Ddx20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx20
Entrez Gene ID	53975
Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Synonyms	GEMIN3,dp103
General protein information	Preferred Names DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 Names probable ATP-dependent RNA helicase DDX20 DEAD box protein 20 DEAD box protein DP 103 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20 ROSF-1 component of gems 3 gemin-3 regulator of steroidogenic factor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_017397.3	NP_059093.3	probable ATP-dependent RNA helicase DDX20
XM_006501701.3	XP_006501764.1	probable ATP-dependent RNA helicase DDX20 isoform X1
XM_006501701.2	XP_006501764.1	probable ATP-dependent RNA helicase DDX20 isoform X1
XM_006501701.4	XP_006501764.1	probable ATP-dependent RNA helicase DDX20 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DDX20	NP_001095588.1
Pan troglodytes (chimpanzee)	DDX20	XP_003308378.1
Danio rerio (zebrafish)	ddx20	NP_001116795.1
Rattus norvegicus (Norway rat)	Ddx20	NP_001178640.1
Canis lupus familiaris (dog)	DDX20	XP_003639658.1
Mus musculus (house mouse)	Ddx20	NP_059093.3
Gallus gallus (chicken)	DDX20	XP_417996.1
Homo sapiens (human)	DDX20	NP_009135.4
Xenopus tropicalis (tropical clawed frog)	ddx20	XP_002932842.1

Gene Ontology
Bibliography

Related articles in PubMed

DEAD-box protein-103 (DP103, Ddx20) is essential for early embryonic development and modulates ovarian morphology and function.
Mouillet JF, Yan X, Ou Q, Jin L, Muglia LJ, Crawford PA, Sadovsky Y
Endocrinology149(5)2168-75(2008 May)

Transcriptional factor FOXL2 interacts with DP103 and induces apoptosis.
Lee K, Pisarska MD, Ko JJ, Kang Y, Yoon S, Ryou SM, Cha KY, Bae J
Biochemical and biophysical research communications336(3)876-81(2005 Oct)

Temporal and spatial transcriptional programs in murine kidney development.
Challen G, Gardiner B, Caruana G, Kostoulias X, Martinez G, Crowe M, Taylor DF, Bertram J, Little M, Grimmond SM
Physiological genomics23(2)159-71(2005 Oct)

The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification.
Lee MB, Lebedeva LA, Suzawa M, Wadekar SA, Desclozeaux M, Ingraham HA
Molecular and cellular biology25(5)1879-90(2005 Mar)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Diverse functions for murine DP103, with an obligatory role during early embryonic development and also in modulation of steroidogenesis.
Title: DEAD-box protein-103 (DP103, Ddx20) is essential for early embryonic development and modulates ovarian morphology and function.

DP103 directly interacts with sumoylated SF-1 and is coupled to transcriptional repression by protein sumoylation.
Title: The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification.

Ddx20 repressed Egr2-mediated transcriptional activation with significant promoter specificity
Title: The Ddx20/DP103 dead box protein represses transcriptional activation by Egr2/Krox-20.

The following Ddx20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10754	NM_017397.3 Latest version!	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu10754	XM_006501701.4 Latest version!	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu10754	XM_006501701.2	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu10754	XM_006501701.3	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx20 ( NM_017397.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_059093.3 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), mRNA.

Price & Availability↑

CloneID	OMu10754
Clone ID Related Accession (Same CDS sequence)	NM_017397.3 , XM_006501701.3 , XM_006501701.2 , XM_006501701.4
Accession Version	NM_017397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125471.1. On Jun 3, 2007 this sequence version replaced NM_017397.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC125471.1, AF220454.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059093.3
CDS	145..2622
Translation	MAAAAFEVPAALTTSESTMAAERAAAPVQAVEPTPASPWTQRTAHDIGGPRTRTGDVVLAEPADFESLLLSRPVLEGLRAAGFERPSPVQLKAIPLGRCGLDLIVQAKSGTGKTCVFSTIALDSLILENYSTQILILAPTREIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPGRIKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQMLAVSATYPEVLANALTRYMRDPTFVRLNPSDPSLIGLKQYYQVVNSYPLAHKIFEEKTQHLQELFSKVPFNQALVFSNLHSRAQHLADILSSKGFPTECISGNMNQNQRLDAMAKLKQFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLDWETYMHRIGRAGRFGTLGLTVTYCCRGEEENMMMKIAQKCNINLLPLPDPIPPGLMEECLNWDVEVKAAMHTYSSPTVATQSPKKQVQKLERAFQSQRTPGNQTPSPRNTSASALSARPKHSKPKLPVKSHSECGVLEKAAPPQESGCPAQLEEQVKNSVQTSVEDSSSNSQHQAKDSSPGSLPKIPCLSSFKVHQPSTLTFAELVDDYEHYIKEGLEKPVEIIRHYTGPEAQTGNPQNGFVRNRVSEDRAQMLVSSSQSGDSESDSDSCSSRTSSQSKGNKSYLEGSSDTQLKDTECTPVGGPLSLEQVQNGNDTPTQVEYQEAPETQVKARHKEGANQRSKQSRRNPARRSSYRVQSEPQEESWYDCHRETTASFSDTYQDYEEYWRAYYRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTVYLQEMMRGNQ

Target ORF information:

RefSeq Version	NM_017397.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017397.3

ORF Insert Sequence:

ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx20 ( XM_006501701.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501764.1 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu10754
Clone ID Related Accession (Same CDS sequence)	NM_017397.3 , XM_006501701.3 , XM_006501701.2 , XM_006501701.4
Accession Version	XM_006501701.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501764.1
CDS	518..2995
Translation	MAAAAFEVPAALTTSESTMAAERAAAPVQAVEPTPASPWTQRTAHDIGGPRTRTGDVVLAEPADFESLLLSRPVLEGLRAAGFERPSPVQLKAIPLGRCGLDLIVQAKSGTGKTCVFSTIALDSLILENYSTQILILAPTREIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPGRIKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQMLAVSATYPEVLANALTRYMRDPTFVRLNPSDPSLIGLKQYYQVVNSYPLAHKIFEEKTQHLQELFSKVPFNQALVFSNLHSRAQHLADILSSKGFPTECISGNMNQNQRLDAMAKLKQFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLDWETYMHRIGRAGRFGTLGLTVTYCCRGEEENMMMKIAQKCNINLLPLPDPIPPGLMEECLNWDVEVKAAMHTYSSPTVATQSPKKQVQKLERAFQSQRTPGNQTPSPRNTSASALSARPKHSKPKLPVKSHSECGVLEKAAPPQESGCPAQLEEQVKNSVQTSVEDSSSNSQHQAKDSSPGSLPKIPCLSSFKVHQPSTLTFAELVDDYEHYIKEGLEKPVEIIRHYTGPEAQTGNPQNGFVRNRVSEDRAQMLVSSSQSGDSESDSDSCSSRTSSQSKGNKSYLEGSSDTQLKDTECTPVGGPLSLEQVQNGNDTPTQVEYQEAPETQVKARHKEGANQRSKQSRRNPARRSSYRVQSEPQEESWYDCHRETTASFSDTYQDYEEYWRAYYRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTVYLQEMMRGNQ

Target ORF information:

RefSeq Version	XM_006501701.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501701.3

ORF Insert Sequence:

ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx20 ( XM_006501701.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501764.1 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.

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CloneID	OMu10754
Clone ID Related Accession (Same CDS sequence)	NM_017397.3 , XM_006501701.3 , XM_006501701.2 , XM_006501701.4
Accession Version	XM_006501701.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	probable ATP-dependent RNA helicase DDX20 isoform X1
Comment

ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006501764.1

Translation

MAAAAFEVPAALTTSESTMAAERAAAPVQAVEPTPASPWTQRTAHDIGGPRTRTGDVVLAEPADFESLLLSRPVLEGLRAAGFERPSPVQLKAIPLGRCGLDLIVQAKSGTGKTCVFSTIALDSLILENYSTQILILAPTREIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPGRIKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQMLAVSATYPEVLANALTRYMRDPTFVRLNPSDPSLIGLKQYYQVVNSYPLAHKIFEEKTQHLQELFSKVPFNQALVFSNLHSRAQHLADILSSKGFPTECISGNMNQNQRLDAMAKLKQFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLDWETYMHRIGRAGRFGTLGLTVTYCCRGEEENMMMKIAQKCNINLLPLPDPIPPGLMEECLNWDVEVKAAMHTYSSPTVATQSPKKQVQKLERAFQSQRTPGNQTPSPRNTSASALSARPKHSKPKLPVKSHSECGVLEKAAPPQESGCPAQLEEQVKNSVQTSVEDSSSNSQHQAKDSSPGSLPKIPCLSSFKVHQPSTLTFAELVDDYEHYIKEGLEKPVEIIRHYTGPEAQTGNPQNGFVRNRVSEDRAQMLVSSSQSGDSESDSDSCSSRTSSQSKGNKSYLEGSSDTQLKDTECTPVGGPLSLEQVQNGNDTPTQVEYQEAPETQVKARHKEGANQRSKQSRRNPARRSSYRVQSEPQEESWYDCHRETTASFSDTYQDYEEYWRAYYRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTVYLQEMMRGNQ

Target ORF information:

RefSeq Version	XM_006501701.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501701.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx20 ( XM_006501701.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501764.1 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu10754
Clone ID Related Accession (Same CDS sequence)	NM_017397.3 , XM_006501701.3 , XM_006501701.2 , XM_006501701.4
Accession Version	XM_006501701.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501701.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501764.1
CDS	188..2665
Translation	MAAAAFEVPAALTTSESTMAAERAAAPVQAVEPTPASPWTQRTAHDIGGPRTRTGDVVLAEPADFESLLLSRPVLEGLRAAGFERPSPVQLKAIPLGRCGLDLIVQAKSGTGKTCVFSTIALDSLILENYSTQILILAPTREIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPGRIKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQMLAVSATYPEVLANALTRYMRDPTFVRLNPSDPSLIGLKQYYQVVNSYPLAHKIFEEKTQHLQELFSKVPFNQALVFSNLHSRAQHLADILSSKGFPTECISGNMNQNQRLDAMAKLKQFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLDWETYMHRIGRAGRFGTLGLTVTYCCRGEEENMMMKIAQKCNINLLPLPDPIPPGLMEECLNWDVEVKAAMHTYSSPTVATQSPKKQVQKLERAFQSQRTPGNQTPSPRNTSASALSARPKHSKPKLPVKSHSECGVLEKAAPPQESGCPAQLEEQVKNSVQTSVEDSSSNSQHQAKDSSPGSLPKIPCLSSFKVHQPSTLTFAELVDDYEHYIKEGLEKPVEIIRHYTGPEAQTGNPQNGFVRNRVSEDRAQMLVSSSQSGDSESDSDSCSSRTSSQSKGNKSYLEGSSDTQLKDTECTPVGGPLSLEQVQNGNDTPTQVEYQEAPETQVKARHKEGANQRSKQSRRNPARRSSYRVQSEPQEESWYDCHRETTASFSDTYQDYEEYWRAYYRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTVYLQEMMRGNQ

Target ORF information:

RefSeq Version	XM_006501701.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501701.4

ORF Insert Sequence:

ATGGCGGCGG CGGCGTTTGA AGTCCCGGCG GCCTTGACAA CCTCCGAGTC CACTATGGCT 
GCGGAGCGTG CGGCCGCGCC GGTGCAGGCT GTGGAGCCGA CTCCGGCATC CCCGTGGACC 
CAGCGGACCG CACACGACAT CGGCGGGCCG CGCACACGTA CCGGGGACGT GGTGCTGGCG 
GAGCCCGCGG ACTTCGAGTC GCTGCTGCTG TCGCGACCGG TGCTGGAGGG TCTGCGGGCG 
GCCGGCTTCG AGAGACCCTC TCCGGTGCAG CTCAAGGCCA TCCCGTTGGG GCGCTGCGGG 
CTCGATTTAA TTGTCCAAGC TAAGTCGGGC ACTGGAAAAA CTTGTGTATT CTCCACCATT 
GCTCTGGACT CTCTGATTCT TGAAAACTAT AGTACTCAGA TTTTGATATT GGCTCCCACA 
AGAGAAATTG CTGTTCAGAT ACACTCGGTT ATCACAGCCA TTGGAATAAA AATGGAAGGA 
TTAGAATGTC ATGTCTTTAT TGGAGGGACT CCATTATCAC AAGACAAAAC CAGACTTAAA 
AAGTGTCATA TTGCTGTTGG ATCTCCTGGT AGAATTAAAC AGCTCATAGA ACTTGACTAC 
CTGAATCCGG GCAGTATCCG TCTCTTTATT CTTGATGAAG CAGATAAGCT TTTAGAAGAA 
GGCAGTTTCC AGGAGCAAAT AAATTGGATT TACTCCTCTT TACCTGCGAG TAAGCAGATG 
TTGGCAGTAT CTGCTACTTA CCCTGAAGTT TTGGCTAATG CTTTGACAAG GTATATGAGA 
GATCCCACTT TTGTAAGATT GAATCCCAGC GATCCAAGTC TCATAGGTTT GAAGCAGTAC 
TACCAAGTTG TCAATTCTTA CCCTTTGGCT CATAAGATTT TTGAGGAAAA GACTCAGCAC 
TTACAGGAAC TTTTTAGTAA AGTCCCATTT AATCAAGCCT TGGTGTTTTC TAATTTGCAC 
AGCAGAGCTC AACATTTGGC TGATATCCTT TCTTCTAAAG GTTTTCCTAC TGAATGCATT 
TCAGGTAACA TGAATCAGAA TCAGCGCCTT GATGCTATGG CTAAACTGAA GCAGTTTCAT 
TGCAGAGTCC TCATTTCTAC AGACTTGACG TCCCGTGGGA TTGACGCAGA GAAGGTGAAT 
CTAGTTGTAA ATCTCGATGT GCCGTTAGAC TGGGAGACTT ACATGCATCG GATTGGCAGA 
GCTGGCCGTT TTGGTACATT GGGACTGACA GTGACCTACT GTTGTAGAGG AGAAGAAGAA 
AATATGATGA TGAAAATTGC TCAGAAATGT AATATCAACC TTCTTCCTTT ACCAGATCCT 
ATTCCTCCTG GCCTGATGGA AGAATGCTTG AATTGGGATG TGGAAGTTAA AGCTGCCATG 
CATACTTACA GCTCACCAAC TGTAGCTACT CAGTCCCCCA AAAAGCAAGT TCAAAAACTA 
GAGAGAGCCT TCCAGTCTCA AAGAACGCCT GGGAACCAAA CGCCTTCCCC TAGAAATACT 
TCTGCATCTG CACTGTCAGC CAGGCCAAAG CACAGCAAAC CAAAGCTTCC TGTGAAAAGC 
CACTCAGAGT GTGGAGTCCT AGAAAAGGCA GCTCCACCAC AAGAGTCGGG CTGTCCCGCA 
CAGCTGGAAG AGCAAGTTAA GAATTCTGTT CAGACCTCTG TTGAGGATTC CTCCAGTAAC 
AGCCAGCATC AGGCCAAAGA TAGCTCACCT GGGTCACTCC CTAAAATCCC TTGTCTGTCT 
TCTTTTAAAG TCCATCAGCC GTCTACTTTG ACTTTTGCAG AACTGGTAGA CGATTATGAG 
CACTATATTA AAGAGGGGTT AGAGAAACCT GTGGAAATTA TCCGGCACTA CACAGGGCCT 
GAGGCCCAGA CCGGGAATCC TCAGAATGGC TTTGTGAGAA ATAGAGTTTC TGAAGATAGA 
GCACAGATGT TGGTGAGTAG TAGCCAGTCT GGAGACTCAG AGAGTGACAG CGATTCTTGC 
AGCTCAAGGA CTTCTTCCCA GAGCAAAGGT AATAAGTCTT ACTTGGAAGG CTCCTCAGAC 
ACTCAGTTGA AAGACACTGA ATGTACTCCT GTGGGTGGCC CTCTCTCTCT GGAACAAGTT 
CAGAATGGAA ATGATACCCC GACTCAAGTG GAGTATCAAG AAGCCCCTGA AACCCAGGTA 
AAGGCAAGGC ATAAAGAAGG CGCTAACCAA AGGTCTAAGC AAAGTCGGAG AAACCCAGCA 
CGGCGTTCTT CTTACCGAGT ACAGTCAGAA CCCCAGGAAG AGAGTTGGTA TGACTGTCAC 
AGGGAAACAA CTGCAAGTTT TTCTGATACT TACCAGGATT ATGAGGAGTA CTGGAGAGCG 
TATTACAGGG CATGGCAAGA GTATTATGCT GCTGCTTCCC ATTCATACTA TTGGAATGCT 
CAGAGACATC CAAGCTGGAT GGCTGCCTAT CATATGAATA CAGTTTATCT ACAAGAGATG 
ATGCGTGGTA ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DEAD-box protein-103 (DP103, Ddx20) is essential for early embryonic development and modulates ovarian morphology and function.
Endocrinology149(5)2168-75(2008 May)
Mouillet JF,Yan X,Ou Q,Jin L,Muglia LJ,Crawford PA,Sadovsky Y

Transcriptional factor FOXL2 interacts with DP103 and induces apoptosis.
Biochemical and biophysical research communications336(3)876-81(2005 Oct)
Lee K,Pisarska MD,Ko JJ,Kang Y,Yoon S,Ryou SM,Cha KY,Bae J

Temporal and spatial transcriptional programs in murine kidney development.
Physiological genomics23(2)159-71(2005 Oct)
Challen G,Gardiner B,Caruana G,Kostoulias X,Martinez G,Crowe M,Taylor DF,Bertram J,Little M,Grimmond SM

The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification.
Molecular and cellular biology25(5)1879-90(2005 Mar)
Lee MB,Lebedeva LA,Suzawa M,Wadekar SA,Desclozeaux M,Ingraham HA

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Ddx20 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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