Atg12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg12
Entrez Gene ID	67526
Full Name	autophagy related 12
Synonyms	4931423H11Rik,A330058M13Rik,Apg12l,Atg12l
General protein information	Preferred Names autophagy related 12 Names ubiquitin-like protein ATG12 autophagy-related protein 12
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_026217.3	NP_080493.2	ubiquitin-like protein ATG12
XM_006526187.2	XP_006526250.1	ubiquitin-like protein ATG12 isoform X1
XM_006526187.1	XP_006526250.1	ubiquitin-like protein ATG12 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ATG12	NP_001070450.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007296	XP_308525.2
Rattus norvegicus (Norway rat)	Atg12	NP_001033584.1
Homo sapiens (human)	ATG12	NP_004698.3
Pan troglodytes (chimpanzee)	ATG12	NP_001238927.1
Canis lupus familiaris (dog)	ATG12	XP_531866.2
Drosophila melanogaster (fruit fly)	Atg12	NP_648551.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012847	XP_307342.3
Xenopus tropicalis (tropical clawed frog)	LOC100493403	XP_002940007.1
Gallus gallus (chicken)	ATG12	XP_424963.1
Danio rerio (zebrafish)	atg12	NP_001233129.1
Mus musculus (house mouse)	Atg12	NP_080493.2

Gene Ontology
Bibliography

Related articles in PubMed

Induction of mitophagy in the HEI-OC1 auditory cell line and activation of the Atg12/LC3 pathway in the organ of Corti.
Setz C, Benischke AS, Pinho Ferreira Bento AC, Brand Y, Levano S, Paech F, Leitmeyer K, Bodmer D
Hearing research(2018 Jan)

Mir505-3p regulates axonal development via inhibiting the autophagy pathway by targeting Atg12.
Yang K, Yu B, Cheng C, Cheng T, Yuan B, Li K, Xiao J, Qiu Z, Zhou Y
Autophagy13(10)1679-1696(2017 Oct)

Beige Adipocyte Maintenance Is Regulated by Autophagy-Induced Mitochondrial Clearance.
Altshuler-Keylin S, Shinoda K, Hasegawa Y, Ikeda K, Hong H, Kang Q, Yang Y, Perera RM, Debnath J, Kajimura S
Cell metabolism24(3)402-419(2016 Sep)

miR-30b inhibits autophagy to alleviate hepatic ischemia-reperfusion injury via decreasing the Atg12-Atg5 conjugate.
Li SP, He JD, Wang Z, Yu Y, Fu SY, Zhang HM, Zhang JJ, Shen ZY
World journal of gastroenterology22(18)4501-14(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-30b inhibited autophagy to alleviate hepatic ischemia-reperfusion injury by decreasing the Atg12-Atg5 conjugate.
Title: miR-30b inhibits autophagy to alleviate hepatic ischemia-reperfusion injury via decreasing the Atg12-Atg5 conjugate.

ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function.
Title: ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function.

Although genetic deletion of either Atg12 or Atg5 renders POMC neurons autophagy-deficient, mice lacking Atg5 in POMC neurons do not exhibit these phenotypes.
Title: Loss of Atg12, but not Atg5, in pro-opiomelanocortin neurons exacerbates diet-induced obesity.

Atg5-Atg12/Atg16L1 protein complex is required for IFNgamma-mediated host defense against murine norovirus infection.
Title: Nondegradative role of Atg5-Atg12/ Atg16L1 autophagy protein complex in antiviral activity of interferon gamma.

Despite forming the Atg12-Atg5-Atg16L2 complex, Atg16L2 is not recruited to phagophores and is mostly present in the cytosol.
Title: Atg16L2, a novel isoform of mammalian Atg16L that is not essential for canonical autophagy despite forming an Atg12?5-16L2 complex.

The following Atg12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18105	NM_026217.3 Latest version!	Mus musculus autophagy related 12 (Atg12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu54745	XM_006526187.2 Latest version!	Mus musculus autophagy related 12 (Atg12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu54745	XM_006526187.1	Mus musculus autophagy related 12 (Atg12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg12 ( XM_006526187.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526250.1 Mus musculus autophagy related 12 (Atg12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54745
Clone ID Related Accession (Same CDS sequence)	XM_006526187.2 , XM_006526187.1
Accession Version	XM_006526187.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin-like protein ATG12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAG ATTCAGAGGT TGTGCTGCAG CTCCCCTCGG CTCCTGTAGG AGCCGGCGGC 
GAGAGCCTTC CAGAGCTCTC CCCGGAAACA GCCACCCCAG AGCCCCCGTC CTCGGCTGCA 
GTTTCGCCCG GAACGGAGGA ACCTCCCGGA GACACCAAGA AAAAAATTTA TTTATGTGAA 
TCAGTCCTTT GCCCCTTCCC CAGACCAAGA AGTTGGAACT CTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526250.1
CDS	294..521
Translation	MSEDSEVVLQLPSAPVGAGGESLPELSPETATPEPPSSAAVSPGTEEPPGDTKKKIYLCESVLCPFPRPRSWNSI

Target ORF information:

RefSeq Version	XM_006526187.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy related 12 (Atg12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526187.1

ORF Insert Sequence:

ATGTCGGAAG ATTCAGAGGT TGTGCTGCAG CTCCCCTCGG CTCCTGTAGG AGCCGGCGGC 
GAGAGCCTTC CAGAGCTCTC CCCGGAAACA GCCACCCCAG AGCCCCCGTC CTCGGCTGCA 
GTTTCGCCCG GAACGGAGGA ACCTCCCGGA GACACCAAGA AAAAAATTTA TTTATGTGAA 
TCAGTCCTTT GCCCCTTCCC CAGACCAAGA AGTTGGAACT CTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg12 ( NM_026217.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080493.2 Mus musculus autophagy related 12 (Atg12), mRNA.

Price & Availability↑

CloneID	OMu18105
Clone ID Related Accession (Same CDS sequence)	NM_026217.3
Accession Version	NM_026217.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Mus musculus(house mouse)
Product	ubiquitin-like protein ATG12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB853539.1, BC070470.1 and AV295996.1. On Apr 27, 2007 this sequence version replaced NM_026217.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.53835.1, AK008698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAAG ATTCAGAGGT TGTGCTGCAG CTCCCCTCGG CTCCTGTAGG AGCCGGCGGC 
GAGAGCCTTC CAGAGCTCTC CCCGGAAACA GCCACCCCAG AGCCCCCGTC CTCGGCTGCA 
GTTTCGCCCG GAACGGAGGA ACCTCCCGGA GACACCAAGA AAAAAATTGA CATCCTGCTG 
AAGGCTGTAG GAGACACTCC TATAATGAAA ACAAAGAAAT GGGCTGTGGA GCGAACCCGG 
ACCATCCAAG GACTCATTGA CTTCATCAAA AAGTTCCTTA AACTGGTGGC CTCGGAACAG 
TTGTTTATTT ATGTGAATCA GTCCTTTGCC CCTTCCCCAG ACCAAGAAGT TGGAACTCTA 
TATGAGTGTT TTGGCAGTGA TGGTAAACTG GTCCTGCATT ACTGCAAATC CCAGGCATGG 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080493.2
CDS	34..459
Translation	MSEDSEVVLQLPSAPVGAGGESLPELSPETATPEPPSSAAVSPGTEEPPGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG

Target ORF information:

RefSeq Version	NM_026217.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy related 12 (Atg12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026217.3

ORF Insert Sequence:

ATGTCGGAAG ATTCAGAGGT TGTGCTGCAG CTCCCCTCGG CTCCTGTAGG AGCCGGCGGC 
GAGAGCCTTC CAGAGCTCTC CCCGGAAACA GCCACCCCAG AGCCCCCGTC CTCGGCTGCA 
GTTTCGCCCG GAACGGAGGA ACCTCCCGGA GACACCAAGA AAAAAATTGA CATCCTGCTG 
AAGGCTGTAG GAGACACTCC TATAATGAAA ACAAAGAAAT GGGCTGTGGA GCGAACCCGG 
ACCATCCAAG GACTCATTGA CTTCATCAAA AAGTTCCTTA AACTGGTGGC CTCGGAACAG 
TTGTTTATTT ATGTGAATCA GTCCTTTGCC CCTTCCCCAG ACCAAGAAGT TGGAACTCTA 
TATGAGTGTT TTGGCAGTGA TGGTAAACTG GTCCTGCATT ACTGCAAATC CCAGGCATGG 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg12 ( XM_006526187.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526250.1 Mus musculus autophagy related 12 (Atg12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54745
Clone ID Related Accession (Same CDS sequence)	XM_006526187.2 , XM_006526187.1
Accession Version	XM_006526187.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin-like protein ATG12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526187.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAG ATTCAGAGGT TGTGCTGCAG CTCCCCTCGG CTCCTGTAGG AGCCGGCGGC 
GAGAGCCTTC CAGAGCTCTC CCCGGAAACA GCCACCCCAG AGCCCCCGTC CTCGGCTGCA 
GTTTCGCCCG GAACGGAGGA ACCTCCCGGA GACACCAAGA AAAAAATTTA TTTATGTGAA 
TCAGTCCTTT GCCCCTTCCC CAGACCAAGA AGTTGGAACT CTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526250.1
CDS	179..406
Translation	MSEDSEVVLQLPSAPVGAGGESLPELSPETATPEPPSSAAVSPGTEEPPGDTKKKIYLCESVLCPFPRPRSWNSI

Target ORF information:

RefSeq Version	XM_006526187.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy related 12 (Atg12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526187.2

ORF Insert Sequence:

ATGTCGGAAG ATTCAGAGGT TGTGCTGCAG CTCCCCTCGG CTCCTGTAGG AGCCGGCGGC 
GAGAGCCTTC CAGAGCTCTC CCCGGAAACA GCCACCCCAG AGCCCCCGTC CTCGGCTGCA 
GTTTCGCCCG GAACGGAGGA ACCTCCCGGA GACACCAAGA AAAAAATTTA TTTATGTGAA 
TCAGTCCTTT GCCCCTTCCC CAGACCAAGA AGTTGGAACT CTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Induction of mitophagy in the HEI-OC1 auditory cell line and activation of the Atg12/LC3 pathway in the organ of Corti.
Hearing research(2018 Jan)
Setz C,Benischke AS,Pinho Ferreira Bento AC,Brand Y,Levano S,Paech F,Leitmeyer K,Bodmer D

Mir505-3p regulates axonal development via inhibiting the autophagy pathway by targeting Atg12.
Autophagy13(10)1679-1696(2017 Oct)
Yang K,Yu B,Cheng C,Cheng T,Yuan B,Li K,Xiao J,Qiu Z,Zhou Y

Beige Adipocyte Maintenance Is Regulated by Autophagy-Induced Mitochondrial Clearance.
Cell metabolism24(3)402-419(2016 Sep)
Altshuler-Keylin S,Shinoda K,Hasegawa Y,Ikeda K,Hong H,Kang Q,Yang Y,Perera RM,Debnath J,Kajimura S

miR-30b inhibits autophagy to alleviate hepatic ischemia-reperfusion injury via decreasing the Atg12-Atg5 conjugate.
World journal of gastroenterology22(18)4501-14(2016 May)
Li SP,He JD,Wang Z,Yu Y,Fu SY,Zhang HM,Zhang JJ,Shen ZY

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Atg12 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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