bbs2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	bbs2
Entrez Gene ID	100493660
Full Name	Bardet-Biedl syndrome 2
General protein information	Preferred Names Bardet-Biedl syndrome 2 Names Bardet-Biedl syndrome 2 protein Bardet-Biedl syndrome 2 protein homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004913527.4	XP_004913584.1	Bardet-Biedl syndrome 2 protein isoform X1
XM_004913527.3	XP_004913584.1	Bardet-Biedl syndrome 2 protein isoform X1
XM_018093490.1	XP_017948979.1	Bardet-Biedl syndrome 2 protein isoform X5
XM_002937164.4	XP_002937210.2	Bardet-Biedl syndrome 2 protein isoform X4
XM_002937164.5	XP_002937210.2	Bardet-Biedl syndrome 2 protein isoform X3
XM_012962241.2	XP_012817695.1	Bardet-Biedl syndrome 2 protein isoform X3
XM_004913528.4	XP_004913585.1	Bardet-Biedl syndrome 2 protein isoform X2
XM_004913528.3	XP_004913585.1	Bardet-Biedl syndrome 2 protein isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BBS2	XP_510980.2
	BBS2	XP_001091861.1
Rattus norvegicus (Norway rat)	Bbs2	NP_446070.1
Danio rerio (zebrafish)	bbs2	NP_690843.1
Homo sapiens (human)	BBS2	NP_114091.3
Bos taurus (cattle)	BBS2	NP_001033249.1
Mus musculus (house mouse)	Bbs2	NP_080392.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009637	XP_318670.4
Canis lupus familiaris (dog)	BBS2	XP_535296.3
Gallus gallus (chicken)	BBS2	NP_001012795.1
Caenorhabditis elegans (roundworm)	bbs-2	NP_501325.1
Xenopus tropicalis (tropical clawed frog)	LOC100493660	XP_004913584.1

Gene Ontology

The following bbs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bbs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa35746	XM_012962241.2 Latest version!	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa08758	XM_004913527.4 Latest version!	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa08759	XM_004913528.4 Latest version!	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa08760	XM_002937164.5 Latest version!	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa44118	XM_018093490.1 Latest version!	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OXa08758	XM_004913527.3	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa08760	XM_002937164.4	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa08759	XM_004913528.3	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bbs2 ( XM_004913527.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913584.1 Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa08758
Clone ID Related Accession (Same CDS sequence)	XM_004913527.4 , XM_004913527.3
Accession Version	XM_004913527.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Bardet-Biedl syndrome 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913527.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAACTTCTTG TAGGATCTGA AGACTTTGAC 
ATCAGAGTGT TTAAAGAGGA TGAAATTGTG GCTGAAATGT CAGAAACTGA GACTATTACA 
GCACTGTGTC CTATGTATGG CAGTCGCTTT GGATATGCCC TCTCCAATGG AACTGTGGGA 
GTGTATGACC GCACAGCTCG GTACTGGAGA ATAAAATCAA AAAATCTTGC AATGAGTATC 
CATGCATTTG ACCTAAACTC TGATGGAGTG TGCGAGCTGA TCACTGGGTG GTCCAATGGA 
AAGATTGATG CCCGTAGTGA TCGCACTGGG GAGGTGATCT TTAAAGATAA CTTCTGCTCC 
TCTGTAGCTG GAGTGGTGGA AGGTGATTAC CGTATGGATG GCAAGACCCA GCTTATCTGC 
TGTTCTATAG ATGGGGAGGT GCGAGGTTAT CTTCCTACCA GTCAGGAGGC CGCAGGAAGT 
CTAATGGATA CAAGCGTGGA ACAGGAACTG GTGAGAGAGC TCAGTCAGAA GAAGCAGAAT 
CTGCTGTTGG AGCTGAAAAA TTATCAAGAA AATTCAAAGC AGGCAGAGTC CCGGATTTCA 
CAGAGTGAGA CTGATGGGCA GATGGGAGTA ATACCAGCAA ACACACAGCT GCAGACTGCT 
CTTTCTGTGT CCCTGGGCTC TGAAACCCAG AATGCACATG TAGAACTAAG CATTTCAACA 
TCAAATGATA CAATCATTCG GGCAGTGCTG ATCTTTGCAG AAGGGATCTT TGAAGGAGAA 
AGTCATGTTG TTCACCCTAG CACTCAGCAT CTAAGTGGCC TGGTTCGTGT TCCGATTAGC 
CCCCCTAAAG ATGTACCAGT GGATCTGCAC ATCAAAGCAT TTGTGGGTTA CAAGAGCAGT 
GTACAGTTCC ATGTATTTGA GCTGACACGG CAGCTTCCTC GCTTTTCCAT GTATGTGCTA 
AGTAATCCTT CCTCAGCACC AGAGACATCC AGTTATGTAA CATTCACCAC CAGTGAGCGT 
TTGCAGAGGG TTGTTCTTTG GTTGAACCAA AACTTTCTGT TGCCCGAAGA TGCAGAAATA 
CAGAACGCAC CATTCCAAAT CAGCTTTACA TCGCTGCGTG ATGGAGGAAC CCTCCTACTG 
AGCATGAAAG CCAGTGGAGA GATAAGCCTG CGAACAGATG ATATGGATCT TGCTGGTGAT 
ATCATCCAGT CTTTAGCTTC CTTCTTGGCT ATTGAAGACC TGGCTGTAGA AGCAGATTTT 
CCTAAATACT TTGACCAGCT TCGGAAGGTG CTAGTGCAGG TGGATGAATC TCATTCTGTT 
CATCAGAAGC TGACTGCAGA TATGGCTGAT CAGTCCAATC TAATTCGTAG TATGCTGGTA 
CAAGCTGAAG ACGCTCGCTT AATGAGAGAT ATGAAAAACA TGAAGAAGCG TTACAATGAG 
CTGTATGACC TCAACAGAGA TCTAATAAAT CGTTACAAAA TTCGCTGTAA CAATCATGCA 
GAACTCTTAA ATAATCTTAA ATGTGTAAAC CAGGCAATCC AGCGAGCAGG ACGACTGCGG 
GTTGGGAAGC CCAAAGTCCA GGTGATTACG GCATGCAGGG ATGCAATCCG AAGCAACAAT 
ATCAATGCCT TGTTCAGGAT CATGCGTGTA GGTGCCACAC CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913584.1
CDS	68..2335
Translation	MQTSLSNPNAVYWYPSNHPSVLQPTNSRGPGFIMLVPIFTLKLNHKINPRMVSVGRYDGVHPCLTAATQAGKVFIHNPHSRSQRVSAQRLVQSSQDSDVSLLNINQNVSCLTAGVFDPRVGYDSLIVGTQTNLLAYDVHNNSDLFYKEIVDGANAVVLGSLGDITSPLAVIGGNCALQGFDCSGNDLFWTVTGDNVRSLALCDFDGDEKKELLVGSEDFDIRVFKEDEIVAEMSETETITALCPMYGSRFGYALSNGTVGVYDRTARYWRIKSKNLAMSIHAFDLNSDGVCELITGWSNGKIDARSDRTGEVIFKDNFCSSVAGVVEGDYRMDGKTQLICCSIDGEVRGYLPTSQEAAGSLMDTSVEQELVRELSQKKQNLLLELKNYQENSKQAESRISQSETDGQMGVIPANTQLQTALSVSLGSETQNAHVELSISTSNDTIIRAVLIFAEGIFEGESHVVHPSTQHLSGLVRVPISPPKDVPVDLHIKAFVGYKSSVQFHVFELTRQLPRFSMYVLSNPSSAPETSSYVTFTTSERLQRVVLWLNQNFLLPEDAEIQNAPFQISFTSLRDGGTLLLSMKASGEISLRTDDMDLAGDIIQSLASFLAIEDLAVEADFPKYFDQLRKVLVQVDESHSVHQKLTADMADQSNLIRSMLVQAEDARLMRDMKNMKKRYNELYDLNRDLINRYKIRCNNHAELLNNLKCVNQAIQRAGRLRVGKPKVQVITACRDAIRSNNINALFRIMRVGATPS

Target ORF information:

RefSeq Version	XM_004913527.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913527.3

ORF Insert Sequence:

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAACTTCTTG TAGGATCTGA AGACTTTGAC 
ATCAGAGTGT TTAAAGAGGA TGAAATTGTG GCTGAAATGT CAGAAACTGA GACTATTACA 
GCACTGTGTC CTATGTATGG CAGTCGCTTT GGATATGCCC TCTCCAATGG AACTGTGGGA 
GTGTATGACC GCACAGCTCG GTACTGGAGA ATAAAATCAA AAAATCTTGC AATGAGTATC 
CATGCATTTG ACCTAAACTC TGATGGAGTG TGCGAGCTGA TCACTGGGTG GTCCAATGGA 
AAGATTGATG CCCGTAGTGA TCGCACTGGG GAGGTGATCT TTAAAGATAA CTTCTGCTCC 
TCTGTAGCTG GAGTGGTGGA AGGTGATTAC CGTATGGATG GCAAGACCCA GCTTATCTGC 
TGTTCTATAG ATGGGGAGGT GCGAGGTTAT CTTCCTACCA GTCAGGAGGC CGCAGGAAGT 
CTAATGGATA CAAGCGTGGA ACAGGAACTG GTGAGAGAGC TCAGTCAGAA GAAGCAGAAT 
CTGCTGTTGG AGCTGAAAAA TTATCAAGAA AATTCAAAGC AGGCAGAGTC CCGGATTTCA 
CAGAGTGAGA CTGATGGGCA GATGGGAGTA ATACCAGCAA ACACACAGCT GCAGACTGCT 
CTTTCTGTGT CCCTGGGCTC TGAAACCCAG AATGCACATG TAGAACTAAG CATTTCAACA 
TCAAATGATA CAATCATTCG GGCAGTGCTG ATCTTTGCAG AAGGGATCTT TGAAGGAGAA 
AGTCATGTTG TTCACCCTAG CACTCAGCAT CTAAGTGGCC TGGTTCGTGT TCCGATTAGC 
CCCCCTAAAG ATGTACCAGT GGATCTGCAC ATCAAAGCAT TTGTGGGTTA CAAGAGCAGT 
GTACAGTTCC ATGTATTTGA GCTGACACGG CAGCTTCCTC GCTTTTCCAT GTATGTGCTA 
AGTAATCCTT CCTCAGCACC AGAGACATCC AGTTATGTAA CATTCACCAC CAGTGAGCGT 
TTGCAGAGGG TTGTTCTTTG GTTGAACCAA AACTTTCTGT TGCCCGAAGA TGCAGAAATA 
CAGAACGCAC CATTCCAAAT CAGCTTTACA TCGCTGCGTG ATGGAGGAAC CCTCCTACTG 
AGCATGAAAG CCAGTGGAGA GATAAGCCTG CGAACAGATG ATATGGATCT TGCTGGTGAT 
ATCATCCAGT CTTTAGCTTC CTTCTTGGCT ATTGAAGACC TGGCTGTAGA AGCAGATTTT 
CCTAAATACT TTGACCAGCT TCGGAAGGTG CTAGTGCAGG TGGATGAATC TCATTCTGTT 
CATCAGAAGC TGACTGCAGA TATGGCTGAT CAGTCCAATC TAATTCGTAG TATGCTGGTA 
CAAGCTGAAG ACGCTCGCTT AATGAGAGAT ATGAAAAACA TGAAGAAGCG TTACAATGAG 
CTGTATGACC TCAACAGAGA TCTAATAAAT CGTTACAAAA TTCGCTGTAA CAATCATGCA 
GAACTCTTAA ATAATCTTAA ATGTGTAAAC CAGGCAATCC AGCGAGCAGG ACGACTGCGG 
GTTGGGAAGC CCAAAGTCCA GGTGATTACG GCATGCAGGG ATGCAATCCG AAGCAACAAT 
ATCAATGCCT TGTTCAGGAT CATGCGTGTA GGTGCCACAC CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbs2 ( XM_004913528.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913585.1 Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa08759
Clone ID Related Accession (Same CDS sequence)	XM_004913528.4 , XM_004913528.3
Accession Version	XM_004913528.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Bardet-Biedl syndrome 2 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAACTTCTTG TAGGATCTGA AGACTTTGAC 
ATCAGAGTGT TTAAAGAGGA TGAAATTGTG GCTGAAATGT CAGAAACTGA GACTATTACA 
GCACTGTGTC CTATGTATGG CAGTCGCTTT GGATATGCCC TCTCCAATGG AACTGTGGGA 
GTGTATGACC GCACAGCTCG GTACTGGAGA ATAAAATCAA AAAATCTTGC AATGAGTATC 
CATGCATTTG ACCTAAACTC TGATGGAGTG TGCGAGCTGA TCACTGGGTG GTCCAATGGA 
AAGATTGATG CCCGTAGTGA TCGCACTGGG GAGGTGATCT TTAAAGATAA CTTCTGCTCC 
TCTGTAGCTG GAGTGGTGGA AGGTGATTAC CGTATGGATG GCAAGACCCA GCTTATCTGC 
TGTTCTATAG ATGGGGAGGT GCGAGGTTAT CTTCCTACCA GTCAGGAGGC CGCAGGAAGT 
CTAATGGATA CAAGCGTGGA ACAGGAACTG GTGAGAGAGC TCAGTCAGAA GAAGCAGAAT 
CTGCTGTTGG AGCTGAAAAA TTATCAAGAA AATTCAAAGG CAGAGTCCCG GATTTCACAG 
AGTGAGACTG ATGGGCAGAT GGGAGTAATA CCAGCAAACA CACAGCTGCA GACTGCTCTT 
TCTGTGTCCC TGGGCTCTGA AACCCAGAAT GCACATGTAG AACTAAGCAT TTCAACATCA 
AATGATACAA TCATTCGGGC AGTGCTGATC TTTGCAGAAG GGATCTTTGA AGGAGAAAGT 
CATGTTGTTC ACCCTAGCAC TCAGCATCTA AGTGGCCTGG TTCGTGTTCC GATTAGCCCC 
CCTAAAGATG TACCAGTGGA TCTGCACATC AAAGCATTTG TGGGTTACAA GAGCAGTGTA 
CAGTTCCATG TATTTGAGCT GACACGGCAG CTTCCTCGCT TTTCCATGTA TGTGCTAAGT 
AATCCTTCCT CAGCACCAGA GACATCCAGT TATGTAACAT TCACCACCAG TGAGCGTTTG 
CAGAGGGTTG TTCTTTGGTT GAACCAAAAC TTTCTGTTGC CCGAAGATGC AGAAATACAG 
AACGCACCAT TCCAAATCAG CTTTACATCG CTGCGTGATG GAGGAACCCT CCTACTGAGC 
ATGAAAGCCA GTGGAGAGAT AAGCCTGCGA ACAGATGATA TGGATCTTGC TGGTGATATC 
ATCCAGTCTT TAGCTTCCTT CTTGGCTATT GAAGACCTGG CTGTAGAAGC AGATTTTCCT 
AAATACTTTG ACCAGCTTCG GAAGGTGCTA GTGCAGGTGG ATGAATCTCA TTCTGTTCAT 
CAGAAGCTGA CTGCAGATAT GGCTGATCAG TCCAATCTAA TTCGTAGTAT GCTGGTACAA 
GCTGAAGACG CTCGCTTAAT GAGAGATATG AAAAACATGA AGAAGCGTTA CAATGAGCTG 
TATGACCTCA ACAGAGATCT AATAAATCGT TACAAAATTC GCTGTAACAA TCATGCAGAA 
CTCTTAAATA ATCTTAAATG TGTAAACCAG GCAATCCAGC GAGCAGGACG ACTGCGGGTT 
GGGAAGCCCA AAGTCCAGGT GATTACGGCA TGCAGGGATG CAATCCGAAG CAACAATATC 
AATGCCTTGT TCAGGATCAT GCGTGTAGGT GCCACACCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913585.1
CDS	71..2335
Translation	MQTSLSNPNAVYWYPSNHPSVLQPTNSRGPGFIMLVPIFTLKLNHKINPRMVSVGRYDGVHPCLTAATQAGKVFIHNPHSRSQRVSAQRLVQSSQDSDVSLLNINQNVSCLTAGVFDPRVGYDSLIVGTQTNLLAYDVHNNSDLFYKEIVDGANAVVLGSLGDITSPLAVIGGNCALQGFDCSGNDLFWTVTGDNVRSLALCDFDGDEKKELLVGSEDFDIRVFKEDEIVAEMSETETITALCPMYGSRFGYALSNGTVGVYDRTARYWRIKSKNLAMSIHAFDLNSDGVCELITGWSNGKIDARSDRTGEVIFKDNFCSSVAGVVEGDYRMDGKTQLICCSIDGEVRGYLPTSQEAAGSLMDTSVEQELVRELSQKKQNLLLELKNYQENSKAESRISQSETDGQMGVIPANTQLQTALSVSLGSETQNAHVELSISTSNDTIIRAVLIFAEGIFEGESHVVHPSTQHLSGLVRVPISPPKDVPVDLHIKAFVGYKSSVQFHVFELTRQLPRFSMYVLSNPSSAPETSSYVTFTTSERLQRVVLWLNQNFLLPEDAEIQNAPFQISFTSLRDGGTLLLSMKASGEISLRTDDMDLAGDIIQSLASFLAIEDLAVEADFPKYFDQLRKVLVQVDESHSVHQKLTADMADQSNLIRSMLVQAEDARLMRDMKNMKKRYNELYDLNRDLINRYKIRCNNHAELLNNLKCVNQAIQRAGRLRVGKPKVQVITACRDAIRSNNINALFRIMRVGATPS

Target ORF information:

RefSeq Version	XM_004913528.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913528.3

ORF Insert Sequence:

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAACTTCTTG TAGGATCTGA AGACTTTGAC 
ATCAGAGTGT TTAAAGAGGA TGAAATTGTG GCTGAAATGT CAGAAACTGA GACTATTACA 
GCACTGTGTC CTATGTATGG CAGTCGCTTT GGATATGCCC TCTCCAATGG AACTGTGGGA 
GTGTATGACC GCACAGCTCG GTACTGGAGA ATAAAATCAA AAAATCTTGC AATGAGTATC 
CATGCATTTG ACCTAAACTC TGATGGAGTG TGCGAGCTGA TCACTGGGTG GTCCAATGGA 
AAGATTGATG CCCGTAGTGA TCGCACTGGG GAGGTGATCT TTAAAGATAA CTTCTGCTCC 
TCTGTAGCTG GAGTGGTGGA AGGTGATTAC CGTATGGATG GCAAGACCCA GCTTATCTGC 
TGTTCTATAG ATGGGGAGGT GCGAGGTTAT CTTCCTACCA GTCAGGAGGC CGCAGGAAGT 
CTAATGGATA CAAGCGTGGA ACAGGAACTG GTGAGAGAGC TCAGTCAGAA GAAGCAGAAT 
CTGCTGTTGG AGCTGAAAAA TTATCAAGAA AATTCAAAGG CAGAGTCCCG GATTTCACAG 
AGTGAGACTG ATGGGCAGAT GGGAGTAATA CCAGCAAACA CACAGCTGCA GACTGCTCTT 
TCTGTGTCCC TGGGCTCTGA AACCCAGAAT GCACATGTAG AACTAAGCAT TTCAACATCA 
AATGATACAA TCATTCGGGC AGTGCTGATC TTTGCAGAAG GGATCTTTGA AGGAGAAAGT 
CATGTTGTTC ACCCTAGCAC TCAGCATCTA AGTGGCCTGG TTCGTGTTCC GATTAGCCCC 
CCTAAAGATG TACCAGTGGA TCTGCACATC AAAGCATTTG TGGGTTACAA GAGCAGTGTA 
CAGTTCCATG TATTTGAGCT GACACGGCAG CTTCCTCGCT TTTCCATGTA TGTGCTAAGT 
AATCCTTCCT CAGCACCAGA GACATCCAGT TATGTAACAT TCACCACCAG TGAGCGTTTG 
CAGAGGGTTG TTCTTTGGTT GAACCAAAAC TTTCTGTTGC CCGAAGATGC AGAAATACAG 
AACGCACCAT TCCAAATCAG CTTTACATCG CTGCGTGATG GAGGAACCCT CCTACTGAGC 
ATGAAAGCCA GTGGAGAGAT AAGCCTGCGA ACAGATGATA TGGATCTTGC TGGTGATATC 
ATCCAGTCTT TAGCTTCCTT CTTGGCTATT GAAGACCTGG CTGTAGAAGC AGATTTTCCT 
AAATACTTTG ACCAGCTTCG GAAGGTGCTA GTGCAGGTGG ATGAATCTCA TTCTGTTCAT 
CAGAAGCTGA CTGCAGATAT GGCTGATCAG TCCAATCTAA TTCGTAGTAT GCTGGTACAA 
GCTGAAGACG CTCGCTTAAT GAGAGATATG AAAAACATGA AGAAGCGTTA CAATGAGCTG 
TATGACCTCA ACAGAGATCT AATAAATCGT TACAAAATTC GCTGTAACAA TCATGCAGAA 
CTCTTAAATA ATCTTAAATG TGTAAACCAG GCAATCCAGC GAGCAGGACG ACTGCGGGTT 
GGGAAGCCCA AAGTCCAGGT GATTACGGCA TGCAGGGATG CAATCCGAAG CAACAATATC 
AATGCCTTGT TCAGGATCAT GCGTGTAGGT GCCACACCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbs2 ( XM_002937164.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937210.2 Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X4, mRNA.

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CloneID	OXa08760
Clone ID Related Accession (Same CDS sequence)	XM_002937164.4 , XM_002937164.5
Accession Version	XM_002937164.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Bardet-Biedl syndrome 2 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937164.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGTTC CAATTTTTAC TTTGAAATTA AATCACAAAA TCAATCCGCG CATGGTGTCA 
GTTGGGAGAT ATGATGGAGT ACACCCTTGT TTGACAGCTG CAACCCAAGC TGGAAAGGTT 
TTCATCCATA ACCCCCATTC TCGGAGCCAG CGGGTTTCAG CACAACGTTT GGTGCAGAGC 
AGCCAAGACT CAGATGTGTC ATTGCTGAAC ATTAACCAAA ATGTCAGCTG CTTGACAGCC 
GGAGTGTTTG ACCCACGCGT GGGCTATGAT TCCCTTATAG TGGGAACTCA GACCAACCTT 
TTGGCCTATG ATGTGCACAA TAACTCTGAT TTATTCTATA AGGAGATCGT AGACGGAGCT 
AATGCAGTGG TGCTTGGGAG CTTGGGAGAC ATCACTTCAC CACTTGCTGT CATTGGAGGA 
AACTGTGCTT TGCAAGGATT TGATTGCAGT GGAAATGATC TGTTTTGGAC GGTTACTGGA 
GATAATGTGC GCTCGTTAGC CCTTTGTGAC TTTGATGGAG ATGAGAAGAA GGAACTTCTT 
GTAGGATCTG AAGACTTTGA CATCAGAGTG TTTAAAGAGG ATGAAATTGT GGCTGAAATG 
TCAGAAACTG AGACTATTAC AGCACTGTGT CCTATGTATG GCAGTCGCTT TGGATATGCC 
CTCTCCAATG GAACTGTGGG AGTGTATGAC CGCACAGCTC GGTACTGGAG AATAAAATCA 
AAAAATCTTG CAATGAGTAT CCATGCATTT GACCTAAACT CTGATGGAGT GTGCGAGCTG 
ATCACTGGGT GGTCCAATGG AAAGATTGAT GCCCGTAGTG ATCGCACTGG GGAGGTGATC 
TTTAAAGATA ACTTCTGCTC CTCTGTAGCT GGAGTGGTGG AAGGTGATTA CCGTATGGAT 
GGCAAGACCC AGCTTATCTG CTGTTCTATA GATGGGGAGG TGCGAGGTTA TCTTCCTACC 
AGTCAGGAGG CCGCAGGAAG TCTAATGGAT ACAAGCGTGG AACAGGAACT GGTGAGAGAG 
CTCAGTCAGA AGAAGCAGAA TCTGCTGTTG GAGCTGAAAA ATTATCAAGA AAATTCAAAG 
CAGGCAGAGT CCCGGATTTC ACAGAGTGAG ACTGATGGGC AGATGGGAGT AATACCAGCA 
AACACACAGC TGCAGACTGC TCTTTCTGTG TCCCTGGGCT CTGAAACCCA GAATGCACAT 
GTAGAACTAA GCATTTCAAC ATCAAATGAT ACAATCATTC GGGCAGTGCT GATCTTTGCA 
GAAGGGATCT TTGAAGGAGA AAGTCATGTT GTTCACCCTA GCACTCAGCA TCTAAGTGGC 
CTGGTTCGTG TTCCGATTAG CCCCCCTAAA GATGTACCAG TGGATCTGCA CATCAAAGCA 
TTTGTGGGTT ACAAGAGCAG TGTACAGTTC CATGTATTTG AGCTGACACG GCAGCTTCCT 
CGCTTTTCCA TGTATGTGCT AAGTAATCCT TCCTCAGCAC CAGAGACATC CAGTTATGTA 
ACATTCACCA CCAGTGAGCG TTTGCAGAGG GTTGTTCTTT GGTTGAACCA AAACTTTCTG 
TTGCCCGAAG ATGCAGAAAT ACAGAACGCA CCATTCCAAA TCAGCTTTAC ATCGCTGCGT 
GATGGAGGAA CCCTCCTACT GAGCATGAAA GCCAGTGGAG AGATAAGCCT GCGAACAGAT 
GATATGGATC TTGCTGGTGA TATCATCCAG TCTTTAGCTT CCTTCTTGGC TATTGAAGAC 
CTGGCTGTAG AAGCAGATTT TCCTAAATAC TTTGACCAGC TTCGGAAGGT GCTAGTGCAG 
GTGGATGAAT CTCATTCTGT TCATCAGAAG CTGACTGCAG ATATGGCTGA TCAGTCCAAT 
CTAATTCGTA GTATGCTGGT ACAAGCTGAA GACGCTCGCT TAATGAGAGA TATGAAAAAC 
ATGAAGAAGC GTTACAATGA GCTGTATGAC CTCAACAGAG ATCTAATAAA TCGTTACAAA 
ATTCGCTGTA ACAATCATGC AGAACTCTTA AATAATCTTA AATGTGTAAA CCAGGCAATC 
CAGCGAGCAG GACGACTGCG GGTTGGGAAG CCCAAAGTCC AGGTGATTAC GGCATGCAGG 
GATGCAATCC GAAGCAACAA TATCAATGCC TTGTTCAGGA TCATGCGTGT AGGTGCCACA 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937210.2
CDS	295..2463
Translation	MLVPIFTLKLNHKINPRMVSVGRYDGVHPCLTAATQAGKVFIHNPHSRSQRVSAQRLVQSSQDSDVSLLNINQNVSCLTAGVFDPRVGYDSLIVGTQTNLLAYDVHNNSDLFYKEIVDGANAVVLGSLGDITSPLAVIGGNCALQGFDCSGNDLFWTVTGDNVRSLALCDFDGDEKKELLVGSEDFDIRVFKEDEIVAEMSETETITALCPMYGSRFGYALSNGTVGVYDRTARYWRIKSKNLAMSIHAFDLNSDGVCELITGWSNGKIDARSDRTGEVIFKDNFCSSVAGVVEGDYRMDGKTQLICCSIDGEVRGYLPTSQEAAGSLMDTSVEQELVRELSQKKQNLLLELKNYQENSKQAESRISQSETDGQMGVIPANTQLQTALSVSLGSETQNAHVELSISTSNDTIIRAVLIFAEGIFEGESHVVHPSTQHLSGLVRVPISPPKDVPVDLHIKAFVGYKSSVQFHVFELTRQLPRFSMYVLSNPSSAPETSSYVTFTTSERLQRVVLWLNQNFLLPEDAEIQNAPFQISFTSLRDGGTLLLSMKASGEISLRTDDMDLAGDIIQSLASFLAIEDLAVEADFPKYFDQLRKVLVQVDESHSVHQKLTADMADQSNLIRSMLVQAEDARLMRDMKNMKKRYNELYDLNRDLINRYKIRCNNHAELLNNLKCVNQAIQRAGRLRVGKPKVQVITACRDAIRSNNINALFRIMRVGATPS

Target ORF information:

RefSeq Version	XM_002937164.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937164.4

ORF Insert Sequence:

ATGCTTGTTC CAATTTTTAC TTTGAAATTA AATCACAAAA TCAATCCGCG CATGGTGTCA 
GTTGGGAGAT ATGATGGAGT ACACCCTTGT TTGACAGCTG CAACCCAAGC TGGAAAGGTT 
TTCATCCATA ACCCCCATTC TCGGAGCCAG CGGGTTTCAG CACAACGTTT GGTGCAGAGC 
AGCCAAGACT CAGATGTGTC ATTGCTGAAC ATTAACCAAA ATGTCAGCTG CTTGACAGCC 
GGAGTGTTTG ACCCACGCGT GGGCTATGAT TCCCTTATAG TGGGAACTCA GACCAACCTT 
TTGGCCTATG ATGTGCACAA TAACTCTGAT TTATTCTATA AGGAGATCGT AGACGGAGCT 
AATGCAGTGG TGCTTGGGAG CTTGGGAGAC ATCACTTCAC CACTTGCTGT CATTGGAGGA 
AACTGTGCTT TGCAAGGATT TGATTGCAGT GGAAATGATC TGTTTTGGAC GGTTACTGGA 
GATAATGTGC GCTCGTTAGC CCTTTGTGAC TTTGATGGAG ATGAGAAGAA GGAACTTCTT 
GTAGGATCTG AAGACTTTGA CATCAGAGTG TTTAAAGAGG ATGAAATTGT GGCTGAAATG 
TCAGAAACTG AGACTATTAC AGCACTGTGT CCTATGTATG GCAGTCGCTT TGGATATGCC 
CTCTCCAATG GAACTGTGGG AGTGTATGAC CGCACAGCTC GGTACTGGAG AATAAAATCA 
AAAAATCTTG CAATGAGTAT CCATGCATTT GACCTAAACT CTGATGGAGT GTGCGAGCTG 
ATCACTGGGT GGTCCAATGG AAAGATTGAT GCCCGTAGTG ATCGCACTGG GGAGGTGATC 
TTTAAAGATA ACTTCTGCTC CTCTGTAGCT GGAGTGGTGG AAGGTGATTA CCGTATGGAT 
GGCAAGACCC AGCTTATCTG CTGTTCTATA GATGGGGAGG TGCGAGGTTA TCTTCCTACC 
AGTCAGGAGG CCGCAGGAAG TCTAATGGAT ACAAGCGTGG AACAGGAACT GGTGAGAGAG 
CTCAGTCAGA AGAAGCAGAA TCTGCTGTTG GAGCTGAAAA ATTATCAAGA AAATTCAAAG 
CAGGCAGAGT CCCGGATTTC ACAGAGTGAG ACTGATGGGC AGATGGGAGT AATACCAGCA 
AACACACAGC TGCAGACTGC TCTTTCTGTG TCCCTGGGCT CTGAAACCCA GAATGCACAT 
GTAGAACTAA GCATTTCAAC ATCAAATGAT ACAATCATTC GGGCAGTGCT GATCTTTGCA 
GAAGGGATCT TTGAAGGAGA AAGTCATGTT GTTCACCCTA GCACTCAGCA TCTAAGTGGC 
CTGGTTCGTG TTCCGATTAG CCCCCCTAAA GATGTACCAG TGGATCTGCA CATCAAAGCA 
TTTGTGGGTT ACAAGAGCAG TGTACAGTTC CATGTATTTG AGCTGACACG GCAGCTTCCT 
CGCTTTTCCA TGTATGTGCT AAGTAATCCT TCCTCAGCAC CAGAGACATC CAGTTATGTA 
ACATTCACCA CCAGTGAGCG TTTGCAGAGG GTTGTTCTTT GGTTGAACCA AAACTTTCTG 
TTGCCCGAAG ATGCAGAAAT ACAGAACGCA CCATTCCAAA TCAGCTTTAC ATCGCTGCGT 
GATGGAGGAA CCCTCCTACT GAGCATGAAA GCCAGTGGAG AGATAAGCCT GCGAACAGAT 
GATATGGATC TTGCTGGTGA TATCATCCAG TCTTTAGCTT CCTTCTTGGC TATTGAAGAC 
CTGGCTGTAG AAGCAGATTT TCCTAAATAC TTTGACCAGC TTCGGAAGGT GCTAGTGCAG 
GTGGATGAAT CTCATTCTGT TCATCAGAAG CTGACTGCAG ATATGGCTGA TCAGTCCAAT 
CTAATTCGTA GTATGCTGGT ACAAGCTGAA GACGCTCGCT TAATGAGAGA TATGAAAAAC 
ATGAAGAAGC GTTACAATGA GCTGTATGAC CTCAACAGAG ATCTAATAAA TCGTTACAAA 
ATTCGCTGTA ACAATCATGC AGAACTCTTA AATAATCTTA AATGTGTAAA CCAGGCAATC 
CAGCGAGCAG GACGACTGCG GGTTGGGAAG CCCAAAGTCC AGGTGATTAC GGCATGCAGG 
GATGCAATCC GAAGCAACAA TATCAATGCC TTGTTCAGGA TCATGCGTGT AGGTGCCACA 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbs2 ( XM_012962241.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817695.1 Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X3, mRNA.

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CloneID	OXa35746
Clone ID Related Accession (Same CDS sequence)	XM_012962241.2
Accession Version	XM_012962241.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Bardet-Biedl syndrome 2 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962241.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAAACTATTA CAGCACTGTG TCCTATGTAT 
GGCAGTCGCT TTGGATATGC CCTCTCCAAT GGAACTGTGG GAGTGTATGA CCGCACAGCT 
CGGTACTGGA GAATAAAATC AAAAAATCTT GCAATGAGTA TCCATGCATT TGACCTAAAC 
TCTGATGGAG TGTGCGAGCT GATCACTGGG TGGTCCAATG GAAAGATTGA TGCCCGTAGT 
GATCGCACTG GGGAGGTGAT CTTTAAAGAT AACTTCTGCT CCTCTGTAGC TGGAGTGGTG 
GAAGGTGATT ACCGTATGGA TGGCAAGACC CAGCTTATCT GCTGTTCTAT AGATGGGGAG 
GTGCGAGGTT ATCTTCCTAC CAGTCAGGAG GCCGCAGGAA GTCTAATGGA TACAAGCGTG 
GAACAGGAAC TGGTGAGAGA GCTCAGTCAG AAGAAGCAGA ATCTGCTGTT GGAGCTGAAA 
AATTATCAAG AAAATTCAAA GCAGGCAGAG TCCCGGATTT CACAGAGTGA GACTGATGGG 
CAGATGGGAG TAATACCAGC AAACACACAG CTGCAGACTG CTCTTTCTGT GTCCCTGGGC 
TCTGAAACCC AGAATGCACA TGTAGAACTA AGCATTTCAA CATCAAATGA TACAATCATT 
CGGGCAGTGC TGATCTTTGC AGAAGGGATC TTTGAAGGAG AAAGTCATGT TGTTCACCCT 
AGCACTCAGC ATCTAAGTGG CCTGGTTCGT GTTCCGATTA GCCCCCCTAA AGATGTACCA 
GTGGATCTGC ACATCAAAGC ATTTGTGGGT TACAAGAGCA GTGTACAGTT CCATGTATTT 
GAGCTGACAC GGCAGCTTCC TCGCTTTTCC ATGTATGTGC TAAGTAATCC TTCCTCAGCA 
CCAGAGACAT CCAGTTATGT AACATTCACC ACCAGTGAGC GTTTGCAGAG GGTTGTTCTT 
TGGTTGAACC AAAACTTTCT GTTGCCCGAA GATGCAGAAA TACAGAACGC ACCATTCCAA 
ATCAGCTTTA CATCGCTGCG TGATGGAGGA ACCCTCCTAC TGAGCATGAA AGCCAGTGGA 
GAGATAAGCC TGCGAACAGA TGATATGGAT CTTGCTGGTG ATATCATCCA GTCTTTAGCT 
TCCTTCTTGG CTATTGAAGA CCTGGCTGTA GAAGCAGATT TTCCTAAATA CTTTGACCAG 
CTTCGGAAGG TGCTAGTGCA GGTGGATGAA TCTCATTCTG TTCATCAGAA GCTGACTGCA 
GATATGGCTG ATCAGTCCAA TCTAATTCGT AGTATGCTGG TACAAGCTGA AGACGCTCGC 
TTAATGAGAG ATATGAAAAA CATGAAGAAG CGTTACAATG AGCTGTATGA CCTCAACAGA 
GATCTAATAA ATCGTTACAA AATTCGCTGT AACAATCATG CAGAACTCTT AAATAATCTT 
AAATGTGTAA ACCAGGCAAT CCAGCGAGCA GGACGACTGC GGGTTGGGAA GCCCAAAGTC 
CAGGTGATTA CGGCATGCAG GGATGCAATC CGAAGCAACA ATATCAATGC CTTGTTCAGG 
ATCATGCGTG TAGGTGCCAC ACCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817695.1
CDS	68..2257
Translation	MQTSLSNPNAVYWYPSNHPSVLQPTNSRGPGFIMLVPIFTLKLNHKINPRMVSVGRYDGVHPCLTAATQAGKVFIHNPHSRSQRVSAQRLVQSSQDSDVSLLNINQNVSCLTAGVFDPRVGYDSLIVGTQTNLLAYDVHNNSDLFYKEIVDGANAVVLGSLGDITSPLAVIGGNCALQGFDCSGNDLFWTVTGDNVRSLALCDFDGDEKKETITALCPMYGSRFGYALSNGTVGVYDRTARYWRIKSKNLAMSIHAFDLNSDGVCELITGWSNGKIDARSDRTGEVIFKDNFCSSVAGVVEGDYRMDGKTQLICCSIDGEVRGYLPTSQEAAGSLMDTSVEQELVRELSQKKQNLLLELKNYQENSKQAESRISQSETDGQMGVIPANTQLQTALSVSLGSETQNAHVELSISTSNDTIIRAVLIFAEGIFEGESHVVHPSTQHLSGLVRVPISPPKDVPVDLHIKAFVGYKSSVQFHVFELTRQLPRFSMYVLSNPSSAPETSSYVTFTTSERLQRVVLWLNQNFLLPEDAEIQNAPFQISFTSLRDGGTLLLSMKASGEISLRTDDMDLAGDIIQSLASFLAIEDLAVEADFPKYFDQLRKVLVQVDESHSVHQKLTADMADQSNLIRSMLVQAEDARLMRDMKNMKKRYNELYDLNRDLINRYKIRCNNHAELLNNLKCVNQAIQRAGRLRVGKPKVQVITACRDAIRSNNINALFRIMRVGATPS

Target ORF information:

RefSeq Version	XM_012962241.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962241.2

ORF Insert Sequence:

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAAACTATTA CAGCACTGTG TCCTATGTAT 
GGCAGTCGCT TTGGATATGC CCTCTCCAAT GGAACTGTGG GAGTGTATGA CCGCACAGCT 
CGGTACTGGA GAATAAAATC AAAAAATCTT GCAATGAGTA TCCATGCATT TGACCTAAAC 
TCTGATGGAG TGTGCGAGCT GATCACTGGG TGGTCCAATG GAAAGATTGA TGCCCGTAGT 
GATCGCACTG GGGAGGTGAT CTTTAAAGAT AACTTCTGCT CCTCTGTAGC TGGAGTGGTG 
GAAGGTGATT ACCGTATGGA TGGCAAGACC CAGCTTATCT GCTGTTCTAT AGATGGGGAG 
GTGCGAGGTT ATCTTCCTAC CAGTCAGGAG GCCGCAGGAA GTCTAATGGA TACAAGCGTG 
GAACAGGAAC TGGTGAGAGA GCTCAGTCAG AAGAAGCAGA ATCTGCTGTT GGAGCTGAAA 
AATTATCAAG AAAATTCAAA GCAGGCAGAG TCCCGGATTT CACAGAGTGA GACTGATGGG 
CAGATGGGAG TAATACCAGC AAACACACAG CTGCAGACTG CTCTTTCTGT GTCCCTGGGC 
TCTGAAACCC AGAATGCACA TGTAGAACTA AGCATTTCAA CATCAAATGA TACAATCATT 
CGGGCAGTGC TGATCTTTGC AGAAGGGATC TTTGAAGGAG AAAGTCATGT TGTTCACCCT 
AGCACTCAGC ATCTAAGTGG CCTGGTTCGT GTTCCGATTA GCCCCCCTAA AGATGTACCA 
GTGGATCTGC ACATCAAAGC ATTTGTGGGT TACAAGAGCA GTGTACAGTT CCATGTATTT 
GAGCTGACAC GGCAGCTTCC TCGCTTTTCC ATGTATGTGC TAAGTAATCC TTCCTCAGCA 
CCAGAGACAT CCAGTTATGT AACATTCACC ACCAGTGAGC GTTTGCAGAG GGTTGTTCTT 
TGGTTGAACC AAAACTTTCT GTTGCCCGAA GATGCAGAAA TACAGAACGC ACCATTCCAA 
ATCAGCTTTA CATCGCTGCG TGATGGAGGA ACCCTCCTAC TGAGCATGAA AGCCAGTGGA 
GAGATAAGCC TGCGAACAGA TGATATGGAT CTTGCTGGTG ATATCATCCA GTCTTTAGCT 
TCCTTCTTGG CTATTGAAGA CCTGGCTGTA GAAGCAGATT TTCCTAAATA CTTTGACCAG 
CTTCGGAAGG TGCTAGTGCA GGTGGATGAA TCTCATTCTG TTCATCAGAA GCTGACTGCA 
GATATGGCTG ATCAGTCCAA TCTAATTCGT AGTATGCTGG TACAAGCTGA AGACGCTCGC 
TTAATGAGAG ATATGAAAAA CATGAAGAAG CGTTACAATG AGCTGTATGA CCTCAACAGA 
GATCTAATAA ATCGTTACAA AATTCGCTGT AACAATCATG CAGAACTCTT AAATAATCTT 
AAATGTGTAA ACCAGGCAAT CCAGCGAGCA GGACGACTGC GGGTTGGGAA GCCCAAAGTC 
CAGGTGATTA CGGCATGCAG GGATGCAATC CGAAGCAACA ATATCAATGC CTTGTTCAGG 
ATCATGCGTG TAGGTGCCAC ACCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbs2 ( XM_004913527.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913584.1 Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X1, mRNA.

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CloneID	OXa08758
Clone ID Related Accession (Same CDS sequence)	XM_004913527.4 , XM_004913527.3
Accession Version	XM_004913527.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Bardet-Biedl syndrome 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913527.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAACTTCTTG TAGGATCTGA AGACTTTGAC 
ATCAGAGTGT TTAAAGAGGA TGAAATTGTG GCTGAAATGT CAGAAACTGA GACTATTACA 
GCACTGTGTC CTATGTATGG CAGTCGCTTT GGATATGCCC TCTCCAATGG AACTGTGGGA 
GTGTATGACC GCACAGCTCG GTACTGGAGA ATAAAATCAA AAAATCTTGC AATGAGTATC 
CATGCATTTG ACCTAAACTC TGATGGAGTG TGCGAGCTGA TCACTGGGTG GTCCAATGGA 
AAGATTGATG CCCGTAGTGA TCGCACTGGG GAGGTGATCT TTAAAGATAA CTTCTGCTCC 
TCTGTAGCTG GAGTGGTGGA AGGTGATTAC CGTATGGATG GCAAGACCCA GCTTATCTGC 
TGTTCTATAG ATGGGGAGGT GCGAGGTTAT CTTCCTACCA GTCAGGAGGC CGCAGGAAGT 
CTAATGGATA CAAGCGTGGA ACAGGAACTG GTGAGAGAGC TCAGTCAGAA GAAGCAGAAT 
CTGCTGTTGG AGCTGAAAAA TTATCAAGAA AATTCAAAGC AGGCAGAGTC CCGGATTTCA 
CAGAGTGAGA CTGATGGGCA GATGGGAGTA ATACCAGCAA ACACACAGCT GCAGACTGCT 
CTTTCTGTGT CCCTGGGCTC TGAAACCCAG AATGCACATG TAGAACTAAG CATTTCAACA 
TCAAATGATA CAATCATTCG GGCAGTGCTG ATCTTTGCAG AAGGGATCTT TGAAGGAGAA 
AGTCATGTTG TTCACCCTAG CACTCAGCAT CTAAGTGGCC TGGTTCGTGT TCCGATTAGC 
CCCCCTAAAG ATGTACCAGT GGATCTGCAC ATCAAAGCAT TTGTGGGTTA CAAGAGCAGT 
GTACAGTTCC ATGTATTTGA GCTGACACGG CAGCTTCCTC GCTTTTCCAT GTATGTGCTA 
AGTAATCCTT CCTCAGCACC AGAGACATCC AGTTATGTAA CATTCACCAC CAGTGAGCGT 
TTGCAGAGGG TTGTTCTTTG GTTGAACCAA AACTTTCTGT TGCCCGAAGA TGCAGAAATA 
CAGAACGCAC CATTCCAAAT CAGCTTTACA TCGCTGCGTG ATGGAGGAAC CCTCCTACTG 
AGCATGAAAG CCAGTGGAGA GATAAGCCTG CGAACAGATG ATATGGATCT TGCTGGTGAT 
ATCATCCAGT CTTTAGCTTC CTTCTTGGCT ATTGAAGACC TGGCTGTAGA AGCAGATTTT 
CCTAAATACT TTGACCAGCT TCGGAAGGTG CTAGTGCAGG TGGATGAATC TCATTCTGTT 
CATCAGAAGC TGACTGCAGA TATGGCTGAT CAGTCCAATC TAATTCGTAG TATGCTGGTA 
CAAGCTGAAG ACGCTCGCTT AATGAGAGAT ATGAAAAACA TGAAGAAGCG TTACAATGAG 
CTGTATGACC TCAACAGAGA TCTAATAAAT CGTTACAAAA TTCGCTGTAA CAATCATGCA 
GAACTCTTAA ATAATCTTAA ATGTGTAAAC CAGGCAATCC AGCGAGCAGG ACGACTGCGG 
GTTGGGAAGC CCAAAGTCCA GGTGATTACG GCATGCAGGG ATGCAATCCG AAGCAACAAT 
ATCAATGCCT TGTTCAGGAT CATGCGTGTA GGTGCCACAC CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913584.1
CDS	12..2279
Translation	MQTSLSNPNAVYWYPSNHPSVLQPTNSRGPGFIMLVPIFTLKLNHKINPRMVSVGRYDGVHPCLTAATQAGKVFIHNPHSRSQRVSAQRLVQSSQDSDVSLLNINQNVSCLTAGVFDPRVGYDSLIVGTQTNLLAYDVHNNSDLFYKEIVDGANAVVLGSLGDITSPLAVIGGNCALQGFDCSGNDLFWTVTGDNVRSLALCDFDGDEKKELLVGSEDFDIRVFKEDEIVAEMSETETITALCPMYGSRFGYALSNGTVGVYDRTARYWRIKSKNLAMSIHAFDLNSDGVCELITGWSNGKIDARSDRTGEVIFKDNFCSSVAGVVEGDYRMDGKTQLICCSIDGEVRGYLPTSQEAAGSLMDTSVEQELVRELSQKKQNLLLELKNYQENSKQAESRISQSETDGQMGVIPANTQLQTALSVSLGSETQNAHVELSISTSNDTIIRAVLIFAEGIFEGESHVVHPSTQHLSGLVRVPISPPKDVPVDLHIKAFVGYKSSVQFHVFELTRQLPRFSMYVLSNPSSAPETSSYVTFTTSERLQRVVLWLNQNFLLPEDAEIQNAPFQISFTSLRDGGTLLLSMKASGEISLRTDDMDLAGDIIQSLASFLAIEDLAVEADFPKYFDQLRKVLVQVDESHSVHQKLTADMADQSNLIRSMLVQAEDARLMRDMKNMKKRYNELYDLNRDLINRYKIRCNNHAELLNNLKCVNQAIQRAGRLRVGKPKVQVITACRDAIRSNNINALFRIMRVGATPS

Target ORF information:

RefSeq Version	XM_004913527.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913527.4

ORF Insert Sequence:

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAACTTCTTG TAGGATCTGA AGACTTTGAC 
ATCAGAGTGT TTAAAGAGGA TGAAATTGTG GCTGAAATGT CAGAAACTGA GACTATTACA 
GCACTGTGTC CTATGTATGG CAGTCGCTTT GGATATGCCC TCTCCAATGG AACTGTGGGA 
GTGTATGACC GCACAGCTCG GTACTGGAGA ATAAAATCAA AAAATCTTGC AATGAGTATC 
CATGCATTTG ACCTAAACTC TGATGGAGTG TGCGAGCTGA TCACTGGGTG GTCCAATGGA 
AAGATTGATG CCCGTAGTGA TCGCACTGGG GAGGTGATCT TTAAAGATAA CTTCTGCTCC 
TCTGTAGCTG GAGTGGTGGA AGGTGATTAC CGTATGGATG GCAAGACCCA GCTTATCTGC 
TGTTCTATAG ATGGGGAGGT GCGAGGTTAT CTTCCTACCA GTCAGGAGGC CGCAGGAAGT 
CTAATGGATA CAAGCGTGGA ACAGGAACTG GTGAGAGAGC TCAGTCAGAA GAAGCAGAAT 
CTGCTGTTGG AGCTGAAAAA TTATCAAGAA AATTCAAAGC AGGCAGAGTC CCGGATTTCA 
CAGAGTGAGA CTGATGGGCA GATGGGAGTA ATACCAGCAA ACACACAGCT GCAGACTGCT 
CTTTCTGTGT CCCTGGGCTC TGAAACCCAG AATGCACATG TAGAACTAAG CATTTCAACA 
TCAAATGATA CAATCATTCG GGCAGTGCTG ATCTTTGCAG AAGGGATCTT TGAAGGAGAA 
AGTCATGTTG TTCACCCTAG CACTCAGCAT CTAAGTGGCC TGGTTCGTGT TCCGATTAGC 
CCCCCTAAAG ATGTACCAGT GGATCTGCAC ATCAAAGCAT TTGTGGGTTA CAAGAGCAGT 
GTACAGTTCC ATGTATTTGA GCTGACACGG CAGCTTCCTC GCTTTTCCAT GTATGTGCTA 
AGTAATCCTT CCTCAGCACC AGAGACATCC AGTTATGTAA CATTCACCAC CAGTGAGCGT 
TTGCAGAGGG TTGTTCTTTG GTTGAACCAA AACTTTCTGT TGCCCGAAGA TGCAGAAATA 
CAGAACGCAC CATTCCAAAT CAGCTTTACA TCGCTGCGTG ATGGAGGAAC CCTCCTACTG 
AGCATGAAAG CCAGTGGAGA GATAAGCCTG CGAACAGATG ATATGGATCT TGCTGGTGAT 
ATCATCCAGT CTTTAGCTTC CTTCTTGGCT ATTGAAGACC TGGCTGTAGA AGCAGATTTT 
CCTAAATACT TTGACCAGCT TCGGAAGGTG CTAGTGCAGG TGGATGAATC TCATTCTGTT 
CATCAGAAGC TGACTGCAGA TATGGCTGAT CAGTCCAATC TAATTCGTAG TATGCTGGTA 
CAAGCTGAAG ACGCTCGCTT AATGAGAGAT ATGAAAAACA TGAAGAAGCG TTACAATGAG 
CTGTATGACC TCAACAGAGA TCTAATAAAT CGTTACAAAA TTCGCTGTAA CAATCATGCA 
GAACTCTTAA ATAATCTTAA ATGTGTAAAC CAGGCAATCC AGCGAGCAGG ACGACTGCGG 
GTTGGGAAGC CCAAAGTCCA GGTGATTACG GCATGCAGGG ATGCAATCCG AAGCAACAAT 
ATCAATGCCT TGTTCAGGAT CATGCGTGTA GGTGCCACAC CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbs2 ( XM_004913528.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913585.1 Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X2, mRNA.

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CloneID	OXa08759
Clone ID Related Accession (Same CDS sequence)	XM_004913528.4 , XM_004913528.3
Accession Version	XM_004913528.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Bardet-Biedl syndrome 2 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913528.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAACTTCTTG TAGGATCTGA AGACTTTGAC 
ATCAGAGTGT TTAAAGAGGA TGAAATTGTG GCTGAAATGT CAGAAACTGA GACTATTACA 
GCACTGTGTC CTATGTATGG CAGTCGCTTT GGATATGCCC TCTCCAATGG AACTGTGGGA 
GTGTATGACC GCACAGCTCG GTACTGGAGA ATAAAATCAA AAAATCTTGC AATGAGTATC 
CATGCATTTG ACCTAAACTC TGATGGAGTG TGCGAGCTGA TCACTGGGTG GTCCAATGGA 
AAGATTGATG CCCGTAGTGA TCGCACTGGG GAGGTGATCT TTAAAGATAA CTTCTGCTCC 
TCTGTAGCTG GAGTGGTGGA AGGTGATTAC CGTATGGATG GCAAGACCCA GCTTATCTGC 
TGTTCTATAG ATGGGGAGGT GCGAGGTTAT CTTCCTACCA GTCAGGAGGC CGCAGGAAGT 
CTAATGGATA CAAGCGTGGA ACAGGAACTG GTGAGAGAGC TCAGTCAGAA GAAGCAGAAT 
CTGCTGTTGG AGCTGAAAAA TTATCAAGAA AATTCAAAGG CAGAGTCCCG GATTTCACAG 
AGTGAGACTG ATGGGCAGAT GGGAGTAATA CCAGCAAACA CACAGCTGCA GACTGCTCTT 
TCTGTGTCCC TGGGCTCTGA AACCCAGAAT GCACATGTAG AACTAAGCAT TTCAACATCA 
AATGATACAA TCATTCGGGC AGTGCTGATC TTTGCAGAAG GGATCTTTGA AGGAGAAAGT 
CATGTTGTTC ACCCTAGCAC TCAGCATCTA AGTGGCCTGG TTCGTGTTCC GATTAGCCCC 
CCTAAAGATG TACCAGTGGA TCTGCACATC AAAGCATTTG TGGGTTACAA GAGCAGTGTA 
CAGTTCCATG TATTTGAGCT GACACGGCAG CTTCCTCGCT TTTCCATGTA TGTGCTAAGT 
AATCCTTCCT CAGCACCAGA GACATCCAGT TATGTAACAT TCACCACCAG TGAGCGTTTG 
CAGAGGGTTG TTCTTTGGTT GAACCAAAAC TTTCTGTTGC CCGAAGATGC AGAAATACAG 
AACGCACCAT TCCAAATCAG CTTTACATCG CTGCGTGATG GAGGAACCCT CCTACTGAGC 
ATGAAAGCCA GTGGAGAGAT AAGCCTGCGA ACAGATGATA TGGATCTTGC TGGTGATATC 
ATCCAGTCTT TAGCTTCCTT CTTGGCTATT GAAGACCTGG CTGTAGAAGC AGATTTTCCT 
AAATACTTTG ACCAGCTTCG GAAGGTGCTA GTGCAGGTGG ATGAATCTCA TTCTGTTCAT 
CAGAAGCTGA CTGCAGATAT GGCTGATCAG TCCAATCTAA TTCGTAGTAT GCTGGTACAA 
GCTGAAGACG CTCGCTTAAT GAGAGATATG AAAAACATGA AGAAGCGTTA CAATGAGCTG 
TATGACCTCA ACAGAGATCT AATAAATCGT TACAAAATTC GCTGTAACAA TCATGCAGAA 
CTCTTAAATA ATCTTAAATG TGTAAACCAG GCAATCCAGC GAGCAGGACG ACTGCGGGTT 
GGGAAGCCCA AAGTCCAGGT GATTACGGCA TGCAGGGATG CAATCCGAAG CAACAATATC 
AATGCCTTGT TCAGGATCAT GCGTGTAGGT GCCACACCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913585.1
CDS	13..2277
Translation	MQTSLSNPNAVYWYPSNHPSVLQPTNSRGPGFIMLVPIFTLKLNHKINPRMVSVGRYDGVHPCLTAATQAGKVFIHNPHSRSQRVSAQRLVQSSQDSDVSLLNINQNVSCLTAGVFDPRVGYDSLIVGTQTNLLAYDVHNNSDLFYKEIVDGANAVVLGSLGDITSPLAVIGGNCALQGFDCSGNDLFWTVTGDNVRSLALCDFDGDEKKELLVGSEDFDIRVFKEDEIVAEMSETETITALCPMYGSRFGYALSNGTVGVYDRTARYWRIKSKNLAMSIHAFDLNSDGVCELITGWSNGKIDARSDRTGEVIFKDNFCSSVAGVVEGDYRMDGKTQLICCSIDGEVRGYLPTSQEAAGSLMDTSVEQELVRELSQKKQNLLLELKNYQENSKAESRISQSETDGQMGVIPANTQLQTALSVSLGSETQNAHVELSISTSNDTIIRAVLIFAEGIFEGESHVVHPSTQHLSGLVRVPISPPKDVPVDLHIKAFVGYKSSVQFHVFELTRQLPRFSMYVLSNPSSAPETSSYVTFTTSERLQRVVLWLNQNFLLPEDAEIQNAPFQISFTSLRDGGTLLLSMKASGEISLRTDDMDLAGDIIQSLASFLAIEDLAVEADFPKYFDQLRKVLVQVDESHSVHQKLTADMADQSNLIRSMLVQAEDARLMRDMKNMKKRYNELYDLNRDLINRYKIRCNNHAELLNNLKCVNQAIQRAGRLRVGKPKVQVITACRDAIRSNNINALFRIMRVGATPS

Target ORF information:

RefSeq Version	XM_004913528.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913528.4

ORF Insert Sequence:

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAACTTCTTG TAGGATCTGA AGACTTTGAC 
ATCAGAGTGT TTAAAGAGGA TGAAATTGTG GCTGAAATGT CAGAAACTGA GACTATTACA 
GCACTGTGTC CTATGTATGG CAGTCGCTTT GGATATGCCC TCTCCAATGG AACTGTGGGA 
GTGTATGACC GCACAGCTCG GTACTGGAGA ATAAAATCAA AAAATCTTGC AATGAGTATC 
CATGCATTTG ACCTAAACTC TGATGGAGTG TGCGAGCTGA TCACTGGGTG GTCCAATGGA 
AAGATTGATG CCCGTAGTGA TCGCACTGGG GAGGTGATCT TTAAAGATAA CTTCTGCTCC 
TCTGTAGCTG GAGTGGTGGA AGGTGATTAC CGTATGGATG GCAAGACCCA GCTTATCTGC 
TGTTCTATAG ATGGGGAGGT GCGAGGTTAT CTTCCTACCA GTCAGGAGGC CGCAGGAAGT 
CTAATGGATA CAAGCGTGGA ACAGGAACTG GTGAGAGAGC TCAGTCAGAA GAAGCAGAAT 
CTGCTGTTGG AGCTGAAAAA TTATCAAGAA AATTCAAAGG CAGAGTCCCG GATTTCACAG 
AGTGAGACTG ATGGGCAGAT GGGAGTAATA CCAGCAAACA CACAGCTGCA GACTGCTCTT 
TCTGTGTCCC TGGGCTCTGA AACCCAGAAT GCACATGTAG AACTAAGCAT TTCAACATCA 
AATGATACAA TCATTCGGGC AGTGCTGATC TTTGCAGAAG GGATCTTTGA AGGAGAAAGT 
CATGTTGTTC ACCCTAGCAC TCAGCATCTA AGTGGCCTGG TTCGTGTTCC GATTAGCCCC 
CCTAAAGATG TACCAGTGGA TCTGCACATC AAAGCATTTG TGGGTTACAA GAGCAGTGTA 
CAGTTCCATG TATTTGAGCT GACACGGCAG CTTCCTCGCT TTTCCATGTA TGTGCTAAGT 
AATCCTTCCT CAGCACCAGA GACATCCAGT TATGTAACAT TCACCACCAG TGAGCGTTTG 
CAGAGGGTTG TTCTTTGGTT GAACCAAAAC TTTCTGTTGC CCGAAGATGC AGAAATACAG 
AACGCACCAT TCCAAATCAG CTTTACATCG CTGCGTGATG GAGGAACCCT CCTACTGAGC 
ATGAAAGCCA GTGGAGAGAT AAGCCTGCGA ACAGATGATA TGGATCTTGC TGGTGATATC 
ATCCAGTCTT TAGCTTCCTT CTTGGCTATT GAAGACCTGG CTGTAGAAGC AGATTTTCCT 
AAATACTTTG ACCAGCTTCG GAAGGTGCTA GTGCAGGTGG ATGAATCTCA TTCTGTTCAT 
CAGAAGCTGA CTGCAGATAT GGCTGATCAG TCCAATCTAA TTCGTAGTAT GCTGGTACAA 
GCTGAAGACG CTCGCTTAAT GAGAGATATG AAAAACATGA AGAAGCGTTA CAATGAGCTG 
TATGACCTCA ACAGAGATCT AATAAATCGT TACAAAATTC GCTGTAACAA TCATGCAGAA 
CTCTTAAATA ATCTTAAATG TGTAAACCAG GCAATCCAGC GAGCAGGACG ACTGCGGGTT 
GGGAAGCCCA AAGTCCAGGT GATTACGGCA TGCAGGGATG CAATCCGAAG CAACAATATC 
AATGCCTTGT TCAGGATCAT GCGTGTAGGT GCCACACCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbs2 ( XM_002937164.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937210.2 Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X3, mRNA.

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CloneID	OXa08760
Clone ID Related Accession (Same CDS sequence)	XM_002937164.4 , XM_002937164.5
Accession Version	XM_002937164.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Bardet-Biedl syndrome 2 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937164.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGTTC CAATTTTTAC TTTGAAATTA AATCACAAAA TCAATCCGCG CATGGTGTCA 
GTTGGGAGAT ATGATGGAGT ACACCCTTGT TTGACAGCTG CAACCCAAGC TGGAAAGGTT 
TTCATCCATA ACCCCCATTC TCGGAGCCAG CGGGTTTCAG CACAACGTTT GGTGCAGAGC 
AGCCAAGACT CAGATGTGTC ATTGCTGAAC ATTAACCAAA ATGTCAGCTG CTTGACAGCC 
GGAGTGTTTG ACCCACGCGT GGGCTATGAT TCCCTTATAG TGGGAACTCA GACCAACCTT 
TTGGCCTATG ATGTGCACAA TAACTCTGAT TTATTCTATA AGGAGATCGT AGACGGAGCT 
AATGCAGTGG TGCTTGGGAG CTTGGGAGAC ATCACTTCAC CACTTGCTGT CATTGGAGGA 
AACTGTGCTT TGCAAGGATT TGATTGCAGT GGAAATGATC TGTTTTGGAC GGTTACTGGA 
GATAATGTGC GCTCGTTAGC CCTTTGTGAC TTTGATGGAG ATGAGAAGAA GGAACTTCTT 
GTAGGATCTG AAGACTTTGA CATCAGAGTG TTTAAAGAGG ATGAAATTGT GGCTGAAATG 
TCAGAAACTG AGACTATTAC AGCACTGTGT CCTATGTATG GCAGTCGCTT TGGATATGCC 
CTCTCCAATG GAACTGTGGG AGTGTATGAC CGCACAGCTC GGTACTGGAG AATAAAATCA 
AAAAATCTTG CAATGAGTAT CCATGCATTT GACCTAAACT CTGATGGAGT GTGCGAGCTG 
ATCACTGGGT GGTCCAATGG AAAGATTGAT GCCCGTAGTG ATCGCACTGG GGAGGTGATC 
TTTAAAGATA ACTTCTGCTC CTCTGTAGCT GGAGTGGTGG AAGGTGATTA CCGTATGGAT 
GGCAAGACCC AGCTTATCTG CTGTTCTATA GATGGGGAGG TGCGAGGTTA TCTTCCTACC 
AGTCAGGAGG CCGCAGGAAG TCTAATGGAT ACAAGCGTGG AACAGGAACT GGTGAGAGAG 
CTCAGTCAGA AGAAGCAGAA TCTGCTGTTG GAGCTGAAAA ATTATCAAGA AAATTCAAAG 
CAGGCAGAGT CCCGGATTTC ACAGAGTGAG ACTGATGGGC AGATGGGAGT AATACCAGCA 
AACACACAGC TGCAGACTGC TCTTTCTGTG TCCCTGGGCT CTGAAACCCA GAATGCACAT 
GTAGAACTAA GCATTTCAAC ATCAAATGAT ACAATCATTC GGGCAGTGCT GATCTTTGCA 
GAAGGGATCT TTGAAGGAGA AAGTCATGTT GTTCACCCTA GCACTCAGCA TCTAAGTGGC 
CTGGTTCGTG TTCCGATTAG CCCCCCTAAA GATGTACCAG TGGATCTGCA CATCAAAGCA 
TTTGTGGGTT ACAAGAGCAG TGTACAGTTC CATGTATTTG AGCTGACACG GCAGCTTCCT 
CGCTTTTCCA TGTATGTGCT AAGTAATCCT TCCTCAGCAC CAGAGACATC CAGTTATGTA 
ACATTCACCA CCAGTGAGCG TTTGCAGAGG GTTGTTCTTT GGTTGAACCA AAACTTTCTG 
TTGCCCGAAG ATGCAGAAAT ACAGAACGCA CCATTCCAAA TCAGCTTTAC ATCGCTGCGT 
GATGGAGGAA CCCTCCTACT GAGCATGAAA GCCAGTGGAG AGATAAGCCT GCGAACAGAT 
GATATGGATC TTGCTGGTGA TATCATCCAG TCTTTAGCTT CCTTCTTGGC TATTGAAGAC 
CTGGCTGTAG AAGCAGATTT TCCTAAATAC TTTGACCAGC TTCGGAAGGT GCTAGTGCAG 
GTGGATGAAT CTCATTCTGT TCATCAGAAG CTGACTGCAG ATATGGCTGA TCAGTCCAAT 
CTAATTCGTA GTATGCTGGT ACAAGCTGAA GACGCTCGCT TAATGAGAGA TATGAAAAAC 
ATGAAGAAGC GTTACAATGA GCTGTATGAC CTCAACAGAG ATCTAATAAA TCGTTACAAA 
ATTCGCTGTA ACAATCATGC AGAACTCTTA AATAATCTTA AATGTGTAAA CCAGGCAATC 
CAGCGAGCAG GACGACTGCG GGTTGGGAAG CCCAAAGTCC AGGTGATTAC GGCATGCAGG 
GATGCAATCC GAAGCAACAA TATCAATGCC TTGTTCAGGA TCATGCGTGT AGGTGCCACA 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937210.2
CDS	100..2268
Translation	MLVPIFTLKLNHKINPRMVSVGRYDGVHPCLTAATQAGKVFIHNPHSRSQRVSAQRLVQSSQDSDVSLLNINQNVSCLTAGVFDPRVGYDSLIVGTQTNLLAYDVHNNSDLFYKEIVDGANAVVLGSLGDITSPLAVIGGNCALQGFDCSGNDLFWTVTGDNVRSLALCDFDGDEKKELLVGSEDFDIRVFKEDEIVAEMSETETITALCPMYGSRFGYALSNGTVGVYDRTARYWRIKSKNLAMSIHAFDLNSDGVCELITGWSNGKIDARSDRTGEVIFKDNFCSSVAGVVEGDYRMDGKTQLICCSIDGEVRGYLPTSQEAAGSLMDTSVEQELVRELSQKKQNLLLELKNYQENSKQAESRISQSETDGQMGVIPANTQLQTALSVSLGSETQNAHVELSISTSNDTIIRAVLIFAEGIFEGESHVVHPSTQHLSGLVRVPISPPKDVPVDLHIKAFVGYKSSVQFHVFELTRQLPRFSMYVLSNPSSAPETSSYVTFTTSERLQRVVLWLNQNFLLPEDAEIQNAPFQISFTSLRDGGTLLLSMKASGEISLRTDDMDLAGDIIQSLASFLAIEDLAVEADFPKYFDQLRKVLVQVDESHSVHQKLTADMADQSNLIRSMLVQAEDARLMRDMKNMKKRYNELYDLNRDLINRYKIRCNNHAELLNNLKCVNQAIQRAGRLRVGKPKVQVITACRDAIRSNNINALFRIMRVGATPS

Target ORF information:

RefSeq Version	XM_002937164.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937164.5

ORF Insert Sequence:

ATGCTTGTTC CAATTTTTAC TTTGAAATTA AATCACAAAA TCAATCCGCG CATGGTGTCA 
GTTGGGAGAT ATGATGGAGT ACACCCTTGT TTGACAGCTG CAACCCAAGC TGGAAAGGTT 
TTCATCCATA ACCCCCATTC TCGGAGCCAG CGGGTTTCAG CACAACGTTT GGTGCAGAGC 
AGCCAAGACT CAGATGTGTC ATTGCTGAAC ATTAACCAAA ATGTCAGCTG CTTGACAGCC 
GGAGTGTTTG ACCCACGCGT GGGCTATGAT TCCCTTATAG TGGGAACTCA GACCAACCTT 
TTGGCCTATG ATGTGCACAA TAACTCTGAT TTATTCTATA AGGAGATCGT AGACGGAGCT 
AATGCAGTGG TGCTTGGGAG CTTGGGAGAC ATCACTTCAC CACTTGCTGT CATTGGAGGA 
AACTGTGCTT TGCAAGGATT TGATTGCAGT GGAAATGATC TGTTTTGGAC GGTTACTGGA 
GATAATGTGC GCTCGTTAGC CCTTTGTGAC TTTGATGGAG ATGAGAAGAA GGAACTTCTT 
GTAGGATCTG AAGACTTTGA CATCAGAGTG TTTAAAGAGG ATGAAATTGT GGCTGAAATG 
TCAGAAACTG AGACTATTAC AGCACTGTGT CCTATGTATG GCAGTCGCTT TGGATATGCC 
CTCTCCAATG GAACTGTGGG AGTGTATGAC CGCACAGCTC GGTACTGGAG AATAAAATCA 
AAAAATCTTG CAATGAGTAT CCATGCATTT GACCTAAACT CTGATGGAGT GTGCGAGCTG 
ATCACTGGGT GGTCCAATGG AAAGATTGAT GCCCGTAGTG ATCGCACTGG GGAGGTGATC 
TTTAAAGATA ACTTCTGCTC CTCTGTAGCT GGAGTGGTGG AAGGTGATTA CCGTATGGAT 
GGCAAGACCC AGCTTATCTG CTGTTCTATA GATGGGGAGG TGCGAGGTTA TCTTCCTACC 
AGTCAGGAGG CCGCAGGAAG TCTAATGGAT ACAAGCGTGG AACAGGAACT GGTGAGAGAG 
CTCAGTCAGA AGAAGCAGAA TCTGCTGTTG GAGCTGAAAA ATTATCAAGA AAATTCAAAG 
CAGGCAGAGT CCCGGATTTC ACAGAGTGAG ACTGATGGGC AGATGGGAGT AATACCAGCA 
AACACACAGC TGCAGACTGC TCTTTCTGTG TCCCTGGGCT CTGAAACCCA GAATGCACAT 
GTAGAACTAA GCATTTCAAC ATCAAATGAT ACAATCATTC GGGCAGTGCT GATCTTTGCA 
GAAGGGATCT TTGAAGGAGA AAGTCATGTT GTTCACCCTA GCACTCAGCA TCTAAGTGGC 
CTGGTTCGTG TTCCGATTAG CCCCCCTAAA GATGTACCAG TGGATCTGCA CATCAAAGCA 
TTTGTGGGTT ACAAGAGCAG TGTACAGTTC CATGTATTTG AGCTGACACG GCAGCTTCCT 
CGCTTTTCCA TGTATGTGCT AAGTAATCCT TCCTCAGCAC CAGAGACATC CAGTTATGTA 
ACATTCACCA CCAGTGAGCG TTTGCAGAGG GTTGTTCTTT GGTTGAACCA AAACTTTCTG 
TTGCCCGAAG ATGCAGAAAT ACAGAACGCA CCATTCCAAA TCAGCTTTAC ATCGCTGCGT 
GATGGAGGAA CCCTCCTACT GAGCATGAAA GCCAGTGGAG AGATAAGCCT GCGAACAGAT 
GATATGGATC TTGCTGGTGA TATCATCCAG TCTTTAGCTT CCTTCTTGGC TATTGAAGAC 
CTGGCTGTAG AAGCAGATTT TCCTAAATAC TTTGACCAGC TTCGGAAGGT GCTAGTGCAG 
GTGGATGAAT CTCATTCTGT TCATCAGAAG CTGACTGCAG ATATGGCTGA TCAGTCCAAT 
CTAATTCGTA GTATGCTGGT ACAAGCTGAA GACGCTCGCT TAATGAGAGA TATGAAAAAC 
ATGAAGAAGC GTTACAATGA GCTGTATGAC CTCAACAGAG ATCTAATAAA TCGTTACAAA 
ATTCGCTGTA ACAATCATGC AGAACTCTTA AATAATCTTA AATGTGTAAA CCAGGCAATC 
CAGCGAGCAG GACGACTGCG GGTTGGGAAG CCCAAAGTCC AGGTGATTAC GGCATGCAGG 
GATGCAATCC GAAGCAACAA TATCAATGCC TTGTTCAGGA TCATGCGTGT AGGTGCCACA 
CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbs2 ( XM_018093490.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948979.1 Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X5, mRNA.

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CloneID	OXa44118
Clone ID Related Accession (Same CDS sequence)	XM_018093490.1
Accession Version	XM_018093490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Bardet-Biedl syndrome 2 protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAACTTCTTG TAGGATCTGA AGACTTTGAC 
ATCAGAGTGT TTAAAGAGGA TGAAATTGTG GCTGAAATGT CAGAAACTGA GACTATTACA 
GCACTGTGTC CTATGTATGG CAGTCGCTTT GGATATGCCC TCTCCAATGG AACTGTGGGA 
GTGTATGACC GCACAGCTCG GTACTGGAGA ATAAAATCAA AAAATCTTGC AATGAGTATC 
CATGCATTTG ACCTAAACTC TGATGGAGTG TGCGAGCTGA TCACTGGGTG GTCCAATGGA 
AAGATTGATG CCCGTAGTGA TCGCACTGGG GAGGTGATCT TTAAAGATAA CTTCTGCTCC 
TCTGTAGCTG GAGTGGTGGA AGGTGATTAC CGTATGGATG GCAAGACCCA GCTTATCTGC 
TGTTCTATAG ATGGGGAGGT GCGAGGTTAT CTTCCTACCA GTCAGGAGGC CGCAGGAAGT 
CTAATGGATA CAAGCGTGGA ACAGGAACTG GTGAGAGAGC TCAGTCAGAA GAAGCAGAAT 
CTGCTGTTGG AGCTGAAAAA TTATCAAGAA AATTCAAAGC AGGCAGAGTC CCGGATTTCA 
CAGAGTGAGA CTGATGGGCA GATGGGAGTA ATACCAGCAA ACACACAGCT GCAGACTGCT 
CTTTCTGTGT CCCTGGGCTC TGAAACCCAG AATGCACATG TAGAACTAAG CATTTCAACA 
TCAAATGATA CAATCATTCG GGCAGTGCTG ATCTTTGCAG AAGGGATCTT TGAAGGAGAA 
AGTCATGTTG TTCACCCTAG CACTCAGCAT CTAAGTGGCC TGGTTCGTGT TCCGATTAGC 
CCCCCTAAAG ATGTACCAGT GGATCTGCAC ATCAAAGCAT TTGTGGGTTA CAAGAGCAGT 
GTACAGTTCC ATGTATTTGA GCTGACACGG CAGCTTCCTC GCTTTTCCAT GTATGTGCTA 
AGTAATCCTT CCTCAGCACC AGAGACATCC AGTTATGTAA CATTCACCAC CAGTGAGCGT 
TTGCAGAGGA TAAGCCTGCG AACAGATGAT ATGGATCTTG CTGGTGATAT CATCCAGTCT 
TTAGCTTCCT TCTTGGCTAT TGAAGACCTG GCTGTAGAAG CAGATTTTCC TAAATACTTT 
GACCAGCTTC GGAAGGTGCT AGTGCAGGTG GATGAATCTC ATTCTGTTCA TCAGAAGCTG 
ACTGCAGATA TGGCTGATCA GTCCAATCTA ATTCGTAGTA TGCTGGTACA AGCTGAAGAC 
GCTCGCTTAA TGAGAGATAT GAAAAACATG AAGAAGCGTT ACAATGAGCT GTATGACCTC 
AACAGAGATC TAATAAATCG TTACAAAATT CGCTGTAACA ATCATGCAGA ACTCTTAAAT 
AATCTTAAAT GTGTAAACCA GGCAATCCAG CGAGCAGGAC GACTGCGGGT TGGGAAGCCC 
AAAGTCCAGG TGATTACGGC ATGCAGGGAT GCAATCCGAA GCAACAATAT CAATGCCTTG 
TTCAGGATCA TGCGTGTAGG TGCCACACCC TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948979.1
CDS	71..2206
Translation	MQTSLSNPNAVYWYPSNHPSVLQPTNSRGPGFIMLVPIFTLKLNHKINPRMVSVGRYDGVHPCLTAATQAGKVFIHNPHSRSQRVSAQRLVQSSQDSDVSLLNINQNVSCLTAGVFDPRVGYDSLIVGTQTNLLAYDVHNNSDLFYKEIVDGANAVVLGSLGDITSPLAVIGGNCALQGFDCSGNDLFWTVTGDNVRSLALCDFDGDEKKELLVGSEDFDIRVFKEDEIVAEMSETETITALCPMYGSRFGYALSNGTVGVYDRTARYWRIKSKNLAMSIHAFDLNSDGVCELITGWSNGKIDARSDRTGEVIFKDNFCSSVAGVVEGDYRMDGKTQLICCSIDGEVRGYLPTSQEAAGSLMDTSVEQELVRELSQKKQNLLLELKNYQENSKQAESRISQSETDGQMGVIPANTQLQTALSVSLGSETQNAHVELSISTSNDTIIRAVLIFAEGIFEGESHVVHPSTQHLSGLVRVPISPPKDVPVDLHIKAFVGYKSSVQFHVFELTRQLPRFSMYVLSNPSSAPETSSYVTFTTSERLQRISLRTDDMDLAGDIIQSLASFLAIEDLAVEADFPKYFDQLRKVLVQVDESHSVHQKLTADMADQSNLIRSMLVQAEDARLMRDMKNMKKRYNELYDLNRDLINRYKIRCNNHAELLNNLKCVNQAIQRAGRLRVGKPKVQVITACRDAIRSNNINALFRIMRVGATPS

Target ORF information:

RefSeq Version	XM_018093490.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Bardet-Biedl syndrome 2 (bbs2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093490.1

ORF Insert Sequence:

ATGCAAACCA GCCTGTCCAA TCCCAATGCT GTTTACTGGT ATCCTAGCAA CCACCCCTCC 
GTTCTGCAGC CCACTAACAG CAGGGGCCCG GGATTCATCA TGCTTGTTCC AATTTTTACT 
TTGAAATTAA ATCACAAAAT CAATCCGCGC ATGGTGTCAG TTGGGAGATA TGATGGAGTA 
CACCCTTGTT TGACAGCTGC AACCCAAGCT GGAAAGGTTT TCATCCATAA CCCCCATTCT 
CGGAGCCAGC GGGTTTCAGC ACAACGTTTG GTGCAGAGCA GCCAAGACTC AGATGTGTCA 
TTGCTGAACA TTAACCAAAA TGTCAGCTGC TTGACAGCCG GAGTGTTTGA CCCACGCGTG 
GGCTATGATT CCCTTATAGT GGGAACTCAG ACCAACCTTT TGGCCTATGA TGTGCACAAT 
AACTCTGATT TATTCTATAA GGAGATCGTA GACGGAGCTA ATGCAGTGGT GCTTGGGAGC 
TTGGGAGACA TCACTTCACC ACTTGCTGTC ATTGGAGGAA ACTGTGCTTT GCAAGGATTT 
GATTGCAGTG GAAATGATCT GTTTTGGACG GTTACTGGAG ATAATGTGCG CTCGTTAGCC 
CTTTGTGACT TTGATGGAGA TGAGAAGAAG GAACTTCTTG TAGGATCTGA AGACTTTGAC 
ATCAGAGTGT TTAAAGAGGA TGAAATTGTG GCTGAAATGT CAGAAACTGA GACTATTACA 
GCACTGTGTC CTATGTATGG CAGTCGCTTT GGATATGCCC TCTCCAATGG AACTGTGGGA 
GTGTATGACC GCACAGCTCG GTACTGGAGA ATAAAATCAA AAAATCTTGC AATGAGTATC 
CATGCATTTG ACCTAAACTC TGATGGAGTG TGCGAGCTGA TCACTGGGTG GTCCAATGGA 
AAGATTGATG CCCGTAGTGA TCGCACTGGG GAGGTGATCT TTAAAGATAA CTTCTGCTCC 
TCTGTAGCTG GAGTGGTGGA AGGTGATTAC CGTATGGATG GCAAGACCCA GCTTATCTGC 
TGTTCTATAG ATGGGGAGGT GCGAGGTTAT CTTCCTACCA GTCAGGAGGC CGCAGGAAGT 
CTAATGGATA CAAGCGTGGA ACAGGAACTG GTGAGAGAGC TCAGTCAGAA GAAGCAGAAT 
CTGCTGTTGG AGCTGAAAAA TTATCAAGAA AATTCAAAGC AGGCAGAGTC CCGGATTTCA 
CAGAGTGAGA CTGATGGGCA GATGGGAGTA ATACCAGCAA ACACACAGCT GCAGACTGCT 
CTTTCTGTGT CCCTGGGCTC TGAAACCCAG AATGCACATG TAGAACTAAG CATTTCAACA 
TCAAATGATA CAATCATTCG GGCAGTGCTG ATCTTTGCAG AAGGGATCTT TGAAGGAGAA 
AGTCATGTTG TTCACCCTAG CACTCAGCAT CTAAGTGGCC TGGTTCGTGT TCCGATTAGC 
CCCCCTAAAG ATGTACCAGT GGATCTGCAC ATCAAAGCAT TTGTGGGTTA CAAGAGCAGT 
GTACAGTTCC ATGTATTTGA GCTGACACGG CAGCTTCCTC GCTTTTCCAT GTATGTGCTA 
AGTAATCCTT CCTCAGCACC AGAGACATCC AGTTATGTAA CATTCACCAC CAGTGAGCGT 
TTGCAGAGGA TAAGCCTGCG AACAGATGAT ATGGATCTTG CTGGTGATAT CATCCAGTCT 
TTAGCTTCCT TCTTGGCTAT TGAAGACCTG GCTGTAGAAG CAGATTTTCC TAAATACTTT 
GACCAGCTTC GGAAGGTGCT AGTGCAGGTG GATGAATCTC ATTCTGTTCA TCAGAAGCTG 
ACTGCAGATA TGGCTGATCA GTCCAATCTA ATTCGTAGTA TGCTGGTACA AGCTGAAGAC 
GCTCGCTTAA TGAGAGATAT GAAAAACATG AAGAAGCGTT ACAATGAGCT GTATGACCTC 
AACAGAGATC TAATAAATCG TTACAAAATT CGCTGTAACA ATCATGCAGA ACTCTTAAAT 
AATCTTAAAT GTGTAAACCA GGCAATCCAG CGAGCAGGAC GACTGCGGGT TGGGAAGCCC 
AAAGTCCAGG TGATTACGGC ATGCAGGGAT GCAATCCGAA GCAACAATAT CAATGCCTTG 
TTCAGGATCA TGCGTGTAGG TGCCACACCC TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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bbs2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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