NELFB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NELFB
Entrez Gene ID	25920
Full Name	negative elongation factor complex member B
Synonyms	COBRA1,NELF-B
General protein information	Preferred Names negative elongation factor complex member B Names negative elongation factor B cofactor of BRCA1 negative elongation factor protein B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.3
Summary	NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008].
Disorder MIM:	611180

mRNA and Protein(s)

mRNA	Protein	Name
NM_015456.4	NP_056271.3	negative elongation factor B
NM_015456.5	NP_056271.3	negative elongation factor B

Pathways from BioSystems

Homologies

	COBRA1	XP_001087434.1
Rattus norvegicus (Norway rat)	Nelfb	NP_001101287.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012109	XP_320419.3
Homo sapiens (human)	NELFB	NP_056271.2
Bos taurus (cattle)	NELFB	XP_002691742.3
Xenopus tropicalis (tropical clawed frog)	nelfb	NP_001011448.1
Mus musculus (house mouse)	Nelfb	NP_067368.2
Gallus gallus (chicken)	COBRA1	NP_001006202.1
Pan troglodytes (chimpanzee)	COBRA1	XP_001136264.3
Canis lupus familiaris (dog)	NELFB	XP_548351.2
Danio rerio (zebrafish)	nelfb	NP_956462.1
Drosophila melanogaster (fruit fly)	NELF-B	NP_572402.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Knockdown of COBRA1 decreases the proliferation and migration of hepatocellular carcinoma cells.
El Zeneini E, Kamel S, El-Meteini M, Amleh A
Oncology reports37(3)1896-1906(2017 Mar)

Architecture and RNA binding of the human negative elongation factor.
Vos SM, P?llmann D, Caizzi L, Hofmann KB, Rombaut P, Zimniak T, Herzog F, Cramer P
eLife5(2016 06)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High COBRA1 expression is associated with hepatocellular carcinoma.
Title: Knockdown of COBRA1 decreases the proliferation and migration of hepatocellular carcinoma cells.

A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo.
Title: Architecture and RNA binding of the human negative elongation factor.

both human and mouse NELF-B proteins are translated from a non-AUG codon upstream of the annotated AUG.
Title: Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.

These studies allow us to position the actions of two new modulators of GR-regulated transactivation, NELF-A and NELF-B, relative to other factors in the overall gene induction sequence.
Title: A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.

data show that NELF subunits exhibit highly specific subcellular localizations, such as in NELF bodies or in midbodies, and some shuttle actively between the nucleus and cytoplasm; loss of NELF from cells can lead to enlarged and/or multiple nuclei
Title: Cellular dynamics of the negative transcription elongation factor NELF.

The following NELFB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NELFB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu66637	NM_015456.5 Latest version!	Homo sapiens negative elongation factor complex member B (NELFB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
OHu66637	NM_015456.4	Homo sapiens negative elongation factor complex member B (NELFB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NELFB ( NM_015456.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056271.3 Homo sapiens negative elongation factor complex member B (NELFB), mRNA.

Price & Availability↑

CloneID	OHu66637
Clone ID Related Accession (Same CDS sequence)	NM_015456.4 , NM_015456.5
Accession Version	NM_015456.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Homo sapiens(human)
Product	negative elongation factor B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT003266.1, DA210427.1, BC019029.1, AK022651.1 and AL050280.1. On Jul 3, 2015 this sequence version replaced NM_015456.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.162629.1, AK091056.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 26010750 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGCCGAGC TGGAGGGCGC CGGGGAGCGG GGCTCGGGCG GTCCCCGAGG CCCGGCGGAG 
CGGGCTTCTG GGGTGTCTGC GGCGGCGCCG GGGGAACGGG CTGGGGATGG GGCGCCTAGC 
CGGGCGGTGG CCGGGGCCTC GGCCATGTTC GCGGGGCTGC AGGACCTGGG CGTGGCCAAC 
GGCGAGGACC TGAAGGAGAC CCTGACCAAC TGCACGGAGC CGCTCAAGGC CATCGAGCAG 
TTCCAGACAG AGAATGGTGT GCTGCTGCCA TCTCTTCAGT CAGCCCTCCC CTTCTTGGAC 
CTGCACGGGA CGCCGCGGCT GGAGTTCCAC CAGTCGGTAT TCGATGAGCT GCGGGACAAG 
CTGCTGGAGC GAGTGTCAGC CATCGCTTCG GAGGGGAAGG CTGAGGAAAG GTACAAGAAG 
CTGGAAGACC TTCTGGAGAA GAGCTTTTCT CTGGTGAAGA TGCCGTCCCT GCAGCCCGTG 
GTGATGTGCG TCATGAAGCA CCTGCCCAAG GTTCCGGAGA AAAAACTGAA GCTGGTTATG 
GCTGACAAGG AGCTGTATCG AGCCTGCGCC GTGGAGGTGA AGCGGCAGAT CTGGCAAGAC 
AACCAGGCCC TCTTCGGGGA CGAGGTTTCC CCACTCCTGA AGCAGTACAT CCTGGAGAAG 
GAGAGCGCTC TCTTCAGTAC AGAGCTCTCT GTCCTGCACA ACTTTTTCAG TCCTTCCCCC 
AAGACCAGGC GCCAGGGCGA GGTGGTGCAG CGGCTGACGC GGATGGTGGG GAAGAACGTG 
AAGCTGTACG ACATGGTGCT GCAGTTTCTG CGCACGCTCT TCCTGCGCAC GCGGAATGTG 
CACTACTGCA CGCTGCGGGC TGAGCTGCTC ATGTCCCTGC ACGACCTGGA CGTGGGTGAA 
ATCTGCACCG TGGACCCGTG CCACAAGTTC ACCTGGTGCC TGGACGCCTG CATCCGAGAG 
CGGTTCGTGG ACAGCAAGAG GGCGCGGGAG CTGCAGGGGT TTCTCGATGG CGTCAAGAAG 
GGCCAGGAGC AGGTGCTGGG GGACCTGTCC ATGATCCTGT GTGACCCCTT CGCCATCAAC 
ACGCTGGCAC TGAGCACAGT CAGGCACCTG CAGGAGCTGG TCGGCCAGGA GACACTGCCC 
AGGGACAGCC CCGACCTCCT GCTGCTGCTC CGGCTGCTGG CGCTGGGCCA GGGAGCCTGG 
GACATGATCG ACAGCCAGGT CTTCAAGGAG CCCAAGATGG AGGTAGAGCT CATCACCAGG 
TTCCTCCCGA TGCTCATGTC CTTCCTGGTG GATGACTACA CTTTCAATGT GGATCAGAAA 
CTTCCGGCTG AGGAGAAAGC CCCAGTCTCA TATCCAAACA CACTTCCCGA AAGCTTCACT 
AAGTTTCTGC AGGAGCAGCG CATGGCCTGC GAGGTGGGGC TGTACTACGT CCTGCACATC 
ACCAAGCAGA GGAACAAGAA CGCGCTCCTC CGCCTGCTGC CCGGGCTGGT GGAGACCTTT 
GGCGACTTGG CCTTTGGCGA CATCTTCCTC CACCTGCTCA CGGGCAACCT TGCGCTGCTG 
GCCGACGAAT TTGCCCTTGA GGACTTCTGC AGCAGCCTCT TCGATGGCTT CTTCCTCACC 
GCCTCTCCAA GGAAGGAGAA CGTGCACCGG CACGCGCTGC GGCTCCTCAT TCACCTGCAC 
CCCAGGGTGG CCCCGTCTAA GCTGGAGGCG TTGCAGAAGG CCCTGGAGCC TACAGGCCAG 
AGCGGAGAGG CAGTGAAGGA GCTTTACTCC CAGCTCGGCG AGAAGCTGGA ACAGCTGGAT 
CACCGGAAGC CCAGCCCGGC ACAGGCTGCG GAGACGCCGG CCCTGGAGCT GCCCCTCCCC 
AGCGTGCCCG CCCCTGCCCC GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056271.3
CDS	67..1953
Translation	LAELEGAGERGSGGPRGPAERASGVSAAAPGERAGDGAPSRAVAGASAMFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQRLTRMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVFKEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALEDFCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQAAETPALELPLPSVPAPAPL

Target ORF information:

RefSeq Version	NM_015456.4
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member B (NELFB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015456.4

ORF Insert Sequence:

CTGGCCGAGC TGGAGGGCGC CGGGGAGCGG GGCTCGGGCG GTCCCCGAGG CCCGGCGGAG 
CGGGCTTCTG GGGTGTCTGC GGCGGCGCCG GGGGAACGGG CTGGGGATGG GGCGCCTAGC 
CGGGCGGTGG CCGGGGCCTC GGCCATGTTC GCGGGGCTGC AGGACCTGGG CGTGGCCAAC 
GGCGAGGACC TGAAGGAGAC CCTGACCAAC TGCACGGAGC CGCTCAAGGC CATCGAGCAG 
TTCCAGACAG AGAATGGTGT GCTGCTGCCA TCTCTTCAGT CAGCCCTCCC CTTCTTGGAC 
CTGCACGGGA CGCCGCGGCT GGAGTTCCAC CAGTCGGTAT TCGATGAGCT GCGGGACAAG 
CTGCTGGAGC GAGTGTCAGC CATCGCTTCG GAGGGGAAGG CTGAGGAAAG GTACAAGAAG 
CTGGAAGACC TTCTGGAGAA GAGCTTTTCT CTGGTGAAGA TGCCGTCCCT GCAGCCCGTG 
GTGATGTGCG TCATGAAGCA CCTGCCCAAG GTTCCGGAGA AAAAACTGAA GCTGGTTATG 
GCTGACAAGG AGCTGTATCG AGCCTGCGCC GTGGAGGTGA AGCGGCAGAT CTGGCAAGAC 
AACCAGGCCC TCTTCGGGGA CGAGGTTTCC CCACTCCTGA AGCAGTACAT CCTGGAGAAG 
GAGAGCGCTC TCTTCAGTAC AGAGCTCTCT GTCCTGCACA ACTTTTTCAG TCCTTCCCCC 
AAGACCAGGC GCCAGGGCGA GGTGGTGCAG CGGCTGACGC GGATGGTGGG GAAGAACGTG 
AAGCTGTACG ACATGGTGCT GCAGTTTCTG CGCACGCTCT TCCTGCGCAC GCGGAATGTG 
CACTACTGCA CGCTGCGGGC TGAGCTGCTC ATGTCCCTGC ACGACCTGGA CGTGGGTGAA 
ATCTGCACCG TGGACCCGTG CCACAAGTTC ACCTGGTGCC TGGACGCCTG CATCCGAGAG 
CGGTTCGTGG ACAGCAAGAG GGCGCGGGAG CTGCAGGGGT TTCTCGATGG CGTCAAGAAG 
GGCCAGGAGC AGGTGCTGGG GGACCTGTCC ATGATCCTGT GTGACCCCTT CGCCATCAAC 
ACGCTGGCAC TGAGCACAGT CAGGCACCTG CAGGAGCTGG TCGGCCAGGA GACACTGCCC 
AGGGACAGCC CCGACCTCCT GCTGCTGCTC CGGCTGCTGG CGCTGGGCCA GGGAGCCTGG 
GACATGATCG ACAGCCAGGT CTTCAAGGAG CCCAAGATGG AGGTAGAGCT CATCACCAGG 
TTCCTCCCGA TGCTCATGTC CTTCCTGGTG GATGACTACA CTTTCAATGT GGATCAGAAA 
CTTCCGGCTG AGGAGAAAGC CCCAGTCTCA TATCCAAACA CACTTCCCGA AAGCTTCACT 
AAGTTTCTGC AGGAGCAGCG CATGGCCTGC GAGGTGGGGC TGTACTACGT CCTGCACATC 
ACCAAGCAGA GGAACAAGAA CGCGCTCCTC CGCCTGCTGC CCGGGCTGGT GGAGACCTTT 
GGCGACTTGG CCTTTGGCGA CATCTTCCTC CACCTGCTCA CGGGCAACCT TGCGCTGCTG 
GCCGACGAAT TTGCCCTTGA GGACTTCTGC AGCAGCCTCT TCGATGGCTT CTTCCTCACC 
GCCTCTCCAA GGAAGGAGAA CGTGCACCGG CACGCGCTGC GGCTCCTCAT TCACCTGCAC 
CCCAGGGTGG CCCCGTCTAA GCTGGAGGCG TTGCAGAAGG CCCTGGAGCC TACAGGCCAG 
AGCGGAGAGG CAGTGAAGGA GCTTTACTCC CAGCTCGGCG AGAAGCTGGA ACAGCTGGAT 
CACCGGAAGC CCAGCCCGGC ACAGGCTGCG GAGACGCCGG CCCTGGAGCT GCCCCTCCCC 
AGCGTGCCCG CCCCTGCCCC GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFB ( NM_015456.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056271.3 Homo sapiens negative elongation factor complex member B (NELFB), mRNA.

Price & Availability↑

CloneID	OHu66637
Clone ID Related Accession (Same CDS sequence)	NM_015456.4 , NM_015456.5
Accession Version	NM_015456.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	negative elongation factor B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA210427.1, BC019029.1, AK022651.1 and AL050280.1. On Nov 23, 2018 this sequence version replaced NM_015456.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC019029.1, AK091056.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 26010750 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGGCCGAGC TGGAGGGCGC CGGGGAGCGG GGCTCGGGCG GTCCCCGAGG CCCGGCGGAG 
CGGGCTTCTG GGGTGTCTGC GGCGGCGCCG GGGGAACGGG CTGGGGATGG GGCGCCTAGC 
CGGGCGGTGG CCGGGGCCTC GGCCATGTTC GCGGGGCTGC AGGACCTGGG CGTGGCCAAC 
GGCGAGGACC TGAAGGAGAC CCTGACCAAC TGCACGGAGC CGCTCAAGGC CATCGAGCAG 
TTCCAGACAG AGAATGGTGT GCTGCTGCCA TCTCTTCAGT CAGCCCTCCC CTTCTTGGAC 
CTGCACGGGA CGCCGCGGCT GGAGTTCCAC CAGTCGGTAT TCGATGAGCT GCGGGACAAG 
CTGCTGGAGC GAGTGTCAGC CATCGCTTCG GAGGGGAAGG CTGAGGAAAG GTACAAGAAG 
CTGGAAGACC TTCTGGAGAA GAGCTTTTCT CTGGTGAAGA TGCCGTCCCT GCAGCCCGTG 
GTGATGTGCG TCATGAAGCA CCTGCCCAAG GTTCCGGAGA AAAAACTGAA GCTGGTTATG 
GCTGACAAGG AGCTGTATCG AGCCTGCGCC GTGGAGGTGA AGCGGCAGAT CTGGCAAGAC 
AACCAGGCCC TCTTCGGGGA CGAGGTTTCC CCACTCCTGA AGCAGTACAT CCTGGAGAAG 
GAGAGCGCTC TCTTCAGTAC AGAGCTCTCT GTCCTGCACA ACTTTTTCAG TCCTTCCCCC 
AAGACCAGGC GCCAGGGCGA GGTGGTGCAG CGGCTGACGC GGATGGTGGG GAAGAACGTG 
AAGCTGTACG ACATGGTGCT GCAGTTTCTG CGCACGCTCT TCCTGCGCAC GCGGAATGTG 
CACTACTGCA CGCTGCGGGC TGAGCTGCTC ATGTCCCTGC ACGACCTGGA CGTGGGTGAA 
ATCTGCACCG TGGACCCGTG CCACAAGTTC ACCTGGTGCC TGGACGCCTG CATCCGAGAG 
CGGTTCGTGG ACAGCAAGAG GGCGCGGGAG CTGCAGGGGT TTCTCGATGG CGTCAAGAAG 
GGCCAGGAGC AGGTGCTGGG GGACCTGTCC ATGATCCTGT GTGACCCCTT CGCCATCAAC 
ACGCTGGCAC TGAGCACAGT CAGGCACCTG CAGGAGCTGG TCGGCCAGGA GACACTGCCC 
AGGGACAGCC CCGACCTCCT GCTGCTGCTC CGGCTGCTGG CGCTGGGCCA GGGAGCCTGG 
GACATGATCG ACAGCCAGGT CTTCAAGGAG CCCAAGATGG AGGTAGAGCT CATCACCAGG 
TTCCTCCCGA TGCTCATGTC CTTCCTGGTG GATGACTACA CTTTCAATGT GGATCAGAAA 
CTTCCGGCTG AGGAGAAAGC CCCAGTCTCA TATCCAAACA CACTTCCCGA AAGCTTCACT 
AAGTTTCTGC AGGAGCAGCG CATGGCCTGC GAGGTGGGGC TGTACTACGT CCTGCACATC 
ACCAAGCAGA GGAACAAGAA CGCGCTCCTC CGCCTGCTGC CCGGGCTGGT GGAGACCTTT 
GGCGACTTGG CCTTTGGCGA CATCTTCCTC CACCTGCTCA CGGGCAACCT TGCGCTGCTG 
GCCGACGAAT TTGCCCTTGA GGACTTCTGC AGCAGCCTCT TCGATGGCTT CTTCCTCACC 
GCCTCTCCAA GGAAGGAGAA CGTGCACCGG CACGCGCTGC GGCTCCTCAT TCACCTGCAC 
CCCAGGGTGG CCCCGTCTAA GCTGGAGGCG TTGCAGAAGG CCCTGGAGCC TACAGGCCAG 
AGCGGAGAGG CAGTGAAGGA GCTTTACTCC CAGCTCGGCG AGAAGCTGGA ACAGCTGGAT 
CACCGGAAGC CCAGCCCGGC ACAGGCTGCG GAGACGCCGG CCCTGGAGCT GCCCCTCCCC 
AGCGTGCCCG CCCCTGCCCC GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056271.3
CDS	40..1926
Translation	LAELEGAGERGSGGPRGPAERASGVSAAAPGERAGDGAPSRAVAGASAMFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQRLTRMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVFKEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALEDFCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQAAETPALELPLPSVPAPAPL

Target ORF information:

RefSeq Version	NM_015456.5
Organism	Homo sapiens(human)
Definition	Homo sapiens negative elongation factor complex member B (NELFB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015456.5

ORF Insert Sequence:

CTGGCCGAGC TGGAGGGCGC CGGGGAGCGG GGCTCGGGCG GTCCCCGAGG CCCGGCGGAG 
CGGGCTTCTG GGGTGTCTGC GGCGGCGCCG GGGGAACGGG CTGGGGATGG GGCGCCTAGC 
CGGGCGGTGG CCGGGGCCTC GGCCATGTTC GCGGGGCTGC AGGACCTGGG CGTGGCCAAC 
GGCGAGGACC TGAAGGAGAC CCTGACCAAC TGCACGGAGC CGCTCAAGGC CATCGAGCAG 
TTCCAGACAG AGAATGGTGT GCTGCTGCCA TCTCTTCAGT CAGCCCTCCC CTTCTTGGAC 
CTGCACGGGA CGCCGCGGCT GGAGTTCCAC CAGTCGGTAT TCGATGAGCT GCGGGACAAG 
CTGCTGGAGC GAGTGTCAGC CATCGCTTCG GAGGGGAAGG CTGAGGAAAG GTACAAGAAG 
CTGGAAGACC TTCTGGAGAA GAGCTTTTCT CTGGTGAAGA TGCCGTCCCT GCAGCCCGTG 
GTGATGTGCG TCATGAAGCA CCTGCCCAAG GTTCCGGAGA AAAAACTGAA GCTGGTTATG 
GCTGACAAGG AGCTGTATCG AGCCTGCGCC GTGGAGGTGA AGCGGCAGAT CTGGCAAGAC 
AACCAGGCCC TCTTCGGGGA CGAGGTTTCC CCACTCCTGA AGCAGTACAT CCTGGAGAAG 
GAGAGCGCTC TCTTCAGTAC AGAGCTCTCT GTCCTGCACA ACTTTTTCAG TCCTTCCCCC 
AAGACCAGGC GCCAGGGCGA GGTGGTGCAG CGGCTGACGC GGATGGTGGG GAAGAACGTG 
AAGCTGTACG ACATGGTGCT GCAGTTTCTG CGCACGCTCT TCCTGCGCAC GCGGAATGTG 
CACTACTGCA CGCTGCGGGC TGAGCTGCTC ATGTCCCTGC ACGACCTGGA CGTGGGTGAA 
ATCTGCACCG TGGACCCGTG CCACAAGTTC ACCTGGTGCC TGGACGCCTG CATCCGAGAG 
CGGTTCGTGG ACAGCAAGAG GGCGCGGGAG CTGCAGGGGT TTCTCGATGG CGTCAAGAAG 
GGCCAGGAGC AGGTGCTGGG GGACCTGTCC ATGATCCTGT GTGACCCCTT CGCCATCAAC 
ACGCTGGCAC TGAGCACAGT CAGGCACCTG CAGGAGCTGG TCGGCCAGGA GACACTGCCC 
AGGGACAGCC CCGACCTCCT GCTGCTGCTC CGGCTGCTGG CGCTGGGCCA GGGAGCCTGG 
GACATGATCG ACAGCCAGGT CTTCAAGGAG CCCAAGATGG AGGTAGAGCT CATCACCAGG 
TTCCTCCCGA TGCTCATGTC CTTCCTGGTG GATGACTACA CTTTCAATGT GGATCAGAAA 
CTTCCGGCTG AGGAGAAAGC CCCAGTCTCA TATCCAAACA CACTTCCCGA AAGCTTCACT 
AAGTTTCTGC AGGAGCAGCG CATGGCCTGC GAGGTGGGGC TGTACTACGT CCTGCACATC 
ACCAAGCAGA GGAACAAGAA CGCGCTCCTC CGCCTGCTGC CCGGGCTGGT GGAGACCTTT 
GGCGACTTGG CCTTTGGCGA CATCTTCCTC CACCTGCTCA CGGGCAACCT TGCGCTGCTG 
GCCGACGAAT TTGCCCTTGA GGACTTCTGC AGCAGCCTCT TCGATGGCTT CTTCCTCACC 
GCCTCTCCAA GGAAGGAGAA CGTGCACCGG CACGCGCTGC GGCTCCTCAT TCACCTGCAC 
CCCAGGGTGG CCCCGTCTAA GCTGGAGGCG TTGCAGAAGG CCCTGGAGCC TACAGGCCAG 
AGCGGAGAGG CAGTGAAGGA GCTTTACTCC CAGCTCGGCG AGAAGCTGGA ACAGCTGGAT 
CACCGGAAGC CCAGCCCGGC ACAGGCTGCG GAGACGCCGG CCCTGGAGCT GCCCCTCCCC 
AGCGTGCCCG CCCCTGCCCC GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Knockdown of COBRA1 decreases the proliferation and migration of hepatocellular carcinoma cells.
Oncology reports37(3)1896-1906(2017 Mar)
El Zeneini E,Kamel S,El-Meteini M,Amleh A

Architecture and RNA binding of the human negative elongation factor.
eLife5(2016 06)
Vos SM,P?llmann D,Caizzi L,Hofmann KB,Rombaut P,Zimniak T,Herzog F,Cramer P

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

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NELFB cDNA ORF clone, Homo sapiens(human)

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ORF Insert Sequence:

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