Tbc1d32 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tbc1d32
Entrez Gene ID	544696
Full Name	TBC1 domain family, member 32
Synonyms	Bromi,C6orf170,D630037F22Rik,b2b2284Clo
General protein information	Preferred Names TBC1 domain family, member 32 Names protein broad-minded
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 B3-B4

mRNA and Protein(s)

mRNA	Protein	Name
XM_011243192.3	XP_011241494.1	protein broad-minded isoform X2
XM_011243191.3	XP_011241493.1	protein broad-minded isoform X1
XM_011243191.2	XP_011241493.1	protein broad-minded isoform X1
XM_011243192.2	XP_011241494.1	protein broad-minded isoform X2
NM_001033385.3	NP_001028557.2	protein broad-minded

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	C6H6orf170	XP_001164077.1
Canis lupus familiaris (dog)	C1H6orf170	XP_005615658.1
Danio rerio (zebrafish)	tbc1d32	NP_001093493.1
	LOC714447	XP_002808448.1
Bos taurus (cattle)	TBC1D32	XP_002690121.2
Mus musculus (house mouse)	Tbc1d32	NP_001028557.2
Homo sapiens (human)	TBC1D32	NP_689943.4
Gallus gallus (chicken)	C6ORF170	XP_004940317.1

Gene Ontology
Bibliography

Related articles in PubMed

Cell Cycle-Related Kinase (CCRK) regulates ciliogenesis and Hedgehog signaling in mice.
Snouffer A, Brown D, Lee H, Walsh J, Lupu F, Norman R, Lechtreck K, Ko HW, Eggenschwiler J
PLoS genetics13(8)e1006912(2017 Aug)

Genetic link between renal birth defects and congenital heart disease.
San Agustin JT, Klena N, Granath K, Panigrahy A, Stewart E, Devine W, Strittmatter L, Jonassen JA, Liu X, Lo CW, Pazour GJ
Nature communications711103(2016 Mar)

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y, Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J, Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury L, Ganapathiraju MK, Tobita K, Pazour GJ, Lo CW
Nature521(7553)520-4(2015 May)

Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction.
Ko HW, Norman RX, Tran J, Fuller KP, Fukuda M, Eggenschwiler JT
Developmental cell18(2)237-47(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bromi, a previously uncharacterized TBC domain-containing protein, is required for proper cellular responses to the highest levels of Shh signaling in the neural tube. (bromi, broad-minded)
Title: Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction.

The following Tbc1d32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tbc1d32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu75299	XM_011243192.3 Latest version!	Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu75298	XM_011243191.3 Latest version!	Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20978	NM_001033385.3 Latest version!	Mus musculus TBC1 domain family, member 32 (Tbc1d32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu75298	XM_011243191.2	Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu75299	XM_011243192.2	Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tbc1d32 ( XM_011243192.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241494.1 Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu75299
Clone ID Related Accession (Same CDS sequence)	XM_011243192.3 , XM_011243192.2
Accession Version	XM_011243192.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein broad-minded isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATT TCTCCAGCGA GGATGAGGTG ATGCTGCAGG CGATGCTCCG GCAGCTGTTC 
CAGAGCGTGA AGGAGAAAAT CACCGGAGCT CCCTCCTTGG AGTGCGCAGA GGAGATCCTC 
TTGCGGCTGG AGGAGACTGA CGAAAATTTT CACAACTATG AATTTGTGAA ATACCTCAGA 
CAGCACATAT GTAACATGTT GGGGTCTATG ATTGAAGAAG AAATGGAAAA ATGCACATCT 
GATCAGAATC AGGGGGAGGA TTCTGGCTAT GACACGGTTG TTCAGCATGT TACCAAAAGA 
ACGCAGGAAT CTAAAGAGTA TAAGGAAATG ATGCATTCCC TGAAGAACAT TATGATGGTG 
GTGGTCGAGG CGATGATCAA CAAGTTTGAG GAAGACGAGA CACGGAGTGA GGACAGGCAG 
AGGAAAATGC AGAGTGGCAG CTGCTGCACA GACAACTGCT CCGACAGTGA CTCCTCCTTC 
AATCAGAGTT ACAAATTCTG TCAAGGGAAA CTACGGTTGA TTTTAGACCA GTTGGATCCT 
GGCCAACCTA AAGAGGTGAG ATATGAAGCT TTACAAACAT TGTGCTCAGC TCCCCCATCC 
GACGTCCTGA GCTGTGAGAA CTGGACCACT CTCTGTGAAA AGCTGACCAC ATCTCTGTCT 
GATCCAGATC CTATGTTCAC TGACCGGATT TTAAAGTTCT ACGCACAGAC TTTTACCCTC 
TCACCGTTAC ATATGACCAA GGAGATTTAT ACAAGCTTAG CTAAATATCT GGAGGTATAT 
TTTCTTTCTA GAGAAAATCA CCTTCCTACT CTTTCAACTG GAGTAGACAT AACCAGTCCC 
AATGTGACCC GCTTACTGAA AAAGGTTCGC CTTCTGAATG AGTACCAGAA AGAGGCTCCA 
TCTTTTTGGA TTCGTCACCC AGAGAAGTAT ATGGAGGAAA TTGTGGAGAG TACTCTGTCC 
TTGCTCTCAG TCAAACACGA GCAAAGCCAC CTGGTGCCAC AGAAGATTTT GGATCCCATC 
TATTTTTTCG CATTAGTTGA TACTAAGGCT GTGTGGTTCA AAAAGTGGAT GCATGCATAT 
TACAGCAGAA CTGCAGTACT GAGACTTCTT GAAAAGAAAT ATAAATGTCT GATAACTACA 
GCAGTTCAGC AATGTGTTCA GTACTTGGAG TTATGTGAAG CTATGAAAGC TGATGAAATT 
CTGAGACATC CAAAACATTG TGGAACCAAG CAAAAGAGTT TCTACTATTC AGGACAAGAA 
TTGCAATATA TTTATTTCAT TCACTCACTG TGCCTTTTAG GAAGATTGTT GATCTATACA 
CAGGGCAGGA AATTATTCCC CATAAAGCTG AAGAATAGAA AAGATTCAGT ATCCCTGACA 
AATCTGCTGG TTTTGTTTAC TCAACTTATC TATTATTCAC CAAGTTGTCC AAAGATGACC 
TCCATCATGT GTTCAGAGAA CTATTCTCCT GCAAGTATGG TGACTGACGT TCTGCGGATG 
CTCTGCGACC AGAAAGAATG TGCAGTCGAG TGCTTATACA ACAGCACAGT GACAGAGGCG 
CTTCTCCTGC CCATTCATAA CCTGACGAAA GGAACTGCGG CTGCTCCAGA TTGCTCTGAG 
ACAGCTCTAA TTCATATAGC TGATATTTTG GCAAGAATCG CTTCTGTCGA GGAAGGACTC 
ATCTTACTTC TGTATGGAGA AAATATGAAC TCTTCTGAAG AAGAAAGTCT TACAGGTGCT 
CATATAATAG CCAAGTTTTC AAAGAAACTT CTTGAAGAAG ATATTTCTAT TTTTTCTGGA 
TCGGAAATGT TGCCTGTAGT TAAAGGAGCT TTTATTTCTG TGTGTCGTCA GATCTACGGT 
ACCTGCGAAG GCTTACAGGT GTTGCTTCCT TATGGTTTAC ATGAATCCAT AGCAAAGGCA 
TGGAAGAAGA CAAGCTTGCT ATCAGAAAGG ATCCCCACTC CAGTAGAGGG CTCTGATTCT 
GTTTCCTCAG TGAGCCAGGT GTCTCCGAAC AGTGTGGCTT GGGAAGACAA TTTGTTGGAT 
GATTTACTGA ATTTTGCAGC CACCCCCAAA GGACTGCTGC TTCTTCAGAG AACAGGCGCC 
ATCAATGAAT GTGTGACGTT TATGCTCAGC CAGTATGCAA AAAAGCCACA GGTCAACAGG 
CAGAAGAAGT TTGGCTATGA AGTCTTGGTT GTACAGGTGG CATCGACAGC AGCAGGTGCA 
GTGGCTCTAC AGAATTCAGG CTTTATTAGT GCACTTATAA CTGAGTTATG GTCCAACCTT 
GAATGTGGAA GAGATGATGT AAGGCTAACT CATCCCAGAG CTACCCCAGT GGACCCAATT 
GACCGAAGCT GTCAGAAGTC CTTCCTTGCA CTGGTGAACC TGCTGTCCTA CCCTGCTGTG 
TATGAGCTAA CAGCCAATCA GGAGCTTCCT AATAAGGCAG AGTATTCTCT TCGTGAAGTT 
CCCACATGCA TCATCGATAT AATGGATAGG CTTATTGTTT TGAACTCTGA AGCCAAGATC 
CGGTCTTTAC TGAATTATGA ACAGTCGCAC ACCTTTGGTC TAAGGTCCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241494.1
CDS	825..3398
Translation	MAHFSSEDEVMLQAMLRQLFQSVKEKITGAPSLECAEEILLRLEETDENFHNYEFVKYLRQHICNMLGSMIEEEMEKCTSDQNQGEDSGYDTVVQHVTKRTQESKEYKEMMHSLKNIMMVVVEAMINKFEEDETRSEDRQRKMQSGSCCTDNCSDSDSSFNQSYKFCQGKLRLILDQLDPGQPKEVRYEALQTLCSAPPSDVLSCENWTTLCEKLTTSLSDPDPMFTDRILKFYAQTFTLSPLHMTKEIYTSLAKYLEVYFLSRENHLPTLSTGVDITSPNVTRLLKKVRLLNEYQKEAPSFWIRHPEKYMEEIVESTLSLLSVKHEQSHLVPQKILDPIYFFALVDTKAVWFKKWMHAYYSRTAVLRLLEKKYKCLITTAVQQCVQYLELCEAMKADEILRHPKHCGTKQKSFYYSGQELQYIYFIHSLCLLGRLLIYTQGRKLFPIKLKNRKDSVSLTNLLVLFTQLIYYSPSCPKMTSIMCSENYSPASMVTDVLRMLCDQKECAVECLYNSTVTEALLLPIHNLTKGTAAAPDCSETALIHIADILARIASVEEGLILLLYGENMNSSEEESLTGAHIIAKFSKKLLEEDISIFSGSEMLPVVKGAFISVCRQIYGTCEGLQVLLPYGLHESIAKAWKKTSLLSERIPTPVEGSDSVSSVSQVSPNSVAWEDNLLDDLLNFAATPKGLLLLQRTGAINECVTFMLSQYAKKPQVNRQKKFGYEVLVVQVASTAAGAVALQNSGFISALITELWSNLECGRDDVRLTHPRATPVDPIDRSCQKSFLALVNLLSYPAVYELTANQELPNKAEYSLREVPTCIIDIMDRLIVLNSEAKIRSLLNYEQSHTFGLRSL

Target ORF information:

RefSeq Version	XM_011243192.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243192.2

ORF Insert Sequence:

ATGGCCCATT TCTCCAGCGA GGATGAGGTG ATGCTGCAGG CGATGCTCCG GCAGCTGTTC 
CAGAGCGTGA AGGAGAAAAT CACCGGAGCT CCCTCCTTGG AGTGCGCAGA GGAGATCCTC 
TTGCGGCTGG AGGAGACTGA CGAAAATTTT CACAACTATG AATTTGTGAA ATACCTCAGA 
CAGCACATAT GTAACATGTT GGGGTCTATG ATTGAAGAAG AAATGGAAAA ATGCACATCT 
GATCAGAATC AGGGGGAGGA TTCTGGCTAT GACACGGTTG TTCAGCATGT TACCAAAAGA 
ACGCAGGAAT CTAAAGAGTA TAAGGAAATG ATGCATTCCC TGAAGAACAT TATGATGGTG 
GTGGTCGAGG CGATGATCAA CAAGTTTGAG GAAGACGAGA CACGGAGTGA GGACAGGCAG 
AGGAAAATGC AGAGTGGCAG CTGCTGCACA GACAACTGCT CCGACAGTGA CTCCTCCTTC 
AATCAGAGTT ACAAATTCTG TCAAGGGAAA CTACGGTTGA TTTTAGACCA GTTGGATCCT 
GGCCAACCTA AAGAGGTGAG ATATGAAGCT TTACAAACAT TGTGCTCAGC TCCCCCATCC 
GACGTCCTGA GCTGTGAGAA CTGGACCACT CTCTGTGAAA AGCTGACCAC ATCTCTGTCT 
GATCCAGATC CTATGTTCAC TGACCGGATT TTAAAGTTCT ACGCACAGAC TTTTACCCTC 
TCACCGTTAC ATATGACCAA GGAGATTTAT ACAAGCTTAG CTAAATATCT GGAGGTATAT 
TTTCTTTCTA GAGAAAATCA CCTTCCTACT CTTTCAACTG GAGTAGACAT AACCAGTCCC 
AATGTGACCC GCTTACTGAA AAAGGTTCGC CTTCTGAATG AGTACCAGAA AGAGGCTCCA 
TCTTTTTGGA TTCGTCACCC AGAGAAGTAT ATGGAGGAAA TTGTGGAGAG TACTCTGTCC 
TTGCTCTCAG TCAAACACGA GCAAAGCCAC CTGGTGCCAC AGAAGATTTT GGATCCCATC 
TATTTTTTCG CATTAGTTGA TACTAAGGCT GTGTGGTTCA AAAAGTGGAT GCATGCATAT 
TACAGCAGAA CTGCAGTACT GAGACTTCTT GAAAAGAAAT ATAAATGTCT GATAACTACA 
GCAGTTCAGC AATGTGTTCA GTACTTGGAG TTATGTGAAG CTATGAAAGC TGATGAAATT 
CTGAGACATC CAAAACATTG TGGAACCAAG CAAAAGAGTT TCTACTATTC AGGACAAGAA 
TTGCAATATA TTTATTTCAT TCACTCACTG TGCCTTTTAG GAAGATTGTT GATCTATACA 
CAGGGCAGGA AATTATTCCC CATAAAGCTG AAGAATAGAA AAGATTCAGT ATCCCTGACA 
AATCTGCTGG TTTTGTTTAC TCAACTTATC TATTATTCAC CAAGTTGTCC AAAGATGACC 
TCCATCATGT GTTCAGAGAA CTATTCTCCT GCAAGTATGG TGACTGACGT TCTGCGGATG 
CTCTGCGACC AGAAAGAATG TGCAGTCGAG TGCTTATACA ACAGCACAGT GACAGAGGCG 
CTTCTCCTGC CCATTCATAA CCTGACGAAA GGAACTGCGG CTGCTCCAGA TTGCTCTGAG 
ACAGCTCTAA TTCATATAGC TGATATTTTG GCAAGAATCG CTTCTGTCGA GGAAGGACTC 
ATCTTACTTC TGTATGGAGA AAATATGAAC TCTTCTGAAG AAGAAAGTCT TACAGGTGCT 
CATATAATAG CCAAGTTTTC AAAGAAACTT CTTGAAGAAG ATATTTCTAT TTTTTCTGGA 
TCGGAAATGT TGCCTGTAGT TAAAGGAGCT TTTATTTCTG TGTGTCGTCA GATCTACGGT 
ACCTGCGAAG GCTTACAGGT GTTGCTTCCT TATGGTTTAC ATGAATCCAT AGCAAAGGCA 
TGGAAGAAGA CAAGCTTGCT ATCAGAAAGG ATCCCCACTC CAGTAGAGGG CTCTGATTCT 
GTTTCCTCAG TGAGCCAGGT GTCTCCGAAC AGTGTGGCTT GGGAAGACAA TTTGTTGGAT 
GATTTACTGA ATTTTGCAGC CACCCCCAAA GGACTGCTGC TTCTTCAGAG AACAGGCGCC 
ATCAATGAAT GTGTGACGTT TATGCTCAGC CAGTATGCAA AAAAGCCACA GGTCAACAGG 
CAGAAGAAGT TTGGCTATGA AGTCTTGGTT GTACAGGTGG CATCGACAGC AGCAGGTGCA 
GTGGCTCTAC AGAATTCAGG CTTTATTAGT GCACTTATAA CTGAGTTATG GTCCAACCTT 
GAATGTGGAA GAGATGATGT AAGGCTAACT CATCCCAGAG CTACCCCAGT GGACCCAATT 
GACCGAAGCT GTCAGAAGTC CTTCCTTGCA CTGGTGAACC TGCTGTCCTA CCCTGCTGTG 
TATGAGCTAA CAGCCAATCA GGAGCTTCCT AATAAGGCAG AGTATTCTCT TCGTGAAGTT 
CCCACATGCA TCATCGATAT AATGGATAGG CTTATTGTTT TGAACTCTGA AGCCAAGATC 
CGGTCTTTAC TGAATTATGA ACAGTCGCAC ACCTTTGGTC TAAGGTCCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbc1d32 ( XM_011243192.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241494.1 Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu75299
Clone ID Related Accession (Same CDS sequence)	XM_011243192.3 , XM_011243192.2
Accession Version	XM_011243192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein broad-minded isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011243192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATT TCTCCAGCGA GGATGAGGTG ATGCTGCAGG CGATGCTCCG GCAGCTGTTC 
CAGAGCGTGA AGGAGAAAAT CACCGGAGCT CCCTCCTTGG AGTGCGCAGA GGAGATCCTC 
TTGCGGCTGG AGGAGACTGA CGAAAATTTT CACAACTATG AATTTGTGAA ATACCTCAGA 
CAGCACATAT GTAACATGTT GGGGTCTATG ATTGAAGAAG AAATGGAAAA ATGCACATCT 
GATCAGAATC AGGGGGAGGA TTCTGGCTAT GACACGGTTG TTCAGCATGT TACCAAAAGA 
ACGCAGGAAT CTAAAGAGTA TAAGGAAATG ATGCATTCCC TGAAGAACAT TATGATGGTG 
GTGGTCGAGG CGATGATCAA CAAGTTTGAG GAAGACGAGA CACGGAGTGA GGACAGGCAG 
AGGAAAATGC AGAGTGGCAG CTGCTGCACA GACAACTGCT CCGACAGTGA CTCCTCCTTC 
AATCAGAGTT ACAAATTCTG TCAAGGGAAA CTACGGTTGA TTTTAGACCA GTTGGATCCT 
GGCCAACCTA AAGAGGTGAG ATATGAAGCT TTACAAACAT TGTGCTCAGC TCCCCCATCC 
GACGTCCTGA GCTGTGAGAA CTGGACCACT CTCTGTGAAA AGCTGACCAC ATCTCTGTCT 
GATCCAGATC CTATGTTCAC TGACCGGATT TTAAAGTTCT ACGCACAGAC TTTTACCCTC 
TCACCGTTAC ATATGACCAA GGAGATTTAT ACAAGCTTAG CTAAATATCT GGAGGTATAT 
TTTCTTTCTA GAGAAAATCA CCTTCCTACT CTTTCAACTG GAGTAGACAT AACCAGTCCC 
AATGTGACCC GCTTACTGAA AAAGGTTCGC CTTCTGAATG AGTACCAGAA AGAGGCTCCA 
TCTTTTTGGA TTCGTCACCC AGAGAAGTAT ATGGAGGAAA TTGTGGAGAG TACTCTGTCC 
TTGCTCTCAG TCAAACACGA GCAAAGCCAC CTGGTGCCAC AGAAGATTTT GGATCCCATC 
TATTTTTTCG CATTAGTTGA TACTAAGGCT GTGTGGTTCA AAAAGTGGAT GCATGCATAT 
TACAGCAGAA CTGCAGTACT GAGACTTCTT GAAAAGAAAT ATAAATGTCT GATAACTACA 
GCAGTTCAGC AATGTGTTCA GTACTTGGAG TTATGTGAAG CTATGAAAGC TGATGAAATT 
CTGAGACATC CAAAACATTG TGGAACCAAG CAAAAGAGTT TCTACTATTC AGGACAAGAA 
TTGCAATATA TTTATTTCAT TCACTCACTG TGCCTTTTAG GAAGATTGTT GATCTATACA 
CAGGGCAGGA AATTATTCCC CATAAAGCTG AAGAATAGAA AAGATTCAGT ATCCCTGACA 
AATCTGCTGG TTTTGTTTAC TCAACTTATC TATTATTCAC CAAGTTGTCC AAAGATGACC 
TCCATCATGT GTTCAGAGAA CTATTCTCCT GCAAGTATGG TGACTGACGT TCTGCGGATG 
CTCTGCGACC AGAAAGAATG TGCAGTCGAG TGCTTATACA ACAGCACAGT GACAGAGGCG 
CTTCTCCTGC CCATTCATAA CCTGACGAAA GGAACTGCGG CTGCTCCAGA TTGCTCTGAG 
ACAGCTCTAA TTCATATAGC TGATATTTTG GCAAGAATCG CTTCTGTCGA GGAAGGACTC 
ATCTTACTTC TGTATGGAGA AAATATGAAC TCTTCTGAAG AAGAAAGTCT TACAGGTGCT 
CATATAATAG CCAAGTTTTC AAAGAAACTT CTTGAAGAAG ATATTTCTAT TTTTTCTGGA 
TCGGAAATGT TGCCTGTAGT TAAAGGAGCT TTTATTTCTG TGTGTCGTCA GATCTACGGT 
ACCTGCGAAG GCTTACAGGT GTTGCTTCCT TATGGTTTAC ATGAATCCAT AGCAAAGGCA 
TGGAAGAAGA CAAGCTTGCT ATCAGAAAGG ATCCCCACTC CAGTAGAGGG CTCTGATTCT 
GTTTCCTCAG TGAGCCAGGT GTCTCCGAAC AGTGTGGCTT GGGAAGACAA TTTGTTGGAT 
GATTTACTGA ATTTTGCAGC CACCCCCAAA GGACTGCTGC TTCTTCAGAG AACAGGCGCC 
ATCAATGAAT GTGTGACGTT TATGCTCAGC CAGTATGCAA AAAAGCCACA GGTCAACAGG 
CAGAAGAAGT TTGGCTATGA AGTCTTGGTT GTACAGGTGG CATCGACAGC AGCAGGTGCA 
GTGGCTCTAC AGAATTCAGG CTTTATTAGT GCACTTATAA CTGAGTTATG GTCCAACCTT 
GAATGTGGAA GAGATGATGT AAGGCTAACT CATCCCAGAG CTACCCCAGT GGACCCAATT 
GACCGAAGCT GTCAGAAGTC CTTCCTTGCA CTGGTGAACC TGCTGTCCTA CCCTGCTGTG 
TATGAGCTAA CAGCCAATCA GGAGCTTCCT AATAAGGCAG AGTATTCTCT TCGTGAAGTT 
CCCACATGCA TCATCGATAT AATGGATAGG CTTATTGTTT TGAACTCTGA AGCCAAGATC 
CGGTCTTTAC TGAATTATGA ACAGTCGCAC ACCTTTGGTC TAAGGTCCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241494.1
CDS	336..2909
Translation	MAHFSSEDEVMLQAMLRQLFQSVKEKITGAPSLECAEEILLRLEETDENFHNYEFVKYLRQHICNMLGSMIEEEMEKCTSDQNQGEDSGYDTVVQHVTKRTQESKEYKEMMHSLKNIMMVVVEAMINKFEEDETRSEDRQRKMQSGSCCTDNCSDSDSSFNQSYKFCQGKLRLILDQLDPGQPKEVRYEALQTLCSAPPSDVLSCENWTTLCEKLTTSLSDPDPMFTDRILKFYAQTFTLSPLHMTKEIYTSLAKYLEVYFLSRENHLPTLSTGVDITSPNVTRLLKKVRLLNEYQKEAPSFWIRHPEKYMEEIVESTLSLLSVKHEQSHLVPQKILDPIYFFALVDTKAVWFKKWMHAYYSRTAVLRLLEKKYKCLITTAVQQCVQYLELCEAMKADEILRHPKHCGTKQKSFYYSGQELQYIYFIHSLCLLGRLLIYTQGRKLFPIKLKNRKDSVSLTNLLVLFTQLIYYSPSCPKMTSIMCSENYSPASMVTDVLRMLCDQKECAVECLYNSTVTEALLLPIHNLTKGTAAAPDCSETALIHIADILARIASVEEGLILLLYGENMNSSEEESLTGAHIIAKFSKKLLEEDISIFSGSEMLPVVKGAFISVCRQIYGTCEGLQVLLPYGLHESIAKAWKKTSLLSERIPTPVEGSDSVSSVSQVSPNSVAWEDNLLDDLLNFAATPKGLLLLQRTGAINECVTFMLSQYAKKPQVNRQKKFGYEVLVVQVASTAAGAVALQNSGFISALITELWSNLECGRDDVRLTHPRATPVDPIDRSCQKSFLALVNLLSYPAVYELTANQELPNKAEYSLREVPTCIIDIMDRLIVLNSEAKIRSLLNYEQSHTFGLRSL

Target ORF information:

RefSeq Version	XM_011243192.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243192.3

ORF Insert Sequence:

ATGGCCCATT TCTCCAGCGA GGATGAGGTG ATGCTGCAGG CGATGCTCCG GCAGCTGTTC 
CAGAGCGTGA AGGAGAAAAT CACCGGAGCT CCCTCCTTGG AGTGCGCAGA GGAGATCCTC 
TTGCGGCTGG AGGAGACTGA CGAAAATTTT CACAACTATG AATTTGTGAA ATACCTCAGA 
CAGCACATAT GTAACATGTT GGGGTCTATG ATTGAAGAAG AAATGGAAAA ATGCACATCT 
GATCAGAATC AGGGGGAGGA TTCTGGCTAT GACACGGTTG TTCAGCATGT TACCAAAAGA 
ACGCAGGAAT CTAAAGAGTA TAAGGAAATG ATGCATTCCC TGAAGAACAT TATGATGGTG 
GTGGTCGAGG CGATGATCAA CAAGTTTGAG GAAGACGAGA CACGGAGTGA GGACAGGCAG 
AGGAAAATGC AGAGTGGCAG CTGCTGCACA GACAACTGCT CCGACAGTGA CTCCTCCTTC 
AATCAGAGTT ACAAATTCTG TCAAGGGAAA CTACGGTTGA TTTTAGACCA GTTGGATCCT 
GGCCAACCTA AAGAGGTGAG ATATGAAGCT TTACAAACAT TGTGCTCAGC TCCCCCATCC 
GACGTCCTGA GCTGTGAGAA CTGGACCACT CTCTGTGAAA AGCTGACCAC ATCTCTGTCT 
GATCCAGATC CTATGTTCAC TGACCGGATT TTAAAGTTCT ACGCACAGAC TTTTACCCTC 
TCACCGTTAC ATATGACCAA GGAGATTTAT ACAAGCTTAG CTAAATATCT GGAGGTATAT 
TTTCTTTCTA GAGAAAATCA CCTTCCTACT CTTTCAACTG GAGTAGACAT AACCAGTCCC 
AATGTGACCC GCTTACTGAA AAAGGTTCGC CTTCTGAATG AGTACCAGAA AGAGGCTCCA 
TCTTTTTGGA TTCGTCACCC AGAGAAGTAT ATGGAGGAAA TTGTGGAGAG TACTCTGTCC 
TTGCTCTCAG TCAAACACGA GCAAAGCCAC CTGGTGCCAC AGAAGATTTT GGATCCCATC 
TATTTTTTCG CATTAGTTGA TACTAAGGCT GTGTGGTTCA AAAAGTGGAT GCATGCATAT 
TACAGCAGAA CTGCAGTACT GAGACTTCTT GAAAAGAAAT ATAAATGTCT GATAACTACA 
GCAGTTCAGC AATGTGTTCA GTACTTGGAG TTATGTGAAG CTATGAAAGC TGATGAAATT 
CTGAGACATC CAAAACATTG TGGAACCAAG CAAAAGAGTT TCTACTATTC AGGACAAGAA 
TTGCAATATA TTTATTTCAT TCACTCACTG TGCCTTTTAG GAAGATTGTT GATCTATACA 
CAGGGCAGGA AATTATTCCC CATAAAGCTG AAGAATAGAA AAGATTCAGT ATCCCTGACA 
AATCTGCTGG TTTTGTTTAC TCAACTTATC TATTATTCAC CAAGTTGTCC AAAGATGACC 
TCCATCATGT GTTCAGAGAA CTATTCTCCT GCAAGTATGG TGACTGACGT TCTGCGGATG 
CTCTGCGACC AGAAAGAATG TGCAGTCGAG TGCTTATACA ACAGCACAGT GACAGAGGCG 
CTTCTCCTGC CCATTCATAA CCTGACGAAA GGAACTGCGG CTGCTCCAGA TTGCTCTGAG 
ACAGCTCTAA TTCATATAGC TGATATTTTG GCAAGAATCG CTTCTGTCGA GGAAGGACTC 
ATCTTACTTC TGTATGGAGA AAATATGAAC TCTTCTGAAG AAGAAAGTCT TACAGGTGCT 
CATATAATAG CCAAGTTTTC AAAGAAACTT CTTGAAGAAG ATATTTCTAT TTTTTCTGGA 
TCGGAAATGT TGCCTGTAGT TAAAGGAGCT TTTATTTCTG TGTGTCGTCA GATCTACGGT 
ACCTGCGAAG GCTTACAGGT GTTGCTTCCT TATGGTTTAC ATGAATCCAT AGCAAAGGCA 
TGGAAGAAGA CAAGCTTGCT ATCAGAAAGG ATCCCCACTC CAGTAGAGGG CTCTGATTCT 
GTTTCCTCAG TGAGCCAGGT GTCTCCGAAC AGTGTGGCTT GGGAAGACAA TTTGTTGGAT 
GATTTACTGA ATTTTGCAGC CACCCCCAAA GGACTGCTGC TTCTTCAGAG AACAGGCGCC 
ATCAATGAAT GTGTGACGTT TATGCTCAGC CAGTATGCAA AAAAGCCACA GGTCAACAGG 
CAGAAGAAGT TTGGCTATGA AGTCTTGGTT GTACAGGTGG CATCGACAGC AGCAGGTGCA 
GTGGCTCTAC AGAATTCAGG CTTTATTAGT GCACTTATAA CTGAGTTATG GTCCAACCTT 
GAATGTGGAA GAGATGATGT AAGGCTAACT CATCCCAGAG CTACCCCAGT GGACCCAATT 
GACCGAAGCT GTCAGAAGTC CTTCCTTGCA CTGGTGAACC TGCTGTCCTA CCCTGCTGTG 
TATGAGCTAA CAGCCAATCA GGAGCTTCCT AATAAGGCAG AGTATTCTCT TCGTGAAGTT 
CCCACATGCA TCATCGATAT AATGGATAGG CTTATTGTTT TGAACTCTGA AGCCAAGATC 
CGGTCTTTAC TGAATTATGA ACAGTCGCAC ACCTTTGGTC TAAGGTCCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbc1d32 ( XM_011243191.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241493.1 Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X1, mRNA.

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CloneID	OMu75298
Clone ID Related Accession (Same CDS sequence)	XM_011243191.3 , XM_011243191.2
Accession Version	XM_011243191.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein broad-minded isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011243191.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATT TCTCCAGCGA GGATGAGGTG ATGCTGCAGG CGATGCTCCG GCAGCTGTTC 
CAGAGCGTGA AGGAGAAAAT CACCGGAGCT CCCTCCTTGG AGTGCGCAGA GGAGATCCTC 
TTGCGGCTGG AGGAGACTGA CGAAAATTTT CACAACTATG AATTTGTGAA ATACCTCAGA 
CAGCACATAT GTAACATGTT GGGGTCTATG ATTGAAGAAG AAATGGAAAA ATGCACATCT 
GATCAGAATC AGGGGGAGGA TTCTGGCTAT GACACGGTTG TTCAGCATGT TACCAAAAGA 
ACGCAGGAAT CTAAAGAGTA TAAGGAAATG ATGCATTCCC TGAAGAACAT TATGATGGTG 
GTGGTCGAGG CGATGATCAA CAAGTTTGAG GAAGACGAGA CACGGAGTGA GGACAGGCAG 
AGGAAAATGC AGAGTGGCAG CTGCTGCACA GACAACTGCT CCGACAGTGA CTCCTCCTTC 
AATCAGAGTT ACAAATTCTG TCAAGGGAAA CTACGGTTGA TTTTAGACCA GTTGGATCCT 
GGCCAACCTA AAGAGGTGAG ATATGAAGCT TTACAAACAT TGTGCTCAGC TCCCCCATCC 
GACGTCCTGA GCTGTGAGAA CTGGACCACT CTCTGTGAAA AGCTGACCAC ATCTCTGTCT 
GATCCAGATC CTATGTTCAC TGACCGGATT TTAAAGTTCT ACGCACAGAC TTTTACCCTC 
TCACCGTTAC ATATGACCAA GGAGATTTAT ACAAGCTTAG CTAAATATCT GGAGGTATAT 
TTTCTTTCTA GAGAAAATCA CCTTCCTACT CTTTCAACTG GAGTAGACAT AACCAGTCCC 
AATGTGACCC GCTTACTGAA AAAGGTTCGC CTTCTGAATG AGTACCAGAA AGAGGCTCCA 
TCTTTTTGGA TTCGTCACCC AGAGAAGTAT ATGGAGGAAA TTGTGGAGAG TACTCTGTCC 
TTGCTCTCAG TCAAACACGA GCAAAGCCAC CTGGTGCCAC AGAAGATTTT GGATCCCATC 
TATTTTTTCG CATTAGTTGA TACTAAGGCT GTGTGGTTCA AAAAGTGGAT GCATGCATAT 
TACAGCAGAA CTGCAGTACT GAGACTTCTT GAAAAGAAAT ATAAATGTCT GATAACTACA 
GCAGTTCAGC AATGTGTTCA GTACTTGGAG TTATGTGAAG CTATGAAAGC TGATGAAATT 
CTGAGACATC CAAAACATTG TGGAACCAAG CAAAAGAGTT TCTACTATTC AGGACAAGAA 
TTGCAATATA TTTATTTCAT TCACTCACTG TGCCTTTTAG GAAGATTGTT GATCTATACA 
CAGGGCAGGA AATTATTCCC CATAAAGCTG AAGAATAGAA AAGATTCAGT ATCCCTGACA 
AATCTGCTGG TTTTGTTTAC TCAACTTATC TATTATTCAC CAAGTTGTCC AAAGATGACC 
TCCATCATGT GTTCAGAGAA CTATTCTCCT GCAAGTATGG TGACTGACGT TCTGCGGATG 
CTCTGCGACC AGAAAGAATG TGCAGTCGAG TGCTTATACA ACAGCACAGT GACAGAGGCG 
CTTCTCCTGC CCATTCATAA CCTGACGAAA GGAACTGCGG CTGCTCCAGA TTGCTCTGAG 
ACAGCTCTAA TTCATATAGC TGATATTTTG GCAAGAATCG CTTCTGTCGA GGAAGGACTC 
ATCTTACTTC TGTATGGAGA AAATATGAAC TCTTCTGAAG AAGAAAGTCT TACAGGTGCT 
CATATAATAG CCAAGTTTTC AAAGAAACTT CTTGAAGAAG ATATTTCTAT TTTTTCTGGA 
TCGGAAATGT TGCCTGTAGT TAAAGGAGCT TTTATTTCTG TGTGTCGTCA GATCTACGGT 
ACCTGCGAAG GCTTACAGGT GTTGCTTCCT TATGGTTTAC ATGAATCCAT AGCAAAGGCA 
TGGAAGAAGA CAAGCTTGCT ATCAGAAAGG ATCCCCACTC CAGTAGAGGG CTCTGATTCT 
GTTTCCTCAG TGAGCCAGGT GTCTCCGAAC AGTGTGGCTT GGGAAGACAA TTTGTTGGAT 
GATTTACTGA ATTTTGCAGC CACCCCCAAA GGACTGCTGC TTCTTCAGAG AACAGGCGCC 
ATCAATGAAT GTGTGACGTT TATGCTCAGC CAGTATGCAA AAAAGCCACA GGTCAACAGG 
CAGAAGAAGT TTGGCTATGA AGTCTTGGTT GTACAGGTGG CATCGACAGC AGCAGGTGCA 
GTGGCTCTAC AGAATTCAGG CTTTATTAGT GCACTTATAA CTGAGTTATG GTCCAACCTT 
GAATGTGGAA GAGATGATGT AAGGCTAACT CATCCCAGAG CTACCCCAGT GGACCCAATT 
GACCGAAGCT GTCAGAAGTC CTTCCTTGCA CTGGTGAACC TGCTGTCCTA CCCTGCTGTG 
TATGAGCTAA CAGCCAATCA GGAGCTTCCT AATAAGGCAG AGTATTCTCT TCGTGAAGTT 
CCCACATGCA TCATCGATAT AATGGATAGG CTTATTGTTT TGAACTCTGA AGCCAAGATC 
CGGTCTTTAC TGAATTATGA ACAGTCGCAC ACCTTTGGTC TAAGGTTACT AAGTGTGGTG 
TGCTGTGATC TGGATGCGCT TCTCTTACTG GAGGCTCAGT ATCAAGTATC TAACATGTTA 
TTACATGCTC AAGAAGAAAA TACCTTTGAG ATCTCTGAGA ACCACAGGAA CTTTATAATT 
GATGGATTAT CAGTAGAGAG AAATCATGTT CTCGTAAGGA TTAATCTTAT TGGTGGTCCT 
TCAGAGCGGA TATTGCCTCC AAGGATGCTT GAGAAGGGGG ATGACCCATA TCCTTGGCCA 
ATGTTTTCTT CATATCCATT GCCACACTGC TATCAGTCAG AAGGTCCACG AAGTGCTGAT 
CTAAAACAAG ACAATGATAT TGGAAACCTG TTATCATGCT TCAAAATGTC CGATAAACAA 
ACAGAGTGGA TAGAGAACTG CCGAAGACAG TTTTGTAAAA CAATGAAGTC CAAGCCTGAT 
GCAGTCCATG GAAGTGCCTT AGGAGAACTA CTGGAAAAAT TTGTGCTGCT TCTCACCGAA 
AACCCATCTG AATGTTACTT CCCTTCAGTG GAATACACAG GTGTGTTGCC TGAGTGTACC 
ACATGTCTAT GTACCACATA TGTTCCTGGT ACTCACAGAG GCCAGAAGAA GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241493.1
CDS	337..3513
Translation	MAHFSSEDEVMLQAMLRQLFQSVKEKITGAPSLECAEEILLRLEETDENFHNYEFVKYLRQHICNMLGSMIEEEMEKCTSDQNQGEDSGYDTVVQHVTKRTQESKEYKEMMHSLKNIMMVVVEAMINKFEEDETRSEDRQRKMQSGSCCTDNCSDSDSSFNQSYKFCQGKLRLILDQLDPGQPKEVRYEALQTLCSAPPSDVLSCENWTTLCEKLTTSLSDPDPMFTDRILKFYAQTFTLSPLHMTKEIYTSLAKYLEVYFLSRENHLPTLSTGVDITSPNVTRLLKKVRLLNEYQKEAPSFWIRHPEKYMEEIVESTLSLLSVKHEQSHLVPQKILDPIYFFALVDTKAVWFKKWMHAYYSRTAVLRLLEKKYKCLITTAVQQCVQYLELCEAMKADEILRHPKHCGTKQKSFYYSGQELQYIYFIHSLCLLGRLLIYTQGRKLFPIKLKNRKDSVSLTNLLVLFTQLIYYSPSCPKMTSIMCSENYSPASMVTDVLRMLCDQKECAVECLYNSTVTEALLLPIHNLTKGTAAAPDCSETALIHIADILARIASVEEGLILLLYGENMNSSEEESLTGAHIIAKFSKKLLEEDISIFSGSEMLPVVKGAFISVCRQIYGTCEGLQVLLPYGLHESIAKAWKKTSLLSERIPTPVEGSDSVSSVSQVSPNSVAWEDNLLDDLLNFAATPKGLLLLQRTGAINECVTFMLSQYAKKPQVNRQKKFGYEVLVVQVASTAAGAVALQNSGFISALITELWSNLECGRDDVRLTHPRATPVDPIDRSCQKSFLALVNLLSYPAVYELTANQELPNKAEYSLREVPTCIIDIMDRLIVLNSEAKIRSLLNYEQSHTFGLRLLSVVCCDLDALLLLEAQYQVSNMLLHAQEENTFEISENHRNFIIDGLSVERNHVLVRINLIGGPSERILPPRMLEKGDDPYPWPMFSSYPLPHCYQSEGPRSADLKQDNDIGNLLSCFKMSDKQTEWIENCRRQFCKTMKSKPDAVHGSALGELLEKFVLLLTENPSECYFPSVEYTGVLPECTTCLCTTYVPGTHRGQKKA

Target ORF information:

RefSeq Version	XM_011243191.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243191.3

ORF Insert Sequence:

ATGGCCCATT TCTCCAGCGA GGATGAGGTG ATGCTGCAGG CGATGCTCCG GCAGCTGTTC 
CAGAGCGTGA AGGAGAAAAT CACCGGAGCT CCCTCCTTGG AGTGCGCAGA GGAGATCCTC 
TTGCGGCTGG AGGAGACTGA CGAAAATTTT CACAACTATG AATTTGTGAA ATACCTCAGA 
CAGCACATAT GTAACATGTT GGGGTCTATG ATTGAAGAAG AAATGGAAAA ATGCACATCT 
GATCAGAATC AGGGGGAGGA TTCTGGCTAT GACACGGTTG TTCAGCATGT TACCAAAAGA 
ACGCAGGAAT CTAAAGAGTA TAAGGAAATG ATGCATTCCC TGAAGAACAT TATGATGGTG 
GTGGTCGAGG CGATGATCAA CAAGTTTGAG GAAGACGAGA CACGGAGTGA GGACAGGCAG 
AGGAAAATGC AGAGTGGCAG CTGCTGCACA GACAACTGCT CCGACAGTGA CTCCTCCTTC 
AATCAGAGTT ACAAATTCTG TCAAGGGAAA CTACGGTTGA TTTTAGACCA GTTGGATCCT 
GGCCAACCTA AAGAGGTGAG ATATGAAGCT TTACAAACAT TGTGCTCAGC TCCCCCATCC 
GACGTCCTGA GCTGTGAGAA CTGGACCACT CTCTGTGAAA AGCTGACCAC ATCTCTGTCT 
GATCCAGATC CTATGTTCAC TGACCGGATT TTAAAGTTCT ACGCACAGAC TTTTACCCTC 
TCACCGTTAC ATATGACCAA GGAGATTTAT ACAAGCTTAG CTAAATATCT GGAGGTATAT 
TTTCTTTCTA GAGAAAATCA CCTTCCTACT CTTTCAACTG GAGTAGACAT AACCAGTCCC 
AATGTGACCC GCTTACTGAA AAAGGTTCGC CTTCTGAATG AGTACCAGAA AGAGGCTCCA 
TCTTTTTGGA TTCGTCACCC AGAGAAGTAT ATGGAGGAAA TTGTGGAGAG TACTCTGTCC 
TTGCTCTCAG TCAAACACGA GCAAAGCCAC CTGGTGCCAC AGAAGATTTT GGATCCCATC 
TATTTTTTCG CATTAGTTGA TACTAAGGCT GTGTGGTTCA AAAAGTGGAT GCATGCATAT 
TACAGCAGAA CTGCAGTACT GAGACTTCTT GAAAAGAAAT ATAAATGTCT GATAACTACA 
GCAGTTCAGC AATGTGTTCA GTACTTGGAG TTATGTGAAG CTATGAAAGC TGATGAAATT 
CTGAGACATC CAAAACATTG TGGAACCAAG CAAAAGAGTT TCTACTATTC AGGACAAGAA 
TTGCAATATA TTTATTTCAT TCACTCACTG TGCCTTTTAG GAAGATTGTT GATCTATACA 
CAGGGCAGGA AATTATTCCC CATAAAGCTG AAGAATAGAA AAGATTCAGT ATCCCTGACA 
AATCTGCTGG TTTTGTTTAC TCAACTTATC TATTATTCAC CAAGTTGTCC AAAGATGACC 
TCCATCATGT GTTCAGAGAA CTATTCTCCT GCAAGTATGG TGACTGACGT TCTGCGGATG 
CTCTGCGACC AGAAAGAATG TGCAGTCGAG TGCTTATACA ACAGCACAGT GACAGAGGCG 
CTTCTCCTGC CCATTCATAA CCTGACGAAA GGAACTGCGG CTGCTCCAGA TTGCTCTGAG 
ACAGCTCTAA TTCATATAGC TGATATTTTG GCAAGAATCG CTTCTGTCGA GGAAGGACTC 
ATCTTACTTC TGTATGGAGA AAATATGAAC TCTTCTGAAG AAGAAAGTCT TACAGGTGCT 
CATATAATAG CCAAGTTTTC AAAGAAACTT CTTGAAGAAG ATATTTCTAT TTTTTCTGGA 
TCGGAAATGT TGCCTGTAGT TAAAGGAGCT TTTATTTCTG TGTGTCGTCA GATCTACGGT 
ACCTGCGAAG GCTTACAGGT GTTGCTTCCT TATGGTTTAC ATGAATCCAT AGCAAAGGCA 
TGGAAGAAGA CAAGCTTGCT ATCAGAAAGG ATCCCCACTC CAGTAGAGGG CTCTGATTCT 
GTTTCCTCAG TGAGCCAGGT GTCTCCGAAC AGTGTGGCTT GGGAAGACAA TTTGTTGGAT 
GATTTACTGA ATTTTGCAGC CACCCCCAAA GGACTGCTGC TTCTTCAGAG AACAGGCGCC 
ATCAATGAAT GTGTGACGTT TATGCTCAGC CAGTATGCAA AAAAGCCACA GGTCAACAGG 
CAGAAGAAGT TTGGCTATGA AGTCTTGGTT GTACAGGTGG CATCGACAGC AGCAGGTGCA 
GTGGCTCTAC AGAATTCAGG CTTTATTAGT GCACTTATAA CTGAGTTATG GTCCAACCTT 
GAATGTGGAA GAGATGATGT AAGGCTAACT CATCCCAGAG CTACCCCAGT GGACCCAATT 
GACCGAAGCT GTCAGAAGTC CTTCCTTGCA CTGGTGAACC TGCTGTCCTA CCCTGCTGTG 
TATGAGCTAA CAGCCAATCA GGAGCTTCCT AATAAGGCAG AGTATTCTCT TCGTGAAGTT 
CCCACATGCA TCATCGATAT AATGGATAGG CTTATTGTTT TGAACTCTGA AGCCAAGATC 
CGGTCTTTAC TGAATTATGA ACAGTCGCAC ACCTTTGGTC TAAGGTTACT AAGTGTGGTG 
TGCTGTGATC TGGATGCGCT TCTCTTACTG GAGGCTCAGT ATCAAGTATC TAACATGTTA 
TTACATGCTC AAGAAGAAAA TACCTTTGAG ATCTCTGAGA ACCACAGGAA CTTTATAATT 
GATGGATTAT CAGTAGAGAG AAATCATGTT CTCGTAAGGA TTAATCTTAT TGGTGGTCCT 
TCAGAGCGGA TATTGCCTCC AAGGATGCTT GAGAAGGGGG ATGACCCATA TCCTTGGCCA 
ATGTTTTCTT CATATCCATT GCCACACTGC TATCAGTCAG AAGGTCCACG AAGTGCTGAT 
CTAAAACAAG ACAATGATAT TGGAAACCTG TTATCATGCT TCAAAATGTC CGATAAACAA 
ACAGAGTGGA TAGAGAACTG CCGAAGACAG TTTTGTAAAA CAATGAAGTC CAAGCCTGAT 
GCAGTCCATG GAAGTGCCTT AGGAGAACTA CTGGAAAAAT TTGTGCTGCT TCTCACCGAA 
AACCCATCTG AATGTTACTT CCCTTCAGTG GAATACACAG GTGTGTTGCC TGAGTGTACC 
ACATGTCTAT GTACCACATA TGTTCCTGGT ACTCACAGAG GCCAGAAGAA GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbc1d32 ( NM_001033385.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028557.2 Mus musculus TBC1 domain family, member 32 (Tbc1d32), mRNA.

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CloneID	OMu20978
Clone ID Related Accession (Same CDS sequence)	NM_001033385.3
Accession Version	NM_001033385.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	protein broad-minded
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY341016.1, CJ182214.1, CR757357.1, CR756627.1, CN536606.1, CN536044.1, AK141206.1, AK035346.1, AC155829.5 and BP761946.1. On Jun 14, 2007 this sequence version replaced NM_001033385.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCATT TCTCCAGCGA GGATGAGGTG ATGCTGCAGG CGATGCTCCG GCAGCTGTTC 
CAGAGCGTGA AGGAGAAAAT CACCGGAGCT CCCTCCTTGG AGTGCGCAGA GGAGATCCTC 
TTGCGGCTGG AGGAGACTGA CGAAAATTTT CACAACTATG AATTTGTGAA ATACCTCAGA 
CAGCACATAT GTAACATGTT GGGGTCTATG ATTGAAGAAG AAATGGAAAA ATGCACATCT 
GATCAGAATC AGGGGGAGGA TTCTGGCTAT GACACGGTTG TTCAGCATGT TACCAAAAGA 
ACGCAGGAAT CTAAAGAGTA TAAGGAAATG ATGCATTCCC TGAAGAACAT TATGATGGTG 
GTGGTCGAGG CGATGATCAA CAAGTTTGAG GAAGACGAGA CACGGAGTGA GGACAGGCAG 
AGGAAAATGC AGAGTGGCAG CTGCTGCACA GACAACTGCT CCGACAGTGA CTCCTCCTTC 
AATCAGAGTT ACAAATTCTG TCAAGGGAAA CTACGGTTGA TTTTAGACCA GTTGGATCCT 
GGCCAACCTA AAGAGGTGAG ATATGAAGCT TTACAAACAT TGTGCTCAGC TCCCCCATCC 
GACGTCCTGA GCTGTGAGAA CTGGACCACT CTCTGTGAAA AGCTGACCAC ATCTCTGTCT 
GATCCAGATC CTATGTTCAC TGACCGGATT TTAAAGTTCT ACGCACAGAC TTTTACCCTC 
TCACCGTTAC ATATGACCAA GGAGATTTAT ACAAGCTTAG CTAAATATCT GGAGGTATAT 
TTTCTTTCTA GAGAAAATCA CCTTCCTACT CTTTCAACTG GAGTAGACAT AACCAGTCCC 
AATGTGACCC GCTTACTGAA AAAGGTTCGC CTTCTGAATG AGTACCAGAA AGAGGCTCCA 
TCTTTTTGGA TTCGTCACCC AGAGAAGTAT ATGGAGGAAA TTGTGGAGAG TACTCTGTCC 
TTGCTCTCAG TCAAACACGA GCAAAGCCAC CTGGTGCCAC AGAAGATTTT GGATCCCATC 
TATTTTTTCG CATTAGTTGA TACTAAGGCT GTGTGGTTCA AAAAGTGGAT GCATGCATAT 
TACAGCAGAA CTGCAGTACT GAGACTTCTT GAAAAGAAAT ATAAATGTCT GATAACTACA 
GCAGTTCAGC AATGTGTTCA GTACTTGGAG TTATGTGAAG CTATGAAAGC TGATGAAATT 
CTGAGACATC CAAAACATTG TGGAACCAAG CAAAAGAGTT TCTACTATTC AGGACAAGAA 
TTGCAATATA TTTATTTCAT TCACTCACTG TGCCTTTTAG GAAGATTGTT GATCTATACA 
CAGGGCAGGA AATTATTCCC CATAAAGCTG AAGAATAGAA AAGATTCAGT ATCCCTGACA 
AATCTGCTGG TTTTGTTTAC TCAACTTATC TATTATTCAC CAAGTTGTCC AAAGATGACC 
TCCATCATGT GTTCAGAGAA CTATTCTCCT GCAAGTATGG TGACTGACGT TCTGCGGATG 
CTCTGCGACC AGAAAGAATG TGCAGTCGAG TGCTTATACA ACAGCACAGT GACAGAGGCG 
CTTCTCCTGC CCATTCATAA CCTGACGAAA GGAACTGCGG CTGCTCCAGA TTGCTCTGAG 
ACAGCTCTAA TTCATATAGC TGATATTTTG GCAAGAATCG CTTCTGTCGA GGAAGGACTC 
ATCTTACTTC TGTATGGAGA AAATATGAAC TCTTCTGAAG AAGAAAGTCT TACAGGTGCT 
CATATAATAG CCAAGTTTTC AAAGAAACTT CTTGAAGAAG ATATTTCTAT TTTTTCTGGA 
TCGGAAATGT TGCCTGTAGT TAAAGGAGCT TTTATTTCTG TGTGTCGTCA GATCTACGGT 
ACCTGCGAAG GCTTACAGGT GTTGCTTCCT TATGGTTTAC ATGAATCCAT AGCAAAGGCA 
TGGAAGAAGA CAAGCTTGCT ATCAGAAAGG ATCCCCACTC CAGTAGAGGG CTCTGATTCT 
GTTTCCTCAG TGAGCCAGGT GTCTCCGAAC AGTGTGGCTT GGGAAGACAA TTTGTTGGAT 
GATTTACTGA ATTTTGCAGC CACCCCCAAA GGACTGCTGC TTCTTCAGAG AACAGGCGCC 
ATCAATGAAT GTGTGACGTT TATGCTCAGC CAGTATGCAA AAAAGCCACA GGTCAACAGG 
CAGAAGAAGT TTGGCTATGA AGTCTTGGTT GTACAGGTGG CATCGACAGC AGCAGGTGCA 
GTGGCTCTAC AGAATTCAGG CTTTATTAGT GCACTTATAA CTGAGTTATG GTCCAACCTT 
GAATGTGGAA GAGATGATGT AAGGCTAACT CATCCCAGAG CTACCCCAGT GGACCCAATT 
GACCGAAGCT GTCAGAAGTC CTTCCTTGCA CTGGTGAACC TGCTGTCCTA CCCTGCTGTG 
TATGAGCTAA CAGCCAATCA GGAGCTTCCT AATAAGGCAG AGTATTCTCT TCGTGAAGTT 
CCCACATGCA TCATCGATAT AATGGATAGG CTTATTGTTT TGAACTCTGA AGCCAAGATC 
CGGTCTTTAC TGAATTATGA ACAGTCGCAC ACCTTTGGTC TAAGGTTACT AAGTGTGGTG 
TGCTGTGATC TGGATGCGCT TCTCTTACTG GAGGCTCAGT ATCAAGTATC TAACATGTTA 
TTACATGCTC AAGAAGAAAA TACCTTTGAG ATCTCTGAGA ACCACAGGAA CTTTATAATT 
GATGGATTAT CAGTAGAGAG AAATCATGTT CTCGTAAGGA TTAATCTTAT TGGTGGTCCT 
TCAGAGCGGA TATTGCCTCC AAGGATGCTT GAGAAGGGGG ATGACCCATA TCCTTGGCCA 
ATGTTTTCTT CATATCCATT GCCACACTGC TATCAGTCAG AAGGTCCACG AAGTGCTGAT 
CTAAAACAAG ACAATGATAT TGGAAACCTG TTATCATGCT TCAAAATGTC CGATAAACAA 
ACAGAGTGGA TAGAGAACTG CCGAAGACAG TTTTGTAAAA CAATGAAGTC CAAGCCTGAT 
GCAGTCCATG GAAGTGCCTT AGGAGAACTA CTGGAAAAAT TTGTGCTGCT TCTCACCGAA 
AACCCATCTG AATGTTACTT CCCTTCAGTG GAATACACAG CTACTGATGC AAATGTGAAG 
AATGAAAGTC TCTCATCTGT GCAGCAGCTT GGCATGAAAA TGACTGTCAG GTATGGGAGG 
TTTCTCAATT TGTTAAAAGA TGGTGCAGAA AATGAGCTTG CTTTGGTCTT AAAGCATTGT 
GAGAAATTCC TGAAACAACA GCAGTCACCG GTCACTTCTT CTCTCCTGTG CCTGCAAGGG 
AACTACGCAG GCCATGACTG GTTTGTGTCT TCTCTGTTCA TGATAATGCT GGGAGACAAA 
GGGAAGACCT TCCACTTTCT CCAGCATTTC TCCAGGCTGC TGACCTCTGC CTTCCTTTGG 
GTGCCACGGC TGCATAATTC TAGGTACCTT CCTGTCGACA CCCTAGGGAC CGGCATCCAT 
CCCATCTACT TTTGCAGTGC TCACTACATT GAAATGCTGC TGAAGGCTGA GGTGCCGCTT 
GTGTTTTCGG CTTTCCACAT GTCTGGTTTT GCTCCATCCC AGATTTGCCT GCAGTGGATA 
ACTCAGTGTT TTTGGAATTA CTTAGATTGG ATAGAAATAT GCCACTATAT TGCTACTTGT 
GTTGTCCTTG GTCCTGATTA TCAAGTGTAT GTCTGCATAG CCGTTCTCAA ACATCTGCAG 
CGAGATATTC TCCAGCATAC CCAGACTCAA GATCTGCAAG TCTTTCTCAA AGAAGAAGCA 
CTGCATGGGT TTCGAGTGAG CAACTATTTT GAATACATGG AGAATTTGGA GCAAAACTAC 
CGACCAGTGC TGCTGCGAGA CATGCGCAGC ATCAGAGTGC AGAACACATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028557.2
CDS	58..3948
Translation	MAHFSSEDEVMLQAMLRQLFQSVKEKITGAPSLECAEEILLRLEETDENFHNYEFVKYLRQHICNMLGSMIEEEMEKCTSDQNQGEDSGYDTVVQHVTKRTQESKEYKEMMHSLKNIMMVVVEAMINKFEEDETRSEDRQRKMQSGSCCTDNCSDSDSSFNQSYKFCQGKLRLILDQLDPGQPKEVRYEALQTLCSAPPSDVLSCENWTTLCEKLTTSLSDPDPMFTDRILKFYAQTFTLSPLHMTKEIYTSLAKYLEVYFLSRENHLPTLSTGVDITSPNVTRLLKKVRLLNEYQKEAPSFWIRHPEKYMEEIVESTLSLLSVKHEQSHLVPQKILDPIYFFALVDTKAVWFKKWMHAYYSRTAVLRLLEKKYKCLITTAVQQCVQYLELCEAMKADEILRHPKHCGTKQKSFYYSGQELQYIYFIHSLCLLGRLLIYTQGRKLFPIKLKNRKDSVSLTNLLVLFTQLIYYSPSCPKMTSIMCSENYSPASMVTDVLRMLCDQKECAVECLYNSTVTEALLLPIHNLTKGTAAAPDCSETALIHIADILARIASVEEGLILLLYGENMNSSEEESLTGAHIIAKFSKKLLEEDISIFSGSEMLPVVKGAFISVCRQIYGTCEGLQVLLPYGLHESIAKAWKKTSLLSERIPTPVEGSDSVSSVSQVSPNSVAWEDNLLDDLLNFAATPKGLLLLQRTGAINECVTFMLSQYAKKPQVNRQKKFGYEVLVVQVASTAAGAVALQNSGFISALITELWSNLECGRDDVRLTHPRATPVDPIDRSCQKSFLALVNLLSYPAVYELTANQELPNKAEYSLREVPTCIIDIMDRLIVLNSEAKIRSLLNYEQSHTFGLRLLSVVCCDLDALLLLEAQYQVSNMLLHAQEENTFEISENHRNFIIDGLSVERNHVLVRINLIGGPSERILPPRMLEKGDDPYPWPMFSSYPLPHCYQSEGPRSADLKQDNDIGNLLSCFKMSDKQTEWIENCRRQFCKTMKSKPDAVHGSALGELLEKFVLLLTENPSECYFPSVEYTATDANVKNESLSSVQQLGMKMTVRYGRFLNLLKDGAENELALVLKHCEKFLKQQQSPVTSSLLCLQGNYAGHDWFVSSLFMIMLGDKGKTFHFLQHFSRLLTSAFLWVPRLHNSRYLPVDTLGTGIHPIYFCSAHYIEMLLKAEVPLVFSAFHMSGFAPSQICLQWITQCFWNYLDWIEICHYIATCVVLGPDYQVYVCIAVLKHLQRDILQHTQTQDLQVFLKEEALHGFRVSNYFEYMENLEQNYRPVLLRDMRSIRVQNT

Target ORF information:

RefSeq Version	NM_001033385.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus TBC1 domain family, member 32 (Tbc1d32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033385.3

ORF Insert Sequence:

ATGGCCCATT TCTCCAGCGA GGATGAGGTG ATGCTGCAGG CGATGCTCCG GCAGCTGTTC 
CAGAGCGTGA AGGAGAAAAT CACCGGAGCT CCCTCCTTGG AGTGCGCAGA GGAGATCCTC 
TTGCGGCTGG AGGAGACTGA CGAAAATTTT CACAACTATG AATTTGTGAA ATACCTCAGA 
CAGCACATAT GTAACATGTT GGGGTCTATG ATTGAAGAAG AAATGGAAAA ATGCACATCT 
GATCAGAATC AGGGGGAGGA TTCTGGCTAT GACACGGTTG TTCAGCATGT TACCAAAAGA 
ACGCAGGAAT CTAAAGAGTA TAAGGAAATG ATGCATTCCC TGAAGAACAT TATGATGGTG 
GTGGTCGAGG CGATGATCAA CAAGTTTGAG GAAGACGAGA CACGGAGTGA GGACAGGCAG 
AGGAAAATGC AGAGTGGCAG CTGCTGCACA GACAACTGCT CCGACAGTGA CTCCTCCTTC 
AATCAGAGTT ACAAATTCTG TCAAGGGAAA CTACGGTTGA TTTTAGACCA GTTGGATCCT 
GGCCAACCTA AAGAGGTGAG ATATGAAGCT TTACAAACAT TGTGCTCAGC TCCCCCATCC 
GACGTCCTGA GCTGTGAGAA CTGGACCACT CTCTGTGAAA AGCTGACCAC ATCTCTGTCT 
GATCCAGATC CTATGTTCAC TGACCGGATT TTAAAGTTCT ACGCACAGAC TTTTACCCTC 
TCACCGTTAC ATATGACCAA GGAGATTTAT ACAAGCTTAG CTAAATATCT GGAGGTATAT 
TTTCTTTCTA GAGAAAATCA CCTTCCTACT CTTTCAACTG GAGTAGACAT AACCAGTCCC 
AATGTGACCC GCTTACTGAA AAAGGTTCGC CTTCTGAATG AGTACCAGAA AGAGGCTCCA 
TCTTTTTGGA TTCGTCACCC AGAGAAGTAT ATGGAGGAAA TTGTGGAGAG TACTCTGTCC 
TTGCTCTCAG TCAAACACGA GCAAAGCCAC CTGGTGCCAC AGAAGATTTT GGATCCCATC 
TATTTTTTCG CATTAGTTGA TACTAAGGCT GTGTGGTTCA AAAAGTGGAT GCATGCATAT 
TACAGCAGAA CTGCAGTACT GAGACTTCTT GAAAAGAAAT ATAAATGTCT GATAACTACA 
GCAGTTCAGC AATGTGTTCA GTACTTGGAG TTATGTGAAG CTATGAAAGC TGATGAAATT 
CTGAGACATC CAAAACATTG TGGAACCAAG CAAAAGAGTT TCTACTATTC AGGACAAGAA 
TTGCAATATA TTTATTTCAT TCACTCACTG TGCCTTTTAG GAAGATTGTT GATCTATACA 
CAGGGCAGGA AATTATTCCC CATAAAGCTG AAGAATAGAA AAGATTCAGT ATCCCTGACA 
AATCTGCTGG TTTTGTTTAC TCAACTTATC TATTATTCAC CAAGTTGTCC AAAGATGACC 
TCCATCATGT GTTCAGAGAA CTATTCTCCT GCAAGTATGG TGACTGACGT TCTGCGGATG 
CTCTGCGACC AGAAAGAATG TGCAGTCGAG TGCTTATACA ACAGCACAGT GACAGAGGCG 
CTTCTCCTGC CCATTCATAA CCTGACGAAA GGAACTGCGG CTGCTCCAGA TTGCTCTGAG 
ACAGCTCTAA TTCATATAGC TGATATTTTG GCAAGAATCG CTTCTGTCGA GGAAGGACTC 
ATCTTACTTC TGTATGGAGA AAATATGAAC TCTTCTGAAG AAGAAAGTCT TACAGGTGCT 
CATATAATAG CCAAGTTTTC AAAGAAACTT CTTGAAGAAG ATATTTCTAT TTTTTCTGGA 
TCGGAAATGT TGCCTGTAGT TAAAGGAGCT TTTATTTCTG TGTGTCGTCA GATCTACGGT 
ACCTGCGAAG GCTTACAGGT GTTGCTTCCT TATGGTTTAC ATGAATCCAT AGCAAAGGCA 
TGGAAGAAGA CAAGCTTGCT ATCAGAAAGG ATCCCCACTC CAGTAGAGGG CTCTGATTCT 
GTTTCCTCAG TGAGCCAGGT GTCTCCGAAC AGTGTGGCTT GGGAAGACAA TTTGTTGGAT 
GATTTACTGA ATTTTGCAGC CACCCCCAAA GGACTGCTGC TTCTTCAGAG AACAGGCGCC 
ATCAATGAAT GTGTGACGTT TATGCTCAGC CAGTATGCAA AAAAGCCACA GGTCAACAGG 
CAGAAGAAGT TTGGCTATGA AGTCTTGGTT GTACAGGTGG CATCGACAGC AGCAGGTGCA 
GTGGCTCTAC AGAATTCAGG CTTTATTAGT GCACTTATAA CTGAGTTATG GTCCAACCTT 
GAATGTGGAA GAGATGATGT AAGGCTAACT CATCCCAGAG CTACCCCAGT GGACCCAATT 
GACCGAAGCT GTCAGAAGTC CTTCCTTGCA CTGGTGAACC TGCTGTCCTA CCCTGCTGTG 
TATGAGCTAA CAGCCAATCA GGAGCTTCCT AATAAGGCAG AGTATTCTCT TCGTGAAGTT 
CCCACATGCA TCATCGATAT AATGGATAGG CTTATTGTTT TGAACTCTGA AGCCAAGATC 
CGGTCTTTAC TGAATTATGA ACAGTCGCAC ACCTTTGGTC TAAGGTTACT AAGTGTGGTG 
TGCTGTGATC TGGATGCGCT TCTCTTACTG GAGGCTCAGT ATCAAGTATC TAACATGTTA 
TTACATGCTC AAGAAGAAAA TACCTTTGAG ATCTCTGAGA ACCACAGGAA CTTTATAATT 
GATGGATTAT CAGTAGAGAG AAATCATGTT CTCGTAAGGA TTAATCTTAT TGGTGGTCCT 
TCAGAGCGGA TATTGCCTCC AAGGATGCTT GAGAAGGGGG ATGACCCATA TCCTTGGCCA 
ATGTTTTCTT CATATCCATT GCCACACTGC TATCAGTCAG AAGGTCCACG AAGTGCTGAT 
CTAAAACAAG ACAATGATAT TGGAAACCTG TTATCATGCT TCAAAATGTC CGATAAACAA 
ACAGAGTGGA TAGAGAACTG CCGAAGACAG TTTTGTAAAA CAATGAAGTC CAAGCCTGAT 
GCAGTCCATG GAAGTGCCTT AGGAGAACTA CTGGAAAAAT TTGTGCTGCT TCTCACCGAA 
AACCCATCTG AATGTTACTT CCCTTCAGTG GAATACACAG CTACTGATGC AAATGTGAAG 
AATGAAAGTC TCTCATCTGT GCAGCAGCTT GGCATGAAAA TGACTGTCAG GTATGGGAGG 
TTTCTCAATT TGTTAAAAGA TGGTGCAGAA AATGAGCTTG CTTTGGTCTT AAAGCATTGT 
GAGAAATTCC TGAAACAACA GCAGTCACCG GTCACTTCTT CTCTCCTGTG CCTGCAAGGG 
AACTACGCAG GCCATGACTG GTTTGTGTCT TCTCTGTTCA TGATAATGCT GGGAGACAAA 
GGGAAGACCT TCCACTTTCT CCAGCATTTC TCCAGGCTGC TGACCTCTGC CTTCCTTTGG 
GTGCCACGGC TGCATAATTC TAGGTACCTT CCTGTCGACA CCCTAGGGAC CGGCATCCAT 
CCCATCTACT TTTGCAGTGC TCACTACATT GAAATGCTGC TGAAGGCTGA GGTGCCGCTT 
GTGTTTTCGG CTTTCCACAT GTCTGGTTTT GCTCCATCCC AGATTTGCCT GCAGTGGATA 
ACTCAGTGTT TTTGGAATTA CTTAGATTGG ATAGAAATAT GCCACTATAT TGCTACTTGT 
GTTGTCCTTG GTCCTGATTA TCAAGTGTAT GTCTGCATAG CCGTTCTCAA ACATCTGCAG 
CGAGATATTC TCCAGCATAC CCAGACTCAA GATCTGCAAG TCTTTCTCAA AGAAGAAGCA 
CTGCATGGGT TTCGAGTGAG CAACTATTTT GAATACATGG AGAATTTGGA GCAAAACTAC 
CGACCAGTGC TGCTGCGAGA CATGCGCAGC ATCAGAGTGC AGAACACATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbc1d32 ( XM_011243191.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241493.1 Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X1, mRNA.

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CloneID	OMu75298
Clone ID Related Accession (Same CDS sequence)	XM_011243191.3 , XM_011243191.2
Accession Version	XM_011243191.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein broad-minded isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATT TCTCCAGCGA GGATGAGGTG ATGCTGCAGG CGATGCTCCG GCAGCTGTTC 
CAGAGCGTGA AGGAGAAAAT CACCGGAGCT CCCTCCTTGG AGTGCGCAGA GGAGATCCTC 
TTGCGGCTGG AGGAGACTGA CGAAAATTTT CACAACTATG AATTTGTGAA ATACCTCAGA 
CAGCACATAT GTAACATGTT GGGGTCTATG ATTGAAGAAG AAATGGAAAA ATGCACATCT 
GATCAGAATC AGGGGGAGGA TTCTGGCTAT GACACGGTTG TTCAGCATGT TACCAAAAGA 
ACGCAGGAAT CTAAAGAGTA TAAGGAAATG ATGCATTCCC TGAAGAACAT TATGATGGTG 
GTGGTCGAGG CGATGATCAA CAAGTTTGAG GAAGACGAGA CACGGAGTGA GGACAGGCAG 
AGGAAAATGC AGAGTGGCAG CTGCTGCACA GACAACTGCT CCGACAGTGA CTCCTCCTTC 
AATCAGAGTT ACAAATTCTG TCAAGGGAAA CTACGGTTGA TTTTAGACCA GTTGGATCCT 
GGCCAACCTA AAGAGGTGAG ATATGAAGCT TTACAAACAT TGTGCTCAGC TCCCCCATCC 
GACGTCCTGA GCTGTGAGAA CTGGACCACT CTCTGTGAAA AGCTGACCAC ATCTCTGTCT 
GATCCAGATC CTATGTTCAC TGACCGGATT TTAAAGTTCT ACGCACAGAC TTTTACCCTC 
TCACCGTTAC ATATGACCAA GGAGATTTAT ACAAGCTTAG CTAAATATCT GGAGGTATAT 
TTTCTTTCTA GAGAAAATCA CCTTCCTACT CTTTCAACTG GAGTAGACAT AACCAGTCCC 
AATGTGACCC GCTTACTGAA AAAGGTTCGC CTTCTGAATG AGTACCAGAA AGAGGCTCCA 
TCTTTTTGGA TTCGTCACCC AGAGAAGTAT ATGGAGGAAA TTGTGGAGAG TACTCTGTCC 
TTGCTCTCAG TCAAACACGA GCAAAGCCAC CTGGTGCCAC AGAAGATTTT GGATCCCATC 
TATTTTTTCG CATTAGTTGA TACTAAGGCT GTGTGGTTCA AAAAGTGGAT GCATGCATAT 
TACAGCAGAA CTGCAGTACT GAGACTTCTT GAAAAGAAAT ATAAATGTCT GATAACTACA 
GCAGTTCAGC AATGTGTTCA GTACTTGGAG TTATGTGAAG CTATGAAAGC TGATGAAATT 
CTGAGACATC CAAAACATTG TGGAACCAAG CAAAAGAGTT TCTACTATTC AGGACAAGAA 
TTGCAATATA TTTATTTCAT TCACTCACTG TGCCTTTTAG GAAGATTGTT GATCTATACA 
CAGGGCAGGA AATTATTCCC CATAAAGCTG AAGAATAGAA AAGATTCAGT ATCCCTGACA 
AATCTGCTGG TTTTGTTTAC TCAACTTATC TATTATTCAC CAAGTTGTCC AAAGATGACC 
TCCATCATGT GTTCAGAGAA CTATTCTCCT GCAAGTATGG TGACTGACGT TCTGCGGATG 
CTCTGCGACC AGAAAGAATG TGCAGTCGAG TGCTTATACA ACAGCACAGT GACAGAGGCG 
CTTCTCCTGC CCATTCATAA CCTGACGAAA GGAACTGCGG CTGCTCCAGA TTGCTCTGAG 
ACAGCTCTAA TTCATATAGC TGATATTTTG GCAAGAATCG CTTCTGTCGA GGAAGGACTC 
ATCTTACTTC TGTATGGAGA AAATATGAAC TCTTCTGAAG AAGAAAGTCT TACAGGTGCT 
CATATAATAG CCAAGTTTTC AAAGAAACTT CTTGAAGAAG ATATTTCTAT TTTTTCTGGA 
TCGGAAATGT TGCCTGTAGT TAAAGGAGCT TTTATTTCTG TGTGTCGTCA GATCTACGGT 
ACCTGCGAAG GCTTACAGGT GTTGCTTCCT TATGGTTTAC ATGAATCCAT AGCAAAGGCA 
TGGAAGAAGA CAAGCTTGCT ATCAGAAAGG ATCCCCACTC CAGTAGAGGG CTCTGATTCT 
GTTTCCTCAG TGAGCCAGGT GTCTCCGAAC AGTGTGGCTT GGGAAGACAA TTTGTTGGAT 
GATTTACTGA ATTTTGCAGC CACCCCCAAA GGACTGCTGC TTCTTCAGAG AACAGGCGCC 
ATCAATGAAT GTGTGACGTT TATGCTCAGC CAGTATGCAA AAAAGCCACA GGTCAACAGG 
CAGAAGAAGT TTGGCTATGA AGTCTTGGTT GTACAGGTGG CATCGACAGC AGCAGGTGCA 
GTGGCTCTAC AGAATTCAGG CTTTATTAGT GCACTTATAA CTGAGTTATG GTCCAACCTT 
GAATGTGGAA GAGATGATGT AAGGCTAACT CATCCCAGAG CTACCCCAGT GGACCCAATT 
GACCGAAGCT GTCAGAAGTC CTTCCTTGCA CTGGTGAACC TGCTGTCCTA CCCTGCTGTG 
TATGAGCTAA CAGCCAATCA GGAGCTTCCT AATAAGGCAG AGTATTCTCT TCGTGAAGTT 
CCCACATGCA TCATCGATAT AATGGATAGG CTTATTGTTT TGAACTCTGA AGCCAAGATC 
CGGTCTTTAC TGAATTATGA ACAGTCGCAC ACCTTTGGTC TAAGGTTACT AAGTGTGGTG 
TGCTGTGATC TGGATGCGCT TCTCTTACTG GAGGCTCAGT ATCAAGTATC TAACATGTTA 
TTACATGCTC AAGAAGAAAA TACCTTTGAG ATCTCTGAGA ACCACAGGAA CTTTATAATT 
GATGGATTAT CAGTAGAGAG AAATCATGTT CTCGTAAGGA TTAATCTTAT TGGTGGTCCT 
TCAGAGCGGA TATTGCCTCC AAGGATGCTT GAGAAGGGGG ATGACCCATA TCCTTGGCCA 
ATGTTTTCTT CATATCCATT GCCACACTGC TATCAGTCAG AAGGTCCACG AAGTGCTGAT 
CTAAAACAAG ACAATGATAT TGGAAACCTG TTATCATGCT TCAAAATGTC CGATAAACAA 
ACAGAGTGGA TAGAGAACTG CCGAAGACAG TTTTGTAAAA CAATGAAGTC CAAGCCTGAT 
GCAGTCCATG GAAGTGCCTT AGGAGAACTA CTGGAAAAAT TTGTGCTGCT TCTCACCGAA 
AACCCATCTG AATGTTACTT CCCTTCAGTG GAATACACAG GTGTGTTGCC TGAGTGTACC 
ACATGTCTAT GTACCACATA TGTTCCTGGT ACTCACAGAG GCCAGAAGAA GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241493.1
CDS	826..4002
Translation	MAHFSSEDEVMLQAMLRQLFQSVKEKITGAPSLECAEEILLRLEETDENFHNYEFVKYLRQHICNMLGSMIEEEMEKCTSDQNQGEDSGYDTVVQHVTKRTQESKEYKEMMHSLKNIMMVVVEAMINKFEEDETRSEDRQRKMQSGSCCTDNCSDSDSSFNQSYKFCQGKLRLILDQLDPGQPKEVRYEALQTLCSAPPSDVLSCENWTTLCEKLTTSLSDPDPMFTDRILKFYAQTFTLSPLHMTKEIYTSLAKYLEVYFLSRENHLPTLSTGVDITSPNVTRLLKKVRLLNEYQKEAPSFWIRHPEKYMEEIVESTLSLLSVKHEQSHLVPQKILDPIYFFALVDTKAVWFKKWMHAYYSRTAVLRLLEKKYKCLITTAVQQCVQYLELCEAMKADEILRHPKHCGTKQKSFYYSGQELQYIYFIHSLCLLGRLLIYTQGRKLFPIKLKNRKDSVSLTNLLVLFTQLIYYSPSCPKMTSIMCSENYSPASMVTDVLRMLCDQKECAVECLYNSTVTEALLLPIHNLTKGTAAAPDCSETALIHIADILARIASVEEGLILLLYGENMNSSEEESLTGAHIIAKFSKKLLEEDISIFSGSEMLPVVKGAFISVCRQIYGTCEGLQVLLPYGLHESIAKAWKKTSLLSERIPTPVEGSDSVSSVSQVSPNSVAWEDNLLDDLLNFAATPKGLLLLQRTGAINECVTFMLSQYAKKPQVNRQKKFGYEVLVVQVASTAAGAVALQNSGFISALITELWSNLECGRDDVRLTHPRATPVDPIDRSCQKSFLALVNLLSYPAVYELTANQELPNKAEYSLREVPTCIIDIMDRLIVLNSEAKIRSLLNYEQSHTFGLRLLSVVCCDLDALLLLEAQYQVSNMLLHAQEENTFEISENHRNFIIDGLSVERNHVLVRINLIGGPSERILPPRMLEKGDDPYPWPMFSSYPLPHCYQSEGPRSADLKQDNDIGNLLSCFKMSDKQTEWIENCRRQFCKTMKSKPDAVHGSALGELLEKFVLLLTENPSECYFPSVEYTGVLPECTTCLCTTYVPGTHRGQKKA

Target ORF information:

RefSeq Version	XM_011243191.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TBC1 domain family, member 32 (Tbc1d32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243191.2

ORF Insert Sequence:

ATGGCCCATT TCTCCAGCGA GGATGAGGTG ATGCTGCAGG CGATGCTCCG GCAGCTGTTC 
CAGAGCGTGA AGGAGAAAAT CACCGGAGCT CCCTCCTTGG AGTGCGCAGA GGAGATCCTC 
TTGCGGCTGG AGGAGACTGA CGAAAATTTT CACAACTATG AATTTGTGAA ATACCTCAGA 
CAGCACATAT GTAACATGTT GGGGTCTATG ATTGAAGAAG AAATGGAAAA ATGCACATCT 
GATCAGAATC AGGGGGAGGA TTCTGGCTAT GACACGGTTG TTCAGCATGT TACCAAAAGA 
ACGCAGGAAT CTAAAGAGTA TAAGGAAATG ATGCATTCCC TGAAGAACAT TATGATGGTG 
GTGGTCGAGG CGATGATCAA CAAGTTTGAG GAAGACGAGA CACGGAGTGA GGACAGGCAG 
AGGAAAATGC AGAGTGGCAG CTGCTGCACA GACAACTGCT CCGACAGTGA CTCCTCCTTC 
AATCAGAGTT ACAAATTCTG TCAAGGGAAA CTACGGTTGA TTTTAGACCA GTTGGATCCT 
GGCCAACCTA AAGAGGTGAG ATATGAAGCT TTACAAACAT TGTGCTCAGC TCCCCCATCC 
GACGTCCTGA GCTGTGAGAA CTGGACCACT CTCTGTGAAA AGCTGACCAC ATCTCTGTCT 
GATCCAGATC CTATGTTCAC TGACCGGATT TTAAAGTTCT ACGCACAGAC TTTTACCCTC 
TCACCGTTAC ATATGACCAA GGAGATTTAT ACAAGCTTAG CTAAATATCT GGAGGTATAT 
TTTCTTTCTA GAGAAAATCA CCTTCCTACT CTTTCAACTG GAGTAGACAT AACCAGTCCC 
AATGTGACCC GCTTACTGAA AAAGGTTCGC CTTCTGAATG AGTACCAGAA AGAGGCTCCA 
TCTTTTTGGA TTCGTCACCC AGAGAAGTAT ATGGAGGAAA TTGTGGAGAG TACTCTGTCC 
TTGCTCTCAG TCAAACACGA GCAAAGCCAC CTGGTGCCAC AGAAGATTTT GGATCCCATC 
TATTTTTTCG CATTAGTTGA TACTAAGGCT GTGTGGTTCA AAAAGTGGAT GCATGCATAT 
TACAGCAGAA CTGCAGTACT GAGACTTCTT GAAAAGAAAT ATAAATGTCT GATAACTACA 
GCAGTTCAGC AATGTGTTCA GTACTTGGAG TTATGTGAAG CTATGAAAGC TGATGAAATT 
CTGAGACATC CAAAACATTG TGGAACCAAG CAAAAGAGTT TCTACTATTC AGGACAAGAA 
TTGCAATATA TTTATTTCAT TCACTCACTG TGCCTTTTAG GAAGATTGTT GATCTATACA 
CAGGGCAGGA AATTATTCCC CATAAAGCTG AAGAATAGAA AAGATTCAGT ATCCCTGACA 
AATCTGCTGG TTTTGTTTAC TCAACTTATC TATTATTCAC CAAGTTGTCC AAAGATGACC 
TCCATCATGT GTTCAGAGAA CTATTCTCCT GCAAGTATGG TGACTGACGT TCTGCGGATG 
CTCTGCGACC AGAAAGAATG TGCAGTCGAG TGCTTATACA ACAGCACAGT GACAGAGGCG 
CTTCTCCTGC CCATTCATAA CCTGACGAAA GGAACTGCGG CTGCTCCAGA TTGCTCTGAG 
ACAGCTCTAA TTCATATAGC TGATATTTTG GCAAGAATCG CTTCTGTCGA GGAAGGACTC 
ATCTTACTTC TGTATGGAGA AAATATGAAC TCTTCTGAAG AAGAAAGTCT TACAGGTGCT 
CATATAATAG CCAAGTTTTC AAAGAAACTT CTTGAAGAAG ATATTTCTAT TTTTTCTGGA 
TCGGAAATGT TGCCTGTAGT TAAAGGAGCT TTTATTTCTG TGTGTCGTCA GATCTACGGT 
ACCTGCGAAG GCTTACAGGT GTTGCTTCCT TATGGTTTAC ATGAATCCAT AGCAAAGGCA 
TGGAAGAAGA CAAGCTTGCT ATCAGAAAGG ATCCCCACTC CAGTAGAGGG CTCTGATTCT 
GTTTCCTCAG TGAGCCAGGT GTCTCCGAAC AGTGTGGCTT GGGAAGACAA TTTGTTGGAT 
GATTTACTGA ATTTTGCAGC CACCCCCAAA GGACTGCTGC TTCTTCAGAG AACAGGCGCC 
ATCAATGAAT GTGTGACGTT TATGCTCAGC CAGTATGCAA AAAAGCCACA GGTCAACAGG 
CAGAAGAAGT TTGGCTATGA AGTCTTGGTT GTACAGGTGG CATCGACAGC AGCAGGTGCA 
GTGGCTCTAC AGAATTCAGG CTTTATTAGT GCACTTATAA CTGAGTTATG GTCCAACCTT 
GAATGTGGAA GAGATGATGT AAGGCTAACT CATCCCAGAG CTACCCCAGT GGACCCAATT 
GACCGAAGCT GTCAGAAGTC CTTCCTTGCA CTGGTGAACC TGCTGTCCTA CCCTGCTGTG 
TATGAGCTAA CAGCCAATCA GGAGCTTCCT AATAAGGCAG AGTATTCTCT TCGTGAAGTT 
CCCACATGCA TCATCGATAT AATGGATAGG CTTATTGTTT TGAACTCTGA AGCCAAGATC 
CGGTCTTTAC TGAATTATGA ACAGTCGCAC ACCTTTGGTC TAAGGTTACT AAGTGTGGTG 
TGCTGTGATC TGGATGCGCT TCTCTTACTG GAGGCTCAGT ATCAAGTATC TAACATGTTA 
TTACATGCTC AAGAAGAAAA TACCTTTGAG ATCTCTGAGA ACCACAGGAA CTTTATAATT 
GATGGATTAT CAGTAGAGAG AAATCATGTT CTCGTAAGGA TTAATCTTAT TGGTGGTCCT 
TCAGAGCGGA TATTGCCTCC AAGGATGCTT GAGAAGGGGG ATGACCCATA TCCTTGGCCA 
ATGTTTTCTT CATATCCATT GCCACACTGC TATCAGTCAG AAGGTCCACG AAGTGCTGAT 
CTAAAACAAG ACAATGATAT TGGAAACCTG TTATCATGCT TCAAAATGTC CGATAAACAA 
ACAGAGTGGA TAGAGAACTG CCGAAGACAG TTTTGTAAAA CAATGAAGTC CAAGCCTGAT 
GCAGTCCATG GAAGTGCCTT AGGAGAACTA CTGGAAAAAT TTGTGCTGCT TCTCACCGAA 
AACCCATCTG AATGTTACTT CCCTTCAGTG GAATACACAG GTGTGTTGCC TGAGTGTACC 
ACATGTCTAT GTACCACATA TGTTCCTGGT ACTCACAGAG GCCAGAAGAA GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cell Cycle-Related Kinase (CCRK) regulates ciliogenesis and Hedgehog signaling in mice.
PLoS genetics13(8)e1006912(2017 Aug)
Snouffer A,Brown D,Lee H,Walsh J,Lupu F,Norman R,Lechtreck K,Ko HW,Eggenschwiler J

Genetic link between renal birth defects and congenital heart disease.
Nature communications711103(2016 Mar)
San Agustin JT,Klena N,Granath K,Panigrahy A,Stewart E,Devine W,Strittmatter L,Jonassen JA,Liu X,Lo CW,Pazour GJ

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Nature521(7553)520-4(2015 May)
Li Y,Klena NT,Gabriel GC,Liu X,Kim AJ,Lemke K,Chen Y,Chatterjee B,Devine W,Damerla RR,Chang C,Yagi H,San Agustin JT,Thahir M,Anderton S,Lawhead C,Vescovi A,Pratt H,Morgan J,Haynes L,Smith CL,Eppig JT,Reinholdt L,Francis R,Leatherbury L,Ganapathiraju MK,Tobita K,Pazour GJ,Lo CW

Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction.
Developmental cell18(2)237-47(2010 Feb)
Ko HW,Norman RX,Tran J,Fuller KP,Fukuda M,Eggenschwiler JT

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Tbc1d32 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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