Pbrm1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pbrm1
Entrez Gene ID	66923
Full Name	polybromo 1
Synonyms	2610016F04Rik,AI507524,BAF180,Pb1
General protein information	Preferred Names polybromo 1 Names protein polybromo-1 2310032M22Rik BRG1-associated factor 180
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 B

mRNA and Protein(s)

mRNA	Protein	Name
XM_017316156.1	XP_017171645.1	protein polybromo-1 isoform X24
XM_006519403.1	XP_006519466.1	protein polybromo-1 isoform X1
XM_006519405.3	XP_006519468.1	protein polybromo-1 isoform X1
XM_017316139.1	XP_017171628.1	protein polybromo-1 isoform X2
XM_006519407.3	XP_006519470.1	protein polybromo-1 isoform X3
XM_006519408.3	XP_006519471.1	protein polybromo-1 isoform X4
XM_017316143.1	XP_017171632.1	protein polybromo-1 isoform X6
XM_006519414.3	XP_006519477.1	protein polybromo-1 isoform X9
XM_017316151.1	XP_017171640.1	protein polybromo-1 isoform X12
XM_006519425.3	XP_006519488.1	protein polybromo-1 isoform X29
NM_001359857.1	NP_001346786.1	protein polybromo-1 isoform 5
XM_006519411.3	XP_006519474.1	protein polybromo-1 isoform X8
XM_017316146.1	XP_017171635.1	protein polybromo-1 isoform X9
XM_006519413.3	XP_006519476.1	protein polybromo-1 isoform X9
XM_017316148.1	XP_017171637.1	protein polybromo-1 isoform X11
XM_006519418.3	XP_006519481.1	protein polybromo-1 isoform X19
XM_017316158.1	XP_017171647.1	protein polybromo-1 isoform X25
NM_001359855.1	NP_001346784.1	protein polybromo-1 isoform 3
NM_001359854.1	NP_001346783.1	protein polybromo-1 isoform 2
XM_017316137.1	XP_017171626.1	protein polybromo-1 isoform X1
XM_006519410.3	XP_006519473.1	protein polybromo-1 isoform X6
XM_017316150.1	XP_017171639.1	protein polybromo-1 isoform X12
XM_017316154.1	XP_017171643.1	protein polybromo-1 isoform X17
XM_006519421.3	XP_006519484.1	protein polybromo-1 isoform X22
XM_006519422.3	XP_006519485.1	protein polybromo-1 isoform X23
XM_006519402.3	XP_006519465.1	protein polybromo-1 isoform X1
XM_017316138.1	XP_017171627.1	protein polybromo-1 isoform X1
XM_017316140.1	XP_017171629.1	protein polybromo-1 isoform X3
XM_017316144.1	XP_017171633.1	protein polybromo-1 isoform X6
XM_017316145.1	XP_017171634.1	protein polybromo-1 isoform X7
XM_017316147.1	XP_017171636.1	protein polybromo-1 isoform X10
XM_017316153.1	XP_017171642.1	protein polybromo-1 isoform X15
XM_017316155.1	XP_017171644.1	protein polybromo-1 isoform X18
XM_006519419.2	XP_006519482.1	protein polybromo-1 isoform X20
XM_006519427.3	XP_006519490.1	protein polybromo-1 isoform X31
XM_017316160.1	XP_017171649.1	protein polybromo-1 isoform X32
NM_001081251.1	NP_001074720.1	protein polybromo-1 isoform 1
NM_001359856.1	NP_001346785.1	protein polybromo-1 isoform 4
XM_006519406.3	XP_006519469.1	protein polybromo-1 isoform X2
XM_017316141.1	XP_017171630.1	protein polybromo-1 isoform X3
XM_017316142.1	XP_017171631.1	protein polybromo-1 isoform X4
XM_006519409.3	XP_006519472.1	protein polybromo-1 isoform X5
XM_006519412.3	XP_006519475.1	protein polybromo-1 isoform X9
XM_006519415.3	XP_006519478.1	protein polybromo-1 isoform X10
XM_017316149.1	XP_017171638.1	protein polybromo-1 isoform X11
XM_006519416.1	XP_006519479.1	protein polybromo-1 isoform X13
XM_017316152.1	XP_017171641.1	protein polybromo-1 isoform X14
XM_006519417.1	XP_006519480.1	protein polybromo-1 isoform X16
XM_006519420.3	XP_006519483.1	protein polybromo-1 isoform X21
XM_017316157.1	XP_017171646.1	protein polybromo-1 isoform X25
XM_006519423.3	XP_006519486.1	protein polybromo-1 isoform X26
XM_017316159.1	XP_017171648.1	protein polybromo-1 isoform X27
XM_006519424.3	XP_006519487.1	protein polybromo-1 isoform X28
XM_006519426.3	XP_006519489.1	protein polybromo-1 isoform X30
NM_001359858.1	NP_001346787.1	protein polybromo-1 isoform 5

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	polybromo	NP_651288.1
Xenopus tropicalis (tropical clawed frog)	pbrm1	XP_004919938.1
Homo sapiens (human)	PBRM1	NP_060783.3
	PBRM1	XP_001088172.2
Rattus norvegicus (Norway rat)	Pbrm1	XP_006252749.1
Canis lupus familiaris (dog)	PBRM1	XP_005632499.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001099	XP_322068.4
Pan troglodytes (chimpanzee)	PBRM1	XP_516515.3
Mus musculus (house mouse)	Pbrm1	NP_001074720.1
Gallus gallus (chicken)	PBRM1	NP_990496.1
Bos taurus (cattle)	PBRM1	XP_005223037.1
Danio rerio (zebrafish)	pbrm1	XP_693735.5
Caenorhabditis elegans (roundworm)	pbrm-1	NP_001021008.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of PBRM1 rescues VHL dependent replication stress to promote renal carcinogenesis.
Espana-Agusti J, Warren A, Chew SK, Adams DJ, Matakidou A
Nature communications8(1)2026(2017 Dec)

Leung JY, Kim WY
Cancer discovery7(8)802-804(2017 08)

Modeling Renal Cell Carcinoma in Mice:
Gu YF, Cohn S, Christie A, McKenzie T, Wolff N, Do QN, Madhuranthakam AJ, Pedrosa I, Wang T, Dey A, Busslinger M, Xie XJ, Hammer RE, McKay RM, Kapur P, Brugarolas J
Cancer discovery7(8)900-917(2017 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

kidney-specific deletion of Vhl and Pbrm1, but not either gene alone, results in bilateral, multifocal, transplantable clear cell kidney cancers.
Title: The SWI/SNF Protein PBRM1 Restrains VHL-Loss-Driven Clear Cell Renal Cell Carcinoma.

Data suggest BAF180 protein as a critical regulator of cellular senescence and HSC homeostasis, which is at least partially regulated through BAF180-mediated suppression of cell cycle regulator p21 expression.
Title: BAF180 regulates cellular senescence and hematopoietic stem cell homeostasis through p21.

Data demonstrate a function for BAF180 in promoting genome stability that is distinct from its well-characterized role in transcriptional regulation.
Title: BAF180 promotes cohesion and prevents genome instability and aneuploidy.

BAF180 is a repressor of IL-10 transcription in Th2 cells and suggest that the differential recruitment of different SWI/SNF subtypes can have direct consequences on chromatin structure and gene transcription.
Title: IL-10 transcription is negatively regulated by BAF180, a component of the SWI/SNF chromatin remodeling enzyme.

BAF180 is critical for coronary vessel formation.
Title: Coronary development is regulated by ATP-dependent SWI/SNF chromatin remodeling component BAF180.

The following Pbrm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pbrm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu78833	NM_001359854.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78836	NM_001359858.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu78836	NM_001359857.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu84287	NM_001359855.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78826	XM_017316145.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78827	XM_017316148.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78827	XM_017316149.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78828	XM_017316151.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78828	XM_017316150.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78829	XM_017316152.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78830	XM_017316153.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78831	XM_017316154.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78832	XM_017316155.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78833	XM_017316156.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78834	XM_017316158.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X42, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu78834	XM_017316157.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu78835	XM_017316159.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X44, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu78836	XM_017316160.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X49, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu01967	NM_001081251.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50273	NM_001359856.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu50253	XM_006519403.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50253	XM_017316137.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50253	XM_006519405.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50253	XM_017316138.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50253	XM_006519402.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50254	XM_017316139.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50254	XM_006519406.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50255	XM_006519407.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50255	XM_017316141.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50255	XM_017316140.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50256	XM_017316142.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50256	XM_006519408.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50257	XM_006519409.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50258	XM_017316143.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50258	XM_006519410.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50258	XM_017316144.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50259	XM_006519411.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50260	XM_006519412.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50260	XM_017316146.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50260	XM_006519414.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50260	XM_006519413.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50261	XM_017316147.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50261	XM_006519415.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50262	XM_006519416.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50263	XM_006519417.1 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50264	XM_006519418.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50265	XM_006519419.2 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50266	XM_006519420.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50267	XM_006519421.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50268	XM_006519422.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu50269	XM_006519423.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X43, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu50270	XM_006519424.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X45, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu50271	XM_006519425.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X46, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu50272	XM_006519426.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X47, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu50273	XM_006519427.3 Latest version!	Mus musculus polybromo 1 (Pbrm1), transcript variant X48, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pbrm1 ( NM_001359854.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346783.1 Mus musculus polybromo 1 (Pbrm1), transcript variant 2, mRNA.

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CloneID	OMu78833
Clone ID Related Accession (Same CDS sequence)	XM_017316156.1 , NM_001359854.1
Accession Version	NM_001359854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154727.2, AK166588.1, AK165182.1, AI615867.1, CA557251.1, AI507524.1, CD803060.1, BG065752.2, CN707140.1, CN530699.1, CD803036.1, CN537785.1, BQ558912.1, AC154446.2 and AW048644.1. On Jan 18, 2018 this sequence version replaced XM_017316156.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.50442.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGAGC GGGCAGCTAA AGTTGCTGAG CAGCAGGAGA GAGAGCGAGC AGCACAGCAA 
CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG 
CCACCACCAA CACCAATGGG GATGCTCAAT CAGCAGTTGA CACCTGTTGC AGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346783.1
CDS	436..5448
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	NM_001359854.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359854.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGAGC GGGCAGCTAA AGTTGCTGAG CAGCAGGAGA GAGAGCGAGC AGCACAGCAA 
CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG 
CCACCACCAA CACCAATGGG GATGCTCAAT CAGCAGTTGA CACCTGTTGC AGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( NM_001359857.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346786.1 Mus musculus polybromo 1 (Pbrm1), transcript variant 5, mRNA.

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CloneID	OMu78836
Clone ID Related Accession (Same CDS sequence)	NM_001359857.1 , XM_017316160.1 , NM_001359858.1
Accession Version	NM_001359857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154727.2, AK166588.1, AK165182.1, AI615867.1, AK009582.1, CD803060.1, BG065752.2, CN707140.1, CN530699.1, CD803036.1, CN537785.1, BQ558912.1, AC154446.2 and AW048644.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660825.97440.1, SRR1660819.88093.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGGA ATACTCATGA CAGTGAGATG TTGGGTCTCA GGAGGCTATC CAGTAAAAAG 
AACATAAGAA AACAGCGAAT GAAAATTTTA TTCAATGTTG TTCTTGAAGC TCGAGAGCCA 
GGTTCAGGCA GAAGACTTTG CGATCTATTT ATGGTTAAGC CATCCAAGAA GGACTATCCT 
GATTATTATA AAATCATCTT AGAGCCAATG GACCTGAAAA TAATTGAGCA TAACATCCGA 
AATGACAAAT ATGCAGGTGA AGAAGGAATG ATGGAAGACA TGAAACTCAT GTTCCGCAAT 
GCCAGGCACT ACAATGAGGA GGGCTCCCAG GTATACAATG ATGCCCATAT CCTGGAGAAG 
TTACTCAAAG ATAAAAGGAA AGAGCTGGGC CCTCTGCCTG ATGATGATGA CATGGCTTCT 
CCCAAACTTA AATTGAGTAG GAAGAGTGGT GTTTCTCCTA AGAAATCAAA GTACATGACT 
CCAATGCAGC AGAAACTGAA TGAAGTGTAT GAAGCTGTAA AGAACTATAC TGATAAGAGG 
GGTCGCCGCC TTAGTGCTAT ATTTCTAAGA CTCCCCTCTA GATCAGAGCT GCCTGACTAC 
TACCTGACCA TTAAAAAGCC CATGGACATG GAAAAAATTC GAAGTCACAT GATGGCAAAC 
AAGTACCAAG ACATAGATTC TATGGTAGAG GACTTTGTCA TGATGTTTAA TAATGCCTGT 
ACCTACAATG AACCAGAGTC TTTGATCTAC AAAGATGCCC TTGTACTGCA TAAAGTCCTC 
CTTGAGACTC GGAGAGACCT GGAGGGAGAT GAGGATTCTC ATGTCCCTAA TGTGACGTTG 
CTGATTCAAG AGCTCATCCA TAACCTTTTT GTGTCAGTCA TGAGTCATCA GGATGACGAA 
GGGAGGTGTT ACAGCGACTC CTTAGCAGAA ATTCCTGCTG TGGATCCCAA CTCTCCCAAT 
AAACCTCCCC TTACATTTGA CATTATCAGG AAAAATGTTG AAAGTAATCG GTATCGGCGA 
CTTGATTTAT TTCAGGAGCA TATGTTTGAA GTATTGGAAC GGGCAAGAAG GATGAACCGG 
ACAGATTCCG AAATATATGA GGATGCTGTA GAACTTCAGC AGTTTTTTAT TAGAATTCGT 
GATGAACTCT GCAAAAATGG AGAGATCCTT CTTTCTCCAG CACTCAGCTA TACCACAAAA 
CACTTGCATA ACGATGTGGA AAAAGAAAAA AAGGAAAAAT TGCCTAAAGA AATAGAGGAA 
GATAAACTAA AACGCGAAGA AGAAAAAAGA GAAGCTGAAA AAAGTGAAGA TTCCTCAGGT 
ACTACAGGCC TCTCAGGCTT ACATCGTACA TACAGCCAGG ACTGCAGCTT TAAGAACAGC 
ATGTATCATG TCGGAGATTA TGTCTATGTT GAACCTGCGG AGGCCAATCT ACAACCACAT 
ATAGTGTGTA TTGAGAGACT GTGGGAGGAT TCAGCTGGTG AAAAATGGTT GTACGGCTGT 
TGGTTTTATC GGCCAAATGA AACATTCCAT TTGGCTACAC GAAAATTTCT AGAAAAAGAA 
GTTTTTAAGA GTGACTACTA CAATAAAGTA CCTGTTAGTA AAATTCTAGG CAAATGTGTA 
GTCATGTTTG TCAAGGAATA CTTTAAATTA TGTCCAGAAA ACTTTCGCGA TGAGGATGTT 
TTTGTCTGTG AATCGAGGTA TTCTGCCAAA ACCAAATCTT TTAAGAAAAT TAAACTGTGG 
ACCATGCCCA TCAGTTCAGT TAGATTTGTC CCTCGGGATG TGCCTTTGCC TGTGGTCCGA 
GTGGCCTCTG TGTTTGCAAA TGCAGATAAA GGGGATGATG AGAAGAATAC AGACAACTCA 
GATGACAATA GAGCTGAAGA CAATTTTAAC TTGGAAAAGG AAAAAGAAGA TGTTCCTGTG 
GAGATGTCCA ATGGTGAGCC AGGTTGCCAC TACTTTGAGC AGCTTCGGTA CAATGACATG 
TGGCTGAAGG TTGGTGATTG TGTCTTCATC AAATCCCACG GCTTGGTGCG CCCTCGTGTG 
GGCAGAATTG AGAAAGTATG GGTCCGAGAT GGAGCTGCAT ATTTTTATGG CCCTATCTTC 
ATTCATCCAG AAGAAACAGA ACATGAGCCC ACAAAAATGT TCTACAAAAA AGAAGTGTTT 
CTGAGTAATC TGGAAGAGAC CTGCCCTATG AGTTGTATTC TGGGGAAATG TGCAGTGCTG 
TCATTCAAGG ACTTCCTCTC CTGCAGGCCA ACTGAAATAC CAGAAAATGA CATTCTGCTT 
TGTGAGAGCC GCTATAATGA GAGTGACAAG CAGATGAAGA AGTTCAAGGG TTTGAAGAGG 
TTTTCACTCT CTGCTAAAGT TGTAGATGAT GAAATCTACT ACTTCAGAAA ACCAATCATT 
CCTCAGAAGG AACCCTCACC TTTGTTAGAA AAGAAGATAC AATTGCTAGA AGCTAAATTT 
GCAGAGTTAG AAGGAGGAGA TGATGATATT GAGGAGATGG GAGAAGAGGA TAGTGAAGTC 
ATTGAAGCTC CATCTCTACC TCAACTGCAG ACACCCCTGG CCAATGAGTT GGACCTCATG 
CCCTATACAC CCCCACAGTC TACCCCAAAG TCTGCCAAAG GCAGTGCAAA GAAGGAAAGT 
TCTAAACGAA AAATCAACAT GAGTGGCTAC ATTTTGTTCA GCAGTGAAAT GAGAGCTGTG 
ATTAAAGCCC AGCACCCAGA CTACTCTTTT GGGGAGCTCA GCAGACTGGT GGGGACAGAA 
TGGAGAAACC TTGAAACAGC CAAGAAAGCA GAATATGAAG GTGTGATGAA TCAAGGAGTA 
GCCCCCATGG TAGGGACTCC AGCACCAGGT GGAAGTCCGT ATGGACAACA GGTAGGAGTT 
TTGGGACCTC CAGGGCAGCA GGCACCACCT CCATATCCTG GTCCTCATCC AGCTGGCCCC 
CCTGTCATAC AGCAGCCAAC AACGCCCATG TTTGTGGCTC CCCCACCAAA GACCCAAAGG 
CTTCTCCACT CAGAGGCCTA CCTGAAATAC ATTGAAGGAC TCAGTGCTGA ATCCAACAGC 
ATTAGCAAGT GGGACCAAAC TTTGGCAGCT CGAAGACGGG ATGTCCATTT GTCCAAAGAA 
CAGGAGAGCC GCCTACCTTC TCACTGGCTC AAAAGTAAAG GGGCACACAC CACCATGGCA 
GATGCCCTCT GGCGCCTACG GGATTTAATG CTTCGAGACA CTCTCAACAT CCGACAGGCA 
TACAACCTAG AAAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346786.1
CDS	436..5196
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	NM_001359857.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359857.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGGA ATACTCATGA CAGTGAGATG TTGGGTCTCA GGAGGCTATC CAGTAAAAAG 
AACATAAGAA AACAGCGAAT GAAAATTTTA TTCAATGTTG TTCTTGAAGC TCGAGAGCCA 
GGTTCAGGCA GAAGACTTTG CGATCTATTT ATGGTTAAGC CATCCAAGAA GGACTATCCT 
GATTATTATA AAATCATCTT AGAGCCAATG GACCTGAAAA TAATTGAGCA TAACATCCGA 
AATGACAAAT ATGCAGGTGA AGAAGGAATG ATGGAAGACA TGAAACTCAT GTTCCGCAAT 
GCCAGGCACT ACAATGAGGA GGGCTCCCAG GTATACAATG ATGCCCATAT CCTGGAGAAG 
TTACTCAAAG ATAAAAGGAA AGAGCTGGGC CCTCTGCCTG ATGATGATGA CATGGCTTCT 
CCCAAACTTA AATTGAGTAG GAAGAGTGGT GTTTCTCCTA AGAAATCAAA GTACATGACT 
CCAATGCAGC AGAAACTGAA TGAAGTGTAT GAAGCTGTAA AGAACTATAC TGATAAGAGG 
GGTCGCCGCC TTAGTGCTAT ATTTCTAAGA CTCCCCTCTA GATCAGAGCT GCCTGACTAC 
TACCTGACCA TTAAAAAGCC CATGGACATG GAAAAAATTC GAAGTCACAT GATGGCAAAC 
AAGTACCAAG ACATAGATTC TATGGTAGAG GACTTTGTCA TGATGTTTAA TAATGCCTGT 
ACCTACAATG AACCAGAGTC TTTGATCTAC AAAGATGCCC TTGTACTGCA TAAAGTCCTC 
CTTGAGACTC GGAGAGACCT GGAGGGAGAT GAGGATTCTC ATGTCCCTAA TGTGACGTTG 
CTGATTCAAG AGCTCATCCA TAACCTTTTT GTGTCAGTCA TGAGTCATCA GGATGACGAA 
GGGAGGTGTT ACAGCGACTC CTTAGCAGAA ATTCCTGCTG TGGATCCCAA CTCTCCCAAT 
AAACCTCCCC TTACATTTGA CATTATCAGG AAAAATGTTG AAAGTAATCG GTATCGGCGA 
CTTGATTTAT TTCAGGAGCA TATGTTTGAA GTATTGGAAC GGGCAAGAAG GATGAACCGG 
ACAGATTCCG AAATATATGA GGATGCTGTA GAACTTCAGC AGTTTTTTAT TAGAATTCGT 
GATGAACTCT GCAAAAATGG AGAGATCCTT CTTTCTCCAG CACTCAGCTA TACCACAAAA 
CACTTGCATA ACGATGTGGA AAAAGAAAAA AAGGAAAAAT TGCCTAAAGA AATAGAGGAA 
GATAAACTAA AACGCGAAGA AGAAAAAAGA GAAGCTGAAA AAAGTGAAGA TTCCTCAGGT 
ACTACAGGCC TCTCAGGCTT ACATCGTACA TACAGCCAGG ACTGCAGCTT TAAGAACAGC 
ATGTATCATG TCGGAGATTA TGTCTATGTT GAACCTGCGG AGGCCAATCT ACAACCACAT 
ATAGTGTGTA TTGAGAGACT GTGGGAGGAT TCAGCTGGTG AAAAATGGTT GTACGGCTGT 
TGGTTTTATC GGCCAAATGA AACATTCCAT TTGGCTACAC GAAAATTTCT AGAAAAAGAA 
GTTTTTAAGA GTGACTACTA CAATAAAGTA CCTGTTAGTA AAATTCTAGG CAAATGTGTA 
GTCATGTTTG TCAAGGAATA CTTTAAATTA TGTCCAGAAA ACTTTCGCGA TGAGGATGTT 
TTTGTCTGTG AATCGAGGTA TTCTGCCAAA ACCAAATCTT TTAAGAAAAT TAAACTGTGG 
ACCATGCCCA TCAGTTCAGT TAGATTTGTC CCTCGGGATG TGCCTTTGCC TGTGGTCCGA 
GTGGCCTCTG TGTTTGCAAA TGCAGATAAA GGGGATGATG AGAAGAATAC AGACAACTCA 
GATGACAATA GAGCTGAAGA CAATTTTAAC TTGGAAAAGG AAAAAGAAGA TGTTCCTGTG 
GAGATGTCCA ATGGTGAGCC AGGTTGCCAC TACTTTGAGC AGCTTCGGTA CAATGACATG 
TGGCTGAAGG TTGGTGATTG TGTCTTCATC AAATCCCACG GCTTGGTGCG CCCTCGTGTG 
GGCAGAATTG AGAAAGTATG GGTCCGAGAT GGAGCTGCAT ATTTTTATGG CCCTATCTTC 
ATTCATCCAG AAGAAACAGA ACATGAGCCC ACAAAAATGT TCTACAAAAA AGAAGTGTTT 
CTGAGTAATC TGGAAGAGAC CTGCCCTATG AGTTGTATTC TGGGGAAATG TGCAGTGCTG 
TCATTCAAGG ACTTCCTCTC CTGCAGGCCA ACTGAAATAC CAGAAAATGA CATTCTGCTT 
TGTGAGAGCC GCTATAATGA GAGTGACAAG CAGATGAAGA AGTTCAAGGG TTTGAAGAGG 
TTTTCACTCT CTGCTAAAGT TGTAGATGAT GAAATCTACT ACTTCAGAAA ACCAATCATT 
CCTCAGAAGG AACCCTCACC TTTGTTAGAA AAGAAGATAC AATTGCTAGA AGCTAAATTT 
GCAGAGTTAG AAGGAGGAGA TGATGATATT GAGGAGATGG GAGAAGAGGA TAGTGAAGTC 
ATTGAAGCTC CATCTCTACC TCAACTGCAG ACACCCCTGG CCAATGAGTT GGACCTCATG 
CCCTATACAC CCCCACAGTC TACCCCAAAG TCTGCCAAAG GCAGTGCAAA GAAGGAAAGT 
TCTAAACGAA AAATCAACAT GAGTGGCTAC ATTTTGTTCA GCAGTGAAAT GAGAGCTGTG 
ATTAAAGCCC AGCACCCAGA CTACTCTTTT GGGGAGCTCA GCAGACTGGT GGGGACAGAA 
TGGAGAAACC TTGAAACAGC CAAGAAAGCA GAATATGAAG GTGTGATGAA TCAAGGAGTA 
GCCCCCATGG TAGGGACTCC AGCACCAGGT GGAAGTCCGT ATGGACAACA GGTAGGAGTT 
TTGGGACCTC CAGGGCAGCA GGCACCACCT CCATATCCTG GTCCTCATCC AGCTGGCCCC 
CCTGTCATAC AGCAGCCAAC AACGCCCATG TTTGTGGCTC CCCCACCAAA GACCCAAAGG 
CTTCTCCACT CAGAGGCCTA CCTGAAATAC ATTGAAGGAC TCAGTGCTGA ATCCAACAGC 
ATTAGCAAGT GGGACCAAAC TTTGGCAGCT CGAAGACGGG ATGTCCATTT GTCCAAAGAA 
CAGGAGAGCC GCCTACCTTC TCACTGGCTC AAAAGTAAAG GGGCACACAC CACCATGGCA 
GATGCCCTCT GGCGCCTACG GGATTTAATG CTTCGAGACA CTCTCAACAT CCGACAGGCA 
TACAACCTAG AAAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( NM_001359858.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346787.1 Mus musculus polybromo 1 (Pbrm1), transcript variant 6, mRNA.

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CloneID	OMu78836
Clone ID Related Accession (Same CDS sequence)	NM_001359857.1 , XM_017316160.1 , NM_001359858.1
Accession Version	NM_001359858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154727.2, AK166588.1, AK165182.1, AI615867.1, AK009582.1, CD803060.1, BG065752.2, CN707140.1, CN530699.1, CD803036.1, CN537785.1, BQ558912.1, AC154446.2 and AW048644.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.254103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGGA ATACTCATGA CAGTGAGATG TTGGGTCTCA GGAGGCTATC CAGTAAAAAG 
AACATAAGAA AACAGCGAAT GAAAATTTTA TTCAATGTTG TTCTTGAAGC TCGAGAGCCA 
GGTTCAGGCA GAAGACTTTG CGATCTATTT ATGGTTAAGC CATCCAAGAA GGACTATCCT 
GATTATTATA AAATCATCTT AGAGCCAATG GACCTGAAAA TAATTGAGCA TAACATCCGA 
AATGACAAAT ATGCAGGTGA AGAAGGAATG ATGGAAGACA TGAAACTCAT GTTCCGCAAT 
GCCAGGCACT ACAATGAGGA GGGCTCCCAG GTATACAATG ATGCCCATAT CCTGGAGAAG 
TTACTCAAAG ATAAAAGGAA AGAGCTGGGC CCTCTGCCTG ATGATGATGA CATGGCTTCT 
CCCAAACTTA AATTGAGTAG GAAGAGTGGT GTTTCTCCTA AGAAATCAAA GTACATGACT 
CCAATGCAGC AGAAACTGAA TGAAGTGTAT GAAGCTGTAA AGAACTATAC TGATAAGAGG 
GGTCGCCGCC TTAGTGCTAT ATTTCTAAGA CTCCCCTCTA GATCAGAGCT GCCTGACTAC 
TACCTGACCA TTAAAAAGCC CATGGACATG GAAAAAATTC GAAGTCACAT GATGGCAAAC 
AAGTACCAAG ACATAGATTC TATGGTAGAG GACTTTGTCA TGATGTTTAA TAATGCCTGT 
ACCTACAATG AACCAGAGTC TTTGATCTAC AAAGATGCCC TTGTACTGCA TAAAGTCCTC 
CTTGAGACTC GGAGAGACCT GGAGGGAGAT GAGGATTCTC ATGTCCCTAA TGTGACGTTG 
CTGATTCAAG AGCTCATCCA TAACCTTTTT GTGTCAGTCA TGAGTCATCA GGATGACGAA 
GGGAGGTGTT ACAGCGACTC CTTAGCAGAA ATTCCTGCTG TGGATCCCAA CTCTCCCAAT 
AAACCTCCCC TTACATTTGA CATTATCAGG AAAAATGTTG AAAGTAATCG GTATCGGCGA 
CTTGATTTAT TTCAGGAGCA TATGTTTGAA GTATTGGAAC GGGCAAGAAG GATGAACCGG 
ACAGATTCCG AAATATATGA GGATGCTGTA GAACTTCAGC AGTTTTTTAT TAGAATTCGT 
GATGAACTCT GCAAAAATGG AGAGATCCTT CTTTCTCCAG CACTCAGCTA TACCACAAAA 
CACTTGCATA ACGATGTGGA AAAAGAAAAA AAGGAAAAAT TGCCTAAAGA AATAGAGGAA 
GATAAACTAA AACGCGAAGA AGAAAAAAGA GAAGCTGAAA AAAGTGAAGA TTCCTCAGGT 
ACTACAGGCC TCTCAGGCTT ACATCGTACA TACAGCCAGG ACTGCAGCTT TAAGAACAGC 
ATGTATCATG TCGGAGATTA TGTCTATGTT GAACCTGCGG AGGCCAATCT ACAACCACAT 
ATAGTGTGTA TTGAGAGACT GTGGGAGGAT TCAGCTGGTG AAAAATGGTT GTACGGCTGT 
TGGTTTTATC GGCCAAATGA AACATTCCAT TTGGCTACAC GAAAATTTCT AGAAAAAGAA 
GTTTTTAAGA GTGACTACTA CAATAAAGTA CCTGTTAGTA AAATTCTAGG CAAATGTGTA 
GTCATGTTTG TCAAGGAATA CTTTAAATTA TGTCCAGAAA ACTTTCGCGA TGAGGATGTT 
TTTGTCTGTG AATCGAGGTA TTCTGCCAAA ACCAAATCTT TTAAGAAAAT TAAACTGTGG 
ACCATGCCCA TCAGTTCAGT TAGATTTGTC CCTCGGGATG TGCCTTTGCC TGTGGTCCGA 
GTGGCCTCTG TGTTTGCAAA TGCAGATAAA GGGGATGATG AGAAGAATAC AGACAACTCA 
GATGACAATA GAGCTGAAGA CAATTTTAAC TTGGAAAAGG AAAAAGAAGA TGTTCCTGTG 
GAGATGTCCA ATGGTGAGCC AGGTTGCCAC TACTTTGAGC AGCTTCGGTA CAATGACATG 
TGGCTGAAGG TTGGTGATTG TGTCTTCATC AAATCCCACG GCTTGGTGCG CCCTCGTGTG 
GGCAGAATTG AGAAAGTATG GGTCCGAGAT GGAGCTGCAT ATTTTTATGG CCCTATCTTC 
ATTCATCCAG AAGAAACAGA ACATGAGCCC ACAAAAATGT TCTACAAAAA AGAAGTGTTT 
CTGAGTAATC TGGAAGAGAC CTGCCCTATG AGTTGTATTC TGGGGAAATG TGCAGTGCTG 
TCATTCAAGG ACTTCCTCTC CTGCAGGCCA ACTGAAATAC CAGAAAATGA CATTCTGCTT 
TGTGAGAGCC GCTATAATGA GAGTGACAAG CAGATGAAGA AGTTCAAGGG TTTGAAGAGG 
TTTTCACTCT CTGCTAAAGT TGTAGATGAT GAAATCTACT ACTTCAGAAA ACCAATCATT 
CCTCAGAAGG AACCCTCACC TTTGTTAGAA AAGAAGATAC AATTGCTAGA AGCTAAATTT 
GCAGAGTTAG AAGGAGGAGA TGATGATATT GAGGAGATGG GAGAAGAGGA TAGTGAAGTC 
ATTGAAGCTC CATCTCTACC TCAACTGCAG ACACCCCTGG CCAATGAGTT GGACCTCATG 
CCCTATACAC CCCCACAGTC TACCCCAAAG TCTGCCAAAG GCAGTGCAAA GAAGGAAAGT 
TCTAAACGAA AAATCAACAT GAGTGGCTAC ATTTTGTTCA GCAGTGAAAT GAGAGCTGTG 
ATTAAAGCCC AGCACCCAGA CTACTCTTTT GGGGAGCTCA GCAGACTGGT GGGGACAGAA 
TGGAGAAACC TTGAAACAGC CAAGAAAGCA GAATATGAAG GTGTGATGAA TCAAGGAGTA 
GCCCCCATGG TAGGGACTCC AGCACCAGGT GGAAGTCCGT ATGGACAACA GGTAGGAGTT 
TTGGGACCTC CAGGGCAGCA GGCACCACCT CCATATCCTG GTCCTCATCC AGCTGGCCCC 
CCTGTCATAC AGCAGCCAAC AACGCCCATG TTTGTGGCTC CCCCACCAAA GACCCAAAGG 
CTTCTCCACT CAGAGGCCTA CCTGAAATAC ATTGAAGGAC TCAGTGCTGA ATCCAACAGC 
ATTAGCAAGT GGGACCAAAC TTTGGCAGCT CGAAGACGGG ATGTCCATTT GTCCAAAGAA 
CAGGAGAGCC GCCTACCTTC TCACTGGCTC AAAAGTAAAG GGGCACACAC CACCATGGCA 
GATGCCCTCT GGCGCCTACG GGATTTAATG CTTCGAGACA CTCTCAACAT CCGACAGGCA 
TACAACCTAG AAAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346787.1
CDS	330..5090
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	NM_001359858.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359858.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGGA ATACTCATGA CAGTGAGATG TTGGGTCTCA GGAGGCTATC CAGTAAAAAG 
AACATAAGAA AACAGCGAAT GAAAATTTTA TTCAATGTTG TTCTTGAAGC TCGAGAGCCA 
GGTTCAGGCA GAAGACTTTG CGATCTATTT ATGGTTAAGC CATCCAAGAA GGACTATCCT 
GATTATTATA AAATCATCTT AGAGCCAATG GACCTGAAAA TAATTGAGCA TAACATCCGA 
AATGACAAAT ATGCAGGTGA AGAAGGAATG ATGGAAGACA TGAAACTCAT GTTCCGCAAT 
GCCAGGCACT ACAATGAGGA GGGCTCCCAG GTATACAATG ATGCCCATAT CCTGGAGAAG 
TTACTCAAAG ATAAAAGGAA AGAGCTGGGC CCTCTGCCTG ATGATGATGA CATGGCTTCT 
CCCAAACTTA AATTGAGTAG GAAGAGTGGT GTTTCTCCTA AGAAATCAAA GTACATGACT 
CCAATGCAGC AGAAACTGAA TGAAGTGTAT GAAGCTGTAA AGAACTATAC TGATAAGAGG 
GGTCGCCGCC TTAGTGCTAT ATTTCTAAGA CTCCCCTCTA GATCAGAGCT GCCTGACTAC 
TACCTGACCA TTAAAAAGCC CATGGACATG GAAAAAATTC GAAGTCACAT GATGGCAAAC 
AAGTACCAAG ACATAGATTC TATGGTAGAG GACTTTGTCA TGATGTTTAA TAATGCCTGT 
ACCTACAATG AACCAGAGTC TTTGATCTAC AAAGATGCCC TTGTACTGCA TAAAGTCCTC 
CTTGAGACTC GGAGAGACCT GGAGGGAGAT GAGGATTCTC ATGTCCCTAA TGTGACGTTG 
CTGATTCAAG AGCTCATCCA TAACCTTTTT GTGTCAGTCA TGAGTCATCA GGATGACGAA 
GGGAGGTGTT ACAGCGACTC CTTAGCAGAA ATTCCTGCTG TGGATCCCAA CTCTCCCAAT 
AAACCTCCCC TTACATTTGA CATTATCAGG AAAAATGTTG AAAGTAATCG GTATCGGCGA 
CTTGATTTAT TTCAGGAGCA TATGTTTGAA GTATTGGAAC GGGCAAGAAG GATGAACCGG 
ACAGATTCCG AAATATATGA GGATGCTGTA GAACTTCAGC AGTTTTTTAT TAGAATTCGT 
GATGAACTCT GCAAAAATGG AGAGATCCTT CTTTCTCCAG CACTCAGCTA TACCACAAAA 
CACTTGCATA ACGATGTGGA AAAAGAAAAA AAGGAAAAAT TGCCTAAAGA AATAGAGGAA 
GATAAACTAA AACGCGAAGA AGAAAAAAGA GAAGCTGAAA AAAGTGAAGA TTCCTCAGGT 
ACTACAGGCC TCTCAGGCTT ACATCGTACA TACAGCCAGG ACTGCAGCTT TAAGAACAGC 
ATGTATCATG TCGGAGATTA TGTCTATGTT GAACCTGCGG AGGCCAATCT ACAACCACAT 
ATAGTGTGTA TTGAGAGACT GTGGGAGGAT TCAGCTGGTG AAAAATGGTT GTACGGCTGT 
TGGTTTTATC GGCCAAATGA AACATTCCAT TTGGCTACAC GAAAATTTCT AGAAAAAGAA 
GTTTTTAAGA GTGACTACTA CAATAAAGTA CCTGTTAGTA AAATTCTAGG CAAATGTGTA 
GTCATGTTTG TCAAGGAATA CTTTAAATTA TGTCCAGAAA ACTTTCGCGA TGAGGATGTT 
TTTGTCTGTG AATCGAGGTA TTCTGCCAAA ACCAAATCTT TTAAGAAAAT TAAACTGTGG 
ACCATGCCCA TCAGTTCAGT TAGATTTGTC CCTCGGGATG TGCCTTTGCC TGTGGTCCGA 
GTGGCCTCTG TGTTTGCAAA TGCAGATAAA GGGGATGATG AGAAGAATAC AGACAACTCA 
GATGACAATA GAGCTGAAGA CAATTTTAAC TTGGAAAAGG AAAAAGAAGA TGTTCCTGTG 
GAGATGTCCA ATGGTGAGCC AGGTTGCCAC TACTTTGAGC AGCTTCGGTA CAATGACATG 
TGGCTGAAGG TTGGTGATTG TGTCTTCATC AAATCCCACG GCTTGGTGCG CCCTCGTGTG 
GGCAGAATTG AGAAAGTATG GGTCCGAGAT GGAGCTGCAT ATTTTTATGG CCCTATCTTC 
ATTCATCCAG AAGAAACAGA ACATGAGCCC ACAAAAATGT TCTACAAAAA AGAAGTGTTT 
CTGAGTAATC TGGAAGAGAC CTGCCCTATG AGTTGTATTC TGGGGAAATG TGCAGTGCTG 
TCATTCAAGG ACTTCCTCTC CTGCAGGCCA ACTGAAATAC CAGAAAATGA CATTCTGCTT 
TGTGAGAGCC GCTATAATGA GAGTGACAAG CAGATGAAGA AGTTCAAGGG TTTGAAGAGG 
TTTTCACTCT CTGCTAAAGT TGTAGATGAT GAAATCTACT ACTTCAGAAA ACCAATCATT 
CCTCAGAAGG AACCCTCACC TTTGTTAGAA AAGAAGATAC AATTGCTAGA AGCTAAATTT 
GCAGAGTTAG AAGGAGGAGA TGATGATATT GAGGAGATGG GAGAAGAGGA TAGTGAAGTC 
ATTGAAGCTC CATCTCTACC TCAACTGCAG ACACCCCTGG CCAATGAGTT GGACCTCATG 
CCCTATACAC CCCCACAGTC TACCCCAAAG TCTGCCAAAG GCAGTGCAAA GAAGGAAAGT 
TCTAAACGAA AAATCAACAT GAGTGGCTAC ATTTTGTTCA GCAGTGAAAT GAGAGCTGTG 
ATTAAAGCCC AGCACCCAGA CTACTCTTTT GGGGAGCTCA GCAGACTGGT GGGGACAGAA 
TGGAGAAACC TTGAAACAGC CAAGAAAGCA GAATATGAAG GTGTGATGAA TCAAGGAGTA 
GCCCCCATGG TAGGGACTCC AGCACCAGGT GGAAGTCCGT ATGGACAACA GGTAGGAGTT 
TTGGGACCTC CAGGGCAGCA GGCACCACCT CCATATCCTG GTCCTCATCC AGCTGGCCCC 
CCTGTCATAC AGCAGCCAAC AACGCCCATG TTTGTGGCTC CCCCACCAAA GACCCAAAGG 
CTTCTCCACT CAGAGGCCTA CCTGAAATAC ATTGAAGGAC TCAGTGCTGA ATCCAACAGC 
ATTAGCAAGT GGGACCAAAC TTTGGCAGCT CGAAGACGGG ATGTCCATTT GTCCAAAGAA 
CAGGAGAGCC GCCTACCTTC TCACTGGCTC AAAAGTAAAG GGGCACACAC CACCATGGCA 
GATGCCCTCT GGCGCCTACG GGATTTAATG CTTCGAGACA CTCTCAACAT CCGACAGGCA 
TACAACCTAG AAAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( NM_001359855.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346784.1 Mus musculus polybromo 1 (Pbrm1), transcript variant 3, mRNA.

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CloneID	OMu84287
Clone ID Related Accession (Same CDS sequence)	NM_001359855.1
Accession Version	NM_001359855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154727.2, AK166588.1, AK165182.1, AI615867.1, CA557251.1, AI507524.1, CD803060.1, BG065752.2, CN707140.1, CN530699.1, CD803036.1, BC055708.1, AC154446.2 and AW048644.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660825.144636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGGCA TGATGGGTGG CTATCCGCCA GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT 
GGCCTTGTTA GCATGGGCAG CATGCAGCCA CTTCACCCTG GGGGGCCTCC ACCTCACCAT 
CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC ATCCCACCAC CGGGTGTGAT GAATCAAGGA 
GTAGCCCCCA TGGTAGGGAC TCCAGCACCA GGTGGAAGTC CGTATGGACA ACAGGTAGGA 
GTTTTGGGAC CTCCAGGGCA GCAGGCACCA CCTCCATATC CTGGTCCTCA TCCAGCTGGC 
CCCCCTGTCA TACAGCAGCC AACAACGCCC ATGTTTGTGG CTCCCCCACC AAAGACCCAA 
AGGCTTCTCC ACTCAGAGGC CTACCTGAAA TACATTGAAG GACTCAGTGC TGAATCCAAC 
AGCATTAGCA AGTGGGACCA AACTTTGGCA GCTCGAAGAC GGGATGTCCA TTTGTCCAAA 
GAACAGGAGA GCCGCCTACC TTCTCACTGG CTCAAAAGTA AAGGGGCACA CACCACCATG 
GCAGATGCCC TCTGGCGCCT ACGGGATTTA ATGCTTCGAG ACACTCTCAA CATCCGACAG 
GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346784.1
CDS	330..5333
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	NM_001359855.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359855.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGGCA TGATGGGTGG CTATCCGCCA GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT 
GGCCTTGTTA GCATGGGCAG CATGCAGCCA CTTCACCCTG GGGGGCCTCC ACCTCACCAT 
CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC ATCCCACCAC CGGGTGTGAT GAATCAAGGA 
GTAGCCCCCA TGGTAGGGAC TCCAGCACCA GGTGGAAGTC CGTATGGACA ACAGGTAGGA 
GTTTTGGGAC CTCCAGGGCA GCAGGCACCA CCTCCATATC CTGGTCCTCA TCCAGCTGGC 
CCCCCTGTCA TACAGCAGCC AACAACGCCC ATGTTTGTGG CTCCCCCACC AAAGACCCAA 
AGGCTTCTCC ACTCAGAGGC CTACCTGAAA TACATTGAAG GACTCAGTGC TGAATCCAAC 
AGCATTAGCA AGTGGGACCA AACTTTGGCA GCTCGAAGAC GGGATGTCCA TTTGTCCAAA 
GAACAGGAGA GCCGCCTACC TTCTCACTGG CTCAAAAGTA AAGGGGCACA CACCACCATG 
GCAGATGCCC TCTGGCGCCT ACGGGATTTA ATGCTTCGAG ACACTCTCAA CATCCGACAG 
GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316145.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171634.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu78826
Clone ID Related Accession (Same CDS sequence)	XM_017316145.1
Accession Version	XM_017316145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GTAAAAAGAA CATAAGAAAA 
CAGCGAATGA AAATTTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGCG ATCTATTTAT GGTTAAGCCA TCCAAGAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTAG AGCCAATGGA CCTGAAAATA ATTGAGCATA ACATCCGAAA TGACAAATAT 
GCAGGTGAAG AAGGAATGAT GGAAGACATG AAACTCATGT TCCGCAATGC CAGGCACTAC 
AATGAGGAGG GCTCCCAGGT ATACAATGAT GCCCATATCC TGGAGAAGTT ACTCAAAGAT 
AAAAGGAAAG AGCTGGGCCC TCTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTTAAA 
TTGAGTAGGA AGAGTGGTGT TTCTCCTAAG AAATCAAAGT ACATGACTCC AATGCAGCAG 
AAACTGAATG AAGTGTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTT 
AGTGCTATAT TTCTAAGACT CCCCTCTAGA TCAGAGCTGC CTGACTACTA CCTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCAAACAA GTACCAAGAC 
ATAGATTCTA TGGTAGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC CTACAATGAA 
CCAGAGTCTT TGATCTACAA AGATGCCCTT GTACTGCATA AAGTCCTCCT TGAGACTCGG 
AGAGACCTGG AGGGAGATGA GGATTCTCAT GTCCCTAATG TGACGTTGCT GATTCAAGAG 
CTCATCCATA ACCTTTTTGT GTCAGTCATG AGTCATCAGG ATGACGAAGG GAGGTGTTAC 
AGCGACTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAACT CTCCCAATAA ACCTCCCCTT 
ACATTTGACA TTATCAGGAA AAATGTTGAA AGTAATCGGT ATCGGCGACT TGATTTATTT 
CAGGAGCATA TGTTTGAAGT ATTGGAACGG GCAAGAAGGA TGAACCGGAC AGATTCCGAA 
ATATATGAGG ATGCTGTAGA ACTTCAGCAG TTTTTTATTA GAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCTCCAGCA CTCAGCTATA CCACAAAACA CTTGCATAAC 
GATGTGGAAA AAGAAAAAAA GGAAAAATTG CCTAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGCGAAGAAG AAAAAAGAGA AGCTGAAAAA AGTGAAGATT CCTCAGGTAC TACAGGCCTC 
TCAGGCTTAC ATCGTACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTATCATGTC 
GGAGATTATG TCTATGTTGA ACCTGCGGAG GCCAATCTAC AACCACATAT AGTGTGTATT 
GAGAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ACGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTACTACA ATAAAGTACC TGTTAGTAAA ATTCTAGGCA AATGTGTAGT CATGTTTGTC 
AAGGAATACT TTAAATTATG TCCAGAAAAC TTTCGCGATG AGGATGTTTT TGTCTGTGAA 
TCGAGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGTTCAGTTA GATTTGTCCC TCGGGATGTG CCTTTGCCTG TGGTCCGAGT GGCCTCTGTG 
TTTGCAAATG CAGATAAAGG GGATGATGAG AAGAATACAG ACAACTCAGA TGACAATAGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TTCCTGTGGA GATGTCCAAT 
GGTGAGCCAG GTTGCCACTA CTTTGAGCAG CTTCGGTACA ATGACATGTG GCTGAAGGTT 
GGTGATTGTG TCTTCATCAA ATCCCACGGC TTGGTGCGCC CTCGTGTGGG CAGAATTGAG 
AAAGTATGGG TCCGAGATGG AGCTGCATAT TTTTATGGCC CTATCTTCAT TCATCCAGAA 
GAAACAGAAC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTGTTTCT GAGTAATCTG 
GAAGAGACCT GCCCTATGAG TTGTATTCTG GGGAAATGTG CAGTGCTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TATAATGAGA GTGACAAGCA GATGAAGAAG TTCAAGGGTT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTTG TAGATGATGA AATCTACTAC TTCAGAAAAC CAATCATTCC TCAGAAGGAA 
CCCTCACCTT TGTTAGAAAA GAAGATACAA TTGCTAGAAG CTAAATTTGC AGAGTTAGAA 
GGAGGAGATG ATGATATTGA GGAGATGGGA GAAGAGGATA GTGAAGTCAT TGAAGCTCCA 
TCTCTACCTC AACTGCAGAC ACCCCTGGCC AATGAGTTGG ACCTCATGCC CTATACACCC 
CCACAGCTTT CTGTCTTACA GTCTACCCCA AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA 
AGTTCTAAAC GAAAAATCAA CATGAGTGGC TACATTTTGT TCAGCAGTGA AATGAGAGCT 
GTGATTAAAG CCCAGCACCC AGACTACTCT TTTGGGGAGC TCAGCAGACT GGTGGGGACA 
GAATGGAGAA ACCTTGAAAC AGCCAAGAAA GCAGAATATG AAGAGCGGGC AGCTAAAGTT 
GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA CAGCAACAGC AGCCGAGTGC TTCTCCCCGA 
GCAGGCACCC CTGTGGGGGC TCTCATGGGG GTGGTGCCAC CACCAACACC AATGGGGATG 
CTCAATCAGC AGTTGACACC TGTTGCAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCTCACCA TCTTCCGCCA GGTGTGCCTG GCCTCCCAGG CATCCCACCA 
CCGGGTGTGA TGAATCAAGG AGTAGCCCCC ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AACAGGTAGG AGTTTTGGGA CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGTCCTC ATCCAGCTGG CCCCCCTGTC ATACAGCAGC CAACAACGCC CATGTTTGTG 
GCTCCCCCAC CAAAGACCCA AAGGCTTCTC CACTCAGAGG CCTACCTGAA ATACATTGAA 
GGACTCAGTG CTGAATCCAA CAGCATTAGC AAGTGGGACC AAACTTTGGC AGCTCGAAGA 
CGGGATGTCC ATTTGTCCAA AGAACAGGAG AGCCGCCTAC CTTCTCACTG GCTCAAAAGT 
AAAGGGGCAC ACACCACCAT GGCAGATGCC CTCTGGCGCC TACGGGATTT AATGCTTCGA 
GACACTCTCA ACATCCGACA GGCATACAAC CTAGAAAATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171634.1
CDS	359..5503
Translation	MRRLAFRGAGCALVKLKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316145.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316145.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GTAAAAAGAA CATAAGAAAA 
CAGCGAATGA AAATTTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGCG ATCTATTTAT GGTTAAGCCA TCCAAGAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTAG AGCCAATGGA CCTGAAAATA ATTGAGCATA ACATCCGAAA TGACAAATAT 
GCAGGTGAAG AAGGAATGAT GGAAGACATG AAACTCATGT TCCGCAATGC CAGGCACTAC 
AATGAGGAGG GCTCCCAGGT ATACAATGAT GCCCATATCC TGGAGAAGTT ACTCAAAGAT 
AAAAGGAAAG AGCTGGGCCC TCTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTTAAA 
TTGAGTAGGA AGAGTGGTGT TTCTCCTAAG AAATCAAAGT ACATGACTCC AATGCAGCAG 
AAACTGAATG AAGTGTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTT 
AGTGCTATAT TTCTAAGACT CCCCTCTAGA TCAGAGCTGC CTGACTACTA CCTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCAAACAA GTACCAAGAC 
ATAGATTCTA TGGTAGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC CTACAATGAA 
CCAGAGTCTT TGATCTACAA AGATGCCCTT GTACTGCATA AAGTCCTCCT TGAGACTCGG 
AGAGACCTGG AGGGAGATGA GGATTCTCAT GTCCCTAATG TGACGTTGCT GATTCAAGAG 
CTCATCCATA ACCTTTTTGT GTCAGTCATG AGTCATCAGG ATGACGAAGG GAGGTGTTAC 
AGCGACTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAACT CTCCCAATAA ACCTCCCCTT 
ACATTTGACA TTATCAGGAA AAATGTTGAA AGTAATCGGT ATCGGCGACT TGATTTATTT 
CAGGAGCATA TGTTTGAAGT ATTGGAACGG GCAAGAAGGA TGAACCGGAC AGATTCCGAA 
ATATATGAGG ATGCTGTAGA ACTTCAGCAG TTTTTTATTA GAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCTCCAGCA CTCAGCTATA CCACAAAACA CTTGCATAAC 
GATGTGGAAA AAGAAAAAAA GGAAAAATTG CCTAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGCGAAGAAG AAAAAAGAGA AGCTGAAAAA AGTGAAGATT CCTCAGGTAC TACAGGCCTC 
TCAGGCTTAC ATCGTACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTATCATGTC 
GGAGATTATG TCTATGTTGA ACCTGCGGAG GCCAATCTAC AACCACATAT AGTGTGTATT 
GAGAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ACGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTACTACA ATAAAGTACC TGTTAGTAAA ATTCTAGGCA AATGTGTAGT CATGTTTGTC 
AAGGAATACT TTAAATTATG TCCAGAAAAC TTTCGCGATG AGGATGTTTT TGTCTGTGAA 
TCGAGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGTTCAGTTA GATTTGTCCC TCGGGATGTG CCTTTGCCTG TGGTCCGAGT GGCCTCTGTG 
TTTGCAAATG CAGATAAAGG GGATGATGAG AAGAATACAG ACAACTCAGA TGACAATAGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TTCCTGTGGA GATGTCCAAT 
GGTGAGCCAG GTTGCCACTA CTTTGAGCAG CTTCGGTACA ATGACATGTG GCTGAAGGTT 
GGTGATTGTG TCTTCATCAA ATCCCACGGC TTGGTGCGCC CTCGTGTGGG CAGAATTGAG 
AAAGTATGGG TCCGAGATGG AGCTGCATAT TTTTATGGCC CTATCTTCAT TCATCCAGAA 
GAAACAGAAC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTGTTTCT GAGTAATCTG 
GAAGAGACCT GCCCTATGAG TTGTATTCTG GGGAAATGTG CAGTGCTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TATAATGAGA GTGACAAGCA GATGAAGAAG TTCAAGGGTT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTTG TAGATGATGA AATCTACTAC TTCAGAAAAC CAATCATTCC TCAGAAGGAA 
CCCTCACCTT TGTTAGAAAA GAAGATACAA TTGCTAGAAG CTAAATTTGC AGAGTTAGAA 
GGAGGAGATG ATGATATTGA GGAGATGGGA GAAGAGGATA GTGAAGTCAT TGAAGCTCCA 
TCTCTACCTC AACTGCAGAC ACCCCTGGCC AATGAGTTGG ACCTCATGCC CTATACACCC 
CCACAGCTTT CTGTCTTACA GTCTACCCCA AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA 
AGTTCTAAAC GAAAAATCAA CATGAGTGGC TACATTTTGT TCAGCAGTGA AATGAGAGCT 
GTGATTAAAG CCCAGCACCC AGACTACTCT TTTGGGGAGC TCAGCAGACT GGTGGGGACA 
GAATGGAGAA ACCTTGAAAC AGCCAAGAAA GCAGAATATG AAGAGCGGGC AGCTAAAGTT 
GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA CAGCAACAGC AGCCGAGTGC TTCTCCCCGA 
GCAGGCACCC CTGTGGGGGC TCTCATGGGG GTGGTGCCAC CACCAACACC AATGGGGATG 
CTCAATCAGC AGTTGACACC TGTTGCAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCTCACCA TCTTCCGCCA GGTGTGCCTG GCCTCCCAGG CATCCCACCA 
CCGGGTGTGA TGAATCAAGG AGTAGCCCCC ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AACAGGTAGG AGTTTTGGGA CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGTCCTC ATCCAGCTGG CCCCCCTGTC ATACAGCAGC CAACAACGCC CATGTTTGTG 
GCTCCCCCAC CAAAGACCCA AAGGCTTCTC CACTCAGAGG CCTACCTGAA ATACATTGAA 
GGACTCAGTG CTGAATCCAA CAGCATTAGC AAGTGGGACC AAACTTTGGC AGCTCGAAGA 
CGGGATGTCC ATTTGTCCAA AGAACAGGAG AGCCGCCTAC CTTCTCACTG GCTCAAAAGT 
AAAGGGGCAC ACACCACCAT GGCAGATGCC CTCTGGCGCC TACGGGATTT AATGCTTCGA 
GACACTCTCA ACATCCGACA GGCATACAAC CTAGAAAATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316148.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171637.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OMu78827
Clone ID Related Accession (Same CDS sequence)	XM_017316148.1 , XM_017316149.1
Accession Version	XM_017316148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGGAATA CTCATGACAG TGAGATGTTG GGTCTCAGGA GGCTATCCAG TAAAAAGAAC 
ATAAGAAAAC AGCGAATGAA AATTTTATTC AATGTTGTTC TTGAAGCTCG AGAGCCAGGT 
TCAGGCAGAA GACTTTGCGA TCTATTTATG GTTAAGCCAT CCAAGAAGGA CTATCCTGAT 
TATTATAAAA TCATCTTAGA GCCAATGGAC CTGAAAATAA TTGAGCATAA CATCCGAAAT 
GACAAATATG CAGGTGAAGA AGGAATGATG GAAGACATGA AACTCATGTT CCGCAATGCC 
AGGCACTACA ATGAGGAGGG CTCCCAGGTA TACAATGATG CCCATATCCT GGAGAAGTTA 
CTCAAAGATA AAAGGAAAGA GCTGGGCCCT CTGCCTGATG ATGATGACAT GGCTTCTCCC 
AAACTTAAAT TGAGTAGGAA GAGTGGTGTT TCTCCTAAGA AATCAAAGTA CATGACTCCA 
ATGCAGCAGA AACTGAATGA AGTGTATGAA GCTGTAAAGA ACTATACTGA TAAGAGGGGT 
CGCCGCCTTA GTGCTATATT TCTAAGACTC CCCTCTAGAT CAGAGCTGCC TGACTACTAC 
CTGACCATTA AAAAGCCCAT GGACATGGAA AAAATTCGAA GTCACATGAT GGCAAACAAG 
TACCAAGACA TAGATTCTAT GGTAGAGGAC TTTGTCATGA TGTTTAATAA TGCCTGTACC 
TACAATGAAC CAGAGTCTTT GATCTACAAA GATGCCCTTG TACTGCATAA AGTCCTCCTT 
GAGACTCGGA GAGACCTGGA GGGAGATGAG GATTCTCATG TCCCTAATGT GACGTTGCTG 
ATTCAAGAGC TCATCCATAA CCTTTTTGTG TCAGTCATGA GTCATCAGGA TGACGAAGGG 
AGGTGTTACA GCGACTCCTT AGCAGAAATT CCTGCTGTGG ATCCCAACTC TCCCAATAAA 
CCTCCCCTTA CATTTGACAT TATCAGGAAA AATGTTGAAA GTAATCGGTA TCGGCGACTT 
GATTTATTTC AGGAGCATAT GTTTGAAGTA TTGGAACGGG CAAGAAGGAT GAACCGGACA 
GATTCCGAAA TATATGAGGA TGCTGTAGAA CTTCAGCAGT TTTTTATTAG AATTCGTGAT 
GAACTCTGCA AAAATGGAGA GATCCTTCTT TCTCCAGCAC TCAGCTATAC CACAAAACAC 
TTGCATAACG ATGTGGAAAA AGAAAAAAAG GAAAAATTGC CTAAAGAAAT AGAGGAAGAT 
AAACTAAAAC GCGAAGAAGA AAAAAGAGAA GCTGAAAAAA GTGAAGATTC CTCAGGTACT 
ACAGGCCTCT CAGGCTTACA TCGTACATAC AGCCAGGACT GCAGCTTTAA GAACAGCATG 
TATCATGTCG GAGATTATGT CTATGTTGAA CCTGCGGAGG CCAATCTACA ACCACATATA 
GTGTGTATTG AGAGACTGTG GGAGGATTCA GCTGGTGAAA AATGGTTGTA CGGCTGTTGG 
TTTTATCGGC CAAATGAAAC ATTCCATTTG GCTACACGAA AATTTCTAGA AAAAGAAGTT 
TTTAAGAGTG ACTACTACAA TAAAGTACCT GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC 
ATGTTTGTCA AGGAATACTT TAAATTATGT CCAGAAAACT TTCGCGATGA GGATGTTTTT 
GTCTGTGAAT CGAGGTATTC TGCCAAAACC AAATCTTTTA AGAAAATTAA ACTGTGGACC 
ATGCCCATCA GTTCAGTTAG ATTTGTCCCT CGGGATGTGC CTTTGCCTGT GGTCCGAGTG 
GCCTCTGTGT TTGCAAATGC AGATAAAGGG GATGATGAGA AGAATACAGA CAACTCAGAT 
GACAATAGAG CTGAAGACAA TTTTAACTTG GAAAAGGAAA AAGAAGATGT TCCTGTGGAG 
ATGTCCAATG GTGAGCCAGG TTGCCACTAC TTTGAGCAGC TTCGGTACAA TGACATGTGG 
CTGAAGGTTG GTGATTGTGT CTTCATCAAA TCCCACGGCT TGGTGCGCCC TCGTGTGGGC 
AGAATTGAGA AAGTATGGGT CCGAGATGGA GCTGCATATT TTTATGGCCC TATCTTCATT 
CATCCAGAAG AAACAGAACA TGAGCCCACA AAAATGTTCT ACAAAAAAGA AGTGTTTCTG 
AGTAATCTGG AAGAGACCTG CCCTATGAGT TGTATTCTGG GGAAATGTGC AGTGCTGTCA 
TTCAAGGACT TCCTCTCCTG CAGGCCAACT GAAATACCAG AAAATGACAT TCTGCTTTGT 
GAGAGCCGCT ATAATGAGAG TGACAAGCAG ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT 
TCACTCTCTG CTAAAGTTGT AGATGATGAA ATCTACTACT TCAGAAAACC AATCATTCCT 
CAGAAGGAAC CCTCACCTTT GTTAGAAAAG AAGATACAAT TGCTAGAAGC TAAATTTGCA 
GAGTTAGAAG GAGGAGATGA TGATATTGAG GAGATGGGAG AAGAGGATAG TGAAGTCATT 
GAAGCTCCAT CTCTACCTCA ACTGCAGACA CCCCTGGCCA ATGAGTTGGA CCTCATGCCC 
TATACACCCC CACAGCTTTC TGTCTTACAG TCTACCCCAA AGTCTGCCAA AGGCAGTGCA 
AAGAAGGAAA GTTCTAAACG AAAAATCAAC ATGAGTGGCT ACATTTTGTT CAGCAGTGAA 
ATGAGAGCTG TGATTAAAGC CCAGCACCCA GACTACTCTT TTGGGGAGCT CAGCAGACTG 
GTGGGGACAG AATGGAGAAA CCTTGAAACA GCCAAGAAAG CAGAATATGA AGAGCGGGCA 
GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG CGAGCAGCAC AGCAACAGCA GCCGAGTGCT 
TCTCCCCGAG CAGGCACCCC TGTGGGGGCT CTCATGGGGG TGGTGCCACC ACCAACACCA 
ATGGGGATGC TCAATCAGCA GTTGACACCT GTTGCAGGCA TGATGGGTGG CTATCCGCCA 
GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT GGCCTTGTTA GCATGGGCAG CATGCAGCCA 
CTTCACCCTG GGGGGCCTCC ACCTCACCAT CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC 
ATCCCACCAC CGGGTGTGAT GAATCAAGGA GTAGCCCCCA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CGTATGGACA ACAGGTAGGA GTTTTGGGAC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CTGGTCCTCA TCCAGCTGGC CCCCCTGTCA TACAGCAGCC AACAACGCCC 
ATGTTTGTGG CTCCCCCACC AAAGACCCAA AGGCTTCTCC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC TGAATCCAAC AGCATTAGCA AGTGGGACCA AACTTTGGCA 
GCTCGAAGAC GGGATGTCCA TTTGTCCAAA GAACAGGAGA GCCGCCTACC TTCTCACTGG 
CTCAAAAGTA AAGGGGCACA CACCACCATG GCAGATGCCC TCTGGCGCCT ACGGGATTTA 
ATGCTTCGAG ACACTCTCAA CATCCGACAG GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171637.1
CDS	359..5452
Translation	MRRLAFRGAGCALVKLKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316148.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316148.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGGAATA CTCATGACAG TGAGATGTTG GGTCTCAGGA GGCTATCCAG TAAAAAGAAC 
ATAAGAAAAC AGCGAATGAA AATTTTATTC AATGTTGTTC TTGAAGCTCG AGAGCCAGGT 
TCAGGCAGAA GACTTTGCGA TCTATTTATG GTTAAGCCAT CCAAGAAGGA CTATCCTGAT 
TATTATAAAA TCATCTTAGA GCCAATGGAC CTGAAAATAA TTGAGCATAA CATCCGAAAT 
GACAAATATG CAGGTGAAGA AGGAATGATG GAAGACATGA AACTCATGTT CCGCAATGCC 
AGGCACTACA ATGAGGAGGG CTCCCAGGTA TACAATGATG CCCATATCCT GGAGAAGTTA 
CTCAAAGATA AAAGGAAAGA GCTGGGCCCT CTGCCTGATG ATGATGACAT GGCTTCTCCC 
AAACTTAAAT TGAGTAGGAA GAGTGGTGTT TCTCCTAAGA AATCAAAGTA CATGACTCCA 
ATGCAGCAGA AACTGAATGA AGTGTATGAA GCTGTAAAGA ACTATACTGA TAAGAGGGGT 
CGCCGCCTTA GTGCTATATT TCTAAGACTC CCCTCTAGAT CAGAGCTGCC TGACTACTAC 
CTGACCATTA AAAAGCCCAT GGACATGGAA AAAATTCGAA GTCACATGAT GGCAAACAAG 
TACCAAGACA TAGATTCTAT GGTAGAGGAC TTTGTCATGA TGTTTAATAA TGCCTGTACC 
TACAATGAAC CAGAGTCTTT GATCTACAAA GATGCCCTTG TACTGCATAA AGTCCTCCTT 
GAGACTCGGA GAGACCTGGA GGGAGATGAG GATTCTCATG TCCCTAATGT GACGTTGCTG 
ATTCAAGAGC TCATCCATAA CCTTTTTGTG TCAGTCATGA GTCATCAGGA TGACGAAGGG 
AGGTGTTACA GCGACTCCTT AGCAGAAATT CCTGCTGTGG ATCCCAACTC TCCCAATAAA 
CCTCCCCTTA CATTTGACAT TATCAGGAAA AATGTTGAAA GTAATCGGTA TCGGCGACTT 
GATTTATTTC AGGAGCATAT GTTTGAAGTA TTGGAACGGG CAAGAAGGAT GAACCGGACA 
GATTCCGAAA TATATGAGGA TGCTGTAGAA CTTCAGCAGT TTTTTATTAG AATTCGTGAT 
GAACTCTGCA AAAATGGAGA GATCCTTCTT TCTCCAGCAC TCAGCTATAC CACAAAACAC 
TTGCATAACG ATGTGGAAAA AGAAAAAAAG GAAAAATTGC CTAAAGAAAT AGAGGAAGAT 
AAACTAAAAC GCGAAGAAGA AAAAAGAGAA GCTGAAAAAA GTGAAGATTC CTCAGGTACT 
ACAGGCCTCT CAGGCTTACA TCGTACATAC AGCCAGGACT GCAGCTTTAA GAACAGCATG 
TATCATGTCG GAGATTATGT CTATGTTGAA CCTGCGGAGG CCAATCTACA ACCACATATA 
GTGTGTATTG AGAGACTGTG GGAGGATTCA GCTGGTGAAA AATGGTTGTA CGGCTGTTGG 
TTTTATCGGC CAAATGAAAC ATTCCATTTG GCTACACGAA AATTTCTAGA AAAAGAAGTT 
TTTAAGAGTG ACTACTACAA TAAAGTACCT GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC 
ATGTTTGTCA AGGAATACTT TAAATTATGT CCAGAAAACT TTCGCGATGA GGATGTTTTT 
GTCTGTGAAT CGAGGTATTC TGCCAAAACC AAATCTTTTA AGAAAATTAA ACTGTGGACC 
ATGCCCATCA GTTCAGTTAG ATTTGTCCCT CGGGATGTGC CTTTGCCTGT GGTCCGAGTG 
GCCTCTGTGT TTGCAAATGC AGATAAAGGG GATGATGAGA AGAATACAGA CAACTCAGAT 
GACAATAGAG CTGAAGACAA TTTTAACTTG GAAAAGGAAA AAGAAGATGT TCCTGTGGAG 
ATGTCCAATG GTGAGCCAGG TTGCCACTAC TTTGAGCAGC TTCGGTACAA TGACATGTGG 
CTGAAGGTTG GTGATTGTGT CTTCATCAAA TCCCACGGCT TGGTGCGCCC TCGTGTGGGC 
AGAATTGAGA AAGTATGGGT CCGAGATGGA GCTGCATATT TTTATGGCCC TATCTTCATT 
CATCCAGAAG AAACAGAACA TGAGCCCACA AAAATGTTCT ACAAAAAAGA AGTGTTTCTG 
AGTAATCTGG AAGAGACCTG CCCTATGAGT TGTATTCTGG GGAAATGTGC AGTGCTGTCA 
TTCAAGGACT TCCTCTCCTG CAGGCCAACT GAAATACCAG AAAATGACAT TCTGCTTTGT 
GAGAGCCGCT ATAATGAGAG TGACAAGCAG ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT 
TCACTCTCTG CTAAAGTTGT AGATGATGAA ATCTACTACT TCAGAAAACC AATCATTCCT 
CAGAAGGAAC CCTCACCTTT GTTAGAAAAG AAGATACAAT TGCTAGAAGC TAAATTTGCA 
GAGTTAGAAG GAGGAGATGA TGATATTGAG GAGATGGGAG AAGAGGATAG TGAAGTCATT 
GAAGCTCCAT CTCTACCTCA ACTGCAGACA CCCCTGGCCA ATGAGTTGGA CCTCATGCCC 
TATACACCCC CACAGCTTTC TGTCTTACAG TCTACCCCAA AGTCTGCCAA AGGCAGTGCA 
AAGAAGGAAA GTTCTAAACG AAAAATCAAC ATGAGTGGCT ACATTTTGTT CAGCAGTGAA 
ATGAGAGCTG TGATTAAAGC CCAGCACCCA GACTACTCTT TTGGGGAGCT CAGCAGACTG 
GTGGGGACAG AATGGAGAAA CCTTGAAACA GCCAAGAAAG CAGAATATGA AGAGCGGGCA 
GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG CGAGCAGCAC AGCAACAGCA GCCGAGTGCT 
TCTCCCCGAG CAGGCACCCC TGTGGGGGCT CTCATGGGGG TGGTGCCACC ACCAACACCA 
ATGGGGATGC TCAATCAGCA GTTGACACCT GTTGCAGGCA TGATGGGTGG CTATCCGCCA 
GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT GGCCTTGTTA GCATGGGCAG CATGCAGCCA 
CTTCACCCTG GGGGGCCTCC ACCTCACCAT CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC 
ATCCCACCAC CGGGTGTGAT GAATCAAGGA GTAGCCCCCA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CGTATGGACA ACAGGTAGGA GTTTTGGGAC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CTGGTCCTCA TCCAGCTGGC CCCCCTGTCA TACAGCAGCC AACAACGCCC 
ATGTTTGTGG CTCCCCCACC AAAGACCCAA AGGCTTCTCC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC TGAATCCAAC AGCATTAGCA AGTGGGACCA AACTTTGGCA 
GCTCGAAGAC GGGATGTCCA TTTGTCCAAA GAACAGGAGA GCCGCCTACC TTCTCACTGG 
CTCAAAAGTA AAGGGGCACA CACCACCATG GCAGATGCCC TCTGGCGCCT ACGGGATTTA 
ATGCTTCGAG ACACTCTCAA CATCCGACAG GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316149.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171638.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X26, mRNA.

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CloneID	OMu78827
Clone ID Related Accession (Same CDS sequence)	XM_017316148.1 , XM_017316149.1
Accession Version	XM_017316149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGGAATA CTCATGACAG TGAGATGTTG GGTCTCAGGA GGCTATCCAG TAAAAAGAAC 
ATAAGAAAAC AGCGAATGAA AATTTTATTC AATGTTGTTC TTGAAGCTCG AGAGCCAGGT 
TCAGGCAGAA GACTTTGCGA TCTATTTATG GTTAAGCCAT CCAAGAAGGA CTATCCTGAT 
TATTATAAAA TCATCTTAGA GCCAATGGAC CTGAAAATAA TTGAGCATAA CATCCGAAAT 
GACAAATATG CAGGTGAAGA AGGAATGATG GAAGACATGA AACTCATGTT CCGCAATGCC 
AGGCACTACA ATGAGGAGGG CTCCCAGGTA TACAATGATG CCCATATCCT GGAGAAGTTA 
CTCAAAGATA AAAGGAAAGA GCTGGGCCCT CTGCCTGATG ATGATGACAT GGCTTCTCCC 
AAACTTAAAT TGAGTAGGAA GAGTGGTGTT TCTCCTAAGA AATCAAAGTA CATGACTCCA 
ATGCAGCAGA AACTGAATGA AGTGTATGAA GCTGTAAAGA ACTATACTGA TAAGAGGGGT 
CGCCGCCTTA GTGCTATATT TCTAAGACTC CCCTCTAGAT CAGAGCTGCC TGACTACTAC 
CTGACCATTA AAAAGCCCAT GGACATGGAA AAAATTCGAA GTCACATGAT GGCAAACAAG 
TACCAAGACA TAGATTCTAT GGTAGAGGAC TTTGTCATGA TGTTTAATAA TGCCTGTACC 
TACAATGAAC CAGAGTCTTT GATCTACAAA GATGCCCTTG TACTGCATAA AGTCCTCCTT 
GAGACTCGGA GAGACCTGGA GGGAGATGAG GATTCTCATG TCCCTAATGT GACGTTGCTG 
ATTCAAGAGC TCATCCATAA CCTTTTTGTG TCAGTCATGA GTCATCAGGA TGACGAAGGG 
AGGTGTTACA GCGACTCCTT AGCAGAAATT CCTGCTGTGG ATCCCAACTC TCCCAATAAA 
CCTCCCCTTA CATTTGACAT TATCAGGAAA AATGTTGAAA GTAATCGGTA TCGGCGACTT 
GATTTATTTC AGGAGCATAT GTTTGAAGTA TTGGAACGGG CAAGAAGGAT GAACCGGACA 
GATTCCGAAA TATATGAGGA TGCTGTAGAA CTTCAGCAGT TTTTTATTAG AATTCGTGAT 
GAACTCTGCA AAAATGGAGA GATCCTTCTT TCTCCAGCAC TCAGCTATAC CACAAAACAC 
TTGCATAACG ATGTGGAAAA AGAAAAAAAG GAAAAATTGC CTAAAGAAAT AGAGGAAGAT 
AAACTAAAAC GCGAAGAAGA AAAAAGAGAA GCTGAAAAAA GTGAAGATTC CTCAGGTACT 
ACAGGCCTCT CAGGCTTACA TCGTACATAC AGCCAGGACT GCAGCTTTAA GAACAGCATG 
TATCATGTCG GAGATTATGT CTATGTTGAA CCTGCGGAGG CCAATCTACA ACCACATATA 
GTGTGTATTG AGAGACTGTG GGAGGATTCA GCTGGTGAAA AATGGTTGTA CGGCTGTTGG 
TTTTATCGGC CAAATGAAAC ATTCCATTTG GCTACACGAA AATTTCTAGA AAAAGAAGTT 
TTTAAGAGTG ACTACTACAA TAAAGTACCT GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC 
ATGTTTGTCA AGGAATACTT TAAATTATGT CCAGAAAACT TTCGCGATGA GGATGTTTTT 
GTCTGTGAAT CGAGGTATTC TGCCAAAACC AAATCTTTTA AGAAAATTAA ACTGTGGACC 
ATGCCCATCA GTTCAGTTAG ATTTGTCCCT CGGGATGTGC CTTTGCCTGT GGTCCGAGTG 
GCCTCTGTGT TTGCAAATGC AGATAAAGGG GATGATGAGA AGAATACAGA CAACTCAGAT 
GACAATAGAG CTGAAGACAA TTTTAACTTG GAAAAGGAAA AAGAAGATGT TCCTGTGGAG 
ATGTCCAATG GTGAGCCAGG TTGCCACTAC TTTGAGCAGC TTCGGTACAA TGACATGTGG 
CTGAAGGTTG GTGATTGTGT CTTCATCAAA TCCCACGGCT TGGTGCGCCC TCGTGTGGGC 
AGAATTGAGA AAGTATGGGT CCGAGATGGA GCTGCATATT TTTATGGCCC TATCTTCATT 
CATCCAGAAG AAACAGAACA TGAGCCCACA AAAATGTTCT ACAAAAAAGA AGTGTTTCTG 
AGTAATCTGG AAGAGACCTG CCCTATGAGT TGTATTCTGG GGAAATGTGC AGTGCTGTCA 
TTCAAGGACT TCCTCTCCTG CAGGCCAACT GAAATACCAG AAAATGACAT TCTGCTTTGT 
GAGAGCCGCT ATAATGAGAG TGACAAGCAG ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT 
TCACTCTCTG CTAAAGTTGT AGATGATGAA ATCTACTACT TCAGAAAACC AATCATTCCT 
CAGAAGGAAC CCTCACCTTT GTTAGAAAAG AAGATACAAT TGCTAGAAGC TAAATTTGCA 
GAGTTAGAAG GAGGAGATGA TGATATTGAG GAGATGGGAG AAGAGGATAG TGAAGTCATT 
GAAGCTCCAT CTCTACCTCA ACTGCAGACA CCCCTGGCCA ATGAGTTGGA CCTCATGCCC 
TATACACCCC CACAGCTTTC TGTCTTACAG TCTACCCCAA AGTCTGCCAA AGGCAGTGCA 
AAGAAGGAAA GTTCTAAACG AAAAATCAAC ATGAGTGGCT ACATTTTGTT CAGCAGTGAA 
ATGAGAGCTG TGATTAAAGC CCAGCACCCA GACTACTCTT TTGGGGAGCT CAGCAGACTG 
GTGGGGACAG AATGGAGAAA CCTTGAAACA GCCAAGAAAG CAGAATATGA AGAGCGGGCA 
GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG CGAGCAGCAC AGCAACAGCA GCCGAGTGCT 
TCTCCCCGAG CAGGCACCCC TGTGGGGGCT CTCATGGGGG TGGTGCCACC ACCAACACCA 
ATGGGGATGC TCAATCAGCA GTTGACACCT GTTGCAGGCA TGATGGGTGG CTATCCGCCA 
GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT GGCCTTGTTA GCATGGGCAG CATGCAGCCA 
CTTCACCCTG GGGGGCCTCC ACCTCACCAT CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC 
ATCCCACCAC CGGGTGTGAT GAATCAAGGA GTAGCCCCCA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CGTATGGACA ACAGGTAGGA GTTTTGGGAC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CTGGTCCTCA TCCAGCTGGC CCCCCTGTCA TACAGCAGCC AACAACGCCC 
ATGTTTGTGG CTCCCCCACC AAAGACCCAA AGGCTTCTCC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC TGAATCCAAC AGCATTAGCA AGTGGGACCA AACTTTGGCA 
GCTCGAAGAC GGGATGTCCA TTTGTCCAAA GAACAGGAGA GCCGCCTACC TTCTCACTGG 
CTCAAAAGTA AAGGGGCACA CACCACCATG GCAGATGCCC TCTGGCGCCT ACGGGATTTA 
ATGCTTCGAG ACACTCTCAA CATCCGACAG GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171638.1
CDS	429..5522
Translation	MRRLAFRGAGCALVKLKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316149.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316149.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGGAATA CTCATGACAG TGAGATGTTG GGTCTCAGGA GGCTATCCAG TAAAAAGAAC 
ATAAGAAAAC AGCGAATGAA AATTTTATTC AATGTTGTTC TTGAAGCTCG AGAGCCAGGT 
TCAGGCAGAA GACTTTGCGA TCTATTTATG GTTAAGCCAT CCAAGAAGGA CTATCCTGAT 
TATTATAAAA TCATCTTAGA GCCAATGGAC CTGAAAATAA TTGAGCATAA CATCCGAAAT 
GACAAATATG CAGGTGAAGA AGGAATGATG GAAGACATGA AACTCATGTT CCGCAATGCC 
AGGCACTACA ATGAGGAGGG CTCCCAGGTA TACAATGATG CCCATATCCT GGAGAAGTTA 
CTCAAAGATA AAAGGAAAGA GCTGGGCCCT CTGCCTGATG ATGATGACAT GGCTTCTCCC 
AAACTTAAAT TGAGTAGGAA GAGTGGTGTT TCTCCTAAGA AATCAAAGTA CATGACTCCA 
ATGCAGCAGA AACTGAATGA AGTGTATGAA GCTGTAAAGA ACTATACTGA TAAGAGGGGT 
CGCCGCCTTA GTGCTATATT TCTAAGACTC CCCTCTAGAT CAGAGCTGCC TGACTACTAC 
CTGACCATTA AAAAGCCCAT GGACATGGAA AAAATTCGAA GTCACATGAT GGCAAACAAG 
TACCAAGACA TAGATTCTAT GGTAGAGGAC TTTGTCATGA TGTTTAATAA TGCCTGTACC 
TACAATGAAC CAGAGTCTTT GATCTACAAA GATGCCCTTG TACTGCATAA AGTCCTCCTT 
GAGACTCGGA GAGACCTGGA GGGAGATGAG GATTCTCATG TCCCTAATGT GACGTTGCTG 
ATTCAAGAGC TCATCCATAA CCTTTTTGTG TCAGTCATGA GTCATCAGGA TGACGAAGGG 
AGGTGTTACA GCGACTCCTT AGCAGAAATT CCTGCTGTGG ATCCCAACTC TCCCAATAAA 
CCTCCCCTTA CATTTGACAT TATCAGGAAA AATGTTGAAA GTAATCGGTA TCGGCGACTT 
GATTTATTTC AGGAGCATAT GTTTGAAGTA TTGGAACGGG CAAGAAGGAT GAACCGGACA 
GATTCCGAAA TATATGAGGA TGCTGTAGAA CTTCAGCAGT TTTTTATTAG AATTCGTGAT 
GAACTCTGCA AAAATGGAGA GATCCTTCTT TCTCCAGCAC TCAGCTATAC CACAAAACAC 
TTGCATAACG ATGTGGAAAA AGAAAAAAAG GAAAAATTGC CTAAAGAAAT AGAGGAAGAT 
AAACTAAAAC GCGAAGAAGA AAAAAGAGAA GCTGAAAAAA GTGAAGATTC CTCAGGTACT 
ACAGGCCTCT CAGGCTTACA TCGTACATAC AGCCAGGACT GCAGCTTTAA GAACAGCATG 
TATCATGTCG GAGATTATGT CTATGTTGAA CCTGCGGAGG CCAATCTACA ACCACATATA 
GTGTGTATTG AGAGACTGTG GGAGGATTCA GCTGGTGAAA AATGGTTGTA CGGCTGTTGG 
TTTTATCGGC CAAATGAAAC ATTCCATTTG GCTACACGAA AATTTCTAGA AAAAGAAGTT 
TTTAAGAGTG ACTACTACAA TAAAGTACCT GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC 
ATGTTTGTCA AGGAATACTT TAAATTATGT CCAGAAAACT TTCGCGATGA GGATGTTTTT 
GTCTGTGAAT CGAGGTATTC TGCCAAAACC AAATCTTTTA AGAAAATTAA ACTGTGGACC 
ATGCCCATCA GTTCAGTTAG ATTTGTCCCT CGGGATGTGC CTTTGCCTGT GGTCCGAGTG 
GCCTCTGTGT TTGCAAATGC AGATAAAGGG GATGATGAGA AGAATACAGA CAACTCAGAT 
GACAATAGAG CTGAAGACAA TTTTAACTTG GAAAAGGAAA AAGAAGATGT TCCTGTGGAG 
ATGTCCAATG GTGAGCCAGG TTGCCACTAC TTTGAGCAGC TTCGGTACAA TGACATGTGG 
CTGAAGGTTG GTGATTGTGT CTTCATCAAA TCCCACGGCT TGGTGCGCCC TCGTGTGGGC 
AGAATTGAGA AAGTATGGGT CCGAGATGGA GCTGCATATT TTTATGGCCC TATCTTCATT 
CATCCAGAAG AAACAGAACA TGAGCCCACA AAAATGTTCT ACAAAAAAGA AGTGTTTCTG 
AGTAATCTGG AAGAGACCTG CCCTATGAGT TGTATTCTGG GGAAATGTGC AGTGCTGTCA 
TTCAAGGACT TCCTCTCCTG CAGGCCAACT GAAATACCAG AAAATGACAT TCTGCTTTGT 
GAGAGCCGCT ATAATGAGAG TGACAAGCAG ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT 
TCACTCTCTG CTAAAGTTGT AGATGATGAA ATCTACTACT TCAGAAAACC AATCATTCCT 
CAGAAGGAAC CCTCACCTTT GTTAGAAAAG AAGATACAAT TGCTAGAAGC TAAATTTGCA 
GAGTTAGAAG GAGGAGATGA TGATATTGAG GAGATGGGAG AAGAGGATAG TGAAGTCATT 
GAAGCTCCAT CTCTACCTCA ACTGCAGACA CCCCTGGCCA ATGAGTTGGA CCTCATGCCC 
TATACACCCC CACAGCTTTC TGTCTTACAG TCTACCCCAA AGTCTGCCAA AGGCAGTGCA 
AAGAAGGAAA GTTCTAAACG AAAAATCAAC ATGAGTGGCT ACATTTTGTT CAGCAGTGAA 
ATGAGAGCTG TGATTAAAGC CCAGCACCCA GACTACTCTT TTGGGGAGCT CAGCAGACTG 
GTGGGGACAG AATGGAGAAA CCTTGAAACA GCCAAGAAAG CAGAATATGA AGAGCGGGCA 
GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG CGAGCAGCAC AGCAACAGCA GCCGAGTGCT 
TCTCCCCGAG CAGGCACCCC TGTGGGGGCT CTCATGGGGG TGGTGCCACC ACCAACACCA 
ATGGGGATGC TCAATCAGCA GTTGACACCT GTTGCAGGCA TGATGGGTGG CTATCCGCCA 
GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT GGCCTTGTTA GCATGGGCAG CATGCAGCCA 
CTTCACCCTG GGGGGCCTCC ACCTCACCAT CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC 
ATCCCACCAC CGGGTGTGAT GAATCAAGGA GTAGCCCCCA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CGTATGGACA ACAGGTAGGA GTTTTGGGAC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CTGGTCCTCA TCCAGCTGGC CCCCCTGTCA TACAGCAGCC AACAACGCCC 
ATGTTTGTGG CTCCCCCACC AAAGACCCAA AGGCTTCTCC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC TGAATCCAAC AGCATTAGCA AGTGGGACCA AACTTTGGCA 
GCTCGAAGAC GGGATGTCCA TTTGTCCAAA GAACAGGAGA GCCGCCTACC TTCTCACTGG 
CTCAAAAGTA AAGGGGCACA CACCACCATG GCAGATGCCC TCTGGCGCCT ACGGGATTTA 
ATGCTTCGAG ACACTCTCAA CATCCGACAG GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316151.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171640.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X28, mRNA.

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CloneID	OMu78828
Clone ID Related Accession (Same CDS sequence)	XM_017316151.1 , XM_017316150.1
Accession Version	XM_017316151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGTAATAAA 
AGAGCAGTCC AAGGGGGTCG CTTGTCAGCA ATTACCATGG CACTTCAGTA TGGATCAGAG 
AGTGAAGAGG ACGCTGCTTT AGCTGCTGCA CGCTATGAAG AAGGGGAATC TGAAGCAGAG 
AGCATCACTT CCTTCATGGA CGTTTCCAAC CCCTTTCATC AGCTTTACGA CACAGTTAGG 
AGCTGTAGGA ATCACCAAGG GCAGCTCATA GCTGAACCTT TCTTCCATTT GCCTTCAAAG 
AAAAAATACC CAGATTATTA TCAGCAAATT AAAATGCCCA TATCACTTCA ACAGATCAGA 
ACAAAGCTAA AGAACCAAGA ATATGAAACT TTAGATCATT TGGAGTGTGA TCTGAATTTA 
ATGTTTGAAA ATGCCAAACG TTATAACGTT CCCAATTCAG CCATCTATAA GCGAGTTCTA 
AAACTGCAGC AAGTCATGCA GGCAAAGAAG AAGGAGCTTG CGAGGAGAGA TGACATTGAG 
GACGGAGACA GCATGATCTC CTCAGCCACT TCTGACACTG GTAGTGCCAA AAGGAAAAGG 
AATACTCATG ACAGTGAGAT GTTGGGTCTC AGGAGGCTAT CCAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGC TTTCTGTCTT ACAGTCTACC CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG 
GAAAGTTCTA AACGAAAAAT CAACATGAGT GGCTACATTT TGTTCAGCAG TGAAATGAGA 
GCTGTGATTA AAGCCCAGCA CCCAGACTAC TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG 
ACAGAATGGA GAAACCTTGA AACAGCCAAG AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA 
GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA GCACAGCAAC AGCAGCCGAG TGCTTCTCCC 
CGAGCAGGCA CCCCTGTGGG GGCTCTCATG GGGGTGGTGC CACCACCAAC ACCAATGGGG 
ATGCTCAATC AGCAGTTGAC ACCTGTTGCA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171640.1
CDS	429..5516
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316151.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316151.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGTAATAAA 
AGAGCAGTCC AAGGGGGTCG CTTGTCAGCA ATTACCATGG CACTTCAGTA TGGATCAGAG 
AGTGAAGAGG ACGCTGCTTT AGCTGCTGCA CGCTATGAAG AAGGGGAATC TGAAGCAGAG 
AGCATCACTT CCTTCATGGA CGTTTCCAAC CCCTTTCATC AGCTTTACGA CACAGTTAGG 
AGCTGTAGGA ATCACCAAGG GCAGCTCATA GCTGAACCTT TCTTCCATTT GCCTTCAAAG 
AAAAAATACC CAGATTATTA TCAGCAAATT AAAATGCCCA TATCACTTCA ACAGATCAGA 
ACAAAGCTAA AGAACCAAGA ATATGAAACT TTAGATCATT TGGAGTGTGA TCTGAATTTA 
ATGTTTGAAA ATGCCAAACG TTATAACGTT CCCAATTCAG CCATCTATAA GCGAGTTCTA 
AAACTGCAGC AAGTCATGCA GGCAAAGAAG AAGGAGCTTG CGAGGAGAGA TGACATTGAG 
GACGGAGACA GCATGATCTC CTCAGCCACT TCTGACACTG GTAGTGCCAA AAGGAAAAGG 
AATACTCATG ACAGTGAGAT GTTGGGTCTC AGGAGGCTAT CCAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGC TTTCTGTCTT ACAGTCTACC CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG 
GAAAGTTCTA AACGAAAAAT CAACATGAGT GGCTACATTT TGTTCAGCAG TGAAATGAGA 
GCTGTGATTA AAGCCCAGCA CCCAGACTAC TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG 
ACAGAATGGA GAAACCTTGA AACAGCCAAG AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA 
GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA GCACAGCAAC AGCAGCCGAG TGCTTCTCCC 
CGAGCAGGCA CCCCTGTGGG GGCTCTCATG GGGGTGGTGC CACCACCAAC ACCAATGGGG 
ATGCTCAATC AGCAGTTGAC ACCTGTTGCA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316150.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171639.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X27, mRNA.

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CloneID	OMu78828
Clone ID Related Accession (Same CDS sequence)	XM_017316151.1 , XM_017316150.1
Accession Version	XM_017316150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGTAATAAA 
AGAGCAGTCC AAGGGGGTCG CTTGTCAGCA ATTACCATGG CACTTCAGTA TGGATCAGAG 
AGTGAAGAGG ACGCTGCTTT AGCTGCTGCA CGCTATGAAG AAGGGGAATC TGAAGCAGAG 
AGCATCACTT CCTTCATGGA CGTTTCCAAC CCCTTTCATC AGCTTTACGA CACAGTTAGG 
AGCTGTAGGA ATCACCAAGG GCAGCTCATA GCTGAACCTT TCTTCCATTT GCCTTCAAAG 
AAAAAATACC CAGATTATTA TCAGCAAATT AAAATGCCCA TATCACTTCA ACAGATCAGA 
ACAAAGCTAA AGAACCAAGA ATATGAAACT TTAGATCATT TGGAGTGTGA TCTGAATTTA 
ATGTTTGAAA ATGCCAAACG TTATAACGTT CCCAATTCAG CCATCTATAA GCGAGTTCTA 
AAACTGCAGC AAGTCATGCA GGCAAAGAAG AAGGAGCTTG CGAGGAGAGA TGACATTGAG 
GACGGAGACA GCATGATCTC CTCAGCCACT TCTGACACTG GTAGTGCCAA AAGGAAAAGG 
AATACTCATG ACAGTGAGAT GTTGGGTCTC AGGAGGCTAT CCAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGC TTTCTGTCTT ACAGTCTACC CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG 
GAAAGTTCTA AACGAAAAAT CAACATGAGT GGCTACATTT TGTTCAGCAG TGAAATGAGA 
GCTGTGATTA AAGCCCAGCA CCCAGACTAC TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG 
ACAGAATGGA GAAACCTTGA AACAGCCAAG AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA 
GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA GCACAGCAAC AGCAGCCGAG TGCTTCTCCC 
CGAGCAGGCA CCCCTGTGGG GGCTCTCATG GGGGTGGTGC CACCACCAAC ACCAATGGGG 
ATGCTCAATC AGCAGTTGAC ACCTGTTGCA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171639.1
CDS	359..5446
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316150.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316150.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGTAATAAA 
AGAGCAGTCC AAGGGGGTCG CTTGTCAGCA ATTACCATGG CACTTCAGTA TGGATCAGAG 
AGTGAAGAGG ACGCTGCTTT AGCTGCTGCA CGCTATGAAG AAGGGGAATC TGAAGCAGAG 
AGCATCACTT CCTTCATGGA CGTTTCCAAC CCCTTTCATC AGCTTTACGA CACAGTTAGG 
AGCTGTAGGA ATCACCAAGG GCAGCTCATA GCTGAACCTT TCTTCCATTT GCCTTCAAAG 
AAAAAATACC CAGATTATTA TCAGCAAATT AAAATGCCCA TATCACTTCA ACAGATCAGA 
ACAAAGCTAA AGAACCAAGA ATATGAAACT TTAGATCATT TGGAGTGTGA TCTGAATTTA 
ATGTTTGAAA ATGCCAAACG TTATAACGTT CCCAATTCAG CCATCTATAA GCGAGTTCTA 
AAACTGCAGC AAGTCATGCA GGCAAAGAAG AAGGAGCTTG CGAGGAGAGA TGACATTGAG 
GACGGAGACA GCATGATCTC CTCAGCCACT TCTGACACTG GTAGTGCCAA AAGGAAAAGG 
AATACTCATG ACAGTGAGAT GTTGGGTCTC AGGAGGCTAT CCAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGC TTTCTGTCTT ACAGTCTACC CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG 
GAAAGTTCTA AACGAAAAAT CAACATGAGT GGCTACATTT TGTTCAGCAG TGAAATGAGA 
GCTGTGATTA AAGCCCAGCA CCCAGACTAC TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG 
ACAGAATGGA GAAACCTTGA AACAGCCAAG AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA 
GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA GCACAGCAAC AGCAGCCGAG TGCTTCTCCC 
CGAGCAGGCA CCCCTGTGGG GGCTCTCATG GGGGTGGTGC CACCACCAAC ACCAATGGGG 
ATGCTCAATC AGCAGTTGAC ACCTGTTGCA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316152.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171641.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X30, mRNA.

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CloneID	OMu78829
Clone ID Related Accession (Same CDS sequence)	XM_017316152.1
Accession Version	XM_017316152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGTTGGATTC CATGGGTTCC 
AAGAGAAGAA GAGCCACCTC TCCTTCCAGC AGTGTCAGTG GAGACTTTGA TGACGGGCAC 
CATTCTGTGC CTACACCAGG CCCAAGCAGG AAAAGGAGAA GACTGTCCAA TCTTCCAACT 
GTAGATCCTA TTGCTGTGTG CCATGAACTC TATAACACCA TCCGAGACTA TAAGGATGAA 
CAGGGCAGAC TCCTCTGTGA GCTGTTCATT AGGGCTCCAA AGCGGAGAAA TCAACCAGAC 
TATTATGAAG TGGTTTCTCA GCCCATTGAC TTGATGAAAA TCCAACAGAA ACTTAAAATG 
GAAGAGTATG ATGATGTTAA TCTACTGACT GCTGACTTCC AGCTGCTTTT TAACAATGCA 
AAGGCCTACT ATAAGCCAGA TTCCCCTGAG TATAAAGCTG CTTGTAAACT CTGGGATTTG 
TACCTTCGAA CAAGAAATGA GTTTGTTCAG AAAGGAGAAG CAGACGATGA AGATGATGAC 
GAAGATGGGC AAGACAATCA AGGCACACTG GCTGACGGCT CTTCTCCAGG TTATCTGAAG 
GAGATCCTGG AGCAGCTTCT TGAAGCCATA GTTGTAGCCA CAAATCCATC AGGACGGCTC 
ATCAGTGAAC TTTTTCAGAA ACTGCCTTCC AAAGTGCAAT ATCCAGACTA TTATGCAATA 
ATTAAGGAAC CTATAGATCT CAAGACCATT GCTCAGAGGA TACAGAATGG AAGCTACAAA 
AGTATACACG CAATGGCCAA AGATATAGAT CTTCTAGCAA AAAATGCCAA AACATACAAT 
GAGCCTGGGT CTCAAGTATT CAAGGATGCC AATTCGATTA AAAAAATATT TTATATGAAA 
AAGGCAGAAA TTGAACATCA TGAAATGACT AAATCAAGTC TTCGAATAAG TAATAAAAGA 
GCAGTCCAAG GGGGTCGCTT GTCAGCAATT ACCATGGCAC TTCAGTATGG ATCAGAGAGT 
GAAGAGGACG CTGCTTTAGC TGCTGCACGC TATGAAGAAG GGGAATCTGA AGCAGAGAGC 
ATCACTTCCT TCATGGACGT TTCCAACCCC TTTCATCAGC TTTACGACAC AGTTAGGAGC 
TGTAGGAATC ACCAAGGGCA GCTCATAGCT GAACCTTTCT TCCATTTGCC TTCAAAGAAA 
AAATACCCAG ATTATTATCA GCAAATTAAA ATGCCCATAT CACTTCAACA GATCAGAACA 
AAGCTAAAGA ACCAAGAATA TGAAACTTTA GATCATTTGG AGTGTGATCT GAATTTAATG 
TTTGAAAATG CCAAACGTTA TAACGTTCCC AATTCAGCCA TCTATAAGCG AGTTCTAAAA 
CTGCAGCAAG TCATGCAGGC AAAGAAGAAG GAGCTTGCGA GGAGAGATGA CATTGAGGAC 
GGAGACAGCA TGATCTCCTC AGCCACTTCT GACACTGGTA GTGCCAAAAG GAAAAGGAAT 
ACTCATGACA GTGAGATGTT GGGTCTCAGG AGGCTATCCA GTAAAAAGAA CATAAGAAAA 
CAGCGAATGA AAATTTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGCG ATCTATTTAT GGTTAAGCCA TCCAAGAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTAG AGCCAATGGA CCTGAAAATA ATTGAGCATA ACATCCGAAA TGACAAATAT 
GCAGGTGAAG AAGGAATGAT GGAAGACATG AAACTCATGT TCCGCAATGC CAGGCACTAC 
AATGAGGAGG GCTCCCAGGT ATACAATGAT GCCCATATCC TGGAGAAGTT ACTCAAAGAT 
AAAAGGAAAG AGCTGGGCCC TCTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTTAAA 
TTGAGTAGGA AGAGTGGTGT TTCTCCTAAG AAATCAAAGT ACATGACTCC AATGCAGCAG 
AAACTGAATG AAGTGTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTT 
AGTGCTATAT TTCTAAGACT CCCCTCTAGA TCAGAGCTGC CTGACTACTA CCTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCAAACAA GTACCAAGAC 
ATAGATTCTA TGGTAGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC CTACAATGAA 
CCAGAGTCTT TGATCTACAA AGATGCCCTT GTACTGCATA AAGTCCTCCT TGAGACTCGG 
AGAGACCTGG AGGGAGATGA GGATTCTCAT GTCCCTAATG TGACGTTGCT GATTCAAGAG 
CTCATCCATA ACCTTTTTGT GTCAGTCATG AGTCATCAGG ATGACGAAGG GAGGTGTTAC 
AGCGACTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAACT CTCCCAATAA ACCTCCCCTT 
ACATTTGACA TTATCAGGAA AAATGTTGAA AGTAATCGGT ATCGGCGACT TGATTTATTT 
CAGGAGCATA TGTTTGAAGT ATTGGAACGG GCAAGAAGGA TGAACCGGAC AGATTCCGAA 
ATATATGAGG ATGCTGTAGA ACTTCAGCAG TTTTTTATTA GAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCTCCAGCA CTCAGCTATA CCACAAAACA CTTGCATAAC 
GATGTGGAAA AAGAAAAAAA GGAAAAATTG CCTAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGCGAAGAAG AAAAAAGAGA AGCTGAAAAA AGTGAAGATT CCTCAGGTAC TACAGGCCTC 
TCAGGCTTAC ATCGTACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTATCATGTC 
GGAGATTATG TCTATGTTGA ACCTGCGGAG GCCAATCTAC AACCACATAT AGTGTGTATT 
GAGAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ACGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTACTACA ATAAAGTACC TGTTAGTAAA ATTCTAGGCA AATGTGTAGT CATGTTTGTC 
AAGGAATACT TTAAATTATG TCCAGAAAAC TTTCGCGATG AGGATGTTTT TGTCTGTGAA 
TCGAGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGTTCAGTTA GATTTGTCCC TCGGGATGTG CCTTTGCCTG TGGTCCGAGT GGCCTCTGTG 
TTTGCAAATG CAGATAAAGG GGATGATGAG AAGAATACAG ACAACTCAGA TGACAATAGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TTCCTGTGGA GATGTCCAAT 
GGTGAGCCAG GTTGCCACTA CTTTGAGCAG CTTCGGTACA ATGACATGTG GCTGAAGGTT 
GGTGATTGTG TCTTCATCAA ATCCCACGGC TTGGTGCGCC CTCGTGTGGG CAGAATTGAG 
AAAGTATGGG TCCGAGATGG AGCTGCATAT TTTTATGGCC CTATCTTCAT TCATCCAGAA 
GAAACAGAAC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTGTTTCT GAGTAATCTG 
GAAGAGACCT GCCCTATGAG TTGTATTCTG GGGAAATGTG CAGTGCTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TATAATGAGA GTGACAAGCA GATGAAGAAG TTCAAGGGTT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTTG TAGATGATGA AATCTACTAC TTCAGAAAAC CAATCATTCC TCAGAAGGAA 
CCCTCACCTT TGTTAGAAAA GAAGATACAA TTGCTAGAAG CTAAATTTGC AGAGTTAGAA 
GGAGGAGATG ATGATATTGA GGAGATGGGA GAAGAGGATA GTGAAGTCAT TGAAGCTCCA 
TCTCTACCTC AACTGCAGAC ACCCCTGGCC AATGAGTTGG ACCTCATGCC CTATACACCC 
CCACAGCTTT CTGTCTTACA GTCTACCCCA AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA 
AGTTCTAAAC GAAAAATCAA CATGAGTGGC TACATTTTGT TCAGCAGTGA AATGAGAGCT 
GTGATTAAAG CCCAGCACCC AGACTACTCT TTTGGGGAGC TCAGCAGACT GGTGGGGACA 
GAATGGAGAA ACCTTGAAAC AGCCAAGAAA GCAGAATATG AAGAGCGGGC AGCTAAAGTT 
GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA CAGCAACAGC AGCCGAGTGC TTCTCCCCGA 
GCAGGCACCC CTGTGGGGGC TCTCATGGGG GTGGTGCCAC CACCAACACC AATGGGGATG 
CTCAATCAGC AGTTGACACC TGTTGCAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCTCACCA TCTTCCGCCA GGTGTGCCTG GCCTCCCAGG CATCCCACCA 
CCGGGTGTGA TGAATCAAGG AGTAGCCCCC ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AACAGGTAGG AGTTTTGGGA CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGTCCTC ATCCAGCTGG CCCCCCTGTC ATACAGCAGC CAACAACGCC CATGTTTGTG 
GCTCCCCCAC CAAAGACCCA AAGGCTTCTC CACTCAGAGG CCTACCTGAA ATACATTGAA 
GGACTCAGTG CTGAATCCAA CAGCATTAGC AAGTGGGACC AAACTTTGGC AGCTCGAAGA 
CGGGATGTCC ATTTGTCCAA AGAACAGGAG AGCCGCCTAC CTTCTCACTG GCTCAAAAGT 
AAAGGGGCAC ACACCACCAT GGCAGATGCC CTCTGGCGCC TACGGGATTT AATGCTTCGA 
GACACTCTCA ACATCCGACA GGCATACAAC CTAGAAAATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171641.1
CDS	342..5426
Translation	MRRLAFRGAGCALKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316152.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316152.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGTTGGATTC CATGGGTTCC 
AAGAGAAGAA GAGCCACCTC TCCTTCCAGC AGTGTCAGTG GAGACTTTGA TGACGGGCAC 
CATTCTGTGC CTACACCAGG CCCAAGCAGG AAAAGGAGAA GACTGTCCAA TCTTCCAACT 
GTAGATCCTA TTGCTGTGTG CCATGAACTC TATAACACCA TCCGAGACTA TAAGGATGAA 
CAGGGCAGAC TCCTCTGTGA GCTGTTCATT AGGGCTCCAA AGCGGAGAAA TCAACCAGAC 
TATTATGAAG TGGTTTCTCA GCCCATTGAC TTGATGAAAA TCCAACAGAA ACTTAAAATG 
GAAGAGTATG ATGATGTTAA TCTACTGACT GCTGACTTCC AGCTGCTTTT TAACAATGCA 
AAGGCCTACT ATAAGCCAGA TTCCCCTGAG TATAAAGCTG CTTGTAAACT CTGGGATTTG 
TACCTTCGAA CAAGAAATGA GTTTGTTCAG AAAGGAGAAG CAGACGATGA AGATGATGAC 
GAAGATGGGC AAGACAATCA AGGCACACTG GCTGACGGCT CTTCTCCAGG TTATCTGAAG 
GAGATCCTGG AGCAGCTTCT TGAAGCCATA GTTGTAGCCA CAAATCCATC AGGACGGCTC 
ATCAGTGAAC TTTTTCAGAA ACTGCCTTCC AAAGTGCAAT ATCCAGACTA TTATGCAATA 
ATTAAGGAAC CTATAGATCT CAAGACCATT GCTCAGAGGA TACAGAATGG AAGCTACAAA 
AGTATACACG CAATGGCCAA AGATATAGAT CTTCTAGCAA AAAATGCCAA AACATACAAT 
GAGCCTGGGT CTCAAGTATT CAAGGATGCC AATTCGATTA AAAAAATATT TTATATGAAA 
AAGGCAGAAA TTGAACATCA TGAAATGACT AAATCAAGTC TTCGAATAAG TAATAAAAGA 
GCAGTCCAAG GGGGTCGCTT GTCAGCAATT ACCATGGCAC TTCAGTATGG ATCAGAGAGT 
GAAGAGGACG CTGCTTTAGC TGCTGCACGC TATGAAGAAG GGGAATCTGA AGCAGAGAGC 
ATCACTTCCT TCATGGACGT TTCCAACCCC TTTCATCAGC TTTACGACAC AGTTAGGAGC 
TGTAGGAATC ACCAAGGGCA GCTCATAGCT GAACCTTTCT TCCATTTGCC TTCAAAGAAA 
AAATACCCAG ATTATTATCA GCAAATTAAA ATGCCCATAT CACTTCAACA GATCAGAACA 
AAGCTAAAGA ACCAAGAATA TGAAACTTTA GATCATTTGG AGTGTGATCT GAATTTAATG 
TTTGAAAATG CCAAACGTTA TAACGTTCCC AATTCAGCCA TCTATAAGCG AGTTCTAAAA 
CTGCAGCAAG TCATGCAGGC AAAGAAGAAG GAGCTTGCGA GGAGAGATGA CATTGAGGAC 
GGAGACAGCA TGATCTCCTC AGCCACTTCT GACACTGGTA GTGCCAAAAG GAAAAGGAAT 
ACTCATGACA GTGAGATGTT GGGTCTCAGG AGGCTATCCA GTAAAAAGAA CATAAGAAAA 
CAGCGAATGA AAATTTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGCG ATCTATTTAT GGTTAAGCCA TCCAAGAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTAG AGCCAATGGA CCTGAAAATA ATTGAGCATA ACATCCGAAA TGACAAATAT 
GCAGGTGAAG AAGGAATGAT GGAAGACATG AAACTCATGT TCCGCAATGC CAGGCACTAC 
AATGAGGAGG GCTCCCAGGT ATACAATGAT GCCCATATCC TGGAGAAGTT ACTCAAAGAT 
AAAAGGAAAG AGCTGGGCCC TCTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTTAAA 
TTGAGTAGGA AGAGTGGTGT TTCTCCTAAG AAATCAAAGT ACATGACTCC AATGCAGCAG 
AAACTGAATG AAGTGTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTT 
AGTGCTATAT TTCTAAGACT CCCCTCTAGA TCAGAGCTGC CTGACTACTA CCTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCAAACAA GTACCAAGAC 
ATAGATTCTA TGGTAGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC CTACAATGAA 
CCAGAGTCTT TGATCTACAA AGATGCCCTT GTACTGCATA AAGTCCTCCT TGAGACTCGG 
AGAGACCTGG AGGGAGATGA GGATTCTCAT GTCCCTAATG TGACGTTGCT GATTCAAGAG 
CTCATCCATA ACCTTTTTGT GTCAGTCATG AGTCATCAGG ATGACGAAGG GAGGTGTTAC 
AGCGACTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAACT CTCCCAATAA ACCTCCCCTT 
ACATTTGACA TTATCAGGAA AAATGTTGAA AGTAATCGGT ATCGGCGACT TGATTTATTT 
CAGGAGCATA TGTTTGAAGT ATTGGAACGG GCAAGAAGGA TGAACCGGAC AGATTCCGAA 
ATATATGAGG ATGCTGTAGA ACTTCAGCAG TTTTTTATTA GAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCTCCAGCA CTCAGCTATA CCACAAAACA CTTGCATAAC 
GATGTGGAAA AAGAAAAAAA GGAAAAATTG CCTAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGCGAAGAAG AAAAAAGAGA AGCTGAAAAA AGTGAAGATT CCTCAGGTAC TACAGGCCTC 
TCAGGCTTAC ATCGTACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTATCATGTC 
GGAGATTATG TCTATGTTGA ACCTGCGGAG GCCAATCTAC AACCACATAT AGTGTGTATT 
GAGAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ACGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTACTACA ATAAAGTACC TGTTAGTAAA ATTCTAGGCA AATGTGTAGT CATGTTTGTC 
AAGGAATACT TTAAATTATG TCCAGAAAAC TTTCGCGATG AGGATGTTTT TGTCTGTGAA 
TCGAGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGTTCAGTTA GATTTGTCCC TCGGGATGTG CCTTTGCCTG TGGTCCGAGT GGCCTCTGTG 
TTTGCAAATG CAGATAAAGG GGATGATGAG AAGAATACAG ACAACTCAGA TGACAATAGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TTCCTGTGGA GATGTCCAAT 
GGTGAGCCAG GTTGCCACTA CTTTGAGCAG CTTCGGTACA ATGACATGTG GCTGAAGGTT 
GGTGATTGTG TCTTCATCAA ATCCCACGGC TTGGTGCGCC CTCGTGTGGG CAGAATTGAG 
AAAGTATGGG TCCGAGATGG AGCTGCATAT TTTTATGGCC CTATCTTCAT TCATCCAGAA 
GAAACAGAAC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTGTTTCT GAGTAATCTG 
GAAGAGACCT GCCCTATGAG TTGTATTCTG GGGAAATGTG CAGTGCTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TATAATGAGA GTGACAAGCA GATGAAGAAG TTCAAGGGTT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTTG TAGATGATGA AATCTACTAC TTCAGAAAAC CAATCATTCC TCAGAAGGAA 
CCCTCACCTT TGTTAGAAAA GAAGATACAA TTGCTAGAAG CTAAATTTGC AGAGTTAGAA 
GGAGGAGATG ATGATATTGA GGAGATGGGA GAAGAGGATA GTGAAGTCAT TGAAGCTCCA 
TCTCTACCTC AACTGCAGAC ACCCCTGGCC AATGAGTTGG ACCTCATGCC CTATACACCC 
CCACAGCTTT CTGTCTTACA GTCTACCCCA AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA 
AGTTCTAAAC GAAAAATCAA CATGAGTGGC TACATTTTGT TCAGCAGTGA AATGAGAGCT 
GTGATTAAAG CCCAGCACCC AGACTACTCT TTTGGGGAGC TCAGCAGACT GGTGGGGACA 
GAATGGAGAA ACCTTGAAAC AGCCAAGAAA GCAGAATATG AAGAGCGGGC AGCTAAAGTT 
GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA CAGCAACAGC AGCCGAGTGC TTCTCCCCGA 
GCAGGCACCC CTGTGGGGGC TCTCATGGGG GTGGTGCCAC CACCAACACC AATGGGGATG 
CTCAATCAGC AGTTGACACC TGTTGCAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCTCACCA TCTTCCGCCA GGTGTGCCTG GCCTCCCAGG CATCCCACCA 
CCGGGTGTGA TGAATCAAGG AGTAGCCCCC ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AACAGGTAGG AGTTTTGGGA CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGTCCTC ATCCAGCTGG CCCCCCTGTC ATACAGCAGC CAACAACGCC CATGTTTGTG 
GCTCCCCCAC CAAAGACCCA AAGGCTTCTC CACTCAGAGG CCTACCTGAA ATACATTGAA 
GGACTCAGTG CTGAATCCAA CAGCATTAGC AAGTGGGACC AAACTTTGGC AGCTCGAAGA 
CGGGATGTCC ATTTGTCCAA AGAACAGGAG AGCCGCCTAC CTTCTCACTG GCTCAAAAGT 
AAAGGGGCAC ACACCACCAT GGCAGATGCC CTCTGGCGCC TACGGGATTT AATGCTTCGA 
GACACTCTCA ACATCCGACA GGCATACAAC CTAGAAAATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316153.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171642.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X31, mRNA.

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CloneID	OMu78830
Clone ID Related Accession (Same CDS sequence)	XM_017316153.1
Accession Version	XM_017316153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGTAATAAA 
AGAGCAGTCC AAGGGGGTCG CTTGTCAGCA ATTACCATGG CACTTCAGTA TGGATCAGAG 
AGTGAAGAGG ACGCTGCTTT AGCTGCTGCA CGCTATGAAG AAGGGGAATC TGAAGCAGAG 
AGCATCACTT CCTTCATGGA CGTTTCCAAC CCCTTTCATC AGCTTTACGA CACAGTTAGG 
AGCTGTAGGA ATCACCAAGG GCAGCTCATA GCTGAACCTT TCTTCCATTT GCCTTCAAAG 
AAAAAATACC CAGATTATTA TCAGCAAATT AAAATGCCCA TATCACTTCA ACAGATCAGA 
ACAAAGCTAA AGAACCAAGA ATATGAAACT TTAGATCATT TGGAGTGTGA TCTGAATTTA 
ATGTTTGAAA ATGCCAAACG TTATAACGTT CCCAATTCAG CCATCTATAA GCGAGTTCTA 
AAACTGCAGC AAGTCATGCA GGCAAAGAAG AAGGAGCTTG CGAGGAGAGA TGACATTGAG 
GACGGAGACA GCATGATCTC CTCAGCCACT TCTGACACTG GTAGTGCCAA AAGGAAAAGG 
AATACTCATG ACAGTGAGAT GTTGGGTCTC AGGAGGCTAT CCAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171642.1
CDS	358..5430
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316153.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316153.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGTAATAAA 
AGAGCAGTCC AAGGGGGTCG CTTGTCAGCA ATTACCATGG CACTTCAGTA TGGATCAGAG 
AGTGAAGAGG ACGCTGCTTT AGCTGCTGCA CGCTATGAAG AAGGGGAATC TGAAGCAGAG 
AGCATCACTT CCTTCATGGA CGTTTCCAAC CCCTTTCATC AGCTTTACGA CACAGTTAGG 
AGCTGTAGGA ATCACCAAGG GCAGCTCATA GCTGAACCTT TCTTCCATTT GCCTTCAAAG 
AAAAAATACC CAGATTATTA TCAGCAAATT AAAATGCCCA TATCACTTCA ACAGATCAGA 
ACAAAGCTAA AGAACCAAGA ATATGAAACT TTAGATCATT TGGAGTGTGA TCTGAATTTA 
ATGTTTGAAA ATGCCAAACG TTATAACGTT CCCAATTCAG CCATCTATAA GCGAGTTCTA 
AAACTGCAGC AAGTCATGCA GGCAAAGAAG AAGGAGCTTG CGAGGAGAGA TGACATTGAG 
GACGGAGACA GCATGATCTC CTCAGCCACT TCTGACACTG GTAGTGCCAA AAGGAAAAGG 
AATACTCATG ACAGTGAGAT GTTGGGTCTC AGGAGGCTAT CCAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316154.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171643.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OMu78831
Clone ID Related Accession (Same CDS sequence)	XM_017316154.1
Accession Version	XM_017316154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGTAAAA AGAACATAAG AAAACAGCGA ATGAAAATTT TATTCAATGT TGTTCTTGAA 
GCTCGAGAGC CAGGTTCAGG CAGAAGACTT TGCGATCTAT TTATGGTTAA GCCATCCAAG 
AAGGACTATC CTGATTATTA TAAAATCATC TTAGAGCCAA TGGACCTGAA AATAATTGAG 
CATAACATCC GAAATGACAA ATATGCAGGT GAAGAAGGAA TGATGGAAGA CATGAAACTC 
ATGTTCCGCA ATGCCAGGCA CTACAATGAG GAGGGCTCCC AGGTATACAA TGATGCCCAT 
ATCCTGGAGA AGTTACTCAA AGATAAAAGG AAAGAGCTGG GCCCTCTGCC TGATGATGAT 
GACATGGCTT CTCCCAAACT TAAATTGAGT AGGAAGAGTG GTGTTTCTCC TAAGAAATCA 
AAGTACATGA CTCCAATGCA GCAGAAACTG AATGAAGTGT ATGAAGCTGT AAAGAACTAT 
ACTGATAAGA GGGGTCGCCG CCTTAGTGCT ATATTTCTAA GACTCCCCTC TAGATCAGAG 
CTGCCTGACT ACTACCTGAC CATTAAAAAG CCCATGGACA TGGAAAAAAT TCGAAGTCAC 
ATGATGGCAA ACAAGTACCA AGACATAGAT TCTATGGTAG AGGACTTTGT CATGATGTTT 
AATAATGCCT GTACCTACAA TGAACCAGAG TCTTTGATCT ACAAAGATGC CCTTGTACTG 
CATAAAGTCC TCCTTGAGAC TCGGAGAGAC CTGGAGGGAG ATGAGGATTC TCATGTCCCT 
AATGTGACGT TGCTGATTCA AGAGCTCATC CATAACCTTT TTGTGTCAGT CATGAGTCAT 
CAGGATGACG AAGGGAGGTG TTACAGCGAC TCCTTAGCAG AAATTCCTGC TGTGGATCCC 
AACTCTCCCA ATAAACCTCC CCTTACATTT GACATTATCA GGAAAAATGT TGAAAGTAAT 
CGGTATCGGC GACTTGATTT ATTTCAGGAG CATATGTTTG AAGTATTGGA ACGGGCAAGA 
AGGATGAACC GGACAGATTC CGAAATATAT GAGGATGCTG TAGAACTTCA GCAGTTTTTT 
ATTAGAATTC GTGATGAACT CTGCAAAAAT GGAGAGATCC TTCTTTCTCC AGCACTCAGC 
TATACCACAA AACACTTGCA TAACGATGTG GAAAAAGAAA AAAAGGAAAA ATTGCCTAAA 
GAAATAGAGG AAGATAAACT AAAACGCGAA GAAGAAAAAA GAGAAGCTGA AAAAAGTGAA 
GATTCCTCAG GTACTACAGG CCTCTCAGGC TTACATCGTA CATACAGCCA GGACTGCAGC 
TTTAAGAACA GCATGTATCA TGTCGGAGAT TATGTCTATG TTGAACCTGC GGAGGCCAAT 
CTACAACCAC ATATAGTGTG TATTGAGAGA CTGTGGGAGG ATTCAGCTGG TGAAAAATGG 
TTGTACGGCT GTTGGTTTTA TCGGCCAAAT GAAACATTCC ATTTGGCTAC ACGAAAATTT 
CTAGAAAAAG AAGTTTTTAA GAGTGACTAC TACAATAAAG TACCTGTTAG TAAAATTCTA 
GGCAAATGTG TAGTCATGTT TGTCAAGGAA TACTTTAAAT TATGTCCAGA AAACTTTCGC 
GATGAGGATG TTTTTGTCTG TGAATCGAGG TATTCTGCCA AAACCAAATC TTTTAAGAAA 
ATTAAACTGT GGACCATGCC CATCAGTTCA GTTAGATTTG TCCCTCGGGA TGTGCCTTTG 
CCTGTGGTCC GAGTGGCCTC TGTGTTTGCA AATGCAGATA AAGGGGATGA TGAGAAGAAT 
ACAGACAACT CAGATGACAA TAGAGCTGAA GACAATTTTA ACTTGGAAAA GGAAAAAGAA 
GATGTTCCTG TGGAGATGTC CAATGGTGAG CCAGGTTGCC ACTACTTTGA GCAGCTTCGG 
TACAATGACA TGTGGCTGAA GGTTGGTGAT TGTGTCTTCA TCAAATCCCA CGGCTTGGTG 
CGCCCTCGTG TGGGCAGAAT TGAGAAAGTA TGGGTCCGAG ATGGAGCTGC ATATTTTTAT 
GGCCCTATCT TCATTCATCC AGAAGAAACA GAACATGAGC CCACAAAAAT GTTCTACAAA 
AAAGAAGTGT TTCTGAGTAA TCTGGAAGAG ACCTGCCCTA TGAGTTGTAT TCTGGGGAAA 
TGTGCAGTGC TGTCATTCAA GGACTTCCTC TCCTGCAGGC CAACTGAAAT ACCAGAAAAT 
GACATTCTGC TTTGTGAGAG CCGCTATAAT GAGAGTGACA AGCAGATGAA GAAGTTCAAG 
GGTTTGAAGA GGTTTTCACT CTCTGCTAAA GTTGTAGATG ATGAAATCTA CTACTTCAGA 
AAACCAATCA TTCCTCAGAA GGAACCCTCA CCTTTGTTAG AAAAGAAGAT ACAATTGCTA 
GAAGCTAAAT TTGCAGAGTT AGAAGGAGGA GATGATGATA TTGAGGAGAT GGGAGAAGAG 
GATAGTGAAG TCATTGAAGC TCCATCTCTA CCTCAACTGC AGACACCCCT GGCCAATGAG 
TTGGACCTCA TGCCCTATAC ACCCCCACAG CTTTCTGTCT TACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGAGC GGGCAGCTAA AGTTGCTGAG CAGCAGGAGA GAGAGCGAGC AGCACAGCAA 
CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG 
CCACCACCAA CACCAATGGG GATGCTCAAT CAGCAGTTGA CACCTGTTGC AGGCATGATG 
GGTGGCTATC CGCCAGGCCT TCCACCTTTG CAGGGCCCAG TTGATGGCCT TGTTAGCATG 
GGCAGCATGC AGCCACTTCA CCCTGGGGGG CCTCCACCTC ACCATCTTCC GCCAGGTGTG 
CCTGGCCTCC CAGGCATCCC ACCACCGGGT GTGATGAATC AAGGAGTAGC CCCCATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAACAGG TAGGAGTTTT GGGACCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGT CCTCATCCAG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CGCCCATGTT TGTGGCTCCC CCACCAAAGA CCCAAAGGCT TCTCCACTCA 
GAGGCCTACC TGAAATACAT TGAAGGACTC AGTGCTGAAT CCAACAGCAT TAGCAAGTGG 
GACCAAACTT TGGCAGCTCG AAGACGGGAT GTCCATTTGT CCAAAGAACA GGAGAGCCGC 
CTACCTTCTC ACTGGCTCAA AAGTAAAGGG GCACACACCA CCATGGCAGA TGCCCTCTGG 
CGCCTACGGG ATTTAATGCT TCGAGACACT CTCAACATCC GACAGGCATA CAACCTAGAA 
AATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171643.1
CDS	358..5406
Translation	MRRLAFRGAGCALVKLKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316154.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316154.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGTAAAA AGAACATAAG AAAACAGCGA ATGAAAATTT TATTCAATGT TGTTCTTGAA 
GCTCGAGAGC CAGGTTCAGG CAGAAGACTT TGCGATCTAT TTATGGTTAA GCCATCCAAG 
AAGGACTATC CTGATTATTA TAAAATCATC TTAGAGCCAA TGGACCTGAA AATAATTGAG 
CATAACATCC GAAATGACAA ATATGCAGGT GAAGAAGGAA TGATGGAAGA CATGAAACTC 
ATGTTCCGCA ATGCCAGGCA CTACAATGAG GAGGGCTCCC AGGTATACAA TGATGCCCAT 
ATCCTGGAGA AGTTACTCAA AGATAAAAGG AAAGAGCTGG GCCCTCTGCC TGATGATGAT 
GACATGGCTT CTCCCAAACT TAAATTGAGT AGGAAGAGTG GTGTTTCTCC TAAGAAATCA 
AAGTACATGA CTCCAATGCA GCAGAAACTG AATGAAGTGT ATGAAGCTGT AAAGAACTAT 
ACTGATAAGA GGGGTCGCCG CCTTAGTGCT ATATTTCTAA GACTCCCCTC TAGATCAGAG 
CTGCCTGACT ACTACCTGAC CATTAAAAAG CCCATGGACA TGGAAAAAAT TCGAAGTCAC 
ATGATGGCAA ACAAGTACCA AGACATAGAT TCTATGGTAG AGGACTTTGT CATGATGTTT 
AATAATGCCT GTACCTACAA TGAACCAGAG TCTTTGATCT ACAAAGATGC CCTTGTACTG 
CATAAAGTCC TCCTTGAGAC TCGGAGAGAC CTGGAGGGAG ATGAGGATTC TCATGTCCCT 
AATGTGACGT TGCTGATTCA AGAGCTCATC CATAACCTTT TTGTGTCAGT CATGAGTCAT 
CAGGATGACG AAGGGAGGTG TTACAGCGAC TCCTTAGCAG AAATTCCTGC TGTGGATCCC 
AACTCTCCCA ATAAACCTCC CCTTACATTT GACATTATCA GGAAAAATGT TGAAAGTAAT 
CGGTATCGGC GACTTGATTT ATTTCAGGAG CATATGTTTG AAGTATTGGA ACGGGCAAGA 
AGGATGAACC GGACAGATTC CGAAATATAT GAGGATGCTG TAGAACTTCA GCAGTTTTTT 
ATTAGAATTC GTGATGAACT CTGCAAAAAT GGAGAGATCC TTCTTTCTCC AGCACTCAGC 
TATACCACAA AACACTTGCA TAACGATGTG GAAAAAGAAA AAAAGGAAAA ATTGCCTAAA 
GAAATAGAGG AAGATAAACT AAAACGCGAA GAAGAAAAAA GAGAAGCTGA AAAAAGTGAA 
GATTCCTCAG GTACTACAGG CCTCTCAGGC TTACATCGTA CATACAGCCA GGACTGCAGC 
TTTAAGAACA GCATGTATCA TGTCGGAGAT TATGTCTATG TTGAACCTGC GGAGGCCAAT 
CTACAACCAC ATATAGTGTG TATTGAGAGA CTGTGGGAGG ATTCAGCTGG TGAAAAATGG 
TTGTACGGCT GTTGGTTTTA TCGGCCAAAT GAAACATTCC ATTTGGCTAC ACGAAAATTT 
CTAGAAAAAG AAGTTTTTAA GAGTGACTAC TACAATAAAG TACCTGTTAG TAAAATTCTA 
GGCAAATGTG TAGTCATGTT TGTCAAGGAA TACTTTAAAT TATGTCCAGA AAACTTTCGC 
GATGAGGATG TTTTTGTCTG TGAATCGAGG TATTCTGCCA AAACCAAATC TTTTAAGAAA 
ATTAAACTGT GGACCATGCC CATCAGTTCA GTTAGATTTG TCCCTCGGGA TGTGCCTTTG 
CCTGTGGTCC GAGTGGCCTC TGTGTTTGCA AATGCAGATA AAGGGGATGA TGAGAAGAAT 
ACAGACAACT CAGATGACAA TAGAGCTGAA GACAATTTTA ACTTGGAAAA GGAAAAAGAA 
GATGTTCCTG TGGAGATGTC CAATGGTGAG CCAGGTTGCC ACTACTTTGA GCAGCTTCGG 
TACAATGACA TGTGGCTGAA GGTTGGTGAT TGTGTCTTCA TCAAATCCCA CGGCTTGGTG 
CGCCCTCGTG TGGGCAGAAT TGAGAAAGTA TGGGTCCGAG ATGGAGCTGC ATATTTTTAT 
GGCCCTATCT TCATTCATCC AGAAGAAACA GAACATGAGC CCACAAAAAT GTTCTACAAA 
AAAGAAGTGT TTCTGAGTAA TCTGGAAGAG ACCTGCCCTA TGAGTTGTAT TCTGGGGAAA 
TGTGCAGTGC TGTCATTCAA GGACTTCCTC TCCTGCAGGC CAACTGAAAT ACCAGAAAAT 
GACATTCTGC TTTGTGAGAG CCGCTATAAT GAGAGTGACA AGCAGATGAA GAAGTTCAAG 
GGTTTGAAGA GGTTTTCACT CTCTGCTAAA GTTGTAGATG ATGAAATCTA CTACTTCAGA 
AAACCAATCA TTCCTCAGAA GGAACCCTCA CCTTTGTTAG AAAAGAAGAT ACAATTGCTA 
GAAGCTAAAT TTGCAGAGTT AGAAGGAGGA GATGATGATA TTGAGGAGAT GGGAGAAGAG 
GATAGTGAAG TCATTGAAGC TCCATCTCTA CCTCAACTGC AGACACCCCT GGCCAATGAG 
TTGGACCTCA TGCCCTATAC ACCCCCACAG CTTTCTGTCT TACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGAGC GGGCAGCTAA AGTTGCTGAG CAGCAGGAGA GAGAGCGAGC AGCACAGCAA 
CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG 
CCACCACCAA CACCAATGGG GATGCTCAAT CAGCAGTTGA CACCTGTTGC AGGCATGATG 
GGTGGCTATC CGCCAGGCCT TCCACCTTTG CAGGGCCCAG TTGATGGCCT TGTTAGCATG 
GGCAGCATGC AGCCACTTCA CCCTGGGGGG CCTCCACCTC ACCATCTTCC GCCAGGTGTG 
CCTGGCCTCC CAGGCATCCC ACCACCGGGT GTGATGAATC AAGGAGTAGC CCCCATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAACAGG TAGGAGTTTT GGGACCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGT CCTCATCCAG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CGCCCATGTT TGTGGCTCCC CCACCAAAGA CCCAAAGGCT TCTCCACTCA 
GAGGCCTACC TGAAATACAT TGAAGGACTC AGTGCTGAAT CCAACAGCAT TAGCAAGTGG 
GACCAAACTT TGGCAGCTCG AAGACGGGAT GTCCATTTGT CCAAAGAACA GGAGAGCCGC 
CTACCTTCTC ACTGGCTCAA AAGTAAAGGG GCACACACCA CCATGGCAGA TGCCCTCTGG 
CGCCTACGGG ATTTAATGCT TCGAGACACT CTCAACATCC GACAGGCATA CAACCTAGAA 
AATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316155.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171644.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X34, mRNA.

Price & Availability↑

CloneID	OMu78832
Clone ID Related Accession (Same CDS sequence)	XM_017316155.1
Accession Version	XM_017316155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGTAATAAA 
AGAGCAGTCC AAGGGGGTCG CTTGTCAGCA ATTACCATGG CACTTCAGTA TGGATCAGAG 
AGTGAAGAGG ACGCTGCTTT AGCTGCTGCA CGCTATGAAG AAGGGGAATC TGAAGCAGAG 
AGCATCACTT CCTTCATGGA CGTTTCCAAC CCCTTTCATC AGCTTTACGA CACAGTTAGG 
AGCTGTAGGA ATCACCAAGG GCAGCTCATA GCTGAACCTT TCTTCCATTT GCCTTCAAAG 
AAAAAATACC CAGATTATTA TCAGCAAATT AAAATGCCCA TATCACTTCA ACAGATCAGA 
ACAAAGCTAA AGAACCAAGA ATATGAAACT TTAGATCATT TGGAGTGTGA TCTGAATTTA 
ATGTTTGAAA ATGCCAAACG TTATAACGTT CCCAATTCAG CCATCTATAA GCGAGTTCTA 
AAACTGCAGC AAGTCATGCA GGCAAAGAAG AAGGAGCTTG CGAGGAGAGA TGACATTGAG 
GACGGAGACA GCATGATCTC CTCAGCCACT TCTGACACTG GTAGTGCCAA AAGGAAAAGT 
AAAAAGAACA TAAGAAAACA GCGAATGAAA ATTTTATTCA ATGTTGTTCT TGAAGCTCGA 
GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT CTATTTATGG TTAAGCCATC CAAGAAGGAC 
TATCCTGATT ATTATAAAAT CATCTTAGAG CCAATGGACC TGAAAATAAT TGAGCATAAC 
ATCCGAAATG ACAAATATGC AGGTGAAGAA GGAATGATGG AAGACATGAA ACTCATGTTC 
CGCAATGCCA GGCACTACAA TGAGGAGGGC TCCCAGGTAT ACAATGATGC CCATATCCTG 
GAGAAGTTAC TCAAAGATAA AAGGAAAGAG CTGGGCCCTC TGCCTGATGA TGATGACATG 
GCTTCTCCCA AACTTAAATT GAGTAGGAAG AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC 
ATGACTCCAA TGCAGCAGAA ACTGAATGAA GTGTATGAAG CTGTAAAGAA CTATACTGAT 
AAGAGGGGTC GCCGCCTTAG TGCTATATTT CTAAGACTCC CCTCTAGATC AGAGCTGCCT 
GACTACTACC TGACCATTAA AAAGCCCATG GACATGGAAA AAATTCGAAG TCACATGATG 
GCAAACAAGT ACCAAGACAT AGATTCTATG GTAGAGGACT TTGTCATGAT GTTTAATAAT 
GCCTGTACCT ACAATGAACC AGAGTCTTTG ATCTACAAAG ATGCCCTTGT ACTGCATAAA 
GTCCTCCTTG AGACTCGGAG AGACCTGGAG GGAGATGAGG ATTCTCATGT CCCTAATGTG 
ACGTTGCTGA TTCAAGAGCT CATCCATAAC CTTTTTGTGT CAGTCATGAG TCATCAGGAT 
GACGAAGGGA GGTGTTACAG CGACTCCTTA GCAGAAATTC CTGCTGTGGA TCCCAACTCT 
CCCAATAAAC CTCCCCTTAC ATTTGACATT ATCAGGAAAA ATGTTGAAAG TAATCGGTAT 
CGGCGACTTG ATTTATTTCA GGAGCATATG TTTGAAGTAT TGGAACGGGC AAGAAGGATG 
AACCGGACAG ATTCCGAAAT ATATGAGGAT GCTGTAGAAC TTCAGCAGTT TTTTATTAGA 
ATTCGTGATG AACTCTGCAA AAATGGAGAG ATCCTTCTTT CTCCAGCACT CAGCTATACC 
ACAAAACACT TGCATAACGA TGTGGAAAAA GAAAAAAAGG AAAAATTGCC TAAAGAAATA 
GAGGAAGATA AACTAAAACG CGAAGAAGAA AAAAGAGAAG CTGAAAAAAG TGAAGATTCC 
TCAGGTACTA CAGGCCTCTC AGGCTTACAT CGTACATACA GCCAGGACTG CAGCTTTAAG 
AACAGCATGT ATCATGTCGG AGATTATGTC TATGTTGAAC CTGCGGAGGC CAATCTACAA 
CCACATATAG TGTGTATTGA GAGACTGTGG GAGGATTCAG CTGGTGAAAA ATGGTTGTAC 
GGCTGTTGGT TTTATCGGCC AAATGAAACA TTCCATTTGG CTACACGAAA ATTTCTAGAA 
AAAGAAGTTT TTAAGAGTGA CTACTACAAT AAAGTACCTG TTAGTAAAAT TCTAGGCAAA 
TGTGTAGTCA TGTTTGTCAA GGAATACTTT AAATTATGTC CAGAAAACTT TCGCGATGAG 
GATGTTTTTG TCTGTGAATC GAGGTATTCT GCCAAAACCA AATCTTTTAA GAAAATTAAA 
CTGTGGACCA TGCCCATCAG TTCAGTTAGA TTTGTCCCTC GGGATGTGCC TTTGCCTGTG 
GTCCGAGTGG CCTCTGTGTT TGCAAATGCA GATAAAGGGG ATGATGAGAA GAATACAGAC 
AACTCAGATG ACAATAGAGC TGAAGACAAT TTTAACTTGG AAAAGGAAAA AGAAGATGTT 
CCTGTGGAGA TGTCCAATGG TGAGCCAGGT TGCCACTACT TTGAGCAGCT TCGGTACAAT 
GACATGTGGC TGAAGGTTGG TGATTGTGTC TTCATCAAAT CCCACGGCTT GGTGCGCCCT 
CGTGTGGGCA GAATTGAGAA AGTATGGGTC CGAGATGGAG CTGCATATTT TTATGGCCCT 
ATCTTCATTC ATCCAGAAGA AACAGAACAT GAGCCCACAA AAATGTTCTA CAAAAAAGAA 
GTGTTTCTGA GTAATCTGGA AGAGACCTGC CCTATGAGTT GTATTCTGGG GAAATGTGCA 
GTGCTGTCAT TCAAGGACTT CCTCTCCTGC AGGCCAACTG AAATACCAGA AAATGACATT 
CTGCTTTGTG AGAGCCGCTA TAATGAGAGT GACAAGCAGA TGAAGAAGTT CAAGGGTTTG 
AAGAGGTTTT CACTCTCTGC TAAAGTTGTA GATGATGAAA TCTACTACTT CAGAAAACCA 
ATCATTCCTC AGAAGGAACC CTCACCTTTG TTAGAAAAGA AGATACAATT GCTAGAAGCT 
AAATTTGCAG AGTTAGAAGG AGGAGATGAT GATATTGAGG AGATGGGAGA AGAGGATAGT 
GAAGTCATTG AAGCTCCATC TCTACCTCAA CTGCAGACAC CCCTGGCCAA TGAGTTGGAC 
CTCATGCCCT ATACACCCCC ACAGCTTTCT GTCTTACAGT CTACCCCAAA GTCTGCCAAA 
GGCAGTGCAA AGAAGGAAAG TTCTAAACGA AAAATCAACA TGAGTGGCTA CATTTTGTTC 
AGCAGTGAAA TGAGAGCTGT GATTAAAGCC CAGCACCCAG ACTACTCTTT TGGGGAGCTC 
AGCAGACTGG TGGGGACAGA ATGGAGAAAC CTTGAAACAG CCAAGAAAGC AGAATATGAA 
GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG GAGAGAGAGC GAGCAGCACA GCAACAGCAG 
CCGAGTGCTT CTCCCCGAGC AGGCACCCCT GTGGGGGCTC TCATGGGGGT GGTGCCACCA 
CCAACACCAA TGGGGATGCT CAATCAGCAG TTGACACCTG TTGCAGGCAT GATGGGTGGC 
TATCCGCCAG GCCTTCCACC TTTGCAGGGC CCAGTTGATG GCCTTGTTAG CATGGGCAGC 
ATGCAGCCAC TTCACCCTGG GGGGCCTCCA CCTCACCATC TTCCGCCAGG TGTGCCTGGC 
CTCCCAGGCA TCCCACCACC GGGTGTGATG AATCAAGGAG TAGCCCCCAT GGTAGGGACT 
CCAGCACCAG GTGGAAGTCC GTATGGACAA CAGGTAGGAG TTTTGGGACC TCCAGGGCAG 
CAGGCACCAC CTCCATATCC TGGTCCTCAT CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA 
ACAACGCCCA TGTTTGTGGC TCCCCCACCA AAGACCCAAA GGCTTCTCCA CTCAGAGGCC 
TACCTGAAAT ACATTGAAGG ACTCAGTGCT GAATCCAACA GCATTAGCAA GTGGGACCAA 
ACTTTGGCAG CTCGAAGACG GGATGTCCAT TTGTCCAAAG AACAGGAGAG CCGCCTACCT 
TCTCACTGGC TCAAAAGTAA AGGGGCACAC ACCACCATGG CAGATGCCCT CTGGCGCCTA 
CGGGATTTAA TGCTTCGAGA CACTCTCAAC ATCCGACAGG CATACAACCT AGAAAATGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171644.1
CDS	358..5400
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316155.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316155.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGTAATAAA 
AGAGCAGTCC AAGGGGGTCG CTTGTCAGCA ATTACCATGG CACTTCAGTA TGGATCAGAG 
AGTGAAGAGG ACGCTGCTTT AGCTGCTGCA CGCTATGAAG AAGGGGAATC TGAAGCAGAG 
AGCATCACTT CCTTCATGGA CGTTTCCAAC CCCTTTCATC AGCTTTACGA CACAGTTAGG 
AGCTGTAGGA ATCACCAAGG GCAGCTCATA GCTGAACCTT TCTTCCATTT GCCTTCAAAG 
AAAAAATACC CAGATTATTA TCAGCAAATT AAAATGCCCA TATCACTTCA ACAGATCAGA 
ACAAAGCTAA AGAACCAAGA ATATGAAACT TTAGATCATT TGGAGTGTGA TCTGAATTTA 
ATGTTTGAAA ATGCCAAACG TTATAACGTT CCCAATTCAG CCATCTATAA GCGAGTTCTA 
AAACTGCAGC AAGTCATGCA GGCAAAGAAG AAGGAGCTTG CGAGGAGAGA TGACATTGAG 
GACGGAGACA GCATGATCTC CTCAGCCACT TCTGACACTG GTAGTGCCAA AAGGAAAAGT 
AAAAAGAACA TAAGAAAACA GCGAATGAAA ATTTTATTCA ATGTTGTTCT TGAAGCTCGA 
GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT CTATTTATGG TTAAGCCATC CAAGAAGGAC 
TATCCTGATT ATTATAAAAT CATCTTAGAG CCAATGGACC TGAAAATAAT TGAGCATAAC 
ATCCGAAATG ACAAATATGC AGGTGAAGAA GGAATGATGG AAGACATGAA ACTCATGTTC 
CGCAATGCCA GGCACTACAA TGAGGAGGGC TCCCAGGTAT ACAATGATGC CCATATCCTG 
GAGAAGTTAC TCAAAGATAA AAGGAAAGAG CTGGGCCCTC TGCCTGATGA TGATGACATG 
GCTTCTCCCA AACTTAAATT GAGTAGGAAG AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC 
ATGACTCCAA TGCAGCAGAA ACTGAATGAA GTGTATGAAG CTGTAAAGAA CTATACTGAT 
AAGAGGGGTC GCCGCCTTAG TGCTATATTT CTAAGACTCC CCTCTAGATC AGAGCTGCCT 
GACTACTACC TGACCATTAA AAAGCCCATG GACATGGAAA AAATTCGAAG TCACATGATG 
GCAAACAAGT ACCAAGACAT AGATTCTATG GTAGAGGACT TTGTCATGAT GTTTAATAAT 
GCCTGTACCT ACAATGAACC AGAGTCTTTG ATCTACAAAG ATGCCCTTGT ACTGCATAAA 
GTCCTCCTTG AGACTCGGAG AGACCTGGAG GGAGATGAGG ATTCTCATGT CCCTAATGTG 
ACGTTGCTGA TTCAAGAGCT CATCCATAAC CTTTTTGTGT CAGTCATGAG TCATCAGGAT 
GACGAAGGGA GGTGTTACAG CGACTCCTTA GCAGAAATTC CTGCTGTGGA TCCCAACTCT 
CCCAATAAAC CTCCCCTTAC ATTTGACATT ATCAGGAAAA ATGTTGAAAG TAATCGGTAT 
CGGCGACTTG ATTTATTTCA GGAGCATATG TTTGAAGTAT TGGAACGGGC AAGAAGGATG 
AACCGGACAG ATTCCGAAAT ATATGAGGAT GCTGTAGAAC TTCAGCAGTT TTTTATTAGA 
ATTCGTGATG AACTCTGCAA AAATGGAGAG ATCCTTCTTT CTCCAGCACT CAGCTATACC 
ACAAAACACT TGCATAACGA TGTGGAAAAA GAAAAAAAGG AAAAATTGCC TAAAGAAATA 
GAGGAAGATA AACTAAAACG CGAAGAAGAA AAAAGAGAAG CTGAAAAAAG TGAAGATTCC 
TCAGGTACTA CAGGCCTCTC AGGCTTACAT CGTACATACA GCCAGGACTG CAGCTTTAAG 
AACAGCATGT ATCATGTCGG AGATTATGTC TATGTTGAAC CTGCGGAGGC CAATCTACAA 
CCACATATAG TGTGTATTGA GAGACTGTGG GAGGATTCAG CTGGTGAAAA ATGGTTGTAC 
GGCTGTTGGT TTTATCGGCC AAATGAAACA TTCCATTTGG CTACACGAAA ATTTCTAGAA 
AAAGAAGTTT TTAAGAGTGA CTACTACAAT AAAGTACCTG TTAGTAAAAT TCTAGGCAAA 
TGTGTAGTCA TGTTTGTCAA GGAATACTTT AAATTATGTC CAGAAAACTT TCGCGATGAG 
GATGTTTTTG TCTGTGAATC GAGGTATTCT GCCAAAACCA AATCTTTTAA GAAAATTAAA 
CTGTGGACCA TGCCCATCAG TTCAGTTAGA TTTGTCCCTC GGGATGTGCC TTTGCCTGTG 
GTCCGAGTGG CCTCTGTGTT TGCAAATGCA GATAAAGGGG ATGATGAGAA GAATACAGAC 
AACTCAGATG ACAATAGAGC TGAAGACAAT TTTAACTTGG AAAAGGAAAA AGAAGATGTT 
CCTGTGGAGA TGTCCAATGG TGAGCCAGGT TGCCACTACT TTGAGCAGCT TCGGTACAAT 
GACATGTGGC TGAAGGTTGG TGATTGTGTC TTCATCAAAT CCCACGGCTT GGTGCGCCCT 
CGTGTGGGCA GAATTGAGAA AGTATGGGTC CGAGATGGAG CTGCATATTT TTATGGCCCT 
ATCTTCATTC ATCCAGAAGA AACAGAACAT GAGCCCACAA AAATGTTCTA CAAAAAAGAA 
GTGTTTCTGA GTAATCTGGA AGAGACCTGC CCTATGAGTT GTATTCTGGG GAAATGTGCA 
GTGCTGTCAT TCAAGGACTT CCTCTCCTGC AGGCCAACTG AAATACCAGA AAATGACATT 
CTGCTTTGTG AGAGCCGCTA TAATGAGAGT GACAAGCAGA TGAAGAAGTT CAAGGGTTTG 
AAGAGGTTTT CACTCTCTGC TAAAGTTGTA GATGATGAAA TCTACTACTT CAGAAAACCA 
ATCATTCCTC AGAAGGAACC CTCACCTTTG TTAGAAAAGA AGATACAATT GCTAGAAGCT 
AAATTTGCAG AGTTAGAAGG AGGAGATGAT GATATTGAGG AGATGGGAGA AGAGGATAGT 
GAAGTCATTG AAGCTCCATC TCTACCTCAA CTGCAGACAC CCCTGGCCAA TGAGTTGGAC 
CTCATGCCCT ATACACCCCC ACAGCTTTCT GTCTTACAGT CTACCCCAAA GTCTGCCAAA 
GGCAGTGCAA AGAAGGAAAG TTCTAAACGA AAAATCAACA TGAGTGGCTA CATTTTGTTC 
AGCAGTGAAA TGAGAGCTGT GATTAAAGCC CAGCACCCAG ACTACTCTTT TGGGGAGCTC 
AGCAGACTGG TGGGGACAGA ATGGAGAAAC CTTGAAACAG CCAAGAAAGC AGAATATGAA 
GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG GAGAGAGAGC GAGCAGCACA GCAACAGCAG 
CCGAGTGCTT CTCCCCGAGC AGGCACCCCT GTGGGGGCTC TCATGGGGGT GGTGCCACCA 
CCAACACCAA TGGGGATGCT CAATCAGCAG TTGACACCTG TTGCAGGCAT GATGGGTGGC 
TATCCGCCAG GCCTTCCACC TTTGCAGGGC CCAGTTGATG GCCTTGTTAG CATGGGCAGC 
ATGCAGCCAC TTCACCCTGG GGGGCCTCCA CCTCACCATC TTCCGCCAGG TGTGCCTGGC 
CTCCCAGGCA TCCCACCACC GGGTGTGATG AATCAAGGAG TAGCCCCCAT GGTAGGGACT 
CCAGCACCAG GTGGAAGTCC GTATGGACAA CAGGTAGGAG TTTTGGGACC TCCAGGGCAG 
CAGGCACCAC CTCCATATCC TGGTCCTCAT CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA 
ACAACGCCCA TGTTTGTGGC TCCCCCACCA AAGACCCAAA GGCTTCTCCA CTCAGAGGCC 
TACCTGAAAT ACATTGAAGG ACTCAGTGCT GAATCCAACA GCATTAGCAA GTGGGACCAA 
ACTTTGGCAG CTCGAAGACG GGATGTCCAT TTGTCCAAAG AACAGGAGAG CCGCCTACCT 
TCTCACTGGC TCAAAAGTAA AGGGGCACAC ACCACCATGG CAGATGCCCT CTGGCGCCTA 
CGGGATTTAA TGCTTCGAGA CACTCTCAAC ATCCGACAGG CATACAACCT AGAAAATGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316156.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171645.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X40, mRNA.

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CloneID	OMu78833
Clone ID Related Accession (Same CDS sequence)	XM_017316156.1 , NM_001359854.1
Accession Version	XM_017316156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGAGC GGGCAGCTAA AGTTGCTGAG CAGCAGGAGA GAGAGCGAGC AGCACAGCAA 
CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG 
CCACCACCAA CACCAATGGG GATGCTCAAT CAGCAGTTGA CACCTGTTGC AGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171645.1
CDS	412..5424
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316156.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316156.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGAGC GGGCAGCTAA AGTTGCTGAG CAGCAGGAGA GAGAGCGAGC AGCACAGCAA 
CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG 
CCACCACCAA CACCAATGGG GATGCTCAAT CAGCAGTTGA CACCTGTTGC AGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316158.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171647.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X42, mRNA.

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CloneID	OMu78834
Clone ID Related Accession (Same CDS sequence)	XM_017316158.1 , XM_017316157.1
Accession Version	XM_017316158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGTAAAA AGAACATAAG AAAACAGCGA ATGAAAATTT TATTCAATGT TGTTCTTGAA 
GCTCGAGAGC CAGGTTCAGG CAGAAGACTT TGCGATCTAT TTATGGTTAA GCCATCCAAG 
AAGGACTATC CTGATTATTA TAAAATCATC TTAGAGCCAA TGGACCTGAA AATAATTGAG 
CATAACATCC GAAATGACAA ATATGCAGGT GAAGAAGGAA TGATGGAAGA CATGAAACTC 
ATGTTCCGCA ATGCCAGGCA CTACAATGAG GAGGGCTCCC AGGTATACAA TGATGCCCAT 
ATCCTGGAGA AGTTACTCAA AGATAAAAGG AAAGAGCTGG GCCCTCTGCC TGATGATGAT 
GACATGGCTT CTCCCAAACT TAAATTGAGT AGGAAGAGTG GTGTTTCTCC TAAGAAATCA 
AAGTACATGA CTCCAATGCA GCAGAAACTG AATGAAGTGT ATGAAGCTGT AAAGAACTAT 
ACTGATAAGA GGGGTCGCCG CCTTAGTGCT ATATTTCTAA GACTCCCCTC TAGATCAGAG 
CTGCCTGACT ACTACCTGAC CATTAAAAAG CCCATGGACA TGGAAAAAAT TCGAAGTCAC 
ATGATGGCAA ACAAGTACCA AGACATAGAT TCTATGGTAG AGGACTTTGT CATGATGTTT 
AATAATGCCT GTACCTACAA TGAACCAGAG TCTTTGATCT ACAAAGATGC CCTTGTACTG 
CATAAAGTCC TCCTTGAGAC TCGGAGAGAC CTGGAGGGAG ATGAGGATTC TCATGTCCCT 
AATGTGACGT TGCTGATTCA AGAGCTCATC CATAACCTTT TTGTGTCAGT CATGAGTCAT 
CAGGATGACG AAGGGAGGTG TTACAGCGAC TCCTTAGCAG AAATTCCTGC TGTGGATCCC 
AACTCTCCCA ATAAACCTCC CCTTACATTT GACATTATCA GGAAAAATGT TGAAAGTAAT 
CGGTATCGGC GACTTGATTT ATTTCAGGAG CATATGTTTG AAGTATTGGA ACGGGCAAGA 
AGGATGAACC GGACAGATTC CGAAATATAT GAGGATGCTG TAGAACTTCA GCAGTTTTTT 
ATTAGAATTC GTGATGAACT CTGCAAAAAT GGAGAGATCC TTCTTTCTCC AGCACTCAGC 
TATACCACAA AACACTTGCA TAACGATGTG GAAAAAGAAA AAAAGGAAAA ATTGCCTAAA 
GAAATAGAGG AAGATAAACT AAAACGCGAA GAAGAAAAAA GAGAAGCTGA AAAAAGTGAA 
GATTCCTCAG GTACTACAGG CCTCTCAGGC TTACATCGTA CATACAGCCA GGACTGCAGC 
TTTAAGAACA GCATGTATCA TGTCGGAGAT TATGTCTATG TTGAACCTGC GGAGGCCAAT 
CTACAACCAC ATATAGTGTG TATTGAGAGA CTGTGGGAGG ATTCAGCTGG TGAAAAATGG 
TTGTACGGCT GTTGGTTTTA TCGGCCAAAT GAAACATTCC ATTTGGCTAC ACGAAAATTT 
CTAGAAAAAG AAGTTTTTAA GAGTGACTAC TACAATAAAG TACCTGTTAG TAAAATTCTA 
GGCAAATGTG TAGTCATGTT TGTCAAGGAA TACTTTAAAT TATGTCCAGA AAACTTTCGC 
GATGAGGATG TTTTTGTCTG TGAATCGAGG TATTCTGCCA AAACCAAATC TTTTAAGAAA 
ATTAAACTGT GGACCATGCC CATCAGTTCA GTTAGATTTG TCCCTCGGGA TGTGCCTTTG 
CCTGTGGTCC GAGTGGCCTC TGTGTTTGCA AATGCAGATA AAGGGGATGA TGAGAAGAAT 
ACAGACAACT CAGATGACAA TAGAGCTGAA GACAATTTTA ACTTGGAAAA GGAAAAAGAA 
GATGTTCCTG TGGAGATGTC CAATGGTGAG CCAGGTTGCC ACTACTTTGA GCAGCTTCGG 
TACAATGACA TGTGGCTGAA GGTTGGTGAT TGTGTCTTCA TCAAATCCCA CGGCTTGGTG 
CGCCCTCGTG TGGGCAGAAT TGAGAAAGTA TGGGTCCGAG ATGGAGCTGC ATATTTTTAT 
GGCCCTATCT TCATTCATCC AGAAGAAACA GAACATGAGC CCACAAAAAT GTTCTACAAA 
AAAGAAGTGT TTCTGAGTAA TCTGGAAGAG ACCTGCCCTA TGAGTTGTAT TCTGGGGAAA 
TGTGCAGTGC TGTCATTCAA GGACTTCCTC TCCTGCAGGC CAACTGAAAT ACCAGAAAAT 
GACATTCTGC TTTGTGAGAG CCGCTATAAT GAGAGTGACA AGCAGATGAA GAAGTTCAAG 
GGTTTGAAGA GGTTTTCACT CTCTGCTAAA GTTGTAGATG ATGAAATCTA CTACTTCAGA 
AAACCAATCA TTCCTCAGAA GGAACCCTCA CCTTTGTTAG AAAAGAAGAT ACAATTGCTA 
GAAGCTAAAT TTGCAGAGTT AGAAGGAGGA GATGATGATA TTGAGGAGAT GGGAGAAGAG 
GATAGTGAAG TCATTGAAGC TCCATCTCTA CCTCAACTGC AGACACCCCT GGCCAATGAG 
TTGGACCTCA TGCCCTATAC ACCCCCACAG CTTTCTGTCT TACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGAGC GGGCAGCTAA AGTTGCTGAG CAGCAGGAGA GAGAGCGAGC AGCACAGCAA 
CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG 
CCACCACCAA CACCAATGGG GATGCTCAAT CAGCAGTTGA CACCTGTTGC AGGCATGATG 
GGTGGCTATC CGCCAGGCCT TCCACCTTTG CAGGGCCCAG TTGATGGCCT TGTTAGCATG 
GGCAGCATGC AGCCACTTCA CCCTGGGGGG CCTCCACCTC ACCATCTTCC GCCAGGTGTG 
CCTGGCCTCC CAGGCATCCC ACCACCGGGT GTGATGAATC AAGGAGTAGC CCCCATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAACAGG TAGGAGTTTT GGGACCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGT CCTCATCCAG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CGCCCATGTT TGTGGCTCCC CCACCAAAGA CCCAAAGGCT TCTCCACTCA 
GAGGCCTACC TGAAATACAT TGAAGGACTC AGTGCTGAAT CCAACAGCAT TAGCAAGTGG 
GACCAAACTT TGGCAGCTCG AAGACGGGAT GTCCATTTGT CCAAAGAACA GGAGAGCCGC 
CTACCTTCTC ACTGGCTCAA AAGTAAAGGG GCACACACCA CCATGGCAGA TGCCCTCTGG 
CGCCTACGGG ATTTAATGCT TCGAGACACT CTCAACATCC GACAGGCATA CAACCTAGAA 
AATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171647.1
CDS	305..5293
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316158.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X42, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316158.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGTAAAA AGAACATAAG AAAACAGCGA ATGAAAATTT TATTCAATGT TGTTCTTGAA 
GCTCGAGAGC CAGGTTCAGG CAGAAGACTT TGCGATCTAT TTATGGTTAA GCCATCCAAG 
AAGGACTATC CTGATTATTA TAAAATCATC TTAGAGCCAA TGGACCTGAA AATAATTGAG 
CATAACATCC GAAATGACAA ATATGCAGGT GAAGAAGGAA TGATGGAAGA CATGAAACTC 
ATGTTCCGCA ATGCCAGGCA CTACAATGAG GAGGGCTCCC AGGTATACAA TGATGCCCAT 
ATCCTGGAGA AGTTACTCAA AGATAAAAGG AAAGAGCTGG GCCCTCTGCC TGATGATGAT 
GACATGGCTT CTCCCAAACT TAAATTGAGT AGGAAGAGTG GTGTTTCTCC TAAGAAATCA 
AAGTACATGA CTCCAATGCA GCAGAAACTG AATGAAGTGT ATGAAGCTGT AAAGAACTAT 
ACTGATAAGA GGGGTCGCCG CCTTAGTGCT ATATTTCTAA GACTCCCCTC TAGATCAGAG 
CTGCCTGACT ACTACCTGAC CATTAAAAAG CCCATGGACA TGGAAAAAAT TCGAAGTCAC 
ATGATGGCAA ACAAGTACCA AGACATAGAT TCTATGGTAG AGGACTTTGT CATGATGTTT 
AATAATGCCT GTACCTACAA TGAACCAGAG TCTTTGATCT ACAAAGATGC CCTTGTACTG 
CATAAAGTCC TCCTTGAGAC TCGGAGAGAC CTGGAGGGAG ATGAGGATTC TCATGTCCCT 
AATGTGACGT TGCTGATTCA AGAGCTCATC CATAACCTTT TTGTGTCAGT CATGAGTCAT 
CAGGATGACG AAGGGAGGTG TTACAGCGAC TCCTTAGCAG AAATTCCTGC TGTGGATCCC 
AACTCTCCCA ATAAACCTCC CCTTACATTT GACATTATCA GGAAAAATGT TGAAAGTAAT 
CGGTATCGGC GACTTGATTT ATTTCAGGAG CATATGTTTG AAGTATTGGA ACGGGCAAGA 
AGGATGAACC GGACAGATTC CGAAATATAT GAGGATGCTG TAGAACTTCA GCAGTTTTTT 
ATTAGAATTC GTGATGAACT CTGCAAAAAT GGAGAGATCC TTCTTTCTCC AGCACTCAGC 
TATACCACAA AACACTTGCA TAACGATGTG GAAAAAGAAA AAAAGGAAAA ATTGCCTAAA 
GAAATAGAGG AAGATAAACT AAAACGCGAA GAAGAAAAAA GAGAAGCTGA AAAAAGTGAA 
GATTCCTCAG GTACTACAGG CCTCTCAGGC TTACATCGTA CATACAGCCA GGACTGCAGC 
TTTAAGAACA GCATGTATCA TGTCGGAGAT TATGTCTATG TTGAACCTGC GGAGGCCAAT 
CTACAACCAC ATATAGTGTG TATTGAGAGA CTGTGGGAGG ATTCAGCTGG TGAAAAATGG 
TTGTACGGCT GTTGGTTTTA TCGGCCAAAT GAAACATTCC ATTTGGCTAC ACGAAAATTT 
CTAGAAAAAG AAGTTTTTAA GAGTGACTAC TACAATAAAG TACCTGTTAG TAAAATTCTA 
GGCAAATGTG TAGTCATGTT TGTCAAGGAA TACTTTAAAT TATGTCCAGA AAACTTTCGC 
GATGAGGATG TTTTTGTCTG TGAATCGAGG TATTCTGCCA AAACCAAATC TTTTAAGAAA 
ATTAAACTGT GGACCATGCC CATCAGTTCA GTTAGATTTG TCCCTCGGGA TGTGCCTTTG 
CCTGTGGTCC GAGTGGCCTC TGTGTTTGCA AATGCAGATA AAGGGGATGA TGAGAAGAAT 
ACAGACAACT CAGATGACAA TAGAGCTGAA GACAATTTTA ACTTGGAAAA GGAAAAAGAA 
GATGTTCCTG TGGAGATGTC CAATGGTGAG CCAGGTTGCC ACTACTTTGA GCAGCTTCGG 
TACAATGACA TGTGGCTGAA GGTTGGTGAT TGTGTCTTCA TCAAATCCCA CGGCTTGGTG 
CGCCCTCGTG TGGGCAGAAT TGAGAAAGTA TGGGTCCGAG ATGGAGCTGC ATATTTTTAT 
GGCCCTATCT TCATTCATCC AGAAGAAACA GAACATGAGC CCACAAAAAT GTTCTACAAA 
AAAGAAGTGT TTCTGAGTAA TCTGGAAGAG ACCTGCCCTA TGAGTTGTAT TCTGGGGAAA 
TGTGCAGTGC TGTCATTCAA GGACTTCCTC TCCTGCAGGC CAACTGAAAT ACCAGAAAAT 
GACATTCTGC TTTGTGAGAG CCGCTATAAT GAGAGTGACA AGCAGATGAA GAAGTTCAAG 
GGTTTGAAGA GGTTTTCACT CTCTGCTAAA GTTGTAGATG ATGAAATCTA CTACTTCAGA 
AAACCAATCA TTCCTCAGAA GGAACCCTCA CCTTTGTTAG AAAAGAAGAT ACAATTGCTA 
GAAGCTAAAT TTGCAGAGTT AGAAGGAGGA GATGATGATA TTGAGGAGAT GGGAGAAGAG 
GATAGTGAAG TCATTGAAGC TCCATCTCTA CCTCAACTGC AGACACCCCT GGCCAATGAG 
TTGGACCTCA TGCCCTATAC ACCCCCACAG CTTTCTGTCT TACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGAGC GGGCAGCTAA AGTTGCTGAG CAGCAGGAGA GAGAGCGAGC AGCACAGCAA 
CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG 
CCACCACCAA CACCAATGGG GATGCTCAAT CAGCAGTTGA CACCTGTTGC AGGCATGATG 
GGTGGCTATC CGCCAGGCCT TCCACCTTTG CAGGGCCCAG TTGATGGCCT TGTTAGCATG 
GGCAGCATGC AGCCACTTCA CCCTGGGGGG CCTCCACCTC ACCATCTTCC GCCAGGTGTG 
CCTGGCCTCC CAGGCATCCC ACCACCGGGT GTGATGAATC AAGGAGTAGC CCCCATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAACAGG TAGGAGTTTT GGGACCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGT CCTCATCCAG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CGCCCATGTT TGTGGCTCCC CCACCAAAGA CCCAAAGGCT TCTCCACTCA 
GAGGCCTACC TGAAATACAT TGAAGGACTC AGTGCTGAAT CCAACAGCAT TAGCAAGTGG 
GACCAAACTT TGGCAGCTCG AAGACGGGAT GTCCATTTGT CCAAAGAACA GGAGAGCCGC 
CTACCTTCTC ACTGGCTCAA AAGTAAAGGG GCACACACCA CCATGGCAGA TGCCCTCTGG 
CGCCTACGGG ATTTAATGCT TCGAGACACT CTCAACATCC GACAGGCATA CAACCTAGAA 
AATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316157.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171646.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X41, mRNA.

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CloneID	OMu78834
Clone ID Related Accession (Same CDS sequence)	XM_017316158.1 , XM_017316157.1
Accession Version	XM_017316157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGTAAAA AGAACATAAG AAAACAGCGA ATGAAAATTT TATTCAATGT TGTTCTTGAA 
GCTCGAGAGC CAGGTTCAGG CAGAAGACTT TGCGATCTAT TTATGGTTAA GCCATCCAAG 
AAGGACTATC CTGATTATTA TAAAATCATC TTAGAGCCAA TGGACCTGAA AATAATTGAG 
CATAACATCC GAAATGACAA ATATGCAGGT GAAGAAGGAA TGATGGAAGA CATGAAACTC 
ATGTTCCGCA ATGCCAGGCA CTACAATGAG GAGGGCTCCC AGGTATACAA TGATGCCCAT 
ATCCTGGAGA AGTTACTCAA AGATAAAAGG AAAGAGCTGG GCCCTCTGCC TGATGATGAT 
GACATGGCTT CTCCCAAACT TAAATTGAGT AGGAAGAGTG GTGTTTCTCC TAAGAAATCA 
AAGTACATGA CTCCAATGCA GCAGAAACTG AATGAAGTGT ATGAAGCTGT AAAGAACTAT 
ACTGATAAGA GGGGTCGCCG CCTTAGTGCT ATATTTCTAA GACTCCCCTC TAGATCAGAG 
CTGCCTGACT ACTACCTGAC CATTAAAAAG CCCATGGACA TGGAAAAAAT TCGAAGTCAC 
ATGATGGCAA ACAAGTACCA AGACATAGAT TCTATGGTAG AGGACTTTGT CATGATGTTT 
AATAATGCCT GTACCTACAA TGAACCAGAG TCTTTGATCT ACAAAGATGC CCTTGTACTG 
CATAAAGTCC TCCTTGAGAC TCGGAGAGAC CTGGAGGGAG ATGAGGATTC TCATGTCCCT 
AATGTGACGT TGCTGATTCA AGAGCTCATC CATAACCTTT TTGTGTCAGT CATGAGTCAT 
CAGGATGACG AAGGGAGGTG TTACAGCGAC TCCTTAGCAG AAATTCCTGC TGTGGATCCC 
AACTCTCCCA ATAAACCTCC CCTTACATTT GACATTATCA GGAAAAATGT TGAAAGTAAT 
CGGTATCGGC GACTTGATTT ATTTCAGGAG CATATGTTTG AAGTATTGGA ACGGGCAAGA 
AGGATGAACC GGACAGATTC CGAAATATAT GAGGATGCTG TAGAACTTCA GCAGTTTTTT 
ATTAGAATTC GTGATGAACT CTGCAAAAAT GGAGAGATCC TTCTTTCTCC AGCACTCAGC 
TATACCACAA AACACTTGCA TAACGATGTG GAAAAAGAAA AAAAGGAAAA ATTGCCTAAA 
GAAATAGAGG AAGATAAACT AAAACGCGAA GAAGAAAAAA GAGAAGCTGA AAAAAGTGAA 
GATTCCTCAG GTACTACAGG CCTCTCAGGC TTACATCGTA CATACAGCCA GGACTGCAGC 
TTTAAGAACA GCATGTATCA TGTCGGAGAT TATGTCTATG TTGAACCTGC GGAGGCCAAT 
CTACAACCAC ATATAGTGTG TATTGAGAGA CTGTGGGAGG ATTCAGCTGG TGAAAAATGG 
TTGTACGGCT GTTGGTTTTA TCGGCCAAAT GAAACATTCC ATTTGGCTAC ACGAAAATTT 
CTAGAAAAAG AAGTTTTTAA GAGTGACTAC TACAATAAAG TACCTGTTAG TAAAATTCTA 
GGCAAATGTG TAGTCATGTT TGTCAAGGAA TACTTTAAAT TATGTCCAGA AAACTTTCGC 
GATGAGGATG TTTTTGTCTG TGAATCGAGG TATTCTGCCA AAACCAAATC TTTTAAGAAA 
ATTAAACTGT GGACCATGCC CATCAGTTCA GTTAGATTTG TCCCTCGGGA TGTGCCTTTG 
CCTGTGGTCC GAGTGGCCTC TGTGTTTGCA AATGCAGATA AAGGGGATGA TGAGAAGAAT 
ACAGACAACT CAGATGACAA TAGAGCTGAA GACAATTTTA ACTTGGAAAA GGAAAAAGAA 
GATGTTCCTG TGGAGATGTC CAATGGTGAG CCAGGTTGCC ACTACTTTGA GCAGCTTCGG 
TACAATGACA TGTGGCTGAA GGTTGGTGAT TGTGTCTTCA TCAAATCCCA CGGCTTGGTG 
CGCCCTCGTG TGGGCAGAAT TGAGAAAGTA TGGGTCCGAG ATGGAGCTGC ATATTTTTAT 
GGCCCTATCT TCATTCATCC AGAAGAAACA GAACATGAGC CCACAAAAAT GTTCTACAAA 
AAAGAAGTGT TTCTGAGTAA TCTGGAAGAG ACCTGCCCTA TGAGTTGTAT TCTGGGGAAA 
TGTGCAGTGC TGTCATTCAA GGACTTCCTC TCCTGCAGGC CAACTGAAAT ACCAGAAAAT 
GACATTCTGC TTTGTGAGAG CCGCTATAAT GAGAGTGACA AGCAGATGAA GAAGTTCAAG 
GGTTTGAAGA GGTTTTCACT CTCTGCTAAA GTTGTAGATG ATGAAATCTA CTACTTCAGA 
AAACCAATCA TTCCTCAGAA GGAACCCTCA CCTTTGTTAG AAAAGAAGAT ACAATTGCTA 
GAAGCTAAAT TTGCAGAGTT AGAAGGAGGA GATGATGATA TTGAGGAGAT GGGAGAAGAG 
GATAGTGAAG TCATTGAAGC TCCATCTCTA CCTCAACTGC AGACACCCCT GGCCAATGAG 
TTGGACCTCA TGCCCTATAC ACCCCCACAG CTTTCTGTCT TACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGAGC GGGCAGCTAA AGTTGCTGAG CAGCAGGAGA GAGAGCGAGC AGCACAGCAA 
CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG 
CCACCACCAA CACCAATGGG GATGCTCAAT CAGCAGTTGA CACCTGTTGC AGGCATGATG 
GGTGGCTATC CGCCAGGCCT TCCACCTTTG CAGGGCCCAG TTGATGGCCT TGTTAGCATG 
GGCAGCATGC AGCCACTTCA CCCTGGGGGG CCTCCACCTC ACCATCTTCC GCCAGGTGTG 
CCTGGCCTCC CAGGCATCCC ACCACCGGGT GTGATGAATC AAGGAGTAGC CCCCATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAACAGG TAGGAGTTTT GGGACCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGT CCTCATCCAG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CGCCCATGTT TGTGGCTCCC CCACCAAAGA CCCAAAGGCT TCTCCACTCA 
GAGGCCTACC TGAAATACAT TGAAGGACTC AGTGCTGAAT CCAACAGCAT TAGCAAGTGG 
GACCAAACTT TGGCAGCTCG AAGACGGGAT GTCCATTTGT CCAAAGAACA GGAGAGCCGC 
CTACCTTCTC ACTGGCTCAA AAGTAAAGGG GCACACACCA CCATGGCAGA TGCCCTCTGG 
CGCCTACGGG ATTTAATGCT TCGAGACACT CTCAACATCC GACAGGCATA CAACCTAGAA 
AATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171646.1
CDS	308..5296
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316157.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316157.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGTAAAA AGAACATAAG AAAACAGCGA ATGAAAATTT TATTCAATGT TGTTCTTGAA 
GCTCGAGAGC CAGGTTCAGG CAGAAGACTT TGCGATCTAT TTATGGTTAA GCCATCCAAG 
AAGGACTATC CTGATTATTA TAAAATCATC TTAGAGCCAA TGGACCTGAA AATAATTGAG 
CATAACATCC GAAATGACAA ATATGCAGGT GAAGAAGGAA TGATGGAAGA CATGAAACTC 
ATGTTCCGCA ATGCCAGGCA CTACAATGAG GAGGGCTCCC AGGTATACAA TGATGCCCAT 
ATCCTGGAGA AGTTACTCAA AGATAAAAGG AAAGAGCTGG GCCCTCTGCC TGATGATGAT 
GACATGGCTT CTCCCAAACT TAAATTGAGT AGGAAGAGTG GTGTTTCTCC TAAGAAATCA 
AAGTACATGA CTCCAATGCA GCAGAAACTG AATGAAGTGT ATGAAGCTGT AAAGAACTAT 
ACTGATAAGA GGGGTCGCCG CCTTAGTGCT ATATTTCTAA GACTCCCCTC TAGATCAGAG 
CTGCCTGACT ACTACCTGAC CATTAAAAAG CCCATGGACA TGGAAAAAAT TCGAAGTCAC 
ATGATGGCAA ACAAGTACCA AGACATAGAT TCTATGGTAG AGGACTTTGT CATGATGTTT 
AATAATGCCT GTACCTACAA TGAACCAGAG TCTTTGATCT ACAAAGATGC CCTTGTACTG 
CATAAAGTCC TCCTTGAGAC TCGGAGAGAC CTGGAGGGAG ATGAGGATTC TCATGTCCCT 
AATGTGACGT TGCTGATTCA AGAGCTCATC CATAACCTTT TTGTGTCAGT CATGAGTCAT 
CAGGATGACG AAGGGAGGTG TTACAGCGAC TCCTTAGCAG AAATTCCTGC TGTGGATCCC 
AACTCTCCCA ATAAACCTCC CCTTACATTT GACATTATCA GGAAAAATGT TGAAAGTAAT 
CGGTATCGGC GACTTGATTT ATTTCAGGAG CATATGTTTG AAGTATTGGA ACGGGCAAGA 
AGGATGAACC GGACAGATTC CGAAATATAT GAGGATGCTG TAGAACTTCA GCAGTTTTTT 
ATTAGAATTC GTGATGAACT CTGCAAAAAT GGAGAGATCC TTCTTTCTCC AGCACTCAGC 
TATACCACAA AACACTTGCA TAACGATGTG GAAAAAGAAA AAAAGGAAAA ATTGCCTAAA 
GAAATAGAGG AAGATAAACT AAAACGCGAA GAAGAAAAAA GAGAAGCTGA AAAAAGTGAA 
GATTCCTCAG GTACTACAGG CCTCTCAGGC TTACATCGTA CATACAGCCA GGACTGCAGC 
TTTAAGAACA GCATGTATCA TGTCGGAGAT TATGTCTATG TTGAACCTGC GGAGGCCAAT 
CTACAACCAC ATATAGTGTG TATTGAGAGA CTGTGGGAGG ATTCAGCTGG TGAAAAATGG 
TTGTACGGCT GTTGGTTTTA TCGGCCAAAT GAAACATTCC ATTTGGCTAC ACGAAAATTT 
CTAGAAAAAG AAGTTTTTAA GAGTGACTAC TACAATAAAG TACCTGTTAG TAAAATTCTA 
GGCAAATGTG TAGTCATGTT TGTCAAGGAA TACTTTAAAT TATGTCCAGA AAACTTTCGC 
GATGAGGATG TTTTTGTCTG TGAATCGAGG TATTCTGCCA AAACCAAATC TTTTAAGAAA 
ATTAAACTGT GGACCATGCC CATCAGTTCA GTTAGATTTG TCCCTCGGGA TGTGCCTTTG 
CCTGTGGTCC GAGTGGCCTC TGTGTTTGCA AATGCAGATA AAGGGGATGA TGAGAAGAAT 
ACAGACAACT CAGATGACAA TAGAGCTGAA GACAATTTTA ACTTGGAAAA GGAAAAAGAA 
GATGTTCCTG TGGAGATGTC CAATGGTGAG CCAGGTTGCC ACTACTTTGA GCAGCTTCGG 
TACAATGACA TGTGGCTGAA GGTTGGTGAT TGTGTCTTCA TCAAATCCCA CGGCTTGGTG 
CGCCCTCGTG TGGGCAGAAT TGAGAAAGTA TGGGTCCGAG ATGGAGCTGC ATATTTTTAT 
GGCCCTATCT TCATTCATCC AGAAGAAACA GAACATGAGC CCACAAAAAT GTTCTACAAA 
AAAGAAGTGT TTCTGAGTAA TCTGGAAGAG ACCTGCCCTA TGAGTTGTAT TCTGGGGAAA 
TGTGCAGTGC TGTCATTCAA GGACTTCCTC TCCTGCAGGC CAACTGAAAT ACCAGAAAAT 
GACATTCTGC TTTGTGAGAG CCGCTATAAT GAGAGTGACA AGCAGATGAA GAAGTTCAAG 
GGTTTGAAGA GGTTTTCACT CTCTGCTAAA GTTGTAGATG ATGAAATCTA CTACTTCAGA 
AAACCAATCA TTCCTCAGAA GGAACCCTCA CCTTTGTTAG AAAAGAAGAT ACAATTGCTA 
GAAGCTAAAT TTGCAGAGTT AGAAGGAGGA GATGATGATA TTGAGGAGAT GGGAGAAGAG 
GATAGTGAAG TCATTGAAGC TCCATCTCTA CCTCAACTGC AGACACCCCT GGCCAATGAG 
TTGGACCTCA TGCCCTATAC ACCCCCACAG CTTTCTGTCT TACAGTCTAC CCCAAAGTCT 
GCCAAAGGCA GTGCAAAGAA GGAAAGTTCT AAACGAAAAA TCAACATGAG TGGCTACATT 
TTGTTCAGCA GTGAAATGAG AGCTGTGATT AAAGCCCAGC ACCCAGACTA CTCTTTTGGG 
GAGCTCAGCA GACTGGTGGG GACAGAATGG AGAAACCTTG AAACAGCCAA GAAAGCAGAA 
TATGAAGAGC GGGCAGCTAA AGTTGCTGAG CAGCAGGAGA GAGAGCGAGC AGCACAGCAA 
CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG 
CCACCACCAA CACCAATGGG GATGCTCAAT CAGCAGTTGA CACCTGTTGC AGGCATGATG 
GGTGGCTATC CGCCAGGCCT TCCACCTTTG CAGGGCCCAG TTGATGGCCT TGTTAGCATG 
GGCAGCATGC AGCCACTTCA CCCTGGGGGG CCTCCACCTC ACCATCTTCC GCCAGGTGTG 
CCTGGCCTCC CAGGCATCCC ACCACCGGGT GTGATGAATC AAGGAGTAGC CCCCATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAACAGG TAGGAGTTTT GGGACCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGT CCTCATCCAG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CGCCCATGTT TGTGGCTCCC CCACCAAAGA CCCAAAGGCT TCTCCACTCA 
GAGGCCTACC TGAAATACAT TGAAGGACTC AGTGCTGAAT CCAACAGCAT TAGCAAGTGG 
GACCAAACTT TGGCAGCTCG AAGACGGGAT GTCCATTTGT CCAAAGAACA GGAGAGCCGC 
CTACCTTCTC ACTGGCTCAA AAGTAAAGGG GCACACACCA CCATGGCAGA TGCCCTCTGG 
CGCCTACGGG ATTTAATGCT TCGAGACACT CTCAACATCC GACAGGCATA CAACCTAGAA 
AATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316159.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171648.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X44, mRNA.

Price & Availability↑

CloneID	OMu78835
Clone ID Related Accession (Same CDS sequence)	XM_017316159.1
Accession Version	XM_017316159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GTAAAAAGAA CATAAGAAAA 
CAGCGAATGA AAATTTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGCG ATCTATTTAT GGTTAAGCCA TCCAAGAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTAG AGCCAATGGA CCTGAAAATA ATTGAGCATA ACATCCGAAA TGACAAATAT 
GCAGGTGAAG AAGGAATGAT GGAAGACATG AAACTCATGT TCCGCAATGC CAGGCACTAC 
AATGAGGAGG GCTCCCAGGT ATACAATGAT GCCCATATCC TGGAGAAGTT ACTCAAAGAT 
AAAAGGAAAG AGCTGGGCCC TCTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTTAAA 
TTGAGTAGGA AGAGTGGTGT TTCTCCTAAG AAATCAAAGT ACATGACTCC AATGCAGCAG 
AAACTGAATG AAGTGTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTT 
AGTGCTATAT TTCTAAGACT CCCCTCTAGA TCAGAGCTGC CTGACTACTA CCTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCAAACAA GTACCAAGAC 
ATAGATTCTA TGGTAGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC CTACAATGAA 
CCAGAGTCTT TGATCTACAA AGATGCCCTT GTACTGCATA AAGTCCTCCT TGAGACTCGG 
AGAGACCTGG AGGGAGATGA GGATTCTCAT GTCCCTAATG TGACGTTGCT GATTCAAGAG 
CTCATCCATA ACCTTTTTGT GTCAGTCATG AGTCATCAGG ATGACGAAGG GAGGTGTTAC 
AGCGACTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAACT CTCCCAATAA ACCTCCCCTT 
ACATTTGACA TTATCAGGAA AAATGTTGAA AGTAATCGGT ATCGGCGACT TGATTTATTT 
CAGGAGCATA TGTTTGAAGT ATTGGAACGG GCAAGAAGGA TGAACCGGAC AGATTCCGAA 
ATATATGAGG ATGCTGTAGA ACTTCAGCAG TTTTTTATTA GAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCTCCAGCA CTCAGCTATA CCACAAAACA CTTGCATAAC 
GATGTGGAAA AAGAAAAAAA GGAAAAATTG CCTAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGCGAAGAAG AAAAAAGAGA AGCTGAAAAA AGTGAAGATT CCTCAGGTAC TACAGGCCTC 
TCAGGCTTAC ATCGTACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTATCATGTC 
GGAGATTATG TCTATGTTGA ACCTGCGGAG GCCAATCTAC AACCACATAT AGTGTGTATT 
GAGAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ACGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTACTACA ATAAAGTACC TGTTAGTAAA ATTCTAGGCA AATGTGTAGT CATGTTTGTC 
AAGGAATACT TTAAATTATG TCCAGAAAAC TTTCGCGATG AGGATGTTTT TGTCTGTGAA 
TCGAGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGTTCAGTTA GATTTGTCCC TCGGGATGTG CCTTTGCCTG TGGTCCGAGT GGCCTCTGTG 
TTTGCAAATG CAGATAAAGG GGATGATGAG AAGAATACAG ACAACTCAGA TGACAATAGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TTCCTGTGGA GATGTCCAAT 
GGTGAGCCAG GTTGCCACTA CTTTGAGCAG CTTCGGTACA ATGACATGTG GCTGAAGGTT 
GGTGATTGTG TCTTCATCAA ATCCCACGGC TTGGTGCGCC CTCGTGTGGG CAGAATTGAG 
AAAGTATGGG TCCGAGATGG AGCTGCATAT TTTTATGGCC CTATCTTCAT TCATCCAGAA 
GAAACAGAAC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTGTTTCT GAGTAATCTG 
GAAGAGACCT GCCCTATGAG TTGTATTCTG GGGAAATGTG CAGTGCTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TATAATGAGA GTGACAAGCA GATGAAGAAG TTCAAGGGTT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTTG TAGATGATGA AATCTACTAC TTCAGAAAAC CAATCATTCC TCAGAAGGAA 
CCCTCACCTT TGTTAGAAAA GAAGATACAA TTGCTAGAAG CTAAATTTGC AGAGTTAGAA 
GGAGGAGATG ATGATATTGA GGAGATGGGA GAAGAGGATA GTGAAGTCAT TGAAGCTCCA 
TCTCTACCTC AACTGCAGAC ACCCCTGGCC AATGAGTTGG ACCTCATGCC CTATACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGTGTGAT GAATCAAGGA GTAGCCCCCA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CGTATGGACA ACAGGTAGGA GTTTTGGGAC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CTGGTCCTCA TCCAGCTGGC CCCCCTGTCA TACAGCAGCC AACAACGCCC 
ATGTTTGTGG CTCCCCCACC AAAGACCCAA AGGCTTCTCC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC TGAATCCAAC AGCATTAGCA AGTGGGACCA AACTTTGGCA 
GCTCGAAGAC GGGATGTCCA TTTGTCCAAA GAACAGGAGA GCCGCCTACC TTCTCACTGG 
CTCAAAAGTA AAGGGGCACA CACCACCATG GCAGATGCCC TCTGGCGCCT ACGGGATTTA 
ATGCTTCGAG ACACTCTCAA CATCCGACAG GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171648.1
CDS	312..5225
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316159.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X44, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316159.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GTAAAAAGAA CATAAGAAAA 
CAGCGAATGA AAATTTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGCG ATCTATTTAT GGTTAAGCCA TCCAAGAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTAG AGCCAATGGA CCTGAAAATA ATTGAGCATA ACATCCGAAA TGACAAATAT 
GCAGGTGAAG AAGGAATGAT GGAAGACATG AAACTCATGT TCCGCAATGC CAGGCACTAC 
AATGAGGAGG GCTCCCAGGT ATACAATGAT GCCCATATCC TGGAGAAGTT ACTCAAAGAT 
AAAAGGAAAG AGCTGGGCCC TCTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTTAAA 
TTGAGTAGGA AGAGTGGTGT TTCTCCTAAG AAATCAAAGT ACATGACTCC AATGCAGCAG 
AAACTGAATG AAGTGTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTT 
AGTGCTATAT TTCTAAGACT CCCCTCTAGA TCAGAGCTGC CTGACTACTA CCTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCAAACAA GTACCAAGAC 
ATAGATTCTA TGGTAGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC CTACAATGAA 
CCAGAGTCTT TGATCTACAA AGATGCCCTT GTACTGCATA AAGTCCTCCT TGAGACTCGG 
AGAGACCTGG AGGGAGATGA GGATTCTCAT GTCCCTAATG TGACGTTGCT GATTCAAGAG 
CTCATCCATA ACCTTTTTGT GTCAGTCATG AGTCATCAGG ATGACGAAGG GAGGTGTTAC 
AGCGACTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAACT CTCCCAATAA ACCTCCCCTT 
ACATTTGACA TTATCAGGAA AAATGTTGAA AGTAATCGGT ATCGGCGACT TGATTTATTT 
CAGGAGCATA TGTTTGAAGT ATTGGAACGG GCAAGAAGGA TGAACCGGAC AGATTCCGAA 
ATATATGAGG ATGCTGTAGA ACTTCAGCAG TTTTTTATTA GAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCTCCAGCA CTCAGCTATA CCACAAAACA CTTGCATAAC 
GATGTGGAAA AAGAAAAAAA GGAAAAATTG CCTAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGCGAAGAAG AAAAAAGAGA AGCTGAAAAA AGTGAAGATT CCTCAGGTAC TACAGGCCTC 
TCAGGCTTAC ATCGTACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTATCATGTC 
GGAGATTATG TCTATGTTGA ACCTGCGGAG GCCAATCTAC AACCACATAT AGTGTGTATT 
GAGAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ACGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTACTACA ATAAAGTACC TGTTAGTAAA ATTCTAGGCA AATGTGTAGT CATGTTTGTC 
AAGGAATACT TTAAATTATG TCCAGAAAAC TTTCGCGATG AGGATGTTTT TGTCTGTGAA 
TCGAGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGTTCAGTTA GATTTGTCCC TCGGGATGTG CCTTTGCCTG TGGTCCGAGT GGCCTCTGTG 
TTTGCAAATG CAGATAAAGG GGATGATGAG AAGAATACAG ACAACTCAGA TGACAATAGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TTCCTGTGGA GATGTCCAAT 
GGTGAGCCAG GTTGCCACTA CTTTGAGCAG CTTCGGTACA ATGACATGTG GCTGAAGGTT 
GGTGATTGTG TCTTCATCAA ATCCCACGGC TTGGTGCGCC CTCGTGTGGG CAGAATTGAG 
AAAGTATGGG TCCGAGATGG AGCTGCATAT TTTTATGGCC CTATCTTCAT TCATCCAGAA 
GAAACAGAAC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTGTTTCT GAGTAATCTG 
GAAGAGACCT GCCCTATGAG TTGTATTCTG GGGAAATGTG CAGTGCTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TATAATGAGA GTGACAAGCA GATGAAGAAG TTCAAGGGTT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTTG TAGATGATGA AATCTACTAC TTCAGAAAAC CAATCATTCC TCAGAAGGAA 
CCCTCACCTT TGTTAGAAAA GAAGATACAA TTGCTAGAAG CTAAATTTGC AGAGTTAGAA 
GGAGGAGATG ATGATATTGA GGAGATGGGA GAAGAGGATA GTGAAGTCAT TGAAGCTCCA 
TCTCTACCTC AACTGCAGAC ACCCCTGGCC AATGAGTTGG ACCTCATGCC CTATACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGTGTGAT GAATCAAGGA GTAGCCCCCA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CGTATGGACA ACAGGTAGGA GTTTTGGGAC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CTGGTCCTCA TCCAGCTGGC CCCCCTGTCA TACAGCAGCC AACAACGCCC 
ATGTTTGTGG CTCCCCCACC AAAGACCCAA AGGCTTCTCC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC TGAATCCAAC AGCATTAGCA AGTGGGACCA AACTTTGGCA 
GCTCGAAGAC GGGATGTCCA TTTGTCCAAA GAACAGGAGA GCCGCCTACC TTCTCACTGG 
CTCAAAAGTA AAGGGGCACA CACCACCATG GCAGATGCCC TCTGGCGCCT ACGGGATTTA 
ATGCTTCGAG ACACTCTCAA CATCCGACAG GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316160.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171649.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X49, mRNA.

Price & Availability↑

CloneID	OMu78836
Clone ID Related Accession (Same CDS sequence)	NM_001359857.1 , XM_017316160.1 , NM_001359858.1
Accession Version	XM_017316160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGGA ATACTCATGA CAGTGAGATG TTGGGTCTCA GGAGGCTATC CAGTAAAAAG 
AACATAAGAA AACAGCGAAT GAAAATTTTA TTCAATGTTG TTCTTGAAGC TCGAGAGCCA 
GGTTCAGGCA GAAGACTTTG CGATCTATTT ATGGTTAAGC CATCCAAGAA GGACTATCCT 
GATTATTATA AAATCATCTT AGAGCCAATG GACCTGAAAA TAATTGAGCA TAACATCCGA 
AATGACAAAT ATGCAGGTGA AGAAGGAATG ATGGAAGACA TGAAACTCAT GTTCCGCAAT 
GCCAGGCACT ACAATGAGGA GGGCTCCCAG GTATACAATG ATGCCCATAT CCTGGAGAAG 
TTACTCAAAG ATAAAAGGAA AGAGCTGGGC CCTCTGCCTG ATGATGATGA CATGGCTTCT 
CCCAAACTTA AATTGAGTAG GAAGAGTGGT GTTTCTCCTA AGAAATCAAA GTACATGACT 
CCAATGCAGC AGAAACTGAA TGAAGTGTAT GAAGCTGTAA AGAACTATAC TGATAAGAGG 
GGTCGCCGCC TTAGTGCTAT ATTTCTAAGA CTCCCCTCTA GATCAGAGCT GCCTGACTAC 
TACCTGACCA TTAAAAAGCC CATGGACATG GAAAAAATTC GAAGTCACAT GATGGCAAAC 
AAGTACCAAG ACATAGATTC TATGGTAGAG GACTTTGTCA TGATGTTTAA TAATGCCTGT 
ACCTACAATG AACCAGAGTC TTTGATCTAC AAAGATGCCC TTGTACTGCA TAAAGTCCTC 
CTTGAGACTC GGAGAGACCT GGAGGGAGAT GAGGATTCTC ATGTCCCTAA TGTGACGTTG 
CTGATTCAAG AGCTCATCCA TAACCTTTTT GTGTCAGTCA TGAGTCATCA GGATGACGAA 
GGGAGGTGTT ACAGCGACTC CTTAGCAGAA ATTCCTGCTG TGGATCCCAA CTCTCCCAAT 
AAACCTCCCC TTACATTTGA CATTATCAGG AAAAATGTTG AAAGTAATCG GTATCGGCGA 
CTTGATTTAT TTCAGGAGCA TATGTTTGAA GTATTGGAAC GGGCAAGAAG GATGAACCGG 
ACAGATTCCG AAATATATGA GGATGCTGTA GAACTTCAGC AGTTTTTTAT TAGAATTCGT 
GATGAACTCT GCAAAAATGG AGAGATCCTT CTTTCTCCAG CACTCAGCTA TACCACAAAA 
CACTTGCATA ACGATGTGGA AAAAGAAAAA AAGGAAAAAT TGCCTAAAGA AATAGAGGAA 
GATAAACTAA AACGCGAAGA AGAAAAAAGA GAAGCTGAAA AAAGTGAAGA TTCCTCAGGT 
ACTACAGGCC TCTCAGGCTT ACATCGTACA TACAGCCAGG ACTGCAGCTT TAAGAACAGC 
ATGTATCATG TCGGAGATTA TGTCTATGTT GAACCTGCGG AGGCCAATCT ACAACCACAT 
ATAGTGTGTA TTGAGAGACT GTGGGAGGAT TCAGCTGGTG AAAAATGGTT GTACGGCTGT 
TGGTTTTATC GGCCAAATGA AACATTCCAT TTGGCTACAC GAAAATTTCT AGAAAAAGAA 
GTTTTTAAGA GTGACTACTA CAATAAAGTA CCTGTTAGTA AAATTCTAGG CAAATGTGTA 
GTCATGTTTG TCAAGGAATA CTTTAAATTA TGTCCAGAAA ACTTTCGCGA TGAGGATGTT 
TTTGTCTGTG AATCGAGGTA TTCTGCCAAA ACCAAATCTT TTAAGAAAAT TAAACTGTGG 
ACCATGCCCA TCAGTTCAGT TAGATTTGTC CCTCGGGATG TGCCTTTGCC TGTGGTCCGA 
GTGGCCTCTG TGTTTGCAAA TGCAGATAAA GGGGATGATG AGAAGAATAC AGACAACTCA 
GATGACAATA GAGCTGAAGA CAATTTTAAC TTGGAAAAGG AAAAAGAAGA TGTTCCTGTG 
GAGATGTCCA ATGGTGAGCC AGGTTGCCAC TACTTTGAGC AGCTTCGGTA CAATGACATG 
TGGCTGAAGG TTGGTGATTG TGTCTTCATC AAATCCCACG GCTTGGTGCG CCCTCGTGTG 
GGCAGAATTG AGAAAGTATG GGTCCGAGAT GGAGCTGCAT ATTTTTATGG CCCTATCTTC 
ATTCATCCAG AAGAAACAGA ACATGAGCCC ACAAAAATGT TCTACAAAAA AGAAGTGTTT 
CTGAGTAATC TGGAAGAGAC CTGCCCTATG AGTTGTATTC TGGGGAAATG TGCAGTGCTG 
TCATTCAAGG ACTTCCTCTC CTGCAGGCCA ACTGAAATAC CAGAAAATGA CATTCTGCTT 
TGTGAGAGCC GCTATAATGA GAGTGACAAG CAGATGAAGA AGTTCAAGGG TTTGAAGAGG 
TTTTCACTCT CTGCTAAAGT TGTAGATGAT GAAATCTACT ACTTCAGAAA ACCAATCATT 
CCTCAGAAGG AACCCTCACC TTTGTTAGAA AAGAAGATAC AATTGCTAGA AGCTAAATTT 
GCAGAGTTAG AAGGAGGAGA TGATGATATT GAGGAGATGG GAGAAGAGGA TAGTGAAGTC 
ATTGAAGCTC CATCTCTACC TCAACTGCAG ACACCCCTGG CCAATGAGTT GGACCTCATG 
CCCTATACAC CCCCACAGTC TACCCCAAAG TCTGCCAAAG GCAGTGCAAA GAAGGAAAGT 
TCTAAACGAA AAATCAACAT GAGTGGCTAC ATTTTGTTCA GCAGTGAAAT GAGAGCTGTG 
ATTAAAGCCC AGCACCCAGA CTACTCTTTT GGGGAGCTCA GCAGACTGGT GGGGACAGAA 
TGGAGAAACC TTGAAACAGC CAAGAAAGCA GAATATGAAG GTGTGATGAA TCAAGGAGTA 
GCCCCCATGG TAGGGACTCC AGCACCAGGT GGAAGTCCGT ATGGACAACA GGTAGGAGTT 
TTGGGACCTC CAGGGCAGCA GGCACCACCT CCATATCCTG GTCCTCATCC AGCTGGCCCC 
CCTGTCATAC AGCAGCCAAC AACGCCCATG TTTGTGGCTC CCCCACCAAA GACCCAAAGG 
CTTCTCCACT CAGAGGCCTA CCTGAAATAC ATTGAAGGAC TCAGTGCTGA ATCCAACAGC 
ATTAGCAAGT GGGACCAAAC TTTGGCAGCT CGAAGACGGG ATGTCCATTT GTCCAAAGAA 
CAGGAGAGCC GCCTACCTTC TCACTGGCTC AAAAGTAAAG GGGCACACAC CACCATGGCA 
GATGCCCTCT GGCGCCTACG GGATTTAATG CTTCGAGACA CTCTCAACAT CCGACAGGCA 
TACAACCTAG AAAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171649.1
CDS	359..5119
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316160.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X49, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316160.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGGA ATACTCATGA CAGTGAGATG TTGGGTCTCA GGAGGCTATC CAGTAAAAAG 
AACATAAGAA AACAGCGAAT GAAAATTTTA TTCAATGTTG TTCTTGAAGC TCGAGAGCCA 
GGTTCAGGCA GAAGACTTTG CGATCTATTT ATGGTTAAGC CATCCAAGAA GGACTATCCT 
GATTATTATA AAATCATCTT AGAGCCAATG GACCTGAAAA TAATTGAGCA TAACATCCGA 
AATGACAAAT ATGCAGGTGA AGAAGGAATG ATGGAAGACA TGAAACTCAT GTTCCGCAAT 
GCCAGGCACT ACAATGAGGA GGGCTCCCAG GTATACAATG ATGCCCATAT CCTGGAGAAG 
TTACTCAAAG ATAAAAGGAA AGAGCTGGGC CCTCTGCCTG ATGATGATGA CATGGCTTCT 
CCCAAACTTA AATTGAGTAG GAAGAGTGGT GTTTCTCCTA AGAAATCAAA GTACATGACT 
CCAATGCAGC AGAAACTGAA TGAAGTGTAT GAAGCTGTAA AGAACTATAC TGATAAGAGG 
GGTCGCCGCC TTAGTGCTAT ATTTCTAAGA CTCCCCTCTA GATCAGAGCT GCCTGACTAC 
TACCTGACCA TTAAAAAGCC CATGGACATG GAAAAAATTC GAAGTCACAT GATGGCAAAC 
AAGTACCAAG ACATAGATTC TATGGTAGAG GACTTTGTCA TGATGTTTAA TAATGCCTGT 
ACCTACAATG AACCAGAGTC TTTGATCTAC AAAGATGCCC TTGTACTGCA TAAAGTCCTC 
CTTGAGACTC GGAGAGACCT GGAGGGAGAT GAGGATTCTC ATGTCCCTAA TGTGACGTTG 
CTGATTCAAG AGCTCATCCA TAACCTTTTT GTGTCAGTCA TGAGTCATCA GGATGACGAA 
GGGAGGTGTT ACAGCGACTC CTTAGCAGAA ATTCCTGCTG TGGATCCCAA CTCTCCCAAT 
AAACCTCCCC TTACATTTGA CATTATCAGG AAAAATGTTG AAAGTAATCG GTATCGGCGA 
CTTGATTTAT TTCAGGAGCA TATGTTTGAA GTATTGGAAC GGGCAAGAAG GATGAACCGG 
ACAGATTCCG AAATATATGA GGATGCTGTA GAACTTCAGC AGTTTTTTAT TAGAATTCGT 
GATGAACTCT GCAAAAATGG AGAGATCCTT CTTTCTCCAG CACTCAGCTA TACCACAAAA 
CACTTGCATA ACGATGTGGA AAAAGAAAAA AAGGAAAAAT TGCCTAAAGA AATAGAGGAA 
GATAAACTAA AACGCGAAGA AGAAAAAAGA GAAGCTGAAA AAAGTGAAGA TTCCTCAGGT 
ACTACAGGCC TCTCAGGCTT ACATCGTACA TACAGCCAGG ACTGCAGCTT TAAGAACAGC 
ATGTATCATG TCGGAGATTA TGTCTATGTT GAACCTGCGG AGGCCAATCT ACAACCACAT 
ATAGTGTGTA TTGAGAGACT GTGGGAGGAT TCAGCTGGTG AAAAATGGTT GTACGGCTGT 
TGGTTTTATC GGCCAAATGA AACATTCCAT TTGGCTACAC GAAAATTTCT AGAAAAAGAA 
GTTTTTAAGA GTGACTACTA CAATAAAGTA CCTGTTAGTA AAATTCTAGG CAAATGTGTA 
GTCATGTTTG TCAAGGAATA CTTTAAATTA TGTCCAGAAA ACTTTCGCGA TGAGGATGTT 
TTTGTCTGTG AATCGAGGTA TTCTGCCAAA ACCAAATCTT TTAAGAAAAT TAAACTGTGG 
ACCATGCCCA TCAGTTCAGT TAGATTTGTC CCTCGGGATG TGCCTTTGCC TGTGGTCCGA 
GTGGCCTCTG TGTTTGCAAA TGCAGATAAA GGGGATGATG AGAAGAATAC AGACAACTCA 
GATGACAATA GAGCTGAAGA CAATTTTAAC TTGGAAAAGG AAAAAGAAGA TGTTCCTGTG 
GAGATGTCCA ATGGTGAGCC AGGTTGCCAC TACTTTGAGC AGCTTCGGTA CAATGACATG 
TGGCTGAAGG TTGGTGATTG TGTCTTCATC AAATCCCACG GCTTGGTGCG CCCTCGTGTG 
GGCAGAATTG AGAAAGTATG GGTCCGAGAT GGAGCTGCAT ATTTTTATGG CCCTATCTTC 
ATTCATCCAG AAGAAACAGA ACATGAGCCC ACAAAAATGT TCTACAAAAA AGAAGTGTTT 
CTGAGTAATC TGGAAGAGAC CTGCCCTATG AGTTGTATTC TGGGGAAATG TGCAGTGCTG 
TCATTCAAGG ACTTCCTCTC CTGCAGGCCA ACTGAAATAC CAGAAAATGA CATTCTGCTT 
TGTGAGAGCC GCTATAATGA GAGTGACAAG CAGATGAAGA AGTTCAAGGG TTTGAAGAGG 
TTTTCACTCT CTGCTAAAGT TGTAGATGAT GAAATCTACT ACTTCAGAAA ACCAATCATT 
CCTCAGAAGG AACCCTCACC TTTGTTAGAA AAGAAGATAC AATTGCTAGA AGCTAAATTT 
GCAGAGTTAG AAGGAGGAGA TGATGATATT GAGGAGATGG GAGAAGAGGA TAGTGAAGTC 
ATTGAAGCTC CATCTCTACC TCAACTGCAG ACACCCCTGG CCAATGAGTT GGACCTCATG 
CCCTATACAC CCCCACAGTC TACCCCAAAG TCTGCCAAAG GCAGTGCAAA GAAGGAAAGT 
TCTAAACGAA AAATCAACAT GAGTGGCTAC ATTTTGTTCA GCAGTGAAAT GAGAGCTGTG 
ATTAAAGCCC AGCACCCAGA CTACTCTTTT GGGGAGCTCA GCAGACTGGT GGGGACAGAA 
TGGAGAAACC TTGAAACAGC CAAGAAAGCA GAATATGAAG GTGTGATGAA TCAAGGAGTA 
GCCCCCATGG TAGGGACTCC AGCACCAGGT GGAAGTCCGT ATGGACAACA GGTAGGAGTT 
TTGGGACCTC CAGGGCAGCA GGCACCACCT CCATATCCTG GTCCTCATCC AGCTGGCCCC 
CCTGTCATAC AGCAGCCAAC AACGCCCATG TTTGTGGCTC CCCCACCAAA GACCCAAAGG 
CTTCTCCACT CAGAGGCCTA CCTGAAATAC ATTGAAGGAC TCAGTGCTGA ATCCAACAGC 
ATTAGCAAGT GGGACCAAAC TTTGGCAGCT CGAAGACGGG ATGTCCATTT GTCCAAAGAA 
CAGGAGAGCC GCCTACCTTC TCACTGGCTC AAAAGTAAAG GGGCACACAC CACCATGGCA 
GATGCCCTCT GGCGCCTACG GGATTTAATG CTTCGAGACA CTCTCAACAT CCGACAGGCA 
TACAACCTAG AAAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( NM_001081251.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074720.1 Mus musculus polybromo 1 (Pbrm1), transcript variant 1, mRNA.

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CloneID	OMu01967
Clone ID Related Accession (Same CDS sequence)	NM_001081251.1
Accession Version	NM_001081251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154727.2 and AC154446.2. On Feb 6, 2007 this sequence version replaced XM_619217.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA AGTTCTAAAC GAAAAATCAA CATGAGTGGC 
TACATTTTGT TCAGCAGTGA AATGAGAGCT GTGATTAAAG CCCAGCACCC AGACTACTCT 
TTTGGGGAGC TCAGCAGACT GGTGGGGACA GAATGGAGAA ACCTTGAAAC AGCCAAGAAA 
GCAGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCTCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCAGG CATCCCACCA CCGGGTGTGA TGAATCAAGG AGTAGCCCCC 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCGTATGGAC AACAGGTAGG AGTTTTGGGA 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCTGGTCCTC ATCCAGCTGG CCCCCCTGTC 
ATACAGCAGC CAACAACGCC CATGTTTGTG GCTCCCCCAC CAAAGACCCA AAGGCTTCTC 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CTGAATCCAA CAGCATTAGC 
AAGTGGGACC AAACTTTGGC AGCTCGAAGA CGGGATGTCC ATTTGTCCAA AGAACAGGAG 
AGCCGCCTAC CTTCTCACTG GCTCAAAAGT AAAGGGGCAC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TACGGGATTT AATGCTTCGA GACACTCTCA ACATCCGACA GGCATACAAC 
CTAGAAAATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074720.1
CDS	312..5426
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	NM_001081251.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081251.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA AGTTCTAAAC GAAAAATCAA CATGAGTGGC 
TACATTTTGT TCAGCAGTGA AATGAGAGCT GTGATTAAAG CCCAGCACCC AGACTACTCT 
TTTGGGGAGC TCAGCAGACT GGTGGGGACA GAATGGAGAA ACCTTGAAAC AGCCAAGAAA 
GCAGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCTCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCAGG CATCCCACCA CCGGGTGTGA TGAATCAAGG AGTAGCCCCC 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCGTATGGAC AACAGGTAGG AGTTTTGGGA 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCTGGTCCTC ATCCAGCTGG CCCCCCTGTC 
ATACAGCAGC CAACAACGCC CATGTTTGTG GCTCCCCCAC CAAAGACCCA AAGGCTTCTC 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CTGAATCCAA CAGCATTAGC 
AAGTGGGACC AAACTTTGGC AGCTCGAAGA CGGGATGTCC ATTTGTCCAA AGAACAGGAG 
AGCCGCCTAC CTTCTCACTG GCTCAAAAGT AAAGGGGCAC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TACGGGATTT AATGCTTCGA GACACTCTCA ACATCCGACA GGCATACAAC 
CTAGAAAATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( NM_001359856.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346785.1 Mus musculus polybromo 1 (Pbrm1), transcript variant 4, mRNA.

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CloneID	OMu50273
Clone ID Related Accession (Same CDS sequence)	XM_006519427.3 , NM_001359856.1
Accession Version	NM_001359856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154727.2, AK166588.1, AK165182.1, AI615867.1, CA557251.1, AI507524.1, CD803060.1, BG065752.2, CN707140.1, CN530699.1, CD803036.1, CN537785.1, BQ558912.1, AC154446.2 and AW048644.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.91236.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GGTGTGATGA ATCAAGGAGT AGCCCCCATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAAC AGGTAGGAGT TTTGGGACCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGTCCTCATC CAGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACGCCCAT GTTTGTGGCT CCCCCACCAA AGACCCAAAG GCTTCTCCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCTG AATCCAACAG CATTAGCAAG 
TGGGACCAAA CTTTGGCAGC TCGAAGACGG GATGTCCATT TGTCCAAAGA ACAGGAGAGC 
CGCCTACCTT CTCACTGGCT CAAAAGTAAA GGGGCACACA CCACCATGGC AGATGCCCTC 
TGGCGCCTAC GGGATTTAAT GCTTCGAGAC ACTCTCAACA TCCGACAGGC ATACAACCTA 
GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346785.1
CDS	436..5247
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	NM_001359856.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359856.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GGTGTGATGA ATCAAGGAGT AGCCCCCATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAAC AGGTAGGAGT TTTGGGACCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGTCCTCATC CAGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACGCCCAT GTTTGTGGCT CCCCCACCAA AGACCCAAAG GCTTCTCCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCTG AATCCAACAG CATTAGCAAG 
TGGGACCAAA CTTTGGCAGC TCGAAGACGG GATGTCCATT TGTCCAAAGA ACAGGAGAGC 
CGCCTACCTT CTCACTGGCT CAAAAGTAAA GGGGCACACA CCACCATGGC AGATGCCCTC 
TGGCGCCTAC GGGATTTAAT GCTTCGAGAC ACTCTCAACA TCCGACAGGC ATACAACCTA 
GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519403.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519466.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X3, mRNA.

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CloneID	OMu50253
Clone ID Related Accession (Same CDS sequence)	XM_006519403.1 , XM_006519405.3 , XM_017316137.1 , XM_006519402.3 , XM_017316138.1
Accession Version	XM_006519403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519466.1
CDS	441..5633
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519403.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519403.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519405.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519468.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X5, mRNA.

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CloneID	OMu50253
Clone ID Related Accession (Same CDS sequence)	XM_006519403.1 , XM_006519405.3 , XM_017316137.1 , XM_006519402.3 , XM_017316138.1
Accession Version	XM_006519405.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519405.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519468.1
CDS	190..5382
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519405.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519405.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316137.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171626.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X2, mRNA.

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CloneID	OMu50253
Clone ID Related Accession (Same CDS sequence)	XM_006519403.1 , XM_006519405.3 , XM_017316137.1 , XM_006519402.3 , XM_017316138.1
Accession Version	XM_017316137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171626.1
CDS	443..5635
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316137.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316137.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519402.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519465.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu50253
Clone ID Related Accession (Same CDS sequence)	XM_006519403.1 , XM_006519405.3 , XM_017316137.1 , XM_006519402.3 , XM_017316138.1
Accession Version	XM_006519402.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519402.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519465.1
CDS	359..5551
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519402.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519402.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316138.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171627.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu50253
Clone ID Related Accession (Same CDS sequence)	XM_006519403.1 , XM_006519405.3 , XM_017316137.1 , XM_006519402.3 , XM_017316138.1
Accession Version	XM_017316138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171627.1
CDS	337..5529
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316138.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316138.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316139.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171628.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X7, mRNA.

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CloneID	OMu50254
Clone ID Related Accession (Same CDS sequence)	XM_017316139.1 , XM_006519406.3
Accession Version	XM_017316139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171628.1
CDS	443..5632
Translation	MRRLAFRGAGCALVKLKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316139.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316139.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519406.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519469.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X6, mRNA.

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CloneID	OMu50254
Clone ID Related Accession (Same CDS sequence)	XM_017316139.1 , XM_006519406.3
Accession Version	XM_006519406.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519406.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519469.1
CDS	359..5548
Translation	MRRLAFRGAGCALVKLKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519406.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519406.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519407.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519470.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X8, mRNA.

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CloneID	OMu50255
Clone ID Related Accession (Same CDS sequence)	XM_006519407.3 , XM_017316140.1 , XM_017316141.1
Accession Version	XM_006519407.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519407.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGCTTTC TGTCTTACAG 
TCTACCCCAA AGTCTGCCAA AGGCAGTGCA AAGAAGGAAA GTTCTAAACG AAAAATCAAC 
ATGAGTGGCT ACATTTTGTT CAGCAGTGAA ATGAGAGCTG TGATTAAAGC CCAGCACCCA 
GACTACTCTT TTGGGGAGCT CAGCAGACTG GTGGGGACAG AATGGAGAAA CCTTGAAACA 
GCCAAGAAAG CAGAATATGA AGAGCGGGCA GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG 
CGAGCAGCAC AGCAACAGCA GCCGAGTGCT TCTCCCCGAG CAGGCACCCC TGTGGGGGCT 
CTCATGGGGG TGGTGCCACC ACCAACACCA ATGGGGATGC TCAATCAGCA GTTGACACCT 
GTTGCAGGCA TGATGGGTGG CTATCCGCCA GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT 
GGCCTTGTTA GCATGGGCAG CATGCAGCCA CTTCACCCTG GGGGGCCTCC ACCTCACCAT 
CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC ATCCCACCAC CGGGTGTGAT GAATCAAGGA 
GTAGCCCCCA TGGTAGGGAC TCCAGCACCA GGTGGAAGTC CGTATGGACA ACAGGTAGGA 
GTTTTGGGAC CTCCAGGGCA GCAGGCACCA CCTCCATATC CTGGTCCTCA TCCAGCTGGC 
CCCCCTGTCA TACAGCAGCC AACAACGCCC ATGTTTGTGG CTCCCCCACC AAAGACCCAA 
AGGCTTCTCC ACTCAGAGGC CTACCTGAAA TACATTGAAG GACTCAGTGC TGAATCCAAC 
AGCATTAGCA AGTGGGACCA AACTTTGGCA GCTCGAAGAC GGGATGTCCA TTTGTCCAAA 
GAACAGGAGA GCCGCCTACC TTCTCACTGG CTCAAAAGTA AAGGGGCACA CACCACCATG 
GCAGATGCCC TCTGGCGCCT ACGGGATTTA ATGCTTCGAG ACACTCTCAA CATCCGACAG 
GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519470.1
CDS	359..5542
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519407.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519407.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGCTTTC TGTCTTACAG 
TCTACCCCAA AGTCTGCCAA AGGCAGTGCA AAGAAGGAAA GTTCTAAACG AAAAATCAAC 
ATGAGTGGCT ACATTTTGTT CAGCAGTGAA ATGAGAGCTG TGATTAAAGC CCAGCACCCA 
GACTACTCTT TTGGGGAGCT CAGCAGACTG GTGGGGACAG AATGGAGAAA CCTTGAAACA 
GCCAAGAAAG CAGAATATGA AGAGCGGGCA GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG 
CGAGCAGCAC AGCAACAGCA GCCGAGTGCT TCTCCCCGAG CAGGCACCCC TGTGGGGGCT 
CTCATGGGGG TGGTGCCACC ACCAACACCA ATGGGGATGC TCAATCAGCA GTTGACACCT 
GTTGCAGGCA TGATGGGTGG CTATCCGCCA GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT 
GGCCTTGTTA GCATGGGCAG CATGCAGCCA CTTCACCCTG GGGGGCCTCC ACCTCACCAT 
CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC ATCCCACCAC CGGGTGTGAT GAATCAAGGA 
GTAGCCCCCA TGGTAGGGAC TCCAGCACCA GGTGGAAGTC CGTATGGACA ACAGGTAGGA 
GTTTTGGGAC CTCCAGGGCA GCAGGCACCA CCTCCATATC CTGGTCCTCA TCCAGCTGGC 
CCCCCTGTCA TACAGCAGCC AACAACGCCC ATGTTTGTGG CTCCCCCACC AAAGACCCAA 
AGGCTTCTCC ACTCAGAGGC CTACCTGAAA TACATTGAAG GACTCAGTGC TGAATCCAAC 
AGCATTAGCA AGTGGGACCA AACTTTGGCA GCTCGAAGAC GGGATGTCCA TTTGTCCAAA 
GAACAGGAGA GCCGCCTACC TTCTCACTGG CTCAAAAGTA AAGGGGCACA CACCACCATG 
GCAGATGCCC TCTGGCGCCT ACGGGATTTA ATGCTTCGAG ACACTCTCAA CATCCGACAG 
GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316140.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171629.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X9, mRNA.

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CloneID	OMu50255
Clone ID Related Accession (Same CDS sequence)	XM_006519407.3 , XM_017316140.1 , XM_017316141.1
Accession Version	XM_017316140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGCTTTC TGTCTTACAG 
TCTACCCCAA AGTCTGCCAA AGGCAGTGCA AAGAAGGAAA GTTCTAAACG AAAAATCAAC 
ATGAGTGGCT ACATTTTGTT CAGCAGTGAA ATGAGAGCTG TGATTAAAGC CCAGCACCCA 
GACTACTCTT TTGGGGAGCT CAGCAGACTG GTGGGGACAG AATGGAGAAA CCTTGAAACA 
GCCAAGAAAG CAGAATATGA AGAGCGGGCA GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG 
CGAGCAGCAC AGCAACAGCA GCCGAGTGCT TCTCCCCGAG CAGGCACCCC TGTGGGGGCT 
CTCATGGGGG TGGTGCCACC ACCAACACCA ATGGGGATGC TCAATCAGCA GTTGACACCT 
GTTGCAGGCA TGATGGGTGG CTATCCGCCA GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT 
GGCCTTGTTA GCATGGGCAG CATGCAGCCA CTTCACCCTG GGGGGCCTCC ACCTCACCAT 
CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC ATCCCACCAC CGGGTGTGAT GAATCAAGGA 
GTAGCCCCCA TGGTAGGGAC TCCAGCACCA GGTGGAAGTC CGTATGGACA ACAGGTAGGA 
GTTTTGGGAC CTCCAGGGCA GCAGGCACCA CCTCCATATC CTGGTCCTCA TCCAGCTGGC 
CCCCCTGTCA TACAGCAGCC AACAACGCCC ATGTTTGTGG CTCCCCCACC AAAGACCCAA 
AGGCTTCTCC ACTCAGAGGC CTACCTGAAA TACATTGAAG GACTCAGTGC TGAATCCAAC 
AGCATTAGCA AGTGGGACCA AACTTTGGCA GCTCGAAGAC GGGATGTCCA TTTGTCCAAA 
GAACAGGAGA GCCGCCTACC TTCTCACTGG CTCAAAAGTA AAGGGGCACA CACCACCATG 
GCAGATGCCC TCTGGCGCCT ACGGGATTTA ATGCTTCGAG ACACTCTCAA CATCCGACAG 
GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171629.1
CDS	443..5626
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316140.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316140.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGCTTTC TGTCTTACAG 
TCTACCCCAA AGTCTGCCAA AGGCAGTGCA AAGAAGGAAA GTTCTAAACG AAAAATCAAC 
ATGAGTGGCT ACATTTTGTT CAGCAGTGAA ATGAGAGCTG TGATTAAAGC CCAGCACCCA 
GACTACTCTT TTGGGGAGCT CAGCAGACTG GTGGGGACAG AATGGAGAAA CCTTGAAACA 
GCCAAGAAAG CAGAATATGA AGAGCGGGCA GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG 
CGAGCAGCAC AGCAACAGCA GCCGAGTGCT TCTCCCCGAG CAGGCACCCC TGTGGGGGCT 
CTCATGGGGG TGGTGCCACC ACCAACACCA ATGGGGATGC TCAATCAGCA GTTGACACCT 
GTTGCAGGCA TGATGGGTGG CTATCCGCCA GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT 
GGCCTTGTTA GCATGGGCAG CATGCAGCCA CTTCACCCTG GGGGGCCTCC ACCTCACCAT 
CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC ATCCCACCAC CGGGTGTGAT GAATCAAGGA 
GTAGCCCCCA TGGTAGGGAC TCCAGCACCA GGTGGAAGTC CGTATGGACA ACAGGTAGGA 
GTTTTGGGAC CTCCAGGGCA GCAGGCACCA CCTCCATATC CTGGTCCTCA TCCAGCTGGC 
CCCCCTGTCA TACAGCAGCC AACAACGCCC ATGTTTGTGG CTCCCCCACC AAAGACCCAA 
AGGCTTCTCC ACTCAGAGGC CTACCTGAAA TACATTGAAG GACTCAGTGC TGAATCCAAC 
AGCATTAGCA AGTGGGACCA AACTTTGGCA GCTCGAAGAC GGGATGTCCA TTTGTCCAAA 
GAACAGGAGA GCCGCCTACC TTCTCACTGG CTCAAAAGTA AAGGGGCACA CACCACCATG 
GCAGATGCCC TCTGGCGCCT ACGGGATTTA ATGCTTCGAG ACACTCTCAA CATCCGACAG 
GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316141.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171630.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X10, mRNA.

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CloneID	OMu50255
Clone ID Related Accession (Same CDS sequence)	XM_006519407.3 , XM_017316140.1 , XM_017316141.1
Accession Version	XM_017316141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGCTTTC TGTCTTACAG 
TCTACCCCAA AGTCTGCCAA AGGCAGTGCA AAGAAGGAAA GTTCTAAACG AAAAATCAAC 
ATGAGTGGCT ACATTTTGTT CAGCAGTGAA ATGAGAGCTG TGATTAAAGC CCAGCACCCA 
GACTACTCTT TTGGGGAGCT CAGCAGACTG GTGGGGACAG AATGGAGAAA CCTTGAAACA 
GCCAAGAAAG CAGAATATGA AGAGCGGGCA GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG 
CGAGCAGCAC AGCAACAGCA GCCGAGTGCT TCTCCCCGAG CAGGCACCCC TGTGGGGGCT 
CTCATGGGGG TGGTGCCACC ACCAACACCA ATGGGGATGC TCAATCAGCA GTTGACACCT 
GTTGCAGGCA TGATGGGTGG CTATCCGCCA GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT 
GGCCTTGTTA GCATGGGCAG CATGCAGCCA CTTCACCCTG GGGGGCCTCC ACCTCACCAT 
CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC ATCCCACCAC CGGGTGTGAT GAATCAAGGA 
GTAGCCCCCA TGGTAGGGAC TCCAGCACCA GGTGGAAGTC CGTATGGACA ACAGGTAGGA 
GTTTTGGGAC CTCCAGGGCA GCAGGCACCA CCTCCATATC CTGGTCCTCA TCCAGCTGGC 
CCCCCTGTCA TACAGCAGCC AACAACGCCC ATGTTTGTGG CTCCCCCACC AAAGACCCAA 
AGGCTTCTCC ACTCAGAGGC CTACCTGAAA TACATTGAAG GACTCAGTGC TGAATCCAAC 
AGCATTAGCA AGTGGGACCA AACTTTGGCA GCTCGAAGAC GGGATGTCCA TTTGTCCAAA 
GAACAGGAGA GCCGCCTACC TTCTCACTGG CTCAAAAGTA AAGGGGCACA CACCACCATG 
GCAGATGCCC TCTGGCGCCT ACGGGATTTA ATGCTTCGAG ACACTCTCAA CATCCGACAG 
GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171630.1
CDS	219..5402
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316141.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316141.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCCAC CTCTCCTTCC AGCAGTGTCA GTGGAGACTT TGATGACGGG 
CACCATTCTG TGCCTACACC AGGCCCAAGC AGGAAAAGGA GAAGACTGTC CAATCTTCCA 
ACTGTAGATC CTATTGCTGT GTGCCATGAA CTCTATAACA CCATCCGAGA CTATAAGGAT 
GAACAGGGCA GACTCCTCTG TGAGCTGTTC ATTAGGGCTC CAAAGCGGAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTTAAA 
ATGGAAGAGT ATGATGATGT TAATCTACTG ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ACTATAAGCC AGATTCCCCT GAGTATAAAG CTGCTTGTAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAAGGAG AAGCAGACGA TGAAGATGAT 
GACGAAGATG GGCAAGACAA TCAAGGCACA CTGGCTGACG GCTCTTCTCC AGGTTATCTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGG 
CTCATCAGTG AACTTTTTCA GAAACTGCCT TCCAAAGTGC AATATCCAGA CTATTATGCA 
ATAATTAAGG AACCTATAGA TCTCAAGACC ATTGCTCAGA GGATACAGAA TGGAAGCTAC 
AAAAGTATAC ACGCAATGGC CAAAGATATA GATCTTCTAG CAAAAAATGC CAAAACATAC 
AATGAGCCTG GGTCTCAAGT ATTCAAGGAT GCCAATTCGA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TCATGAAATG ACTAAATCAA GTCTTCGAAT AAGGACTGCA 
TCAAATTTGG CTGCAGCCAG GCTGACAGGT CCTTCGCACA ATAAAAGCAG CCTTGGTGAA 
GAAAGAAACC CCACTAGCAA GTATTACCGT AATAAAAGAG CAGTCCAAGG GGGTCGCTTG 
TCAGCAATTA CCATGGCACT TCAGTATGGA TCAGAGAGTG AAGAGGACGC TGCTTTAGCT 
GCTGCACGCT ATGAAGAAGG GGAATCTGAA GCAGAGAGCA TCACTTCCTT CATGGACGTT 
TCCAACCCCT TTCATCAGCT TTACGACACA GTTAGGAGCT GTAGGAATCA CCAAGGGCAG 
CTCATAGCTG AACCTTTCTT CCATTTGCCT TCAAAGAAAA AATACCCAGA TTATTATCAG 
CAAATTAAAA TGCCCATATC ACTTCAACAG ATCAGAACAA AGCTAAAGAA CCAAGAATAT 
GAAACTTTAG ATCATTTGGA GTGTGATCTG AATTTAATGT TTGAAAATGC CAAACGTTAT 
AACGTTCCCA ATTCAGCCAT CTATAAGCGA GTTCTAAAAC TGCAGCAAGT CATGCAGGCA 
AAGAAGAAGG AGCTTGCGAG GAGAGATGAC ATTGAGGACG GAGACAGCAT GATCTCCTCA 
GCCACTTCTG ACACTGGTAG TGCCAAAAGG AAAAGGAATA CTCATGACAG TGAGATGTTG 
GGTCTCAGGA GGCTATCCAG TAAAAAGAAC ATAAGAAAAC AGCGAATGAA AATTTTATTC 
AATGTTGTTC TTGAAGCTCG AGAGCCAGGT TCAGGCAGAA GACTTTGCGA TCTATTTATG 
GTTAAGCCAT CCAAGAAGGA CTATCCTGAT TATTATAAAA TCATCTTAGA GCCAATGGAC 
CTGAAAATAA TTGAGCATAA CATCCGAAAT GACAAATATG CAGGTGAAGA AGGAATGATG 
GAAGACATGA AACTCATGTT CCGCAATGCC AGGCACTACA ATGAGGAGGG CTCCCAGGTA 
TACAATGATG CCCATATCCT GGAGAAGTTA CTCAAAGATA AAAGGAAAGA GCTGGGCCCT 
CTGCCTGATG ATGATGACAT GGCTTCTCCC AAACTTAAAT TGAGTAGGAA GAGTGGTGTT 
TCTCCTAAGA AATCAAAGTA CATGACTCCA ATGCAGCAGA AACTGAATGA AGTGTATGAA 
GCTGTAAAGA ACTATACTGA TAAGAGGGGT CGCCGCCTTA GTGCTATATT TCTAAGACTC 
CCCTCTAGAT CAGAGCTGCC TGACTACTAC CTGACCATTA AAAAGCCCAT GGACATGGAA 
AAAATTCGAA GTCACATGAT GGCAAACAAG TACCAAGACA TAGATTCTAT GGTAGAGGAC 
TTTGTCATGA TGTTTAATAA TGCCTGTACC TACAATGAAC CAGAGTCTTT GATCTACAAA 
GATGCCCTTG TACTGCATAA AGTCCTCCTT GAGACTCGGA GAGACCTGGA GGGAGATGAG 
GATTCTCATG TCCCTAATGT GACGTTGCTG ATTCAAGAGC TCATCCATAA CCTTTTTGTG 
TCAGTCATGA GTCATCAGGA TGACGAAGGG AGGTGTTACA GCGACTCCTT AGCAGAAATT 
CCTGCTGTGG ATCCCAACTC TCCCAATAAA CCTCCCCTTA CATTTGACAT TATCAGGAAA 
AATGTTGAAA GTAATCGGTA TCGGCGACTT GATTTATTTC AGGAGCATAT GTTTGAAGTA 
TTGGAACGGG CAAGAAGGAT GAACCGGACA GATTCCGAAA TATATGAGGA TGCTGTAGAA 
CTTCAGCAGT TTTTTATTAG AATTCGTGAT GAACTCTGCA AAAATGGAGA GATCCTTCTT 
TCTCCAGCAC TCAGCTATAC CACAAAACAC TTGCATAACG ATGTGGAAAA AGAAAAAAAG 
GAAAAATTGC CTAAAGAAAT AGAGGAAGAT AAACTAAAAC GCGAAGAAGA AAAAAGAGAA 
GCTGAAAAAA GTGAAGATTC CTCAGGTACT ACAGGCCTCT CAGGCTTACA TCGTACATAC 
AGCCAGGACT GCAGCTTTAA GAACAGCATG TATCATGTCG GAGATTATGT CTATGTTGAA 
CCTGCGGAGG CCAATCTACA ACCACATATA GTGTGTATTG AGAGACTGTG GGAGGATTCA 
GCTGGTGAAA AATGGTTGTA CGGCTGTTGG TTTTATCGGC CAAATGAAAC ATTCCATTTG 
GCTACACGAA AATTTCTAGA AAAAGAAGTT TTTAAGAGTG ACTACTACAA TAAAGTACCT 
GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC ATGTTTGTCA AGGAATACTT TAAATTATGT 
CCAGAAAACT TTCGCGATGA GGATGTTTTT GTCTGTGAAT CGAGGTATTC TGCCAAAACC 
AAATCTTTTA AGAAAATTAA ACTGTGGACC ATGCCCATCA GTTCAGTTAG ATTTGTCCCT 
CGGGATGTGC CTTTGCCTGT GGTCCGAGTG GCCTCTGTGT TTGCAAATGC AGATAAAGGG 
GATGATGAGA AGAATACAGA CAACTCAGAT GACAATAGAG CTGAAGACAA TTTTAACTTG 
GAAAAGGAAA AAGAAGATGT TCCTGTGGAG ATGTCCAATG GTGAGCCAGG TTGCCACTAC 
TTTGAGCAGC TTCGGTACAA TGACATGTGG CTGAAGGTTG GTGATTGTGT CTTCATCAAA 
TCCCACGGCT TGGTGCGCCC TCGTGTGGGC AGAATTGAGA AAGTATGGGT CCGAGATGGA 
GCTGCATATT TTTATGGCCC TATCTTCATT CATCCAGAAG AAACAGAACA TGAGCCCACA 
AAAATGTTCT ACAAAAAAGA AGTGTTTCTG AGTAATCTGG AAGAGACCTG CCCTATGAGT 
TGTATTCTGG GGAAATGTGC AGTGCTGTCA TTCAAGGACT TCCTCTCCTG CAGGCCAACT 
GAAATACCAG AAAATGACAT TCTGCTTTGT GAGAGCCGCT ATAATGAGAG TGACAAGCAG 
ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT TCACTCTCTG CTAAAGTTGT AGATGATGAA 
ATCTACTACT TCAGAAAACC AATCATTCCT CAGAAGGAAC CCTCACCTTT GTTAGAAAAG 
AAGATACAAT TGCTAGAAGC TAAATTTGCA GAGTTAGAAG GAGGAGATGA TGATATTGAG 
GAGATGGGAG AAGAGGATAG TGAAGTCATT GAAGCTCCAT CTCTACCTCA ACTGCAGACA 
CCCCTGGCCA ATGAGTTGGA CCTCATGCCC TATACACCCC CACAGCTTTC TGTCTTACAG 
TCTACCCCAA AGTCTGCCAA AGGCAGTGCA AAGAAGGAAA GTTCTAAACG AAAAATCAAC 
ATGAGTGGCT ACATTTTGTT CAGCAGTGAA ATGAGAGCTG TGATTAAAGC CCAGCACCCA 
GACTACTCTT TTGGGGAGCT CAGCAGACTG GTGGGGACAG AATGGAGAAA CCTTGAAACA 
GCCAAGAAAG CAGAATATGA AGAGCGGGCA GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG 
CGAGCAGCAC AGCAACAGCA GCCGAGTGCT TCTCCCCGAG CAGGCACCCC TGTGGGGGCT 
CTCATGGGGG TGGTGCCACC ACCAACACCA ATGGGGATGC TCAATCAGCA GTTGACACCT 
GTTGCAGGCA TGATGGGTGG CTATCCGCCA GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT 
GGCCTTGTTA GCATGGGCAG CATGCAGCCA CTTCACCCTG GGGGGCCTCC ACCTCACCAT 
CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC ATCCCACCAC CGGGTGTGAT GAATCAAGGA 
GTAGCCCCCA TGGTAGGGAC TCCAGCACCA GGTGGAAGTC CGTATGGACA ACAGGTAGGA 
GTTTTGGGAC CTCCAGGGCA GCAGGCACCA CCTCCATATC CTGGTCCTCA TCCAGCTGGC 
CCCCCTGTCA TACAGCAGCC AACAACGCCC ATGTTTGTGG CTCCCCCACC AAAGACCCAA 
AGGCTTCTCC ACTCAGAGGC CTACCTGAAA TACATTGAAG GACTCAGTGC TGAATCCAAC 
AGCATTAGCA AGTGGGACCA AACTTTGGCA GCTCGAAGAC GGGATGTCCA TTTGTCCAAA 
GAACAGGAGA GCCGCCTACC TTCTCACTGG CTCAAAAGTA AAGGGGCACA CACCACCATG 
GCAGATGCCC TCTGGCGCCT ACGGGATTTA ATGCTTCGAG ACACTCTCAA CATCCGACAG 
GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519408.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519471.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu50256
Clone ID Related Accession (Same CDS sequence)	XM_006519408.3 , XM_017316142.1
Accession Version	XM_006519408.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519408.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGTTGGATTC CATGGGTTCC 
AAGAGAAGAA GAGCCACCTC TCCTTCCAGC AGTGTCAGTG GAGACTTTGA TGACGGGCAC 
CATTCTGTGC CTACACCAGG CCCAAGCAGG AAAAGGAGAA GACTGTCCAA TCTTCCAACT 
GTAGATCCTA TTGCTGTGTG CCATGAACTC TATAACACCA TCCGAGACTA TAAGGATGAA 
CAGGGCAGAC TCCTCTGTGA GCTGTTCATT AGGGCTCCAA AGCGGAGAAA TCAACCAGAC 
TATTATGAAG TGGTTTCTCA GCCCATTGAC TTGATGAAAA TCCAACAGAA ACTTAAAATG 
GAAGAGTATG ATGATGTTAA TCTACTGACT GCTGACTTCC AGCTGCTTTT TAACAATGCA 
AAGGCCTACT ATAAGCCAGA TTCCCCTGAG TATAAAGCTG CTTGTAAACT CTGGGATTTG 
TACCTTCGAA CAAGAAATGA GTTTGTTCAG AAAGGAGAAG CAGACGATGA AGATGATGAC 
GAAGATGGGC AAGACAATCA AGGCACACTG GCTGACGGCT CTTCTCCAGG TTATCTGAAG 
GAGATCCTGG AGCAGCTTCT TGAAGCCATA GTTGTAGCCA CAAATCCATC AGGACGGCTC 
ATCAGTGAAC TTTTTCAGAA ACTGCCTTCC AAAGTGCAAT ATCCAGACTA TTATGCAATA 
ATTAAGGAAC CTATAGATCT CAAGACCATT GCTCAGAGGA TACAGAATGG AAGCTACAAA 
AGTATACACG CAATGGCCAA AGATATAGAT CTTCTAGCAA AAAATGCCAA AACATACAAT 
GAGCCTGGGT CTCAAGTATT CAAGGATGCC AATTCGATTA AAAAAATATT TTATATGAAA 
AAGGCAGAAA TTGAACATCA TGAAATGACT AAATCAAGTC TTCGAATAAG GACTGCATCA 
AATTTGGCTG CAGCCAGGCT GACAGGTCCT TCGCACAATA AAAGCAGCCT TGGTGAAGAA 
AGAAACCCCA CTAGCAAGTA TTACCGTAAT AAAAGAGCAG TCCAAGGGGG TCGCTTGTCA 
GCAATTACCA TGGCACTTCA GTATGGATCA GAGAGTGAAG AGGACGCTGC TTTAGCTGCT 
GCACGCTATG AAGAAGGGGA ATCTGAAGCA GAGAGCATCA CTTCCTTCAT GGACGTTTCC 
AACCCCTTTC ATCAGCTTTA CGACACAGTT AGGAGCTGTA GGAATCACCA AGGGCAGCTC 
ATAGCTGAAC CTTTCTTCCA TTTGCCTTCA AAGAAAAAAT ACCCAGATTA TTATCAGCAA 
ATTAAAATGC CCATATCACT TCAACAGATC AGAACAAAGC TAAAGAACCA AGAATATGAA 
ACTTTAGATC ATTTGGAGTG TGATCTGAAT TTAATGTTTG AAAATGCCAA ACGTTATAAC 
GTTCCCAATT CAGCCATCTA TAAGCGAGTT CTAAAACTGC AGCAAGTCAT GCAGGCAAAG 
AAGAAGGAGC TTGCGAGGAG AGATGACATT GAGGACGGAG ACAGCATGAT CTCCTCAGCC 
ACTTCTGACA CTGGTAGTGC CAAAAGGAAA AGGAATACTC ATGACAGTGA GATGTTGGGT 
CTCAGGAGGC TATCCAGTAA AAAGAACATA AGAAAACAGC GAATGAAAAT TTTATTCAAT 
GTTGTTCTTG AAGCTCGAGA GCCAGGTTCA GGCAGAAGAC TTTGCGATCT ATTTATGGTT 
AAGCCATCCA AGAAGGACTA TCCTGATTAT TATAAAATCA TCTTAGAGCC AATGGACCTG 
AAAATAATTG AGCATAACAT CCGAAATGAC AAATATGCAG GTGAAGAAGG AATGATGGAA 
GACATGAAAC TCATGTTCCG CAATGCCAGG CACTACAATG AGGAGGGCTC CCAGGTATAC 
AATGATGCCC ATATCCTGGA GAAGTTACTC AAAGATAAAA GGAAAGAGCT GGGCCCTCTG 
CCTGATGATG ATGACATGGC TTCTCCCAAA CTTAAATTGA GTAGGAAGAG TGGTGTTTCT 
CCTAAGAAAT CAAAGTACAT GACTCCAATG CAGCAGAAAC TGAATGAAGT GTATGAAGCT 
GTAAAGAACT ATACTGATAA GAGGGGTCGC CGCCTTAGTG CTATATTTCT AAGACTCCCC 
TCTAGATCAG AGCTGCCTGA CTACTACCTG ACCATTAAAA AGCCCATGGA CATGGAAAAA 
ATTCGAAGTC ACATGATGGC AAACAAGTAC CAAGACATAG ATTCTATGGT AGAGGACTTT 
GTCATGATGT TTAATAATGC CTGTACCTAC AATGAACCAG AGTCTTTGAT CTACAAAGAT 
GCCCTTGTAC TGCATAAAGT CCTCCTTGAG ACTCGGAGAG ACCTGGAGGG AGATGAGGAT 
TCTCATGTCC CTAATGTGAC GTTGCTGATT CAAGAGCTCA TCCATAACCT TTTTGTGTCA 
GTCATGAGTC ATCAGGATGA CGAAGGGAGG TGTTACAGCG ACTCCTTAGC AGAAATTCCT 
GCTGTGGATC CCAACTCTCC CAATAAACCT CCCCTTACAT TTGACATTAT CAGGAAAAAT 
GTTGAAAGTA ATCGGTATCG GCGACTTGAT TTATTTCAGG AGCATATGTT TGAAGTATTG 
GAACGGGCAA GAAGGATGAA CCGGACAGAT TCCGAAATAT ATGAGGATGC TGTAGAACTT 
CAGCAGTTTT TTATTAGAAT TCGTGATGAA CTCTGCAAAA ATGGAGAGAT CCTTCTTTCT 
CCAGCACTCA GCTATACCAC AAAACACTTG CATAACGATG TGGAAAAAGA AAAAAAGGAA 
AAATTGCCTA AAGAAATAGA GGAAGATAAA CTAAAACGCG AAGAAGAAAA AAGAGAAGCT 
GAAAAAAGTG AAGATTCCTC AGGTACTACA GGCCTCTCAG GCTTACATCG TACATACAGC 
CAGGACTGCA GCTTTAAGAA CAGCATGTAT CATGTCGGAG ATTATGTCTA TGTTGAACCT 
GCGGAGGCCA ATCTACAACC ACATATAGTG TGTATTGAGA GACTGTGGGA GGATTCAGCT 
GGTGAAAAAT GGTTGTACGG CTGTTGGTTT TATCGGCCAA ATGAAACATT CCATTTGGCT 
ACACGAAAAT TTCTAGAAAA AGAAGTTTTT AAGAGTGACT ACTACAATAA AGTACCTGTT 
AGTAAAATTC TAGGCAAATG TGTAGTCATG TTTGTCAAGG AATACTTTAA ATTATGTCCA 
GAAAACTTTC GCGATGAGGA TGTTTTTGTC TGTGAATCGA GGTATTCTGC CAAAACCAAA 
TCTTTTAAGA AAATTAAACT GTGGACCATG CCCATCAGTT CAGTTAGATT TGTCCCTCGG 
GATGTGCCTT TGCCTGTGGT CCGAGTGGCC TCTGTGTTTG CAAATGCAGA TAAAGGGGAT 
GATGAGAAGA ATACAGACAA CTCAGATGAC AATAGAGCTG AAGACAATTT TAACTTGGAA 
AAGGAAAAAG AAGATGTTCC TGTGGAGATG TCCAATGGTG AGCCAGGTTG CCACTACTTT 
GAGCAGCTTC GGTACAATGA CATGTGGCTG AAGGTTGGTG ATTGTGTCTT CATCAAATCC 
CACGGCTTGG TGCGCCCTCG TGTGGGCAGA ATTGAGAAAG TATGGGTCCG AGATGGAGCT 
GCATATTTTT ATGGCCCTAT CTTCATTCAT CCAGAAGAAA CAGAACATGA GCCCACAAAA 
ATGTTCTACA AAAAAGAAGT GTTTCTGAGT AATCTGGAAG AGACCTGCCC TATGAGTTGT 
ATTCTGGGGA AATGTGCAGT GCTGTCATTC AAGGACTTCC TCTCCTGCAG GCCAACTGAA 
ATACCAGAAA ATGACATTCT GCTTTGTGAG AGCCGCTATA ATGAGAGTGA CAAGCAGATG 
AAGAAGTTCA AGGGTTTGAA GAGGTTTTCA CTCTCTGCTA AAGTTGTAGA TGATGAAATC 
TACTACTTCA GAAAACCAAT CATTCCTCAG AAGGAACCCT CACCTTTGTT AGAAAAGAAG 
ATACAATTGC TAGAAGCTAA ATTTGCAGAG TTAGAAGGAG GAGATGATGA TATTGAGGAG 
ATGGGAGAAG AGGATAGTGA AGTCATTGAA GCTCCATCTC TACCTCAACT GCAGACACCC 
CTGGCCAATG AGTTGGACCT CATGCCCTAT ACACCCCCAC AGCTTTCTGT CTTACAGTCT 
ACCCCAAAGT CTGCCAAAGG CAGTGCAAAG AAGGAAAGTT CTAAACGAAA AATCAACATG 
AGTGGCTACA TTTTGTTCAG CAGTGAAATG AGAGCTGTGA TTAAAGCCCA GCACCCAGAC 
TACTCTTTTG GGGAGCTCAG CAGACTGGTG GGGACAGAAT GGAGAAACCT TGAAACAGCC 
AAGAAAGCAG AATATGAAGA GCGGGCAGCT AAAGTTGCTG AGCAGCAGGA GAGAGAGCGA 
GCAGCACAGC AACAGCAGCC GAGTGCTTCT CCCCGAGCAG GCACCCCTGT GGGGGCTCTC 
ATGGGGGTGG TGCCACCACC AACACCAATG GGGATGCTCA ATCAGCAGTT GACACCTGTT 
GCAGGCATGA TGGGTGGCTA TCCGCCAGGC CTTCCACCTT TGCAGGGCCC AGTTGATGGC 
CTTGTTAGCA TGGGCAGCAT GCAGCCACTT CACCCTGGGG GGCCTCCACC TCACCATCTT 
CCGCCAGGTG TGCCTGGCCT CCCAGGCATC CCACCACCGG GTGTGATGAA TCAAGGAGTA 
GCCCCCATGG TAGGGACTCC AGCACCAGGT GGAAGTCCGT ATGGACAACA GGTAGGAGTT 
TTGGGACCTC CAGGGCAGCA GGCACCACCT CCATATCCTG GTCCTCATCC AGCTGGCCCC 
CCTGTCATAC AGCAGCCAAC AACGCCCATG TTTGTGGCTC CCCCACCAAA GACCCAAAGG 
CTTCTCCACT CAGAGGCCTA CCTGAAATAC ATTGAAGGAC TCAGTGCTGA ATCCAACAGC 
ATTAGCAAGT GGGACCAAAC TTTGGCAGCT CGAAGACGGG ATGTCCATTT GTCCAAAGAA 
CAGGAGAGCC GCCTACCTTC TCACTGGCTC AAAAGTAAAG GGGCACACAC CACCATGGCA 
GATGCCCTCT GGCGCCTACG GGATTTAATG CTTCGAGACA CTCTCAACAT CCGACAGGCA 
TACAACCTAG AAAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519471.1
CDS	359..5539
Translation	MRRLAFRGAGCALKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519408.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519408.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGTTGGATTC CATGGGTTCC 
AAGAGAAGAA GAGCCACCTC TCCTTCCAGC AGTGTCAGTG GAGACTTTGA TGACGGGCAC 
CATTCTGTGC CTACACCAGG CCCAAGCAGG AAAAGGAGAA GACTGTCCAA TCTTCCAACT 
GTAGATCCTA TTGCTGTGTG CCATGAACTC TATAACACCA TCCGAGACTA TAAGGATGAA 
CAGGGCAGAC TCCTCTGTGA GCTGTTCATT AGGGCTCCAA AGCGGAGAAA TCAACCAGAC 
TATTATGAAG TGGTTTCTCA GCCCATTGAC TTGATGAAAA TCCAACAGAA ACTTAAAATG 
GAAGAGTATG ATGATGTTAA TCTACTGACT GCTGACTTCC AGCTGCTTTT TAACAATGCA 
AAGGCCTACT ATAAGCCAGA TTCCCCTGAG TATAAAGCTG CTTGTAAACT CTGGGATTTG 
TACCTTCGAA CAAGAAATGA GTTTGTTCAG AAAGGAGAAG CAGACGATGA AGATGATGAC 
GAAGATGGGC AAGACAATCA AGGCACACTG GCTGACGGCT CTTCTCCAGG TTATCTGAAG 
GAGATCCTGG AGCAGCTTCT TGAAGCCATA GTTGTAGCCA CAAATCCATC AGGACGGCTC 
ATCAGTGAAC TTTTTCAGAA ACTGCCTTCC AAAGTGCAAT ATCCAGACTA TTATGCAATA 
ATTAAGGAAC CTATAGATCT CAAGACCATT GCTCAGAGGA TACAGAATGG AAGCTACAAA 
AGTATACACG CAATGGCCAA AGATATAGAT CTTCTAGCAA AAAATGCCAA AACATACAAT 
GAGCCTGGGT CTCAAGTATT CAAGGATGCC AATTCGATTA AAAAAATATT TTATATGAAA 
AAGGCAGAAA TTGAACATCA TGAAATGACT AAATCAAGTC TTCGAATAAG GACTGCATCA 
AATTTGGCTG CAGCCAGGCT GACAGGTCCT TCGCACAATA AAAGCAGCCT TGGTGAAGAA 
AGAAACCCCA CTAGCAAGTA TTACCGTAAT AAAAGAGCAG TCCAAGGGGG TCGCTTGTCA 
GCAATTACCA TGGCACTTCA GTATGGATCA GAGAGTGAAG AGGACGCTGC TTTAGCTGCT 
GCACGCTATG AAGAAGGGGA ATCTGAAGCA GAGAGCATCA CTTCCTTCAT GGACGTTTCC 
AACCCCTTTC ATCAGCTTTA CGACACAGTT AGGAGCTGTA GGAATCACCA AGGGCAGCTC 
ATAGCTGAAC CTTTCTTCCA TTTGCCTTCA AAGAAAAAAT ACCCAGATTA TTATCAGCAA 
ATTAAAATGC CCATATCACT TCAACAGATC AGAACAAAGC TAAAGAACCA AGAATATGAA 
ACTTTAGATC ATTTGGAGTG TGATCTGAAT TTAATGTTTG AAAATGCCAA ACGTTATAAC 
GTTCCCAATT CAGCCATCTA TAAGCGAGTT CTAAAACTGC AGCAAGTCAT GCAGGCAAAG 
AAGAAGGAGC TTGCGAGGAG AGATGACATT GAGGACGGAG ACAGCATGAT CTCCTCAGCC 
ACTTCTGACA CTGGTAGTGC CAAAAGGAAA AGGAATACTC ATGACAGTGA GATGTTGGGT 
CTCAGGAGGC TATCCAGTAA AAAGAACATA AGAAAACAGC GAATGAAAAT TTTATTCAAT 
GTTGTTCTTG AAGCTCGAGA GCCAGGTTCA GGCAGAAGAC TTTGCGATCT ATTTATGGTT 
AAGCCATCCA AGAAGGACTA TCCTGATTAT TATAAAATCA TCTTAGAGCC AATGGACCTG 
AAAATAATTG AGCATAACAT CCGAAATGAC AAATATGCAG GTGAAGAAGG AATGATGGAA 
GACATGAAAC TCATGTTCCG CAATGCCAGG CACTACAATG AGGAGGGCTC CCAGGTATAC 
AATGATGCCC ATATCCTGGA GAAGTTACTC AAAGATAAAA GGAAAGAGCT GGGCCCTCTG 
CCTGATGATG ATGACATGGC TTCTCCCAAA CTTAAATTGA GTAGGAAGAG TGGTGTTTCT 
CCTAAGAAAT CAAAGTACAT GACTCCAATG CAGCAGAAAC TGAATGAAGT GTATGAAGCT 
GTAAAGAACT ATACTGATAA GAGGGGTCGC CGCCTTAGTG CTATATTTCT AAGACTCCCC 
TCTAGATCAG AGCTGCCTGA CTACTACCTG ACCATTAAAA AGCCCATGGA CATGGAAAAA 
ATTCGAAGTC ACATGATGGC AAACAAGTAC CAAGACATAG ATTCTATGGT AGAGGACTTT 
GTCATGATGT TTAATAATGC CTGTACCTAC AATGAACCAG AGTCTTTGAT CTACAAAGAT 
GCCCTTGTAC TGCATAAAGT CCTCCTTGAG ACTCGGAGAG ACCTGGAGGG AGATGAGGAT 
TCTCATGTCC CTAATGTGAC GTTGCTGATT CAAGAGCTCA TCCATAACCT TTTTGTGTCA 
GTCATGAGTC ATCAGGATGA CGAAGGGAGG TGTTACAGCG ACTCCTTAGC AGAAATTCCT 
GCTGTGGATC CCAACTCTCC CAATAAACCT CCCCTTACAT TTGACATTAT CAGGAAAAAT 
GTTGAAAGTA ATCGGTATCG GCGACTTGAT TTATTTCAGG AGCATATGTT TGAAGTATTG 
GAACGGGCAA GAAGGATGAA CCGGACAGAT TCCGAAATAT ATGAGGATGC TGTAGAACTT 
CAGCAGTTTT TTATTAGAAT TCGTGATGAA CTCTGCAAAA ATGGAGAGAT CCTTCTTTCT 
CCAGCACTCA GCTATACCAC AAAACACTTG CATAACGATG TGGAAAAAGA AAAAAAGGAA 
AAATTGCCTA AAGAAATAGA GGAAGATAAA CTAAAACGCG AAGAAGAAAA AAGAGAAGCT 
GAAAAAAGTG AAGATTCCTC AGGTACTACA GGCCTCTCAG GCTTACATCG TACATACAGC 
CAGGACTGCA GCTTTAAGAA CAGCATGTAT CATGTCGGAG ATTATGTCTA TGTTGAACCT 
GCGGAGGCCA ATCTACAACC ACATATAGTG TGTATTGAGA GACTGTGGGA GGATTCAGCT 
GGTGAAAAAT GGTTGTACGG CTGTTGGTTT TATCGGCCAA ATGAAACATT CCATTTGGCT 
ACACGAAAAT TTCTAGAAAA AGAAGTTTTT AAGAGTGACT ACTACAATAA AGTACCTGTT 
AGTAAAATTC TAGGCAAATG TGTAGTCATG TTTGTCAAGG AATACTTTAA ATTATGTCCA 
GAAAACTTTC GCGATGAGGA TGTTTTTGTC TGTGAATCGA GGTATTCTGC CAAAACCAAA 
TCTTTTAAGA AAATTAAACT GTGGACCATG CCCATCAGTT CAGTTAGATT TGTCCCTCGG 
GATGTGCCTT TGCCTGTGGT CCGAGTGGCC TCTGTGTTTG CAAATGCAGA TAAAGGGGAT 
GATGAGAAGA ATACAGACAA CTCAGATGAC AATAGAGCTG AAGACAATTT TAACTTGGAA 
AAGGAAAAAG AAGATGTTCC TGTGGAGATG TCCAATGGTG AGCCAGGTTG CCACTACTTT 
GAGCAGCTTC GGTACAATGA CATGTGGCTG AAGGTTGGTG ATTGTGTCTT CATCAAATCC 
CACGGCTTGG TGCGCCCTCG TGTGGGCAGA ATTGAGAAAG TATGGGTCCG AGATGGAGCT 
GCATATTTTT ATGGCCCTAT CTTCATTCAT CCAGAAGAAA CAGAACATGA GCCCACAAAA 
ATGTTCTACA AAAAAGAAGT GTTTCTGAGT AATCTGGAAG AGACCTGCCC TATGAGTTGT 
ATTCTGGGGA AATGTGCAGT GCTGTCATTC AAGGACTTCC TCTCCTGCAG GCCAACTGAA 
ATACCAGAAA ATGACATTCT GCTTTGTGAG AGCCGCTATA ATGAGAGTGA CAAGCAGATG 
AAGAAGTTCA AGGGTTTGAA GAGGTTTTCA CTCTCTGCTA AAGTTGTAGA TGATGAAATC 
TACTACTTCA GAAAACCAAT CATTCCTCAG AAGGAACCCT CACCTTTGTT AGAAAAGAAG 
ATACAATTGC TAGAAGCTAA ATTTGCAGAG TTAGAAGGAG GAGATGATGA TATTGAGGAG 
ATGGGAGAAG AGGATAGTGA AGTCATTGAA GCTCCATCTC TACCTCAACT GCAGACACCC 
CTGGCCAATG AGTTGGACCT CATGCCCTAT ACACCCCCAC AGCTTTCTGT CTTACAGTCT 
ACCCCAAAGT CTGCCAAAGG CAGTGCAAAG AAGGAAAGTT CTAAACGAAA AATCAACATG 
AGTGGCTACA TTTTGTTCAG CAGTGAAATG AGAGCTGTGA TTAAAGCCCA GCACCCAGAC 
TACTCTTTTG GGGAGCTCAG CAGACTGGTG GGGACAGAAT GGAGAAACCT TGAAACAGCC 
AAGAAAGCAG AATATGAAGA GCGGGCAGCT AAAGTTGCTG AGCAGCAGGA GAGAGAGCGA 
GCAGCACAGC AACAGCAGCC GAGTGCTTCT CCCCGAGCAG GCACCCCTGT GGGGGCTCTC 
ATGGGGGTGG TGCCACCACC AACACCAATG GGGATGCTCA ATCAGCAGTT GACACCTGTT 
GCAGGCATGA TGGGTGGCTA TCCGCCAGGC CTTCCACCTT TGCAGGGCCC AGTTGATGGC 
CTTGTTAGCA TGGGCAGCAT GCAGCCACTT CACCCTGGGG GGCCTCCACC TCACCATCTT 
CCGCCAGGTG TGCCTGGCCT CCCAGGCATC CCACCACCGG GTGTGATGAA TCAAGGAGTA 
GCCCCCATGG TAGGGACTCC AGCACCAGGT GGAAGTCCGT ATGGACAACA GGTAGGAGTT 
TTGGGACCTC CAGGGCAGCA GGCACCACCT CCATATCCTG GTCCTCATCC AGCTGGCCCC 
CCTGTCATAC AGCAGCCAAC AACGCCCATG TTTGTGGCTC CCCCACCAAA GACCCAAAGG 
CTTCTCCACT CAGAGGCCTA CCTGAAATAC ATTGAAGGAC TCAGTGCTGA ATCCAACAGC 
ATTAGCAAGT GGGACCAAAC TTTGGCAGCT CGAAGACGGG ATGTCCATTT GTCCAAAGAA 
CAGGAGAGCC GCCTACCTTC TCACTGGCTC AAAAGTAAAG GGGCACACAC CACCATGGCA 
GATGCCCTCT GGCGCCTACG GGATTTAATG CTTCGAGACA CTCTCAACAT CCGACAGGCA 
TACAACCTAG AAAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316142.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171631.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu50256
Clone ID Related Accession (Same CDS sequence)	XM_006519408.3 , XM_017316142.1
Accession Version	XM_017316142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGTTGGATTC CATGGGTTCC 
AAGAGAAGAA GAGCCACCTC TCCTTCCAGC AGTGTCAGTG GAGACTTTGA TGACGGGCAC 
CATTCTGTGC CTACACCAGG CCCAAGCAGG AAAAGGAGAA GACTGTCCAA TCTTCCAACT 
GTAGATCCTA TTGCTGTGTG CCATGAACTC TATAACACCA TCCGAGACTA TAAGGATGAA 
CAGGGCAGAC TCCTCTGTGA GCTGTTCATT AGGGCTCCAA AGCGGAGAAA TCAACCAGAC 
TATTATGAAG TGGTTTCTCA GCCCATTGAC TTGATGAAAA TCCAACAGAA ACTTAAAATG 
GAAGAGTATG ATGATGTTAA TCTACTGACT GCTGACTTCC AGCTGCTTTT TAACAATGCA 
AAGGCCTACT ATAAGCCAGA TTCCCCTGAG TATAAAGCTG CTTGTAAACT CTGGGATTTG 
TACCTTCGAA CAAGAAATGA GTTTGTTCAG AAAGGAGAAG CAGACGATGA AGATGATGAC 
GAAGATGGGC AAGACAATCA AGGCACACTG GCTGACGGCT CTTCTCCAGG TTATCTGAAG 
GAGATCCTGG AGCAGCTTCT TGAAGCCATA GTTGTAGCCA CAAATCCATC AGGACGGCTC 
ATCAGTGAAC TTTTTCAGAA ACTGCCTTCC AAAGTGCAAT ATCCAGACTA TTATGCAATA 
ATTAAGGAAC CTATAGATCT CAAGACCATT GCTCAGAGGA TACAGAATGG AAGCTACAAA 
AGTATACACG CAATGGCCAA AGATATAGAT CTTCTAGCAA AAAATGCCAA AACATACAAT 
GAGCCTGGGT CTCAAGTATT CAAGGATGCC AATTCGATTA AAAAAATATT TTATATGAAA 
AAGGCAGAAA TTGAACATCA TGAAATGACT AAATCAAGTC TTCGAATAAG GACTGCATCA 
AATTTGGCTG CAGCCAGGCT GACAGGTCCT TCGCACAATA AAAGCAGCCT TGGTGAAGAA 
AGAAACCCCA CTAGCAAGTA TTACCGTAAT AAAAGAGCAG TCCAAGGGGG TCGCTTGTCA 
GCAATTACCA TGGCACTTCA GTATGGATCA GAGAGTGAAG AGGACGCTGC TTTAGCTGCT 
GCACGCTATG AAGAAGGGGA ATCTGAAGCA GAGAGCATCA CTTCCTTCAT GGACGTTTCC 
AACCCCTTTC ATCAGCTTTA CGACACAGTT AGGAGCTGTA GGAATCACCA AGGGCAGCTC 
ATAGCTGAAC CTTTCTTCCA TTTGCCTTCA AAGAAAAAAT ACCCAGATTA TTATCAGCAA 
ATTAAAATGC CCATATCACT TCAACAGATC AGAACAAAGC TAAAGAACCA AGAATATGAA 
ACTTTAGATC ATTTGGAGTG TGATCTGAAT TTAATGTTTG AAAATGCCAA ACGTTATAAC 
GTTCCCAATT CAGCCATCTA TAAGCGAGTT CTAAAACTGC AGCAAGTCAT GCAGGCAAAG 
AAGAAGGAGC TTGCGAGGAG AGATGACATT GAGGACGGAG ACAGCATGAT CTCCTCAGCC 
ACTTCTGACA CTGGTAGTGC CAAAAGGAAA AGGAATACTC ATGACAGTGA GATGTTGGGT 
CTCAGGAGGC TATCCAGTAA AAAGAACATA AGAAAACAGC GAATGAAAAT TTTATTCAAT 
GTTGTTCTTG AAGCTCGAGA GCCAGGTTCA GGCAGAAGAC TTTGCGATCT ATTTATGGTT 
AAGCCATCCA AGAAGGACTA TCCTGATTAT TATAAAATCA TCTTAGAGCC AATGGACCTG 
AAAATAATTG AGCATAACAT CCGAAATGAC AAATATGCAG GTGAAGAAGG AATGATGGAA 
GACATGAAAC TCATGTTCCG CAATGCCAGG CACTACAATG AGGAGGGCTC CCAGGTATAC 
AATGATGCCC ATATCCTGGA GAAGTTACTC AAAGATAAAA GGAAAGAGCT GGGCCCTCTG 
CCTGATGATG ATGACATGGC TTCTCCCAAA CTTAAATTGA GTAGGAAGAG TGGTGTTTCT 
CCTAAGAAAT CAAAGTACAT GACTCCAATG CAGCAGAAAC TGAATGAAGT GTATGAAGCT 
GTAAAGAACT ATACTGATAA GAGGGGTCGC CGCCTTAGTG CTATATTTCT AAGACTCCCC 
TCTAGATCAG AGCTGCCTGA CTACTACCTG ACCATTAAAA AGCCCATGGA CATGGAAAAA 
ATTCGAAGTC ACATGATGGC AAACAAGTAC CAAGACATAG ATTCTATGGT AGAGGACTTT 
GTCATGATGT TTAATAATGC CTGTACCTAC AATGAACCAG AGTCTTTGAT CTACAAAGAT 
GCCCTTGTAC TGCATAAAGT CCTCCTTGAG ACTCGGAGAG ACCTGGAGGG AGATGAGGAT 
TCTCATGTCC CTAATGTGAC GTTGCTGATT CAAGAGCTCA TCCATAACCT TTTTGTGTCA 
GTCATGAGTC ATCAGGATGA CGAAGGGAGG TGTTACAGCG ACTCCTTAGC AGAAATTCCT 
GCTGTGGATC CCAACTCTCC CAATAAACCT CCCCTTACAT TTGACATTAT CAGGAAAAAT 
GTTGAAAGTA ATCGGTATCG GCGACTTGAT TTATTTCAGG AGCATATGTT TGAAGTATTG 
GAACGGGCAA GAAGGATGAA CCGGACAGAT TCCGAAATAT ATGAGGATGC TGTAGAACTT 
CAGCAGTTTT TTATTAGAAT TCGTGATGAA CTCTGCAAAA ATGGAGAGAT CCTTCTTTCT 
CCAGCACTCA GCTATACCAC AAAACACTTG CATAACGATG TGGAAAAAGA AAAAAAGGAA 
AAATTGCCTA AAGAAATAGA GGAAGATAAA CTAAAACGCG AAGAAGAAAA AAGAGAAGCT 
GAAAAAAGTG AAGATTCCTC AGGTACTACA GGCCTCTCAG GCTTACATCG TACATACAGC 
CAGGACTGCA GCTTTAAGAA CAGCATGTAT CATGTCGGAG ATTATGTCTA TGTTGAACCT 
GCGGAGGCCA ATCTACAACC ACATATAGTG TGTATTGAGA GACTGTGGGA GGATTCAGCT 
GGTGAAAAAT GGTTGTACGG CTGTTGGTTT TATCGGCCAA ATGAAACATT CCATTTGGCT 
ACACGAAAAT TTCTAGAAAA AGAAGTTTTT AAGAGTGACT ACTACAATAA AGTACCTGTT 
AGTAAAATTC TAGGCAAATG TGTAGTCATG TTTGTCAAGG AATACTTTAA ATTATGTCCA 
GAAAACTTTC GCGATGAGGA TGTTTTTGTC TGTGAATCGA GGTATTCTGC CAAAACCAAA 
TCTTTTAAGA AAATTAAACT GTGGACCATG CCCATCAGTT CAGTTAGATT TGTCCCTCGG 
GATGTGCCTT TGCCTGTGGT CCGAGTGGCC TCTGTGTTTG CAAATGCAGA TAAAGGGGAT 
GATGAGAAGA ATACAGACAA CTCAGATGAC AATAGAGCTG AAGACAATTT TAACTTGGAA 
AAGGAAAAAG AAGATGTTCC TGTGGAGATG TCCAATGGTG AGCCAGGTTG CCACTACTTT 
GAGCAGCTTC GGTACAATGA CATGTGGCTG AAGGTTGGTG ATTGTGTCTT CATCAAATCC 
CACGGCTTGG TGCGCCCTCG TGTGGGCAGA ATTGAGAAAG TATGGGTCCG AGATGGAGCT 
GCATATTTTT ATGGCCCTAT CTTCATTCAT CCAGAAGAAA CAGAACATGA GCCCACAAAA 
ATGTTCTACA AAAAAGAAGT GTTTCTGAGT AATCTGGAAG AGACCTGCCC TATGAGTTGT 
ATTCTGGGGA AATGTGCAGT GCTGTCATTC AAGGACTTCC TCTCCTGCAG GCCAACTGAA 
ATACCAGAAA ATGACATTCT GCTTTGTGAG AGCCGCTATA ATGAGAGTGA CAAGCAGATG 
AAGAAGTTCA AGGGTTTGAA GAGGTTTTCA CTCTCTGCTA AAGTTGTAGA TGATGAAATC 
TACTACTTCA GAAAACCAAT CATTCCTCAG AAGGAACCCT CACCTTTGTT AGAAAAGAAG 
ATACAATTGC TAGAAGCTAA ATTTGCAGAG TTAGAAGGAG GAGATGATGA TATTGAGGAG 
ATGGGAGAAG AGGATAGTGA AGTCATTGAA GCTCCATCTC TACCTCAACT GCAGACACCC 
CTGGCCAATG AGTTGGACCT CATGCCCTAT ACACCCCCAC AGCTTTCTGT CTTACAGTCT 
ACCCCAAAGT CTGCCAAAGG CAGTGCAAAG AAGGAAAGTT CTAAACGAAA AATCAACATG 
AGTGGCTACA TTTTGTTCAG CAGTGAAATG AGAGCTGTGA TTAAAGCCCA GCACCCAGAC 
TACTCTTTTG GGGAGCTCAG CAGACTGGTG GGGACAGAAT GGAGAAACCT TGAAACAGCC 
AAGAAAGCAG AATATGAAGA GCGGGCAGCT AAAGTTGCTG AGCAGCAGGA GAGAGAGCGA 
GCAGCACAGC AACAGCAGCC GAGTGCTTCT CCCCGAGCAG GCACCCCTGT GGGGGCTCTC 
ATGGGGGTGG TGCCACCACC AACACCAATG GGGATGCTCA ATCAGCAGTT GACACCTGTT 
GCAGGCATGA TGGGTGGCTA TCCGCCAGGC CTTCCACCTT TGCAGGGCCC AGTTGATGGC 
CTTGTTAGCA TGGGCAGCAT GCAGCCACTT CACCCTGGGG GGCCTCCACC TCACCATCTT 
CCGCCAGGTG TGCCTGGCCT CCCAGGCATC CCACCACCGG GTGTGATGAA TCAAGGAGTA 
GCCCCCATGG TAGGGACTCC AGCACCAGGT GGAAGTCCGT ATGGACAACA GGTAGGAGTT 
TTGGGACCTC CAGGGCAGCA GGCACCACCT CCATATCCTG GTCCTCATCC AGCTGGCCCC 
CCTGTCATAC AGCAGCCAAC AACGCCCATG TTTGTGGCTC CCCCACCAAA GACCCAAAGG 
CTTCTCCACT CAGAGGCCTA CCTGAAATAC ATTGAAGGAC TCAGTGCTGA ATCCAACAGC 
ATTAGCAAGT GGGACCAAAC TTTGGCAGCT CGAAGACGGG ATGTCCATTT GTCCAAAGAA 
CAGGAGAGCC GCCTACCTTC TCACTGGCTC AAAAGTAAAG GGGCACACAC CACCATGGCA 
GATGCCCTCT GGCGCCTACG GGATTTAATG CTTCGAGACA CTCTCAACAT CCGACAGGCA 
TACAACCTAG AAAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171631.1
CDS	443..5623
Translation	MRRLAFRGAGCALKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316142.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316142.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGA AGTTGGATTC CATGGGTTCC 
AAGAGAAGAA GAGCCACCTC TCCTTCCAGC AGTGTCAGTG GAGACTTTGA TGACGGGCAC 
CATTCTGTGC CTACACCAGG CCCAAGCAGG AAAAGGAGAA GACTGTCCAA TCTTCCAACT 
GTAGATCCTA TTGCTGTGTG CCATGAACTC TATAACACCA TCCGAGACTA TAAGGATGAA 
CAGGGCAGAC TCCTCTGTGA GCTGTTCATT AGGGCTCCAA AGCGGAGAAA TCAACCAGAC 
TATTATGAAG TGGTTTCTCA GCCCATTGAC TTGATGAAAA TCCAACAGAA ACTTAAAATG 
GAAGAGTATG ATGATGTTAA TCTACTGACT GCTGACTTCC AGCTGCTTTT TAACAATGCA 
AAGGCCTACT ATAAGCCAGA TTCCCCTGAG TATAAAGCTG CTTGTAAACT CTGGGATTTG 
TACCTTCGAA CAAGAAATGA GTTTGTTCAG AAAGGAGAAG CAGACGATGA AGATGATGAC 
GAAGATGGGC AAGACAATCA AGGCACACTG GCTGACGGCT CTTCTCCAGG TTATCTGAAG 
GAGATCCTGG AGCAGCTTCT TGAAGCCATA GTTGTAGCCA CAAATCCATC AGGACGGCTC 
ATCAGTGAAC TTTTTCAGAA ACTGCCTTCC AAAGTGCAAT ATCCAGACTA TTATGCAATA 
ATTAAGGAAC CTATAGATCT CAAGACCATT GCTCAGAGGA TACAGAATGG AAGCTACAAA 
AGTATACACG CAATGGCCAA AGATATAGAT CTTCTAGCAA AAAATGCCAA AACATACAAT 
GAGCCTGGGT CTCAAGTATT CAAGGATGCC AATTCGATTA AAAAAATATT TTATATGAAA 
AAGGCAGAAA TTGAACATCA TGAAATGACT AAATCAAGTC TTCGAATAAG GACTGCATCA 
AATTTGGCTG CAGCCAGGCT GACAGGTCCT TCGCACAATA AAAGCAGCCT TGGTGAAGAA 
AGAAACCCCA CTAGCAAGTA TTACCGTAAT AAAAGAGCAG TCCAAGGGGG TCGCTTGTCA 
GCAATTACCA TGGCACTTCA GTATGGATCA GAGAGTGAAG AGGACGCTGC TTTAGCTGCT 
GCACGCTATG AAGAAGGGGA ATCTGAAGCA GAGAGCATCA CTTCCTTCAT GGACGTTTCC 
AACCCCTTTC ATCAGCTTTA CGACACAGTT AGGAGCTGTA GGAATCACCA AGGGCAGCTC 
ATAGCTGAAC CTTTCTTCCA TTTGCCTTCA AAGAAAAAAT ACCCAGATTA TTATCAGCAA 
ATTAAAATGC CCATATCACT TCAACAGATC AGAACAAAGC TAAAGAACCA AGAATATGAA 
ACTTTAGATC ATTTGGAGTG TGATCTGAAT TTAATGTTTG AAAATGCCAA ACGTTATAAC 
GTTCCCAATT CAGCCATCTA TAAGCGAGTT CTAAAACTGC AGCAAGTCAT GCAGGCAAAG 
AAGAAGGAGC TTGCGAGGAG AGATGACATT GAGGACGGAG ACAGCATGAT CTCCTCAGCC 
ACTTCTGACA CTGGTAGTGC CAAAAGGAAA AGGAATACTC ATGACAGTGA GATGTTGGGT 
CTCAGGAGGC TATCCAGTAA AAAGAACATA AGAAAACAGC GAATGAAAAT TTTATTCAAT 
GTTGTTCTTG AAGCTCGAGA GCCAGGTTCA GGCAGAAGAC TTTGCGATCT ATTTATGGTT 
AAGCCATCCA AGAAGGACTA TCCTGATTAT TATAAAATCA TCTTAGAGCC AATGGACCTG 
AAAATAATTG AGCATAACAT CCGAAATGAC AAATATGCAG GTGAAGAAGG AATGATGGAA 
GACATGAAAC TCATGTTCCG CAATGCCAGG CACTACAATG AGGAGGGCTC CCAGGTATAC 
AATGATGCCC ATATCCTGGA GAAGTTACTC AAAGATAAAA GGAAAGAGCT GGGCCCTCTG 
CCTGATGATG ATGACATGGC TTCTCCCAAA CTTAAATTGA GTAGGAAGAG TGGTGTTTCT 
CCTAAGAAAT CAAAGTACAT GACTCCAATG CAGCAGAAAC TGAATGAAGT GTATGAAGCT 
GTAAAGAACT ATACTGATAA GAGGGGTCGC CGCCTTAGTG CTATATTTCT AAGACTCCCC 
TCTAGATCAG AGCTGCCTGA CTACTACCTG ACCATTAAAA AGCCCATGGA CATGGAAAAA 
ATTCGAAGTC ACATGATGGC AAACAAGTAC CAAGACATAG ATTCTATGGT AGAGGACTTT 
GTCATGATGT TTAATAATGC CTGTACCTAC AATGAACCAG AGTCTTTGAT CTACAAAGAT 
GCCCTTGTAC TGCATAAAGT CCTCCTTGAG ACTCGGAGAG ACCTGGAGGG AGATGAGGAT 
TCTCATGTCC CTAATGTGAC GTTGCTGATT CAAGAGCTCA TCCATAACCT TTTTGTGTCA 
GTCATGAGTC ATCAGGATGA CGAAGGGAGG TGTTACAGCG ACTCCTTAGC AGAAATTCCT 
GCTGTGGATC CCAACTCTCC CAATAAACCT CCCCTTACAT TTGACATTAT CAGGAAAAAT 
GTTGAAAGTA ATCGGTATCG GCGACTTGAT TTATTTCAGG AGCATATGTT TGAAGTATTG 
GAACGGGCAA GAAGGATGAA CCGGACAGAT TCCGAAATAT ATGAGGATGC TGTAGAACTT 
CAGCAGTTTT TTATTAGAAT TCGTGATGAA CTCTGCAAAA ATGGAGAGAT CCTTCTTTCT 
CCAGCACTCA GCTATACCAC AAAACACTTG CATAACGATG TGGAAAAAGA AAAAAAGGAA 
AAATTGCCTA AAGAAATAGA GGAAGATAAA CTAAAACGCG AAGAAGAAAA AAGAGAAGCT 
GAAAAAAGTG AAGATTCCTC AGGTACTACA GGCCTCTCAG GCTTACATCG TACATACAGC 
CAGGACTGCA GCTTTAAGAA CAGCATGTAT CATGTCGGAG ATTATGTCTA TGTTGAACCT 
GCGGAGGCCA ATCTACAACC ACATATAGTG TGTATTGAGA GACTGTGGGA GGATTCAGCT 
GGTGAAAAAT GGTTGTACGG CTGTTGGTTT TATCGGCCAA ATGAAACATT CCATTTGGCT 
ACACGAAAAT TTCTAGAAAA AGAAGTTTTT AAGAGTGACT ACTACAATAA AGTACCTGTT 
AGTAAAATTC TAGGCAAATG TGTAGTCATG TTTGTCAAGG AATACTTTAA ATTATGTCCA 
GAAAACTTTC GCGATGAGGA TGTTTTTGTC TGTGAATCGA GGTATTCTGC CAAAACCAAA 
TCTTTTAAGA AAATTAAACT GTGGACCATG CCCATCAGTT CAGTTAGATT TGTCCCTCGG 
GATGTGCCTT TGCCTGTGGT CCGAGTGGCC TCTGTGTTTG CAAATGCAGA TAAAGGGGAT 
GATGAGAAGA ATACAGACAA CTCAGATGAC AATAGAGCTG AAGACAATTT TAACTTGGAA 
AAGGAAAAAG AAGATGTTCC TGTGGAGATG TCCAATGGTG AGCCAGGTTG CCACTACTTT 
GAGCAGCTTC GGTACAATGA CATGTGGCTG AAGGTTGGTG ATTGTGTCTT CATCAAATCC 
CACGGCTTGG TGCGCCCTCG TGTGGGCAGA ATTGAGAAAG TATGGGTCCG AGATGGAGCT 
GCATATTTTT ATGGCCCTAT CTTCATTCAT CCAGAAGAAA CAGAACATGA GCCCACAAAA 
ATGTTCTACA AAAAAGAAGT GTTTCTGAGT AATCTGGAAG AGACCTGCCC TATGAGTTGT 
ATTCTGGGGA AATGTGCAGT GCTGTCATTC AAGGACTTCC TCTCCTGCAG GCCAACTGAA 
ATACCAGAAA ATGACATTCT GCTTTGTGAG AGCCGCTATA ATGAGAGTGA CAAGCAGATG 
AAGAAGTTCA AGGGTTTGAA GAGGTTTTCA CTCTCTGCTA AAGTTGTAGA TGATGAAATC 
TACTACTTCA GAAAACCAAT CATTCCTCAG AAGGAACCCT CACCTTTGTT AGAAAAGAAG 
ATACAATTGC TAGAAGCTAA ATTTGCAGAG TTAGAAGGAG GAGATGATGA TATTGAGGAG 
ATGGGAGAAG AGGATAGTGA AGTCATTGAA GCTCCATCTC TACCTCAACT GCAGACACCC 
CTGGCCAATG AGTTGGACCT CATGCCCTAT ACACCCCCAC AGCTTTCTGT CTTACAGTCT 
ACCCCAAAGT CTGCCAAAGG CAGTGCAAAG AAGGAAAGTT CTAAACGAAA AATCAACATG 
AGTGGCTACA TTTTGTTCAG CAGTGAAATG AGAGCTGTGA TTAAAGCCCA GCACCCAGAC 
TACTCTTTTG GGGAGCTCAG CAGACTGGTG GGGACAGAAT GGAGAAACCT TGAAACAGCC 
AAGAAAGCAG AATATGAAGA GCGGGCAGCT AAAGTTGCTG AGCAGCAGGA GAGAGAGCGA 
GCAGCACAGC AACAGCAGCC GAGTGCTTCT CCCCGAGCAG GCACCCCTGT GGGGGCTCTC 
ATGGGGGTGG TGCCACCACC AACACCAATG GGGATGCTCA ATCAGCAGTT GACACCTGTT 
GCAGGCATGA TGGGTGGCTA TCCGCCAGGC CTTCCACCTT TGCAGGGCCC AGTTGATGGC 
CTTGTTAGCA TGGGCAGCAT GCAGCCACTT CACCCTGGGG GGCCTCCACC TCACCATCTT 
CCGCCAGGTG TGCCTGGCCT CCCAGGCATC CCACCACCGG GTGTGATGAA TCAAGGAGTA 
GCCCCCATGG TAGGGACTCC AGCACCAGGT GGAAGTCCGT ATGGACAACA GGTAGGAGTT 
TTGGGACCTC CAGGGCAGCA GGCACCACCT CCATATCCTG GTCCTCATCC AGCTGGCCCC 
CCTGTCATAC AGCAGCCAAC AACGCCCATG TTTGTGGCTC CCCCACCAAA GACCCAAAGG 
CTTCTCCACT CAGAGGCCTA CCTGAAATAC ATTGAAGGAC TCAGTGCTGA ATCCAACAGC 
ATTAGCAAGT GGGACCAAAC TTTGGCAGCT CGAAGACGGG ATGTCCATTT GTCCAAAGAA 
CAGGAGAGCC GCCTACCTTC TCACTGGCTC AAAAGTAAAG GGGCACACAC CACCATGGCA 
GATGCCCTCT GGCGCCTACG GGATTTAATG CTTCGAGACA CTCTCAACAT CCGACAGGCA 
TACAACCTAG AAAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519409.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519472.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X13, mRNA.

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CloneID	OMu50257
Clone ID Related Accession (Same CDS sequence)	XM_006519409.3
Accession Version	XM_006519409.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519409.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGTCTACC 
CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG GAAAGTTCTA AACGAAAAAT CAACATGAGT 
GGCTACATTT TGTTCAGCAG TGAAATGAGA GCTGTGATTA AAGCCCAGCA CCCAGACTAC 
TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG ACAGAATGGA GAAACCTTGA AACAGCCAAG 
AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA 
GCACAGCAAC AGCAGCCGAG TGCTTCTCCC CGAGCAGGCA CCCCTGTGGG GGCTCTCATG 
GGGGTGGTGC CACCACCAAC ACCAATGGGG ATGCTCAATC AGCAGTTGAC ACCTGTTGCA 
GGCATGATGG GTGGCTATCC GCCAGGCCTT CCACCTTTGC AGGGCCCAGT TGATGGCCTT 
GTTAGCATGG GCAGCATGCA GCCACTTCAC CCTGGGGGGC CTCCACCTCA CCATCTTCCG 
CCAGGTGTGC CTGGCCTCCC AGGCATCCCA CCACCGGGTG TGATGAATCA AGGAGTAGCC 
CCCATGGTAG GGACTCCAGC ACCAGGTGGA AGTCCGTATG GACAACAGGT AGGAGTTTTG 
GGACCTCCAG GGCAGCAGGC ACCACCTCCA TATCCTGGTC CTCATCCAGC TGGCCCCCCT 
GTCATACAGC AGCCAACAAC GCCCATGTTT GTGGCTCCCC CACCAAAGAC CCAAAGGCTT 
CTCCACTCAG AGGCCTACCT GAAATACATT GAAGGACTCA GTGCTGAATC CAACAGCATT 
AGCAAGTGGG ACCAAACTTT GGCAGCTCGA AGACGGGATG TCCATTTGTC CAAAGAACAG 
GAGAGCCGCC TACCTTCTCA CTGGCTCAAA AGTAAAGGGG CACACACCAC CATGGCAGAT 
GCCCTCTGGC GCCTACGGGA TTTAATGCTT CGAGACACTC TCAACATCCG ACAGGCATAC 
AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519472.1
CDS	345..5522
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519409.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519409.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGTCTACC 
CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG GAAAGTTCTA AACGAAAAAT CAACATGAGT 
GGCTACATTT TGTTCAGCAG TGAAATGAGA GCTGTGATTA AAGCCCAGCA CCCAGACTAC 
TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG ACAGAATGGA GAAACCTTGA AACAGCCAAG 
AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA 
GCACAGCAAC AGCAGCCGAG TGCTTCTCCC CGAGCAGGCA CCCCTGTGGG GGCTCTCATG 
GGGGTGGTGC CACCACCAAC ACCAATGGGG ATGCTCAATC AGCAGTTGAC ACCTGTTGCA 
GGCATGATGG GTGGCTATCC GCCAGGCCTT CCACCTTTGC AGGGCCCAGT TGATGGCCTT 
GTTAGCATGG GCAGCATGCA GCCACTTCAC CCTGGGGGGC CTCCACCTCA CCATCTTCCG 
CCAGGTGTGC CTGGCCTCCC AGGCATCCCA CCACCGGGTG TGATGAATCA AGGAGTAGCC 
CCCATGGTAG GGACTCCAGC ACCAGGTGGA AGTCCGTATG GACAACAGGT AGGAGTTTTG 
GGACCTCCAG GGCAGCAGGC ACCACCTCCA TATCCTGGTC CTCATCCAGC TGGCCCCCCT 
GTCATACAGC AGCCAACAAC GCCCATGTTT GTGGCTCCCC CACCAAAGAC CCAAAGGCTT 
CTCCACTCAG AGGCCTACCT GAAATACATT GAAGGACTCA GTGCTGAATC CAACAGCATT 
AGCAAGTGGG ACCAAACTTT GGCAGCTCGA AGACGGGATG TCCATTTGTC CAAAGAACAG 
GAGAGCCGCC TACCTTCTCA CTGGCTCAAA AGTAAAGGGG CACACACCAC CATGGCAGAT 
GCCCTCTGGC GCCTACGGGA TTTAATGCTT CGAGACACTC TCAACATCCG ACAGGCATAC 
AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316143.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171632.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X15, mRNA.

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CloneID	OMu50258
Clone ID Related Accession (Same CDS sequence)	XM_017316143.1 , XM_006519410.3 , XM_017316144.1
Accession Version	XM_017316143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGC TTTCTGTCTT ACAGTCTACC CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG 
GAAAGTTCTA AACGAAAAAT CAACATGAGT GGCTACATTT TGTTCAGCAG TGAAATGAGA 
GCTGTGATTA AAGCCCAGCA CCCAGACTAC TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG 
ACAGAATGGA GAAACCTTGA AACAGCCAAG AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA 
GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA GCACAGCAAC AGCAGCCGAG TGCTTCTCCC 
CGAGCAGGCA CCCCTGTGGG GGCTCTCATG GGGGTGGTGC CACCACCAAC ACCAATGGGG 
ATGCTCAATC AGCAGTTGAC ACCTGTTGCA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171632.1
CDS	443..5590
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316143.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316143.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGC TTTCTGTCTT ACAGTCTACC CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG 
GAAAGTTCTA AACGAAAAAT CAACATGAGT GGCTACATTT TGTTCAGCAG TGAAATGAGA 
GCTGTGATTA AAGCCCAGCA CCCAGACTAC TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG 
ACAGAATGGA GAAACCTTGA AACAGCCAAG AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA 
GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA GCACAGCAAC AGCAGCCGAG TGCTTCTCCC 
CGAGCAGGCA CCCCTGTGGG GGCTCTCATG GGGGTGGTGC CACCACCAAC ACCAATGGGG 
ATGCTCAATC AGCAGTTGAC ACCTGTTGCA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519410.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519473.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X14, mRNA.

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CloneID	OMu50258
Clone ID Related Accession (Same CDS sequence)	XM_017316143.1 , XM_006519410.3 , XM_017316144.1
Accession Version	XM_006519410.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519410.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGC TTTCTGTCTT ACAGTCTACC CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG 
GAAAGTTCTA AACGAAAAAT CAACATGAGT GGCTACATTT TGTTCAGCAG TGAAATGAGA 
GCTGTGATTA AAGCCCAGCA CCCAGACTAC TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG 
ACAGAATGGA GAAACCTTGA AACAGCCAAG AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA 
GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA GCACAGCAAC AGCAGCCGAG TGCTTCTCCC 
CGAGCAGGCA CCCCTGTGGG GGCTCTCATG GGGGTGGTGC CACCACCAAC ACCAATGGGG 
ATGCTCAATC AGCAGTTGAC ACCTGTTGCA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519473.1
CDS	359..5506
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519410.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519410.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGC TTTCTGTCTT ACAGTCTACC CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG 
GAAAGTTCTA AACGAAAAAT CAACATGAGT GGCTACATTT TGTTCAGCAG TGAAATGAGA 
GCTGTGATTA AAGCCCAGCA CCCAGACTAC TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG 
ACAGAATGGA GAAACCTTGA AACAGCCAAG AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA 
GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA GCACAGCAAC AGCAGCCGAG TGCTTCTCCC 
CGAGCAGGCA CCCCTGTGGG GGCTCTCATG GGGGTGGTGC CACCACCAAC ACCAATGGGG 
ATGCTCAATC AGCAGTTGAC ACCTGTTGCA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316144.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171633.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X16, mRNA.

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CloneID	OMu50258
Clone ID Related Accession (Same CDS sequence)	XM_017316143.1 , XM_006519410.3 , XM_017316144.1
Accession Version	XM_017316144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGC TTTCTGTCTT ACAGTCTACC CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG 
GAAAGTTCTA AACGAAAAAT CAACATGAGT GGCTACATTT TGTTCAGCAG TGAAATGAGA 
GCTGTGATTA AAGCCCAGCA CCCAGACTAC TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG 
ACAGAATGGA GAAACCTTGA AACAGCCAAG AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA 
GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA GCACAGCAAC AGCAGCCGAG TGCTTCTCCC 
CGAGCAGGCA CCCCTGTGGG GGCTCTCATG GGGGTGGTGC CACCACCAAC ACCAATGGGG 
ATGCTCAATC AGCAGTTGAC ACCTGTTGCA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171633.1
CDS	202..5349
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316144.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316144.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGC TTTCTGTCTT ACAGTCTACC CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG 
GAAAGTTCTA AACGAAAAAT CAACATGAGT GGCTACATTT TGTTCAGCAG TGAAATGAGA 
GCTGTGATTA AAGCCCAGCA CCCAGACTAC TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG 
ACAGAATGGA GAAACCTTGA AACAGCCAAG AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA 
GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA GCACAGCAAC AGCAGCCGAG TGCTTCTCCC 
CGAGCAGGCA CCCCTGTGGG GGCTCTCATG GGGGTGGTGC CACCACCAAC ACCAATGGGG 
ATGCTCAATC AGCAGTTGAC ACCTGTTGCA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519411.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519474.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X18, mRNA.

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CloneID	OMu50259
Clone ID Related Accession (Same CDS sequence)	XM_006519411.3
Accession Version	XM_006519411.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519411.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519474.1
CDS	358..5490
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519411.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519411.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519414.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519477.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X22, mRNA.

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CloneID	OMu50260
Clone ID Related Accession (Same CDS sequence)	XM_006519414.3 , XM_017316146.1 , XM_006519413.3 , XM_006519412.3
Accession Version	XM_006519414.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519414.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519477.1
CDS	169..5298
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519414.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519414.3

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316146.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171635.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OMu50260
Clone ID Related Accession (Same CDS sequence)	XM_006519414.3 , XM_017316146.1 , XM_006519413.3 , XM_006519412.3
Accession Version	XM_017316146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171635.1
CDS	386..5515
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316146.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316146.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519413.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519476.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X21, mRNA.

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CloneID	OMu50260
Clone ID Related Accession (Same CDS sequence)	XM_006519414.3 , XM_017316146.1 , XM_006519413.3 , XM_006519412.3
Accession Version	XM_006519413.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519413.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519476.1
CDS	306..5435
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519413.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519413.3

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519412.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519475.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X19, mRNA.

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CloneID	OMu50260
Clone ID Related Accession (Same CDS sequence)	XM_006519414.3 , XM_017316146.1 , XM_006519413.3 , XM_006519412.3
Accession Version	XM_006519412.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519412.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519475.1
CDS	309..5438
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519412.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519412.3

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GGAATACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAACAGCG AATGAAAATT 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGCGATCTA 
TTTATGGTTA AGCCATCCAA GAAGGACTAT CCTGATTATT ATAAAATCAT CTTAGAGCCA 
ATGGACCTGA AAATAATTGA GCATAACATC CGAAATGACA AATATGCAGG TGAAGAAGGA 
ATGATGGAAG ACATGAAACT CATGTTCCGC AATGCCAGGC ACTACAATGA GGAGGGCTCC 
CAGGTATACA ATGATGCCCA TATCCTGGAG AAGTTACTCA AAGATAAAAG GAAAGAGCTG 
GGCCCTCTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TTAAATTGAG TAGGAAGAGT 
GGTGTTTCTC CTAAGAAATC AAAGTACATG ACTCCAATGC AGCAGAAACT GAATGAAGTG 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTTAGTGC TATATTTCTA 
AGACTCCCCT CTAGATCAGA GCTGCCTGAC TACTACCTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCA AACAAGTACC AAGACATAGA TTCTATGGTA 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACCTACA ATGAACCAGA GTCTTTGATC 
TACAAAGATG CCCTTGTACT GCATAAAGTC CTCCTTGAGA CTCGGAGAGA CCTGGAGGGA 
GATGAGGATT CTCATGTCCC TAATGTGACG TTGCTGATTC AAGAGCTCAT CCATAACCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAC GAAGGGAGGT GTTACAGCGA CTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTCTCCC AATAAACCTC CCCTTACATT TGACATTATC 
AGGAAAAATG TTGAAAGTAA TCGGTATCGG CGACTTGATT TATTTCAGGA GCATATGTTT 
GAAGTATTGG AACGGGCAAG AAGGATGAAC CGGACAGATT CCGAAATATA TGAGGATGCT 
GTAGAACTTC AGCAGTTTTT TATTAGAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCTC CAGCACTCAG CTATACCACA AAACACTTGC ATAACGATGT GGAAAAAGAA 
AAAAAGGAAA AATTGCCTAA AGAAATAGAG GAAGATAAAC TAAAACGCGA AGAAGAAAAA 
AGAGAAGCTG AAAAAAGTGA AGATTCCTCA GGTACTACAG GCCTCTCAGG CTTACATCGT 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTATC ATGTCGGAGA TTATGTCTAT 
GTTGAACCTG CGGAGGCCAA TCTACAACCA CATATAGTGT GTATTGAGAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTACGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA CTACAATAAA 
GTACCTGTTA GTAAAATTCT AGGCAAATGT GTAGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGTCCAG AAAACTTTCG CGATGAGGAT GTTTTTGTCT GTGAATCGAG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGTTC AGTTAGATTT 
GTCCCTCGGG ATGTGCCTTT GCCTGTGGTC CGAGTGGCCT CTGTGTTTGC AAATGCAGAT 
AAAGGGGATG ATGAGAAGAA TACAGACAAC TCAGATGACA ATAGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTTCCT GTGGAGATGT CCAATGGTGA GCCAGGTTGC 
CACTACTTTG AGCAGCTTCG GTACAATGAC ATGTGGCTGA AGGTTGGTGA TTGTGTCTTC 
ATCAAATCCC ACGGCTTGGT GCGCCCTCGT GTGGGCAGAA TTGAGAAAGT ATGGGTCCGA 
GATGGAGCTG CATATTTTTA TGGCCCTATC TTCATTCATC CAGAAGAAAC AGAACATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTG TTTCTGAGTA ATCTGGAAGA GACCTGCCCT 
ATGAGTTGTA TTCTGGGGAA ATGTGCAGTG CTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTATAA TGAGAGTGAC 
AAGCAGATGA AGAAGTTCAA GGGTTTGAAG AGGTTTTCAC TCTCTGCTAA AGTTGTAGAT 
GATGAAATCT ACTACTTCAG AAAACCAATC ATTCCTCAGA AGGAACCCTC ACCTTTGTTA 
GAAAAGAAGA TACAATTGCT AGAAGCTAAA TTTGCAGAGT TAGAAGGAGG AGATGATGAT 
ATTGAGGAGA TGGGAGAAGA GGATAGTGAA GTCATTGAAG CTCCATCTCT ACCTCAACTG 
CAGACACCCC TGGCCAATGA GTTGGACCTC ATGCCCTATA CACCCCCACA GCTTTCTGTC 
TTACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAAGTTC TAAACGAAAA 
ATCAACATGA GTGGCTACAT TTTGTTCAGC AGTGAAATGA GAGCTGTGAT TAAAGCCCAG 
CACCCAGACT ACTCTTTTGG GGAGCTCAGC AGACTGGTGG GGACAGAATG GAGAAACCTT 
GAAACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCT 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCAGGCATCC CACCACCGGG TGTGATGAAT 
CAAGGAGTAG CCCCCATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAACAG 
GTAGGAGTTT TGGGACCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG TCCTCATCCA 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACGCCCATGT TTGTGGCTCC CCCACCAAAG 
ACCCAAAGGC TTCTCCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCTGAA 
TCCAACAGCA TTAGCAAGTG GGACCAAACT TTGGCAGCTC GAAGACGGGA TGTCCATTTG 
TCCAAAGAAC AGGAGAGCCG CCTACCTTCT CACTGGCTCA AAAGTAAAGG GGCACACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTACGG GATTTAATGC TTCGAGACAC TCTCAACATC 
CGACAGGCAT ACAACCTAGA AAATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_017316147.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171636.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X24, mRNA.

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CloneID	OMu50261
Clone ID Related Accession (Same CDS sequence)	XM_017316147.1 , XM_006519415.3
Accession Version	XM_017316147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGGA ATACTCATGA CAGTGAGATG TTGGGTCTCA GGAGGCTATC CAGTAAAAAG 
AACATAAGAA AACAGCGAAT GAAAATTTTA TTCAATGTTG TTCTTGAAGC TCGAGAGCCA 
GGTTCAGGCA GAAGACTTTG CGATCTATTT ATGGTTAAGC CATCCAAGAA GGACTATCCT 
GATTATTATA AAATCATCTT AGAGCCAATG GACCTGAAAA TAATTGAGCA TAACATCCGA 
AATGACAAAT ATGCAGGTGA AGAAGGAATG ATGGAAGACA TGAAACTCAT GTTCCGCAAT 
GCCAGGCACT ACAATGAGGA GGGCTCCCAG GTATACAATG ATGCCCATAT CCTGGAGAAG 
TTACTCAAAG ATAAAAGGAA AGAGCTGGGC CCTCTGCCTG ATGATGATGA CATGGCTTCT 
CCCAAACTTA AATTGAGTAG GAAGAGTGGT GTTTCTCCTA AGAAATCAAA GTACATGACT 
CCAATGCAGC AGAAACTGAA TGAAGTGTAT GAAGCTGTAA AGAACTATAC TGATAAGAGG 
GGTCGCCGCC TTAGTGCTAT ATTTCTAAGA CTCCCCTCTA GATCAGAGCT GCCTGACTAC 
TACCTGACCA TTAAAAAGCC CATGGACATG GAAAAAATTC GAAGTCACAT GATGGCAAAC 
AAGTACCAAG ACATAGATTC TATGGTAGAG GACTTTGTCA TGATGTTTAA TAATGCCTGT 
ACCTACAATG AACCAGAGTC TTTGATCTAC AAAGATGCCC TTGTACTGCA TAAAGTCCTC 
CTTGAGACTC GGAGAGACCT GGAGGGAGAT GAGGATTCTC ATGTCCCTAA TGTGACGTTG 
CTGATTCAAG AGCTCATCCA TAACCTTTTT GTGTCAGTCA TGAGTCATCA GGATGACGAA 
GGGAGGTGTT ACAGCGACTC CTTAGCAGAA ATTCCTGCTG TGGATCCCAA CTCTCCCAAT 
AAACCTCCCC TTACATTTGA CATTATCAGG AAAAATGTTG AAAGTAATCG GTATCGGCGA 
CTTGATTTAT TTCAGGAGCA TATGTTTGAA GTATTGGAAC GGGCAAGAAG GATGAACCGG 
ACAGATTCCG AAATATATGA GGATGCTGTA GAACTTCAGC AGTTTTTTAT TAGAATTCGT 
GATGAACTCT GCAAAAATGG AGAGATCCTT CTTTCTCCAG CACTCAGCTA TACCACAAAA 
CACTTGCATA ACGATGTGGA AAAAGAAAAA AAGGAAAAAT TGCCTAAAGA AATAGAGGAA 
GATAAACTAA AACGCGAAGA AGAAAAAAGA GAAGCTGAAA AAAGTGAAGA TTCCTCAGGT 
ACTACAGGCC TCTCAGGCTT ACATCGTACA TACAGCCAGG ACTGCAGCTT TAAGAACAGC 
ATGTATCATG TCGGAGATTA TGTCTATGTT GAACCTGCGG AGGCCAATCT ACAACCACAT 
ATAGTGTGTA TTGAGAGACT GTGGGAGGAT TCAGCTGGTG AAAAATGGTT GTACGGCTGT 
TGGTTTTATC GGCCAAATGA AACATTCCAT TTGGCTACAC GAAAATTTCT AGAAAAAGAA 
GTTTTTAAGA GTGACTACTA CAATAAAGTA CCTGTTAGTA AAATTCTAGG CAAATGTGTA 
GTCATGTTTG TCAAGGAATA CTTTAAATTA TGTCCAGAAA ACTTTCGCGA TGAGGATGTT 
TTTGTCTGTG AATCGAGGTA TTCTGCCAAA ACCAAATCTT TTAAGAAAAT TAAACTGTGG 
ACCATGCCCA TCAGTTCAGT TAGATTTGTC CCTCGGGATG TGCCTTTGCC TGTGGTCCGA 
GTGGCCTCTG TGTTTGCAAA TGCAGATAAA GGGGATGATG AGAAGAATAC AGACAACTCA 
GATGACAATA GAGCTGAAGA CAATTTTAAC TTGGAAAAGG AAAAAGAAGA TGTTCCTGTG 
GAGATGTCCA ATGGTGAGCC AGGTTGCCAC TACTTTGAGC AGCTTCGGTA CAATGACATG 
TGGCTGAAGG TTGGTGATTG TGTCTTCATC AAATCCCACG GCTTGGTGCG CCCTCGTGTG 
GGCAGAATTG AGAAAGTATG GGTCCGAGAT GGAGCTGCAT ATTTTTATGG CCCTATCTTC 
ATTCATCCAG AAGAAACAGA ACATGAGCCC ACAAAAATGT TCTACAAAAA AGAAGTGTTT 
CTGAGTAATC TGGAAGAGAC CTGCCCTATG AGTTGTATTC TGGGGAAATG TGCAGTGCTG 
TCATTCAAGG ACTTCCTCTC CTGCAGGCCA ACTGAAATAC CAGAAAATGA CATTCTGCTT 
TGTGAGAGCC GCTATAATGA GAGTGACAAG CAGATGAAGA AGTTCAAGGG TTTGAAGAGG 
TTTTCACTCT CTGCTAAAGT TGTAGATGAT GAAATCTACT ACTTCAGAAA ACCAATCATT 
CCTCAGAAGG AACCCTCACC TTTGTTAGAA AAGAAGATAC AATTGCTAGA AGCTAAATTT 
GCAGAGTTAG AAGGAGGAGA TGATGATATT GAGGAGATGG GAGAAGAGGA TAGTGAAGTC 
ATTGAAGCTC CATCTCTACC TCAACTGCAG ACACCCCTGG CCAATGAGTT GGACCTCATG 
CCCTATACAC CCCCACAGCT TTCTGTCTTA CAGTCTACCC CAAAGTCTGC CAAAGGCAGT 
GCAAAGAAGG AAAGTTCTAA ACGAAAAATC AACATGAGTG GCTACATTTT GTTCAGCAGT 
GAAATGAGAG CTGTGATTAA AGCCCAGCAC CCAGACTACT CTTTTGGGGA GCTCAGCAGA 
CTGGTGGGGA CAGAATGGAG AAACCTTGAA ACAGCCAAGA AAGCAGAATA TGAAGAGCGG 
GCAGCTAAAG TTGCTGAGCA GCAGGAGAGA GAGCGAGCAG CACAGCAACA GCAGCCGAGT 
GCTTCTCCCC GAGCAGGCAC CCCTGTGGGG GCTCTCATGG GGGTGGTGCC ACCACCAACA 
CCAATGGGGA TGCTCAATCA GCAGTTGACA CCTGTTGCAG GCATGATGGG TGGCTATCCG 
CCAGGCCTTC CACCTTTGCA GGGCCCAGTT GATGGCCTTG TTAGCATGGG CAGCATGCAG 
CCACTTCACC CTGGGGGGCC TCCACCTCAC CATCTTCCGC CAGGTGTGCC TGGCCTCCCA 
GGCATCCCAC CACCGGGTGT GATGAATCAA GGAGTAGCCC CCATGGTAGG GACTCCAGCA 
CCAGGTGGAA GTCCGTATGG ACAACAGGTA GGAGTTTTGG GACCTCCAGG GCAGCAGGCA 
CCACCTCCAT ATCCTGGTCC TCATCCAGCT GGCCCCCCTG TCATACAGCA GCCAACAACG 
CCCATGTTTG TGGCTCCCCC ACCAAAGACC CAAAGGCTTC TCCACTCAGA GGCCTACCTG 
AAATACATTG AAGGACTCAG TGCTGAATCC AACAGCATTA GCAAGTGGGA CCAAACTTTG 
GCAGCTCGAA GACGGGATGT CCATTTGTCC AAAGAACAGG AGAGCCGCCT ACCTTCTCAC 
TGGCTCAAAA GTAAAGGGGC ACACACCACC ATGGCAGATG CCCTCTGGCG CCTACGGGAT 
TTAATGCTTC GAGACACTCT CAACATCCGA CAGGCATACA ACCTAGAAAA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171636.1
CDS	432..5528
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_017316147.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316147.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGGA ATACTCATGA CAGTGAGATG TTGGGTCTCA GGAGGCTATC CAGTAAAAAG 
AACATAAGAA AACAGCGAAT GAAAATTTTA TTCAATGTTG TTCTTGAAGC TCGAGAGCCA 
GGTTCAGGCA GAAGACTTTG CGATCTATTT ATGGTTAAGC CATCCAAGAA GGACTATCCT 
GATTATTATA AAATCATCTT AGAGCCAATG GACCTGAAAA TAATTGAGCA TAACATCCGA 
AATGACAAAT ATGCAGGTGA AGAAGGAATG ATGGAAGACA TGAAACTCAT GTTCCGCAAT 
GCCAGGCACT ACAATGAGGA GGGCTCCCAG GTATACAATG ATGCCCATAT CCTGGAGAAG 
TTACTCAAAG ATAAAAGGAA AGAGCTGGGC CCTCTGCCTG ATGATGATGA CATGGCTTCT 
CCCAAACTTA AATTGAGTAG GAAGAGTGGT GTTTCTCCTA AGAAATCAAA GTACATGACT 
CCAATGCAGC AGAAACTGAA TGAAGTGTAT GAAGCTGTAA AGAACTATAC TGATAAGAGG 
GGTCGCCGCC TTAGTGCTAT ATTTCTAAGA CTCCCCTCTA GATCAGAGCT GCCTGACTAC 
TACCTGACCA TTAAAAAGCC CATGGACATG GAAAAAATTC GAAGTCACAT GATGGCAAAC 
AAGTACCAAG ACATAGATTC TATGGTAGAG GACTTTGTCA TGATGTTTAA TAATGCCTGT 
ACCTACAATG AACCAGAGTC TTTGATCTAC AAAGATGCCC TTGTACTGCA TAAAGTCCTC 
CTTGAGACTC GGAGAGACCT GGAGGGAGAT GAGGATTCTC ATGTCCCTAA TGTGACGTTG 
CTGATTCAAG AGCTCATCCA TAACCTTTTT GTGTCAGTCA TGAGTCATCA GGATGACGAA 
GGGAGGTGTT ACAGCGACTC CTTAGCAGAA ATTCCTGCTG TGGATCCCAA CTCTCCCAAT 
AAACCTCCCC TTACATTTGA CATTATCAGG AAAAATGTTG AAAGTAATCG GTATCGGCGA 
CTTGATTTAT TTCAGGAGCA TATGTTTGAA GTATTGGAAC GGGCAAGAAG GATGAACCGG 
ACAGATTCCG AAATATATGA GGATGCTGTA GAACTTCAGC AGTTTTTTAT TAGAATTCGT 
GATGAACTCT GCAAAAATGG AGAGATCCTT CTTTCTCCAG CACTCAGCTA TACCACAAAA 
CACTTGCATA ACGATGTGGA AAAAGAAAAA AAGGAAAAAT TGCCTAAAGA AATAGAGGAA 
GATAAACTAA AACGCGAAGA AGAAAAAAGA GAAGCTGAAA AAAGTGAAGA TTCCTCAGGT 
ACTACAGGCC TCTCAGGCTT ACATCGTACA TACAGCCAGG ACTGCAGCTT TAAGAACAGC 
ATGTATCATG TCGGAGATTA TGTCTATGTT GAACCTGCGG AGGCCAATCT ACAACCACAT 
ATAGTGTGTA TTGAGAGACT GTGGGAGGAT TCAGCTGGTG AAAAATGGTT GTACGGCTGT 
TGGTTTTATC GGCCAAATGA AACATTCCAT TTGGCTACAC GAAAATTTCT AGAAAAAGAA 
GTTTTTAAGA GTGACTACTA CAATAAAGTA CCTGTTAGTA AAATTCTAGG CAAATGTGTA 
GTCATGTTTG TCAAGGAATA CTTTAAATTA TGTCCAGAAA ACTTTCGCGA TGAGGATGTT 
TTTGTCTGTG AATCGAGGTA TTCTGCCAAA ACCAAATCTT TTAAGAAAAT TAAACTGTGG 
ACCATGCCCA TCAGTTCAGT TAGATTTGTC CCTCGGGATG TGCCTTTGCC TGTGGTCCGA 
GTGGCCTCTG TGTTTGCAAA TGCAGATAAA GGGGATGATG AGAAGAATAC AGACAACTCA 
GATGACAATA GAGCTGAAGA CAATTTTAAC TTGGAAAAGG AAAAAGAAGA TGTTCCTGTG 
GAGATGTCCA ATGGTGAGCC AGGTTGCCAC TACTTTGAGC AGCTTCGGTA CAATGACATG 
TGGCTGAAGG TTGGTGATTG TGTCTTCATC AAATCCCACG GCTTGGTGCG CCCTCGTGTG 
GGCAGAATTG AGAAAGTATG GGTCCGAGAT GGAGCTGCAT ATTTTTATGG CCCTATCTTC 
ATTCATCCAG AAGAAACAGA ACATGAGCCC ACAAAAATGT TCTACAAAAA AGAAGTGTTT 
CTGAGTAATC TGGAAGAGAC CTGCCCTATG AGTTGTATTC TGGGGAAATG TGCAGTGCTG 
TCATTCAAGG ACTTCCTCTC CTGCAGGCCA ACTGAAATAC CAGAAAATGA CATTCTGCTT 
TGTGAGAGCC GCTATAATGA GAGTGACAAG CAGATGAAGA AGTTCAAGGG TTTGAAGAGG 
TTTTCACTCT CTGCTAAAGT TGTAGATGAT GAAATCTACT ACTTCAGAAA ACCAATCATT 
CCTCAGAAGG AACCCTCACC TTTGTTAGAA AAGAAGATAC AATTGCTAGA AGCTAAATTT 
GCAGAGTTAG AAGGAGGAGA TGATGATATT GAGGAGATGG GAGAAGAGGA TAGTGAAGTC 
ATTGAAGCTC CATCTCTACC TCAACTGCAG ACACCCCTGG CCAATGAGTT GGACCTCATG 
CCCTATACAC CCCCACAGCT TTCTGTCTTA CAGTCTACCC CAAAGTCTGC CAAAGGCAGT 
GCAAAGAAGG AAAGTTCTAA ACGAAAAATC AACATGAGTG GCTACATTTT GTTCAGCAGT 
GAAATGAGAG CTGTGATTAA AGCCCAGCAC CCAGACTACT CTTTTGGGGA GCTCAGCAGA 
CTGGTGGGGA CAGAATGGAG AAACCTTGAA ACAGCCAAGA AAGCAGAATA TGAAGAGCGG 
GCAGCTAAAG TTGCTGAGCA GCAGGAGAGA GAGCGAGCAG CACAGCAACA GCAGCCGAGT 
GCTTCTCCCC GAGCAGGCAC CCCTGTGGGG GCTCTCATGG GGGTGGTGCC ACCACCAACA 
CCAATGGGGA TGCTCAATCA GCAGTTGACA CCTGTTGCAG GCATGATGGG TGGCTATCCG 
CCAGGCCTTC CACCTTTGCA GGGCCCAGTT GATGGCCTTG TTAGCATGGG CAGCATGCAG 
CCACTTCACC CTGGGGGGCC TCCACCTCAC CATCTTCCGC CAGGTGTGCC TGGCCTCCCA 
GGCATCCCAC CACCGGGTGT GATGAATCAA GGAGTAGCCC CCATGGTAGG GACTCCAGCA 
CCAGGTGGAA GTCCGTATGG ACAACAGGTA GGAGTTTTGG GACCTCCAGG GCAGCAGGCA 
CCACCTCCAT ATCCTGGTCC TCATCCAGCT GGCCCCCCTG TCATACAGCA GCCAACAACG 
CCCATGTTTG TGGCTCCCCC ACCAAAGACC CAAAGGCTTC TCCACTCAGA GGCCTACCTG 
AAATACATTG AAGGACTCAG TGCTGAATCC AACAGCATTA GCAAGTGGGA CCAAACTTTG 
GCAGCTCGAA GACGGGATGT CCATTTGTCC AAAGAACAGG AGAGCCGCCT ACCTTCTCAC 
TGGCTCAAAA GTAAAGGGGC ACACACCACC ATGGCAGATG CCCTCTGGCG CCTACGGGAT 
TTAATGCTTC GAGACACTCT CAACATCCGA CAGGCATACA ACCTAGAAAA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519415.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519478.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X23, mRNA.

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CloneID	OMu50261
Clone ID Related Accession (Same CDS sequence)	XM_017316147.1 , XM_006519415.3
Accession Version	XM_006519415.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519415.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGGA ATACTCATGA CAGTGAGATG TTGGGTCTCA GGAGGCTATC CAGTAAAAAG 
AACATAAGAA AACAGCGAAT GAAAATTTTA TTCAATGTTG TTCTTGAAGC TCGAGAGCCA 
GGTTCAGGCA GAAGACTTTG CGATCTATTT ATGGTTAAGC CATCCAAGAA GGACTATCCT 
GATTATTATA AAATCATCTT AGAGCCAATG GACCTGAAAA TAATTGAGCA TAACATCCGA 
AATGACAAAT ATGCAGGTGA AGAAGGAATG ATGGAAGACA TGAAACTCAT GTTCCGCAAT 
GCCAGGCACT ACAATGAGGA GGGCTCCCAG GTATACAATG ATGCCCATAT CCTGGAGAAG 
TTACTCAAAG ATAAAAGGAA AGAGCTGGGC CCTCTGCCTG ATGATGATGA CATGGCTTCT 
CCCAAACTTA AATTGAGTAG GAAGAGTGGT GTTTCTCCTA AGAAATCAAA GTACATGACT 
CCAATGCAGC AGAAACTGAA TGAAGTGTAT GAAGCTGTAA AGAACTATAC TGATAAGAGG 
GGTCGCCGCC TTAGTGCTAT ATTTCTAAGA CTCCCCTCTA GATCAGAGCT GCCTGACTAC 
TACCTGACCA TTAAAAAGCC CATGGACATG GAAAAAATTC GAAGTCACAT GATGGCAAAC 
AAGTACCAAG ACATAGATTC TATGGTAGAG GACTTTGTCA TGATGTTTAA TAATGCCTGT 
ACCTACAATG AACCAGAGTC TTTGATCTAC AAAGATGCCC TTGTACTGCA TAAAGTCCTC 
CTTGAGACTC GGAGAGACCT GGAGGGAGAT GAGGATTCTC ATGTCCCTAA TGTGACGTTG 
CTGATTCAAG AGCTCATCCA TAACCTTTTT GTGTCAGTCA TGAGTCATCA GGATGACGAA 
GGGAGGTGTT ACAGCGACTC CTTAGCAGAA ATTCCTGCTG TGGATCCCAA CTCTCCCAAT 
AAACCTCCCC TTACATTTGA CATTATCAGG AAAAATGTTG AAAGTAATCG GTATCGGCGA 
CTTGATTTAT TTCAGGAGCA TATGTTTGAA GTATTGGAAC GGGCAAGAAG GATGAACCGG 
ACAGATTCCG AAATATATGA GGATGCTGTA GAACTTCAGC AGTTTTTTAT TAGAATTCGT 
GATGAACTCT GCAAAAATGG AGAGATCCTT CTTTCTCCAG CACTCAGCTA TACCACAAAA 
CACTTGCATA ACGATGTGGA AAAAGAAAAA AAGGAAAAAT TGCCTAAAGA AATAGAGGAA 
GATAAACTAA AACGCGAAGA AGAAAAAAGA GAAGCTGAAA AAAGTGAAGA TTCCTCAGGT 
ACTACAGGCC TCTCAGGCTT ACATCGTACA TACAGCCAGG ACTGCAGCTT TAAGAACAGC 
ATGTATCATG TCGGAGATTA TGTCTATGTT GAACCTGCGG AGGCCAATCT ACAACCACAT 
ATAGTGTGTA TTGAGAGACT GTGGGAGGAT TCAGCTGGTG AAAAATGGTT GTACGGCTGT 
TGGTTTTATC GGCCAAATGA AACATTCCAT TTGGCTACAC GAAAATTTCT AGAAAAAGAA 
GTTTTTAAGA GTGACTACTA CAATAAAGTA CCTGTTAGTA AAATTCTAGG CAAATGTGTA 
GTCATGTTTG TCAAGGAATA CTTTAAATTA TGTCCAGAAA ACTTTCGCGA TGAGGATGTT 
TTTGTCTGTG AATCGAGGTA TTCTGCCAAA ACCAAATCTT TTAAGAAAAT TAAACTGTGG 
ACCATGCCCA TCAGTTCAGT TAGATTTGTC CCTCGGGATG TGCCTTTGCC TGTGGTCCGA 
GTGGCCTCTG TGTTTGCAAA TGCAGATAAA GGGGATGATG AGAAGAATAC AGACAACTCA 
GATGACAATA GAGCTGAAGA CAATTTTAAC TTGGAAAAGG AAAAAGAAGA TGTTCCTGTG 
GAGATGTCCA ATGGTGAGCC AGGTTGCCAC TACTTTGAGC AGCTTCGGTA CAATGACATG 
TGGCTGAAGG TTGGTGATTG TGTCTTCATC AAATCCCACG GCTTGGTGCG CCCTCGTGTG 
GGCAGAATTG AGAAAGTATG GGTCCGAGAT GGAGCTGCAT ATTTTTATGG CCCTATCTTC 
ATTCATCCAG AAGAAACAGA ACATGAGCCC ACAAAAATGT TCTACAAAAA AGAAGTGTTT 
CTGAGTAATC TGGAAGAGAC CTGCCCTATG AGTTGTATTC TGGGGAAATG TGCAGTGCTG 
TCATTCAAGG ACTTCCTCTC CTGCAGGCCA ACTGAAATAC CAGAAAATGA CATTCTGCTT 
TGTGAGAGCC GCTATAATGA GAGTGACAAG CAGATGAAGA AGTTCAAGGG TTTGAAGAGG 
TTTTCACTCT CTGCTAAAGT TGTAGATGAT GAAATCTACT ACTTCAGAAA ACCAATCATT 
CCTCAGAAGG AACCCTCACC TTTGTTAGAA AAGAAGATAC AATTGCTAGA AGCTAAATTT 
GCAGAGTTAG AAGGAGGAGA TGATGATATT GAGGAGATGG GAGAAGAGGA TAGTGAAGTC 
ATTGAAGCTC CATCTCTACC TCAACTGCAG ACACCCCTGG CCAATGAGTT GGACCTCATG 
CCCTATACAC CCCCACAGCT TTCTGTCTTA CAGTCTACCC CAAAGTCTGC CAAAGGCAGT 
GCAAAGAAGG AAAGTTCTAA ACGAAAAATC AACATGAGTG GCTACATTTT GTTCAGCAGT 
GAAATGAGAG CTGTGATTAA AGCCCAGCAC CCAGACTACT CTTTTGGGGA GCTCAGCAGA 
CTGGTGGGGA CAGAATGGAG AAACCTTGAA ACAGCCAAGA AAGCAGAATA TGAAGAGCGG 
GCAGCTAAAG TTGCTGAGCA GCAGGAGAGA GAGCGAGCAG CACAGCAACA GCAGCCGAGT 
GCTTCTCCCC GAGCAGGCAC CCCTGTGGGG GCTCTCATGG GGGTGGTGCC ACCACCAACA 
CCAATGGGGA TGCTCAATCA GCAGTTGACA CCTGTTGCAG GCATGATGGG TGGCTATCCG 
CCAGGCCTTC CACCTTTGCA GGGCCCAGTT GATGGCCTTG TTAGCATGGG CAGCATGCAG 
CCACTTCACC CTGGGGGGCC TCCACCTCAC CATCTTCCGC CAGGTGTGCC TGGCCTCCCA 
GGCATCCCAC CACCGGGTGT GATGAATCAA GGAGTAGCCC CCATGGTAGG GACTCCAGCA 
CCAGGTGGAA GTCCGTATGG ACAACAGGTA GGAGTTTTGG GACCTCCAGG GCAGCAGGCA 
CCACCTCCAT ATCCTGGTCC TCATCCAGCT GGCCCCCCTG TCATACAGCA GCCAACAACG 
CCCATGTTTG TGGCTCCCCC ACCAAAGACC CAAAGGCTTC TCCACTCAGA GGCCTACCTG 
AAATACATTG AAGGACTCAG TGCTGAATCC AACAGCATTA GCAAGTGGGA CCAAACTTTG 
GCAGCTCGAA GACGGGATGT CCATTTGTCC AAAGAACAGG AGAGCCGCCT ACCTTCTCAC 
TGGCTCAAAA GTAAAGGGGC ACACACCACC ATGGCAGATG CCCTCTGGCG CCTACGGGAT 
TTAATGCTTC GAGACACTCT CAACATCCGA CAGGCATACA ACCTAGAAAA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519478.1
CDS	359..5455
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519415.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519415.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGGA ATACTCATGA CAGTGAGATG TTGGGTCTCA GGAGGCTATC CAGTAAAAAG 
AACATAAGAA AACAGCGAAT GAAAATTTTA TTCAATGTTG TTCTTGAAGC TCGAGAGCCA 
GGTTCAGGCA GAAGACTTTG CGATCTATTT ATGGTTAAGC CATCCAAGAA GGACTATCCT 
GATTATTATA AAATCATCTT AGAGCCAATG GACCTGAAAA TAATTGAGCA TAACATCCGA 
AATGACAAAT ATGCAGGTGA AGAAGGAATG ATGGAAGACA TGAAACTCAT GTTCCGCAAT 
GCCAGGCACT ACAATGAGGA GGGCTCCCAG GTATACAATG ATGCCCATAT CCTGGAGAAG 
TTACTCAAAG ATAAAAGGAA AGAGCTGGGC CCTCTGCCTG ATGATGATGA CATGGCTTCT 
CCCAAACTTA AATTGAGTAG GAAGAGTGGT GTTTCTCCTA AGAAATCAAA GTACATGACT 
CCAATGCAGC AGAAACTGAA TGAAGTGTAT GAAGCTGTAA AGAACTATAC TGATAAGAGG 
GGTCGCCGCC TTAGTGCTAT ATTTCTAAGA CTCCCCTCTA GATCAGAGCT GCCTGACTAC 
TACCTGACCA TTAAAAAGCC CATGGACATG GAAAAAATTC GAAGTCACAT GATGGCAAAC 
AAGTACCAAG ACATAGATTC TATGGTAGAG GACTTTGTCA TGATGTTTAA TAATGCCTGT 
ACCTACAATG AACCAGAGTC TTTGATCTAC AAAGATGCCC TTGTACTGCA TAAAGTCCTC 
CTTGAGACTC GGAGAGACCT GGAGGGAGAT GAGGATTCTC ATGTCCCTAA TGTGACGTTG 
CTGATTCAAG AGCTCATCCA TAACCTTTTT GTGTCAGTCA TGAGTCATCA GGATGACGAA 
GGGAGGTGTT ACAGCGACTC CTTAGCAGAA ATTCCTGCTG TGGATCCCAA CTCTCCCAAT 
AAACCTCCCC TTACATTTGA CATTATCAGG AAAAATGTTG AAAGTAATCG GTATCGGCGA 
CTTGATTTAT TTCAGGAGCA TATGTTTGAA GTATTGGAAC GGGCAAGAAG GATGAACCGG 
ACAGATTCCG AAATATATGA GGATGCTGTA GAACTTCAGC AGTTTTTTAT TAGAATTCGT 
GATGAACTCT GCAAAAATGG AGAGATCCTT CTTTCTCCAG CACTCAGCTA TACCACAAAA 
CACTTGCATA ACGATGTGGA AAAAGAAAAA AAGGAAAAAT TGCCTAAAGA AATAGAGGAA 
GATAAACTAA AACGCGAAGA AGAAAAAAGA GAAGCTGAAA AAAGTGAAGA TTCCTCAGGT 
ACTACAGGCC TCTCAGGCTT ACATCGTACA TACAGCCAGG ACTGCAGCTT TAAGAACAGC 
ATGTATCATG TCGGAGATTA TGTCTATGTT GAACCTGCGG AGGCCAATCT ACAACCACAT 
ATAGTGTGTA TTGAGAGACT GTGGGAGGAT TCAGCTGGTG AAAAATGGTT GTACGGCTGT 
TGGTTTTATC GGCCAAATGA AACATTCCAT TTGGCTACAC GAAAATTTCT AGAAAAAGAA 
GTTTTTAAGA GTGACTACTA CAATAAAGTA CCTGTTAGTA AAATTCTAGG CAAATGTGTA 
GTCATGTTTG TCAAGGAATA CTTTAAATTA TGTCCAGAAA ACTTTCGCGA TGAGGATGTT 
TTTGTCTGTG AATCGAGGTA TTCTGCCAAA ACCAAATCTT TTAAGAAAAT TAAACTGTGG 
ACCATGCCCA TCAGTTCAGT TAGATTTGTC CCTCGGGATG TGCCTTTGCC TGTGGTCCGA 
GTGGCCTCTG TGTTTGCAAA TGCAGATAAA GGGGATGATG AGAAGAATAC AGACAACTCA 
GATGACAATA GAGCTGAAGA CAATTTTAAC TTGGAAAAGG AAAAAGAAGA TGTTCCTGTG 
GAGATGTCCA ATGGTGAGCC AGGTTGCCAC TACTTTGAGC AGCTTCGGTA CAATGACATG 
TGGCTGAAGG TTGGTGATTG TGTCTTCATC AAATCCCACG GCTTGGTGCG CCCTCGTGTG 
GGCAGAATTG AGAAAGTATG GGTCCGAGAT GGAGCTGCAT ATTTTTATGG CCCTATCTTC 
ATTCATCCAG AAGAAACAGA ACATGAGCCC ACAAAAATGT TCTACAAAAA AGAAGTGTTT 
CTGAGTAATC TGGAAGAGAC CTGCCCTATG AGTTGTATTC TGGGGAAATG TGCAGTGCTG 
TCATTCAAGG ACTTCCTCTC CTGCAGGCCA ACTGAAATAC CAGAAAATGA CATTCTGCTT 
TGTGAGAGCC GCTATAATGA GAGTGACAAG CAGATGAAGA AGTTCAAGGG TTTGAAGAGG 
TTTTCACTCT CTGCTAAAGT TGTAGATGAT GAAATCTACT ACTTCAGAAA ACCAATCATT 
CCTCAGAAGG AACCCTCACC TTTGTTAGAA AAGAAGATAC AATTGCTAGA AGCTAAATTT 
GCAGAGTTAG AAGGAGGAGA TGATGATATT GAGGAGATGG GAGAAGAGGA TAGTGAAGTC 
ATTGAAGCTC CATCTCTACC TCAACTGCAG ACACCCCTGG CCAATGAGTT GGACCTCATG 
CCCTATACAC CCCCACAGCT TTCTGTCTTA CAGTCTACCC CAAAGTCTGC CAAAGGCAGT 
GCAAAGAAGG AAAGTTCTAA ACGAAAAATC AACATGAGTG GCTACATTTT GTTCAGCAGT 
GAAATGAGAG CTGTGATTAA AGCCCAGCAC CCAGACTACT CTTTTGGGGA GCTCAGCAGA 
CTGGTGGGGA CAGAATGGAG AAACCTTGAA ACAGCCAAGA AAGCAGAATA TGAAGAGCGG 
GCAGCTAAAG TTGCTGAGCA GCAGGAGAGA GAGCGAGCAG CACAGCAACA GCAGCCGAGT 
GCTTCTCCCC GAGCAGGCAC CCCTGTGGGG GCTCTCATGG GGGTGGTGCC ACCACCAACA 
CCAATGGGGA TGCTCAATCA GCAGTTGACA CCTGTTGCAG GCATGATGGG TGGCTATCCG 
CCAGGCCTTC CACCTTTGCA GGGCCCAGTT GATGGCCTTG TTAGCATGGG CAGCATGCAG 
CCACTTCACC CTGGGGGGCC TCCACCTCAC CATCTTCCGC CAGGTGTGCC TGGCCTCCCA 
GGCATCCCAC CACCGGGTGT GATGAATCAA GGAGTAGCCC CCATGGTAGG GACTCCAGCA 
CCAGGTGGAA GTCCGTATGG ACAACAGGTA GGAGTTTTGG GACCTCCAGG GCAGCAGGCA 
CCACCTCCAT ATCCTGGTCC TCATCCAGCT GGCCCCCCTG TCATACAGCA GCCAACAACG 
CCCATGTTTG TGGCTCCCCC ACCAAAGACC CAAAGGCTTC TCCACTCAGA GGCCTACCTG 
AAATACATTG AAGGACTCAG TGCTGAATCC AACAGCATTA GCAAGTGGGA CCAAACTTTG 
GCAGCTCGAA GACGGGATGT CCATTTGTCC AAAGAACAGG AGAGCCGCCT ACCTTCTCAC 
TGGCTCAAAA GTAAAGGGGC ACACACCACC ATGGCAGATG CCCTCTGGCG CCTACGGGAT 
TTAATGCTTC GAGACACTCT CAACATCCGA CAGGCATACA ACCTAGAAAA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519416.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519479.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OMu50262
Clone ID Related Accession (Same CDS sequence)	XM_006519416.1
Accession Version	XM_006519416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GTAAAAAGAA CATAAGAAAA 
CAGCGAATGA AAATTTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGCG ATCTATTTAT GGTTAAGCCA TCCAAGAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTAG AGCCAATGGA CCTGAAAATA ATTGAGCATA ACATCCGAAA TGACAAATAT 
GCAGGTGAAG AAGGAATGAT GGAAGACATG AAACTCATGT TCCGCAATGC CAGGCACTAC 
AATGAGGAGG GCTCCCAGGT ATACAATGAT GCCCATATCC TGGAGAAGTT ACTCAAAGAT 
AAAAGGAAAG AGCTGGGCCC TCTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTTAAA 
TTGAGTAGGA AGAGTGGTGT TTCTCCTAAG AAATCAAAGT ACATGACTCC AATGCAGCAG 
AAACTGAATG AAGTGTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTT 
AGTGCTATAT TTCTAAGACT CCCCTCTAGA TCAGAGCTGC CTGACTACTA CCTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCAAACAA GTACCAAGAC 
ATAGATTCTA TGGTAGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC CTACAATGAA 
CCAGAGTCTT TGATCTACAA AGATGCCCTT GTACTGCATA AAGTCCTCCT TGAGACTCGG 
AGAGACCTGG AGGGAGATGA GGATTCTCAT GTCCCTAATG TGACGTTGCT GATTCAAGAG 
CTCATCCATA ACCTTTTTGT GTCAGTCATG AGTCATCAGG ATGACGAAGG GAGGTGTTAC 
AGCGACTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAACT CTCCCAATAA ACCTCCCCTT 
ACATTTGACA TTATCAGGAA AAATGTTGAA AGTAATCGGT ATCGGCGACT TGATTTATTT 
CAGGAGCATA TGTTTGAAGT ATTGGAACGG GCAAGAAGGA TGAACCGGAC AGATTCCGAA 
ATATATGAGG ATGCTGTAGA ACTTCAGCAG TTTTTTATTA GAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCTCCAGCA CTCAGCTATA CCACAAAACA CTTGCATAAC 
GATGTGGAAA AAGAAAAAAA GGAAAAATTG CCTAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGCGAAGAAG AAAAAAGAGA AGCTGAAAAA AGTGAAGATT CCTCAGGTAC TACAGGCCTC 
TCAGGCTTAC ATCGTACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTATCATGTC 
GGAGATTATG TCTATGTTGA ACCTGCGGAG GCCAATCTAC AACCACATAT AGTGTGTATT 
GAGAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ACGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTACTACA ATAAAGTACC TGTTAGTAAA ATTCTAGGCA AATGTGTAGT CATGTTTGTC 
AAGGAATACT TTAAATTATG TCCAGAAAAC TTTCGCGATG AGGATGTTTT TGTCTGTGAA 
TCGAGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGTTCAGTTA GATTTGTCCC TCGGGATGTG CCTTTGCCTG TGGTCCGAGT GGCCTCTGTG 
TTTGCAAATG CAGATAAAGG GGATGATGAG AAGAATACAG ACAACTCAGA TGACAATAGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TTCCTGTGGA GATGTCCAAT 
GGTGAGCCAG GTTGCCACTA CTTTGAGCAG CTTCGGTACA ATGACATGTG GCTGAAGGTT 
GGTGATTGTG TCTTCATCAA ATCCCACGGC TTGGTGCGCC CTCGTGTGGG CAGAATTGAG 
AAAGTATGGG TCCGAGATGG AGCTGCATAT TTTTATGGCC CTATCTTCAT TCATCCAGAA 
GAAACAGAAC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTGTTTCT GAGTAATCTG 
GAAGAGACCT GCCCTATGAG TTGTATTCTG GGGAAATGTG CAGTGCTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TATAATGAGA GTGACAAGCA GATGAAGAAG TTCAAGGGTT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTTG TAGATGATGA AATCTACTAC TTCAGAAAAC CAATCATTCC TCAGAAGGAA 
CCCTCACCTT TGTTAGAAAA GAAGATACAA TTGCTAGAAG CTAAATTTGC AGAGTTAGAA 
GGAGGAGATG ATGATATTGA GGAGATGGGA GAAGAGGATA GTGAAGTCAT TGAAGCTCCA 
TCTCTACCTC AACTGCAGAC ACCCCTGGCC AATGAGTTGG ACCTCATGCC CTATACACCC 
CCACAGCTTT CTGTCTTACA GTCTACCCCA AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA 
AGTTCTAAAC GAAAAATCAA CATGAGTGGC TACATTTTGT TCAGCAGTGA AATGAGAGCT 
GTGATTAAAG CCCAGCACCC AGACTACTCT TTTGGGGAGC TCAGCAGACT GGTGGGGACA 
GAATGGAGAA ACCTTGAAAC AGCCAAGAAA GCAGAATATG AAGAGCGGGC AGCTAAAGTT 
GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA CAGCAACAGC AGCCGAGTGC TTCTCCCCGA 
GCAGGCACCC CTGTGGGGGC TCTCATGGGG GTGGTGCCAC CACCAACACC AATGGGGATG 
CTCAATCAGC AGTTGACACC TGTTGCAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCTCACCA TCTTCCGCCA GGTGTGCCTG GCCTCCCAGG CATCCCACCA 
CCGGGTGTGA TGAATCAAGG AGTAGCCCCC ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AACAGGTAGG AGTTTTGGGA CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGTCCTC ATCCAGCTGG CCCCCCTGTC ATACAGCAGC CAACAACGCC CATGTTTGTG 
GCTCCCCCAC CAAAGACCCA AAGGCTTCTC CACTCAGAGG CCTACCTGAA ATACATTGAA 
GGACTCAGTG CTGAATCCAA CAGCATTAGC AAGTGGGACC AAACTTTGGC AGCTCGAAGA 
CGGGATGTCC ATTTGTCCAA AGAACAGGAG AGCCGCCTAC CTTCTCACTG GCTCAAAAGT 
AAAGGGGCAC ACACCACCAT GGCAGATGCC CTCTGGCGCC TACGGGATTT AATGCTTCGA 
GACACTCTCA ACATCCGACA GGCATACAAC CTAGAAAATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519479.1
CDS	312..5396
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519416.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519416.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGG 
ACTGCATCAA ATTTGGCTGC AGCCAGGCTG ACAGGTCCTT CGCACAATAA AAGCAGCCTT 
GGTGAAGAAA GAAACCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGCTTGTCAG CAATTACCAT GGCACTTCAG TATGGATCAG AGAGTGAAGA GGACGCTGCT 
TTAGCTGCTG CACGCTATGA AGAAGGGGAA TCTGAAGCAG AGAGCATCAC TTCCTTCATG 
GACGTTTCCA ACCCCTTTCA TCAGCTTTAC GACACAGTTA GGAGCTGTAG GAATCACCAA 
GGGCAGCTCA TAGCTGAACC TTTCTTCCAT TTGCCTTCAA AGAAAAAATA CCCAGATTAT 
TATCAGCAAA TTAAAATGCC CATATCACTT CAACAGATCA GAACAAAGCT AAAGAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGTTATAACG TTCCCAATTC AGCCATCTAT AAGCGAGTTC TAAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAGGAGCT TGCGAGGAGA GATGACATTG AGGACGGAGA CAGCATGATC 
TCCTCAGCCA CTTCTGACAC TGGTAGTGCC AAAAGGAAAA GTAAAAAGAA CATAAGAAAA 
CAGCGAATGA AAATTTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGCG ATCTATTTAT GGTTAAGCCA TCCAAGAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTAG AGCCAATGGA CCTGAAAATA ATTGAGCATA ACATCCGAAA TGACAAATAT 
GCAGGTGAAG AAGGAATGAT GGAAGACATG AAACTCATGT TCCGCAATGC CAGGCACTAC 
AATGAGGAGG GCTCCCAGGT ATACAATGAT GCCCATATCC TGGAGAAGTT ACTCAAAGAT 
AAAAGGAAAG AGCTGGGCCC TCTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTTAAA 
TTGAGTAGGA AGAGTGGTGT TTCTCCTAAG AAATCAAAGT ACATGACTCC AATGCAGCAG 
AAACTGAATG AAGTGTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTT 
AGTGCTATAT TTCTAAGACT CCCCTCTAGA TCAGAGCTGC CTGACTACTA CCTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCAAACAA GTACCAAGAC 
ATAGATTCTA TGGTAGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC CTACAATGAA 
CCAGAGTCTT TGATCTACAA AGATGCCCTT GTACTGCATA AAGTCCTCCT TGAGACTCGG 
AGAGACCTGG AGGGAGATGA GGATTCTCAT GTCCCTAATG TGACGTTGCT GATTCAAGAG 
CTCATCCATA ACCTTTTTGT GTCAGTCATG AGTCATCAGG ATGACGAAGG GAGGTGTTAC 
AGCGACTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAACT CTCCCAATAA ACCTCCCCTT 
ACATTTGACA TTATCAGGAA AAATGTTGAA AGTAATCGGT ATCGGCGACT TGATTTATTT 
CAGGAGCATA TGTTTGAAGT ATTGGAACGG GCAAGAAGGA TGAACCGGAC AGATTCCGAA 
ATATATGAGG ATGCTGTAGA ACTTCAGCAG TTTTTTATTA GAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCTCCAGCA CTCAGCTATA CCACAAAACA CTTGCATAAC 
GATGTGGAAA AAGAAAAAAA GGAAAAATTG CCTAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGCGAAGAAG AAAAAAGAGA AGCTGAAAAA AGTGAAGATT CCTCAGGTAC TACAGGCCTC 
TCAGGCTTAC ATCGTACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTATCATGTC 
GGAGATTATG TCTATGTTGA ACCTGCGGAG GCCAATCTAC AACCACATAT AGTGTGTATT 
GAGAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ACGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTACTACA ATAAAGTACC TGTTAGTAAA ATTCTAGGCA AATGTGTAGT CATGTTTGTC 
AAGGAATACT TTAAATTATG TCCAGAAAAC TTTCGCGATG AGGATGTTTT TGTCTGTGAA 
TCGAGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGTTCAGTTA GATTTGTCCC TCGGGATGTG CCTTTGCCTG TGGTCCGAGT GGCCTCTGTG 
TTTGCAAATG CAGATAAAGG GGATGATGAG AAGAATACAG ACAACTCAGA TGACAATAGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TTCCTGTGGA GATGTCCAAT 
GGTGAGCCAG GTTGCCACTA CTTTGAGCAG CTTCGGTACA ATGACATGTG GCTGAAGGTT 
GGTGATTGTG TCTTCATCAA ATCCCACGGC TTGGTGCGCC CTCGTGTGGG CAGAATTGAG 
AAAGTATGGG TCCGAGATGG AGCTGCATAT TTTTATGGCC CTATCTTCAT TCATCCAGAA 
GAAACAGAAC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTGTTTCT GAGTAATCTG 
GAAGAGACCT GCCCTATGAG TTGTATTCTG GGGAAATGTG CAGTGCTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TATAATGAGA GTGACAAGCA GATGAAGAAG TTCAAGGGTT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTTG TAGATGATGA AATCTACTAC TTCAGAAAAC CAATCATTCC TCAGAAGGAA 
CCCTCACCTT TGTTAGAAAA GAAGATACAA TTGCTAGAAG CTAAATTTGC AGAGTTAGAA 
GGAGGAGATG ATGATATTGA GGAGATGGGA GAAGAGGATA GTGAAGTCAT TGAAGCTCCA 
TCTCTACCTC AACTGCAGAC ACCCCTGGCC AATGAGTTGG ACCTCATGCC CTATACACCC 
CCACAGCTTT CTGTCTTACA GTCTACCCCA AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA 
AGTTCTAAAC GAAAAATCAA CATGAGTGGC TACATTTTGT TCAGCAGTGA AATGAGAGCT 
GTGATTAAAG CCCAGCACCC AGACTACTCT TTTGGGGAGC TCAGCAGACT GGTGGGGACA 
GAATGGAGAA ACCTTGAAAC AGCCAAGAAA GCAGAATATG AAGAGCGGGC AGCTAAAGTT 
GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA CAGCAACAGC AGCCGAGTGC TTCTCCCCGA 
GCAGGCACCC CTGTGGGGGC TCTCATGGGG GTGGTGCCAC CACCAACACC AATGGGGATG 
CTCAATCAGC AGTTGACACC TGTTGCAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCTCACCA TCTTCCGCCA GGTGTGCCTG GCCTCCCAGG CATCCCACCA 
CCGGGTGTGA TGAATCAAGG AGTAGCCCCC ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AACAGGTAGG AGTTTTGGGA CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGTCCTC ATCCAGCTGG CCCCCCTGTC ATACAGCAGC CAACAACGCC CATGTTTGTG 
GCTCCCCCAC CAAAGACCCA AAGGCTTCTC CACTCAGAGG CCTACCTGAA ATACATTGAA 
GGACTCAGTG CTGAATCCAA CAGCATTAGC AAGTGGGACC AAACTTTGGC AGCTCGAAGA 
CGGGATGTCC ATTTGTCCAA AGAACAGGAG AGCCGCCTAC CTTCTCACTG GCTCAAAAGT 
AAAGGGGCAC ACACCACCAT GGCAGATGCC CTCTGGCGCC TACGGGATTT AATGCTTCGA 
GACACTCTCA ACATCCGACA GGCATACAAC CTAGAAAATG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519417.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519480.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OMu50263
Clone ID Related Accession (Same CDS sequence)	XM_006519417.1
Accession Version	XM_006519417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGTA AAAAGAACAT AAGAAAACAG CGAATGAAAA TTTTATTCAA TGTTGTTCTT 
GAAGCTCGAG AGCCAGGTTC AGGCAGAAGA CTTTGCGATC TATTTATGGT TAAGCCATCC 
AAGAAGGACT ATCCTGATTA TTATAAAATC ATCTTAGAGC CAATGGACCT GAAAATAATT 
GAGCATAACA TCCGAAATGA CAAATATGCA GGTGAAGAAG GAATGATGGA AGACATGAAA 
CTCATGTTCC GCAATGCCAG GCACTACAAT GAGGAGGGCT CCCAGGTATA CAATGATGCC 
CATATCCTGG AGAAGTTACT CAAAGATAAA AGGAAAGAGC TGGGCCCTCT GCCTGATGAT 
GATGACATGG CTTCTCCCAA ACTTAAATTG AGTAGGAAGA GTGGTGTTTC TCCTAAGAAA 
TCAAAGTACA TGACTCCAAT GCAGCAGAAA CTGAATGAAG TGTATGAAGC TGTAAAGAAC 
TATACTGATA AGAGGGGTCG CCGCCTTAGT GCTATATTTC TAAGACTCCC CTCTAGATCA 
GAGCTGCCTG ACTACTACCT GACCATTAAA AAGCCCATGG ACATGGAAAA AATTCGAAGT 
CACATGATGG CAAACAAGTA CCAAGACATA GATTCTATGG TAGAGGACTT TGTCATGATG 
TTTAATAATG CCTGTACCTA CAATGAACCA GAGTCTTTGA TCTACAAAGA TGCCCTTGTA 
CTGCATAAAG TCCTCCTTGA GACTCGGAGA GACCTGGAGG GAGATGAGGA TTCTCATGTC 
CCTAATGTGA CGTTGCTGAT TCAAGAGCTC ATCCATAACC TTTTTGTGTC AGTCATGAGT 
CATCAGGATG ACGAAGGGAG GTGTTACAGC GACTCCTTAG CAGAAATTCC TGCTGTGGAT 
CCCAACTCTC CCAATAAACC TCCCCTTACA TTTGACATTA TCAGGAAAAA TGTTGAAAGT 
AATCGGTATC GGCGACTTGA TTTATTTCAG GAGCATATGT TTGAAGTATT GGAACGGGCA 
AGAAGGATGA ACCGGACAGA TTCCGAAATA TATGAGGATG CTGTAGAACT TCAGCAGTTT 
TTTATTAGAA TTCGTGATGA ACTCTGCAAA AATGGAGAGA TCCTTCTTTC TCCAGCACTC 
AGCTATACCA CAAAACACTT GCATAACGAT GTGGAAAAAG AAAAAAAGGA AAAATTGCCT 
AAAGAAATAG AGGAAGATAA ACTAAAACGC GAAGAAGAAA AAAGAGAAGC TGAAAAAAGT 
GAAGATTCCT CAGGTACTAC AGGCCTCTCA GGCTTACATC GTACATACAG CCAGGACTGC 
AGCTTTAAGA ACAGCATGTA TCATGTCGGA GATTATGTCT ATGTTGAACC TGCGGAGGCC 
AATCTACAAC CACATATAGT GTGTATTGAG AGACTGTGGG AGGATTCAGC TGGTGAAAAA 
TGGTTGTACG GCTGTTGGTT TTATCGGCCA AATGAAACAT TCCATTTGGC TACACGAAAA 
TTTCTAGAAA AAGAAGTTTT TAAGAGTGAC TACTACAATA AAGTACCTGT TAGTAAAATT 
CTAGGCAAAT GTGTAGTCAT GTTTGTCAAG GAATACTTTA AATTATGTCC AGAAAACTTT 
CGCGATGAGG ATGTTTTTGT CTGTGAATCG AGGTATTCTG CCAAAACCAA ATCTTTTAAG 
AAAATTAAAC TGTGGACCAT GCCCATCAGT TCAGTTAGAT TTGTCCCTCG GGATGTGCCT 
TTGCCTGTGG TCCGAGTGGC CTCTGTGTTT GCAAATGCAG ATAAAGGGGA TGATGAGAAG 
AATACAGACA ACTCAGATGA CAATAGAGCT GAAGACAATT TTAACTTGGA AAAGGAAAAA 
GAAGATGTTC CTGTGGAGAT GTCCAATGGT GAGCCAGGTT GCCACTACTT TGAGCAGCTT 
CGGTACAATG ACATGTGGCT GAAGGTTGGT GATTGTGTCT TCATCAAATC CCACGGCTTG 
GTGCGCCCTC GTGTGGGCAG AATTGAGAAA GTATGGGTCC GAGATGGAGC TGCATATTTT 
TATGGCCCTA TCTTCATTCA TCCAGAAGAA ACAGAACATG AGCCCACAAA AATGTTCTAC 
AAAAAAGAAG TGTTTCTGAG TAATCTGGAA GAGACCTGCC CTATGAGTTG TATTCTGGGG 
AAATGTGCAG TGCTGTCATT CAAGGACTTC CTCTCCTGCA GGCCAACTGA AATACCAGAA 
AATGACATTC TGCTTTGTGA GAGCCGCTAT AATGAGAGTG ACAAGCAGAT GAAGAAGTTC 
AAGGGTTTGA AGAGGTTTTC ACTCTCTGCT AAAGTTGTAG ATGATGAAAT CTACTACTTC 
AGAAAACCAA TCATTCCTCA GAAGGAACCC TCACCTTTGT TAGAAAAGAA GATACAATTG 
CTAGAAGCTA AATTTGCAGA GTTAGAAGGA GGAGATGATG ATATTGAGGA GATGGGAGAA 
GAGGATAGTG AAGTCATTGA AGCTCCATCT CTACCTCAAC TGCAGACACC CCTGGCCAAT 
GAGTTGGACC TCATGCCCTA TACACCCCCA CAGCTTTCTG TCTTACAGTC TACCCCAAAG 
TCTGCCAAAG GCAGTGCAAA GAAGGAAAGT TCTAAACGAA AAATCAACAT GAGTGGCTAC 
ATTTTGTTCA GCAGTGAAAT GAGAGCTGTG ATTAAAGCCC AGCACCCAGA CTACTCTTTT 
GGGGAGCTCA GCAGACTGGT GGGGACAGAA TGGAGAAACC TTGAAACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CTCACCATCT TCCGCCAGGT 
GTGCCTGGCC TCCCAGGCAT CCCACCACCG GGTGTGATGA ATCAAGGAGT AGCCCCCATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAAC AGGTAGGAGT TTTGGGACCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGTCCTCATC CAGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACGCCCAT GTTTGTGGCT CCCCCACCAA AGACCCAAAG GCTTCTCCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCTG AATCCAACAG CATTAGCAAG 
TGGGACCAAA CTTTGGCAGC TCGAAGACGG GATGTCCATT TGTCCAAAGA ACAGGAGAGC 
CGCCTACCTT CTCACTGGCT CAAAAGTAAA GGGGCACACA CCACCATGGC AGATGCCCTC 
TGGCGCCTAC GGGATTTAAT GCTTCGAGAC ACTCTCAACA TCCGACAGGC ATACAACCTA 
GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519480.1
CDS	343..5394
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519417.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519417.1

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGTA AAAAGAACAT AAGAAAACAG CGAATGAAAA TTTTATTCAA TGTTGTTCTT 
GAAGCTCGAG AGCCAGGTTC AGGCAGAAGA CTTTGCGATC TATTTATGGT TAAGCCATCC 
AAGAAGGACT ATCCTGATTA TTATAAAATC ATCTTAGAGC CAATGGACCT GAAAATAATT 
GAGCATAACA TCCGAAATGA CAAATATGCA GGTGAAGAAG GAATGATGGA AGACATGAAA 
CTCATGTTCC GCAATGCCAG GCACTACAAT GAGGAGGGCT CCCAGGTATA CAATGATGCC 
CATATCCTGG AGAAGTTACT CAAAGATAAA AGGAAAGAGC TGGGCCCTCT GCCTGATGAT 
GATGACATGG CTTCTCCCAA ACTTAAATTG AGTAGGAAGA GTGGTGTTTC TCCTAAGAAA 
TCAAAGTACA TGACTCCAAT GCAGCAGAAA CTGAATGAAG TGTATGAAGC TGTAAAGAAC 
TATACTGATA AGAGGGGTCG CCGCCTTAGT GCTATATTTC TAAGACTCCC CTCTAGATCA 
GAGCTGCCTG ACTACTACCT GACCATTAAA AAGCCCATGG ACATGGAAAA AATTCGAAGT 
CACATGATGG CAAACAAGTA CCAAGACATA GATTCTATGG TAGAGGACTT TGTCATGATG 
TTTAATAATG CCTGTACCTA CAATGAACCA GAGTCTTTGA TCTACAAAGA TGCCCTTGTA 
CTGCATAAAG TCCTCCTTGA GACTCGGAGA GACCTGGAGG GAGATGAGGA TTCTCATGTC 
CCTAATGTGA CGTTGCTGAT TCAAGAGCTC ATCCATAACC TTTTTGTGTC AGTCATGAGT 
CATCAGGATG ACGAAGGGAG GTGTTACAGC GACTCCTTAG CAGAAATTCC TGCTGTGGAT 
CCCAACTCTC CCAATAAACC TCCCCTTACA TTTGACATTA TCAGGAAAAA TGTTGAAAGT 
AATCGGTATC GGCGACTTGA TTTATTTCAG GAGCATATGT TTGAAGTATT GGAACGGGCA 
AGAAGGATGA ACCGGACAGA TTCCGAAATA TATGAGGATG CTGTAGAACT TCAGCAGTTT 
TTTATTAGAA TTCGTGATGA ACTCTGCAAA AATGGAGAGA TCCTTCTTTC TCCAGCACTC 
AGCTATACCA CAAAACACTT GCATAACGAT GTGGAAAAAG AAAAAAAGGA AAAATTGCCT 
AAAGAAATAG AGGAAGATAA ACTAAAACGC GAAGAAGAAA AAAGAGAAGC TGAAAAAAGT 
GAAGATTCCT CAGGTACTAC AGGCCTCTCA GGCTTACATC GTACATACAG CCAGGACTGC 
AGCTTTAAGA ACAGCATGTA TCATGTCGGA GATTATGTCT ATGTTGAACC TGCGGAGGCC 
AATCTACAAC CACATATAGT GTGTATTGAG AGACTGTGGG AGGATTCAGC TGGTGAAAAA 
TGGTTGTACG GCTGTTGGTT TTATCGGCCA AATGAAACAT TCCATTTGGC TACACGAAAA 
TTTCTAGAAA AAGAAGTTTT TAAGAGTGAC TACTACAATA AAGTACCTGT TAGTAAAATT 
CTAGGCAAAT GTGTAGTCAT GTTTGTCAAG GAATACTTTA AATTATGTCC AGAAAACTTT 
CGCGATGAGG ATGTTTTTGT CTGTGAATCG AGGTATTCTG CCAAAACCAA ATCTTTTAAG 
AAAATTAAAC TGTGGACCAT GCCCATCAGT TCAGTTAGAT TTGTCCCTCG GGATGTGCCT 
TTGCCTGTGG TCCGAGTGGC CTCTGTGTTT GCAAATGCAG ATAAAGGGGA TGATGAGAAG 
AATACAGACA ACTCAGATGA CAATAGAGCT GAAGACAATT TTAACTTGGA AAAGGAAAAA 
GAAGATGTTC CTGTGGAGAT GTCCAATGGT GAGCCAGGTT GCCACTACTT TGAGCAGCTT 
CGGTACAATG ACATGTGGCT GAAGGTTGGT GATTGTGTCT TCATCAAATC CCACGGCTTG 
GTGCGCCCTC GTGTGGGCAG AATTGAGAAA GTATGGGTCC GAGATGGAGC TGCATATTTT 
TATGGCCCTA TCTTCATTCA TCCAGAAGAA ACAGAACATG AGCCCACAAA AATGTTCTAC 
AAAAAAGAAG TGTTTCTGAG TAATCTGGAA GAGACCTGCC CTATGAGTTG TATTCTGGGG 
AAATGTGCAG TGCTGTCATT CAAGGACTTC CTCTCCTGCA GGCCAACTGA AATACCAGAA 
AATGACATTC TGCTTTGTGA GAGCCGCTAT AATGAGAGTG ACAAGCAGAT GAAGAAGTTC 
AAGGGTTTGA AGAGGTTTTC ACTCTCTGCT AAAGTTGTAG ATGATGAAAT CTACTACTTC 
AGAAAACCAA TCATTCCTCA GAAGGAACCC TCACCTTTGT TAGAAAAGAA GATACAATTG 
CTAGAAGCTA AATTTGCAGA GTTAGAAGGA GGAGATGATG ATATTGAGGA GATGGGAGAA 
GAGGATAGTG AAGTCATTGA AGCTCCATCT CTACCTCAAC TGCAGACACC CCTGGCCAAT 
GAGTTGGACC TCATGCCCTA TACACCCCCA CAGCTTTCTG TCTTACAGTC TACCCCAAAG 
TCTGCCAAAG GCAGTGCAAA GAAGGAAAGT TCTAAACGAA AAATCAACAT GAGTGGCTAC 
ATTTTGTTCA GCAGTGAAAT GAGAGCTGTG ATTAAAGCCC AGCACCCAGA CTACTCTTTT 
GGGGAGCTCA GCAGACTGGT GGGGACAGAA TGGAGAAACC TTGAAACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CTCACCATCT TCCGCCAGGT 
GTGCCTGGCC TCCCAGGCAT CCCACCACCG GGTGTGATGA ATCAAGGAGT AGCCCCCATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAAC AGGTAGGAGT TTTGGGACCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGTCCTCATC CAGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACGCCCAT GTTTGTGGCT CCCCCACCAA AGACCCAAAG GCTTCTCCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCTG AATCCAACAG CATTAGCAAG 
TGGGACCAAA CTTTGGCAGC TCGAAGACGG GATGTCCATT TGTCCAAAGA ACAGGAGAGC 
CGCCTACCTT CTCACTGGCT CAAAAGTAAA GGGGCACACA CCACCATGGC AGATGCCCTC 
TGGCGCCTAC GGGATTTAAT GCTTCGAGAC ACTCTCAACA TCCGACAGGC ATACAACCTA 
GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519418.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519481.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X35, mRNA.

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CloneID	OMu50264
Clone ID Related Accession (Same CDS sequence)	XM_006519418.3
Accession Version	XM_006519418.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519418.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGTGT GATGAATCAA GGAGTAGCCC CCATGGTAGG GACTCCAGCA 
CCAGGTGGAA GTCCGTATGG ACAACAGGTA GGAGTTTTGG GACCTCCAGG GCAGCAGGCA 
CCACCTCCAT ATCCTGGTCC TCATCCAGCT GGCCCCCCTG TCATACAGCA GCCAACAACG 
CCCATGTTTG TGGCTCCCCC ACCAAAGACC CAAAGGCTTC TCCACTCAGA GGCCTACCTG 
AAATACATTG AAGGACTCAG TGCTGAATCC AACAGCATTA GCAAGTGGGA CCAAACTTTG 
GCAGCTCGAA GACGGGATGT CCATTTGTCC AAAGAACAGG AGAGCCGCCT ACCTTCTCAC 
TGGCTCAAAA GTAAAGGGGC ACACACCACC ATGGCAGATG CCCTCTGGCG CCTACGGGAT 
TTAATGCTTC GAGACACTCT CAACATCCGA CAGGCATACA ACCTAGAAAA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519481.1
CDS	345..5381
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519418.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519418.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG 
GAGAGAGAGC GAGCAGCACA GCAACAGCAG CCGAGTGCTT CTCCCCGAGC AGGCACCCCT 
GTGGGGGCTC TCATGGGGGT GGTGCCACCA CCAACACCAA TGGGGATGCT CAATCAGCAG 
TTGACACCTG TTGCAGGTGT GATGAATCAA GGAGTAGCCC CCATGGTAGG GACTCCAGCA 
CCAGGTGGAA GTCCGTATGG ACAACAGGTA GGAGTTTTGG GACCTCCAGG GCAGCAGGCA 
CCACCTCCAT ATCCTGGTCC TCATCCAGCT GGCCCCCCTG TCATACAGCA GCCAACAACG 
CCCATGTTTG TGGCTCCCCC ACCAAAGACC CAAAGGCTTC TCCACTCAGA GGCCTACCTG 
AAATACATTG AAGGACTCAG TGCTGAATCC AACAGCATTA GCAAGTGGGA CCAAACTTTG 
GCAGCTCGAA GACGGGATGT CCATTTGTCC AAAGAACAGG AGAGCCGCCT ACCTTCTCAC 
TGGCTCAAAA GTAAAGGGGC ACACACCACC ATGGCAGATG CCCTCTGGCG CCTACGGGAT 
TTAATGCTTC GAGACACTCT CAACATCCGA CAGGCATACA ACCTAGAAAA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519419.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519482.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X36, mRNA.

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CloneID	OMu50265
Clone ID Related Accession (Same CDS sequence)	XM_006519419.2
Accession Version	XM_006519419.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519419.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGGAATA CTCATGACAG TGAGATGTTG GGTCTCAGGA GGCTATCCAG TAAAAAGAAC 
ATAAGAAAAC AGCGAATGAA AATTTTATTC AATGTTGTTC TTGAAGCTCG AGAGCCAGGT 
TCAGGCAGAA GACTTTGCGA TCTATTTATG GTTAAGCCAT CCAAGAAGGA CTATCCTGAT 
TATTATAAAA TCATCTTAGA GCCAATGGAC CTGAAAATAA TTGAGCATAA CATCCGAAAT 
GACAAATATG CAGGTGAAGA AGGAATGATG GAAGACATGA AACTCATGTT CCGCAATGCC 
AGGCACTACA ATGAGGAGGG CTCCCAGGTA TACAATGATG CCCATATCCT GGAGAAGTTA 
CTCAAAGATA AAAGGAAAGA GCTGGGCCCT CTGCCTGATG ATGATGACAT GGCTTCTCCC 
AAACTTAAAT TGAGTAGGAA GAGTGGTGTT TCTCCTAAGA AATCAAAGTA CATGACTCCA 
ATGCAGCAGA AACTGAATGA AGTGTATGAA GCTGTAAAGA ACTATACTGA TAAGAGGGGT 
CGCCGCCTTA GTGCTATATT TCTAAGACTC CCCTCTAGAT CAGAGCTGCC TGACTACTAC 
CTGACCATTA AAAAGCCCAT GGACATGGAA AAAATTCGAA GTCACATGAT GGCAAACAAG 
TACCAAGACA TAGATTCTAT GGTAGAGGAC TTTGTCATGA TGTTTAATAA TGCCTGTACC 
TACAATGAAC CAGAGTCTTT GATCTACAAA GATGCCCTTG TACTGCATAA AGTCCTCCTT 
GAGACTCGGA GAGACCTGGA GGGAGATGAG GATTCTCATG TCCCTAATGT GACGTTGCTG 
ATTCAAGAGC TCATCCATAA CCTTTTTGTG TCAGTCATGA GTCATCAGGA TGACGAAGGG 
AGGTGTTACA GCGACTCCTT AGCAGAAATT CCTGCTGTGG ATCCCAACTC TCCCAATAAA 
CCTCCCCTTA CATTTGACAT TATCAGGAAA AATGTTGAAA GTAATCGGTA TCGGCGACTT 
GATTTATTTC AGGAGCATAT GTTTGAAGTA TTGGAACGGG CAAGAAGGAT GAACCGGACA 
GATTCCGAAA TATATGAGGA TGCTGTAGAA CTTCAGCAGT TTTTTATTAG AATTCGTGAT 
GAACTCTGCA AAAATGGAGA GATCCTTCTT TCTCCAGCAC TCAGCTATAC CACAAAACAC 
TTGCATAACG ATGTGGAAAA AGAAAAAAAG GAAAAATTGC CTAAAGAAAT AGAGGAAGAT 
AAACTAAAAC GCGAAGAAGA AAAAAGAGAA GCTGAAAAAA GTGAAGATTC CTCAGGTACT 
ACAGGCCTCT CAGGCTTACA TCGTACATAC AGCCAGGACT GCAGCTTTAA GAACAGCATG 
TATCATGTCG GAGATTATGT CTATGTTGAA CCTGCGGAGG CCAATCTACA ACCACATATA 
GTGTGTATTG AGAGACTGTG GGAGGATTCA GCTGGTGAAA AATGGTTGTA CGGCTGTTGG 
TTTTATCGGC CAAATGAAAC ATTCCATTTG GCTACACGAA AATTTCTAGA AAAAGAAGTT 
TTTAAGAGTG ACTACTACAA TAAAGTACCT GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC 
ATGTTTGTCA AGGAATACTT TAAATTATGT CCAGAAAACT TTCGCGATGA GGATGTTTTT 
GTCTGTGAAT CGAGGTATTC TGCCAAAACC AAATCTTTTA AGAAAATTAA ACTGTGGACC 
ATGCCCATCA GTTCAGTTAG ATTTGTCCCT CGGGATGTGC CTTTGCCTGT GGTCCGAGTG 
GCCTCTGTGT TTGCAAATGC AGATAAAGGG GATGATGAGA AGAATACAGA CAACTCAGAT 
GACAATAGAG CTGAAGACAA TTTTAACTTG GAAAAGGAAA AAGAAGATGT TCCTGTGGAG 
ATGTCCAATG GTGAGCCAGG TTGCCACTAC TTTGAGCAGC TTCGGTACAA TGACATGTGG 
CTGAAGGTTG GTGATTGTGT CTTCATCAAA TCCCACGGCT TGGTGCGCCC TCGTGTGGGC 
AGAATTGAGA AAGTATGGGT CCGAGATGGA GCTGCATATT TTTATGGCCC TATCTTCATT 
CATCCAGAAG AAACAGAACA TGAGCCCACA AAAATGTTCT ACAAAAAAGA AGTGTTTCTG 
AGTAATCTGG AAGAGACCTG CCCTATGAGT TGTATTCTGG GGAAATGTGC AGTGCTGTCA 
TTCAAGGACT TCCTCTCCTG CAGGCCAACT GAAATACCAG AAAATGACAT TCTGCTTTGT 
GAGAGCCGCT ATAATGAGAG TGACAAGCAG ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT 
TCACTCTCTG CTAAAGTTGT AGATGATGAA ATCTACTACT TCAGAAAACC AATCATTCCT 
CAGAAGGAAC CCTCACCTTT GTTAGAAAAG AAGATACAAT TGCTAGAAGC TAAATTTGCA 
GAGTTAGAAG GAGGAGATGA TGATATTGAG GAGATGGGAG AAGAGGATAG TGAAGTCATT 
GAAGCTCCAT CTCTACCTCA ACTGCAGACA CCCCTGGCCA ATGAGTTGGA CCTCATGCCC 
TATACACCCC CACAGCTTTC TGTCTTACAG TCTACCCCAA AGTCTGCCAA AGGCAGTGCA 
AAGAAGGAAA GTTCTAAACG AAAAATCAAC ATGAGTGGCT ACATTTTGTT CAGCAGTGAA 
ATGAGAGCTG TGATTAAAGC CCAGCACCCA GACTACTCTT TTGGGGAGCT CAGCAGACTG 
GTGGGGACAG AATGGAGAAA CCTTGAAACA GCCAAGAAAG CAGAATATGA AGAGCGGGCA 
GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG CGAGCAGCAC AGCAACAGCA GCCGAGTGCT 
TCTCCCCGAG CAGGCACCCC TGTGGGGGCT CTCATGGGGG TGGTGCCACC ACCAACACCA 
ATGGGGATGC TCAATCAGCA GTTGACACCT GTTGCAGGCA TGATGGGTGG CTATCCGCCA 
GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT GGCCTTGTTA GCATGGGCAG CATGCAGCCA 
CTTCACCCTG GGGGGCCTCC ACCTCACCAT CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC 
ATCCCACCAC CGGGTGTGAT GAATCAAGGA GTAGCCCCCA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CGTATGGACA ACAGGTAGGA GTTTTGGGAC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CTGGTCCTCA TCCAGCTGGC CCCCCTGTCA TACAGCAGCC AACAACGCCC 
ATGTTTGTGG CTCCCCCACC AAAGACCCAA AGGCTTCTCC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC TGAATCCAAC AGCATTAGCA AGTGGGACCA AACTTTGGCA 
GCTCGAAGAC GGGATGTCCA TTTGTCCAAA GAACAGGAGA GCCGCCTACC TTCTCACTGG 
CTCAAAAGTA AAGGGGCACA CACCACCATG GCAGATGCCC TCTGGCGCCT ACGGGATTTA 
ATGCTTCGAG ACACTCTCAA CATCCGACAG GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519482.1
CDS	308..5341
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519419.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519419.2

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCCACCTCT CCTTCCAGCA GTGTCAGTGG AGACTTTGAT 
GACGGGCACC ATTCTGTGCC TACACCAGGC CCAAGCAGGA AAAGGAGAAG ACTGTCCAAT 
CTTCCAACTG TAGATCCTAT TGCTGTGTGC CATGAACTCT ATAACACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT CCTCTGTGAG CTGTTCATTA GGGCTCCAAA GCGGAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTTAAAATGG AAGAGTATGA TGATGTTAAT CTACTGACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTACTA TAAGCCAGAT TCCCCTGAGT ATAAAGCTGC TTGTAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGACGATGAA 
GATGATGACG AAGATGGGCA AGACAATCAA GGCACACTGG CTGACGGCTC TTCTCCAGGT 
TATCTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGGCTCA TCAGTGAACT TTTTCAGAAA CTGCCTTCCA AAGTGCAATA TCCAGACTAT 
TATGCAATAA TTAAGGAACC TATAGATCTC AAGACCATTG CTCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATACACGC AATGGCCAAA GATATAGATC TTCTAGCAAA AAATGCCAAA 
ACATACAATG AGCCTGGGTC TCAAGTATTC AAGGATGCCA ATTCGATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATCAT GAAATGACTA AATCAAGTCT TCGAATAAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGCTTG TCAGCAATTA CCATGGCACT TCAGTATGGA 
TCAGAGAGTG AAGAGGACGC TGCTTTAGCT GCTGCACGCT ATGAAGAAGG GGAATCTGAA 
GCAGAGAGCA TCACTTCCTT CATGGACGTT TCCAACCCCT TTCATCAGCT TTACGACACA 
GTTAGGAGCT GTAGGAATCA CCAAGGGCAG CTCATAGCTG AACCTTTCTT CCATTTGCCT 
TCAAAGAAAA AATACCCAGA TTATTATCAG CAAATTAAAA TGCCCATATC ACTTCAACAG 
ATCAGAACAA AGCTAAAGAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGTTAT AACGTTCCCA ATTCAGCCAT CTATAAGCGA 
GTTCTAAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAGG AGCTTGCGAG GAGAGATGAC 
ATTGAGGACG GAGACAGCAT GATCTCCTCA GCCACTTCTG ACACTGGTAG TGCCAAAAGG 
AAAAGGAATA CTCATGACAG TGAGATGTTG GGTCTCAGGA GGCTATCCAG TAAAAAGAAC 
ATAAGAAAAC AGCGAATGAA AATTTTATTC AATGTTGTTC TTGAAGCTCG AGAGCCAGGT 
TCAGGCAGAA GACTTTGCGA TCTATTTATG GTTAAGCCAT CCAAGAAGGA CTATCCTGAT 
TATTATAAAA TCATCTTAGA GCCAATGGAC CTGAAAATAA TTGAGCATAA CATCCGAAAT 
GACAAATATG CAGGTGAAGA AGGAATGATG GAAGACATGA AACTCATGTT CCGCAATGCC 
AGGCACTACA ATGAGGAGGG CTCCCAGGTA TACAATGATG CCCATATCCT GGAGAAGTTA 
CTCAAAGATA AAAGGAAAGA GCTGGGCCCT CTGCCTGATG ATGATGACAT GGCTTCTCCC 
AAACTTAAAT TGAGTAGGAA GAGTGGTGTT TCTCCTAAGA AATCAAAGTA CATGACTCCA 
ATGCAGCAGA AACTGAATGA AGTGTATGAA GCTGTAAAGA ACTATACTGA TAAGAGGGGT 
CGCCGCCTTA GTGCTATATT TCTAAGACTC CCCTCTAGAT CAGAGCTGCC TGACTACTAC 
CTGACCATTA AAAAGCCCAT GGACATGGAA AAAATTCGAA GTCACATGAT GGCAAACAAG 
TACCAAGACA TAGATTCTAT GGTAGAGGAC TTTGTCATGA TGTTTAATAA TGCCTGTACC 
TACAATGAAC CAGAGTCTTT GATCTACAAA GATGCCCTTG TACTGCATAA AGTCCTCCTT 
GAGACTCGGA GAGACCTGGA GGGAGATGAG GATTCTCATG TCCCTAATGT GACGTTGCTG 
ATTCAAGAGC TCATCCATAA CCTTTTTGTG TCAGTCATGA GTCATCAGGA TGACGAAGGG 
AGGTGTTACA GCGACTCCTT AGCAGAAATT CCTGCTGTGG ATCCCAACTC TCCCAATAAA 
CCTCCCCTTA CATTTGACAT TATCAGGAAA AATGTTGAAA GTAATCGGTA TCGGCGACTT 
GATTTATTTC AGGAGCATAT GTTTGAAGTA TTGGAACGGG CAAGAAGGAT GAACCGGACA 
GATTCCGAAA TATATGAGGA TGCTGTAGAA CTTCAGCAGT TTTTTATTAG AATTCGTGAT 
GAACTCTGCA AAAATGGAGA GATCCTTCTT TCTCCAGCAC TCAGCTATAC CACAAAACAC 
TTGCATAACG ATGTGGAAAA AGAAAAAAAG GAAAAATTGC CTAAAGAAAT AGAGGAAGAT 
AAACTAAAAC GCGAAGAAGA AAAAAGAGAA GCTGAAAAAA GTGAAGATTC CTCAGGTACT 
ACAGGCCTCT CAGGCTTACA TCGTACATAC AGCCAGGACT GCAGCTTTAA GAACAGCATG 
TATCATGTCG GAGATTATGT CTATGTTGAA CCTGCGGAGG CCAATCTACA ACCACATATA 
GTGTGTATTG AGAGACTGTG GGAGGATTCA GCTGGTGAAA AATGGTTGTA CGGCTGTTGG 
TTTTATCGGC CAAATGAAAC ATTCCATTTG GCTACACGAA AATTTCTAGA AAAAGAAGTT 
TTTAAGAGTG ACTACTACAA TAAAGTACCT GTTAGTAAAA TTCTAGGCAA ATGTGTAGTC 
ATGTTTGTCA AGGAATACTT TAAATTATGT CCAGAAAACT TTCGCGATGA GGATGTTTTT 
GTCTGTGAAT CGAGGTATTC TGCCAAAACC AAATCTTTTA AGAAAATTAA ACTGTGGACC 
ATGCCCATCA GTTCAGTTAG ATTTGTCCCT CGGGATGTGC CTTTGCCTGT GGTCCGAGTG 
GCCTCTGTGT TTGCAAATGC AGATAAAGGG GATGATGAGA AGAATACAGA CAACTCAGAT 
GACAATAGAG CTGAAGACAA TTTTAACTTG GAAAAGGAAA AAGAAGATGT TCCTGTGGAG 
ATGTCCAATG GTGAGCCAGG TTGCCACTAC TTTGAGCAGC TTCGGTACAA TGACATGTGG 
CTGAAGGTTG GTGATTGTGT CTTCATCAAA TCCCACGGCT TGGTGCGCCC TCGTGTGGGC 
AGAATTGAGA AAGTATGGGT CCGAGATGGA GCTGCATATT TTTATGGCCC TATCTTCATT 
CATCCAGAAG AAACAGAACA TGAGCCCACA AAAATGTTCT ACAAAAAAGA AGTGTTTCTG 
AGTAATCTGG AAGAGACCTG CCCTATGAGT TGTATTCTGG GGAAATGTGC AGTGCTGTCA 
TTCAAGGACT TCCTCTCCTG CAGGCCAACT GAAATACCAG AAAATGACAT TCTGCTTTGT 
GAGAGCCGCT ATAATGAGAG TGACAAGCAG ATGAAGAAGT TCAAGGGTTT GAAGAGGTTT 
TCACTCTCTG CTAAAGTTGT AGATGATGAA ATCTACTACT TCAGAAAACC AATCATTCCT 
CAGAAGGAAC CCTCACCTTT GTTAGAAAAG AAGATACAAT TGCTAGAAGC TAAATTTGCA 
GAGTTAGAAG GAGGAGATGA TGATATTGAG GAGATGGGAG AAGAGGATAG TGAAGTCATT 
GAAGCTCCAT CTCTACCTCA ACTGCAGACA CCCCTGGCCA ATGAGTTGGA CCTCATGCCC 
TATACACCCC CACAGCTTTC TGTCTTACAG TCTACCCCAA AGTCTGCCAA AGGCAGTGCA 
AAGAAGGAAA GTTCTAAACG AAAAATCAAC ATGAGTGGCT ACATTTTGTT CAGCAGTGAA 
ATGAGAGCTG TGATTAAAGC CCAGCACCCA GACTACTCTT TTGGGGAGCT CAGCAGACTG 
GTGGGGACAG AATGGAGAAA CCTTGAAACA GCCAAGAAAG CAGAATATGA AGAGCGGGCA 
GCTAAAGTTG CTGAGCAGCA GGAGAGAGAG CGAGCAGCAC AGCAACAGCA GCCGAGTGCT 
TCTCCCCGAG CAGGCACCCC TGTGGGGGCT CTCATGGGGG TGGTGCCACC ACCAACACCA 
ATGGGGATGC TCAATCAGCA GTTGACACCT GTTGCAGGCA TGATGGGTGG CTATCCGCCA 
GGCCTTCCAC CTTTGCAGGG CCCAGTTGAT GGCCTTGTTA GCATGGGCAG CATGCAGCCA 
CTTCACCCTG GGGGGCCTCC ACCTCACCAT CTTCCGCCAG GTGTGCCTGG CCTCCCAGGC 
ATCCCACCAC CGGGTGTGAT GAATCAAGGA GTAGCCCCCA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CGTATGGACA ACAGGTAGGA GTTTTGGGAC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CTGGTCCTCA TCCAGCTGGC CCCCCTGTCA TACAGCAGCC AACAACGCCC 
ATGTTTGTGG CTCCCCCACC AAAGACCCAA AGGCTTCTCC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC TGAATCCAAC AGCATTAGCA AGTGGGACCA AACTTTGGCA 
GCTCGAAGAC GGGATGTCCA TTTGTCCAAA GAACAGGAGA GCCGCCTACC TTCTCACTGG 
CTCAAAAGTA AAGGGGCACA CACCACCATG GCAGATGCCC TCTGGCGCCT ACGGGATTTA 
ATGCTTCGAG ACACTCTCAA CATCCGACAG GCATACAACC TAGAAAATGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519420.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519483.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X37, mRNA.

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CloneID	OMu50266
Clone ID Related Accession (Same CDS sequence)	XM_006519420.3
Accession Version	XM_006519420.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519420.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519483.1
CDS	345..5372
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519420.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519420.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519421.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519484.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X38, mRNA.

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CloneID	OMu50267
Clone ID Related Accession (Same CDS sequence)	XM_006519421.3
Accession Version	XM_006519421.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519421.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGTCTACC 
CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG GAAAGTTCTA AACGAAAAAT CAACATGAGT 
GGCTACATTT TGTTCAGCAG TGAAATGAGA GCTGTGATTA AAGCCCAGCA CCCAGACTAC 
TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG ACAGAATGGA GAAACCTTGA AACAGCCAAG 
AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA 
GCACAGCAAC AGCAGCCGAG TGCTTCTCCC CGAGCAGGCA CCCCTGTGGG GGCTCTCATG 
GGGGTGGTGC CACCACCAAC ACCAATGGGG ATGCTCAATC AGCAGTTGAC ACCTGTTGCA 
GGTGTGATGA ATCAAGGAGT AGCCCCCATG GTAGGGACTC CAGCACCAGG TGGAAGTCCG 
TATGGACAAC AGGTAGGAGT TTTGGGACCT CCAGGGCAGC AGGCACCACC TCCATATCCT 
GGTCCTCATC CAGCTGGCCC CCCTGTCATA CAGCAGCCAA CAACGCCCAT GTTTGTGGCT 
CCCCCACCAA AGACCCAAAG GCTTCTCCAC TCAGAGGCCT ACCTGAAATA CATTGAAGGA 
CTCAGTGCTG AATCCAACAG CATTAGCAAG TGGGACCAAA CTTTGGCAGC TCGAAGACGG 
GATGTCCATT TGTCCAAAGA ACAGGAGAGC CGCCTACCTT CTCACTGGCT CAAAAGTAAA 
GGGGCACACA CCACCATGGC AGATGCCCTC TGGCGCCTAC GGGATTTAAT GCTTCGAGAC 
ACTCTCAACA TCCGACAGGC ATACAACCTA GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519484.1
CDS	345..5366
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519421.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519421.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGTCTACC 
CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG GAAAGTTCTA AACGAAAAAT CAACATGAGT 
GGCTACATTT TGTTCAGCAG TGAAATGAGA GCTGTGATTA AAGCCCAGCA CCCAGACTAC 
TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG ACAGAATGGA GAAACCTTGA AACAGCCAAG 
AAAGCAGAAT ATGAAGAGCG GGCAGCTAAA GTTGCTGAGC AGCAGGAGAG AGAGCGAGCA 
GCACAGCAAC AGCAGCCGAG TGCTTCTCCC CGAGCAGGCA CCCCTGTGGG GGCTCTCATG 
GGGGTGGTGC CACCACCAAC ACCAATGGGG ATGCTCAATC AGCAGTTGAC ACCTGTTGCA 
GGTGTGATGA ATCAAGGAGT AGCCCCCATG GTAGGGACTC CAGCACCAGG TGGAAGTCCG 
TATGGACAAC AGGTAGGAGT TTTGGGACCT CCAGGGCAGC AGGCACCACC TCCATATCCT 
GGTCCTCATC CAGCTGGCCC CCCTGTCATA CAGCAGCCAA CAACGCCCAT GTTTGTGGCT 
CCCCCACCAA AGACCCAAAG GCTTCTCCAC TCAGAGGCCT ACCTGAAATA CATTGAAGGA 
CTCAGTGCTG AATCCAACAG CATTAGCAAG TGGGACCAAA CTTTGGCAGC TCGAAGACGG 
GATGTCCATT TGTCCAAAGA ACAGGAGAGC CGCCTACCTT CTCACTGGCT CAAAAGTAAA 
GGGGCACACA CCACCATGGC AGATGCCCTC TGGCGCCTAC GGGATTTAAT GCTTCGAGAC 
ACTCTCAACA TCCGACAGGC ATACAACCTA GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519422.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519485.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X39, mRNA.

Price & Availability↑

CloneID	OMu50268
Clone ID Related Accession (Same CDS sequence)	XM_006519422.3
Accession Version	XM_006519422.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519422.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGTCTACC 
CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG GAAAGTTCTA AACGAAAAAT CAACATGAGT 
GGCTACATTT TGTTCAGCAG TGAAATGAGA GCTGTGATTA AAGCCCAGCA CCCAGACTAC 
TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG ACAGAATGGA GAAACCTTGA AACAGCCAAG 
AAAGCAGAAT ATGAAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519485.1
CDS	345..5357
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519422.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519422.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGTCTACC 
CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG GAAAGTTCTA AACGAAAAAT CAACATGAGT 
GGCTACATTT TGTTCAGCAG TGAAATGAGA GCTGTGATTA AAGCCCAGCA CCCAGACTAC 
TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG ACAGAATGGA GAAACCTTGA AACAGCCAAG 
AAAGCAGAAT ATGAAGGCAT GATGGGTGGC TATCCGCCAG GCCTTCCACC TTTGCAGGGC 
CCAGTTGATG GCCTTGTTAG CATGGGCAGC ATGCAGCCAC TTCACCCTGG GGGGCCTCCA 
CCTCACCATC TTCCGCCAGG TGTGCCTGGC CTCCCAGGCA TCCCACCACC GGGTGTGATG 
AATCAAGGAG TAGCCCCCAT GGTAGGGACT CCAGCACCAG GTGGAAGTCC GTATGGACAA 
CAGGTAGGAG TTTTGGGACC TCCAGGGCAG CAGGCACCAC CTCCATATCC TGGTCCTCAT 
CCAGCTGGCC CCCCTGTCAT ACAGCAGCCA ACAACGCCCA TGTTTGTGGC TCCCCCACCA 
AAGACCCAAA GGCTTCTCCA CTCAGAGGCC TACCTGAAAT ACATTGAAGG ACTCAGTGCT 
GAATCCAACA GCATTAGCAA GTGGGACCAA ACTTTGGCAG CTCGAAGACG GGATGTCCAT 
TTGTCCAAAG AACAGGAGAG CCGCCTACCT TCTCACTGGC TCAAAAGTAA AGGGGCACAC 
ACCACCATGG CAGATGCCCT CTGGCGCCTA CGGGATTTAA TGCTTCGAGA CACTCTCAAC 
ATCCGACAGG CATACAACCT AGAAAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519423.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519486.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X43, mRNA.

Price & Availability↑

CloneID	OMu50269
Clone ID Related Accession (Same CDS sequence)	XM_006519423.3
Accession Version	XM_006519423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519423.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519486.1
CDS	358..5325
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519423.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X43, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519423.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GGCATGATGG GTGGCTATCC GCCAGGCCTT 
CCACCTTTGC AGGGCCCAGT TGATGGCCTT GTTAGCATGG GCAGCATGCA GCCACTTCAC 
CCTGGGGGGC CTCCACCTCA CCATCTTCCG CCAGGTGTGC CTGGCCTCCC AGGCATCCCA 
CCACCGGGTG TGATGAATCA AGGAGTAGCC CCCATGGTAG GGACTCCAGC ACCAGGTGGA 
AGTCCGTATG GACAACAGGT AGGAGTTTTG GGACCTCCAG GGCAGCAGGC ACCACCTCCA 
TATCCTGGTC CTCATCCAGC TGGCCCCCCT GTCATACAGC AGCCAACAAC GCCCATGTTT 
GTGGCTCCCC CACCAAAGAC CCAAAGGCTT CTCCACTCAG AGGCCTACCT GAAATACATT 
GAAGGACTCA GTGCTGAATC CAACAGCATT AGCAAGTGGG ACCAAACTTT GGCAGCTCGA 
AGACGGGATG TCCATTTGTC CAAAGAACAG GAGAGCCGCC TACCTTCTCA CTGGCTCAAA 
AGTAAAGGGG CACACACCAC CATGGCAGAT GCCCTCTGGC GCCTACGGGA TTTAATGCTT 
CGAGACACTC TCAACATCCG ACAGGCATAC AACCTAGAAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519424.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519487.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X45, mRNA.

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CloneID	OMu50270
Clone ID Related Accession (Same CDS sequence)	XM_006519424.3
Accession Version	XM_006519424.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519424.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGTA AAAAGAACAT AAGAAAACAG CGAATGAAAA TTTTATTCAA TGTTGTTCTT 
GAAGCTCGAG AGCCAGGTTC AGGCAGAAGA CTTTGCGATC TATTTATGGT TAAGCCATCC 
AAGAAGGACT ATCCTGATTA TTATAAAATC ATCTTAGAGC CAATGGACCT GAAAATAATT 
GAGCATAACA TCCGAAATGA CAAATATGCA GGTGAAGAAG GAATGATGGA AGACATGAAA 
CTCATGTTCC GCAATGCCAG GCACTACAAT GAGGAGGGCT CCCAGGTATA CAATGATGCC 
CATATCCTGG AGAAGTTACT CAAAGATAAA AGGAAAGAGC TGGGCCCTCT GCCTGATGAT 
GATGACATGG CTTCTCCCAA ACTTAAATTG AGTAGGAAGA GTGGTGTTTC TCCTAAGAAA 
TCAAAGTACA TGACTCCAAT GCAGCAGAAA CTGAATGAAG TGTATGAAGC TGTAAAGAAC 
TATACTGATA AGAGGGGTCG CCGCCTTAGT GCTATATTTC TAAGACTCCC CTCTAGATCA 
GAGCTGCCTG ACTACTACCT GACCATTAAA AAGCCCATGG ACATGGAAAA AATTCGAAGT 
CACATGATGG CAAACAAGTA CCAAGACATA GATTCTATGG TAGAGGACTT TGTCATGATG 
TTTAATAATG CCTGTACCTA CAATGAACCA GAGTCTTTGA TCTACAAAGA TGCCCTTGTA 
CTGCATAAAG TCCTCCTTGA GACTCGGAGA GACCTGGAGG GAGATGAGGA TTCTCATGTC 
CCTAATGTGA CGTTGCTGAT TCAAGAGCTC ATCCATAACC TTTTTGTGTC AGTCATGAGT 
CATCAGGATG ACGAAGGGAG GTGTTACAGC GACTCCTTAG CAGAAATTCC TGCTGTGGAT 
CCCAACTCTC CCAATAAACC TCCCCTTACA TTTGACATTA TCAGGAAAAA TGTTGAAAGT 
AATCGGTATC GGCGACTTGA TTTATTTCAG GAGCATATGT TTGAAGTATT GGAACGGGCA 
AGAAGGATGA ACCGGACAGA TTCCGAAATA TATGAGGATG CTGTAGAACT TCAGCAGTTT 
TTTATTAGAA TTCGTGATGA ACTCTGCAAA AATGGAGAGA TCCTTCTTTC TCCAGCACTC 
AGCTATACCA CAAAACACTT GCATAACGAT GTGGAAAAAG AAAAAAAGGA AAAATTGCCT 
AAAGAAATAG AGGAAGATAA ACTAAAACGC GAAGAAGAAA AAAGAGAAGC TGAAAAAAGT 
GAAGATTCCT CAGGTACTAC AGGCCTCTCA GGCTTACATC GTACATACAG CCAGGACTGC 
AGCTTTAAGA ACAGCATGTA TCATGTCGGA GATTATGTCT ATGTTGAACC TGCGGAGGCC 
AATCTACAAC CACATATAGT GTGTATTGAG AGACTGTGGG AGGATTCAGC TGGTGAAAAA 
TGGTTGTACG GCTGTTGGTT TTATCGGCCA AATGAAACAT TCCATTTGGC TACACGAAAA 
TTTCTAGAAA AAGAAGTTTT TAAGAGTGAC TACTACAATA AAGTACCTGT TAGTAAAATT 
CTAGGCAAAT GTGTAGTCAT GTTTGTCAAG GAATACTTTA AATTATGTCC AGAAAACTTT 
CGCGATGAGG ATGTTTTTGT CTGTGAATCG AGGTATTCTG CCAAAACCAA ATCTTTTAAG 
AAAATTAAAC TGTGGACCAT GCCCATCAGT TCAGTTAGAT TTGTCCCTCG GGATGTGCCT 
TTGCCTGTGG TCCGAGTGGC CTCTGTGTTT GCAAATGCAG ATAAAGGGGA TGATGAGAAG 
AATACAGACA ACTCAGATGA CAATAGAGCT GAAGACAATT TTAACTTGGA AAAGGAAAAA 
GAAGATGTTC CTGTGGAGAT GTCCAATGGT GAGCCAGGTT GCCACTACTT TGAGCAGCTT 
CGGTACAATG ACATGTGGCT GAAGGTTGGT GATTGTGTCT TCATCAAATC CCACGGCTTG 
GTGCGCCCTC GTGTGGGCAG AATTGAGAAA GTATGGGTCC GAGATGGAGC TGCATATTTT 
TATGGCCCTA TCTTCATTCA TCCAGAAGAA ACAGAACATG AGCCCACAAA AATGTTCTAC 
AAAAAAGAAG TGTTTCTGAG TAATCTGGAA GAGACCTGCC CTATGAGTTG TATTCTGGGG 
AAATGTGCAG TGCTGTCATT CAAGGACTTC CTCTCCTGCA GGCCAACTGA AATACCAGAA 
AATGACATTC TGCTTTGTGA GAGCCGCTAT AATGAGAGTG ACAAGCAGAT GAAGAAGTTC 
AAGGGTTTGA AGAGGTTTTC ACTCTCTGCT AAAGTTGTAG ATGATGAAAT CTACTACTTC 
AGAAAACCAA TCATTCCTCA GAAGGAACCC TCACCTTTGT TAGAAAAGAA GATACAATTG 
CTAGAAGCTA AATTTGCAGA GTTAGAAGGA GGAGATGATG ATATTGAGGA GATGGGAGAA 
GAGGATAGTG AAGTCATTGA AGCTCCATCT CTACCTCAAC TGCAGACACC CCTGGCCAAT 
GAGTTGGACC TCATGCCCTA TACACCCCCA CAGTCTACCC CAAAGTCTGC CAAAGGCAGT 
GCAAAGAAGG AAAGTTCTAA ACGAAAAATC AACATGAGTG GCTACATTTT GTTCAGCAGT 
GAAATGAGAG CTGTGATTAA AGCCCAGCAC CCAGACTACT CTTTTGGGGA GCTCAGCAGA 
CTGGTGGGGA CAGAATGGAG AAACCTTGAA ACAGCCAAGA AAGCAGAATA TGAAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CTCACCATCT TCCGCCAGGT 
GTGCCTGGCC TCCCAGGCAT CCCACCACCG GGTGTGATGA ATCAAGGAGT AGCCCCCATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAAC AGGTAGGAGT TTTGGGACCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGTCCTCATC CAGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACGCCCAT GTTTGTGGCT CCCCCACCAA AGACCCAAAG GCTTCTCCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCTG AATCCAACAG CATTAGCAAG 
TGGGACCAAA CTTTGGCAGC TCGAAGACGG GATGTCCATT TGTCCAAAGA ACAGGAGAGC 
CGCCTACCTT CTCACTGGCT CAAAAGTAAA GGGGCACACA CCACCATGGC AGATGCCCTC 
TGGCGCCTAC GGGATTTAAT GCTTCGAGAC ACTCTCAACA TCCGACAGGC ATACAACCTA 
GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519487.1
CDS	343..5214
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRISNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519424.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X45, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519424.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGTAATAAAA GAGCAGTCCA AGGGGGTCGC TTGTCAGCAA TTACCATGGC ACTTCAGTAT 
GGATCAGAGA GTGAAGAGGA CGCTGCTTTA GCTGCTGCAC GCTATGAAGA AGGGGAATCT 
GAAGCAGAGA GCATCACTTC CTTCATGGAC GTTTCCAACC CCTTTCATCA GCTTTACGAC 
ACAGTTAGGA GCTGTAGGAA TCACCAAGGG CAGCTCATAG CTGAACCTTT CTTCCATTTG 
CCTTCAAAGA AAAAATACCC AGATTATTAT CAGCAAATTA AAATGCCCAT ATCACTTCAA 
CAGATCAGAA CAAAGCTAAA GAACCAAGAA TATGAAACTT TAGATCATTT GGAGTGTGAT 
CTGAATTTAA TGTTTGAAAA TGCCAAACGT TATAACGTTC CCAATTCAGC CATCTATAAG 
CGAGTTCTAA AACTGCAGCA AGTCATGCAG GCAAAGAAGA AGGAGCTTGC GAGGAGAGAT 
GACATTGAGG ACGGAGACAG CATGATCTCC TCAGCCACTT CTGACACTGG TAGTGCCAAA 
AGGAAAAGTA AAAAGAACAT AAGAAAACAG CGAATGAAAA TTTTATTCAA TGTTGTTCTT 
GAAGCTCGAG AGCCAGGTTC AGGCAGAAGA CTTTGCGATC TATTTATGGT TAAGCCATCC 
AAGAAGGACT ATCCTGATTA TTATAAAATC ATCTTAGAGC CAATGGACCT GAAAATAATT 
GAGCATAACA TCCGAAATGA CAAATATGCA GGTGAAGAAG GAATGATGGA AGACATGAAA 
CTCATGTTCC GCAATGCCAG GCACTACAAT GAGGAGGGCT CCCAGGTATA CAATGATGCC 
CATATCCTGG AGAAGTTACT CAAAGATAAA AGGAAAGAGC TGGGCCCTCT GCCTGATGAT 
GATGACATGG CTTCTCCCAA ACTTAAATTG AGTAGGAAGA GTGGTGTTTC TCCTAAGAAA 
TCAAAGTACA TGACTCCAAT GCAGCAGAAA CTGAATGAAG TGTATGAAGC TGTAAAGAAC 
TATACTGATA AGAGGGGTCG CCGCCTTAGT GCTATATTTC TAAGACTCCC CTCTAGATCA 
GAGCTGCCTG ACTACTACCT GACCATTAAA AAGCCCATGG ACATGGAAAA AATTCGAAGT 
CACATGATGG CAAACAAGTA CCAAGACATA GATTCTATGG TAGAGGACTT TGTCATGATG 
TTTAATAATG CCTGTACCTA CAATGAACCA GAGTCTTTGA TCTACAAAGA TGCCCTTGTA 
CTGCATAAAG TCCTCCTTGA GACTCGGAGA GACCTGGAGG GAGATGAGGA TTCTCATGTC 
CCTAATGTGA CGTTGCTGAT TCAAGAGCTC ATCCATAACC TTTTTGTGTC AGTCATGAGT 
CATCAGGATG ACGAAGGGAG GTGTTACAGC GACTCCTTAG CAGAAATTCC TGCTGTGGAT 
CCCAACTCTC CCAATAAACC TCCCCTTACA TTTGACATTA TCAGGAAAAA TGTTGAAAGT 
AATCGGTATC GGCGACTTGA TTTATTTCAG GAGCATATGT TTGAAGTATT GGAACGGGCA 
AGAAGGATGA ACCGGACAGA TTCCGAAATA TATGAGGATG CTGTAGAACT TCAGCAGTTT 
TTTATTAGAA TTCGTGATGA ACTCTGCAAA AATGGAGAGA TCCTTCTTTC TCCAGCACTC 
AGCTATACCA CAAAACACTT GCATAACGAT GTGGAAAAAG AAAAAAAGGA AAAATTGCCT 
AAAGAAATAG AGGAAGATAA ACTAAAACGC GAAGAAGAAA AAAGAGAAGC TGAAAAAAGT 
GAAGATTCCT CAGGTACTAC AGGCCTCTCA GGCTTACATC GTACATACAG CCAGGACTGC 
AGCTTTAAGA ACAGCATGTA TCATGTCGGA GATTATGTCT ATGTTGAACC TGCGGAGGCC 
AATCTACAAC CACATATAGT GTGTATTGAG AGACTGTGGG AGGATTCAGC TGGTGAAAAA 
TGGTTGTACG GCTGTTGGTT TTATCGGCCA AATGAAACAT TCCATTTGGC TACACGAAAA 
TTTCTAGAAA AAGAAGTTTT TAAGAGTGAC TACTACAATA AAGTACCTGT TAGTAAAATT 
CTAGGCAAAT GTGTAGTCAT GTTTGTCAAG GAATACTTTA AATTATGTCC AGAAAACTTT 
CGCGATGAGG ATGTTTTTGT CTGTGAATCG AGGTATTCTG CCAAAACCAA ATCTTTTAAG 
AAAATTAAAC TGTGGACCAT GCCCATCAGT TCAGTTAGAT TTGTCCCTCG GGATGTGCCT 
TTGCCTGTGG TCCGAGTGGC CTCTGTGTTT GCAAATGCAG ATAAAGGGGA TGATGAGAAG 
AATACAGACA ACTCAGATGA CAATAGAGCT GAAGACAATT TTAACTTGGA AAAGGAAAAA 
GAAGATGTTC CTGTGGAGAT GTCCAATGGT GAGCCAGGTT GCCACTACTT TGAGCAGCTT 
CGGTACAATG ACATGTGGCT GAAGGTTGGT GATTGTGTCT TCATCAAATC CCACGGCTTG 
GTGCGCCCTC GTGTGGGCAG AATTGAGAAA GTATGGGTCC GAGATGGAGC TGCATATTTT 
TATGGCCCTA TCTTCATTCA TCCAGAAGAA ACAGAACATG AGCCCACAAA AATGTTCTAC 
AAAAAAGAAG TGTTTCTGAG TAATCTGGAA GAGACCTGCC CTATGAGTTG TATTCTGGGG 
AAATGTGCAG TGCTGTCATT CAAGGACTTC CTCTCCTGCA GGCCAACTGA AATACCAGAA 
AATGACATTC TGCTTTGTGA GAGCCGCTAT AATGAGAGTG ACAAGCAGAT GAAGAAGTTC 
AAGGGTTTGA AGAGGTTTTC ACTCTCTGCT AAAGTTGTAG ATGATGAAAT CTACTACTTC 
AGAAAACCAA TCATTCCTCA GAAGGAACCC TCACCTTTGT TAGAAAAGAA GATACAATTG 
CTAGAAGCTA AATTTGCAGA GTTAGAAGGA GGAGATGATG ATATTGAGGA GATGGGAGAA 
GAGGATAGTG AAGTCATTGA AGCTCCATCT CTACCTCAAC TGCAGACACC CCTGGCCAAT 
GAGTTGGACC TCATGCCCTA TACACCCCCA CAGTCTACCC CAAAGTCTGC CAAAGGCAGT 
GCAAAGAAGG AAAGTTCTAA ACGAAAAATC AACATGAGTG GCTACATTTT GTTCAGCAGT 
GAAATGAGAG CTGTGATTAA AGCCCAGCAC CCAGACTACT CTTTTGGGGA GCTCAGCAGA 
CTGGTGGGGA CAGAATGGAG AAACCTTGAA ACAGCCAAGA AAGCAGAATA TGAAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CTCACCATCT TCCGCCAGGT 
GTGCCTGGCC TCCCAGGCAT CCCACCACCG GGTGTGATGA ATCAAGGAGT AGCCCCCATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAAC AGGTAGGAGT TTTGGGACCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGTCCTCATC CAGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACGCCCAT GTTTGTGGCT CCCCCACCAA AGACCCAAAG GCTTCTCCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCTG AATCCAACAG CATTAGCAAG 
TGGGACCAAA CTTTGGCAGC TCGAAGACGG GATGTCCATT TGTCCAAAGA ACAGGAGAGC 
CGCCTACCTT CTCACTGGCT CAAAAGTAAA GGGGCACACA CCACCATGGC AGATGCCCTC 
TGGCGCCTAC GGGATTTAAT GCTTCGAGAC ACTCTCAACA TCCGACAGGC ATACAACCTA 
GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519425.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519488.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X46, mRNA.

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CloneID	OMu50271
Clone ID Related Accession (Same CDS sequence)	XM_006519425.3
Accession Version	XM_006519425.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519425.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GGTGTGATGA ATCAAGGAGT AGCCCCCATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAAC AGGTAGGAGT TTTGGGACCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGTCCTCATC CAGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACGCCCAT GTTTGTGGCT CCCCCACCAA AGACCCAAAG GCTTCTCCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCTG AATCCAACAG CATTAGCAAG 
TGGGACCAAA CTTTGGCAGC TCGAAGACGG GATGTCCATT TGTCCAAAGA ACAGGAGAGC 
CGCCTACCTT CTCACTGGCT CAAAAGTAAA GGGGCACACA CCACCATGGC AGATGCCCTC 
TGGCGCCTAC GGGATTTAAT GCTTCGAGAC ACTCTCAACA TCCGACAGGC ATACAACCTA 
GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519488.1
CDS	345..5216
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQLSVLQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519425.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X46, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519425.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGCTTTCT 
GTCTTACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GGTGTGATGA ATCAAGGAGT AGCCCCCATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAAC AGGTAGGAGT TTTGGGACCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGTCCTCATC CAGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACGCCCAT GTTTGTGGCT CCCCCACCAA AGACCCAAAG GCTTCTCCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCTG AATCCAACAG CATTAGCAAG 
TGGGACCAAA CTTTGGCAGC TCGAAGACGG GATGTCCATT TGTCCAAAGA ACAGGAGAGC 
CGCCTACCTT CTCACTGGCT CAAAAGTAAA GGGGCACACA CCACCATGGC AGATGCCCTC 
TGGCGCCTAC GGGATTTAAT GCTTCGAGAC ACTCTCAACA TCCGACAGGC ATACAACCTA 
GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519426.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519489.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X47, mRNA.

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CloneID	OMu50272
Clone ID Related Accession (Same CDS sequence)	XM_006519426.3
Accession Version	XM_006519426.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGTCTACC 
CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG GAAAGTTCTA AACGAAAAAT CAACATGAGT 
GGCTACATTT TGTTCAGCAG TGAAATGAGA GCTGTGATTA AAGCCCAGCA CCCAGACTAC 
TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG ACAGAATGGA GAAACCTTGA AACAGCCAAG 
AAAGCAGAAT ATGAAGGTGT GATGAATCAA GGAGTAGCCC CCATGGTAGG GACTCCAGCA 
CCAGGTGGAA GTCCGTATGG ACAACAGGTA GGAGTTTTGG GACCTCCAGG GCAGCAGGCA 
CCACCTCCAT ATCCTGGTCC TCATCCAGCT GGCCCCCCTG TCATACAGCA GCCAACAACG 
CCCATGTTTG TGGCTCCCCC ACCAAAGACC CAAAGGCTTC TCCACTCAGA GGCCTACCTG 
AAATACATTG AAGGACTCAG TGCTGAATCC AACAGCATTA GCAAGTGGGA CCAAACTTTG 
GCAGCTCGAA GACGGGATGT CCATTTGTCC AAAGAACAGG AGAGCCGCCT ACCTTCTCAC 
TGGCTCAAAA GTAAAGGGGC ACACACCACC ATGGCAGATG CCCTCTGGCG CCTACGGGAT 
TTAATGCTTC GAGACACTCT CAACATCCGA CAGGCATACA ACCTAGAAAA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519489.1
CDS	345..5201
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519426.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X47, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519426.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGGAATAC TCATGACAGT 
GAGATGTTGG GTCTCAGGAG GCTATCCAGT AAAAAGAACA TAAGAAAACA GCGAATGAAA 
ATTTTATTCA ATGTTGTTCT TGAAGCTCGA GAGCCAGGTT CAGGCAGAAG ACTTTGCGAT 
CTATTTATGG TTAAGCCATC CAAGAAGGAC TATCCTGATT ATTATAAAAT CATCTTAGAG 
CCAATGGACC TGAAAATAAT TGAGCATAAC ATCCGAAATG ACAAATATGC AGGTGAAGAA 
GGAATGATGG AAGACATGAA ACTCATGTTC CGCAATGCCA GGCACTACAA TGAGGAGGGC 
TCCCAGGTAT ACAATGATGC CCATATCCTG GAGAAGTTAC TCAAAGATAA AAGGAAAGAG 
CTGGGCCCTC TGCCTGATGA TGATGACATG GCTTCTCCCA AACTTAAATT GAGTAGGAAG 
AGTGGTGTTT CTCCTAAGAA ATCAAAGTAC ATGACTCCAA TGCAGCAGAA ACTGAATGAA 
GTGTATGAAG CTGTAAAGAA CTATACTGAT AAGAGGGGTC GCCGCCTTAG TGCTATATTT 
CTAAGACTCC CCTCTAGATC AGAGCTGCCT GACTACTACC TGACCATTAA AAAGCCCATG 
GACATGGAAA AAATTCGAAG TCACATGATG GCAAACAAGT ACCAAGACAT AGATTCTATG 
GTAGAGGACT TTGTCATGAT GTTTAATAAT GCCTGTACCT ACAATGAACC AGAGTCTTTG 
ATCTACAAAG ATGCCCTTGT ACTGCATAAA GTCCTCCTTG AGACTCGGAG AGACCTGGAG 
GGAGATGAGG ATTCTCATGT CCCTAATGTG ACGTTGCTGA TTCAAGAGCT CATCCATAAC 
CTTTTTGTGT CAGTCATGAG TCATCAGGAT GACGAAGGGA GGTGTTACAG CGACTCCTTA 
GCAGAAATTC CTGCTGTGGA TCCCAACTCT CCCAATAAAC CTCCCCTTAC ATTTGACATT 
ATCAGGAAAA ATGTTGAAAG TAATCGGTAT CGGCGACTTG ATTTATTTCA GGAGCATATG 
TTTGAAGTAT TGGAACGGGC AAGAAGGATG AACCGGACAG ATTCCGAAAT ATATGAGGAT 
GCTGTAGAAC TTCAGCAGTT TTTTATTAGA ATTCGTGATG AACTCTGCAA AAATGGAGAG 
ATCCTTCTTT CTCCAGCACT CAGCTATACC ACAAAACACT TGCATAACGA TGTGGAAAAA 
GAAAAAAAGG AAAAATTGCC TAAAGAAATA GAGGAAGATA AACTAAAACG CGAAGAAGAA 
AAAAGAGAAG CTGAAAAAAG TGAAGATTCC TCAGGTACTA CAGGCCTCTC AGGCTTACAT 
CGTACATACA GCCAGGACTG CAGCTTTAAG AACAGCATGT ATCATGTCGG AGATTATGTC 
TATGTTGAAC CTGCGGAGGC CAATCTACAA CCACATATAG TGTGTATTGA GAGACTGTGG 
GAGGATTCAG CTGGTGAAAA ATGGTTGTAC GGCTGTTGGT TTTATCGGCC AAATGAAACA 
TTCCATTTGG CTACACGAAA ATTTCTAGAA AAAGAAGTTT TTAAGAGTGA CTACTACAAT 
AAAGTACCTG TTAGTAAAAT TCTAGGCAAA TGTGTAGTCA TGTTTGTCAA GGAATACTTT 
AAATTATGTC CAGAAAACTT TCGCGATGAG GATGTTTTTG TCTGTGAATC GAGGTATTCT 
GCCAAAACCA AATCTTTTAA GAAAATTAAA CTGTGGACCA TGCCCATCAG TTCAGTTAGA 
TTTGTCCCTC GGGATGTGCC TTTGCCTGTG GTCCGAGTGG CCTCTGTGTT TGCAAATGCA 
GATAAAGGGG ATGATGAGAA GAATACAGAC AACTCAGATG ACAATAGAGC TGAAGACAAT 
TTTAACTTGG AAAAGGAAAA AGAAGATGTT CCTGTGGAGA TGTCCAATGG TGAGCCAGGT 
TGCCACTACT TTGAGCAGCT TCGGTACAAT GACATGTGGC TGAAGGTTGG TGATTGTGTC 
TTCATCAAAT CCCACGGCTT GGTGCGCCCT CGTGTGGGCA GAATTGAGAA AGTATGGGTC 
CGAGATGGAG CTGCATATTT TTATGGCCCT ATCTTCATTC ATCCAGAAGA AACAGAACAT 
GAGCCCACAA AAATGTTCTA CAAAAAAGAA GTGTTTCTGA GTAATCTGGA AGAGACCTGC 
CCTATGAGTT GTATTCTGGG GAAATGTGCA GTGCTGTCAT TCAAGGACTT CCTCTCCTGC 
AGGCCAACTG AAATACCAGA AAATGACATT CTGCTTTGTG AGAGCCGCTA TAATGAGAGT 
GACAAGCAGA TGAAGAAGTT CAAGGGTTTG AAGAGGTTTT CACTCTCTGC TAAAGTTGTA 
GATGATGAAA TCTACTACTT CAGAAAACCA ATCATTCCTC AGAAGGAACC CTCACCTTTG 
TTAGAAAAGA AGATACAATT GCTAGAAGCT AAATTTGCAG AGTTAGAAGG AGGAGATGAT 
GATATTGAGG AGATGGGAGA AGAGGATAGT GAAGTCATTG AAGCTCCATC TCTACCTCAA 
CTGCAGACAC CCCTGGCCAA TGAGTTGGAC CTCATGCCCT ATACACCCCC ACAGTCTACC 
CCAAAGTCTG CCAAAGGCAG TGCAAAGAAG GAAAGTTCTA AACGAAAAAT CAACATGAGT 
GGCTACATTT TGTTCAGCAG TGAAATGAGA GCTGTGATTA AAGCCCAGCA CCCAGACTAC 
TCTTTTGGGG AGCTCAGCAG ACTGGTGGGG ACAGAATGGA GAAACCTTGA AACAGCCAAG 
AAAGCAGAAT ATGAAGGTGT GATGAATCAA GGAGTAGCCC CCATGGTAGG GACTCCAGCA 
CCAGGTGGAA GTCCGTATGG ACAACAGGTA GGAGTTTTGG GACCTCCAGG GCAGCAGGCA 
CCACCTCCAT ATCCTGGTCC TCATCCAGCT GGCCCCCCTG TCATACAGCA GCCAACAACG 
CCCATGTTTG TGGCTCCCCC ACCAAAGACC CAAAGGCTTC TCCACTCAGA GGCCTACCTG 
AAATACATTG AAGGACTCAG TGCTGAATCC AACAGCATTA GCAAGTGGGA CCAAACTTTG 
GCAGCTCGAA GACGGGATGT CCATTTGTCC AAAGAACAGG AGAGCCGCCT ACCTTCTCAC 
TGGCTCAAAA GTAAAGGGGC ACACACCACC ATGGCAGATG CCCTCTGGCG CCTACGGGAT 
TTAATGCTTC GAGACACTCT CAACATCCGA CAGGCATACA ACCTAGAAAA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbrm1 ( XM_006519427.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519490.1 Mus musculus polybromo 1 (Pbrm1), transcript variant X48, mRNA.

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CloneID	OMu50273
Clone ID Related Accession (Same CDS sequence)	XM_006519427.3 , NM_001359856.1
Accession Version	XM_006519427.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein polybromo-1 isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519427.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GGTGTGATGA ATCAAGGAGT AGCCCCCATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAAC AGGTAGGAGT TTTGGGACCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGTCCTCATC CAGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACGCCCAT GTTTGTGGCT CCCCCACCAA AGACCCAAAG GCTTCTCCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCTG AATCCAACAG CATTAGCAAG 
TGGGACCAAA CTTTGGCAGC TCGAAGACGG GATGTCCATT TGTCCAAAGA ACAGGAGAGC 
CGCCTACCTT CTCACTGGCT CAAAAGTAAA GGGGCACACA CCACCATGGC AGATGCCCTC 
TGGCGCCTAC GGGATTTAAT GCTTCGAGAC ACTCTCAACA TCCGACAGGC ATACAACCTA 
GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519490.1
CDS	359..5170
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDIIRKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV

Target ORF information:

RefSeq Version	XM_006519427.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polybromo 1 (Pbrm1), transcript variant X48, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519427.3

ORF Insert Sequence:

ATGAGGAGAT TGGCTTTTCG AGGCGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCCACC TCTCCTTCCA GCAGTGTCAG TGGAGACTTT 
GATGACGGGC ACCATTCTGT GCCTACACCA GGCCCAAGCA GGAAAAGGAG AAGACTGTCC 
AATCTTCCAA CTGTAGATCC TATTGCTGTG TGCCATGAAC TCTATAACAC CATCCGAGAC 
TATAAGGATG AACAGGGCAG ACTCCTCTGT GAGCTGTTCA TTAGGGCTCC AAAGCGGAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTTAAAA TGGAAGAGTA TGATGATGTT AATCTACTGA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA CTATAAGCCA GATTCCCCTG AGTATAAAGC TGCTTGTAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAAGGAGA AGCAGACGAT 
GAAGATGATG ACGAAGATGG GCAAGACAAT CAAGGCACAC TGGCTGACGG CTCTTCTCCA 
GGTTATCTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGGC TCATCAGTGA ACTTTTTCAG AAACTGCCTT CCAAAGTGCA ATATCCAGAC 
TATTATGCAA TAATTAAGGA ACCTATAGAT CTCAAGACCA TTGCTCAGAG GATACAGAAT 
GGAAGCTACA AAAGTATACA CGCAATGGCC AAAGATATAG ATCTTCTAGC AAAAAATGCC 
AAAACATACA ATGAGCCTGG GTCTCAAGTA TTCAAGGATG CCAATTCGAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT CATGAAATGA CTAAATCAAG TCTTCGAATA 
AGGACTGCAT CAAATTTGGC TGCAGCCAGG CTGACAGGTC CTTCGCACAA TAAAAGCAGC 
CTTGGTGAAG AAAGAAACCC CACTAGCAAG TATTACCGTA ATAAAAGAGC AGTCCAAGGG 
GGTCGCTTGT CAGCAATTAC CATGGCACTT CAGTATGGAT CAGAGAGTGA AGAGGACGCT 
GCTTTAGCTG CTGCACGCTA TGAAGAAGGG GAATCTGAAG CAGAGAGCAT CACTTCCTTC 
ATGGACGTTT CCAACCCCTT TCATCAGCTT TACGACACAG TTAGGAGCTG TAGGAATCAC 
CAAGGGCAGC TCATAGCTGA ACCTTTCTTC CATTTGCCTT CAAAGAAAAA ATACCCAGAT 
TATTATCAGC AAATTAAAAT GCCCATATCA CTTCAACAGA TCAGAACAAA GCTAAAGAAC 
CAAGAATATG AAACTTTAGA TCATTTGGAG TGTGATCTGA ATTTAATGTT TGAAAATGCC 
AAACGTTATA ACGTTCCCAA TTCAGCCATC TATAAGCGAG TTCTAAAACT GCAGCAAGTC 
ATGCAGGCAA AGAAGAAGGA GCTTGCGAGG AGAGATGACA TTGAGGACGG AGACAGCATG 
ATCTCCTCAG CCACTTCTGA CACTGGTAGT GCCAAAAGGA AAAGTAAAAA GAACATAAGA 
AAACAGCGAA TGAAAATTTT ATTCAATGTT GTTCTTGAAG CTCGAGAGCC AGGTTCAGGC 
AGAAGACTTT GCGATCTATT TATGGTTAAG CCATCCAAGA AGGACTATCC TGATTATTAT 
AAAATCATCT TAGAGCCAAT GGACCTGAAA ATAATTGAGC ATAACATCCG AAATGACAAA 
TATGCAGGTG AAGAAGGAAT GATGGAAGAC ATGAAACTCA TGTTCCGCAA TGCCAGGCAC 
TACAATGAGG AGGGCTCCCA GGTATACAAT GATGCCCATA TCCTGGAGAA GTTACTCAAA 
GATAAAAGGA AAGAGCTGGG CCCTCTGCCT GATGATGATG ACATGGCTTC TCCCAAACTT 
AAATTGAGTA GGAAGAGTGG TGTTTCTCCT AAGAAATCAA AGTACATGAC TCCAATGCAG 
CAGAAACTGA ATGAAGTGTA TGAAGCTGTA AAGAACTATA CTGATAAGAG GGGTCGCCGC 
CTTAGTGCTA TATTTCTAAG ACTCCCCTCT AGATCAGAGC TGCCTGACTA CTACCTGACC 
ATTAAAAAGC CCATGGACAT GGAAAAAATT CGAAGTCACA TGATGGCAAA CAAGTACCAA 
GACATAGATT CTATGGTAGA GGACTTTGTC ATGATGTTTA ATAATGCCTG TACCTACAAT 
GAACCAGAGT CTTTGATCTA CAAAGATGCC CTTGTACTGC ATAAAGTCCT CCTTGAGACT 
CGGAGAGACC TGGAGGGAGA TGAGGATTCT CATGTCCCTA ATGTGACGTT GCTGATTCAA 
GAGCTCATCC ATAACCTTTT TGTGTCAGTC ATGAGTCATC AGGATGACGA AGGGAGGTGT 
TACAGCGACT CCTTAGCAGA AATTCCTGCT GTGGATCCCA ACTCTCCCAA TAAACCTCCC 
CTTACATTTG ACATTATCAG GAAAAATGTT GAAAGTAATC GGTATCGGCG ACTTGATTTA 
TTTCAGGAGC ATATGTTTGA AGTATTGGAA CGGGCAAGAA GGATGAACCG GACAGATTCC 
GAAATATATG AGGATGCTGT AGAACTTCAG CAGTTTTTTA TTAGAATTCG TGATGAACTC 
TGCAAAAATG GAGAGATCCT TCTTTCTCCA GCACTCAGCT ATACCACAAA ACACTTGCAT 
AACGATGTGG AAAAAGAAAA AAAGGAAAAA TTGCCTAAAG AAATAGAGGA AGATAAACTA 
AAACGCGAAG AAGAAAAAAG AGAAGCTGAA AAAAGTGAAG ATTCCTCAGG TACTACAGGC 
CTCTCAGGCT TACATCGTAC ATACAGCCAG GACTGCAGCT TTAAGAACAG CATGTATCAT 
GTCGGAGATT ATGTCTATGT TGAACCTGCG GAGGCCAATC TACAACCACA TATAGTGTGT 
ATTGAGAGAC TGTGGGAGGA TTCAGCTGGT GAAAAATGGT TGTACGGCTG TTGGTTTTAT 
CGGCCAAATG AAACATTCCA TTTGGCTACA CGAAAATTTC TAGAAAAAGA AGTTTTTAAG 
AGTGACTACT ACAATAAAGT ACCTGTTAGT AAAATTCTAG GCAAATGTGT AGTCATGTTT 
GTCAAGGAAT ACTTTAAATT ATGTCCAGAA AACTTTCGCG ATGAGGATGT TTTTGTCTGT 
GAATCGAGGT ATTCTGCCAA AACCAAATCT TTTAAGAAAA TTAAACTGTG GACCATGCCC 
ATCAGTTCAG TTAGATTTGT CCCTCGGGAT GTGCCTTTGC CTGTGGTCCG AGTGGCCTCT 
GTGTTTGCAA ATGCAGATAA AGGGGATGAT GAGAAGAATA CAGACAACTC AGATGACAAT 
AGAGCTGAAG ACAATTTTAA CTTGGAAAAG GAAAAAGAAG ATGTTCCTGT GGAGATGTCC 
AATGGTGAGC CAGGTTGCCA CTACTTTGAG CAGCTTCGGT ACAATGACAT GTGGCTGAAG 
GTTGGTGATT GTGTCTTCAT CAAATCCCAC GGCTTGGTGC GCCCTCGTGT GGGCAGAATT 
GAGAAAGTAT GGGTCCGAGA TGGAGCTGCA TATTTTTATG GCCCTATCTT CATTCATCCA 
GAAGAAACAG AACATGAGCC CACAAAAATG TTCTACAAAA AAGAAGTGTT TCTGAGTAAT 
CTGGAAGAGA CCTGCCCTAT GAGTTGTATT CTGGGGAAAT GTGCAGTGCT GTCATTCAAG 
GACTTCCTCT CCTGCAGGCC AACTGAAATA CCAGAAAATG ACATTCTGCT TTGTGAGAGC 
CGCTATAATG AGAGTGACAA GCAGATGAAG AAGTTCAAGG GTTTGAAGAG GTTTTCACTC 
TCTGCTAAAG TTGTAGATGA TGAAATCTAC TACTTCAGAA AACCAATCAT TCCTCAGAAG 
GAACCCTCAC CTTTGTTAGA AAAGAAGATA CAATTGCTAG AAGCTAAATT TGCAGAGTTA 
GAAGGAGGAG ATGATGATAT TGAGGAGATG GGAGAAGAGG ATAGTGAAGT CATTGAAGCT 
CCATCTCTAC CTCAACTGCA GACACCCCTG GCCAATGAGT TGGACCTCAT GCCCTATACA 
CCCCCACAGT CTACCCCAAA GTCTGCCAAA GGCAGTGCAA AGAAGGAAAG TTCTAAACGA 
AAAATCAACA TGAGTGGCTA CATTTTGTTC AGCAGTGAAA TGAGAGCTGT GATTAAAGCC 
CAGCACCCAG ACTACTCTTT TGGGGAGCTC AGCAGACTGG TGGGGACAGA ATGGAGAAAC 
CTTGAAACAG CCAAGAAAGC AGAATATGAA GGTGTGATGA ATCAAGGAGT AGCCCCCATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAAC AGGTAGGAGT TTTGGGACCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGTCCTCATC CAGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACGCCCAT GTTTGTGGCT CCCCCACCAA AGACCCAAAG GCTTCTCCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCTG AATCCAACAG CATTAGCAAG 
TGGGACCAAA CTTTGGCAGC TCGAAGACGG GATGTCCATT TGTCCAAAGA ACAGGAGAGC 
CGCCTACCTT CTCACTGGCT CAAAAGTAAA GGGGCACACA CCACCATGGC AGATGCCCTC 
TGGCGCCTAC GGGATTTAAT GCTTCGAGAC ACTCTCAACA TCCGACAGGC ATACAACCTA 
GAAAATGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of PBRM1 rescues VHL dependent replication stress to promote renal carcinogenesis.
Nature communications8(1)2026(2017 Dec)
Espana-Agusti J,Warren A,Chew SK,Adams DJ,Matakidou A

Cancer discovery7(8)802-804(2017 08)
Leung JY,Kim WY

Modeling Renal Cell Carcinoma in Mice:
Cancer discovery7(8)900-917(2017 08)
Gu YF,Cohn S,Christie A,McKenzie T,Wolff N,Do QN,Madhuranthakam AJ,Pedrosa I,Wang T,Dey A,Busslinger M,Xie XJ,Hammer RE,McKay RM,Kapur P,Brugarolas J

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Pbrm1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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