAATA TCCCCAGCAA AGGGCATGTC TTGCTGCACC GAACCCCAGA AGAGTACCCA 
GAAAGCGCAA AGGTCTATGA GAAACTCAAG GACCATCTAC TGATCCCTGT GAGCAACTCG 
GAGATGGAAA AGGTGGACGG CTTGCTCACC TGCTGCTCCG TTTTTATTAA CAAGAAGATA 
GACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175201.1
CDS	254..862
Translation	MSVQKRSRCRVLGHLLVQVDMMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADSLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDMAANCIYLNIPSKGHVLLHRTPEEYPESAKVYEKLKDHLLIPVSNSEMEKVDGLLTCCSVFINKKIDS

Target ORF information:

RefSeq Version	XM_017319712.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dimethylarginine dimethylaminohydrolase 1 (Ddah1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319712.2

ORF Insert Sequence:

ATGTCAGTCC AGAAGCGGTC CCGTTGCCGT GTTCTCGGGC ACTTACTTGT TCAGGTTGAC 
ATGATGAAAG AGGCTTTGGA AAAACTTCAG CTCAACATAG TAGAGATGAA AGATGAAAAT 
GCAACTTTGG ATGGTGGGGA CGTCCTATTC ACAGGCAGAG AATTTTTTGT GGGCCTTTCC 
AAAAGAACAA ATCAACGAGG TGCTGAAATC TTGGCTGATA CTTTTAAGGA CTACGCAGTC 
TCTACAGTCC CTGTGGCCGA TTCTTTGCAT TTAAAGAGTT TCTGCAGCAT GGCCGGACCC 
AACCTGATTG CAATAGGGTC CAGCGAATCT GCACAGAAGG CCCTCAAGAT CATGCAACAG 
ATGAGTGACC ATCGTTATGA CAAGCTCACT GTACCCGACG ACATGGCCGC CAACTGCATA 
TATCTAAATA TCCCCAGCAA AGGGCATGTC TTGCTGCACC GAACCCCAGA AGAGTACCCA 
GAAAGCGCAA AGGTCTATGA GAAACTCAAG GACCATCTAC TGATCCCTGT GAGCAACTCG 
GAGATGGAAA AGGTGGACGG CTTGCTCACC TGCTGCTCCG TTTTTATTAA CAAGAAGATA 
GACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiomyocyte dimethylarginine dimethylaminohydrolase-1 (DDAH1) plays an important role in attenuating ventricular hypertrophy and dysfunction.
Basic research in cardiology112(5)55(2017 Aug)
Xu X,Zhang P,Kwak D,Fassett J,Yue W,Atzler D,Hu X,Liu X,Wang H,Lu Z,Guo H,Schwedhelm E,B?ger RH,Chen P,Chen Y

Dimethylarginine Dimethylaminohydrolase 1 Protects Against High-Fat Diet-Induced Hepatic Steatosis and Insulin Resistance in Mice.
Antioxidants & redox signaling26(11)598-609(2017 Apr)
Li T,Feng R,Zhao C,Wang Y,Wang J,Liu S,Cao J,Wang H,Wang T,Guo Y,Lu Z

MCD diet-induced steatohepatitis is associated with alterations in asymmetric dimethylarginine (ADMA) and its transporters.
Molecular and cellular biochemistry419(1-2)147-55(2016 Aug)
Di Pasqua LG,Berardo C,Rizzo V,Richelmi P,Croce AC,Vairetti M,Ferrigno A

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Ddah1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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