Bbs2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bbs2
Entrez Gene ID	67378
Full Name	Bardet-Biedl syndrome 2 (human)
Synonyms	2410125H22Rik,AI447581
General protein information	Preferred Names Bardet-Biedl syndrome 2 (human) Names Bardet-Biedl syndrome 2 protein homolog Bardet-Biedl syndrome 2 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_026116.3	NP_080392.1	Bardet-Biedl syndrome 2 protein homolog
XM_006531304.3	XP_006531367.1	Bardet-Biedl syndrome 2 protein homolog isoform X2
XM_006531304.2	XP_006531367.1	Bardet-Biedl syndrome 2 protein homolog isoform X1
NM_026116.2	NP_080392.1	Bardet-Biedl syndrome 2 protein homolog
XM_030243735.1	XP_030099595.1	Bardet-Biedl syndrome 2 protein homolog isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BBS2	XP_510980.2
	BBS2	XP_001091861.1
Rattus norvegicus (Norway rat)	Bbs2	NP_446070.1
Danio rerio (zebrafish)	bbs2	NP_690843.1
Homo sapiens (human)	BBS2	NP_114091.3
Bos taurus (cattle)	BBS2	NP_001033249.1
Mus musculus (house mouse)	Bbs2	NP_080392.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009637	XP_318670.4
Canis lupus familiaris (dog)	BBS2	XP_535296.3
Gallus gallus (chicken)	BBS2	NP_001012795.1
Caenorhabditis elegans (roundworm)	bbs-2	NP_501325.1
Xenopus tropicalis (tropical clawed frog)	LOC100493660	XP_004913584.1

Gene Ontology
Bibliography

Related articles in PubMed

DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.
Zhang B, Wang G, Xu X, Yang S, Zhuang T, Wang G, Ren H, Cheng SY, Jiang Q, Zhang C
The Journal of biological chemistry292(4)1351-1360(2017 01)

Regulation of Insulin Receptor Trafficking by Bardet Biedl Syndrome Proteins.
Starks RD, Beyer AM, Guo DF, Boland L, Zhang Q, Sheffield VC, Rahmouni K
PLoS genetics11(6)e1005311(2015 Jun)

Ciliary proteins Bbs8 and Ift20 promote planar cell polarity in the cochlea.
May-Simera HL, Petralia RS, Montcouquiol M, Wang YX, Szarama KB, Liu Y, Lin W, Deans MR, Pazour GJ, Kelley MW
Development (Cambridge, England)142(3)555-66(2015 Feb)

Direct evidence for BBSome-associated intraflagellar transport reveals distinct properties of native mammalian cilia.
Williams CL, McIntyre JC, Norris SR, Jenkins PM, Zhang L, Pei Q, Verhey K, Martens JR
Nature communications55813(2014 Dec)

The centriolar satellite protein AZI1 interacts with BBS4 and regulates ciliary trafficking of the BBSome.
Chamling X, Seo S, Searby CC, Kim G, Slusarski DC, Sheffield VC
PLoS genetics10(2)e1004083(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bbs1, Bbs2, and Bbs4 proteins (BBSome) are bona fide constituents of intraflagellar transport in olfactory sensory neurons.
Title: Direct evidence for BBSome-associated intraflagellar transport reveals distinct properties of native mammalian cilia.

Data implicate Bardet-Biedl syndrome genes in the regulation of vascular function and demonstrate that disrupting Bbs2 and Bbs6 genes affect differentially the vascular function.
Title: Contrasting vascular effects caused by loss of Bardet-Biedl syndrome genes.

Bardet-Biedl syndrome (BBS) proteins mediate LepR trafficking and that impaired LepR signaling underlies energy imbalance in BBS.
Title: Requirement of Bardet-Biedl syndrome proteins for leptin receptor signaling.

a lack of ciliary localization of somatostatin receptor type 3 (Sstr3) and melanin-concentrating hormone receptor 1 (Mchr1) in neurons from mice lacking the Bbs2 or Bbs4 gene
Title: Bardet-Biedl syndrome proteins are required for the localization of G protein-coupled receptors to primary cilia.

Although BBS proteins were not required for ciliogenesis, their loss caused structural defects in a fraction of cilia covering mouse airway epithelia in Bbs1, Bbs2, Bbs4, and Bbs6 mutant mice.
Title: Loss of Bardet-Biedl syndrome proteins alters the morphology and function of motile cilia in airway epithelia.

The following Bbs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bbs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21720	NM_026116.3 Latest version!	Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OMu41691	XM_006531304.3 Latest version!	Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu89930	XM_030243735.1 Latest version!	Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu41691	XM_006531304.2	Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu21720	NM_026116.2	Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bbs2 ( XM_006531304.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531367.1 Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu41691
Clone ID Related Accession (Same CDS sequence)	XM_006531304.3 , XM_006531304.2
Accession Version	XM_006531304.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	Bardet-Biedl syndrome 2 protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006531304.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC CTGTGTTCAC TCTGAAACTG CGCCACAAAA TCAGCCCCCG CATGGTGGCC 
ATAGGGCGCT ACGACGGGAC TCACCCGTGC TTGGCTGCCG CCACCCAAGC GGGCAAGGTT 
TTCATTCATA ACCCTCACAC GCGGAGCCAG CATTTCAGTG CTTCCAGAGT GTTCCAGAGC 
CCTCTGGAGT CTGATGTCTC TCTGCTCAAC ATTAACCAGA CGGTCAGCTG CCTGGGTTCG 
GGAGTCTTGA ACCCTGAGCT TGGCTATGAT ACTCTTTTAG TGGGGACACA GACCAGTCTT 
TTGGCTTACG ACATCTACAA TAATTCAGAT TTGTTCTACA GAGAGGTCTC AGATGGGGCA 
AATGCCATTG TGCTGGGGAC CCTGGGAGAC ATTGCCCCAC CTCTTGCAAT CATCGGTGGA 
AACTGTGCTC TGCAGGGTTT CGATCACGAA GGAAATGATC TCTTTTGGAC GGTTACTGGA 
GACAATGTGC ATTCCTTGGC TCTGTGTGAC TTTGACGGTG ATGGGAAGAC AGAGCTTCTT 
GTTGGATCTG AAGATTTTGA CATTCGAGTT TTTAAAGAAG ATGAGATTGT GGCAGAAATG 
ACAGAGACAG AGATAGTCAC CTCTCTGTGT CCTATGTATG GCAGTCGGTT TGGTTACGCC 
CTTTCTAATG GCACAGTTGG AGTTTATGAC AAAACAGCCC GGTACTGGAG AATTAAATCC 
AAAAACCATG CCATGAGCAT CCATGCTTTT GACATTAATT CTGATGGAGT GTGTGAACTG 
ATAACCGGGT GGTCTAATGG GAAGGTTGAT GCTCGCAGTG ACCGGACTGG AGAGGTCATA 
TTTAAAGACA ACTTCTCTTC TGCTGTTGCT GGTGTGGTAG AGGGAGATTA CCGGATGGAT 
GGCCACGTAC AGTTAATCTG CTGCTCAGTG GATGGGGAAA TCCGAGGCTA CCTGCCAGGC 
ACAGCTGAGA TGAAGGGGAA CCTCCTGGAC ACAAGTGTGG AGCAGGACCT GATCCGAGAG 
CTGAGTCAGA AAAAGCAGAA CCTGTTGCTA GAACTTCGCA ACTATGAGGA GAGCACCAAG 
GCAGAACTGA GCAGTCCCCT GAACGAGGCC GATGGGCAGA AAGGCATAAT CCCAGCCAAT 
ACCAGGCTCC ACACAGCGCT CTCAGTCAAT ATGGGGAATG ACTTACAAGA TGCGCATGCG 
GAATTAGGCA TTTCCACTTC CAATGATACT ATCATCCGAG CAGTACTAAT TTTTGCAGAG 
GGAATTTTTG TGGGTGAAAG CCATGTGGTC CACCCCAGCA TTCACAACCT TTCCAGCTCC 
CTCCGGGTAC CAATTACACC TCCCAAAGAT GTCCCTGTGG ATCTGCACCT GAAAACCTTC 
GTGGGTTACA GAAGCAGCAC CCAGTTCCAT GTGTTTGAAC TGACAAGACA GCTGCCCCGA 
TTCACCATGT ATGCATTGAC AAGCCCAGAT GCAGCTAGCG AGCCTGTCAG CTACGTGAAC 
TTCAGTGTTG CCGAGCGCAC ACAGAGGTTC CTTGCAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531367.1
CDS	1019..2560
Translation	MLLPVFTLKLRHKISPRMVAIGRYDGTHPCLAAATQAGKVFIHNPHTRSQHFSASRVFQSPLESDVSLLNINQTVSCLGSGVLNPELGYDTLLVGTQTSLLAYDIYNNSDLFYREVSDGANAIVLGTLGDIAPPLAIIGGNCALQGFDHEGNDLFWTVTGDNVHSLALCDFDGDGKTELLVGSEDFDIRVFKEDEIVAEMTETEIVTSLCPMYGSRFGYALSNGTVGVYDKTARYWRIKSKNHAMSIHAFDINSDGVCELITGWSNGKVDARSDRTGEVIFKDNFSSAVAGVVEGDYRMDGHVQLICCSVDGEIRGYLPGTAEMKGNLLDTSVEQDLIRELSQKKQNLLLELRNYEESTKAELSSPLNEADGQKGIIPANTRLHTALSVNMGNDLQDAHAELGISTSNDTIIRAVLIFAEGIFVGESHVVHPSIHNLSSSLRVPITPPKDVPVDLHLKTFVGYRSSTQFHVFELTRQLPRFTMYALTSPDAASEPVSYVNFSVAERTQRFLAR

Target ORF information:

RefSeq Version	XM_006531304.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531304.2

ORF Insert Sequence:

ATGCTGCTGC CTGTGTTCAC TCTGAAACTG CGCCACAAAA TCAGCCCCCG CATGGTGGCC 
ATAGGGCGCT ACGACGGGAC TCACCCGTGC TTGGCTGCCG CCACCCAAGC GGGCAAGGTT 
TTCATTCATA ACCCTCACAC GCGGAGCCAG CATTTCAGTG CTTCCAGAGT GTTCCAGAGC 
CCTCTGGAGT CTGATGTCTC TCTGCTCAAC ATTAACCAGA CGGTCAGCTG CCTGGGTTCG 
GGAGTCTTGA ACCCTGAGCT TGGCTATGAT ACTCTTTTAG TGGGGACACA GACCAGTCTT 
TTGGCTTACG ACATCTACAA TAATTCAGAT TTGTTCTACA GAGAGGTCTC AGATGGGGCA 
AATGCCATTG TGCTGGGGAC CCTGGGAGAC ATTGCCCCAC CTCTTGCAAT CATCGGTGGA 
AACTGTGCTC TGCAGGGTTT CGATCACGAA GGAAATGATC TCTTTTGGAC GGTTACTGGA 
GACAATGTGC ATTCCTTGGC TCTGTGTGAC TTTGACGGTG ATGGGAAGAC AGAGCTTCTT 
GTTGGATCTG AAGATTTTGA CATTCGAGTT TTTAAAGAAG ATGAGATTGT GGCAGAAATG 
ACAGAGACAG AGATAGTCAC CTCTCTGTGT CCTATGTATG GCAGTCGGTT TGGTTACGCC 
CTTTCTAATG GCACAGTTGG AGTTTATGAC AAAACAGCCC GGTACTGGAG AATTAAATCC 
AAAAACCATG CCATGAGCAT CCATGCTTTT GACATTAATT CTGATGGAGT GTGTGAACTG 
ATAACCGGGT GGTCTAATGG GAAGGTTGAT GCTCGCAGTG ACCGGACTGG AGAGGTCATA 
TTTAAAGACA ACTTCTCTTC TGCTGTTGCT GGTGTGGTAG AGGGAGATTA CCGGATGGAT 
GGCCACGTAC AGTTAATCTG CTGCTCAGTG GATGGGGAAA TCCGAGGCTA CCTGCCAGGC 
ACAGCTGAGA TGAAGGGGAA CCTCCTGGAC ACAAGTGTGG AGCAGGACCT GATCCGAGAG 
CTGAGTCAGA AAAAGCAGAA CCTGTTGCTA GAACTTCGCA ACTATGAGGA GAGCACCAAG 
GCAGAACTGA GCAGTCCCCT GAACGAGGCC GATGGGCAGA AAGGCATAAT CCCAGCCAAT 
ACCAGGCTCC ACACAGCGCT CTCAGTCAAT ATGGGGAATG ACTTACAAGA TGCGCATGCG 
GAATTAGGCA TTTCCACTTC CAATGATACT ATCATCCGAG CAGTACTAAT TTTTGCAGAG 
GGAATTTTTG TGGGTGAAAG CCATGTGGTC CACCCCAGCA TTCACAACCT TTCCAGCTCC 
CTCCGGGTAC CAATTACACC TCCCAAAGAT GTCCCTGTGG ATCTGCACCT GAAAACCTTC 
GTGGGTTACA GAAGCAGCAC CCAGTTCCAT GTGTTTGAAC TGACAAGACA GCTGCCCCGA 
TTCACCATGT ATGCATTGAC AAGCCCAGAT GCAGCTAGCG AGCCTGTCAG CTACGTGAAC 
TTCAGTGTTG CCGAGCGCAC ACAGAGGTTC CTTGCAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bbs2 ( NM_026116.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080392.1 Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), mRNA.

Price & Availability↑

CloneID	OMu21720
Clone ID Related Accession (Same CDS sequence)	NM_026116.3 , NM_026116.2
Accession Version	NM_026116.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	Bardet-Biedl syndrome 2 protein homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131733.3 and AC138118.4. On Jun 14, 2018 this sequence version replaced NM_026116.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF342737.1, BC057184.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGC CTGTGTTCAC TCTGAAACTG CGCCACAAAA TCAGCCCCCG CATGGTGGCC 
ATAGGGCGCT ACGACGGGAC TCACCCGTGC TTGGCTGCCG CCACCCAAGC GGGCAAGGTT 
TTCATTCATA ACCCTCACAC GCGGAGCCAG CATTTCAGTG CTTCCAGAGT GTTCCAGAGC 
CCTCTGGAGT CTGATGTCTC TCTGCTCAAC ATTAACCAGA CGGTCAGCTG CCTGGGTTCG 
GGAGTCTTGA ACCCTGAGCT TGGCTATGAT ACTCTTTTAG TGGGGACACA GACCAGTCTT 
TTGGCTTACG ACATCTACAA TAATTCAGAT TTGTTCTACA GAGAGGTCTC AGATGGGGCA 
AATGCCATTG TGCTGGGGAC CCTGGGAGAC ATTGCCCCAC CTCTTGCAAT CATCGGTGGA 
AACTGTGCTC TGCAGGGTTT CGATCACGAA GGAAATGATC TCTTTTGGAC GGTTACTGGA 
GACAATGTGC ATTCCTTGGC TCTGTGTGAC TTTGACGGTG ATGGGAAGAC AGAGCTTCTT 
GTTGGATCTG AAGATTTTGA CATTCGAGTT TTTAAAGAAG ATGAGATTGT GGCAGAAATG 
ACAGAGACAG AGATAGTCAC CTCTCTGTGT CCTATGTATG GCAGTCGGTT TGGTTACGCC 
CTTTCTAATG GCACAGTTGG AGTTTATGAC AAAACAGCCC GGTACTGGAG AATTAAATCC 
AAAAACCATG CCATGAGCAT CCATGCTTTT GACATTAATT CTGATGGAGT GTGTGAACTG 
ATAACCGGGT GGTCTAATGG GAAGGTTGAT GCTCGCAGTG ACCGGACTGG AGAGGTCATA 
TTTAAAGACA ACTTCTCTTC TGCTGTTGCT GGTGTGGTAG AGGGAGATTA CCGGATGGAT 
GGCCACGTAC AGTTAATCTG CTGCTCAGTG GATGGGGAAA TCCGAGGCTA CCTGCCAGGC 
ACAGCTGAGA TGAAGGGGAA CCTCCTGGAC ACAAGTGTGG AGCAGGACCT GATCCGAGAG 
CTGAGTCAGA AAAAGCAGAA CCTGTTGCTA GAACTTCGCA ACTATGAGGA GAGCACCAAG 
GCAGAACTGA GCAGTCCCCT GAACGAGGCC GATGGGCAGA AAGGCATAAT CCCAGCCAAT 
ACCAGGCTCC ACACAGCGCT CTCAGTCAAT ATGGGGAATG ACTTACAAGA TGCGCATGCG 
GAATTAGGCA TTTCCACTTC CAATGATACT ATCATCCGAG CAGTACTAAT TTTTGCAGAG 
GGAATTTTTG TGGGTGAAAG CCATGTGGTC CACCCCAGCA TTCACAACCT TTCCAGCTCC 
CTCCGGGTAC CAATTACACC TCCCAAAGAT GTCCCTGTGG ATCTGCACCT GAAAACCTTC 
GTGGGTTACA GAAGCAGCAC CCAGTTCCAT GTGTTTGAAC TGACAAGACA GCTGCCCCGA 
TTCACCATGT ATGCATTGAC AAGCCCAGAT GCAGCTAGCG AGCCTGTCAG CTACGTGAAC 
TTCAGTGTTG CCGAGCGCAC ACAGAGGATG GTCACGTGGC TCAACCAGAA CTTTCTGTTG 
CCAGAAGACA GTAACGTTCA GAATTCCCCG TTTCACGTCT GTTTCACATC GTTACGCAAT 
GGTGGCCAGC TCTACATAAA AATGAAACAA AGTGGTGAGA TCACTGTGAA TACTGACGAT 
ATTGATCTAG CTGGTGACAT CATCCAGTCG ATCGCGTCCT TTTTTGCTAT TGAAGACCTT 
CAGGTAGAAG CAGATTTCCC TGTGTACTTC GAGGAACTAC GGAAAGTGCT GGTGAAGGTG 
GATGAGTATC ATTCAGTGCA TCAAAAGCTC AGCGCCGACA TGGCTGATAA TTCTAACCTC 
ATCCGAAGTT TGCTGGTCAG AGCGGAGGAT GCTCGGCTGA TGAGGGACAT GAAAACAATG 
AAGAGTCGTT ACATGGAGCT CTATGACCTT AATAAAGATT TGCTGAATGG ATACAAGATT 
CGCTGTAACA ACCACACAGA ACTGCTAGGA AACCTGAAGG CGGTGAACCA AGCAATCCAA 
AGAGCAGGCC GTCTGCGCGT TGGAAAGCCA AAGAACCAGG TGATCAGTGC TTGTCGGGAT 
GCGATTCGAA GCAACAACAT CAACACCCTC TTCAGAATCA TGCGAGTGGG CACTGCTCCT 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080392.1
CDS	158..2323
Translation	MLLPVFTLKLRHKISPRMVAIGRYDGTHPCLAAATQAGKVFIHNPHTRSQHFSASRVFQSPLESDVSLLNINQTVSCLGSGVLNPELGYDTLLVGTQTSLLAYDIYNNSDLFYREVSDGANAIVLGTLGDIAPPLAIIGGNCALQGFDHEGNDLFWTVTGDNVHSLALCDFDGDGKTELLVGSEDFDIRVFKEDEIVAEMTETEIVTSLCPMYGSRFGYALSNGTVGVYDKTARYWRIKSKNHAMSIHAFDINSDGVCELITGWSNGKVDARSDRTGEVIFKDNFSSAVAGVVEGDYRMDGHVQLICCSVDGEIRGYLPGTAEMKGNLLDTSVEQDLIRELSQKKQNLLLELRNYEESTKAELSSPLNEADGQKGIIPANTRLHTALSVNMGNDLQDAHAELGISTSNDTIIRAVLIFAEGIFVGESHVVHPSIHNLSSSLRVPITPPKDVPVDLHLKTFVGYRSSTQFHVFELTRQLPRFTMYALTSPDAASEPVSYVNFSVAERTQRMVTWLNQNFLLPEDSNVQNSPFHVCFTSLRNGGQLYIKMKQSGEITVNTDDIDLAGDIIQSIASFFAIEDLQVEADFPVYFEELRKVLVKVDEYHSVHQKLSADMADNSNLIRSLLVRAEDARLMRDMKTMKSRYMELYDLNKDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRVGKPKNQVISACRDAIRSNNINTLFRIMRVGTAPS

Target ORF information:

RefSeq Version	NM_026116.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026116.3

ORF Insert Sequence:

ATGCTGCTGC CTGTGTTCAC TCTGAAACTG CGCCACAAAA TCAGCCCCCG CATGGTGGCC 
ATAGGGCGCT ACGACGGGAC TCACCCGTGC TTGGCTGCCG CCACCCAAGC GGGCAAGGTT 
TTCATTCATA ACCCTCACAC GCGGAGCCAG CATTTCAGTG CTTCCAGAGT GTTCCAGAGC 
CCTCTGGAGT CTGATGTCTC TCTGCTCAAC ATTAACCAGA CGGTCAGCTG CCTGGGTTCG 
GGAGTCTTGA ACCCTGAGCT TGGCTATGAT ACTCTTTTAG TGGGGACACA GACCAGTCTT 
TTGGCTTACG ACATCTACAA TAATTCAGAT TTGTTCTACA GAGAGGTCTC AGATGGGGCA 
AATGCCATTG TGCTGGGGAC CCTGGGAGAC ATTGCCCCAC CTCTTGCAAT CATCGGTGGA 
AACTGTGCTC TGCAGGGTTT CGATCACGAA GGAAATGATC TCTTTTGGAC GGTTACTGGA 
GACAATGTGC ATTCCTTGGC TCTGTGTGAC TTTGACGGTG ATGGGAAGAC AGAGCTTCTT 
GTTGGATCTG AAGATTTTGA CATTCGAGTT TTTAAAGAAG ATGAGATTGT GGCAGAAATG 
ACAGAGACAG AGATAGTCAC CTCTCTGTGT CCTATGTATG GCAGTCGGTT TGGTTACGCC 
CTTTCTAATG GCACAGTTGG AGTTTATGAC AAAACAGCCC GGTACTGGAG AATTAAATCC 
AAAAACCATG CCATGAGCAT CCATGCTTTT GACATTAATT CTGATGGAGT GTGTGAACTG 
ATAACCGGGT GGTCTAATGG GAAGGTTGAT GCTCGCAGTG ACCGGACTGG AGAGGTCATA 
TTTAAAGACA ACTTCTCTTC TGCTGTTGCT GGTGTGGTAG AGGGAGATTA CCGGATGGAT 
GGCCACGTAC AGTTAATCTG CTGCTCAGTG GATGGGGAAA TCCGAGGCTA CCTGCCAGGC 
ACAGCTGAGA TGAAGGGGAA CCTCCTGGAC ACAAGTGTGG AGCAGGACCT GATCCGAGAG 
CTGAGTCAGA AAAAGCAGAA CCTGTTGCTA GAACTTCGCA ACTATGAGGA GAGCACCAAG 
GCAGAACTGA GCAGTCCCCT GAACGAGGCC GATGGGCAGA AAGGCATAAT CCCAGCCAAT 
ACCAGGCTCC ACACAGCGCT CTCAGTCAAT ATGGGGAATG ACTTACAAGA TGCGCATGCG 
GAATTAGGCA TTTCCACTTC CAATGATACT ATCATCCGAG CAGTACTAAT TTTTGCAGAG 
GGAATTTTTG TGGGTGAAAG CCATGTGGTC CACCCCAGCA TTCACAACCT TTCCAGCTCC 
CTCCGGGTAC CAATTACACC TCCCAAAGAT GTCCCTGTGG ATCTGCACCT GAAAACCTTC 
GTGGGTTACA GAAGCAGCAC CCAGTTCCAT GTGTTTGAAC TGACAAGACA GCTGCCCCGA 
TTCACCATGT ATGCATTGAC AAGCCCAGAT GCAGCTAGCG AGCCTGTCAG CTACGTGAAC 
TTCAGTGTTG CCGAGCGCAC ACAGAGGATG GTCACGTGGC TCAACCAGAA CTTTCTGTTG 
CCAGAAGACA GTAACGTTCA GAATTCCCCG TTTCACGTCT GTTTCACATC GTTACGCAAT 
GGTGGCCAGC TCTACATAAA AATGAAACAA AGTGGTGAGA TCACTGTGAA TACTGACGAT 
ATTGATCTAG CTGGTGACAT CATCCAGTCG ATCGCGTCCT TTTTTGCTAT TGAAGACCTT 
CAGGTAGAAG CAGATTTCCC TGTGTACTTC GAGGAACTAC GGAAAGTGCT GGTGAAGGTG 
GATGAGTATC ATTCAGTGCA TCAAAAGCTC AGCGCCGACA TGGCTGATAA TTCTAACCTC 
ATCCGAAGTT TGCTGGTCAG AGCGGAGGAT GCTCGGCTGA TGAGGGACAT GAAAACAATG 
AAGAGTCGTT ACATGGAGCT CTATGACCTT AATAAAGATT TGCTGAATGG ATACAAGATT 
CGCTGTAACA ACCACACAGA ACTGCTAGGA AACCTGAAGG CGGTGAACCA AGCAATCCAA 
AGAGCAGGCC GTCTGCGCGT TGGAAAGCCA AAGAACCAGG TGATCAGTGC TTGTCGGGAT 
GCGATTCGAA GCAACAACAT CAACACCCTC TTCAGAATCA TGCGAGTGGG CACTGCTCCT 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bbs2 ( NM_026116.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080392.1 Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), mRNA.

Price & Availability↑

CloneID	OMu21720
Clone ID Related Accession (Same CDS sequence)	NM_026116.3 , NM_026116.2
Accession Version	NM_026116.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Mus musculus(house mouse)
Product	Bardet-Biedl syndrome 2 protein homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF342737.1. On Jun 19, 2003 this sequence version replaced NM_026116.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF342737.1, BC057184.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCTGC CTGTGTTCAC TCTGAAACTG CGCCACAAAA TCAGCCCCCG CATGGTGGCC 
ATAGGGCGCT ACGACGGGAC TCACCCGTGC TTGGCTGCCG CCACCCAAGC GGGCAAGGTT 
TTCATTCATA ACCCTCACAC GCGGAGCCAG CATTTCAGTG CTTCCAGAGT GTTCCAGAGC 
CCTCTGGAGT CTGATGTCTC TCTGCTCAAC ATTAACCAGA CGGTCAGCTG CCTGGGTTCG 
GGAGTCTTGA ACCCTGAGCT TGGCTATGAT ACTCTTTTAG TGGGGACACA GACCAGTCTT 
TTGGCTTACG ACATCTACAA TAATTCAGAT TTGTTCTACA GAGAGGTCTC AGATGGGGCA 
AATGCCATTG TGCTGGGGAC CCTGGGAGAC ATTGCCCCAC CTCTTGCAAT CATCGGTGGA 
AACTGTGCTC TGCAGGGTTT CGATCACGAA GGAAATGATC TCTTTTGGAC GGTTACTGGA 
GACAATGTGC ATTCCTTGGC TCTGTGTGAC TTTGACGGTG ATGGGAAGAC AGAGCTTCTT 
GTTGGATCTG AAGATTTTGA CATTCGAGTT TTTAAAGAAG ATGAGATTGT GGCAGAAATG 
ACAGAGACAG AGATAGTCAC CTCTCTGTGT CCTATGTATG GCAGTCGGTT TGGTTACGCC 
CTTTCTAATG GCACAGTTGG AGTTTATGAC AAAACAGCCC GGTACTGGAG AATTAAATCC 
AAAAACCATG CCATGAGCAT CCATGCTTTT GACATTAATT CTGATGGAGT GTGTGAACTG 
ATAACCGGGT GGTCTAATGG GAAGGTTGAT GCTCGCAGTG ACCGGACTGG AGAGGTCATA 
TTTAAAGACA ACTTCTCTTC TGCTGTTGCT GGTGTGGTAG AGGGAGATTA CCGGATGGAT 
GGCCACGTAC AGTTAATCTG CTGCTCAGTG GATGGGGAAA TCCGAGGCTA CCTGCCAGGC 
ACAGCTGAGA TGAAGGGGAA CCTCCTGGAC ACAAGTGTGG AGCAGGACCT GATCCGAGAG 
CTGAGTCAGA AAAAGCAGAA CCTGTTGCTA GAACTTCGCA ACTATGAGGA GAGCACCAAG 
GCAGAACTGA GCAGTCCCCT GAACGAGGCC GATGGGCAGA AAGGCATAAT CCCAGCCAAT 
ACCAGGCTCC ACACAGCGCT CTCAGTCAAT ATGGGGAATG ACTTACAAGA TGCGCATGCG 
GAATTAGGCA TTTCCACTTC CAATGATACT ATCATCCGAG CAGTACTAAT TTTTGCAGAG 
GGAATTTTTG TGGGTGAAAG CCATGTGGTC CACCCCAGCA TTCACAACCT TTCCAGCTCC 
CTCCGGGTAC CAATTACACC TCCCAAAGAT GTCCCTGTGG ATCTGCACCT GAAAACCTTC 
GTGGGTTACA GAAGCAGCAC CCAGTTCCAT GTGTTTGAAC TGACAAGACA GCTGCCCCGA 
TTCACCATGT ATGCATTGAC AAGCCCAGAT GCAGCTAGCG AGCCTGTCAG CTACGTGAAC 
TTCAGTGTTG CCGAGCGCAC ACAGAGGATG GTCACGTGGC TCAACCAGAA CTTTCTGTTG 
CCAGAAGACA GTAACGTTCA GAATTCCCCG TTTCACGTCT GTTTCACATC GTTACGCAAT 
GGTGGCCAGC TCTACATAAA AATGAAACAA AGTGGTGAGA TCACTGTGAA TACTGACGAT 
ATTGATCTAG CTGGTGACAT CATCCAGTCG ATCGCGTCCT TTTTTGCTAT TGAAGACCTT 
CAGGTAGAAG CAGATTTCCC TGTGTACTTC GAGGAACTAC GGAAAGTGCT GGTGAAGGTG 
GATGAGTATC ATTCAGTGCA TCAAAAGCTC AGCGCCGACA TGGCTGATAA TTCTAACCTC 
ATCCGAAGTT TGCTGGTCAG AGCGGAGGAT GCTCGGCTGA TGAGGGACAT GAAAACAATG 
AAGAGTCGTT ACATGGAGCT CTATGACCTT AATAAAGATT TGCTGAATGG ATACAAGATT 
CGCTGTAACA ACCACACAGA ACTGCTAGGA AACCTGAAGG CGGTGAACCA AGCAATCCAA 
AGAGCAGGCC GTCTGCGCGT TGGAAAGCCA AAGAACCAGG TGATCAGTGC TTGTCGGGAT 
GCGATTCGAA GCAACAACAT CAACACCCTC TTCAGAATCA TGCGAGTGGG CACTGCTCCT 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080392.1
CDS	460..2625
Translation	MLLPVFTLKLRHKISPRMVAIGRYDGTHPCLAAATQAGKVFIHNPHTRSQHFSASRVFQSPLESDVSLLNINQTVSCLGSGVLNPELGYDTLLVGTQTSLLAYDIYNNSDLFYREVSDGANAIVLGTLGDIAPPLAIIGGNCALQGFDHEGNDLFWTVTGDNVHSLALCDFDGDGKTELLVGSEDFDIRVFKEDEIVAEMTETEIVTSLCPMYGSRFGYALSNGTVGVYDKTARYWRIKSKNHAMSIHAFDINSDGVCELITGWSNGKVDARSDRTGEVIFKDNFSSAVAGVVEGDYRMDGHVQLICCSVDGEIRGYLPGTAEMKGNLLDTSVEQDLIRELSQKKQNLLLELRNYEESTKAELSSPLNEADGQKGIIPANTRLHTALSVNMGNDLQDAHAELGISTSNDTIIRAVLIFAEGIFVGESHVVHPSIHNLSSSLRVPITPPKDVPVDLHLKTFVGYRSSTQFHVFELTRQLPRFTMYALTSPDAASEPVSYVNFSVAERTQRMVTWLNQNFLLPEDSNVQNSPFHVCFTSLRNGGQLYIKMKQSGEITVNTDDIDLAGDIIQSIASFFAIEDLQVEADFPVYFEELRKVLVKVDEYHSVHQKLSADMADNSNLIRSLLVRAEDARLMRDMKTMKSRYMELYDLNKDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRVGKPKNQVISACRDAIRSNNINTLFRIMRVGTAPS

Target ORF information:

RefSeq Version	NM_026116.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026116.2

ORF Insert Sequence:

ATGCTGCTGC CTGTGTTCAC TCTGAAACTG CGCCACAAAA TCAGCCCCCG CATGGTGGCC 
ATAGGGCGCT ACGACGGGAC TCACCCGTGC TTGGCTGCCG CCACCCAAGC GGGCAAGGTT 
TTCATTCATA ACCCTCACAC GCGGAGCCAG CATTTCAGTG CTTCCAGAGT GTTCCAGAGC 
CCTCTGGAGT CTGATGTCTC TCTGCTCAAC ATTAACCAGA CGGTCAGCTG CCTGGGTTCG 
GGAGTCTTGA ACCCTGAGCT TGGCTATGAT ACTCTTTTAG TGGGGACACA GACCAGTCTT 
TTGGCTTACG ACATCTACAA TAATTCAGAT TTGTTCTACA GAGAGGTCTC AGATGGGGCA 
AATGCCATTG TGCTGGGGAC CCTGGGAGAC ATTGCCCCAC CTCTTGCAAT CATCGGTGGA 
AACTGTGCTC TGCAGGGTTT CGATCACGAA GGAAATGATC TCTTTTGGAC GGTTACTGGA 
GACAATGTGC ATTCCTTGGC TCTGTGTGAC TTTGACGGTG ATGGGAAGAC AGAGCTTCTT 
GTTGGATCTG AAGATTTTGA CATTCGAGTT TTTAAAGAAG ATGAGATTGT GGCAGAAATG 
ACAGAGACAG AGATAGTCAC CTCTCTGTGT CCTATGTATG GCAGTCGGTT TGGTTACGCC 
CTTTCTAATG GCACAGTTGG AGTTTATGAC AAAACAGCCC GGTACTGGAG AATTAAATCC 
AAAAACCATG CCATGAGCAT CCATGCTTTT GACATTAATT CTGATGGAGT GTGTGAACTG 
ATAACCGGGT GGTCTAATGG GAAGGTTGAT GCTCGCAGTG ACCGGACTGG AGAGGTCATA 
TTTAAAGACA ACTTCTCTTC TGCTGTTGCT GGTGTGGTAG AGGGAGATTA CCGGATGGAT 
GGCCACGTAC AGTTAATCTG CTGCTCAGTG GATGGGGAAA TCCGAGGCTA CCTGCCAGGC 
ACAGCTGAGA TGAAGGGGAA CCTCCTGGAC ACAAGTGTGG AGCAGGACCT GATCCGAGAG 
CTGAGTCAGA AAAAGCAGAA CCTGTTGCTA GAACTTCGCA ACTATGAGGA GAGCACCAAG 
GCAGAACTGA GCAGTCCCCT GAACGAGGCC GATGGGCAGA AAGGCATAAT CCCAGCCAAT 
ACCAGGCTCC ACACAGCGCT CTCAGTCAAT ATGGGGAATG ACTTACAAGA TGCGCATGCG 
GAATTAGGCA TTTCCACTTC CAATGATACT ATCATCCGAG CAGTACTAAT TTTTGCAGAG 
GGAATTTTTG TGGGTGAAAG CCATGTGGTC CACCCCAGCA TTCACAACCT TTCCAGCTCC 
CTCCGGGTAC CAATTACACC TCCCAAAGAT GTCCCTGTGG ATCTGCACCT GAAAACCTTC 
GTGGGTTACA GAAGCAGCAC CCAGTTCCAT GTGTTTGAAC TGACAAGACA GCTGCCCCGA 
TTCACCATGT ATGCATTGAC AAGCCCAGAT GCAGCTAGCG AGCCTGTCAG CTACGTGAAC 
TTCAGTGTTG CCGAGCGCAC ACAGAGGATG GTCACGTGGC TCAACCAGAA CTTTCTGTTG 
CCAGAAGACA GTAACGTTCA GAATTCCCCG TTTCACGTCT GTTTCACATC GTTACGCAAT 
GGTGGCCAGC TCTACATAAA AATGAAACAA AGTGGTGAGA TCACTGTGAA TACTGACGAT 
ATTGATCTAG CTGGTGACAT CATCCAGTCG ATCGCGTCCT TTTTTGCTAT TGAAGACCTT 
CAGGTAGAAG CAGATTTCCC TGTGTACTTC GAGGAACTAC GGAAAGTGCT GGTGAAGGTG 
GATGAGTATC ATTCAGTGCA TCAAAAGCTC AGCGCCGACA TGGCTGATAA TTCTAACCTC 
ATCCGAAGTT TGCTGGTCAG AGCGGAGGAT GCTCGGCTGA TGAGGGACAT GAAAACAATG 
AAGAGTCGTT ACATGGAGCT CTATGACCTT AATAAAGATT TGCTGAATGG ATACAAGATT 
CGCTGTAACA ACCACACAGA ACTGCTAGGA AACCTGAAGG CGGTGAACCA AGCAATCCAA 
AGAGCAGGCC GTCTGCGCGT TGGAAAGCCA AAGAACCAGG TGATCAGTGC TTGTCGGGAT 
GCGATTCGAA GCAACAACAT CAACACCCTC TTCAGAATCA TGCGAGTGGG CACTGCTCCT 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bbs2 ( XM_006531304.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531367.1 Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), transcript variant X3, mRNA.

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CloneID	OMu41691
Clone ID Related Accession (Same CDS sequence)	XM_006531304.3 , XM_006531304.2
Accession Version	XM_006531304.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	Bardet-Biedl syndrome 2 protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531304.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC CTGTGTTCAC TCTGAAACTG CGCCACAAAA TCAGCCCCCG CATGGTGGCC 
ATAGGGCGCT ACGACGGGAC TCACCCGTGC TTGGCTGCCG CCACCCAAGC GGGCAAGGTT 
TTCATTCATA ACCCTCACAC GCGGAGCCAG CATTTCAGTG CTTCCAGAGT GTTCCAGAGC 
CCTCTGGAGT CTGATGTCTC TCTGCTCAAC ATTAACCAGA CGGTCAGCTG CCTGGGTTCG 
GGAGTCTTGA ACCCTGAGCT TGGCTATGAT ACTCTTTTAG TGGGGACACA GACCAGTCTT 
TTGGCTTACG ACATCTACAA TAATTCAGAT TTGTTCTACA GAGAGGTCTC AGATGGGGCA 
AATGCCATTG TGCTGGGGAC CCTGGGAGAC ATTGCCCCAC CTCTTGCAAT CATCGGTGGA 
AACTGTGCTC TGCAGGGTTT CGATCACGAA GGAAATGATC TCTTTTGGAC GGTTACTGGA 
GACAATGTGC ATTCCTTGGC TCTGTGTGAC TTTGACGGTG ATGGGAAGAC AGAGCTTCTT 
GTTGGATCTG AAGATTTTGA CATTCGAGTT TTTAAAGAAG ATGAGATTGT GGCAGAAATG 
ACAGAGACAG AGATAGTCAC CTCTCTGTGT CCTATGTATG GCAGTCGGTT TGGTTACGCC 
CTTTCTAATG GCACAGTTGG AGTTTATGAC AAAACAGCCC GGTACTGGAG AATTAAATCC 
AAAAACCATG CCATGAGCAT CCATGCTTTT GACATTAATT CTGATGGAGT GTGTGAACTG 
ATAACCGGGT GGTCTAATGG GAAGGTTGAT GCTCGCAGTG ACCGGACTGG AGAGGTCATA 
TTTAAAGACA ACTTCTCTTC TGCTGTTGCT GGTGTGGTAG AGGGAGATTA CCGGATGGAT 
GGCCACGTAC AGTTAATCTG CTGCTCAGTG GATGGGGAAA TCCGAGGCTA CCTGCCAGGC 
ACAGCTGAGA TGAAGGGGAA CCTCCTGGAC ACAAGTGTGG AGCAGGACCT GATCCGAGAG 
CTGAGTCAGA AAAAGCAGAA CCTGTTGCTA GAACTTCGCA ACTATGAGGA GAGCACCAAG 
GCAGAACTGA GCAGTCCCCT GAACGAGGCC GATGGGCAGA AAGGCATAAT CCCAGCCAAT 
ACCAGGCTCC ACACAGCGCT CTCAGTCAAT ATGGGGAATG ACTTACAAGA TGCGCATGCG 
GAATTAGGCA TTTCCACTTC CAATGATACT ATCATCCGAG CAGTACTAAT TTTTGCAGAG 
GGAATTTTTG TGGGTGAAAG CCATGTGGTC CACCCCAGCA TTCACAACCT TTCCAGCTCC 
CTCCGGGTAC CAATTACACC TCCCAAAGAT GTCCCTGTGG ATCTGCACCT GAAAACCTTC 
GTGGGTTACA GAAGCAGCAC CCAGTTCCAT GTGTTTGAAC TGACAAGACA GCTGCCCCGA 
TTCACCATGT ATGCATTGAC AAGCCCAGAT GCAGCTAGCG AGCCTGTCAG CTACGTGAAC 
TTCAGTGTTG CCGAGCGCAC ACAGAGGTTC CTTGCAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531367.1
CDS	1018..2559
Translation	MLLPVFTLKLRHKISPRMVAIGRYDGTHPCLAAATQAGKVFIHNPHTRSQHFSASRVFQSPLESDVSLLNINQTVSCLGSGVLNPELGYDTLLVGTQTSLLAYDIYNNSDLFYREVSDGANAIVLGTLGDIAPPLAIIGGNCALQGFDHEGNDLFWTVTGDNVHSLALCDFDGDGKTELLVGSEDFDIRVFKEDEIVAEMTETEIVTSLCPMYGSRFGYALSNGTVGVYDKTARYWRIKSKNHAMSIHAFDINSDGVCELITGWSNGKVDARSDRTGEVIFKDNFSSAVAGVVEGDYRMDGHVQLICCSVDGEIRGYLPGTAEMKGNLLDTSVEQDLIRELSQKKQNLLLELRNYEESTKAELSSPLNEADGQKGIIPANTRLHTALSVNMGNDLQDAHAELGISTSNDTIIRAVLIFAEGIFVGESHVVHPSIHNLSSSLRVPITPPKDVPVDLHLKTFVGYRSSTQFHVFELTRQLPRFTMYALTSPDAASEPVSYVNFSVAERTQRFLAR

Target ORF information:

RefSeq Version	XM_006531304.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531304.3

ORF Insert Sequence:

ATGCTGCTGC CTGTGTTCAC TCTGAAACTG CGCCACAAAA TCAGCCCCCG CATGGTGGCC 
ATAGGGCGCT ACGACGGGAC TCACCCGTGC TTGGCTGCCG CCACCCAAGC GGGCAAGGTT 
TTCATTCATA ACCCTCACAC GCGGAGCCAG CATTTCAGTG CTTCCAGAGT GTTCCAGAGC 
CCTCTGGAGT CTGATGTCTC TCTGCTCAAC ATTAACCAGA CGGTCAGCTG CCTGGGTTCG 
GGAGTCTTGA ACCCTGAGCT TGGCTATGAT ACTCTTTTAG TGGGGACACA GACCAGTCTT 
TTGGCTTACG ACATCTACAA TAATTCAGAT TTGTTCTACA GAGAGGTCTC AGATGGGGCA 
AATGCCATTG TGCTGGGGAC CCTGGGAGAC ATTGCCCCAC CTCTTGCAAT CATCGGTGGA 
AACTGTGCTC TGCAGGGTTT CGATCACGAA GGAAATGATC TCTTTTGGAC GGTTACTGGA 
GACAATGTGC ATTCCTTGGC TCTGTGTGAC TTTGACGGTG ATGGGAAGAC AGAGCTTCTT 
GTTGGATCTG AAGATTTTGA CATTCGAGTT TTTAAAGAAG ATGAGATTGT GGCAGAAATG 
ACAGAGACAG AGATAGTCAC CTCTCTGTGT CCTATGTATG GCAGTCGGTT TGGTTACGCC 
CTTTCTAATG GCACAGTTGG AGTTTATGAC AAAACAGCCC GGTACTGGAG AATTAAATCC 
AAAAACCATG CCATGAGCAT CCATGCTTTT GACATTAATT CTGATGGAGT GTGTGAACTG 
ATAACCGGGT GGTCTAATGG GAAGGTTGAT GCTCGCAGTG ACCGGACTGG AGAGGTCATA 
TTTAAAGACA ACTTCTCTTC TGCTGTTGCT GGTGTGGTAG AGGGAGATTA CCGGATGGAT 
GGCCACGTAC AGTTAATCTG CTGCTCAGTG GATGGGGAAA TCCGAGGCTA CCTGCCAGGC 
ACAGCTGAGA TGAAGGGGAA CCTCCTGGAC ACAAGTGTGG AGCAGGACCT GATCCGAGAG 
CTGAGTCAGA AAAAGCAGAA CCTGTTGCTA GAACTTCGCA ACTATGAGGA GAGCACCAAG 
GCAGAACTGA GCAGTCCCCT GAACGAGGCC GATGGGCAGA AAGGCATAAT CCCAGCCAAT 
ACCAGGCTCC ACACAGCGCT CTCAGTCAAT ATGGGGAATG ACTTACAAGA TGCGCATGCG 
GAATTAGGCA TTTCCACTTC CAATGATACT ATCATCCGAG CAGTACTAAT TTTTGCAGAG 
GGAATTTTTG TGGGTGAAAG CCATGTGGTC CACCCCAGCA TTCACAACCT TTCCAGCTCC 
CTCCGGGTAC CAATTACACC TCCCAAAGAT GTCCCTGTGG ATCTGCACCT GAAAACCTTC 
GTGGGTTACA GAAGCAGCAC CCAGTTCCAT GTGTTTGAAC TGACAAGACA GCTGCCCCGA 
TTCACCATGT ATGCATTGAC AAGCCCAGAT GCAGCTAGCG AGCCTGTCAG CTACGTGAAC 
TTCAGTGTTG CCGAGCGCAC ACAGAGGTTC CTTGCAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bbs2 ( XM_030243735.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099595.1 Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), transcript variant X2, mRNA.

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CloneID	OMu89930
Clone ID Related Accession (Same CDS sequence)	XM_030243735.1
Accession Version	XM_030243735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	Bardet-Biedl syndrome 2 protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGA CAGAGATAGT CACCTCTCTG TGTCCTATGT ATGGCAGTCG GTTTGGTTAC 
GCCCTTTCTA ATGGCACAGT TGGAGTTTAT GACAAAACAG CCCGGTACTG GAGAATTAAA 
TCCAAAAACC ATGCCATGAG CATCCATGCT TTTGACATTA ATTCTGATGG AGTGTGTGAA 
CTGATAACCG GGTGGTCTAA TGGGAAGGTT GATGCTCGCA GTGACCGGAC TGGAGAGGTC 
ATATTTAAAG ACAACTTCTC TTCTGCTGTT GCTGGTGTGG TAGAGGGAGA TTACCGGATG 
GATGGCCACG TACAGTTAAT CTGCTGCTCA GTGGATGGGG AAATCCGAGG CTACCTGCCA 
GGCACAGCTG AGATGAAGGG GAACCTCCTG GACACAAGTG TGGAGCAGGA CCTGATCCGA 
GAGCTGAGTC AGAAAAAGCA GAACCTGTTG CTAGAACTTC GCAACTATGA GGAGAGCACC 
AAGGCAGAAC TGAGCAGTCC CCTGAACGAG GCCGATGGGC AGAAAGGCAT AATCCCAGCC 
AATACCAGGC TCCACACAGC GCTCTCAGTC AATATGGGGA ATGACTTACA AGATGCGCAT 
GCGGAATTAG GCATTTCCAC TTCCAATGAT ACTATCATCC GAGCAGTACT AATTTTTGCA 
GAGGGAATTT TTGTGGGTGA AAGCCATGTG GTCCACCCCA GCATTCACAA CCTTTCCAGC 
TCCCTCCGGG TACCAATTAC ACCTCCCAAA GATGTCCCTG TGGATCTGCA CCTGAAAACC 
TTCGTGGGTT ACAGAAGCAG CACCCAGTTC CATGTGTTTG AACTGACAAG ACAGCTGCCC 
CGATTCACCA TGTATGCATT GACAAGCCCA GATGCAGCTA GCGAGCCTGT CAGCTACGTG 
AACTTCAGTG TTGCCGAGCG CACACAGAGG ATGGTCACGT GGCTCAACCA GAACTTTCTG 
TTGCCAGAAG ACAGTAACGT TCAGAATTCC CCGTTTCACG TCTGTTTCAC ATCGTTACGC 
AATGGTGGCC AGCTCTACAT AAAAATGAAA CAAAGTGGTG AGATCACTGT GAATACTGAC 
GATATTGATC TAGCTGGTGA CATCATCCAG TCGATCGCGT CCTTTTTTGC TATTGAAGAC 
CTTCAGGTAG AAGCAGATTT CCCTGTGTAC TTCGAGGAAC TACGGAAAGT GCTGGTGAAG 
GTGGATGAGT ATCATTCAGT GCATCAAAAG CTCAGCGCCG ACATGGCTGA TAATTCTAAC 
CTCATCCGAA GTTTGCTGGT CAGAGCGGAG GATGCTCGGC TGATGAGGGA CATGAAAACA 
ATGAAGAGTC GTTACATGGA GCTCTATGAC CTTAATAAAG ATTTGCTGAA TGGATACAAG 
ATTCGCTGTA ACAACCACAC AGAACTGCTA GGAAACCTGA AGGCGGTGAA CCAAGCAATC 
CAAAGAGCAG GCCGTCTGCG CGTTGGAAAG CCAAAGAACC AGGTGATCAG TGCTTGTCGG 
GATGCGATTC GAAGCAACAA CATCAACACC CTCTTCAGAA TCATGCGAGT GGGCACTGCT 
CCTTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099595.1
CDS	613..2181
Translation	MTETEIVTSLCPMYGSRFGYALSNGTVGVYDKTARYWRIKSKNHAMSIHAFDINSDGVCELITGWSNGKVDARSDRTGEVIFKDNFSSAVAGVVEGDYRMDGHVQLICCSVDGEIRGYLPGTAEMKGNLLDTSVEQDLIRELSQKKQNLLLELRNYEESTKAELSSPLNEADGQKGIIPANTRLHTALSVNMGNDLQDAHAELGISTSNDTIIRAVLIFAEGIFVGESHVVHPSIHNLSSSLRVPITPPKDVPVDLHLKTFVGYRSSTQFHVFELTRQLPRFTMYALTSPDAASEPVSYVNFSVAERTQRMVTWLNQNFLLPEDSNVQNSPFHVCFTSLRNGGQLYIKMKQSGEITVNTDDIDLAGDIIQSIASFFAIEDLQVEADFPVYFEELRKVLVKVDEYHSVHQKLSADMADNSNLIRSLLVRAEDARLMRDMKTMKSRYMELYDLNKDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRVGKPKNQVISACRDAIRSNNINTLFRIMRVGTAPS

Target ORF information:

RefSeq Version	XM_030243735.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Bardet-Biedl syndrome 2 (human) (Bbs2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243735.1

ORF Insert Sequence:

ATGACAGAGA CAGAGATAGT CACCTCTCTG TGTCCTATGT ATGGCAGTCG GTTTGGTTAC 
GCCCTTTCTA ATGGCACAGT TGGAGTTTAT GACAAAACAG CCCGGTACTG GAGAATTAAA 
TCCAAAAACC ATGCCATGAG CATCCATGCT TTTGACATTA ATTCTGATGG AGTGTGTGAA 
CTGATAACCG GGTGGTCTAA TGGGAAGGTT GATGCTCGCA GTGACCGGAC TGGAGAGGTC 
ATATTTAAAG ACAACTTCTC TTCTGCTGTT GCTGGTGTGG TAGAGGGAGA TTACCGGATG 
GATGGCCACG TACAGTTAAT CTGCTGCTCA GTGGATGGGG AAATCCGAGG CTACCTGCCA 
GGCACAGCTG AGATGAAGGG GAACCTCCTG GACACAAGTG TGGAGCAGGA CCTGATCCGA 
GAGCTGAGTC AGAAAAAGCA GAACCTGTTG CTAGAACTTC GCAACTATGA GGAGAGCACC 
AAGGCAGAAC TGAGCAGTCC CCTGAACGAG GCCGATGGGC AGAAAGGCAT AATCCCAGCC 
AATACCAGGC TCCACACAGC GCTCTCAGTC AATATGGGGA ATGACTTACA AGATGCGCAT 
GCGGAATTAG GCATTTCCAC TTCCAATGAT ACTATCATCC GAGCAGTACT AATTTTTGCA 
GAGGGAATTT TTGTGGGTGA AAGCCATGTG GTCCACCCCA GCATTCACAA CCTTTCCAGC 
TCCCTCCGGG TACCAATTAC ACCTCCCAAA GATGTCCCTG TGGATCTGCA CCTGAAAACC 
TTCGTGGGTT ACAGAAGCAG CACCCAGTTC CATGTGTTTG AACTGACAAG ACAGCTGCCC 
CGATTCACCA TGTATGCATT GACAAGCCCA GATGCAGCTA GCGAGCCTGT CAGCTACGTG 
AACTTCAGTG TTGCCGAGCG CACACAGAGG ATGGTCACGT GGCTCAACCA GAACTTTCTG 
TTGCCAGAAG ACAGTAACGT TCAGAATTCC CCGTTTCACG TCTGTTTCAC ATCGTTACGC 
AATGGTGGCC AGCTCTACAT AAAAATGAAA CAAAGTGGTG AGATCACTGT GAATACTGAC 
GATATTGATC TAGCTGGTGA CATCATCCAG TCGATCGCGT CCTTTTTTGC TATTGAAGAC 
CTTCAGGTAG AAGCAGATTT CCCTGTGTAC TTCGAGGAAC TACGGAAAGT GCTGGTGAAG 
GTGGATGAGT ATCATTCAGT GCATCAAAAG CTCAGCGCCG ACATGGCTGA TAATTCTAAC 
CTCATCCGAA GTTTGCTGGT CAGAGCGGAG GATGCTCGGC TGATGAGGGA CATGAAAACA 
ATGAAGAGTC GTTACATGGA GCTCTATGAC CTTAATAAAG ATTTGCTGAA TGGATACAAG 
ATTCGCTGTA ACAACCACAC AGAACTGCTA GGAAACCTGA AGGCGGTGAA CCAAGCAATC 
CAAAGAGCAG GCCGTCTGCG CGTTGGAAAG CCAAAGAACC AGGTGATCAG TGCTTGTCGG 
GATGCGATTC GAAGCAACAA CATCAACACC CTCTTCAGAA TCATGCGAGT GGGCACTGCT 
CCTTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.
The Journal of biological chemistry292(4)1351-1360(2017 01)
Zhang B,Wang G,Xu X,Yang S,Zhuang T,Wang G,Ren H,Cheng SY,Jiang Q,Zhang C

Regulation of Insulin Receptor Trafficking by Bardet Biedl Syndrome Proteins.
PLoS genetics11(6)e1005311(2015 Jun)
Starks RD,Beyer AM,Guo DF,Boland L,Zhang Q,Sheffield VC,Rahmouni K

Ciliary proteins Bbs8 and Ift20 promote planar cell polarity in the cochlea.
Development (Cambridge, England)142(3)555-66(2015 Feb)
May-Simera HL,Petralia RS,Montcouquiol M,Wang YX,Szarama KB,Liu Y,Lin W,Deans MR,Pazour GJ,Kelley MW

Direct evidence for BBSome-associated intraflagellar transport reveals distinct properties of native mammalian cilia.
Nature communications55813(2014 Dec)
Williams CL,McIntyre JC,Norris SR,Jenkins PM,Zhang L,Pei Q,Verhey K,Martens JR

The centriolar satellite protein AZI1 interacts with BBS4 and regulates ciliary trafficking of the BBSome.
PLoS genetics10(2)e1004083(2014 Feb)
Chamling X,Seo S,Searby CC,Kim G,Slusarski DC,Sheffield VC

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Bbs2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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