Nmb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nmb
Entrez Gene ID	68039
Full Name	neuromedin B
Synonyms	3110023K12Rik
General protein information	Preferred Names neuromedin B Names neuromedin-B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 D3
Summary	This gene encodes a member of the neuromedin family of neuropeptides. The encoded protein is a precursor that is proteolytically processed to generate a biologically active neuropeptide that plays a role in satiety, reproduction and thermoregulation, as well as in stress, fear and other behavioral responses. This gene encodes distinct isoforms, some or all of which may undergo similar processing to generate the mature protein. [provided by RefSeq, Sep 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_026523.4	NP_080799.1	neuromedin-B isoform 2 preproprotein
NM_001291280.1	NP_001278209.1	neuromedin-B isoform 1 preproprotein
XM_011250898.2	XP_011249200.1	neuromedin-B isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NMB	XP_536200.1
Homo sapiens (human)	NMB	NP_066563.2
Rattus norvegicus (Norway rat)	Nmb	NP_001102619.1
Bos taurus (cattle)	NMB	NP_001068738.1
Pan troglodytes (chimpanzee)	NMB	XP_001162171.1
Mus musculus (house mouse)	Nmb	NP_080799.1

Gene Ontology
Bibliography

Related articles in PubMed

Distinct roles of NMB and GRP in itch transmission.
Wan L, Jin H, Liu XY, Jeffry J, Barry DM, Shen KF, Peng JH, Liu XT, Jin JH, Sun Y, Kim R, Meng QT, Mo P, Yin J, Tao A, Bardoni R, Chen ZF
Scientific reports7(1)15466(2017 Nov)

Neuromedin B and its receptor silencing suppresses osteoclast generation by modulating precursor proliferation via M-CSF/c-Fms/D-type cyclins.
Yeo CE, Kang WY, Seong SJ, Cho S, Lee HW, Yoon YR, Kim HJ
Experimental cell research359(1)112-119(2017 10)

Blood-brain barrier transport kinetics of the neuromedin peptides NMU, NMN, NMB and NT.
Gevaert B, Wynendaele E, Stalmans S, Bracke N, D'Hondt M, Smolders I, van Eeckhaut A, De Spiegeleer B
Neuropharmacology107460-470(2016 08)

The peptidergic control circuit for sighing.
Li P, Janczewski WA, Yackle K, Kam K, Pagliardini S, Krasnow MA, Feldman JL
Nature530(7590)293-297(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NMB or NMBR silencing inhibited M-CSF/c-Fms-mediated downstream signaling pathways like activation of ERK and Akt and induction of D-type cyclins, cyclin D1 and D2.
Title: Neuromedin B and its receptor silencing suppresses osteoclast generation by modulating precursor proliferation via M-CSF/c-Fms/D-type cyclins.

Data show that neuromedin B (NMB) expression was higher in the melanocortin 2 receptor MC2R(-/-) than in the wild-type (WT) mice.
Title: Expression and regulation of neuromedin B in pituitary corticotrophs of male melanocortin 2 receptor-deficient mice.

The majority of dorsal spinal cord Grp is synthesized locally in dorsal spinal cord neurons while Nmb is highly expressed in pain- and itch-sensing dorsal root ganglion neurons.
Title: The majority of dorsal spinal cord gastrin releasing peptide is synthesized locally whereas neuromedin B is highly expressed in pain- and itch-sensing somatosensory neurons.

The data demonstrated that the neuromedin B-neuromedian B receptor interaction in pregnant myometrial primary cells in vitro predominantly influenced uterine activity through regulation of Il6 expression via the Rela/p65 pathway.
Title: Neuromedin B and its receptor influence the activity of myometrial primary cells in vitro through regulation of Il6 expression via the Rela/p65 pathway in mice.

REsults describe the expression of neuromedin B in adipose tissue and its regulation by changes in energy balance.
Title: Expression of neuromedin B in adipose tissue and its regulation by changes in energy balance.

The following Nmb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nmb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16469	NM_026523.4 Latest version!	Mus musculus neuromedin B (Nmb), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu16472	NM_001291280.1 Latest version!	Mus musculus neuromedin B (Nmb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu16472	XM_011250898.2 Latest version!	Mus musculus neuromedin B (Nmb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nmb ( NM_026523.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080799.1 Mus musculus neuromedin B (Nmb), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16469
Clone ID Related Accession (Same CDS sequence)	NM_026523.4
Accession Version	NM_026523.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	neuromedin-B isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB058776.1 and AC123744.8. On Apr 1, 2014 this sequence version replaced NM_026523.3. Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of the last exon compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB058776.1, AK011929.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164136, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCGGC AAGCAGGGAG CTCTTGGCTC CTCCGTGGTC TCCTGCTCTT CGCATTGTTC 
GCTTCCGGCG TCGCTCCCTT CAACTGGGAT CTCCCGGAGC CCCGCAGCCG AGCAAGCAAG 
ATTCGAGTGC ACCCTCGGGG CAACCTCTGG GCGACCGGTC ACTTCATGGG CAAGAAGAGC 
CTGGAACCCC CGAGCCTGTC ACTGGTGGGG ACAGCACCCC CTAACACCCC GAGGGACCAG 
AGACTACAGC TGAGTCATGA TCTGCTCAGG ATCCTCCTGC GAAAGAAAGC TCTAGGCATG 
AACTTCAGTG GCCCAGCTCC CCCAATCCAG TACAGGAGGC TGCTGGAGCC ACTACTGCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080799.1
CDS	86..451
Translation	MTRQAGSSWLLRGLLLFALFASGVAPFNWDLPEPRSRASKIRVHPRGNLWATGHFMGKKSLEPPSLSLVGTAPPNTPRDQRLQLSHDLLRILLRKKALGMNFSGPAPPIQYRRLLEPLLQK

Target ORF information:

RefSeq Version	NM_026523.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuromedin B (Nmb), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026523.4

ORF Insert Sequence:

ATGACCCGGC AAGCAGGGAG CTCTTGGCTC CTCCGTGGTC TCCTGCTCTT CGCATTGTTC 
GCTTCCGGCG TCGCTCCCTT CAACTGGGAT CTCCCGGAGC CCCGCAGCCG AGCAAGCAAG 
ATTCGAGTGC ACCCTCGGGG CAACCTCTGG GCGACCGGTC ACTTCATGGG CAAGAAGAGC 
CTGGAACCCC CGAGCCTGTC ACTGGTGGGG ACAGCACCCC CTAACACCCC GAGGGACCAG 
AGACTACAGC TGAGTCATGA TCTGCTCAGG ATCCTCCTGC GAAAGAAAGC TCTAGGCATG 
AACTTCAGTG GCCCAGCTCC CCCAATCCAG TACAGGAGGC TGCTGGAGCC ACTACTGCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nmb ( NM_001291280.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001278209.1 Mus musculus neuromedin B (Nmb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16472
Clone ID Related Accession (Same CDS sequence)	NM_001291280.1 , XM_011250898.2
Accession Version	NM_001291280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-27
Organism	Mus musculus(house mouse)
Product	neuromedin-B isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB058776.1, AA671747.1 and AC123744.8. On Apr 1, 2014 this sequence version replaced XM_006541136.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF105963.1, AA671747.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCGGC AAGCAGGGAG CTCTTGGCTC CTCCGTGGTC TCCTGCTCTT CGCATTGTTC 
GCTTCCGGCG TCGCTCCCTT CAACTGGGAT CTCCCGGAGC CCCGCAGCCG AGCAAGCAAG 
ATTCGAGTGC ACCCTCGGGG CAACCTCTGG GCGACCGGTC ACTTCATGGG CAAGAAGAGC 
CTGGAACCCC CGAGCCTGTC ACTGGTGGGG ACAGCACCCC CTAACACCCC GAGGGACCAG 
AGACTACAGC TGAGTCATGA TCTGCTCAGG ATCCTCCTGC GAAAGAAAGC TCTAGGCATG 
AACTTCAGTG GCCCAGCTCC CCCAATCCAG GAGGCTGCTG GAGCCACTAC TGCAGAAGTG 
ATGCCAATAA TGGAACAAAC CGGATGCTGG GCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278209.1
CDS	86..481
Translation	MTRQAGSSWLLRGLLLFALFASGVAPFNWDLPEPRSRASKIRVHPRGNLWATGHFMGKKSLEPPSLSLVGTAPPNTPRDQRLQLSHDLLRILLRKKALGMNFSGPAPPIQEAAGATTAEVMPIMEQTGCWA

Target ORF information:

RefSeq Version	NM_001291280.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuromedin B (Nmb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291280.1

ORF Insert Sequence:

ATGACCCGGC AAGCAGGGAG CTCTTGGCTC CTCCGTGGTC TCCTGCTCTT CGCATTGTTC 
GCTTCCGGCG TCGCTCCCTT CAACTGGGAT CTCCCGGAGC CCCGCAGCCG AGCAAGCAAG 
ATTCGAGTGC ACCCTCGGGG CAACCTCTGG GCGACCGGTC ACTTCATGGG CAAGAAGAGC 
CTGGAACCCC CGAGCCTGTC ACTGGTGGGG ACAGCACCCC CTAACACCCC GAGGGACCAG 
AGACTACAGC TGAGTCATGA TCTGCTCAGG ATCCTCCTGC GAAAGAAAGC TCTAGGCATG 
AACTTCAGTG GCCCAGCTCC CCCAATCCAG GAGGCTGCTG GAGCCACTAC TGCAGAAGTG 
ATGCCAATAA TGGAACAAAC CGGATGCTGG GCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nmb ( XM_011250898.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011249200.1 Mus musculus neuromedin B (Nmb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16472
Clone ID Related Accession (Same CDS sequence)	NM_001291280.1 , XM_011250898.2
Accession Version	XM_011250898.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuromedin-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCGGC AAGCAGGGAG CTCTTGGCTC CTCCGTGGTC TCCTGCTCTT CGCATTGTTC 
GCTTCCGGCG TCGCTCCCTT CAACTGGGAT CTCCCGGAGC CCCGCAGCCG AGCAAGCAAG 
ATTCGAGTGC ACCCTCGGGG CAACCTCTGG GCGACCGGTC ACTTCATGGG CAAGAAGAGC 
CTGGAACCCC CGAGCCTGTC ACTGGTGGGG ACAGCACCCC CTAACACCCC GAGGGACCAG 
AGACTACAGC TGAGTCATGA TCTGCTCAGG ATCCTCCTGC GAAAGAAAGC TCTAGGCATG 
AACTTCAGTG GCCCAGCTCC CCCAATCCAG GAGGCTGCTG GAGCCACTAC TGCAGAAGTG 
ATGCCAATAA TGGAACAAAC CGGATGCTGG GCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011249200.1
CDS	5404..5799
Translation	MTRQAGSSWLLRGLLLFALFASGVAPFNWDLPEPRSRASKIRVHPRGNLWATGHFMGKKSLEPPSLSLVGTAPPNTPRDQRLQLSHDLLRILLRKKALGMNFSGPAPPIQEAAGATTAEVMPIMEQTGCWA

Target ORF information:

RefSeq Version	XM_011250898.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuromedin B (Nmb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250898.2

ORF Insert Sequence:

ATGACCCGGC AAGCAGGGAG CTCTTGGCTC CTCCGTGGTC TCCTGCTCTT CGCATTGTTC 
GCTTCCGGCG TCGCTCCCTT CAACTGGGAT CTCCCGGAGC CCCGCAGCCG AGCAAGCAAG 
ATTCGAGTGC ACCCTCGGGG CAACCTCTGG GCGACCGGTC ACTTCATGGG CAAGAAGAGC 
CTGGAACCCC CGAGCCTGTC ACTGGTGGGG ACAGCACCCC CTAACACCCC GAGGGACCAG 
AGACTACAGC TGAGTCATGA TCTGCTCAGG ATCCTCCTGC GAAAGAAAGC TCTAGGCATG 
AACTTCAGTG GCCCAGCTCC CCCAATCCAG GAGGCTGCTG GAGCCACTAC TGCAGAAGTG 
ATGCCAATAA TGGAACAAAC CGGATGCTGG GCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distinct roles of NMB and GRP in itch transmission.
Scientific reports7(1)15466(2017 Nov)
Wan L,Jin H,Liu XY,Jeffry J,Barry DM,Shen KF,Peng JH,Liu XT,Jin JH,Sun Y,Kim R,Meng QT,Mo P,Yin J,Tao A,Bardoni R,Chen ZF

Neuromedin B and its receptor silencing suppresses osteoclast generation by modulating precursor proliferation via M-CSF/c-Fms/D-type cyclins.
Experimental cell research359(1)112-119(2017 10)
Yeo CE,Kang WY,Seong SJ,Cho S,Lee HW,Yoon YR,Kim HJ

Blood-brain barrier transport kinetics of the neuromedin peptides NMU, NMN, NMB and NT.
Neuropharmacology107460-470(2016 08)
Gevaert B,Wynendaele E,Stalmans S,Bracke N,D'Hondt M,Smolders I,van Eeckhaut A,De Spiegeleer B

The peptidergic control circuit for sighing.
Nature530(7590)293-297(2016 Feb)
Li P,Janczewski WA,Yackle K,Kam K,Pagliardini S,Krasnow MA,Feldman JL

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Nmb cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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