Ngef cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ngef
Entrez Gene ID	53972
Full Name	neuronal guanine nucleotide exchange factor
Synonyms	Tims2,ephexin
General protein information	Preferred Names neuronal guanine nucleotide exchange factor Names ephexin-1 eph-interacting exchange protein transforming immortalised mammary epithelial sequence 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 D\|1 44.42 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001111314.1	NP_001104784.1	ephexin-1 isoform 1
NM_019867.2	NP_063920.2	ephexin-1 isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NGEF	XP_005635921.1
Danio rerio (zebrafish)	LOC569090	XP_697546.3
Xenopus tropicalis (tropical clawed frog)	ngef	XP_004917852.1
Bos taurus (cattle)	NGEF	NP_001070394.1
Rattus norvegicus (Norway rat)	Ngef	NP_001129713.1
Pan troglodytes (chimpanzee)	NGEF	XP_516157.3
Homo sapiens (human)	NGEF	NP_062824.2
	LOC713917	XP_001114604.1
Mus musculus (house mouse)	Ngef	NP_001104784.1
Gallus gallus (chicken)	NGEF	NP_001010841.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007452	XP_308411.4

Gene Ontology
Bibliography

Related articles in PubMed

Increased EphA4-ephexin1 signaling in the medial prefrontal cortex plays a role in depression-like phenotype.
Zhang JC, Yao W, Qu Y, Nakamura M, Dong C, Yang C, Ren Q, Ma M, Han M, Shirayama Y, Hayashi-Takagi A, Hashimoto K
Scientific reports7(1)7133(2017 Aug)

FoxP1 orchestration of ASD-relevant signaling pathways in the striatum.
Araujo DJ, Anderson AG, Berto S, Runnels W, Harper M, Ammanuel S, Rieger MA, Huang HC, Rajkovich K, Loerwald KW, Dekker JD, Tucker HO, Dougherty JD, Gibson JR, Konopka G
Genes & development29(20)2081-96(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An essential role is identified for rho guanine nucleotide exchange factor (RhoGEF) ephexin1 in the maturation of acetylcholine receptor clusters.
Title: Ephexin1 is required for structural maturation and neurotransmission at the neuromuscular junction.

FGFR-mediated phosphorylation of ephexin1 enhances the guanine nucleotide exchange activity toward RhoA without affecting the activity to Rac1 or Cdc42.
Title: Regulation of ephexin1, a guanine nucleotide exchange factor of Rho family GTPases, by fibroblast growth factor receptor-mediated tyrosine phosphorylation.

The following Ngef gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ngef cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21223	NM_001111314.1 Latest version!	Mus musculus neuronal guanine nucleotide exchange factor (Ngef), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu21224	NM_019867.2 Latest version!	Mus musculus neuronal guanine nucleotide exchange factor (Ngef), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ngef ( NM_001111314.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001104784.1 Mus musculus neuronal guanine nucleotide exchange factor (Ngef), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu21223
Clone ID Related Accession (Same CDS sequence)	NM_001111314.1
Accession Version	NM_001111314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	ephexin-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102564.7, AC157811.3, AK138433.1, AJ238898.1 and AI847102.1. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039279.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGACCA AAAACTCTGA AGACTGGGGG AAACCTCAGA GGAAATCAGA GAGCAGCTCA 
CGGAAAAGCA ATCATGGCCC AGCTGAGATG AGGCCTGCGT TACCTCCGGA GAACAGGGAG 
GCACCTGAGA CTGGCGAGGA AACTCAAAAC GAAGAACCCC GCCGCCTCAT CCCAATCCAG 
AGGCATTCCC TCTTCAATCG TGCTGTGAGA CACAGGCACA AGGCCAGGAG CACGTCTGAG 
CGGAGAGCCA GTGACCAAGC AGATTTGCCA AAAATGGGGA AATCTGTAAA TGAACGGTCG 
GCCTTCAATC TGCCCCAGGG GAGATTGCCT CCCTGGAGAA CACCAGCCCA GAGGGACACA 
GGAGCGCAGG AAGCCAGCGA GTCATCCTCC ACCCCTGGAA ATGGAACCAC ACCCGAGGAG 
TGCCCAGCCC TCACCGACAG CCCCACCACT CTCACGGAGG CCCTGCAGAT GATCCATCCC 
ATTCCCGCCG ACTCCTGGAG AAACCTCATT GAACAAATAG GGCTCCTGTA TCAAGAGTAT 
AGAGACAAAT CGACTCTCCA AGAAATTGAA ACACGGAGGC AGCAGGATGC AGAAATCCAA 
GGCAACTCCG ATGGGTCCCA GGTTGGGGAA GACGCTGGAG AGGAGGAGGA GGAGGAGGAG 
GAGGGAGAGG AGGAGGAGCT GGCCAGCCCT CCTGAGAGGA GAGCTCTGCC TCAGATCTGC 
CTGCTCAGCA ACCCCCACTC CAGGTTCAAC CTCTGGCAAG ACCTTCCTGA GATCCAGAGC 
AGTGGCGTGC TGGACATTCT CCAGCCGGAG GAGATCAGGC TGCAGGAGGC CATGTTTGAG 
TTGGTTACCT CTGAGGCCTC CTACTATAAG AGCCTGAACC TGCTGGTGTC GCACTTCATG 
GAGAACGAGC GTCTGAAGAA GATCCTGCAT CCATCTGAGG CCCACATCCT CTTTTCCAAT 
GTCCTGGATG TCATGGCTGT CAGTGAGCGG TTTTTGCTGG AGCTAGAGCA CCGCATGGAG 
GAGAACATTG TTATCTCGGA TGTGTGCGAC ATCGTGTACC GTTACGCAGC TGATCACTTC 
TCGGTCTATA TCACTTACGT CAGTAACCAG ACCTACCAGG AAAGGACATA CAAGCAGCTC 
CTACAGGAGA AGGCCGCTTT CCGGGAACTG ATCGCACAGT TGGAGCTGGA CCCCAAATGC 
AAGGGCCTGC CTTTCTCCTC CTTCCTCATC TTGCCTTTCC AGAGGATCAC GAGACTCAAG 
CTGCTGGTCC AGAATATCCT GAAGAGAGTG GAGGAGAGGT CTGAACGTGA AGGCACCGCC 
TTGGATGCCC ACAAGGAGCT AGAAATGGTG GTAAAGGCAT GCAATGAGGG TGTCCGGAAG 
ATGAGCCGCA CAGAACAGAT GATCAGCATT CAGAAGAAGA TGGAGTTCAA GATCAAGTCG 
GTACCCATCA TCTCACACTC CCGGTGGCTG CTGAAGCAGG GTGAGCTGCA GCAGATGTCC 
GGCCCCAAGA CCTCCCGCAC CCTGCGGACC AAGAAGCTCT TCAGAGAAAT TTACCTCTTC 
CTCTTCAATG ACCTGCTGGT GATCTGCCGG CAGATCCCTG GAGACAAGTA CCAGGTGTTT 
GATTCGGCCC CAAGGGGCCT GCTTCGAGTG GAGGAGCTGG AGGACCAGGG TCAAACACTG 
GCTAATGTGT TCATCCTGCG GCTGCTGGAA AATGCAGATG ACCGAGAGGC CACCTATATG 
CTGAAGGCAT CCTCCCAGAG CGAGATGAAG CGCTGGATGA CCTCACTGGC CCCCAACAGG 
AGGACCAAGT TTGTATCCTT CACATCTCGG CTGTTGGACT GTCCCCAGGT CCAGTGTGTG 
CACCCGTATG TGGCCCAGCA GCCTGATGAA CTGACGCTGG AACTGGCAGA TATCCTGAAC 
ATCCTGGAGA AGACAGAGGA TGGGTGGATC TTTGGTGAGC GGCTGCATGA CCAGGAGAGA 
GGCTGGTTCC CCAGTTCCAT GACAGAGGAG ATCCTGAACC CCAAGATCCG CTCCCAGAAC 
CTCAAGGAAT GTTTCCGGGT ACATAAGATG GAAGACCCTC AGCGCAGCCA GAATAAGGAC 
CGCAGGAAGC TGGGCAGCCG GAATCGTCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104784.1
CDS	257..2389
Translation	METKNSEDWGKPQRKSESSSRKSNHGPAEMRPALPPENREAPETGEETQNEEPRRLIPIQRHSLFNRAVRHRHKARSTSERRASDQADLPKMGKSVNERSAFNLPQGRLPPWRTPAQRDTGAQEASESSSTPGNGTTPEECPALTDSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIQGNSDGSQVGEDAGEEEEEEEEGEEEELASPPERRALPQICLLSNPHSRFNLWQDLPEIQSSGVLDILQPEEIRLQEAMFELVTSEASYYKSLNLLVSHFMENERLKKILHPSEAHILFSNVLDVMAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCKGLPFSSFLILPFQRITRLKLLVQNILKRVEERSEREGTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILEKTEDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMEDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	NM_001111314.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuronal guanine nucleotide exchange factor (Ngef), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001111314.1

ORF Insert Sequence:

ATGGAGACCA AAAACTCTGA AGACTGGGGG AAACCTCAGA GGAAATCAGA GAGCAGCTCA 
CGGAAAAGCA ATCATGGCCC AGCTGAGATG AGGCCTGCGT TACCTCCGGA GAACAGGGAG 
GCACCTGAGA CTGGCGAGGA AACTCAAAAC GAAGAACCCC GCCGCCTCAT CCCAATCCAG 
AGGCATTCCC TCTTCAATCG TGCTGTGAGA CACAGGCACA AGGCCAGGAG CACGTCTGAG 
CGGAGAGCCA GTGACCAAGC AGATTTGCCA AAAATGGGGA AATCTGTAAA TGAACGGTCG 
GCCTTCAATC TGCCCCAGGG GAGATTGCCT CCCTGGAGAA CACCAGCCCA GAGGGACACA 
GGAGCGCAGG AAGCCAGCGA GTCATCCTCC ACCCCTGGAA ATGGAACCAC ACCCGAGGAG 
TGCCCAGCCC TCACCGACAG CCCCACCACT CTCACGGAGG CCCTGCAGAT GATCCATCCC 
ATTCCCGCCG ACTCCTGGAG AAACCTCATT GAACAAATAG GGCTCCTGTA TCAAGAGTAT 
AGAGACAAAT CGACTCTCCA AGAAATTGAA ACACGGAGGC AGCAGGATGC AGAAATCCAA 
GGCAACTCCG ATGGGTCCCA GGTTGGGGAA GACGCTGGAG AGGAGGAGGA GGAGGAGGAG 
GAGGGAGAGG AGGAGGAGCT GGCCAGCCCT CCTGAGAGGA GAGCTCTGCC TCAGATCTGC 
CTGCTCAGCA ACCCCCACTC CAGGTTCAAC CTCTGGCAAG ACCTTCCTGA GATCCAGAGC 
AGTGGCGTGC TGGACATTCT CCAGCCGGAG GAGATCAGGC TGCAGGAGGC CATGTTTGAG 
TTGGTTACCT CTGAGGCCTC CTACTATAAG AGCCTGAACC TGCTGGTGTC GCACTTCATG 
GAGAACGAGC GTCTGAAGAA GATCCTGCAT CCATCTGAGG CCCACATCCT CTTTTCCAAT 
GTCCTGGATG TCATGGCTGT CAGTGAGCGG TTTTTGCTGG AGCTAGAGCA CCGCATGGAG 
GAGAACATTG TTATCTCGGA TGTGTGCGAC ATCGTGTACC GTTACGCAGC TGATCACTTC 
TCGGTCTATA TCACTTACGT CAGTAACCAG ACCTACCAGG AAAGGACATA CAAGCAGCTC 
CTACAGGAGA AGGCCGCTTT CCGGGAACTG ATCGCACAGT TGGAGCTGGA CCCCAAATGC 
AAGGGCCTGC CTTTCTCCTC CTTCCTCATC TTGCCTTTCC AGAGGATCAC GAGACTCAAG 
CTGCTGGTCC AGAATATCCT GAAGAGAGTG GAGGAGAGGT CTGAACGTGA AGGCACCGCC 
TTGGATGCCC ACAAGGAGCT AGAAATGGTG GTAAAGGCAT GCAATGAGGG TGTCCGGAAG 
ATGAGCCGCA CAGAACAGAT GATCAGCATT CAGAAGAAGA TGGAGTTCAA GATCAAGTCG 
GTACCCATCA TCTCACACTC CCGGTGGCTG CTGAAGCAGG GTGAGCTGCA GCAGATGTCC 
GGCCCCAAGA CCTCCCGCAC CCTGCGGACC AAGAAGCTCT TCAGAGAAAT TTACCTCTTC 
CTCTTCAATG ACCTGCTGGT GATCTGCCGG CAGATCCCTG GAGACAAGTA CCAGGTGTTT 
GATTCGGCCC CAAGGGGCCT GCTTCGAGTG GAGGAGCTGG AGGACCAGGG TCAAACACTG 
GCTAATGTGT TCATCCTGCG GCTGCTGGAA AATGCAGATG ACCGAGAGGC CACCTATATG 
CTGAAGGCAT CCTCCCAGAG CGAGATGAAG CGCTGGATGA CCTCACTGGC CCCCAACAGG 
AGGACCAAGT TTGTATCCTT CACATCTCGG CTGTTGGACT GTCCCCAGGT CCAGTGTGTG 
CACCCGTATG TGGCCCAGCA GCCTGATGAA CTGACGCTGG AACTGGCAGA TATCCTGAAC 
ATCCTGGAGA AGACAGAGGA TGGGTGGATC TTTGGTGAGC GGCTGCATGA CCAGGAGAGA 
GGCTGGTTCC CCAGTTCCAT GACAGAGGAG ATCCTGAACC CCAAGATCCG CTCCCAGAAC 
CTCAAGGAAT GTTTCCGGGT ACATAAGATG GAAGACCCTC AGCGCAGCCA GAATAAGGAC 
CGCAGGAAGC TGGGCAGCCG GAATCGTCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ngef ( NM_019867.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_063920.2 Mus musculus neuronal guanine nucleotide exchange factor (Ngef), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu21224
Clone ID Related Accession (Same CDS sequence)	NM_019867.2
Accession Version	NM_019867.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Mus musculus(house mouse)
Product	ephexin-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ149200.1, AK138433.1, AJ238898.1 and AI847102.1. On Dec 13, 2007 this sequence version replaced NM_019867.1. Transcript Variant: This variant (2) contains a different segment for its 5' UTR and 5' coding region, and lacks other coding region exons, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct N-terminus when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ238898.1, AY038025.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCTGC TGGCTGCAGC CTTCAGCGCC GCCTGCGCCG TGGACCACGA CAGCTCCACC 
TCGGAGAGCG ACACGCGCGA CTCGGCGGCG GGACACCTGC CGGGCAGCGA GTCATCCTCC 
ACCCCTGGAA ATGGAACCAC ACCCGAGGAG TGCCCAGCCC TCACCGACAG CCCCACCACT 
CTCACGGAGG CCCTGCAGAT GATCCATCCC ATTCCCGCCG ACTCCTGGAG AAACCTCATT 
GAACAAATAG GGCTCCTGTA TCAAGAGTAT AGAGACAAAT CGACTCTCCA AGAAATTGAA 
ACACGGAGGC AGCAGGATGC AGAAATCCAA GGCAACTCCG ATGGGTCCCA GGTTGGGGAA 
GACGCTGGAG AGGAGGAGGA GGAGGAGGAG GAGGGAGAGG AGGAGGAGCT GGCCAGCCCT 
CCTGAGAGGA GAGCTCTGCC TCAGATCTGC CTGCTCAGCA ACCCCCACTC CAGGTTCAAC 
CTCTGGCAAG ACCTTCCTGA GATCCAGAGC AGTGGCGTGC TGGACATTCT CCAGCCGGAG 
GAGATCAGGC TGCAGGAGGC CATGTTTGAG TTGGTTACCT CTGAGGCCTC CTACTATAAG 
AGCCTGAACC TGCTGGTGTC GCACTTCATG GAGAACGAGC GTCTGAAGAA GATCCTGCAT 
CCATCTGAGG CCCACATCCT CTTTTCCAAT GTCCTGGATG TCATGGCTGT CAGTGAGCGG 
TTTTTGCTGG AGCTAGAGCA CCGCATGGAG GAGAACATTG TTATCTCGGA TGTGTGCGAC 
ATCGTGTACC GTTACGCAGC TGATCACTTC TCGGTCTATA TCACTTACGT CAGTAACCAG 
ACCTACCAGG AAAGGACATA CAAGCAGCTC CTACAGGAGA AGGCCGCTTT CCGGGAACTG 
ATCGCACAGT TGGAGCTGGA CCCCAAATGC AAGGGCCTGC CTTTCTCCTC CTTCCTCATC 
TTGCCTTTCC AGAGGATCAC GAGACTCAAG CTGCTGGTCC AGAATATCCT GAAGAGAGTG 
GAGGAGAGGT CTGAACGTGA AGGCACCGCC TTGGATGCCC ACAAGGAGCT AGAAATGGTG 
GTAAAGGCAT GCAATGAGGG TGTCCGGAAG ATGAGCCGCA CAGAACAGAT GATCAGCATT 
CAGAAGAAGA TGGAGTTCAA GATCAAGTCG GTACCCATCA TCTCACACTC CCGGTGGCTG 
CTGAAGCAGG GTGAGCTGCA GCAGATGTCC GGCCCCAAGA CCTCCCGCAC CCTGCGGACC 
AAGAAGCTCT TCAGAGAAAT TTACCTCTTC CTCTTCAATG ACCTGCTGGT GATCTGCCGG 
CAGATCCCTG GAGACAAGTA CCAGGTGTTT GATTCGGCCC CAAGGGGCCT GCTTCGAGTG 
GAGGAGCTGG AGGACCAGGG TCAAACACTG GCTAATGTGT TCATCCTGCG GCTGCTGGAA 
AATGCAGATG ACCGAGAGGC CACCTATATG CTGAAGGCAT CCTCCCAGAG CGAGATGAAG 
CGCTGGATGA CCTCACTGGC CCCCAACAGG AGGACCAAGT TTGTATCCTT CACATCTCGG 
CTGTTGGACT GTCCCCAGGT CCAGTGTGTG CACCCGTATG TGGCCCAGCA GCCTGATGAA 
CTGACGCTGG AACTGGCAGA TATCCTGAAC ATCCTGGAGA AGACAGAGGA TGGGTGGATC 
TTTGGTGAGC GGCTGCATGA CCAGGAGAGA GGCTGGTTCC CCAGTTCCAT GACAGAGGAG 
ATCCTGAACC CCAAGATCCG CTCCCAGAAC CTCAAGGAAT GTTTCCGGGT ACATAAGATG 
GAAGACCCTC AGCGCAGCCA GAATAAGGAC CGCAGGAAGC TGGGCAGCCG GAATCGTCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_063920.2
CDS	164..2026
Translation	MELLAAAFSAACAVDHDSSTSESDTRDSAAGHLPGSESSSTPGNGTTPEECPALTDSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIQGNSDGSQVGEDAGEEEEEEEEGEEEELASPPERRALPQICLLSNPHSRFNLWQDLPEIQSSGVLDILQPEEIRLQEAMFELVTSEASYYKSLNLLVSHFMENERLKKILHPSEAHILFSNVLDVMAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCKGLPFSSFLILPFQRITRLKLLVQNILKRVEERSEREGTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILEKTEDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMEDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	NM_019867.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuronal guanine nucleotide exchange factor (Ngef), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019867.2

ORF Insert Sequence:

ATGGAGCTGC TGGCTGCAGC CTTCAGCGCC GCCTGCGCCG TGGACCACGA CAGCTCCACC 
TCGGAGAGCG ACACGCGCGA CTCGGCGGCG GGACACCTGC CGGGCAGCGA GTCATCCTCC 
ACCCCTGGAA ATGGAACCAC ACCCGAGGAG TGCCCAGCCC TCACCGACAG CCCCACCACT 
CTCACGGAGG CCCTGCAGAT GATCCATCCC ATTCCCGCCG ACTCCTGGAG AAACCTCATT 
GAACAAATAG GGCTCCTGTA TCAAGAGTAT AGAGACAAAT CGACTCTCCA AGAAATTGAA 
ACACGGAGGC AGCAGGATGC AGAAATCCAA GGCAACTCCG ATGGGTCCCA GGTTGGGGAA 
GACGCTGGAG AGGAGGAGGA GGAGGAGGAG GAGGGAGAGG AGGAGGAGCT GGCCAGCCCT 
CCTGAGAGGA GAGCTCTGCC TCAGATCTGC CTGCTCAGCA ACCCCCACTC CAGGTTCAAC 
CTCTGGCAAG ACCTTCCTGA GATCCAGAGC AGTGGCGTGC TGGACATTCT CCAGCCGGAG 
GAGATCAGGC TGCAGGAGGC CATGTTTGAG TTGGTTACCT CTGAGGCCTC CTACTATAAG 
AGCCTGAACC TGCTGGTGTC GCACTTCATG GAGAACGAGC GTCTGAAGAA GATCCTGCAT 
CCATCTGAGG CCCACATCCT CTTTTCCAAT GTCCTGGATG TCATGGCTGT CAGTGAGCGG 
TTTTTGCTGG AGCTAGAGCA CCGCATGGAG GAGAACATTG TTATCTCGGA TGTGTGCGAC 
ATCGTGTACC GTTACGCAGC TGATCACTTC TCGGTCTATA TCACTTACGT CAGTAACCAG 
ACCTACCAGG AAAGGACATA CAAGCAGCTC CTACAGGAGA AGGCCGCTTT CCGGGAACTG 
ATCGCACAGT TGGAGCTGGA CCCCAAATGC AAGGGCCTGC CTTTCTCCTC CTTCCTCATC 
TTGCCTTTCC AGAGGATCAC GAGACTCAAG CTGCTGGTCC AGAATATCCT GAAGAGAGTG 
GAGGAGAGGT CTGAACGTGA AGGCACCGCC TTGGATGCCC ACAAGGAGCT AGAAATGGTG 
GTAAAGGCAT GCAATGAGGG TGTCCGGAAG ATGAGCCGCA CAGAACAGAT GATCAGCATT 
CAGAAGAAGA TGGAGTTCAA GATCAAGTCG GTACCCATCA TCTCACACTC CCGGTGGCTG 
CTGAAGCAGG GTGAGCTGCA GCAGATGTCC GGCCCCAAGA CCTCCCGCAC CCTGCGGACC 
AAGAAGCTCT TCAGAGAAAT TTACCTCTTC CTCTTCAATG ACCTGCTGGT GATCTGCCGG 
CAGATCCCTG GAGACAAGTA CCAGGTGTTT GATTCGGCCC CAAGGGGCCT GCTTCGAGTG 
GAGGAGCTGG AGGACCAGGG TCAAACACTG GCTAATGTGT TCATCCTGCG GCTGCTGGAA 
AATGCAGATG ACCGAGAGGC CACCTATATG CTGAAGGCAT CCTCCCAGAG CGAGATGAAG 
CGCTGGATGA CCTCACTGGC CCCCAACAGG AGGACCAAGT TTGTATCCTT CACATCTCGG 
CTGTTGGACT GTCCCCAGGT CCAGTGTGTG CACCCGTATG TGGCCCAGCA GCCTGATGAA 
CTGACGCTGG AACTGGCAGA TATCCTGAAC ATCCTGGAGA AGACAGAGGA TGGGTGGATC 
TTTGGTGAGC GGCTGCATGA CCAGGAGAGA GGCTGGTTCC CCAGTTCCAT GACAGAGGAG 
ATCCTGAACC CCAAGATCCG CTCCCAGAAC CTCAAGGAAT GTTTCCGGGT ACATAAGATG 
GAAGACCCTC AGCGCAGCCA GAATAAGGAC CGCAGGAAGC TGGGCAGCCG GAATCGTCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increased EphA4-ephexin1 signaling in the medial prefrontal cortex plays a role in depression-like phenotype.
Scientific reports7(1)7133(2017 Aug)
Zhang JC,Yao W,Qu Y,Nakamura M,Dong C,Yang C,Ren Q,Ma M,Han M,Shirayama Y,Hayashi-Takagi A,Hashimoto K

FoxP1 orchestration of ASD-relevant signaling pathways in the striatum.
Genes & development29(20)2081-96(2015 Oct)
Araujo DJ,Anderson AG,Berto S,Runnels W,Harper M,Ammanuel S,Rieger MA,Huang HC,Rajkovich K,Loerwald KW,Dekker JD,Tucker HO,Dougherty JD,Gibson JR,Konopka G

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Ngef cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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