ddx20 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx20
Entrez Gene ID	556273
Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Synonyms	si:dkey-193l3.3,si:dkey-193l3.4,wu:fb16g08,wu:fb59a11
General protein information	Preferred Names DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 Names probable ATP-dependent RNA helicase DDX20 DEAD box protein 20 fb59a11
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009303993.2	XP_009302268.1	probable ATP-dependent RNA helicase DDX20 isoform X1
NM_001123323.1	NP_001116795.1	probable ATP-dependent RNA helicase DDX20
XM_009303993.3	XP_009302268.1	probable ATP-dependent RNA helicase DDX20 isoform X1
XM_021478262.1	XP_021333937.1	probable ATP-dependent RNA helicase DDX20 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DDX20	NP_001095588.1
Pan troglodytes (chimpanzee)	DDX20	XP_003308378.1
Danio rerio (zebrafish)	ddx20	NP_001116795.1
Rattus norvegicus (Norway rat)	Ddx20	NP_001178640.1
Canis lupus familiaris (dog)	DDX20	XP_003639658.1
Mus musculus (house mouse)	Ddx20	NP_059093.3
Gallus gallus (chicken)	DDX20	XP_417996.1
Homo sapiens (human)	DDX20	NP_009135.4
Xenopus tropicalis (tropical clawed frog)	ddx20	XP_002932842.1

Gene Ontology
Bibliography

Related articles in PubMed

Forward Genetic Screens in Zebrafish Identify Pre-mRNA-Processing Pathways Regulating Early T Cell Development.
Iwanami N, Sikora K, Richter AS, M?nnich M, Guerri L, Soza-Ried C, Lawir DF, Mateos F, Hess I, O'Meara CP, Schorpp M, Boehm T
Cell reports17(9)2259-2270(2016 11)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Processing of fish Ig heavy chain transcripts: diverse splicing patterns and unusual nonsense mediated decay.
Quiniou SM, Wilson M, Boudinot P
Developmental and comparative immunology35(9)949-58(2011 Sep)

Global repression of cancer gene expression in a zebrafish model of melanoma is linked to epigenetic regulation.
Anelli V, Santoriello C, Distel M, K?ster RW, Ciccarelli FD, Mione M
Zebrafish6(4)417-24(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ddx20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00201	NM_001123323.1 Latest version!	Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa42332	XM_009303993.3 Latest version!	Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa14223	XM_021478262.1 Latest version!	Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa42332	XM_009303993.2	Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx20 ( NM_001123323.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116795.1 Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), mRNA.

Price & Availability↑

CloneID	ODa00201
Clone ID Related Accession (Same CDS sequence)	NM_001123323.1
Accession Version	NM_001123323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	probable ATP-dependent RNA helicase DDX20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX664742.15. On Apr 11, 2008 this sequence version replaced XM_001342308.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGTGCGC AGGAGGGCGC AGTGATTCAC AGCTCATGTC ATTTATTCAC CGTAGAAGAA 
ACAGAACGTT GTCGCGATAG AAACATGGCG GCATCTGTGA AAGCAGCGCA TGAGCTGCAA 
TCGAGAACCC GGACGGATGA TGTTTTGATT AGCGGCGGTG TCGAGTTCAG CTCTTTACTC 
CTCTCAAAAC CCGTGCTTGA GGGTTTATCT GCTTCCGGTT TTCAGAGACC CTCTCCCATC 
CAGCTAAAGG CAATCCCGCT GGGAAGATGT GGACTCGATC TAATTGTTCA AGCCAAATCT 
GGCACAGGAA AGACCTGTGT GTTCACCACC ATCGCCTTGG ACTCTCTTAT ATTGGAAAAC 
GCAACTACAC AGGTGCTGGT TTTGGCCCCC ACTCGGGAGA TTGCAGTGCA GATTCACGCT 
GTTGTCATGG CGATAGGCAG TGCTATGGAG GGTTTGGAGT GCCATGTGTT CATTGGTGGT 
CGACCTATCA GTCAAGATAA ACAGCACCTG AAGAAGTGCC ACATTGCTAT TGGGTCACCA 
GGTCGTATTA AGCAGCTAAT TGAGATGGGA GCCCTGATGG TGTCCTCCAT TCGCTTGTTT 
GTTTTGGATG AGGCTGATAA ACTCCTGGAG GACGACAGCA GCAGCAGCTT TCAGGAGCAG 
ATCAACTGGA TCTACTCCTC TCTACCAGCA AATAAACAAA TGTTGGCTCT TTCTGCCACC 
TACCCAGAGT CTTTAGCACA ACAGTTGTCA AGATACATGA GAGAGCCCAC GTTTGTGCGA 
CTGAACCCAA CTGACCCTGG ACTCCTCGGA CTGAAGCAGT ATTATAAGAT TGTGCCATCT 
CATTCATTGC CTCATAAAGT TTTTGAGGAG AAGGTGCAGA GTCTTCTTGA ACTCTTCAGT 
AAAATCCCAT TTAACCAAGC GCTGGTGTTC TCGAATCTTC ATACAGCTCA GCATCTGGCA 
GATATCCTCT CCTCTAAAGG TTTGCCTGCA GTCTGCATCT CAGGTGGTTT AAGTCAGGAT 
CAGAGGTTAG AGGCGATGTG GAAGCTGAAG CAGTATCAGT GTCGAGTTTT AATATCCACT 
GATTTGACGT CACGTGGAAT TGATGCTGAG AAGGTGAACT TGGTGATTAA TCTGGACGTT 
CCTCAGGACT GGGAGACCTA CATGCACCGT ATTGGTCGTG CTGGACGTTT TGGTACTCTG 
GGGGTTGCCG TGACCTACTG TTGCCATGGG GAGGAGGAGA ACAAGATGAT GGCCATCGCT 
CAGAAGTGCT CTCTGGATCT CATGCATTTA CCAGATCCAA TACCTCCAGG CATCATGGAA 
GAAGCGTGTG ACCATGATGT GATCATAGAA ACTCTTTCAA AACCAAAGAT TCCTGACAAT 
TTCAAACCAG AGAAGAAGAA AAAGGCTAAA TCAGAACAGA TGAAGTCTAA ACCCTCTAGA 
GAGTCTCACA TCTCTGTGGT CATCACAGAA AGCGTGACCG GTGACAAATC ATGCAGTCGA 
GCTGAAACCA AGACTAAAAA TCATTCTTCC AAACCTGGGA GGCAAAAACA AATGGCGCCT 
GCTGCAAACC TCCAACATGA CTCGACTATT ACTCAGAAAC AGCAGAATGA CACCCTTCCC 
AAAATTCCTC CTTTGTCATC CTTTAAGTCG CACACAGGGA AGAGAATAAC CTTCGACGAT 
GCTTTGGTGG ATTATGAGAC CTTTATAAGA GAAGGTCCGG GAAGATCTGT TGAAATCATT 
CACCAGTATG ATGGTTTCTA CAGGAGTCAA GATAAAGTTA ATGGTCTTCA TGACGCCCAT 
GATGAAGATG AGGCTTCTGA ATCATACGTT CTAGAAGAAT CACAGTCCCA TCAAGATCTT 
CCACCAGCGC AGGAGGTTTC AGAGTTGTCC TCAGAGCAGA AGACATCTAG TGAATCCTCT 
TCTGAGTCTG AGATGGAAGT GGAGAGTGAA TCAAGCTCTT CTGTGCCTAG CAAGTCTACA 
CTTGAAGCAC TTCCAGATAA TAAAGTTTCG CTTGTACGTA ATCATTCAGA ATTAGCTGAA 
TTAGCTTTAC CTTCAACCAG AGTACCACAA CAGGCCACCA GAAGCAAGAA AAGGCCATGT 
CAGCCTCAAT CCCAGGACAC CGCTCACCAT CACAATCTTC CTCATAAACA CCGAACAGCC 
TCCAAATCTA GCCGGAGGCC AACAGGACCA AAGCGGAGAA CAAGAGAGAC TGATGAGACT 
GAGGAGGAAG AGGCTGGACA GGATTATTGG AGCTCGTACA GGGCCTGGGC TGAATATTAC 
AATTCATATT ATCACCATTC GCCCTATAAT TGGATGACTG CGTTTTATAT GAATTCTGTG 
TACATAAAGG AAATGATGAA GCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116795.1
CDS	398..2764
Translation	MCAQEGAVIHSSCHLFTVEETERCRDRNMAASVKAAHELQSRTRTDDVLISGGVEFSSLLLSKPVLEGLSASGFQRPSPIQLKAIPLGRCGLDLIVQAKSGTGKTCVFTTIALDSLILENATTQVLVLAPTREIAVQIHAVVMAIGSAMEGLECHVFIGGRPISQDKQHLKKCHIAIGSPGRIKQLIEMGALMVSSIRLFVLDEADKLLEDDSSSSFQEQINWIYSSLPANKQMLALSATYPESLAQQLSRYMREPTFVRLNPTDPGLLGLKQYYKIVPSHSLPHKVFEEKVQSLLELFSKIPFNQALVFSNLHTAQHLADILSSKGLPAVCISGGLSQDQRLEAMWKLKQYQCRVLISTDLTSRGIDAEKVNLVINLDVPQDWETYMHRIGRAGRFGTLGVAVTYCCHGEEENKMMAIAQKCSLDLMHLPDPIPPGIMEEACDHDVIIETLSKPKIPDNFKPEKKKKAKSEQMKSKPSRESHISVVITESVTGDKSCSRAETKTKNHSSKPGRQKQMAPAANLQHDSTITQKQQNDTLPKIPPLSSFKSHTGKRITFDDALVDYETFIREGPGRSVEIIHQYDGFYRSQDKVNGLHDAHDEDEASESYVLEESQSHQDLPPAQEVSELSSEQKTSSESSSESEMEVESESSSSVPSKSTLEALPDNKVSLVRNHSELAELALPSTRVPQQATRSKKRPCQPQSQDTAHHHNLPHKHRTASKSSRRPTGPKRRTRETDETEEEEAGQDYWSSYRAWAEYYNSYYHHSPYNWMTAFYMNSVYIKEMMKH

Target ORF information:

RefSeq Version	NM_001123323.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123323.1

ORF Insert Sequence:

ATGTGTGCGC AGGAGGGCGC AGTGATTCAC AGCTCATGTC ATTTATTCAC CGTAGAAGAA 
ACAGAACGTT GTCGCGATAG AAACATGGCG GCATCTGTGA AAGCAGCGCA TGAGCTGCAA 
TCGAGAACCC GGACGGATGA TGTTTTGATT AGCGGCGGTG TCGAGTTCAG CTCTTTACTC 
CTCTCAAAAC CCGTGCTTGA GGGTTTATCT GCTTCCGGTT TTCAGAGACC CTCTCCCATC 
CAGCTAAAGG CAATCCCGCT GGGAAGATGT GGACTCGATC TAATTGTTCA AGCCAAATCT 
GGCACAGGAA AGACCTGTGT GTTCACCACC ATCGCCTTGG ACTCTCTTAT ATTGGAAAAC 
GCAACTACAC AGGTGCTGGT TTTGGCCCCC ACTCGGGAGA TTGCAGTGCA GATTCACGCT 
GTTGTCATGG CGATAGGCAG TGCTATGGAG GGTTTGGAGT GCCATGTGTT CATTGGTGGT 
CGACCTATCA GTCAAGATAA ACAGCACCTG AAGAAGTGCC ACATTGCTAT TGGGTCACCA 
GGTCGTATTA AGCAGCTAAT TGAGATGGGA GCCCTGATGG TGTCCTCCAT TCGCTTGTTT 
GTTTTGGATG AGGCTGATAA ACTCCTGGAG GACGACAGCA GCAGCAGCTT TCAGGAGCAG 
ATCAACTGGA TCTACTCCTC TCTACCAGCA AATAAACAAA TGTTGGCTCT TTCTGCCACC 
TACCCAGAGT CTTTAGCACA ACAGTTGTCA AGATACATGA GAGAGCCCAC GTTTGTGCGA 
CTGAACCCAA CTGACCCTGG ACTCCTCGGA CTGAAGCAGT ATTATAAGAT TGTGCCATCT 
CATTCATTGC CTCATAAAGT TTTTGAGGAG AAGGTGCAGA GTCTTCTTGA ACTCTTCAGT 
AAAATCCCAT TTAACCAAGC GCTGGTGTTC TCGAATCTTC ATACAGCTCA GCATCTGGCA 
GATATCCTCT CCTCTAAAGG TTTGCCTGCA GTCTGCATCT CAGGTGGTTT AAGTCAGGAT 
CAGAGGTTAG AGGCGATGTG GAAGCTGAAG CAGTATCAGT GTCGAGTTTT AATATCCACT 
GATTTGACGT CACGTGGAAT TGATGCTGAG AAGGTGAACT TGGTGATTAA TCTGGACGTT 
CCTCAGGACT GGGAGACCTA CATGCACCGT ATTGGTCGTG CTGGACGTTT TGGTACTCTG 
GGGGTTGCCG TGACCTACTG TTGCCATGGG GAGGAGGAGA ACAAGATGAT GGCCATCGCT 
CAGAAGTGCT CTCTGGATCT CATGCATTTA CCAGATCCAA TACCTCCAGG CATCATGGAA 
GAAGCGTGTG ACCATGATGT GATCATAGAA ACTCTTTCAA AACCAAAGAT TCCTGACAAT 
TTCAAACCAG AGAAGAAGAA AAAGGCTAAA TCAGAACAGA TGAAGTCTAA ACCCTCTAGA 
GAGTCTCACA TCTCTGTGGT CATCACAGAA AGCGTGACCG GTGACAAATC ATGCAGTCGA 
GCTGAAACCA AGACTAAAAA TCATTCTTCC AAACCTGGGA GGCAAAAACA AATGGCGCCT 
GCTGCAAACC TCCAACATGA CTCGACTATT ACTCAGAAAC AGCAGAATGA CACCCTTCCC 
AAAATTCCTC CTTTGTCATC CTTTAAGTCG CACACAGGGA AGAGAATAAC CTTCGACGAT 
GCTTTGGTGG ATTATGAGAC CTTTATAAGA GAAGGTCCGG GAAGATCTGT TGAAATCATT 
CACCAGTATG ATGGTTTCTA CAGGAGTCAA GATAAAGTTA ATGGTCTTCA TGACGCCCAT 
GATGAAGATG AGGCTTCTGA ATCATACGTT CTAGAAGAAT CACAGTCCCA TCAAGATCTT 
CCACCAGCGC AGGAGGTTTC AGAGTTGTCC TCAGAGCAGA AGACATCTAG TGAATCCTCT 
TCTGAGTCTG AGATGGAAGT GGAGAGTGAA TCAAGCTCTT CTGTGCCTAG CAAGTCTACA 
CTTGAAGCAC TTCCAGATAA TAAAGTTTCG CTTGTACGTA ATCATTCAGA ATTAGCTGAA 
TTAGCTTTAC CTTCAACCAG AGTACCACAA CAGGCCACCA GAAGCAAGAA AAGGCCATGT 
CAGCCTCAAT CCCAGGACAC CGCTCACCAT CACAATCTTC CTCATAAACA CCGAACAGCC 
TCCAAATCTA GCCGGAGGCC AACAGGACCA AAGCGGAGAA CAAGAGAGAC TGATGAGACT 
GAGGAGGAAG AGGCTGGACA GGATTATTGG AGCTCGTACA GGGCCTGGGC TGAATATTAC 
AATTCATATT ATCACCATTC GCCCTATAAT TGGATGACTG CGTTTTATAT GAATTCTGTG 
TACATAAAGG AAATGATGAA GCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx20 ( XM_009303993.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302268.1 Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42332
Clone ID Related Accession (Same CDS sequence)	XM_009303993.2 , XM_009303993.3
Accession Version	XM_009303993.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable ATP-dependent RNA helicase DDX20 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAATCA TGAGTGTTTG TCGCCCTCTG CAGGACGTGG CTCGATATTC CAGGCAGCGG 
TTCTGTATGT GTGCGCAGGA GGGCGCAGTG ATTCACAGCT CATGTCATTT ATTCACCGTA 
GAAGAAACAG AACGTTGTCG CGATAGAAAC ATGGCGGCAT CTGTGAAAGC AGCGCATGAG 
CTGCAATCGA GAACCCGGAC GGATGATGTT TTGATTAGCG GCGGTGTCGA GTTCAGCTCT 
TTACTCCTCT CAAAACCCGT GCTTGAGGGT TTATCTGCTT CCGGTTTTCA GAGACCCTCT 
CCCATCCAGC TAAAGGCAAT CCCGCTGGGA AGATGTGGAC TCGATCTAAT TGTTCAAGCC 
AAATCTGGCA CAGGAAAGAC CTGTGTGTTC ACCACCATCG CCTTGGACTC TCTTATATTG 
GAAAACGCAA CTACACAGGT GCTGGTTTTG GCCCCCACTC GGGAGATTGC AGTGCAGATT 
CACGCTGTTG TCATGGCGAT AGGCAGTGCT ATGGAGGGTT TGGAGTGCCA TGTGTTCATT 
GGTGGTCGAC CTATCAGTCA AGATAAACAG CACCTGAAGA AGTGCCACAT TGCTATTGGG 
TCACCAGGTC GTATTAAGCA GCTAATTGAG ATGGGAGCCC TGATGGTGTC CTCCATTCGC 
TTGTTTGTTT TGGATGAGGC TGATAAACTC CTGGAGGACG ACAGCAGCAG CAGCTTTCAG 
GAGCAGATCA ACTGGATCTA CTCCTCTCTA CCAGCAAATA AACAAATGTT GGCTCTTTCT 
GCCACCTACC CAGAGTCTTT AGCACAACAG TTGTCAAGAT ACATGAGAGA GCCCACGTTT 
GTGCGACTGA ACCCAACTGA CCCTGGACTC CTCGGACTGA AGCAGTATTA TAAGATTGTG 
CCATCTCATT CATTGCCTCA TAAAGTTTTT GAGGAGAAGG TGCAGAGTCT TCTTGAACTC 
TTCAGTAAAA TCCCATTTAA CCAAGCGCTG GTGTTCTCGA ATCTTCATAC CAGAGCTCAG 
CATCTGGCAG ATATCCTCTC CTCTAAAGGT TTGCCTGCAG TCTGCATCTC AGGTGGTTTA 
AGTCAGGATC AGAGGTTAGA GGCGATGTGG AAGCTGAAGC AGTATCAGTG TCGAGTTTTA 
ATATCCACTG ATTTGACGTC ACGTGGAATT GATGCTGAGA AGGTGAACTT GGTGATTAAT 
CTGGACGTTC CTCAGGACTG GGAGACCTAC ATGCACCGTA TTGGTCGTGC TGGACGTTTT 
GGTACTCTGG GGGTTGCCGT GACCTACTGT TGCCATGGGG AGGAGGAGAA CAAGATGATG 
GCCATCGCTC AGAAGTGCTC TCTGGATCTC ATGCATTTAC CAGATCCAAT ACCTCCAGGC 
ATCATGGAAG AAGCGTGTGA CCATGATGTG ATCATAGAAA CTCTTTCAAA ACCAAAGATT 
CCTGACAATT TCAAACCAGA GAAGAAGAAA AAGGCTAAAT CAGAACAGAT GAAGTCTAAA 
CCCTCTAGAG AGTCTCACAT CTCTGTGGTC ATCACAGAAA GCGTGACCGG TGACAAATCA 
TGCAGTCGAG CTGAAACCAA GACTAAAAAT CATTCTTCCA AACCTGGGAG GCAAAAACAA 
ATGGCGCCTG CTGCAAACCT CCAACATGAC TCGACTATTA CTCAGAAACA GCAGAATGAC 
ACCCTTCCCA AAATTCCTCC TTTGTCATCC TTTAAGTCGC ACACAGGGAA GAGAATAACC 
TTCGACGATG CTTTGGTGGA TTATGAGACC TTTATAAGAG AAGGTCCGGG AAGATCTGTT 
GAAATCATTC ACCAGTATGA TGGTTTCTAC AGGAGTCAAG ATAAAGTTAA TGGTCTTCAT 
GACGCCCATG ATGAAGATGA GGCTTCTGAA TCATACGTTC TAGAAGAATC ACAGTCCCAT 
CAAGATCTTC CACCAGCGCA GGAGGTTTCA GAGTTGTCCT CAGAGCAGAA GACATCTAGT 
GAATCCTCTT CTGAGTCTGA GATGGAAGTG GAGAGTGAAT CAAGCTCTTC TGTGCCTAGC 
AAGTCTACAC TTGAAGCACT TCCAGATAAT AAAGTTTCGC TTGTACGTAA TCATTCAGAA 
TTAGCTGAAT TAGCTTTACC TTCAACCAGA GTACCACAAC AGGCCACCAG AAGCAAGAAA 
AGGCCATGTC AGCCTCAATC CCAGGACACC GCTCACCATC ACAATCTTCC TCATAAACAC 
CGAACAGCCT CCAAATCTAG CCGGAGGCCA ACAGGACCAA AGCGGAGAAC AAGAGAGACT 
GATGAGACTG AGGAGGAAGA GGCTGGACAG GATTATTGGA GCTCGTACAG GGCCTGGGCT 
GAATATTACA ATTCATATTA TCACCATTCG CCCTATAATT GGATGACTGC GTTTTATATG 
AATTCTGTGT ACATAAAGGA AATGATGAAG CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302268.1
CDS	50..2485
Translation	MPIMSVCRPLQDVARYSRQRFCMCAQEGAVIHSSCHLFTVEETERCRDRNMAASVKAAHELQSRTRTDDVLISGGVEFSSLLLSKPVLEGLSASGFQRPSPIQLKAIPLGRCGLDLIVQAKSGTGKTCVFTTIALDSLILENATTQVLVLAPTREIAVQIHAVVMAIGSAMEGLECHVFIGGRPISQDKQHLKKCHIAIGSPGRIKQLIEMGALMVSSIRLFVLDEADKLLEDDSSSSFQEQINWIYSSLPANKQMLALSATYPESLAQQLSRYMREPTFVRLNPTDPGLLGLKQYYKIVPSHSLPHKVFEEKVQSLLELFSKIPFNQALVFSNLHTRAQHLADILSSKGLPAVCISGGLSQDQRLEAMWKLKQYQCRVLISTDLTSRGIDAEKVNLVINLDVPQDWETYMHRIGRAGRFGTLGVAVTYCCHGEEENKMMAIAQKCSLDLMHLPDPIPPGIMEEACDHDVIIETLSKPKIPDNFKPEKKKKAKSEQMKSKPSRESHISVVITESVTGDKSCSRAETKTKNHSSKPGRQKQMAPAANLQHDSTITQKQQNDTLPKIPPLSSFKSHTGKRITFDDALVDYETFIREGPGRSVEIIHQYDGFYRSQDKVNGLHDAHDEDEASESYVLEESQSHQDLPPAQEVSELSSEQKTSSESSSESEMEVESESSSSVPSKSTLEALPDNKVSLVRNHSELAELALPSTRVPQQATRSKKRPCQPQSQDTAHHHNLPHKHRTASKSSRRPTGPKRRTRETDETEEEEAGQDYWSSYRAWAEYYNSYYHHSPYNWMTAFYMNSVYIKEMMKH

Target ORF information:

RefSeq Version	XM_009303993.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303993.2

ORF Insert Sequence:

ATGCCAATCA TGAGTGTTTG TCGCCCTCTG CAGGACGTGG CTCGATATTC CAGGCAGCGG 
TTCTGTATGT GTGCGCAGGA GGGCGCAGTG ATTCACAGCT CATGTCATTT ATTCACCGTA 
GAAGAAACAG AACGTTGTCG CGATAGAAAC ATGGCGGCAT CTGTGAAAGC AGCGCATGAG 
CTGCAATCGA GAACCCGGAC GGATGATGTT TTGATTAGCG GCGGTGTCGA GTTCAGCTCT 
TTACTCCTCT CAAAACCCGT GCTTGAGGGT TTATCTGCTT CCGGTTTTCA GAGACCCTCT 
CCCATCCAGC TAAAGGCAAT CCCGCTGGGA AGATGTGGAC TCGATCTAAT TGTTCAAGCC 
AAATCTGGCA CAGGAAAGAC CTGTGTGTTC ACCACCATCG CCTTGGACTC TCTTATATTG 
GAAAACGCAA CTACACAGGT GCTGGTTTTG GCCCCCACTC GGGAGATTGC AGTGCAGATT 
CACGCTGTTG TCATGGCGAT AGGCAGTGCT ATGGAGGGTT TGGAGTGCCA TGTGTTCATT 
GGTGGTCGAC CTATCAGTCA AGATAAACAG CACCTGAAGA AGTGCCACAT TGCTATTGGG 
TCACCAGGTC GTATTAAGCA GCTAATTGAG ATGGGAGCCC TGATGGTGTC CTCCATTCGC 
TTGTTTGTTT TGGATGAGGC TGATAAACTC CTGGAGGACG ACAGCAGCAG CAGCTTTCAG 
GAGCAGATCA ACTGGATCTA CTCCTCTCTA CCAGCAAATA AACAAATGTT GGCTCTTTCT 
GCCACCTACC CAGAGTCTTT AGCACAACAG TTGTCAAGAT ACATGAGAGA GCCCACGTTT 
GTGCGACTGA ACCCAACTGA CCCTGGACTC CTCGGACTGA AGCAGTATTA TAAGATTGTG 
CCATCTCATT CATTGCCTCA TAAAGTTTTT GAGGAGAAGG TGCAGAGTCT TCTTGAACTC 
TTCAGTAAAA TCCCATTTAA CCAAGCGCTG GTGTTCTCGA ATCTTCATAC CAGAGCTCAG 
CATCTGGCAG ATATCCTCTC CTCTAAAGGT TTGCCTGCAG TCTGCATCTC AGGTGGTTTA 
AGTCAGGATC AGAGGTTAGA GGCGATGTGG AAGCTGAAGC AGTATCAGTG TCGAGTTTTA 
ATATCCACTG ATTTGACGTC ACGTGGAATT GATGCTGAGA AGGTGAACTT GGTGATTAAT 
CTGGACGTTC CTCAGGACTG GGAGACCTAC ATGCACCGTA TTGGTCGTGC TGGACGTTTT 
GGTACTCTGG GGGTTGCCGT GACCTACTGT TGCCATGGGG AGGAGGAGAA CAAGATGATG 
GCCATCGCTC AGAAGTGCTC TCTGGATCTC ATGCATTTAC CAGATCCAAT ACCTCCAGGC 
ATCATGGAAG AAGCGTGTGA CCATGATGTG ATCATAGAAA CTCTTTCAAA ACCAAAGATT 
CCTGACAATT TCAAACCAGA GAAGAAGAAA AAGGCTAAAT CAGAACAGAT GAAGTCTAAA 
CCCTCTAGAG AGTCTCACAT CTCTGTGGTC ATCACAGAAA GCGTGACCGG TGACAAATCA 
TGCAGTCGAG CTGAAACCAA GACTAAAAAT CATTCTTCCA AACCTGGGAG GCAAAAACAA 
ATGGCGCCTG CTGCAAACCT CCAACATGAC TCGACTATTA CTCAGAAACA GCAGAATGAC 
ACCCTTCCCA AAATTCCTCC TTTGTCATCC TTTAAGTCGC ACACAGGGAA GAGAATAACC 
TTCGACGATG CTTTGGTGGA TTATGAGACC TTTATAAGAG AAGGTCCGGG AAGATCTGTT 
GAAATCATTC ACCAGTATGA TGGTTTCTAC AGGAGTCAAG ATAAAGTTAA TGGTCTTCAT 
GACGCCCATG ATGAAGATGA GGCTTCTGAA TCATACGTTC TAGAAGAATC ACAGTCCCAT 
CAAGATCTTC CACCAGCGCA GGAGGTTTCA GAGTTGTCCT CAGAGCAGAA GACATCTAGT 
GAATCCTCTT CTGAGTCTGA GATGGAAGTG GAGAGTGAAT CAAGCTCTTC TGTGCCTAGC 
AAGTCTACAC TTGAAGCACT TCCAGATAAT AAAGTTTCGC TTGTACGTAA TCATTCAGAA 
TTAGCTGAAT TAGCTTTACC TTCAACCAGA GTACCACAAC AGGCCACCAG AAGCAAGAAA 
AGGCCATGTC AGCCTCAATC CCAGGACACC GCTCACCATC ACAATCTTCC TCATAAACAC 
CGAACAGCCT CCAAATCTAG CCGGAGGCCA ACAGGACCAA AGCGGAGAAC AAGAGAGACT 
GATGAGACTG AGGAGGAAGA GGCTGGACAG GATTATTGGA GCTCGTACAG GGCCTGGGCT 
GAATATTACA ATTCATATTA TCACCATTCG CCCTATAATT GGATGACTGC GTTTTATATG 
AATTCTGTGT ACATAAAGGA AATGATGAAG CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx20 ( XM_009303993.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302268.1 Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42332
Clone ID Related Accession (Same CDS sequence)	XM_009303993.2 , XM_009303993.3
Accession Version	XM_009303993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable ATP-dependent RNA helicase DDX20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAATCA TGAGTGTTTG TCGCCCTCTG CAGGACGTGG CTCGATATTC CAGGCAGCGG 
TTCTGTATGT GTGCGCAGGA GGGCGCAGTG ATTCACAGCT CATGTCATTT ATTCACCGTA 
GAAGAAACAG AACGTTGTCG CGATAGAAAC ATGGCGGCAT CTGTGAAAGC AGCGCATGAG 
CTGCAATCGA GAACCCGGAC GGATGATGTT TTGATTAGCG GCGGTGTCGA GTTCAGCTCT 
TTACTCCTCT CAAAACCCGT GCTTGAGGGT TTATCTGCTT CCGGTTTTCA GAGACCCTCT 
CCCATCCAGC TAAAGGCAAT CCCGCTGGGA AGATGTGGAC TCGATCTAAT TGTTCAAGCC 
AAATCTGGCA CAGGAAAGAC CTGTGTGTTC ACCACCATCG CCTTGGACTC TCTTATATTG 
GAAAACGCAA CTACACAGGT GCTGGTTTTG GCCCCCACTC GGGAGATTGC AGTGCAGATT 
CACGCTGTTG TCATGGCGAT AGGCAGTGCT ATGGAGGGTT TGGAGTGCCA TGTGTTCATT 
GGTGGTCGAC CTATCAGTCA AGATAAACAG CACCTGAAGA AGTGCCACAT TGCTATTGGG 
TCACCAGGTC GTATTAAGCA GCTAATTGAG ATGGGAGCCC TGATGGTGTC CTCCATTCGC 
TTGTTTGTTT TGGATGAGGC TGATAAACTC CTGGAGGACG ACAGCAGCAG CAGCTTTCAG 
GAGCAGATCA ACTGGATCTA CTCCTCTCTA CCAGCAAATA AACAAATGTT GGCTCTTTCT 
GCCACCTACC CAGAGTCTTT AGCACAACAG TTGTCAAGAT ACATGAGAGA GCCCACGTTT 
GTGCGACTGA ACCCAACTGA CCCTGGACTC CTCGGACTGA AGCAGTATTA TAAGATTGTG 
CCATCTCATT CATTGCCTCA TAAAGTTTTT GAGGAGAAGG TGCAGAGTCT TCTTGAACTC 
TTCAGTAAAA TCCCATTTAA CCAAGCGCTG GTGTTCTCGA ATCTTCATAC CAGAGCTCAG 
CATCTGGCAG ATATCCTCTC CTCTAAAGGT TTGCCTGCAG TCTGCATCTC AGGTGGTTTA 
AGTCAGGATC AGAGGTTAGA GGCGATGTGG AAGCTGAAGC AGTATCAGTG TCGAGTTTTA 
ATATCCACTG ATTTGACGTC ACGTGGAATT GATGCTGAGA AGGTGAACTT GGTGATTAAT 
CTGGACGTTC CTCAGGACTG GGAGACCTAC ATGCACCGTA TTGGTCGTGC TGGACGTTTT 
GGTACTCTGG GGGTTGCCGT GACCTACTGT TGCCATGGGG AGGAGGAGAA CAAGATGATG 
GCCATCGCTC AGAAGTGCTC TCTGGATCTC ATGCATTTAC CAGATCCAAT ACCTCCAGGC 
ATCATGGAAG AAGCGTGTGA CCATGATGTG ATCATAGAAA CTCTTTCAAA ACCAAAGATT 
CCTGACAATT TCAAACCAGA GAAGAAGAAA AAGGCTAAAT CAGAACAGAT GAAGTCTAAA 
CCCTCTAGAG AGTCTCACAT CTCTGTGGTC ATCACAGAAA GCGTGACCGG TGACAAATCA 
TGCAGTCGAG CTGAAACCAA GACTAAAAAT CATTCTTCCA AACCTGGGAG GCAAAAACAA 
ATGGCGCCTG CTGCAAACCT CCAACATGAC TCGACTATTA CTCAGAAACA GCAGAATGAC 
ACCCTTCCCA AAATTCCTCC TTTGTCATCC TTTAAGTCGC ACACAGGGAA GAGAATAACC 
TTCGACGATG CTTTGGTGGA TTATGAGACC TTTATAAGAG AAGGTCCGGG AAGATCTGTT 
GAAATCATTC ACCAGTATGA TGGTTTCTAC AGGAGTCAAG ATAAAGTTAA TGGTCTTCAT 
GACGCCCATG ATGAAGATGA GGCTTCTGAA TCATACGTTC TAGAAGAATC ACAGTCCCAT 
CAAGATCTTC CACCAGCGCA GGAGGTTTCA GAGTTGTCCT CAGAGCAGAA GACATCTAGT 
GAATCCTCTT CTGAGTCTGA GATGGAAGTG GAGAGTGAAT CAAGCTCTTC TGTGCCTAGC 
AAGTCTACAC TTGAAGCACT TCCAGATAAT AAAGTTTCGC TTGTACGTAA TCATTCAGAA 
TTAGCTGAAT TAGCTTTACC TTCAACCAGA GTACCACAAC AGGCCACCAG AAGCAAGAAA 
AGGCCATGTC AGCCTCAATC CCAGGACACC GCTCACCATC ACAATCTTCC TCATAAACAC 
CGAACAGCCT CCAAATCTAG CCGGAGGCCA ACAGGACCAA AGCGGAGAAC AAGAGAGACT 
GATGAGACTG AGGAGGAAGA GGCTGGACAG GATTATTGGA GCTCGTACAG GGCCTGGGCT 
GAATATTACA ATTCATATTA TCACCATTCG CCCTATAATT GGATGACTGC GTTTTATATG 
AATTCTGTGT ACATAAAGGA AATGATGAAG CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302268.1
CDS	34..2469
Translation	MPIMSVCRPLQDVARYSRQRFCMCAQEGAVIHSSCHLFTVEETERCRDRNMAASVKAAHELQSRTRTDDVLISGGVEFSSLLLSKPVLEGLSASGFQRPSPIQLKAIPLGRCGLDLIVQAKSGTGKTCVFTTIALDSLILENATTQVLVLAPTREIAVQIHAVVMAIGSAMEGLECHVFIGGRPISQDKQHLKKCHIAIGSPGRIKQLIEMGALMVSSIRLFVLDEADKLLEDDSSSSFQEQINWIYSSLPANKQMLALSATYPESLAQQLSRYMREPTFVRLNPTDPGLLGLKQYYKIVPSHSLPHKVFEEKVQSLLELFSKIPFNQALVFSNLHTRAQHLADILSSKGLPAVCISGGLSQDQRLEAMWKLKQYQCRVLISTDLTSRGIDAEKVNLVINLDVPQDWETYMHRIGRAGRFGTLGVAVTYCCHGEEENKMMAIAQKCSLDLMHLPDPIPPGIMEEACDHDVIIETLSKPKIPDNFKPEKKKKAKSEQMKSKPSRESHISVVITESVTGDKSCSRAETKTKNHSSKPGRQKQMAPAANLQHDSTITQKQQNDTLPKIPPLSSFKSHTGKRITFDDALVDYETFIREGPGRSVEIIHQYDGFYRSQDKVNGLHDAHDEDEASESYVLEESQSHQDLPPAQEVSELSSEQKTSSESSSESEMEVESESSSSVPSKSTLEALPDNKVSLVRNHSELAELALPSTRVPQQATRSKKRPCQPQSQDTAHHHNLPHKHRTASKSSRRPTGPKRRTRETDETEEEEAGQDYWSSYRAWAEYYNSYYHHSPYNWMTAFYMNSVYIKEMMKH

Target ORF information:

RefSeq Version	XM_009303993.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303993.3

ORF Insert Sequence:

ATGCCAATCA TGAGTGTTTG TCGCCCTCTG CAGGACGTGG CTCGATATTC CAGGCAGCGG 
TTCTGTATGT GTGCGCAGGA GGGCGCAGTG ATTCACAGCT CATGTCATTT ATTCACCGTA 
GAAGAAACAG AACGTTGTCG CGATAGAAAC ATGGCGGCAT CTGTGAAAGC AGCGCATGAG 
CTGCAATCGA GAACCCGGAC GGATGATGTT TTGATTAGCG GCGGTGTCGA GTTCAGCTCT 
TTACTCCTCT CAAAACCCGT GCTTGAGGGT TTATCTGCTT CCGGTTTTCA GAGACCCTCT 
CCCATCCAGC TAAAGGCAAT CCCGCTGGGA AGATGTGGAC TCGATCTAAT TGTTCAAGCC 
AAATCTGGCA CAGGAAAGAC CTGTGTGTTC ACCACCATCG CCTTGGACTC TCTTATATTG 
GAAAACGCAA CTACACAGGT GCTGGTTTTG GCCCCCACTC GGGAGATTGC AGTGCAGATT 
CACGCTGTTG TCATGGCGAT AGGCAGTGCT ATGGAGGGTT TGGAGTGCCA TGTGTTCATT 
GGTGGTCGAC CTATCAGTCA AGATAAACAG CACCTGAAGA AGTGCCACAT TGCTATTGGG 
TCACCAGGTC GTATTAAGCA GCTAATTGAG ATGGGAGCCC TGATGGTGTC CTCCATTCGC 
TTGTTTGTTT TGGATGAGGC TGATAAACTC CTGGAGGACG ACAGCAGCAG CAGCTTTCAG 
GAGCAGATCA ACTGGATCTA CTCCTCTCTA CCAGCAAATA AACAAATGTT GGCTCTTTCT 
GCCACCTACC CAGAGTCTTT AGCACAACAG TTGTCAAGAT ACATGAGAGA GCCCACGTTT 
GTGCGACTGA ACCCAACTGA CCCTGGACTC CTCGGACTGA AGCAGTATTA TAAGATTGTG 
CCATCTCATT CATTGCCTCA TAAAGTTTTT GAGGAGAAGG TGCAGAGTCT TCTTGAACTC 
TTCAGTAAAA TCCCATTTAA CCAAGCGCTG GTGTTCTCGA ATCTTCATAC CAGAGCTCAG 
CATCTGGCAG ATATCCTCTC CTCTAAAGGT TTGCCTGCAG TCTGCATCTC AGGTGGTTTA 
AGTCAGGATC AGAGGTTAGA GGCGATGTGG AAGCTGAAGC AGTATCAGTG TCGAGTTTTA 
ATATCCACTG ATTTGACGTC ACGTGGAATT GATGCTGAGA AGGTGAACTT GGTGATTAAT 
CTGGACGTTC CTCAGGACTG GGAGACCTAC ATGCACCGTA TTGGTCGTGC TGGACGTTTT 
GGTACTCTGG GGGTTGCCGT GACCTACTGT TGCCATGGGG AGGAGGAGAA CAAGATGATG 
GCCATCGCTC AGAAGTGCTC TCTGGATCTC ATGCATTTAC CAGATCCAAT ACCTCCAGGC 
ATCATGGAAG AAGCGTGTGA CCATGATGTG ATCATAGAAA CTCTTTCAAA ACCAAAGATT 
CCTGACAATT TCAAACCAGA GAAGAAGAAA AAGGCTAAAT CAGAACAGAT GAAGTCTAAA 
CCCTCTAGAG AGTCTCACAT CTCTGTGGTC ATCACAGAAA GCGTGACCGG TGACAAATCA 
TGCAGTCGAG CTGAAACCAA GACTAAAAAT CATTCTTCCA AACCTGGGAG GCAAAAACAA 
ATGGCGCCTG CTGCAAACCT CCAACATGAC TCGACTATTA CTCAGAAACA GCAGAATGAC 
ACCCTTCCCA AAATTCCTCC TTTGTCATCC TTTAAGTCGC ACACAGGGAA GAGAATAACC 
TTCGACGATG CTTTGGTGGA TTATGAGACC TTTATAAGAG AAGGTCCGGG AAGATCTGTT 
GAAATCATTC ACCAGTATGA TGGTTTCTAC AGGAGTCAAG ATAAAGTTAA TGGTCTTCAT 
GACGCCCATG ATGAAGATGA GGCTTCTGAA TCATACGTTC TAGAAGAATC ACAGTCCCAT 
CAAGATCTTC CACCAGCGCA GGAGGTTTCA GAGTTGTCCT CAGAGCAGAA GACATCTAGT 
GAATCCTCTT CTGAGTCTGA GATGGAAGTG GAGAGTGAAT CAAGCTCTTC TGTGCCTAGC 
AAGTCTACAC TTGAAGCACT TCCAGATAAT AAAGTTTCGC TTGTACGTAA TCATTCAGAA 
TTAGCTGAAT TAGCTTTACC TTCAACCAGA GTACCACAAC AGGCCACCAG AAGCAAGAAA 
AGGCCATGTC AGCCTCAATC CCAGGACACC GCTCACCATC ACAATCTTCC TCATAAACAC 
CGAACAGCCT CCAAATCTAG CCGGAGGCCA ACAGGACCAA AGCGGAGAAC AAGAGAGACT 
GATGAGACTG AGGAGGAAGA GGCTGGACAG GATTATTGGA GCTCGTACAG GGCCTGGGCT 
GAATATTACA ATTCATATTA TCACCATTCG CCCTATAATT GGATGACTGC GTTTTATATG 
AATTCTGTGT ACATAAAGGA AATGATGAAG CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx20 ( XM_021478262.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333937.1 Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa14223
Clone ID Related Accession (Same CDS sequence)	XM_021478262.1
Accession Version	XM_021478262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable ATP-dependent RNA helicase DDX20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGCGC AGGAGGGCGC AGTGATTCAC AGCTCATGTC ATTTATTCAC CGTAGAAGAA 
ACAGAACGTT GTCGCGATAG AAACATGGCG GCATCTGTGA AAGCAGCGCA TGAGCTGCAA 
TCGAGAACCC GGACGGATGA TGTTTTGATT AGCGGCGGTG TCGAGTTCAG CTCTTTACTC 
CTCTCAAAAC CCGTGCTTGA GGGTTTATCT GCTTCCGGTT TTCAGAGACC CTCTCCCATC 
CAGCTAAAGG CAATCCCGCT GGGAAGATGT GGACTCGATC TAATTGTTCA AGCCAAATCT 
GGCACAGGAA AGACCTGTGT GTTCACCACC ATCGCCTTGG ACTCTCTTAT ATTGGAAAAC 
GCAACTACAC AGGTGCTGGT TTTGGCCCCC ACTCGGGAGA TTGCAGTGCA GATTCACGCT 
GTTGTCATGG CGATAGGCAG TGCTATGGAG GGTTTGGAGT GCCATGTGTT CATTGGTGGT 
CGACCTATCA GTCAAGATAA ACAGCACCTG AAGAAGTGCC ACATTGCTAT TGGGTCACCA 
GGTCGTATTA AGCAGCTAAT TGAGATGGGA GCCCTGATGG TGTCCTCCAT TCGCTTGTTT 
GTTTTGGATG AGGCTGATAA ACTCCTGGAG GACGACAGCA GCAGCAGCTT TCAGGAGCAG 
ATCAACTGGA TCTACTCCTC TCTACCAGCA AATAAACAAA TGTTGGCTCT TTCTGCCACC 
TACCCAGAGT CTTTAGCACA ACAGTTGTCA AGATACATGA GAGAGCCCAC GTTTGTGCGA 
CTGAACCCAA CTGACCCTGG ACTCCTCGGA CTGAAGCAGT ATTATAAGAT TGTGCCATCT 
CATTCATTGC CTCATAAAGT TTTTGAGGAG AAGGTGCAGA GTCTTCTTGA ACTCTTCAGT 
AAAATCCCAT TTAACCAAGC GCTGGTGTTC TCGAATCTTC ATACCAGAGC TCAGCATCTG 
GCAGATATCC TCTCCTCTAA AGGTTTGCCT GCAGTCTGCA TCTCAGGTGG TTTAAGTCAG 
GATCAGAGGT TAGAGGCGAT GTGGAAGCTG AAGCAGTATC AGTGTCGAGT TTTAATATCC 
ACTGATTTGA CGTCACGTGG AATTGATGCT GAGAAGGTGA ACTTGGTGAT TAATCTGGAC 
GTTCCTCAGG ACTGGGAGAC CTACATGCAC CGTATTGGTC GTGCTGGACG TTTTGGTACT 
CTGGGGGTTG CCGTGACCTA CTGTTGCCAT GGGGAGGAGG AGAACAAGAT GATGGCCATC 
GCTCAGAAGT GCTCTCTGGA TCTCATGCAT TTACCAGATC CAATACCTCC AGGCATCATG 
GAAGAAGCGT GTGACCATGA TGTGATCATA GAAACTCTTT CAAAACCAAA GATTCCTGAC 
AATTTCAAAC CAGAGAAGAA GAAAAAGGCT AAATCAGAAC AGATGAAGTC TAAACCCTCT 
AGAGAGTCTC ACATCTCTGT GGTCATCACA GAAAGCGTGA CCGGTGACAA ATCATGCAGT 
CGAGCTGAAA CCAAGACTAA AAATCATTCT TCCAAACCTG GGAGGCAAAA ACAAATGGCG 
CCTGCTGCAA ACCTCCAACA TGACTCGACT ATTACTCAGA AACAGCAGAA TGACACCCTT 
CCCAAAATTC CTCCTTTGTC ATCCTTTAAG TCGCACACAG GGAAGAGAAT AACCTTCGAC 
GATGCTTTGG TGGATTATGA GACCTTTATA AGAGAAGGTC CGGGAAGATC TGTTGAAATC 
ATTCACCAGT ATGATGGTTT CTACAGGAGT CAAGATAAAG TTAATGGTCT TCATGACGCC 
CATGATGAAG ATGAGGCTTC TGAATCATAC GTTCTAGAAG AATCACAGTC CCATCAAGAT 
CTTCCACCAG CGCAGGAGGT TTCAGAGTTG TCCTCAGAGC AGAAGACATC TAGTGAATCC 
TCTTCTGAGT CTGAGATGGA AGTGGAGAGT GAATCAAGCT CTTCTGTGCC TAGCAAGTCT 
ACACTTGAAG CACTTCCAGA TAATAAAGTT TCGCTTGTAC GTAATCATTC AGAATTAGCT 
GAATTAGCTT TACCTTCAAC CAGAGTACCA CAACAGGCCA CCAGAAGCAA GAAAAGGCCA 
TGTCAGCCTC AATCCCAGGA CACCGCTCAC CATCACAATC TTCCTCATAA ACACCGAACA 
GCCTCCAAAT CTAGCCGGAG GCCAACAGGA CCAAAGCGGA GAACAAGAGA GACTGATGAG 
ACTGAGGAGG AAGAGGCTGG ACAGGATTAT TGGAGCTCGT ACAGGGCCTG GGCTGAATAT 
TACAATTCAT ATTATCACCA TTCGCCCTAT AATTGGATGA CTGCGTTTTA TATGAATTCT 
GTGTACATAA AGGAAATGAT GAAGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333937.1
CDS	425..2794
Translation	MCAQEGAVIHSSCHLFTVEETERCRDRNMAASVKAAHELQSRTRTDDVLISGGVEFSSLLLSKPVLEGLSASGFQRPSPIQLKAIPLGRCGLDLIVQAKSGTGKTCVFTTIALDSLILENATTQVLVLAPTREIAVQIHAVVMAIGSAMEGLECHVFIGGRPISQDKQHLKKCHIAIGSPGRIKQLIEMGALMVSSIRLFVLDEADKLLEDDSSSSFQEQINWIYSSLPANKQMLALSATYPESLAQQLSRYMREPTFVRLNPTDPGLLGLKQYYKIVPSHSLPHKVFEEKVQSLLELFSKIPFNQALVFSNLHTRAQHLADILSSKGLPAVCISGGLSQDQRLEAMWKLKQYQCRVLISTDLTSRGIDAEKVNLVINLDVPQDWETYMHRIGRAGRFGTLGVAVTYCCHGEEENKMMAIAQKCSLDLMHLPDPIPPGIMEEACDHDVIIETLSKPKIPDNFKPEKKKKAKSEQMKSKPSRESHISVVITESVTGDKSCSRAETKTKNHSSKPGRQKQMAPAANLQHDSTITQKQQNDTLPKIPPLSSFKSHTGKRITFDDALVDYETFIREGPGRSVEIIHQYDGFYRSQDKVNGLHDAHDEDEASESYVLEESQSHQDLPPAQEVSELSSEQKTSSESSSESEMEVESESSSSVPSKSTLEALPDNKVSLVRNHSELAELALPSTRVPQQATRSKKRPCQPQSQDTAHHHNLPHKHRTASKSSRRPTGPKRRTRETDETEEEEAGQDYWSSYRAWAEYYNSYYHHSPYNWMTAFYMNSVYIKEMMKH

Target ORF information:

RefSeq Version	XM_021478262.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (ddx20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478262.1

ORF Insert Sequence:

ATGTGTGCGC AGGAGGGCGC AGTGATTCAC AGCTCATGTC ATTTATTCAC CGTAGAAGAA 
ACAGAACGTT GTCGCGATAG AAACATGGCG GCATCTGTGA AAGCAGCGCA TGAGCTGCAA 
TCGAGAACCC GGACGGATGA TGTTTTGATT AGCGGCGGTG TCGAGTTCAG CTCTTTACTC 
CTCTCAAAAC CCGTGCTTGA GGGTTTATCT GCTTCCGGTT TTCAGAGACC CTCTCCCATC 
CAGCTAAAGG CAATCCCGCT GGGAAGATGT GGACTCGATC TAATTGTTCA AGCCAAATCT 
GGCACAGGAA AGACCTGTGT GTTCACCACC ATCGCCTTGG ACTCTCTTAT ATTGGAAAAC 
GCAACTACAC AGGTGCTGGT TTTGGCCCCC ACTCGGGAGA TTGCAGTGCA GATTCACGCT 
GTTGTCATGG CGATAGGCAG TGCTATGGAG GGTTTGGAGT GCCATGTGTT CATTGGTGGT 
CGACCTATCA GTCAAGATAA ACAGCACCTG AAGAAGTGCC ACATTGCTAT TGGGTCACCA 
GGTCGTATTA AGCAGCTAAT TGAGATGGGA GCCCTGATGG TGTCCTCCAT TCGCTTGTTT 
GTTTTGGATG AGGCTGATAA ACTCCTGGAG GACGACAGCA GCAGCAGCTT TCAGGAGCAG 
ATCAACTGGA TCTACTCCTC TCTACCAGCA AATAAACAAA TGTTGGCTCT TTCTGCCACC 
TACCCAGAGT CTTTAGCACA ACAGTTGTCA AGATACATGA GAGAGCCCAC GTTTGTGCGA 
CTGAACCCAA CTGACCCTGG ACTCCTCGGA CTGAAGCAGT ATTATAAGAT TGTGCCATCT 
CATTCATTGC CTCATAAAGT TTTTGAGGAG AAGGTGCAGA GTCTTCTTGA ACTCTTCAGT 
AAAATCCCAT TTAACCAAGC GCTGGTGTTC TCGAATCTTC ATACCAGAGC TCAGCATCTG 
GCAGATATCC TCTCCTCTAA AGGTTTGCCT GCAGTCTGCA TCTCAGGTGG TTTAAGTCAG 
GATCAGAGGT TAGAGGCGAT GTGGAAGCTG AAGCAGTATC AGTGTCGAGT TTTAATATCC 
ACTGATTTGA CGTCACGTGG AATTGATGCT GAGAAGGTGA ACTTGGTGAT TAATCTGGAC 
GTTCCTCAGG ACTGGGAGAC CTACATGCAC CGTATTGGTC GTGCTGGACG TTTTGGTACT 
CTGGGGGTTG CCGTGACCTA CTGTTGCCAT GGGGAGGAGG AGAACAAGAT GATGGCCATC 
GCTCAGAAGT GCTCTCTGGA TCTCATGCAT TTACCAGATC CAATACCTCC AGGCATCATG 
GAAGAAGCGT GTGACCATGA TGTGATCATA GAAACTCTTT CAAAACCAAA GATTCCTGAC 
AATTTCAAAC CAGAGAAGAA GAAAAAGGCT AAATCAGAAC AGATGAAGTC TAAACCCTCT 
AGAGAGTCTC ACATCTCTGT GGTCATCACA GAAAGCGTGA CCGGTGACAA ATCATGCAGT 
CGAGCTGAAA CCAAGACTAA AAATCATTCT TCCAAACCTG GGAGGCAAAA ACAAATGGCG 
CCTGCTGCAA ACCTCCAACA TGACTCGACT ATTACTCAGA AACAGCAGAA TGACACCCTT 
CCCAAAATTC CTCCTTTGTC ATCCTTTAAG TCGCACACAG GGAAGAGAAT AACCTTCGAC 
GATGCTTTGG TGGATTATGA GACCTTTATA AGAGAAGGTC CGGGAAGATC TGTTGAAATC 
ATTCACCAGT ATGATGGTTT CTACAGGAGT CAAGATAAAG TTAATGGTCT TCATGACGCC 
CATGATGAAG ATGAGGCTTC TGAATCATAC GTTCTAGAAG AATCACAGTC CCATCAAGAT 
CTTCCACCAG CGCAGGAGGT TTCAGAGTTG TCCTCAGAGC AGAAGACATC TAGTGAATCC 
TCTTCTGAGT CTGAGATGGA AGTGGAGAGT GAATCAAGCT CTTCTGTGCC TAGCAAGTCT 
ACACTTGAAG CACTTCCAGA TAATAAAGTT TCGCTTGTAC GTAATCATTC AGAATTAGCT 
GAATTAGCTT TACCTTCAAC CAGAGTACCA CAACAGGCCA CCAGAAGCAA GAAAAGGCCA 
TGTCAGCCTC AATCCCAGGA CACCGCTCAC CATCACAATC TTCCTCATAA ACACCGAACA 
GCCTCCAAAT CTAGCCGGAG GCCAACAGGA CCAAAGCGGA GAACAAGAGA GACTGATGAG 
ACTGAGGAGG AAGAGGCTGG ACAGGATTAT TGGAGCTCGT ACAGGGCCTG GGCTGAATAT 
TACAATTCAT ATTATCACCA TTCGCCCTAT AATTGGATGA CTGCGTTTTA TATGAATTCT 
GTGTACATAA AGGAAATGAT GAAGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Forward Genetic Screens in Zebrafish Identify Pre-mRNA-Processing Pathways Regulating Early T Cell Development.
Cell reports17(9)2259-2270(2016 11)
Iwanami N,Sikora K,Richter AS,M?nnich M,Guerri L,Soza-Ried C,Lawir DF,Mateos F,Hess I,O'Meara CP,Schorpp M,Boehm T

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Processing of fish Ig heavy chain transcripts: diverse splicing patterns and unusual nonsense mediated decay.
Developmental and comparative immunology35(9)949-58(2011 Sep)
Quiniou SM,Wilson M,Boudinot P

Global repression of cancer gene expression in a zebrafish model of melanoma is linked to epigenetic regulation.
Zebrafish6(4)417-24(2009 Dec)
Anelli V,Santoriello C,Distel M,K?ster RW,Ciccarelli FD,Mione M

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ddx20 cDNA ORF clone, Danio rerio(zebrafish)

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