Ilf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ilf2
Entrez Gene ID	67781
Full Name	interleukin enhancer binding factor 2
Synonyms	6230405A16Rik,TEG-267,Tex261,Tex267
General protein information	Preferred Names interleukin enhancer binding factor 2 Names interleukin enhancer-binding factor 2 nuclear factor of activated T-cells 45 kDa testis expressed gene 267
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_026374.3	NP_080650.1	interleukin enhancer-binding factor 2

Pathways from BioSystems

Homologies

	ILF2	XP_001111422.1
Mus musculus (house mouse)	Ilf2	NP_080650.1
Gallus gallus (chicken)	ILF2	XP_004948269.1
Pan troglodytes (chimpanzee)	ILF2	XP_001142785.1
Canis lupus familiaris (dog)	ILF2	XP_537263.1
Drosophila melanogaster (fruit fly)	CG5641	NP_650196.1
Danio rerio (zebrafish)	ilf2	NP_998401.1
Homo sapiens (human)	ILF2	NP_004506.2
Bos taurus (cattle)	ILF2	NP_001033276.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001997	XP_321062.4
Caenorhabditis elegans (roundworm)	CELE_R11H6.5	NP_506614.1

Gene Ontology
Bibliography

Related articles in PubMed

NF45 and NF90/NF110 coordinately regulate ESC pluripotency and differentiation.
Ye J, Jin H, Pankov A, Song JS, Blelloch R
RNA (New York, N.Y.)23(8)1270-1284(2017 08)

Overexpression of NF90-NF45 Represses Myogenic MicroRNA Biogenesis, Resulting in Development of Skeletal Muscle Atrophy and Centronuclear Muscle Fibers.
Todaka H, Higuchi T, Yagyu K, Sugiyama Y, Yamaguchi F, Morisawa K, Ono M, Fukushima A, Tsuda M, Taniguchi T, Sakamoto S
Molecular and cellular biology35(13)2295-308(2015 Jul)

NF45 dimerizes with NF90, Zfr and SPNR via a conserved domain that has a nucleotidyltransferase fold.
Wolkowicz UM, Cook AG
Nucleic acids research40(18)9356-68(2012 Oct)

NF45 and NF90 regulate HS4-dependent interleukin-13 transcription in T cells.
Kiesler P, Haynes PA, Shi L, Kao PN, Wysocki VH, Vercelli D
The Journal of biological chemistry285(11)8256-67(2010 Mar)

NF45 and NF90 in murine seminiferous epithelium: potential role in SP-10 gene transcription.
Ranpura SA, Deshmukh U, Reddi PP
Journal of andrology29(2)186-97(2008 Mar-Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data support a model in which NF45, NF90, and NF110 operate in feedback loops that enable them, through both overlapping and independent targets, to help balance the push and pull of pluripotency and differentiation cues.
Title: NF45 and NF90/NF110 coordinately regulate ESC pluripotency and differentiation.

NF90-NF45 complex induces centronuclear myopathy through increased dynamin 2 expression by an NF90-NF45-induced reduction of miR-133a expression in vivo.
Title: Overexpression of NF90-NF45 Represses Myogenic MicroRNA Biogenesis, Resulting in Development of Skeletal Muscle Atrophy and Centronuclear Muscle Fibers.

we propose that NF45 and NF90 have the potential to activate SP-10 gene transcription, and that a chromatin modification event must occur first in order to provide access to these transcription factors.
Title: NF45 and NF90 in murine seminiferous epithelium: potential role in SP-10 gene transcription.

Ilf2 is regulated during meiosis and associated to transcriptionally active chromatin
Title: Ilf2 is regulated during meiosis and associated to transcriptionally active chromatin.

The following Ilf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ilf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02124	NM_026374.3 Latest version!	Mus musculus interleukin enhancer binding factor 2 (Ilf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ilf2 ( NM_026374.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080650.1 Mus musculus interleukin enhancer binding factor 2 (Ilf2), mRNA.

Price & Availability↑

CloneID	OMu02124
Clone ID Related Accession (Same CDS sequence)	NM_026374.3
Accession Version	NM_026374.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	interleukin enhancer-binding factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC160552.13. On Nov 17, 2006 this sequence version replaced NM_026374.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC024718.1, AK078003.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAGGGGTG ACAGAGGACG TGGTCGTGGT GGGCGCTTTG GTTCCAGAGG AGGGCCTGGT 
GGAGGGTTCA GGCCTTTTGT GCCCCATATC CCATTTGATT TTTATTTGTG TGAAATGGCT 
TTTCCCCGGG TCAAGCCAGC ACCTGATGAG ACCTCCTTCA GTGAAGCCTT GCTGAAGAGG 
AATCAAGACC TTGCCCCTAA TTCTGCTGAG CAGGCTTCCA TCCTTTCTTT GGTGACAAAA 
ATCAACAATG TGATTGACAA CTTGATTGTG GCCCCAGGAA CATTTGAAGT GCAAATTGAA 
GAAGTCCGGC AGGTAGGATC ATATAAAAAG GGAACAATGA CTACAGGACA CAATGTGGCT 
GACCTGGTGG TAATACTAAA GATCCTGCCA ACATTGGAAG CTGTTGCTGC CCTGGGGAAC 
AAAGTCGTGG AAAGCCTAAG GGCACAGGAT CCCTCAGAAG TTTTGACCAT GCTGACTAAT 
GAAACTGGCT TTGAAATTAG CTCTTCTGAT GCTACGGTGA AAATTCTCAT TACAACAGTG 
CCCCCCAATC TCCGAAAACT GGATCCAGAA CTCCATTTGG ACATCAAGGT GTTGCAGAGC 
GCCTTAGCAG CCATCCGGCA TGCCCGCTGG TTTGAAGAGA ACGCTTCTCA GTCCACAGTT 
AAAGTTCTCA TAAGATTGCT AAAGGACTTA CGGATTCGTT TTCCTGGCTT TGAGCCTCTC 
ACACCCTGGA TCCTTGACCT ACTTGGGCAC TATGCTGTAA TGAACAACCC CACCAGACAG 
CCTTTGGCCC TGAATGTGGC TTACAGGCGC TGCTTGCAGA TTCTGGCTGC AGGACTGTTC 
TTGCCTGGGT CAGTGGGCAT CACTGACCCG TGTGAGAGTG GCAACTTCAG GGTACACACA 
GTTATGACCC TAGAACAACA GGATATGGTC TGCTACACAG CTCAGACTCT GGTTCGAATT 
CTCTCCCATG GAGGCTTTAG GAAGATCCTT GGCCAGGAGG GGGATGCCAG CTATCTTGCT 
TCTGAAATAT CTACCTGGGA TGGAGTGATT GTGACACCCT CAGAAAAGGC TTATGAGAAG 
CCACCTGAGA AGAAGGAGGG GGAGGAAGAG GAGGAGAACA CAGAAGAACC ACCTCAAGGA 
GAAGAAGAAG AAAGCATGGA GACTCAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080650.1
CDS	41..1213
Translation	MRGDRGRGRGGRFGSRGGPGGGFRPFVPHIPFDFYLCEMAFPRVKPAPDETSFSEALLKRNQDLAPNSAEQASILSLVTKINNVIDNLIVAPGTFEVQIEEVRQVGSYKKGTMTTGHNVADLVVILKILPTLEAVAALGNKVVESLRAQDPSEVLTMLTNETGFEISSSDATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCYTAQTLVRILSHGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEEEENTEEPPQGEEEESMETQE

Target ORF information:

RefSeq Version	NM_026374.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus interleukin enhancer binding factor 2 (Ilf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026374.3

ORF Insert Sequence:

ATGAGGGGTG ACAGAGGACG TGGTCGTGGT GGGCGCTTTG GTTCCAGAGG AGGGCCTGGT 
GGAGGGTTCA GGCCTTTTGT GCCCCATATC CCATTTGATT TTTATTTGTG TGAAATGGCT 
TTTCCCCGGG TCAAGCCAGC ACCTGATGAG ACCTCCTTCA GTGAAGCCTT GCTGAAGAGG 
AATCAAGACC TTGCCCCTAA TTCTGCTGAG CAGGCTTCCA TCCTTTCTTT GGTGACAAAA 
ATCAACAATG TGATTGACAA CTTGATTGTG GCCCCAGGAA CATTTGAAGT GCAAATTGAA 
GAAGTCCGGC AGGTAGGATC ATATAAAAAG GGAACAATGA CTACAGGACA CAATGTGGCT 
GACCTGGTGG TAATACTAAA GATCCTGCCA ACATTGGAAG CTGTTGCTGC CCTGGGGAAC 
AAAGTCGTGG AAAGCCTAAG GGCACAGGAT CCCTCAGAAG TTTTGACCAT GCTGACTAAT 
GAAACTGGCT TTGAAATTAG CTCTTCTGAT GCTACGGTGA AAATTCTCAT TACAACAGTG 
CCCCCCAATC TCCGAAAACT GGATCCAGAA CTCCATTTGG ACATCAAGGT GTTGCAGAGC 
GCCTTAGCAG CCATCCGGCA TGCCCGCTGG TTTGAAGAGA ACGCTTCTCA GTCCACAGTT 
AAAGTTCTCA TAAGATTGCT AAAGGACTTA CGGATTCGTT TTCCTGGCTT TGAGCCTCTC 
ACACCCTGGA TCCTTGACCT ACTTGGGCAC TATGCTGTAA TGAACAACCC CACCAGACAG 
CCTTTGGCCC TGAATGTGGC TTACAGGCGC TGCTTGCAGA TTCTGGCTGC AGGACTGTTC 
TTGCCTGGGT CAGTGGGCAT CACTGACCCG TGTGAGAGTG GCAACTTCAG GGTACACACA 
GTTATGACCC TAGAACAACA GGATATGGTC TGCTACACAG CTCAGACTCT GGTTCGAATT 
CTCTCCCATG GAGGCTTTAG GAAGATCCTT GGCCAGGAGG GGGATGCCAG CTATCTTGCT 
TCTGAAATAT CTACCTGGGA TGGAGTGATT GTGACACCCT CAGAAAAGGC TTATGAGAAG 
CCACCTGAGA AGAAGGAGGG GGAGGAAGAG GAGGAGAACA CAGAAGAACC ACCTCAAGGA 
GAAGAAGAAG AAAGCATGGA GACTCAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NF45 and NF90/NF110 coordinately regulate ESC pluripotency and differentiation.
RNA (New York, N.Y.)23(8)1270-1284(2017 08)
Ye J,Jin H,Pankov A,Song JS,Blelloch R

Overexpression of NF90-NF45 Represses Myogenic MicroRNA Biogenesis, Resulting in Development of Skeletal Muscle Atrophy and Centronuclear Muscle Fibers.
Molecular and cellular biology35(13)2295-308(2015 Jul)
Todaka H,Higuchi T,Yagyu K,Sugiyama Y,Yamaguchi F,Morisawa K,Ono M,Fukushima A,Tsuda M,Taniguchi T,Sakamoto S

NF45 dimerizes with NF90, Zfr and SPNR via a conserved domain that has a nucleotidyltransferase fold.
Nucleic acids research40(18)9356-68(2012 Oct)
Wolkowicz UM,Cook AG

NF45 and NF90 regulate HS4-dependent interleukin-13 transcription in T cells.
The Journal of biological chemistry285(11)8256-67(2010 Mar)
Kiesler P,Haynes PA,Shi L,Kao PN,Wysocki VH,Vercelli D

NF45 and NF90 in murine seminiferous epithelium: potential role in SP-10 gene transcription.
Journal of andrology29(2)186-97(2008 Mar-Apr)
Ranpura SA,Deshmukh U,Reddi PP

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Ilf2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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