DDX20 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX20
Entrez Gene ID	11218
Full Name	DEAD-box helicase 20
Synonyms	DP103,GEMIN3
General protein information	Preferred Names DEAD-box helicase 20 Names probable ATP-dependent RNA helicase DDX20 DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 DEAD box protein 20 DEAD box protein DP 103 DEAD-box protein DP103 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD SMN-interacting protein component of gems 3 gemin-3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p13.2
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq, Jul 2008].
Disorder MIM:	606168

mRNA and Protein(s)

mRNA	Protein	Name
NM_007204.5	NP_009135.4	probable ATP-dependent RNA helicase DDX20
NM_007204.4	NP_009135.4	probable ATP-dependent RNA helicase DDX20

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DDX20	NP_001095588.1
Pan troglodytes (chimpanzee)	DDX20	XP_003308378.1
Danio rerio (zebrafish)	ddx20	NP_001116795.1
Rattus norvegicus (Norway rat)	Ddx20	NP_001178640.1
Canis lupus familiaris (dog)	DDX20	XP_003639658.1
Mus musculus (house mouse)	Ddx20	NP_059093.3
Gallus gallus (chicken)	DDX20	XP_417996.1
Homo sapiens (human)	DDX20	NP_009135.4
Xenopus tropicalis (tropical clawed frog)	ddx20	XP_002932842.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of the polymorphic variants of RAN and GEMIN3 genes and risk of Primary Open-Angle Glaucoma in the Polish population.
Molasy M, Walczak A, Przyby?owska-Sygut K, Zaleska-?mijewska A, Szaflik J, Szaflik JP, Majsterek I
Ophthalmic genetics1-9(2017 Nov)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

High expression of DDX20 enhances the proliferation and metastatic potential of prostate cancer cells through the NF-?B pathway.
Chen W, Zhou P, Li X
International journal of molecular medicine37(6)1551-7(2016 Jun)

A Survival Association Study of 102 Polymorphisms Previously Associated with Survival Outcomes in Colorectal Cancer.
Savas S, Xu J, Werdyani S, Shestopaloff K, Dicks E, Green J, Parfrey P, Green R, Xu W
BioMed research international2015968743(2015)

DEAD-box helicase DP103 defines metastatic potential of human breast cancers.
Shin EM, Hay HS, Lee MH, Goh JN, Tan TZ, Sen YP, Lim SW, Yousef EM, Ong HT, Thike AA, Kong X, Wu Z, Mendoz E, Sun W, Salto-Tellez M, Lim CT, Lobie PE, Lim YP, Yap CT, Zeng Q, Sethi G, Lee MB, Tan P, Goh BC, Miller LD, Thiery JP, Zhu T, Gaboury L, Tan PH, Hui KM, Yip GW, Miyamoto S, Kumar AP, Tergaonkar V
The Journal of clinical investigation124(9)3807-24(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The findings show that DDX20 may promote the progression of prostate cancer through the NF-kappaB pathway.
Title: High expression of DDX20 enhances the proliferation and metastatic potential of prostate cancer cells through the NF-?B pathway.

In stage-adjusted analysis, the nominal associations of DDX20-rs197412, PTGS2-rs5275, and HSPA5-rs391957 with DFS were detected. However, after FDR correction none of these polymorphisms remained significantly associated with the survival outcomes
Title: A Survival Association Study of 102 Polymorphisms Previously Associated with Survival Outcomes in Colorectal Cancer.

DP103 defines metastatic potential of human breast cancers.
Title: DEAD-box helicase DP103 defines metastatic potential of human breast cancers.

Impairment of miRNA-140 function due to a deficiency of DDX20, a miRNA machinery component, could lead to hepatocarcinogenesis
Title: MicroRNA-140 acts as a liver tumor suppressor by controlling NF-?B activity by directly targeting DNA methyltransferase 1 (Dnmt1) expression.

While DDX20 normally suppresses NF-kappaB activity by regulating NF-kappaB-suppressing miRNA-140 function, this suppressive effect was lost in DDX20-deficient cells.
Title: A miRNA machinery component DDX20 controls NF-?B via microRNA-140 function.

The following DDX20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11109	NM_007204.5 Latest version!	Homo sapiens DEAD-box helicase 20 (DDX20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
View Old Accession Versions >>
OHu11109	NM_007204.4	Homo sapiens DEAD-box helicase 20 (DDX20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX20 ( NM_007204.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_009135.4 Homo sapiens DEAD-box helicase 20 (DDX20), mRNA.

Price & Availability↑

CloneID	OHu11109
Clone ID Related Accession (Same CDS sequence)	NM_007204.5 , NM_007204.4
Accession Version	NM_007204.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF171063.1, AK001506.1, AY007146.1 and AL049557.19. On Nov 23, 2018 this sequence version replaced NM_007204.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.60465.1, SRR1660803.35670.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000369702.5/ ENSP00000358716.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG CATTTGAAGC CTCGGGAGCC TTAGCAGCAG TGGCGACTGC TATGCCGGCT 
GAGCATGTGG CCGTGCAGGT CCCGGCCCCA GAGCCAACAC CCGGGCCTGT GAGGATCCTG 
CGGACCGCTC AGGATCTCAG CAGCCCGCGG ACCCGCACGG GGGATGTGCT GTTGGCGGAG 
CCGGCCGACT TCGAGTCACT GCTGCTTTCG CGGCCGGTGC TGGAGGGGCT GCGGGCGGCC 
GGCTTCGAGA GGCCCTCGCC GGTGCAGCTC AAGGCCATCC CGTTGGGGCG CTGCGGGCTC 
GATTTAATTG TTCAAGCTAA ATCTGGCACC GGGAAAACCT GTGTGTTCTC CACCATAGCT 
TTGGACTCTC TTGTTCTTGA AAACTTAAGT ACCCAGATTT TGATCTTGGC TCCTACAAGA 
GAAATTGCTG TACAGATACA TTCTGTTATT ACAGCCATTG GAATAAAAAT GGAAGGCTTA 
GAGTGTCATG TCTTTATTGG AGGGACCCCA TTATCACAAG ACAAAACCAG ACTTAAAAAG 
TGTCATATTG CTGTTGGATC TCCTGGCAGA ATTAAGCAAC TCATAGAACT TGACTACTTG 
AACCCAGGCA GTATACGCCT CTTTATTCTT GATGAAGCAG ATAAGCTTTT AGAAGAAGGC 
AGCTTCCAGG AGCAAATAAA TTGGATTTAT TCTTCCTTGC CTGCCAGTAA ACAGATGCTG 
GCAGTATCAG CTACTTATCC CGAATTTTTG GCTAATGCTT TGACAAAGTA CATGAGAGAT 
CCCACTTTTG TAAGACTGAA TTCCAGTGAT CCAAGTCTCA TAGGTTTGAA GCAGTATTAC 
AAAGTTGTCA ATTCATACCC TTTGGCACAT AAGGTTTTTG AGGAAAAGAC TCAGCATTTA 
CAGGAACTGT TCAGCAGAAT TCCATTTAAT CAAGCTTTAG TCTTTTCTAA TTTGCACAGC 
AGAGCACAAC ATTTGGCTGA TATCCTTTCT TCTAAAGGCT TTCCTGCTGA GTGCATTTCA 
GGCAATATGA ATCAGAATCA GCGTCTTGAT GCTATGGCTA AACTGAAGCA CTTTCATTGC 
AGAGTCCTCA TTTCCACAGA TTTGACTTCT CGTGGGATTG ATGCTGAGAA GGTGAATCTG 
GTTGTAAATC TGGATGTACC ATTGGATTGG GAGACATACA TGCATCGGAT TGGGAGAGCT 
GGCCGTTTTG GTACATTGGG GCTGACAGTG ACCTACTGTT GCCGGGGAGA GGAAGAAAAT 
ATGATGATGA GAATTGCCCA GAAATGTAAT ATCAACCTTC TCCCTTTACC AGATCCCATT 
CCTTCTGGTC TGATGGAAGA ATGTGTGGAT TGGGATGTGG AAGTTAAAGC TGCTGTGCAT 
ACATATGGTA TAGCAAGTGT ACCTAACCAA CCCTTAAAAA AGCAAATTCA GAAAATAGAG 
AGAACCCTTC AAATTCAGAA AGCTCATGGT GACCACATGG CTTCCTCTAG AAATAATTCT 
GTATCTGGAC TATCAGTCAA ATCAAAAAAT AATACCAAAC AAAAGCTTCC TGTGAAAAGC 
CACTCAGAAT GTGGAATCAT AGAAAAAGCA ACGTCACCAA AAGAACTGGG CTGTGACAGG 
CAATCCGAAG AGCAAATGAA GAATTCTGTT CAGACTCCCG TTGAAAACTC CACCAACAGT 
CAGCACCAGG TCAAAGAAGC TTTACCTGTG TCACTCCCCC AGATTCCTTG TCTGTCTTCC 
TTTAAAATCC ATCAGCCATA CACGTTGACT TTTGCTGAAT TGGTAGAGGA TTATGAACAT 
TATATTAAAG AGGGGTTAGA GAAACCTGTG GAAATCATCA GGCACTACAC AGGCCCTGGG 
GATCAGACTG TGAATCCTCA AAATGGTTTT GTGAGAAATA AAGTTATTGA ACAGAGAGTC 
CCTGTGTTGG CAAGTAGTAG CCAATCTGGA GACTCTGAGA GTGACAGTGA TTCTTACAGC 
TCAAGAACCT CTTCCCAGAG CAAAGGAAAT AAGTCATACT TGGAAGGCTC TTCTGATAAT 
CAGCTGAAAG ACTCTGAATC TACGCCTGTG GATGATCGTA TTTCTTTGGA ACAACCACCA 
AATGGAAGTG ACACCCCCAA TCCAGAGAAA TATCAAGAAT CACCTGGAAT CCAGATGAAG 
ACAAGACTTA AAGAGGGGGC TAGCCAGAGA GCTAAGCAGA GCCGGAGAAA CCTACCCAGG 
CGGTCTTCCT TCAGATTGCA GACTGAAGCC CAGGAAGATG ATTGGTATGA CTGTCATAGG 
GAAATACGTC TGAGTTTTTC TGATACCTAT CAGGATTATG AGGAGTACTG GAGAGCTTAC 
TACAGGGCAT GGCAAGAATA TTATGCTGCC GCTTCTCATT CATATTATTG GAATGCTCAG 
AGACATCCAA GTTGGATGGC AGCTTATCAC ATGAATACCA TTTATCTACA AGAAATGATG 
CATAGTAACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009135.4
CDS	25..2499
Translation	MAAAFEASGALAAVATAMPAEHVAVQVPAPEPTPGPVRILRTAQDLSSPRTRTGDVLLAEPADFESLLLSRPVLEGLRAAGFERPSPVQLKAIPLGRCGLDLIVQAKSGTGKTCVFSTIALDSLVLENLSTQILILAPTREIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPGRIKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQMLAVSATYPEFLANALTKYMRDPTFVRLNSSDPSLIGLKQYYKVVNSYPLAHKVFEEKTQHLQELFSRIPFNQALVFSNLHSRAQHLADILSSKGFPAECISGNMNQNQRLDAMAKLKHFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLDWETYMHRIGRAGRFGTLGLTVTYCCRGEEENMMMRIAQKCNINLLPLPDPIPSGLMEECVDWDVEVKAAVHTYGIASVPNQPLKKQIQKIERTLQIQKAHGDHMASSRNNSVSGLSVKSKNNTKQKLPVKSHSECGIIEKATSPKELGCDRQSEEQMKNSVQTPVENSTNSQHQVKEALPVSLPQIPCLSSFKIHQPYTLTFAELVEDYEHYIKEGLEKPVEIIRHYTGPGDQTVNPQNGFVRNKVIEQRVPVLASSSQSGDSESDSDSYSSRTSSQSKGNKSYLEGSSDNQLKDSESTPVDDRISLEQPPNGSDTPNPEKYQESPGIQMKTRLKEGASQRAKQSRRNLPRRSSFRLQTEAQEDDWYDCHREIRLSFSDTYQDYEEYWRAYYRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTIYLQEMMHSNQ

Target ORF information:

RefSeq Version	NM_007204.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 20 (DDX20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007204.5

ORF Insert Sequence:

ATGGCGGCGG CATTTGAAGC CTCGGGAGCC TTAGCAGCAG TGGCGACTGC TATGCCGGCT 
GAGCATGTGG CCGTGCAGGT CCCGGCCCCA GAGCCAACAC CCGGGCCTGT GAGGATCCTG 
CGGACCGCTC AGGATCTCAG CAGCCCGCGG ACCCGCACGG GGGATGTGCT GTTGGCGGAG 
CCGGCCGACT TCGAGTCACT GCTGCTTTCG CGGCCGGTGC TGGAGGGGCT GCGGGCGGCC 
GGCTTCGAGA GGCCCTCGCC GGTGCAGCTC AAGGCCATCC CGTTGGGGCG CTGCGGGCTC 
GATTTAATTG TTCAAGCTAA ATCTGGCACC GGGAAAACCT GTGTGTTCTC CACCATAGCT 
TTGGACTCTC TTGTTCTTGA AAACTTAAGT ACCCAGATTT TGATCTTGGC TCCTACAAGA 
GAAATTGCTG TACAGATACA TTCTGTTATT ACAGCCATTG GAATAAAAAT GGAAGGCTTA 
GAGTGTCATG TCTTTATTGG AGGGACCCCA TTATCACAAG ACAAAACCAG ACTTAAAAAG 
TGTCATATTG CTGTTGGATC TCCTGGCAGA ATTAAGCAAC TCATAGAACT TGACTACTTG 
AACCCAGGCA GTATACGCCT CTTTATTCTT GATGAAGCAG ATAAGCTTTT AGAAGAAGGC 
AGCTTCCAGG AGCAAATAAA TTGGATTTAT TCTTCCTTGC CTGCCAGTAA ACAGATGCTG 
GCAGTATCAG CTACTTATCC CGAATTTTTG GCTAATGCTT TGACAAAGTA CATGAGAGAT 
CCCACTTTTG TAAGACTGAA TTCCAGTGAT CCAAGTCTCA TAGGTTTGAA GCAGTATTAC 
AAAGTTGTCA ATTCATACCC TTTGGCACAT AAGGTTTTTG AGGAAAAGAC TCAGCATTTA 
CAGGAACTGT TCAGCAGAAT TCCATTTAAT CAAGCTTTAG TCTTTTCTAA TTTGCACAGC 
AGAGCACAAC ATTTGGCTGA TATCCTTTCT TCTAAAGGCT TTCCTGCTGA GTGCATTTCA 
GGCAATATGA ATCAGAATCA GCGTCTTGAT GCTATGGCTA AACTGAAGCA CTTTCATTGC 
AGAGTCCTCA TTTCCACAGA TTTGACTTCT CGTGGGATTG ATGCTGAGAA GGTGAATCTG 
GTTGTAAATC TGGATGTACC ATTGGATTGG GAGACATACA TGCATCGGAT TGGGAGAGCT 
GGCCGTTTTG GTACATTGGG GCTGACAGTG ACCTACTGTT GCCGGGGAGA GGAAGAAAAT 
ATGATGATGA GAATTGCCCA GAAATGTAAT ATCAACCTTC TCCCTTTACC AGATCCCATT 
CCTTCTGGTC TGATGGAAGA ATGTGTGGAT TGGGATGTGG AAGTTAAAGC TGCTGTGCAT 
ACATATGGTA TAGCAAGTGT ACCTAACCAA CCCTTAAAAA AGCAAATTCA GAAAATAGAG 
AGAACCCTTC AAATTCAGAA AGCTCATGGT GACCACATGG CTTCCTCTAG AAATAATTCT 
GTATCTGGAC TATCAGTCAA ATCAAAAAAT AATACCAAAC AAAAGCTTCC TGTGAAAAGC 
CACTCAGAAT GTGGAATCAT AGAAAAAGCA ACGTCACCAA AAGAACTGGG CTGTGACAGG 
CAATCCGAAG AGCAAATGAA GAATTCTGTT CAGACTCCCG TTGAAAACTC CACCAACAGT 
CAGCACCAGG TCAAAGAAGC TTTACCTGTG TCACTCCCCC AGATTCCTTG TCTGTCTTCC 
TTTAAAATCC ATCAGCCATA CACGTTGACT TTTGCTGAAT TGGTAGAGGA TTATGAACAT 
TATATTAAAG AGGGGTTAGA GAAACCTGTG GAAATCATCA GGCACTACAC AGGCCCTGGG 
GATCAGACTG TGAATCCTCA AAATGGTTTT GTGAGAAATA AAGTTATTGA ACAGAGAGTC 
CCTGTGTTGG CAAGTAGTAG CCAATCTGGA GACTCTGAGA GTGACAGTGA TTCTTACAGC 
TCAAGAACCT CTTCCCAGAG CAAAGGAAAT AAGTCATACT TGGAAGGCTC TTCTGATAAT 
CAGCTGAAAG ACTCTGAATC TACGCCTGTG GATGATCGTA TTTCTTTGGA ACAACCACCA 
AATGGAAGTG ACACCCCCAA TCCAGAGAAA TATCAAGAAT CACCTGGAAT CCAGATGAAG 
ACAAGACTTA AAGAGGGGGC TAGCCAGAGA GCTAAGCAGA GCCGGAGAAA CCTACCCAGG 
CGGTCTTCCT TCAGATTGCA GACTGAAGCC CAGGAAGATG ATTGGTATGA CTGTCATAGG 
GAAATACGTC TGAGTTTTTC TGATACCTAT CAGGATTATG AGGAGTACTG GAGAGCTTAC 
TACAGGGCAT GGCAAGAATA TTATGCTGCC GCTTCTCATT CATATTATTG GAATGCTCAG 
AGACATCCAA GTTGGATGGC AGCTTATCAC ATGAATACCA TTTATCTACA AGAAATGATG 
CATAGTAACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX20 ( NM_007204.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_009135.4 Homo sapiens DEAD-box helicase 20 (DDX20), mRNA.

Price & Availability↑

CloneID	OHu11109
Clone ID Related Accession (Same CDS sequence)	NM_007204.5 , NM_007204.4
Accession Version	NM_007204.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB072947.1, AF171063.1, AK001506.1, AY007146.1 and AL049557.19. On Aug 20, 2009 this sequence version replaced NM_007204.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034953.2, SRR1803617.60465.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CATTTGAAGC CTCGGGAGCC TTAGCAGCAG TGGCGACTGC TATGCCGGCT 
GAGCATGTGG CCGTGCAGGT CCCGGCCCCA GAGCCAACAC CCGGGCCTGT GAGGATCCTG 
CGGACCGCTC AGGATCTCAG CAGCCCGCGG ACCCGCACGG GGGATGTGCT GTTGGCGGAG 
CCGGCCGACT TCGAGTCACT GCTGCTTTCG CGGCCGGTGC TGGAGGGGCT GCGGGCGGCC 
GGCTTCGAGA GGCCCTCGCC GGTGCAGCTC AAGGCCATCC CGTTGGGGCG CTGCGGGCTC 
GATTTAATTG TTCAAGCTAA ATCTGGCACC GGGAAAACCT GTGTGTTCTC CACCATAGCT 
TTGGACTCTC TTGTTCTTGA AAACTTAAGT ACCCAGATTT TGATCTTGGC TCCTACAAGA 
GAAATTGCTG TACAGATACA TTCTGTTATT ACAGCCATTG GAATAAAAAT GGAAGGCTTA 
GAGTGTCATG TCTTTATTGG AGGGACCCCA TTATCACAAG ACAAAACCAG ACTTAAAAAG 
TGTCATATTG CTGTTGGATC TCCTGGCAGA ATTAAGCAAC TCATAGAACT TGACTACTTG 
AACCCAGGCA GTATACGCCT CTTTATTCTT GATGAAGCAG ATAAGCTTTT AGAAGAAGGC 
AGCTTCCAGG AGCAAATAAA TTGGATTTAT TCTTCCTTGC CTGCCAGTAA ACAGATGCTG 
GCAGTATCAG CTACTTATCC CGAATTTTTG GCTAATGCTT TGACAAAGTA CATGAGAGAT 
CCCACTTTTG TAAGACTGAA TTCCAGTGAT CCAAGTCTCA TAGGTTTGAA GCAGTATTAC 
AAAGTTGTCA ATTCATACCC TTTGGCACAT AAGGTTTTTG AGGAAAAGAC TCAGCATTTA 
CAGGAACTGT TCAGCAGAAT TCCATTTAAT CAAGCTTTAG TCTTTTCTAA TTTGCACAGC 
AGAGCACAAC ATTTGGCTGA TATCCTTTCT TCTAAAGGCT TTCCTGCTGA GTGCATTTCA 
GGCAATATGA ATCAGAATCA GCGTCTTGAT GCTATGGCTA AACTGAAGCA CTTTCATTGC 
AGAGTCCTCA TTTCCACAGA TTTGACTTCT CGTGGGATTG ATGCTGAGAA GGTGAATCTG 
GTTGTAAATC TGGATGTACC ATTGGATTGG GAGACATACA TGCATCGGAT TGGGAGAGCT 
GGCCGTTTTG GTACATTGGG GCTGACAGTG ACCTACTGTT GCCGGGGAGA GGAAGAAAAT 
ATGATGATGA GAATTGCCCA GAAATGTAAT ATCAACCTTC TCCCTTTACC AGATCCCATT 
CCTTCTGGTC TGATGGAAGA ATGTGTGGAT TGGGATGTGG AAGTTAAAGC TGCTGTGCAT 
ACATATGGTA TAGCAAGTGT ACCTAACCAA CCCTTAAAAA AGCAAATTCA GAAAATAGAG 
AGAACCCTTC AAATTCAGAA AGCTCATGGT GACCACATGG CTTCCTCTAG AAATAATTCT 
GTATCTGGAC TATCAGTCAA ATCAAAAAAT AATACCAAAC AAAAGCTTCC TGTGAAAAGC 
CACTCAGAAT GTGGAATCAT AGAAAAAGCA ACGTCACCAA AAGAACTGGG CTGTGACAGG 
CAATCCGAAG AGCAAATGAA GAATTCTGTT CAGACTCCCG TTGAAAACTC CACCAACAGT 
CAGCACCAGG TCAAAGAAGC TTTACCTGTG TCACTCCCCC AGATTCCTTG TCTGTCTTCC 
TTTAAAATCC ATCAGCCATA CACGTTGACT TTTGCTGAAT TGGTAGAGGA TTATGAACAT 
TATATTAAAG AGGGGTTAGA GAAACCTGTG GAAATCATCA GGCACTACAC AGGCCCTGGG 
GATCAGACTG TGAATCCTCA AAATGGTTTT GTGAGAAATA AAGTTATTGA ACAGAGAGTC 
CCTGTGTTGG CAAGTAGTAG CCAATCTGGA GACTCTGAGA GTGACAGTGA TTCTTACAGC 
TCAAGAACCT CTTCCCAGAG CAAAGGAAAT AAGTCATACT TGGAAGGCTC TTCTGATAAT 
CAGCTGAAAG ACTCTGAATC TACGCCTGTG GATGATCGTA TTTCTTTGGA ACAACCACCA 
AATGGAAGTG ACACCCCCAA TCCAGAGAAA TATCAAGAAT CACCTGGAAT CCAGATGAAG 
ACAAGACTTA AAGAGGGGGC TAGCCAGAGA GCTAAGCAGA GCCGGAGAAA CCTACCCAGG 
CGGTCTTCCT TCAGATTGCA GACTGAAGCC CAGGAAGATG ATTGGTATGA CTGTCATAGG 
GAAATACGTC TGAGTTTTTC TGATACCTAT CAGGATTATG AGGAGTACTG GAGAGCTTAC 
TACAGGGCAT GGCAAGAATA TTATGCTGCC GCTTCTCATT CATATTATTG GAATGCTCAG 
AGACATCCAA GTTGGATGGC AGCTTATCAC ATGAATACCA TTTATCTACA AGAAATGATG 
CATAGTAACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009135.4
CDS	358..2832
Translation	MAAAFEASGALAAVATAMPAEHVAVQVPAPEPTPGPVRILRTAQDLSSPRTRTGDVLLAEPADFESLLLSRPVLEGLRAAGFERPSPVQLKAIPLGRCGLDLIVQAKSGTGKTCVFSTIALDSLVLENLSTQILILAPTREIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPGRIKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQMLAVSATYPEFLANALTKYMRDPTFVRLNSSDPSLIGLKQYYKVVNSYPLAHKVFEEKTQHLQELFSRIPFNQALVFSNLHSRAQHLADILSSKGFPAECISGNMNQNQRLDAMAKLKHFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLDWETYMHRIGRAGRFGTLGLTVTYCCRGEEENMMMRIAQKCNINLLPLPDPIPSGLMEECVDWDVEVKAAVHTYGIASVPNQPLKKQIQKIERTLQIQKAHGDHMASSRNNSVSGLSVKSKNNTKQKLPVKSHSECGIIEKATSPKELGCDRQSEEQMKNSVQTPVENSTNSQHQVKEALPVSLPQIPCLSSFKIHQPYTLTFAELVEDYEHYIKEGLEKPVEIIRHYTGPGDQTVNPQNGFVRNKVIEQRVPVLASSSQSGDSESDSDSYSSRTSSQSKGNKSYLEGSSDNQLKDSESTPVDDRISLEQPPNGSDTPNPEKYQESPGIQMKTRLKEGASQRAKQSRRNLPRRSSFRLQTEAQEDDWYDCHREIRLSFSDTYQDYEEYWRAYYRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTIYLQEMMHSNQ

Target ORF information:

RefSeq Version	NM_007204.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 20 (DDX20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007204.4

ORF Insert Sequence:

ATGGCGGCGG CATTTGAAGC CTCGGGAGCC TTAGCAGCAG TGGCGACTGC TATGCCGGCT 
GAGCATGTGG CCGTGCAGGT CCCGGCCCCA GAGCCAACAC CCGGGCCTGT GAGGATCCTG 
CGGACCGCTC AGGATCTCAG CAGCCCGCGG ACCCGCACGG GGGATGTGCT GTTGGCGGAG 
CCGGCCGACT TCGAGTCACT GCTGCTTTCG CGGCCGGTGC TGGAGGGGCT GCGGGCGGCC 
GGCTTCGAGA GGCCCTCGCC GGTGCAGCTC AAGGCCATCC CGTTGGGGCG CTGCGGGCTC 
GATTTAATTG TTCAAGCTAA ATCTGGCACC GGGAAAACCT GTGTGTTCTC CACCATAGCT 
TTGGACTCTC TTGTTCTTGA AAACTTAAGT ACCCAGATTT TGATCTTGGC TCCTACAAGA 
GAAATTGCTG TACAGATACA TTCTGTTATT ACAGCCATTG GAATAAAAAT GGAAGGCTTA 
GAGTGTCATG TCTTTATTGG AGGGACCCCA TTATCACAAG ACAAAACCAG ACTTAAAAAG 
TGTCATATTG CTGTTGGATC TCCTGGCAGA ATTAAGCAAC TCATAGAACT TGACTACTTG 
AACCCAGGCA GTATACGCCT CTTTATTCTT GATGAAGCAG ATAAGCTTTT AGAAGAAGGC 
AGCTTCCAGG AGCAAATAAA TTGGATTTAT TCTTCCTTGC CTGCCAGTAA ACAGATGCTG 
GCAGTATCAG CTACTTATCC CGAATTTTTG GCTAATGCTT TGACAAAGTA CATGAGAGAT 
CCCACTTTTG TAAGACTGAA TTCCAGTGAT CCAAGTCTCA TAGGTTTGAA GCAGTATTAC 
AAAGTTGTCA ATTCATACCC TTTGGCACAT AAGGTTTTTG AGGAAAAGAC TCAGCATTTA 
CAGGAACTGT TCAGCAGAAT TCCATTTAAT CAAGCTTTAG TCTTTTCTAA TTTGCACAGC 
AGAGCACAAC ATTTGGCTGA TATCCTTTCT TCTAAAGGCT TTCCTGCTGA GTGCATTTCA 
GGCAATATGA ATCAGAATCA GCGTCTTGAT GCTATGGCTA AACTGAAGCA CTTTCATTGC 
AGAGTCCTCA TTTCCACAGA TTTGACTTCT CGTGGGATTG ATGCTGAGAA GGTGAATCTG 
GTTGTAAATC TGGATGTACC ATTGGATTGG GAGACATACA TGCATCGGAT TGGGAGAGCT 
GGCCGTTTTG GTACATTGGG GCTGACAGTG ACCTACTGTT GCCGGGGAGA GGAAGAAAAT 
ATGATGATGA GAATTGCCCA GAAATGTAAT ATCAACCTTC TCCCTTTACC AGATCCCATT 
CCTTCTGGTC TGATGGAAGA ATGTGTGGAT TGGGATGTGG AAGTTAAAGC TGCTGTGCAT 
ACATATGGTA TAGCAAGTGT ACCTAACCAA CCCTTAAAAA AGCAAATTCA GAAAATAGAG 
AGAACCCTTC AAATTCAGAA AGCTCATGGT GACCACATGG CTTCCTCTAG AAATAATTCT 
GTATCTGGAC TATCAGTCAA ATCAAAAAAT AATACCAAAC AAAAGCTTCC TGTGAAAAGC 
CACTCAGAAT GTGGAATCAT AGAAAAAGCA ACGTCACCAA AAGAACTGGG CTGTGACAGG 
CAATCCGAAG AGCAAATGAA GAATTCTGTT CAGACTCCCG TTGAAAACTC CACCAACAGT 
CAGCACCAGG TCAAAGAAGC TTTACCTGTG TCACTCCCCC AGATTCCTTG TCTGTCTTCC 
TTTAAAATCC ATCAGCCATA CACGTTGACT TTTGCTGAAT TGGTAGAGGA TTATGAACAT 
TATATTAAAG AGGGGTTAGA GAAACCTGTG GAAATCATCA GGCACTACAC AGGCCCTGGG 
GATCAGACTG TGAATCCTCA AAATGGTTTT GTGAGAAATA AAGTTATTGA ACAGAGAGTC 
CCTGTGTTGG CAAGTAGTAG CCAATCTGGA GACTCTGAGA GTGACAGTGA TTCTTACAGC 
TCAAGAACCT CTTCCCAGAG CAAAGGAAAT AAGTCATACT TGGAAGGCTC TTCTGATAAT 
CAGCTGAAAG ACTCTGAATC TACGCCTGTG GATGATCGTA TTTCTTTGGA ACAACCACCA 
AATGGAAGTG ACACCCCCAA TCCAGAGAAA TATCAAGAAT CACCTGGAAT CCAGATGAAG 
ACAAGACTTA AAGAGGGGGC TAGCCAGAGA GCTAAGCAGA GCCGGAGAAA CCTACCCAGG 
CGGTCTTCCT TCAGATTGCA GACTGAAGCC CAGGAAGATG ATTGGTATGA CTGTCATAGG 
GAAATACGTC TGAGTTTTTC TGATACCTAT CAGGATTATG AGGAGTACTG GAGAGCTTAC 
TACAGGGCAT GGCAAGAATA TTATGCTGCC GCTTCTCATT CATATTATTG GAATGCTCAG 
AGACATCCAA GTTGGATGGC AGCTTATCAC ATGAATACCA TTTATCTACA AGAAATGATG 
CATAGTAACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of the polymorphic variants of RAN and GEMIN3 genes and risk of Primary Open-Angle Glaucoma in the Polish population.
Ophthalmic genetics1-9(2017 Nov)
Molasy M,Walczak A,Przyby?owska-Sygut K,Zaleska-?mijewska A,Szaflik J,Szaflik JP,Majsterek I

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

High expression of DDX20 enhances the proliferation and metastatic potential of prostate cancer cells through the NF-?B pathway.
International journal of molecular medicine37(6)1551-7(2016 Jun)
Chen W,Zhou P,Li X

A Survival Association Study of 102 Polymorphisms Previously Associated with Survival Outcomes in Colorectal Cancer.
BioMed research international2015968743(2015)
Savas S,Xu J,Werdyani S,Shestopaloff K,Dicks E,Green J,Parfrey P,Green R,Xu W

DEAD-box helicase DP103 defines metastatic potential of human breast cancers.
The Journal of clinical investigation124(9)3807-24(2014 Sep)
Shin EM,Hay HS,Lee MH,Goh JN,Tan TZ,Sen YP,Lim SW,Yousef EM,Ong HT,Thike AA,Kong X,Wu Z,Mendoz E,Sun W,Salto-Tellez M,Lim CT,Lobie PE,Lim YP,Yap CT,Zeng Q,Sethi G,Lee MB,Tan P,Goh BC,Miller LD,Thiery JP,Zhu T,Gaboury L,Tan PH,Hui KM,Yip GW,Miyamoto S,Kumar AP,Tergaonkar V

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DDX20 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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