Nelfb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nelfb
Entrez Gene ID	58202
Full Name	negative elongation factor complex member B
Synonyms	A730008L03Rik,AB041607,AI663983,Cobra1,Nelf-b
General protein information	Preferred Names negative elongation factor complex member B Names negative elongation factor B cofactor of BRCA1 negative elongation factor complex member B, Cobra1 ortholog of human cofactor of BRCA1 (COBRA1)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A3
Summary	This gene encodes subunit B of a metazoan-specific, four-subunit protein complex that regulates promoter-proximal pausing of RNA polymerase II. RNA polymerase II pausing is thought to be important for coordination of gene transcription during embryonic development and stress responses. Consistently, disruption of this gene in mouse causes inner cell mass deficiency and embryonic lethality. In addition, this gene is required for maintenance of mouse embryonic stem cells by preventing expression of developmental genes. In adult mice, conditional deletion of this gene results in cardiomyopathy and impaired response to cardiac stress. Multiple protein isoforms are encoded through the use of a non-AUG (CUG) initiation codon and an alternative downstream AUG initiation codon. In addition, alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001310157.1	NP_001297086.1	negative elongation factor B isoform 2
NM_021393.3	NP_067368.3	negative elongation factor B isoform 1

Pathways from BioSystems

Homologies

	COBRA1	XP_001087434.1
Rattus norvegicus (Norway rat)	Nelfb	NP_001101287.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012109	XP_320419.3
Homo sapiens (human)	NELFB	NP_056271.2
Bos taurus (cattle)	NELFB	XP_002691742.3
Xenopus tropicalis (tropical clawed frog)	nelfb	NP_001011448.1
Mus musculus (house mouse)	Nelfb	NP_067368.2
Gallus gallus (chicken)	COBRA1	NP_001006202.1
Pan troglodytes (chimpanzee)	COBRA1	XP_001136264.3
Canis lupus familiaris (dog)	NELFB	XP_548351.2
Danio rerio (zebrafish)	nelfb	NP_956462.1
Drosophila melanogaster (fruit fly)	NELF-B	NP_572402.1

Gene Ontology
Bibliography

Related articles in PubMed

Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis.
Zhang X, Chiang HC, Wang Y, Zhang C, Smith S, Zhao X, Nair SJ, Michalek J, Jatoi I, Lautner M, Oliver B, Wang H, Petit A, Soler T, Brunet J, Mateo F, Angel Pujana M, Poggi E, Chaldekas K, Isaacs C, Peshkin BN, Ochoa O, Chedin F, Theoharis C, Sun LZ, Curiel TJ, Elledge R, Jin VX, Hu Y, Li R
Nature communications815908(2017 Jun)

Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.
Pan H, Zhao X, Zhang X, Abouelsoud M, Sun J, April C, Amleh A, Fan JB, Hu Y, Li R
PloS one10(5)e0127422(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study uncovers a previously unappreciated, DNA repair-independent function of BRCA1 in antagonizing COBRA1-dependent transcription program during mammary gland development.
Title: Genetic suppression reveals DNA repair-independent antagonism between BRCA1 and COBRA1 in mammary gland development.

Human and mouse Nelfb encode proteins translated from a non-AUG (CUG) start codon. The majority of mouse tissues examined express the isoform translated from the CUG start codon, but a small number of tissues also express a shorter isoform of the same apparent size as the AUG-initiated isoform.
Title: Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.

Cobra1 Is Required for TrkC-Induced Apoptosis.
Title: The dependence receptor TrkC triggers mitochondria-dependent apoptosis upon Cobra-1 recruitment.

genetic and genomic studies provide new physiological and molecular insight into Nelf-mediated pol II pausing.
Title: Genetic and genomic analyses of RNA polymerase II-pausing factor in regulation of mammalian transcription and cell growth.

findings also indicate that Cobra1 helps maintain the undifferentiated state of mESCs by preventing unscheduled expression of developmental genes
Title: Mouse cofactor of BRCA1 (Cobra1) is required for early embryogenesis.

The following Nelfb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nelfb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu69774	NM_021393.3 Latest version!	Mus musculus negative elongation factor complex member B (Nelfb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OMu69773	NM_001310157.1 Latest version!	Mus musculus negative elongation factor complex member B (Nelfb), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nelfb ( NM_021393.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067368.3 Mus musculus negative elongation factor complex member B (Nelfb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu69774
Clone ID Related Accession (Same CDS sequence)	NM_021393.3
Accession Version	NM_021393.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	negative elongation factor B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL732309.9, CJ100279.1, AK173124.1 and AI843129.1. On Jul 3, 2015 this sequence version replaced NM_021393.2. Transcript Variant: This variant (1) represents the longer isoform (1), which is translated using a non-AUG (CUG) start codon. CCDS Note: The coding region has been updated to extend the protein N-terminus and to use a non-AUG (CUG) start codon, supported by data in PMID:26010750. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC004762.1, SRR1660813.236916.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 26010750 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGAGGCGG CGGGCGAACG GGGCTTGGGG GGTCCTCGTG GCACCGTGGA GAGGGCTTCT 
GGGGCTCCGT CAGGGTCCGC TACGGCTCCA GCGGAGCGCG GTGGGGATGG GGTACACAGC 
CGAGCGTCCG CCGGAGCCTC CGCCATGTTC GCGGGGCTCC AGGACCTGGG CGTGGCCAAT 
GGCGAGGACC TGAAAGAGAC CCTGACCAAC TGCACCGAGC CTCTCAAGGC CATTGAACAA 
TTTCAGACAG AGAATGGCGT GTTGCTGCCT TCCCTGCAGT CGGCCCTGCC CTTCTTGGAT 
CTACACGGGA CACCGCGGCT GGAGTTCCAT CAGTCAGTGT TTGATGAACT ACGTGACAAA 
CTTCTGGAAC GAGTGTCAGC AATTGCCTCT GAGGGAAAGG CAGAGGAGAG GTACAAGAAG 
CTGGAAGACC TTCTGGAAAA GAGCTTTTCT TTGGTCAAGA TGCCATCCCT ACAACCAGTG 
GTGATGTGTG TTATGAAACA CTTGCCCAAG GTTCCTGAGA AGAAGCTGAA GCTGGTGATG 
GCTGACAAGG AGTTGTACCG GGCATGTGCT GTGGAAGTGA AGAGGCAGAT TTGGCAAGAC 
AACCAGGCTC TCTTTGGTGA TGAAGTCTCA CCACTGCTGA AGCAGTATAT CCTAGAGAAA 
GAGAGTGCGC TCTTCAGCAC AGAGCTCTCT GTGCTGCACA ACTTCTTCAG CCCTTCCCCC 
AAGACCCGGC GCCAAGGAGA GGTGGTGCAG AAGCTGACGC AGATGGTAGG GAAGAATGTG 
AAGCTGTACG ACATGGTGCT GCAGTTCCTG CGCACACTGT TCCTGCGGAC CAGGAATGTG 
CACTACTGCA CGTTACGAGC TGAGCTGCTC ATGTCTTTGC ATGACTTGGA CGTCAGTGAC 
ATCTGCACTG TGGATCCCTG CCATAAGTTC ACCTGGTGCC TGGATGCCTG CATCCGGGAA 
CGGTTTGTGG ACAGCAAAAG AGCCCGGGAG CTTCAGGGAT TTCTTGATGG TGTGAAGAAG 
GGCCAGGAAC AAGTTTTGGG GGATTTGTCT ATGATCCTGT GCGACCCCTT TGCCATCAAC 
ACACTGTCCC TGAGCACCAT CAGGCACTTG CAGGAGCTGG TTAGCCAGGA GACACTGCCC 
AGGGATAGTC CCGACCTCCT CCTACTACTT AGGCTGCTGG CACTGGGCCA AGGTGCATGG 
GACTTAATAG ATAGCCAGGT CTTCAAGGAG CCTAAGATGG AGGCAGAGCT CATCACCAAG 
TTCCTGCCGA TGCTCATGTC CCTCGTGGTG GATGACTTCA CTTTCAACGT AGATCAGAAG 
CTTCCAGCTG AGGAGAAAGC CTCAGTCACA TACCCCAACA CACTTCCTGA GAGTTTCACC 
AAGTTTCTGC AGGAGCAGCG CATGGCTTGT GAGGTGGGAC TATACTACGT CCTACACATC 
ACCAAGCAGA GAAACAAGAA TGCTCTCCTG CGCCTGCTGC CTGGACTTGT GGAAACGTTT 
GGTGACCTGG CCTTCAGTGA CATCTTTCTC CACCTGCTCA CTGGGAGCCT GGTACTGCTT 
GCTGATGAAT TTGCCCTGGA GGATTTCTGC AGCAGCCTCT TTGATGGCTT CTTCCTCACT 
GCCTCTCCCA GGAAGGAGAA TGTGCACCGT CATGTGCTGA GGCTTCTCCT CCACTTGCAT 
GCAAGAGTGG CCCCTTCCAA GCTGGAGGCA TTGCAGAAGG CTCTGGAGCC CACAGGCCAG 
AGCGGGGAGG CCGTGAAGGA GCTCTACTCC CAGCTTGGTG AGAAGTTGGA GCAGTTAGAC 
CATCGAAAGC CCAGTCCTAC CCAAGCTGCA GAGACACCAG CACTGGATCT GCCTCTTCCC 
AGCGTGCCTG CTCCAGCCAC ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067368.3
CDS	143..2029
Translation	LEAAGERGLGGPRGTVERASGAPSGSATAPAERGGDGVHSRASAGASAMFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQKLTQMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVSDICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLSLSTIRHLQELVSQETLPRDSPDLLLLLRLLALGQGAWDLIDSQVFKEPKMEAELITKFLPMLMSLVVDDFTFNVDQKLPAEEKASVTYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFSDIFLHLLTGSLVLLADEFALEDFCSSLFDGFFLTASPRKENVHRHVLRLLLHLHARVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPTQAAETPALDLPLPSVPAPATL

Target ORF information:

RefSeq Version	NM_021393.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus negative elongation factor complex member B (Nelfb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021393.3

ORF Insert Sequence:

CTGGAGGCGG CGGGCGAACG GGGCTTGGGG GGTCCTCGTG GCACCGTGGA GAGGGCTTCT 
GGGGCTCCGT CAGGGTCCGC TACGGCTCCA GCGGAGCGCG GTGGGGATGG GGTACACAGC 
CGAGCGTCCG CCGGAGCCTC CGCCATGTTC GCGGGGCTCC AGGACCTGGG CGTGGCCAAT 
GGCGAGGACC TGAAAGAGAC CCTGACCAAC TGCACCGAGC CTCTCAAGGC CATTGAACAA 
TTTCAGACAG AGAATGGCGT GTTGCTGCCT TCCCTGCAGT CGGCCCTGCC CTTCTTGGAT 
CTACACGGGA CACCGCGGCT GGAGTTCCAT CAGTCAGTGT TTGATGAACT ACGTGACAAA 
CTTCTGGAAC GAGTGTCAGC AATTGCCTCT GAGGGAAAGG CAGAGGAGAG GTACAAGAAG 
CTGGAAGACC TTCTGGAAAA GAGCTTTTCT TTGGTCAAGA TGCCATCCCT ACAACCAGTG 
GTGATGTGTG TTATGAAACA CTTGCCCAAG GTTCCTGAGA AGAAGCTGAA GCTGGTGATG 
GCTGACAAGG AGTTGTACCG GGCATGTGCT GTGGAAGTGA AGAGGCAGAT TTGGCAAGAC 
AACCAGGCTC TCTTTGGTGA TGAAGTCTCA CCACTGCTGA AGCAGTATAT CCTAGAGAAA 
GAGAGTGCGC TCTTCAGCAC AGAGCTCTCT GTGCTGCACA ACTTCTTCAG CCCTTCCCCC 
AAGACCCGGC GCCAAGGAGA GGTGGTGCAG AAGCTGACGC AGATGGTAGG GAAGAATGTG 
AAGCTGTACG ACATGGTGCT GCAGTTCCTG CGCACACTGT TCCTGCGGAC CAGGAATGTG 
CACTACTGCA CGTTACGAGC TGAGCTGCTC ATGTCTTTGC ATGACTTGGA CGTCAGTGAC 
ATCTGCACTG TGGATCCCTG CCATAAGTTC ACCTGGTGCC TGGATGCCTG CATCCGGGAA 
CGGTTTGTGG ACAGCAAAAG AGCCCGGGAG CTTCAGGGAT TTCTTGATGG TGTGAAGAAG 
GGCCAGGAAC AAGTTTTGGG GGATTTGTCT ATGATCCTGT GCGACCCCTT TGCCATCAAC 
ACACTGTCCC TGAGCACCAT CAGGCACTTG CAGGAGCTGG TTAGCCAGGA GACACTGCCC 
AGGGATAGTC CCGACCTCCT CCTACTACTT AGGCTGCTGG CACTGGGCCA AGGTGCATGG 
GACTTAATAG ATAGCCAGGT CTTCAAGGAG CCTAAGATGG AGGCAGAGCT CATCACCAAG 
TTCCTGCCGA TGCTCATGTC CCTCGTGGTG GATGACTTCA CTTTCAACGT AGATCAGAAG 
CTTCCAGCTG AGGAGAAAGC CTCAGTCACA TACCCCAACA CACTTCCTGA GAGTTTCACC 
AAGTTTCTGC AGGAGCAGCG CATGGCTTGT GAGGTGGGAC TATACTACGT CCTACACATC 
ACCAAGCAGA GAAACAAGAA TGCTCTCCTG CGCCTGCTGC CTGGACTTGT GGAAACGTTT 
GGTGACCTGG CCTTCAGTGA CATCTTTCTC CACCTGCTCA CTGGGAGCCT GGTACTGCTT 
GCTGATGAAT TTGCCCTGGA GGATTTCTGC AGCAGCCTCT TTGATGGCTT CTTCCTCACT 
GCCTCTCCCA GGAAGGAGAA TGTGCACCGT CATGTGCTGA GGCTTCTCCT CCACTTGCAT 
GCAAGAGTGG CCCCTTCCAA GCTGGAGGCA TTGCAGAAGG CTCTGGAGCC CACAGGCCAG 
AGCGGGGAGG CCGTGAAGGA GCTCTACTCC CAGCTTGGTG AGAAGTTGGA GCAGTTAGAC 
CATCGAAAGC CCAGTCCTAC CCAAGCTGCA GAGACACCAG CACTGGATCT GCCTCTTCCC 
AGCGTGCCTG CTCCAGCCAC ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nelfb ( NM_001310157.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297086.1 Mus musculus negative elongation factor complex member B (Nelfb), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69773
Clone ID Related Accession (Same CDS sequence)	NM_001310157.1
Accession Version	NM_001310157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	negative elongation factor B isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL732309.9, CJ100279.1, CX241076.1, AK173124.1 and AI843129.1. Transcript Variant: This variant (2) differs in the 5' UTR and uses alternate splice sites in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB041607.1, SRR1660817.230045.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCATCCC TACAACCAGT GGTGATGTGT GTTATGAAAC ACTTGCCCAA GGTTCCTGAG 
AAGAAGCTGA AGCTGGTGAT GGCTGACAAG GAGTTGTACC GGGCATGTGC TGTGGAAGTG 
AAGAGGCAGA TTTGGCAAGA CAACCAGGCT CTCTTTGGTG ATGAAGTCTC ACCACTGCTG 
AAGCAGTATA TCCTAGAGAA AGAGAGTGCG CTCTTCAGCA CAGAGCTCTC TGTGCTGCAC 
AACTTCTTCA GCCCTTCCCC CAAGACCCGG CGCCAAGGAG AGGTGGTGCA GAAGCTGACG 
CAGATGGTAG GGAAGAATGT GAAGCTGTAC GACATGGTGC TGCAGTTCCT GCGCACACTG 
TTCCTGCGGA CCAGGAATGT GCACTACTGC ACGTTACGAG CTGAGCTGCT CATGTCTTTG 
CATGACTTGG ACGTCAGTGA CATCTGCACT GTGGATCCCT GCCATAAGTT CACCTGGTGC 
CTGGATGCCT GCATCCGGGA ACGGTTTGTG GACAGCAAAA GAGCCCGGGA GCTTCAGGGA 
TTTCTTGATG GTGTGAAGAA GGGCCAGGAA CAAGTTTTGG GGGATTTGTC TATGATCCTG 
TGCGACCCCT TTGCCATCAA CACACTGTCC CTGAGCACCA TCAGGCACTT GCAGGAGCTG 
GTTAGCCAGG AGACACTGCC CAGGGATAGT CCCGACCTCC TCCTACTACT TAGGCTGCTG 
GCACTGGGCC AAGGTGCATG GGACTTAATA GATAGCCAGG TCTTCAAGGA GCCTAAGATG 
GAGGCAGAGC TCATCACCAA GTTCCTGCCG ATGCTCATGT CCCTCGTGGT GGATGACTTC 
ACTTTCAACG TAGATCAGAA GCTTCCAGCT GAGGAGAAAG CCTCAGTCAC ATACCCCAAC 
ACACTTCCTG AGAGTTTCAC CAAGTTTCTG CAGGAGCAGC GCATGGCTTG TGAGGTGGGA 
CTATACTACG TCCTACACAT CACCAAGCAG AGAAACAAGA ATGCTCTCCT GCGCCTGCTG 
CCTGGACTTG TGGAAACGTT TGGTGACCTG GCCTTCAGTG ACATCTTTCT CCACCTGCTC 
ACTGGGAGCC TGGTACTGCT TGCTGATGAA TTTGCCCTGG AGGATTTCTG CAGCAGCCTC 
TTTGATGGCT TCTTCCTCAC TGCCTCTCCC AGGAAGGAGA ATGTGCACCG TCATGTGCTG 
AGGCTTCTCC TCCACTTGCA TGCAAGAGTG GCCCCTTCCA AGCTGGAGGC ATTGCAGAAG 
GCTCTGGAGC CCACAGGCCA GAGCGGGGAG GCCGTGAAGG AGCTCTACTC CCAGCTTGGT 
GAGAAGTTGG AGCAGTTAGA CCATCGAAAG CCCAGTCCTA CCCAAGCTGC AGAGACACCA 
GCACTGGATC TGCCTCTTCC CAGCGTGCCT GCTCCAGCCA CACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297086.1
CDS	831..2258
Translation	MPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQKLTQMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVSDICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLSLSTIRHLQELVSQETLPRDSPDLLLLLRLLALGQGAWDLIDSQVFKEPKMEAELITKFLPMLMSLVVDDFTFNVDQKLPAEEKASVTYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFSDIFLHLLTGSLVLLADEFALEDFCSSLFDGFFLTASPRKENVHRHVLRLLLHLHARVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPTQAAETPALDLPLPSVPAPATL

Target ORF information:

RefSeq Version	NM_001310157.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus negative elongation factor complex member B (Nelfb), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310157.1

ORF Insert Sequence:

ATGCCATCCC TACAACCAGT GGTGATGTGT GTTATGAAAC ACTTGCCCAA GGTTCCTGAG 
AAGAAGCTGA AGCTGGTGAT GGCTGACAAG GAGTTGTACC GGGCATGTGC TGTGGAAGTG 
AAGAGGCAGA TTTGGCAAGA CAACCAGGCT CTCTTTGGTG ATGAAGTCTC ACCACTGCTG 
AAGCAGTATA TCCTAGAGAA AGAGAGTGCG CTCTTCAGCA CAGAGCTCTC TGTGCTGCAC 
AACTTCTTCA GCCCTTCCCC CAAGACCCGG CGCCAAGGAG AGGTGGTGCA GAAGCTGACG 
CAGATGGTAG GGAAGAATGT GAAGCTGTAC GACATGGTGC TGCAGTTCCT GCGCACACTG 
TTCCTGCGGA CCAGGAATGT GCACTACTGC ACGTTACGAG CTGAGCTGCT CATGTCTTTG 
CATGACTTGG ACGTCAGTGA CATCTGCACT GTGGATCCCT GCCATAAGTT CACCTGGTGC 
CTGGATGCCT GCATCCGGGA ACGGTTTGTG GACAGCAAAA GAGCCCGGGA GCTTCAGGGA 
TTTCTTGATG GTGTGAAGAA GGGCCAGGAA CAAGTTTTGG GGGATTTGTC TATGATCCTG 
TGCGACCCCT TTGCCATCAA CACACTGTCC CTGAGCACCA TCAGGCACTT GCAGGAGCTG 
GTTAGCCAGG AGACACTGCC CAGGGATAGT CCCGACCTCC TCCTACTACT TAGGCTGCTG 
GCACTGGGCC AAGGTGCATG GGACTTAATA GATAGCCAGG TCTTCAAGGA GCCTAAGATG 
GAGGCAGAGC TCATCACCAA GTTCCTGCCG ATGCTCATGT CCCTCGTGGT GGATGACTTC 
ACTTTCAACG TAGATCAGAA GCTTCCAGCT GAGGAGAAAG CCTCAGTCAC ATACCCCAAC 
ACACTTCCTG AGAGTTTCAC CAAGTTTCTG CAGGAGCAGC GCATGGCTTG TGAGGTGGGA 
CTATACTACG TCCTACACAT CACCAAGCAG AGAAACAAGA ATGCTCTCCT GCGCCTGCTG 
CCTGGACTTG TGGAAACGTT TGGTGACCTG GCCTTCAGTG ACATCTTTCT CCACCTGCTC 
ACTGGGAGCC TGGTACTGCT TGCTGATGAA TTTGCCCTGG AGGATTTCTG CAGCAGCCTC 
TTTGATGGCT TCTTCCTCAC TGCCTCTCCC AGGAAGGAGA ATGTGCACCG TCATGTGCTG 
AGGCTTCTCC TCCACTTGCA TGCAAGAGTG GCCCCTTCCA AGCTGGAGGC ATTGCAGAAG 
GCTCTGGAGC CCACAGGCCA GAGCGGGGAG GCCGTGAAGG AGCTCTACTC CCAGCTTGGT 
GAGAAGTTGG AGCAGTTAGA CCATCGAAAG CCCAGTCCTA CCCAAGCTGC AGAGACACCA 
GCACTGGATC TGCCTCTTCC CAGCGTGCCT GCTCCAGCCA CACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis.
Nature communications815908(2017 Jun)
Zhang X,Chiang HC,Wang Y,Zhang C,Smith S,Zhao X,Nair SJ,Michalek J,Jatoi I,Lautner M,Oliver B,Wang H,Petit A,Soler T,Brunet J,Mateo F,Angel Pujana M,Poggi E,Chaldekas K,Isaacs C,Peshkin BN,Ochoa O,Chedin F,Theoharis C,Sun LZ,Curiel TJ,Elledge R,Jin VX,Hu Y,Li R

Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.
PloS one10(5)e0127422(2015)
Pan H,Zhao X,Zhang X,Abouelsoud M,Sun J,April C,Amleh A,Fan JB,Hu Y,Li R

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Nelfb cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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