NGEF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NGEF
Entrez Gene ID	25791
Full Name	neuronal guanine nucleotide exchange factor
Synonyms	ARHGEF27,EPHEXIN
General protein information	Preferred Names neuronal guanine nucleotide exchange factor Names ephexin-1 eph-interacting exchange protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.1
Disorder MIM:	605991

mRNA and Protein(s)

mRNA	Protein	Name
XM_011510924.1	XP_011509226.1	ephexin-1 isoform X2
XM_011510923.2	XP_011509225.1	ephexin-1 isoform X1
NM_019850.2	NP_062824.2	ephexin-1 isoform 1
NM_001114090.2	NP_001107562.1	ephexin-1 isoform 2
XM_011510925.1	XP_011509227.1	ephexin-1 isoform X2
NM_019850.3	NP_062824.2	ephexin-1 isoform 1
NM_001114090.1	NP_001107562.1	ephexin-1 isoform 2
XM_011510923.3	XP_011509225.1	ephexin-1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NGEF	XP_005635921.1
Danio rerio (zebrafish)	LOC569090	XP_697546.3
Xenopus tropicalis (tropical clawed frog)	ngef	XP_004917852.1
Bos taurus (cattle)	NGEF	NP_001070394.1
Rattus norvegicus (Norway rat)	Ngef	NP_001129713.1
Pan troglodytes (chimpanzee)	NGEF	XP_516157.3
Homo sapiens (human)	NGEF	NP_062824.2
	LOC713917	XP_001114604.1
Mus musculus (house mouse)	Ngef	NP_001104784.1
Gallus gallus (chicken)	NGEF	NP_001010841.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007452	XP_308411.4

Gene Ontology
Bibliography

Related articles in PubMed

A Common Variant of NGEF Is Associated with Abdominal Visceral Fat in Korean Men.
Kim HJ, Park JH, Lee S, Son HY, Hwang J, Chae J, Yun JM, Kwon H, Kim JI, Cho B
PloS one10(9)e0137564(2015)

Biological insights from 108 schizophrenia-associated genetic loci.

Nature511(7510)421-7(2014 Jul)

Variants in the 1q21 risk region are associated with a visual endophenotype of autism and schizophrenia.
Goodbourn PT, Bosten JM, Bargary G, Hogg RE, Lawrance-Owen AJ, Mollon JD
Genes, brain, and behavior13(2)144-51(2014 Feb)

Genome-wide association analysis identifies 13 new risk loci for schizophrenia.
Ripke S, O'Dushlaine C, Chambert K, Moran JL, K?hler AK, Akterin S, Bergen SE, Collins AL, Crowley JJ, Fromer M, Kim Y, Lee SH, Magnusson PK, Sanchez N, Stahl EA, Williams S, Wray NR, Xia K, Bettella F, Borglum AD, Bulik-Sullivan BK, Cormican P, Craddock N, de Leeuw C, Durmishi N, Gill M, Golimbet V, Hamshere ML, Holmans P, Hougaard DM, Kendler KS, Lin K, Morris DW, Mors O, Mortensen PB, Neale BM, O'Neill FA, Owen MJ, Milovancevic MP, Posthuma D, Powell J, Richards AL, Riley BP, Ruderfer D, Rujescu D, Sigurdsson E, Silagadze T, Smit AB, Stefansson H, Steinberg S, Suvisaari J, Tosato S, Verhage M, Walters JT, , Levinson DF, Gejman PV, Kendler KS, Laurent C, Mowry BJ, O'Donovan MC, Owen MJ, Pulver AE, Riley BP, Schwab SG, Wildenauer DB, Dudbridge F, Holmans P, Shi J, Albus M, Alexander M, Campion D, Cohen D, Dikeos D, Duan J, Eichhammer P, Godard S, Hansen M, Lerer FB, Liang KY, Maier W, Mallet J, Nertney DA, Nestadt G, Norton N, O'Neill FA, Papadimitriou GN, Ribble R, Sanders AR, Silverman JM, Walsh D, Williams NM, Wormley B, , Arranz MJ, Bakker S, Bender S, Bramon E, Collier D, Crespo-Facorro B, Hall J, Iyegbe C, Jablensky A, Kahn RS, Kalaydjieva L, Lawrie S, Lewis CM, Lin K, Linszen DH, Mata I, McIntosh A, Murray RM, Ophoff RA, Powell J, Rujescu D, Van Os J, Walshe M, Weisbrod M, Wiersma D, , Donnelly P, Barroso I, Blackwell JM, Bramon E, Brown MA, Casas JP, Corvin AP, Deloukas P, Duncanson A, Jankowski J, Markus HS, Mathew CG, Palmer CN, Plomin R, Rautanen A, Sawcer SJ, Trembath RC, Viswanathan AC, Wood NW, Spencer CC, Band G, Bellenguez C, Freeman C, Hellenthal G, Giannoulatou E, Pirinen M, Pearson RD, Strange A, Su Z, Vukcevic D, Donnelly P, Langford C, Hunt SE, Edkins S, Gwilliam R, Blackburn H, Bumpstead SJ, Dronov S, Gillman M, Gray E, Hammond N, Jayakumar A, McCann OT, Liddle J, Potter SC, Ravindrarajah R, Ricketts M, Tashakkori-Ghanbaria A, Waller MJ, Weston P, Widaa S, Whittaker P, Barroso I, Deloukas P, Mathew CG, Blackwell JM, Brown MA, Corvin AP, McCarthy MI, Spencer CC, Bramon E, Corvin AP, O'Donovan MC, Stefansson K, Scolnick E, Purcell S, McCarroll SA, Sklar P, Hultman CM, Sullivan PF
Nature genetics45(10)1150-9(2013 Oct)

Identification and analysis of intermediate size noncoding RNAs in the human fetal brain.
Yan D, He D, He S, Chen X, Fan Z, Chen R
PloS one6(7)e21652(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A visceral-fat-associated SNP, rs11678490 of NGEF, was found in Korean men.
Title: A Common Variant of NGEF Is Associated with Abdominal Visceral Fat in Korean Men.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association. (HuGE Navigator)
Title: Mutation of ARHGAP9 in patients with coronary spastic angina.

FGFR-mediated phosphorylation of ephexin1 enhances the guanine nucleotide exchange activity toward RhoA without affecting the activity to Rac1 or Cdc42.
Title: Regulation of ephexin1, a guanine nucleotide exchange factor of Rho family GTPases, by fibroblast growth factor receptor-mediated tyrosine phosphorylation.

The following NGEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16623	NM_019850.3 Latest version!	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu16462	NM_001114090.2 Latest version!	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu66488	XM_011510924.1 Latest version!	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu66488	XM_011510925.1 Latest version!	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu16623	XM_011510923.3 Latest version!	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu16623	NM_019850.2	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu16462	NM_001114090.1	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu16623	XM_011510923.2	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NGEF ( NM_019850.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_062824.2 Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16623
Clone ID Related Accession (Same CDS sequence)	XM_011510923.2 , NM_019850.2 , NM_019850.3 , XM_011510923.3
Accession Version	NM_019850.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	ephexin-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC106876.3, DB242442.1, DC388448.1, BC073962.1, BX431262.1, BC031573.1 and AC016692.9. On Nov 22, 2018 this sequence version replaced NM_019850.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031573.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264051.8/ ENSP00000264051.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGACCA GGGAATCTGA AGATTTGGAA AAGACCCGGA GGAAATCAGC AAGTGATCAA 
TGGAACACTG ATAATGAACC AGCCAAGGTG AAACCTGAGT TACTCCCAGA AAAAGAGGAG 
ACTTCTCAAG CTGACCAGGA TATCCAAGAC AAAGAGCCTC ATTGCCACAT CCCAATTAAG 
AGAAATTCCA TCTTCAATCG CTCCATAAGA CGCAAAAGCA AAGCCAAGGC CAGAGACAAC 
CCCGAACGGA ACGCCAGCTG CCTGGCAGAT TCACAGGACA ATGGAAAATC TGTAAATGAG 
CCCCTGACCT TGAATATCCC CTGGAGCAGA ATGCCTCCTT GCAGAACAGC AATGCAGACA 
GACCCAGGAG CCCAGGAAAT GAGTGAGTCG TCCTCCACCC CGGGAAATGG GGCCACGCCC 
GAGGAGTGGC CGGCCCTGGC CGACAGCCCC ACCACGCTCA CCGAGGCCCT GCGGATGATC 
CACCCCATTC CCGCCGACTC CTGGAGAAAC CTCATTGAAC AAATAGGGCT CCTGTATCAG 
GAATACCGAG ATAAATCGAC TCTCCAAGAA ATCGAAACCA GGAGGCAACA GGATGCAGAA 
ATAGAAGACA ATACCAATGG GTCCCCGGCC AGTGAGGACA CCCCGGAGGA GGAAGAAGAA 
GAGGAGGAGG AGGAGGAGCC GGCCAGCCCA CCAGAGAGGA AGACTCTGCC CCAGATCTGC 
CTGCTCAGTA ACCCCCACTC AAGGTTCAAC CTCTGGCAGG ATCTTCCCGA GATCCGGAGC 
AGCGGGGTGC TTGAGATCCT ACAGCCCGAG GAGATTAAGC TGCAGGAGGC CATGTTCGAG 
CTGGTCACTT CCGAGGCGTC CTACTACAAG AGTCTGAACC TGCTCGTGTC CCACTTCATG 
GAGAACGAGC GGATAAGGAA GATCCTGCAC CCGTCCGAGG CGCACATCCT CTTCTCCAAC 
GTCCTGGACG TGCTGGCTGT CAGTGAGCGG TTCCTCCTGG AGCTGGAGCA CCGGATGGAG 
GAGAACATCG TCATCTCTGA CGTGTGTGAC ATTGTGTACC GTTATGCGGC CGACCACTTC 
TCTGTCTACA TCACCTACGT CAGCAATCAG ACCTACCAGG AGCGGACCTA TAAGCAGCTG 
CTCCAGGAGA AGGCAGCTTT CCGGGAGCTG ATCGCGCAGC TAGAGCTCGA CCCCAAGTGC 
AGGGGGCTGC CCTTCTCCTC CTTCCTCATC CTGCCTTTCC AGAGGATCAC ACGCCTCAAG 
CTGTTGGTCC AGAACATCCT GAAGAGGGTA GAAGAGAGGT CTGAGCGGGA GTGCACTGCT 
TTGGATGCTC ACAAGGAGCT GGAAATGGTG GTGAAGGCAT GCAACGAGGG CGTCAGGAAA 
ATGAGCCGCA CGGAACAGAT GATCAGCATT CAGAAGAAGA TGGAGTTCAA GATCAAGTCG 
GTGCCCATCA TCTCCCACTC CCGCTGGCTG CTGAAGCAGG GTGAGCTGCA GCAGATGTCA 
GGCCCCAAGA CCTCCCGGAC CCTGAGGACC AAGAAGCTCT TCCACGAAAT TTACCTCTTC 
CTGTTCAACG ACCTGCTGGT GATCTGCCGG CAGATTCCAG GAGACAAGTA CCAGGTATTT 
GACTCAGCTC CGCGGGGACT GCTGCGTGTG GAGGAGCTGG AGGACCAGGG CCAGACGCTG 
GCCAACGTGT TCATCCTGCG GCTGCTGGAG AACGCAGATG ACCGGGAGGC CACCTACATG 
CTAAAGGCGT CCTCTCAGAG TGAGATGAAG CGTTGGATGA CCTCACTGGC CCCCAACAGG 
AGGACCAAGT TTGTTTCGTT CACATCCCGG CTGCTGGACT GCCCCCAGGT CCAGTGCGTG 
CACCCATACG TGGCTCAGCA GCCAGACGAG CTGACGCTGG AGCTCGCCGA CATCCTCAAC 
ATCCTGGACA AGACTGACGA CGGGTGGATC TTTGGCGAGC GTCTGCACGA CCAGGAGAGA 
GGCTGGTTCC CCAGCTCCAT GACTGAGGAG ATCTTGAATC CCAAGATCCG GTCCCAGAAC 
CTCAAGGAAT GTTTCCGTGT CCACAAGATG GATGACCCTC AGCGCAGCCA GAACAAGGAC 
CGCAGGAAGC TGGGCAGCCG GAATCGGCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062824.2
CDS	264..2396
Translation	METRESEDLEKTRRKSASDQWNTDNEPAKVKPELLPEKEETSQADQDIQDKEPHCHIPIKRNSIFNRSIRRKSKAKARDNPERNASCLADSQDNGKSVNEPLTLNIPWSRMPPCRTAMQTDPGAQEMSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	NM_019850.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019850.3

ORF Insert Sequence:

ATGGAGACCA GGGAATCTGA AGATTTGGAA AAGACCCGGA GGAAATCAGC AAGTGATCAA 
TGGAACACTG ATAATGAACC AGCCAAGGTG AAACCTGAGT TACTCCCAGA AAAAGAGGAG 
ACTTCTCAAG CTGACCAGGA TATCCAAGAC AAAGAGCCTC ATTGCCACAT CCCAATTAAG 
AGAAATTCCA TCTTCAATCG CTCCATAAGA CGCAAAAGCA AAGCCAAGGC CAGAGACAAC 
CCCGAACGGA ACGCCAGCTG CCTGGCAGAT TCACAGGACA ATGGAAAATC TGTAAATGAG 
CCCCTGACCT TGAATATCCC CTGGAGCAGA ATGCCTCCTT GCAGAACAGC AATGCAGACA 
GACCCAGGAG CCCAGGAAAT GAGTGAGTCG TCCTCCACCC CGGGAAATGG GGCCACGCCC 
GAGGAGTGGC CGGCCCTGGC CGACAGCCCC ACCACGCTCA CCGAGGCCCT GCGGATGATC 
CACCCCATTC CCGCCGACTC CTGGAGAAAC CTCATTGAAC AAATAGGGCT CCTGTATCAG 
GAATACCGAG ATAAATCGAC TCTCCAAGAA ATCGAAACCA GGAGGCAACA GGATGCAGAA 
ATAGAAGACA ATACCAATGG GTCCCCGGCC AGTGAGGACA CCCCGGAGGA GGAAGAAGAA 
GAGGAGGAGG AGGAGGAGCC GGCCAGCCCA CCAGAGAGGA AGACTCTGCC CCAGATCTGC 
CTGCTCAGTA ACCCCCACTC AAGGTTCAAC CTCTGGCAGG ATCTTCCCGA GATCCGGAGC 
AGCGGGGTGC TTGAGATCCT ACAGCCCGAG GAGATTAAGC TGCAGGAGGC CATGTTCGAG 
CTGGTCACTT CCGAGGCGTC CTACTACAAG AGTCTGAACC TGCTCGTGTC CCACTTCATG 
GAGAACGAGC GGATAAGGAA GATCCTGCAC CCGTCCGAGG CGCACATCCT CTTCTCCAAC 
GTCCTGGACG TGCTGGCTGT CAGTGAGCGG TTCCTCCTGG AGCTGGAGCA CCGGATGGAG 
GAGAACATCG TCATCTCTGA CGTGTGTGAC ATTGTGTACC GTTATGCGGC CGACCACTTC 
TCTGTCTACA TCACCTACGT CAGCAATCAG ACCTACCAGG AGCGGACCTA TAAGCAGCTG 
CTCCAGGAGA AGGCAGCTTT CCGGGAGCTG ATCGCGCAGC TAGAGCTCGA CCCCAAGTGC 
AGGGGGCTGC CCTTCTCCTC CTTCCTCATC CTGCCTTTCC AGAGGATCAC ACGCCTCAAG 
CTGTTGGTCC AGAACATCCT GAAGAGGGTA GAAGAGAGGT CTGAGCGGGA GTGCACTGCT 
TTGGATGCTC ACAAGGAGCT GGAAATGGTG GTGAAGGCAT GCAACGAGGG CGTCAGGAAA 
ATGAGCCGCA CGGAACAGAT GATCAGCATT CAGAAGAAGA TGGAGTTCAA GATCAAGTCG 
GTGCCCATCA TCTCCCACTC CCGCTGGCTG CTGAAGCAGG GTGAGCTGCA GCAGATGTCA 
GGCCCCAAGA CCTCCCGGAC CCTGAGGACC AAGAAGCTCT TCCACGAAAT TTACCTCTTC 
CTGTTCAACG ACCTGCTGGT GATCTGCCGG CAGATTCCAG GAGACAAGTA CCAGGTATTT 
GACTCAGCTC CGCGGGGACT GCTGCGTGTG GAGGAGCTGG AGGACCAGGG CCAGACGCTG 
GCCAACGTGT TCATCCTGCG GCTGCTGGAG AACGCAGATG ACCGGGAGGC CACCTACATG 
CTAAAGGCGT CCTCTCAGAG TGAGATGAAG CGTTGGATGA CCTCACTGGC CCCCAACAGG 
AGGACCAAGT TTGTTTCGTT CACATCCCGG CTGCTGGACT GCCCCCAGGT CCAGTGCGTG 
CACCCATACG TGGCTCAGCA GCCAGACGAG CTGACGCTGG AGCTCGCCGA CATCCTCAAC 
ATCCTGGACA AGACTGACGA CGGGTGGATC TTTGGCGAGC GTCTGCACGA CCAGGAGAGA 
GGCTGGTTCC CCAGCTCCAT GACTGAGGAG ATCTTGAATC CCAAGATCCG GTCCCAGAAC 
CTCAAGGAAT GTTTCCGTGT CCACAAGATG GATGACCCTC AGCGCAGCCA GAACAAGGAC 
CGCAGGAAGC TGGGCAGCCG GAATCGGCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGEF ( NM_001114090.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107562.1 Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16462
Clone ID Related Accession (Same CDS sequence)	NM_001114090.2 , NM_001114090.1
Accession Version	NM_001114090.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	ephexin-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC073962.1, BX431262.1, BC031573.1 and AC016692.9. On May 31, 2019 this sequence version replaced NM_001114090.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297390.1, SRR1660805.111997.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGC TGGCCGCTGC CTTCAGCGCC GCCTGCGCCG TGGACCACGA CAGTTCCACC 
TCGGAAAGCG ACGCGCGCGA CTCGGCGGCG GGACACCTGC CCGGCAGTGA GTCGTCCTCC 
ACCCCGGGAA ATGGGGCCAC GCCCGAGGAG TGGCCGGCCC TGGCCGACAG CCCCACCACG 
CTCACCGAGG CCCTGCGGAT GATCCACCCC ATTCCCGCCG ACTCCTGGAG AAACCTCATT 
GAACAAATAG GGCTCCTGTA TCAGGAATAC CGAGATAAAT CGACTCTCCA AGAAATCGAA 
ACCAGGAGGC AACAGGATGC AGAAATAGAA GACAATACCA ATGGGTCCCC GGCCAGTGAG 
GACACCCCGG AGGAGGAAGA AGAAGAGGAG GAGGAGGAGG AGCCGGCCAG CCCACCAGAG 
AGGAAGACTC TGCCCCAGAT CTGCCTGCTC AGTAACCCCC ACTCAAGGTT CAACCTCTGG 
CAGGATCTTC CCGAGATCCG GAGCAGCGGG GTGCTTGAGA TCCTACAGCC CGAGGAGATT 
AAGCTGCAGG AGGCCATGTT CGAGCTGGTC ACTTCCGAGG CGTCCTACTA CAAGAGTCTG 
AACCTGCTCG TGTCCCACTT CATGGAGAAC GAGCGGATAA GGAAGATCCT GCACCCGTCC 
GAGGCGCACA TCCTCTTCTC CAACGTCCTG GACGTGCTGG CTGTCAGTGA GCGGTTCCTC 
CTGGAGCTGG AGCACCGGAT GGAGGAGAAC ATCGTCATCT CTGACGTGTG TGACATTGTG 
TACCGTTATG CGGCCGACCA CTTCTCTGTC TACATCACCT ACGTCAGCAA TCAGACCTAC 
CAGGAGCGGA CCTATAAGCA GCTGCTCCAG GAGAAGGCAG CTTTCCGGGA GCTGATCGCG 
CAGCTAGAGC TCGACCCCAA GTGCAGGGGG CTGCCCTTCT CCTCCTTCCT CATCCTGCCT 
TTCCAGAGGA TCACACGCCT CAAGCTGTTG GTCCAGAACA TCCTGAAGAG GGTAGAAGAG 
AGGTCTGAGC GGGAGTGCAC TGCTTTGGAT GCTCACAAGG AGCTGGAAAT GGTGGTGAAG 
GCATGCAACG AGGGCGTCAG GAAAATGAGC CGCACGGAAC AGATGATCAG CATTCAGAAG 
AAGATGGAGT TCAAGATCAA GTCGGTGCCC ATCATCTCCC ACTCCCGCTG GCTGCTGAAG 
CAGGGTGAGC TGCAGCAGAT GTCAGGCCCC AAGACCTCCC GGACCCTGAG GACCAAGAAG 
CTCTTCCACG AAATTTACCT CTTCCTGTTC AACGACCTGC TGGTGATCTG CCGGCAGATT 
CCAGGAGACA AGTACCAGGT ATTTGACTCA GCTCCGCGGG GACTGCTGCG TGTGGAGGAG 
CTGGAGGACC AGGGCCAGAC GCTGGCCAAC GTGTTCATCC TGCGGCTGCT GGAGAACGCA 
GATGACCGGG AGGCCACCTA CATGCTAAAG GCGTCCTCTC AGAGTGAGAT GAAGCGTTGG 
ATGACCTCAC TGGCCCCCAA CAGGAGGACC AAGTTTGTTT CGTTCACATC CCGGCTGCTG 
GACTGCCCCC AGGTCCAGTG CGTGCACCCA TACGTGGCTC AGCAGCCAGA CGAGCTGACG 
CTGGAGCTCG CCGACATCCT CAACATCCTG GACAAGACTG ACGACGGGTG GATCTTTGGC 
GAGCGTCTGC ACGACCAGGA GAGAGGCTGG TTCCCCAGCT CCATGACTGA GGAGATCTTG 
AATCCCAAGA TCCGGTCCCA GAACCTCAAG GAATGTTTCC GTGTCCACAA GATGGATGAC 
CCTCAGCGCA GCCAGAACAA GGACCGCAGG AAGCTGGGCA GCCGGAATCG GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107562.1
CDS	153..2009
Translation	MELLAAAFSAACAVDHDSSTSESDARDSAAGHLPGSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	NM_001114090.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114090.2

ORF Insert Sequence:

ATGGAGCTGC TGGCCGCTGC CTTCAGCGCC GCCTGCGCCG TGGACCACGA CAGTTCCACC 
TCGGAAAGCG ACGCGCGCGA CTCGGCGGCG GGACACCTGC CCGGCAGTGA GTCGTCCTCC 
ACCCCGGGAA ATGGGGCCAC GCCCGAGGAG TGGCCGGCCC TGGCCGACAG CCCCACCACG 
CTCACCGAGG CCCTGCGGAT GATCCACCCC ATTCCCGCCG ACTCCTGGAG AAACCTCATT 
GAACAAATAG GGCTCCTGTA TCAGGAATAC CGAGATAAAT CGACTCTCCA AGAAATCGAA 
ACCAGGAGGC AACAGGATGC AGAAATAGAA GACAATACCA ATGGGTCCCC GGCCAGTGAG 
GACACCCCGG AGGAGGAAGA AGAAGAGGAG GAGGAGGAGG AGCCGGCCAG CCCACCAGAG 
AGGAAGACTC TGCCCCAGAT CTGCCTGCTC AGTAACCCCC ACTCAAGGTT CAACCTCTGG 
CAGGATCTTC CCGAGATCCG GAGCAGCGGG GTGCTTGAGA TCCTACAGCC CGAGGAGATT 
AAGCTGCAGG AGGCCATGTT CGAGCTGGTC ACTTCCGAGG CGTCCTACTA CAAGAGTCTG 
AACCTGCTCG TGTCCCACTT CATGGAGAAC GAGCGGATAA GGAAGATCCT GCACCCGTCC 
GAGGCGCACA TCCTCTTCTC CAACGTCCTG GACGTGCTGG CTGTCAGTGA GCGGTTCCTC 
CTGGAGCTGG AGCACCGGAT GGAGGAGAAC ATCGTCATCT CTGACGTGTG TGACATTGTG 
TACCGTTATG CGGCCGACCA CTTCTCTGTC TACATCACCT ACGTCAGCAA TCAGACCTAC 
CAGGAGCGGA CCTATAAGCA GCTGCTCCAG GAGAAGGCAG CTTTCCGGGA GCTGATCGCG 
CAGCTAGAGC TCGACCCCAA GTGCAGGGGG CTGCCCTTCT CCTCCTTCCT CATCCTGCCT 
TTCCAGAGGA TCACACGCCT CAAGCTGTTG GTCCAGAACA TCCTGAAGAG GGTAGAAGAG 
AGGTCTGAGC GGGAGTGCAC TGCTTTGGAT GCTCACAAGG AGCTGGAAAT GGTGGTGAAG 
GCATGCAACG AGGGCGTCAG GAAAATGAGC CGCACGGAAC AGATGATCAG CATTCAGAAG 
AAGATGGAGT TCAAGATCAA GTCGGTGCCC ATCATCTCCC ACTCCCGCTG GCTGCTGAAG 
CAGGGTGAGC TGCAGCAGAT GTCAGGCCCC AAGACCTCCC GGACCCTGAG GACCAAGAAG 
CTCTTCCACG AAATTTACCT CTTCCTGTTC AACGACCTGC TGGTGATCTG CCGGCAGATT 
CCAGGAGACA AGTACCAGGT ATTTGACTCA GCTCCGCGGG GACTGCTGCG TGTGGAGGAG 
CTGGAGGACC AGGGCCAGAC GCTGGCCAAC GTGTTCATCC TGCGGCTGCT GGAGAACGCA 
GATGACCGGG AGGCCACCTA CATGCTAAAG GCGTCCTCTC AGAGTGAGAT GAAGCGTTGG 
ATGACCTCAC TGGCCCCCAA CAGGAGGACC AAGTTTGTTT CGTTCACATC CCGGCTGCTG 
GACTGCCCCC AGGTCCAGTG CGTGCACCCA TACGTGGCTC AGCAGCCAGA CGAGCTGACG 
CTGGAGCTCG CCGACATCCT CAACATCCTG GACAAGACTG ACGACGGGTG GATCTTTGGC 
GAGCGTCTGC ACGACCAGGA GAGAGGCTGG TTCCCCAGCT CCATGACTGA GGAGATCTTG 
AATCCCAAGA TCCGGTCCCA GAACCTCAAG GAATGTTTCC GTGTCCACAA GATGGATGAC 
CCTCAGCGCA GCCAGAACAA GGACCGCAGG AAGCTGGGCA GCCGGAATCG GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGEF ( NM_001114090.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107562.1 Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2, mRNA.

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CloneID	OHu16462
Clone ID Related Accession (Same CDS sequence)	NM_001114090.2 , NM_001114090.1
Accession Version	NM_001114090.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	ephexin-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC073962.1, BX431262.1, BC031573.1 and AC016692.9. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297390.1, SRR1660805.111997.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGC TGGCCGCTGC CTTCAGCGCC GCCTGCGCCG TGGACCACGA CAGTTCCACC 
TCGGAAAGCG ACGCGCGCGA CTCGGCGGCG GGACACCTGC CCGGCAGTGA GTCGTCCTCC 
ACCCCGGGAA ATGGGGCCAC GCCCGAGGAG TGGCCGGCCC TGGCCGACAG CCCCACCACG 
CTCACCGAGG CCCTGCGGAT GATCCACCCC ATTCCCGCCG ACTCCTGGAG AAACCTCATT 
GAACAAATAG GGCTCCTGTA TCAGGAATAC CGAGATAAAT CGACTCTCCA AGAAATCGAA 
ACCAGGAGGC AACAGGATGC AGAAATAGAA GACAATACCA ATGGGTCCCC GGCCAGTGAG 
GACACCCCGG AGGAGGAAGA AGAAGAGGAG GAGGAGGAGG AGCCGGCCAG CCCACCAGAG 
AGGAAGACTC TGCCCCAGAT CTGCCTGCTC AGTAACCCCC ACTCAAGGTT CAACCTCTGG 
CAGGATCTTC CCGAGATCCG GAGCAGCGGG GTGCTTGAGA TCCTACAGCC CGAGGAGATT 
AAGCTGCAGG AGGCCATGTT CGAGCTGGTC ACTTCCGAGG CGTCCTACTA CAAGAGTCTG 
AACCTGCTCG TGTCCCACTT CATGGAGAAC GAGCGGATAA GGAAGATCCT GCACCCGTCC 
GAGGCGCACA TCCTCTTCTC CAACGTCCTG GACGTGCTGG CTGTCAGTGA GCGGTTCCTC 
CTGGAGCTGG AGCACCGGAT GGAGGAGAAC ATCGTCATCT CTGACGTGTG TGACATTGTG 
TACCGTTATG CGGCCGACCA CTTCTCTGTC TACATCACCT ACGTCAGCAA TCAGACCTAC 
CAGGAGCGGA CCTATAAGCA GCTGCTCCAG GAGAAGGCAG CTTTCCGGGA GCTGATCGCG 
CAGCTAGAGC TCGACCCCAA GTGCAGGGGG CTGCCCTTCT CCTCCTTCCT CATCCTGCCT 
TTCCAGAGGA TCACACGCCT CAAGCTGTTG GTCCAGAACA TCCTGAAGAG GGTAGAAGAG 
AGGTCTGAGC GGGAGTGCAC TGCTTTGGAT GCTCACAAGG AGCTGGAAAT GGTGGTGAAG 
GCATGCAACG AGGGCGTCAG GAAAATGAGC CGCACGGAAC AGATGATCAG CATTCAGAAG 
AAGATGGAGT TCAAGATCAA GTCGGTGCCC ATCATCTCCC ACTCCCGCTG GCTGCTGAAG 
CAGGGTGAGC TGCAGCAGAT GTCAGGCCCC AAGACCTCCC GGACCCTGAG GACCAAGAAG 
CTCTTCCACG AAATTTACCT CTTCCTGTTC AACGACCTGC TGGTGATCTG CCGGCAGATT 
CCAGGAGACA AGTACCAGGT ATTTGACTCA GCTCCGCGGG GACTGCTGCG TGTGGAGGAG 
CTGGAGGACC AGGGCCAGAC GCTGGCCAAC GTGTTCATCC TGCGGCTGCT GGAGAACGCA 
GATGACCGGG AGGCCACCTA CATGCTAAAG GCGTCCTCTC AGAGTGAGAT GAAGCGTTGG 
ATGACCTCAC TGGCCCCCAA CAGGAGGACC AAGTTTGTTT CGTTCACATC CCGGCTGCTG 
GACTGCCCCC AGGTCCAGTG CGTGCACCCA TACGTGGCTC AGCAGCCAGA CGAGCTGACG 
CTGGAGCTCG CCGACATCCT CAACATCCTG GACAAGACTG ACGACGGGTG GATCTTTGGC 
GAGCGTCTGC ACGACCAGGA GAGAGGCTGG TTCCCCAGCT CCATGACTGA GGAGATCTTG 
AATCCCAAGA TCCGGTCCCA GAACCTCAAG GAATGTTTCC GTGTCCACAA GATGGATGAC 
CCTCAGCGCA GCCAGAACAA GGACCGCAGG AAGCTGGGCA GCCGGAATCG GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107562.1
CDS	153..2009
Translation	MELLAAAFSAACAVDHDSSTSESDARDSAAGHLPGSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	NM_001114090.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114090.1

ORF Insert Sequence:

ATGGAGCTGC TGGCCGCTGC CTTCAGCGCC GCCTGCGCCG TGGACCACGA CAGTTCCACC 
TCGGAAAGCG ACGCGCGCGA CTCGGCGGCG GGACACCTGC CCGGCAGTGA GTCGTCCTCC 
ACCCCGGGAA ATGGGGCCAC GCCCGAGGAG TGGCCGGCCC TGGCCGACAG CCCCACCACG 
CTCACCGAGG CCCTGCGGAT GATCCACCCC ATTCCCGCCG ACTCCTGGAG AAACCTCATT 
GAACAAATAG GGCTCCTGTA TCAGGAATAC CGAGATAAAT CGACTCTCCA AGAAATCGAA 
ACCAGGAGGC AACAGGATGC AGAAATAGAA GACAATACCA ATGGGTCCCC GGCCAGTGAG 
GACACCCCGG AGGAGGAAGA AGAAGAGGAG GAGGAGGAGG AGCCGGCCAG CCCACCAGAG 
AGGAAGACTC TGCCCCAGAT CTGCCTGCTC AGTAACCCCC ACTCAAGGTT CAACCTCTGG 
CAGGATCTTC CCGAGATCCG GAGCAGCGGG GTGCTTGAGA TCCTACAGCC CGAGGAGATT 
AAGCTGCAGG AGGCCATGTT CGAGCTGGTC ACTTCCGAGG CGTCCTACTA CAAGAGTCTG 
AACCTGCTCG TGTCCCACTT CATGGAGAAC GAGCGGATAA GGAAGATCCT GCACCCGTCC 
GAGGCGCACA TCCTCTTCTC CAACGTCCTG GACGTGCTGG CTGTCAGTGA GCGGTTCCTC 
CTGGAGCTGG AGCACCGGAT GGAGGAGAAC ATCGTCATCT CTGACGTGTG TGACATTGTG 
TACCGTTATG CGGCCGACCA CTTCTCTGTC TACATCACCT ACGTCAGCAA TCAGACCTAC 
CAGGAGCGGA CCTATAAGCA GCTGCTCCAG GAGAAGGCAG CTTTCCGGGA GCTGATCGCG 
CAGCTAGAGC TCGACCCCAA GTGCAGGGGG CTGCCCTTCT CCTCCTTCCT CATCCTGCCT 
TTCCAGAGGA TCACACGCCT CAAGCTGTTG GTCCAGAACA TCCTGAAGAG GGTAGAAGAG 
AGGTCTGAGC GGGAGTGCAC TGCTTTGGAT GCTCACAAGG AGCTGGAAAT GGTGGTGAAG 
GCATGCAACG AGGGCGTCAG GAAAATGAGC CGCACGGAAC AGATGATCAG CATTCAGAAG 
AAGATGGAGT TCAAGATCAA GTCGGTGCCC ATCATCTCCC ACTCCCGCTG GCTGCTGAAG 
CAGGGTGAGC TGCAGCAGAT GTCAGGCCCC AAGACCTCCC GGACCCTGAG GACCAAGAAG 
CTCTTCCACG AAATTTACCT CTTCCTGTTC AACGACCTGC TGGTGATCTG CCGGCAGATT 
CCAGGAGACA AGTACCAGGT ATTTGACTCA GCTCCGCGGG GACTGCTGCG TGTGGAGGAG 
CTGGAGGACC AGGGCCAGAC GCTGGCCAAC GTGTTCATCC TGCGGCTGCT GGAGAACGCA 
GATGACCGGG AGGCCACCTA CATGCTAAAG GCGTCCTCTC AGAGTGAGAT GAAGCGTTGG 
ATGACCTCAC TGGCCCCCAA CAGGAGGACC AAGTTTGTTT CGTTCACATC CCGGCTGCTG 
GACTGCCCCC AGGTCCAGTG CGTGCACCCA TACGTGGCTC AGCAGCCAGA CGAGCTGACG 
CTGGAGCTCG CCGACATCCT CAACATCCTG GACAAGACTG ACGACGGGTG GATCTTTGGC 
GAGCGTCTGC ACGACCAGGA GAGAGGCTGG TTCCCCAGCT CCATGACTGA GGAGATCTTG 
AATCCCAAGA TCCGGTCCCA GAACCTCAAG GAATGTTTCC GTGTCCACAA GATGGATGAC 
CCTCAGCGCA GCCAGAACAA GGACCGCAGG AAGCTGGGCA GCCGGAATCG GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGEF ( XM_011510924.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509226.1 Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X2, mRNA.

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CloneID	OHu66488
Clone ID Related Accession (Same CDS sequence)	XM_011510924.1 , XM_011510925.1
Accession Version	XM_011510924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ephexin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGAGC TGGTCACTTC CGAGGCGTCC TACTACAAGA GTCTGAACCT GCTCGTGTCC 
CACTTCATGG AGAACGAGCG GATAAGGAAG ATCCTGCACC CGTCCGAGGC GCACATCCTC 
TTCTCCAACG TCCTGGACGT GCTGGCTGTC AGTGAGCGGT TCCTCCTGGA GCTGGAGCAC 
CGGATGGAGG AGAACATCGT CATCTCTGAC GTGTGTGACA TTGTGTACCG TTATGCGGCC 
GACCACTTCT CTGTCTACAT CACCTACGTC AGCAATCAGA CCTACCAGGA GCGGACCTAT 
AAGCAGCTGC TCCAGGAGAA GGCAGCTTTC CGGGAGCTGA TCGCGCAGCT AGAGCTCGAC 
CCCAAGTGCA GGGGGCTGCC CTTCTCCTCC TTCCTCATCC TGCCTTTCCA GAGGATCACA 
CGCCTCAAGC TGTTGGTCCA GAACATCCTG AAGAGGGTAG AAGAGAGGTC TGAGCGGGAG 
TGCACTGCTT TGGATGCTCA CAAGGAGCTG GAAATGGTGG TGAAGGCATG CAACGAGGGC 
GTCAGGAAAA TGAGCCGCAC GGAACAGATG ATCAGCATTC AGAAGAAGAT GGAGTTCAAG 
ATCAAGTCGG TGCCCATCAT CTCCCACTCC CGCTGGCTGC TGAAGCAGGG TGAGCTGCAG 
CAGATGTCAG GCCCCAAGAC CTCCCGGACC CTGAGGACCA AGAAGCTCTT CCACGAAATT 
TACCTCTTCC TGTTCAACGA CCTGCTGGTG ATCTGCCGGC AGATTCCAGG AGACAAGTAC 
CAGGTATTTG ACTCAGCTCC GCGGGGACTG CTGCGTGTGG AGGAGCTGGA GGACCAGGGC 
CAGACGCTGG CCAACGTGTT CATCCTGCGG CTGCTGGAGA ACGCAGATGA CCGGGAGGCC 
ACCTACATGC TAAAGGCGTC CTCTCAGAGT GAGATGAAGC GTTGGATGAC CTCACTGGCC 
CCCAACAGGA GGACCAAGTT TGTTTCGTTC ACATCCCGGC TGCTGGACTG CCCCCAGGTC 
CAGTGCGTGC ACCCATACGT GGCTCAGCAG CCAGACGAGC TGACGCTGGA GCTCGCCGAC 
ATCCTCAACA TCCTGGACAA GACTGACGAC GGGTGGATCT TTGGCGAGCG TCTGCACGAC 
CAGGAGAGAG GCTGGTTCCC CAGCTCCATG ACTGAGGAGA TCTTGAATCC CAAGATCCGG 
TCCCAGAACC TCAAGGAATG TTTCCGTGTC CACAAGATGG ATGACCCTCA GCGCAGCCAG 
AACAAGGACC GCAGGAAGCT GGGCAGCCGG AATCGGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509226.1
CDS	79..1380
Translation	MFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	XM_011510924.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510924.1

ORF Insert Sequence:

ATGTTCGAGC TGGTCACTTC CGAGGCGTCC TACTACAAGA GTCTGAACCT GCTCGTGTCC 
CACTTCATGG AGAACGAGCG GATAAGGAAG ATCCTGCACC CGTCCGAGGC GCACATCCTC 
TTCTCCAACG TCCTGGACGT GCTGGCTGTC AGTGAGCGGT TCCTCCTGGA GCTGGAGCAC 
CGGATGGAGG AGAACATCGT CATCTCTGAC GTGTGTGACA TTGTGTACCG TTATGCGGCC 
GACCACTTCT CTGTCTACAT CACCTACGTC AGCAATCAGA CCTACCAGGA GCGGACCTAT 
AAGCAGCTGC TCCAGGAGAA GGCAGCTTTC CGGGAGCTGA TCGCGCAGCT AGAGCTCGAC 
CCCAAGTGCA GGGGGCTGCC CTTCTCCTCC TTCCTCATCC TGCCTTTCCA GAGGATCACA 
CGCCTCAAGC TGTTGGTCCA GAACATCCTG AAGAGGGTAG AAGAGAGGTC TGAGCGGGAG 
TGCACTGCTT TGGATGCTCA CAAGGAGCTG GAAATGGTGG TGAAGGCATG CAACGAGGGC 
GTCAGGAAAA TGAGCCGCAC GGAACAGATG ATCAGCATTC AGAAGAAGAT GGAGTTCAAG 
ATCAAGTCGG TGCCCATCAT CTCCCACTCC CGCTGGCTGC TGAAGCAGGG TGAGCTGCAG 
CAGATGTCAG GCCCCAAGAC CTCCCGGACC CTGAGGACCA AGAAGCTCTT CCACGAAATT 
TACCTCTTCC TGTTCAACGA CCTGCTGGTG ATCTGCCGGC AGATTCCAGG AGACAAGTAC 
CAGGTATTTG ACTCAGCTCC GCGGGGACTG CTGCGTGTGG AGGAGCTGGA GGACCAGGGC 
CAGACGCTGG CCAACGTGTT CATCCTGCGG CTGCTGGAGA ACGCAGATGA CCGGGAGGCC 
ACCTACATGC TAAAGGCGTC CTCTCAGAGT GAGATGAAGC GTTGGATGAC CTCACTGGCC 
CCCAACAGGA GGACCAAGTT TGTTTCGTTC ACATCCCGGC TGCTGGACTG CCCCCAGGTC 
CAGTGCGTGC ACCCATACGT GGCTCAGCAG CCAGACGAGC TGACGCTGGA GCTCGCCGAC 
ATCCTCAACA TCCTGGACAA GACTGACGAC GGGTGGATCT TTGGCGAGCG TCTGCACGAC 
CAGGAGAGAG GCTGGTTCCC CAGCTCCATG ACTGAGGAGA TCTTGAATCC CAAGATCCGG 
TCCCAGAACC TCAAGGAATG TTTCCGTGTC CACAAGATGG ATGACCCTCA GCGCAGCCAG 
AACAAGGACC GCAGGAAGCT GGGCAGCCGG AATCGGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGEF ( XM_011510925.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509227.1 Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X3, mRNA.

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CloneID	OHu66488
Clone ID Related Accession (Same CDS sequence)	XM_011510924.1 , XM_011510925.1
Accession Version	XM_011510925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ephexin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGAGC TGGTCACTTC CGAGGCGTCC TACTACAAGA GTCTGAACCT GCTCGTGTCC 
CACTTCATGG AGAACGAGCG GATAAGGAAG ATCCTGCACC CGTCCGAGGC GCACATCCTC 
TTCTCCAACG TCCTGGACGT GCTGGCTGTC AGTGAGCGGT TCCTCCTGGA GCTGGAGCAC 
CGGATGGAGG AGAACATCGT CATCTCTGAC GTGTGTGACA TTGTGTACCG TTATGCGGCC 
GACCACTTCT CTGTCTACAT CACCTACGTC AGCAATCAGA CCTACCAGGA GCGGACCTAT 
AAGCAGCTGC TCCAGGAGAA GGCAGCTTTC CGGGAGCTGA TCGCGCAGCT AGAGCTCGAC 
CCCAAGTGCA GGGGGCTGCC CTTCTCCTCC TTCCTCATCC TGCCTTTCCA GAGGATCACA 
CGCCTCAAGC TGTTGGTCCA GAACATCCTG AAGAGGGTAG AAGAGAGGTC TGAGCGGGAG 
TGCACTGCTT TGGATGCTCA CAAGGAGCTG GAAATGGTGG TGAAGGCATG CAACGAGGGC 
GTCAGGAAAA TGAGCCGCAC GGAACAGATG ATCAGCATTC AGAAGAAGAT GGAGTTCAAG 
ATCAAGTCGG TGCCCATCAT CTCCCACTCC CGCTGGCTGC TGAAGCAGGG TGAGCTGCAG 
CAGATGTCAG GCCCCAAGAC CTCCCGGACC CTGAGGACCA AGAAGCTCTT CCACGAAATT 
TACCTCTTCC TGTTCAACGA CCTGCTGGTG ATCTGCCGGC AGATTCCAGG AGACAAGTAC 
CAGGTATTTG ACTCAGCTCC GCGGGGACTG CTGCGTGTGG AGGAGCTGGA GGACCAGGGC 
CAGACGCTGG CCAACGTGTT CATCCTGCGG CTGCTGGAGA ACGCAGATGA CCGGGAGGCC 
ACCTACATGC TAAAGGCGTC CTCTCAGAGT GAGATGAAGC GTTGGATGAC CTCACTGGCC 
CCCAACAGGA GGACCAAGTT TGTTTCGTTC ACATCCCGGC TGCTGGACTG CCCCCAGGTC 
CAGTGCGTGC ACCCATACGT GGCTCAGCAG CCAGACGAGC TGACGCTGGA GCTCGCCGAC 
ATCCTCAACA TCCTGGACAA GACTGACGAC GGGTGGATCT TTGGCGAGCG TCTGCACGAC 
CAGGAGAGAG GCTGGTTCCC CAGCTCCATG ACTGAGGAGA TCTTGAATCC CAAGATCCGG 
TCCCAGAACC TCAAGGAATG TTTCCGTGTC CACAAGATGG ATGACCCTCA GCGCAGCCAG 
AACAAGGACC GCAGGAAGCT GGGCAGCCGG AATCGGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509227.1
CDS	83..1384
Translation	MFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	XM_011510925.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510925.1

ORF Insert Sequence:

ATGTTCGAGC TGGTCACTTC CGAGGCGTCC TACTACAAGA GTCTGAACCT GCTCGTGTCC 
CACTTCATGG AGAACGAGCG GATAAGGAAG ATCCTGCACC CGTCCGAGGC GCACATCCTC 
TTCTCCAACG TCCTGGACGT GCTGGCTGTC AGTGAGCGGT TCCTCCTGGA GCTGGAGCAC 
CGGATGGAGG AGAACATCGT CATCTCTGAC GTGTGTGACA TTGTGTACCG TTATGCGGCC 
GACCACTTCT CTGTCTACAT CACCTACGTC AGCAATCAGA CCTACCAGGA GCGGACCTAT 
AAGCAGCTGC TCCAGGAGAA GGCAGCTTTC CGGGAGCTGA TCGCGCAGCT AGAGCTCGAC 
CCCAAGTGCA GGGGGCTGCC CTTCTCCTCC TTCCTCATCC TGCCTTTCCA GAGGATCACA 
CGCCTCAAGC TGTTGGTCCA GAACATCCTG AAGAGGGTAG AAGAGAGGTC TGAGCGGGAG 
TGCACTGCTT TGGATGCTCA CAAGGAGCTG GAAATGGTGG TGAAGGCATG CAACGAGGGC 
GTCAGGAAAA TGAGCCGCAC GGAACAGATG ATCAGCATTC AGAAGAAGAT GGAGTTCAAG 
ATCAAGTCGG TGCCCATCAT CTCCCACTCC CGCTGGCTGC TGAAGCAGGG TGAGCTGCAG 
CAGATGTCAG GCCCCAAGAC CTCCCGGACC CTGAGGACCA AGAAGCTCTT CCACGAAATT 
TACCTCTTCC TGTTCAACGA CCTGCTGGTG ATCTGCCGGC AGATTCCAGG AGACAAGTAC 
CAGGTATTTG ACTCAGCTCC GCGGGGACTG CTGCGTGTGG AGGAGCTGGA GGACCAGGGC 
CAGACGCTGG CCAACGTGTT CATCCTGCGG CTGCTGGAGA ACGCAGATGA CCGGGAGGCC 
ACCTACATGC TAAAGGCGTC CTCTCAGAGT GAGATGAAGC GTTGGATGAC CTCACTGGCC 
CCCAACAGGA GGACCAAGTT TGTTTCGTTC ACATCCCGGC TGCTGGACTG CCCCCAGGTC 
CAGTGCGTGC ACCCATACGT GGCTCAGCAG CCAGACGAGC TGACGCTGGA GCTCGCCGAC 
ATCCTCAACA TCCTGGACAA GACTGACGAC GGGTGGATCT TTGGCGAGCG TCTGCACGAC 
CAGGAGAGAG GCTGGTTCCC CAGCTCCATG ACTGAGGAGA TCTTGAATCC CAAGATCCGG 
TCCCAGAACC TCAAGGAATG TTTCCGTGTC CACAAGATGG ATGACCCTCA GCGCAGCCAG 
AACAAGGACC GCAGGAAGCT GGGCAGCCGG AATCGGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGEF ( NM_019850.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_062824.2 Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1, mRNA.

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CloneID	OHu16623
Clone ID Related Accession (Same CDS sequence)	XM_011510923.2 , NM_019850.2 , NM_019850.3 , XM_011510923.3
Accession Version	NM_019850.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	ephexin-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB242442.1, DC388448.1, BC073962.1, BX431262.1, BC031573.1 and AC016692.9. On Jan 29, 2008 this sequence version replaced NM_019850.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031573.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACCA GGGAATCTGA AGATTTGGAA AAGACCCGGA GGAAATCAGC AAGTGATCAA 
TGGAACACTG ATAATGAACC AGCCAAGGTG AAACCTGAGT TACTCCCAGA AAAAGAGGAG 
ACTTCTCAAG CTGACCAGGA TATCCAAGAC AAAGAGCCTC ATTGCCACAT CCCAATTAAG 
AGAAATTCCA TCTTCAATCG CTCCATAAGA CGCAAAAGCA AAGCCAAGGC CAGAGACAAC 
CCCGAACGGA ACGCCAGCTG CCTGGCAGAT TCACAGGACA ATGGAAAATC TGTAAATGAG 
CCCCTGACCT TGAATATCCC CTGGAGCAGA ATGCCTCCTT GCAGAACAGC AATGCAGACA 
GACCCAGGAG CCCAGGAAAT GAGTGAGTCG TCCTCCACCC CGGGAAATGG GGCCACGCCC 
GAGGAGTGGC CGGCCCTGGC CGACAGCCCC ACCACGCTCA CCGAGGCCCT GCGGATGATC 
CACCCCATTC CCGCCGACTC CTGGAGAAAC CTCATTGAAC AAATAGGGCT CCTGTATCAG 
GAATACCGAG ATAAATCGAC TCTCCAAGAA ATCGAAACCA GGAGGCAACA GGATGCAGAA 
ATAGAAGACA ATACCAATGG GTCCCCGGCC AGTGAGGACA CCCCGGAGGA GGAAGAAGAA 
GAGGAGGAGG AGGAGGAGCC GGCCAGCCCA CCAGAGAGGA AGACTCTGCC CCAGATCTGC 
CTGCTCAGTA ACCCCCACTC AAGGTTCAAC CTCTGGCAGG ATCTTCCCGA GATCCGGAGC 
AGCGGGGTGC TTGAGATCCT ACAGCCCGAG GAGATTAAGC TGCAGGAGGC CATGTTCGAG 
CTGGTCACTT CCGAGGCGTC CTACTACAAG AGTCTGAACC TGCTCGTGTC CCACTTCATG 
GAGAACGAGC GGATAAGGAA GATCCTGCAC CCGTCCGAGG CGCACATCCT CTTCTCCAAC 
GTCCTGGACG TGCTGGCTGT CAGTGAGCGG TTCCTCCTGG AGCTGGAGCA CCGGATGGAG 
GAGAACATCG TCATCTCTGA CGTGTGTGAC ATTGTGTACC GTTATGCGGC CGACCACTTC 
TCTGTCTACA TCACCTACGT CAGCAATCAG ACCTACCAGG AGCGGACCTA TAAGCAGCTG 
CTCCAGGAGA AGGCAGCTTT CCGGGAGCTG ATCGCGCAGC TAGAGCTCGA CCCCAAGTGC 
AGGGGGCTGC CCTTCTCCTC CTTCCTCATC CTGCCTTTCC AGAGGATCAC ACGCCTCAAG 
CTGTTGGTCC AGAACATCCT GAAGAGGGTA GAAGAGAGGT CTGAGCGGGA GTGCACTGCT 
TTGGATGCTC ACAAGGAGCT GGAAATGGTG GTGAAGGCAT GCAACGAGGG CGTCAGGAAA 
ATGAGCCGCA CGGAACAGAT GATCAGCATT CAGAAGAAGA TGGAGTTCAA GATCAAGTCG 
GTGCCCATCA TCTCCCACTC CCGCTGGCTG CTGAAGCAGG GTGAGCTGCA GCAGATGTCA 
GGCCCCAAGA CCTCCCGGAC CCTGAGGACC AAGAAGCTCT TCCACGAAAT TTACCTCTTC 
CTGTTCAACG ACCTGCTGGT GATCTGCCGG CAGATTCCAG GAGACAAGTA CCAGGTATTT 
GACTCAGCTC CGCGGGGACT GCTGCGTGTG GAGGAGCTGG AGGACCAGGG CCAGACGCTG 
GCCAACGTGT TCATCCTGCG GCTGCTGGAG AACGCAGATG ACCGGGAGGC CACCTACATG 
CTAAAGGCGT CCTCTCAGAG TGAGATGAAG CGTTGGATGA CCTCACTGGC CCCCAACAGG 
AGGACCAAGT TTGTTTCGTT CACATCCCGG CTGCTGGACT GCCCCCAGGT CCAGTGCGTG 
CACCCATACG TGGCTCAGCA GCCAGACGAG CTGACGCTGG AGCTCGCCGA CATCCTCAAC 
ATCCTGGACA AGACTGACGA CGGGTGGATC TTTGGCGAGC GTCTGCACGA CCAGGAGAGA 
GGCTGGTTCC CCAGCTCCAT GACTGAGGAG ATCTTGAATC CCAAGATCCG GTCCCAGAAC 
CTCAAGGAAT GTTTCCGTGT CCACAAGATG GATGACCCTC AGCGCAGCCA GAACAAGGAC 
CGCAGGAAGC TGGGCAGCCG GAATCGGCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062824.2
CDS	249..2381
Translation	METRESEDLEKTRRKSASDQWNTDNEPAKVKPELLPEKEETSQADQDIQDKEPHCHIPIKRNSIFNRSIRRKSKAKARDNPERNASCLADSQDNGKSVNEPLTLNIPWSRMPPCRTAMQTDPGAQEMSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	NM_019850.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019850.2

ORF Insert Sequence:

ATGGAGACCA GGGAATCTGA AGATTTGGAA AAGACCCGGA GGAAATCAGC AAGTGATCAA 
TGGAACACTG ATAATGAACC AGCCAAGGTG AAACCTGAGT TACTCCCAGA AAAAGAGGAG 
ACTTCTCAAG CTGACCAGGA TATCCAAGAC AAAGAGCCTC ATTGCCACAT CCCAATTAAG 
AGAAATTCCA TCTTCAATCG CTCCATAAGA CGCAAAAGCA AAGCCAAGGC CAGAGACAAC 
CCCGAACGGA ACGCCAGCTG CCTGGCAGAT TCACAGGACA ATGGAAAATC TGTAAATGAG 
CCCCTGACCT TGAATATCCC CTGGAGCAGA ATGCCTCCTT GCAGAACAGC AATGCAGACA 
GACCCAGGAG CCCAGGAAAT GAGTGAGTCG TCCTCCACCC CGGGAAATGG GGCCACGCCC 
GAGGAGTGGC CGGCCCTGGC CGACAGCCCC ACCACGCTCA CCGAGGCCCT GCGGATGATC 
CACCCCATTC CCGCCGACTC CTGGAGAAAC CTCATTGAAC AAATAGGGCT CCTGTATCAG 
GAATACCGAG ATAAATCGAC TCTCCAAGAA ATCGAAACCA GGAGGCAACA GGATGCAGAA 
ATAGAAGACA ATACCAATGG GTCCCCGGCC AGTGAGGACA CCCCGGAGGA GGAAGAAGAA 
GAGGAGGAGG AGGAGGAGCC GGCCAGCCCA CCAGAGAGGA AGACTCTGCC CCAGATCTGC 
CTGCTCAGTA ACCCCCACTC AAGGTTCAAC CTCTGGCAGG ATCTTCCCGA GATCCGGAGC 
AGCGGGGTGC TTGAGATCCT ACAGCCCGAG GAGATTAAGC TGCAGGAGGC CATGTTCGAG 
CTGGTCACTT CCGAGGCGTC CTACTACAAG AGTCTGAACC TGCTCGTGTC CCACTTCATG 
GAGAACGAGC GGATAAGGAA GATCCTGCAC CCGTCCGAGG CGCACATCCT CTTCTCCAAC 
GTCCTGGACG TGCTGGCTGT CAGTGAGCGG TTCCTCCTGG AGCTGGAGCA CCGGATGGAG 
GAGAACATCG TCATCTCTGA CGTGTGTGAC ATTGTGTACC GTTATGCGGC CGACCACTTC 
TCTGTCTACA TCACCTACGT CAGCAATCAG ACCTACCAGG AGCGGACCTA TAAGCAGCTG 
CTCCAGGAGA AGGCAGCTTT CCGGGAGCTG ATCGCGCAGC TAGAGCTCGA CCCCAAGTGC 
AGGGGGCTGC CCTTCTCCTC CTTCCTCATC CTGCCTTTCC AGAGGATCAC ACGCCTCAAG 
CTGTTGGTCC AGAACATCCT GAAGAGGGTA GAAGAGAGGT CTGAGCGGGA GTGCACTGCT 
TTGGATGCTC ACAAGGAGCT GGAAATGGTG GTGAAGGCAT GCAACGAGGG CGTCAGGAAA 
ATGAGCCGCA CGGAACAGAT GATCAGCATT CAGAAGAAGA TGGAGTTCAA GATCAAGTCG 
GTGCCCATCA TCTCCCACTC CCGCTGGCTG CTGAAGCAGG GTGAGCTGCA GCAGATGTCA 
GGCCCCAAGA CCTCCCGGAC CCTGAGGACC AAGAAGCTCT TCCACGAAAT TTACCTCTTC 
CTGTTCAACG ACCTGCTGGT GATCTGCCGG CAGATTCCAG GAGACAAGTA CCAGGTATTT 
GACTCAGCTC CGCGGGGACT GCTGCGTGTG GAGGAGCTGG AGGACCAGGG CCAGACGCTG 
GCCAACGTGT TCATCCTGCG GCTGCTGGAG AACGCAGATG ACCGGGAGGC CACCTACATG 
CTAAAGGCGT CCTCTCAGAG TGAGATGAAG CGTTGGATGA CCTCACTGGC CCCCAACAGG 
AGGACCAAGT TTGTTTCGTT CACATCCCGG CTGCTGGACT GCCCCCAGGT CCAGTGCGTG 
CACCCATACG TGGCTCAGCA GCCAGACGAG CTGACGCTGG AGCTCGCCGA CATCCTCAAC 
ATCCTGGACA AGACTGACGA CGGGTGGATC TTTGGCGAGC GTCTGCACGA CCAGGAGAGA 
GGCTGGTTCC CCAGCTCCAT GACTGAGGAG ATCTTGAATC CCAAGATCCG GTCCCAGAAC 
CTCAAGGAAT GTTTCCGTGT CCACAAGATG GATGACCCTC AGCGCAGCCA GAACAAGGAC 
CGCAGGAAGC TGGGCAGCCG GAATCGGCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGEF ( XM_011510923.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509225.1 Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X1, mRNA.

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CloneID	OHu16623
Clone ID Related Accession (Same CDS sequence)	XM_011510923.2 , NM_019850.2 , NM_019850.3 , XM_011510923.3
Accession Version	XM_011510923.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ephexin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCA GGGAATCTGA AGATTTGGAA AAGACCCGGA GGAAATCAGC AAGTGATCAA 
TGGAACACTG ATAATGAACC AGCCAAGGTG AAACCTGAGT TACTCCCAGA AAAAGAGGAG 
ACTTCTCAAG CTGACCAGGA TATCCAAGAC AAAGAGCCTC ATTGCCACAT CCCAATTAAG 
AGAAATTCCA TCTTCAATCG CTCCATAAGA CGCAAAAGCA AAGCCAAGGC CAGAGACAAC 
CCCGAACGGA ACGCCAGCTG CCTGGCAGAT TCACAGGACA ATGGAAAATC TGTAAATGAG 
CCCCTGACCT TGAATATCCC CTGGAGCAGA ATGCCTCCTT GCAGAACAGC AATGCAGACA 
GACCCAGGAG CCCAGGAAAT GAGTGAGTCG TCCTCCACCC CGGGAAATGG GGCCACGCCC 
GAGGAGTGGC CGGCCCTGGC CGACAGCCCC ACCACGCTCA CCGAGGCCCT GCGGATGATC 
CACCCCATTC CCGCCGACTC CTGGAGAAAC CTCATTGAAC AAATAGGGCT CCTGTATCAG 
GAATACCGAG ATAAATCGAC TCTCCAAGAA ATCGAAACCA GGAGGCAACA GGATGCAGAA 
ATAGAAGACA ATACCAATGG GTCCCCGGCC AGTGAGGACA CCCCGGAGGA GGAAGAAGAA 
GAGGAGGAGG AGGAGGAGCC GGCCAGCCCA CCAGAGAGGA AGACTCTGCC CCAGATCTGC 
CTGCTCAGTA ACCCCCACTC AAGGTTCAAC CTCTGGCAGG ATCTTCCCGA GATCCGGAGC 
AGCGGGGTGC TTGAGATCCT ACAGCCCGAG GAGATTAAGC TGCAGGAGGC CATGTTCGAG 
CTGGTCACTT CCGAGGCGTC CTACTACAAG AGTCTGAACC TGCTCGTGTC CCACTTCATG 
GAGAACGAGC GGATAAGGAA GATCCTGCAC CCGTCCGAGG CGCACATCCT CTTCTCCAAC 
GTCCTGGACG TGCTGGCTGT CAGTGAGCGG TTCCTCCTGG AGCTGGAGCA CCGGATGGAG 
GAGAACATCG TCATCTCTGA CGTGTGTGAC ATTGTGTACC GTTATGCGGC CGACCACTTC 
TCTGTCTACA TCACCTACGT CAGCAATCAG ACCTACCAGG AGCGGACCTA TAAGCAGCTG 
CTCCAGGAGA AGGCAGCTTT CCGGGAGCTG ATCGCGCAGC TAGAGCTCGA CCCCAAGTGC 
AGGGGGCTGC CCTTCTCCTC CTTCCTCATC CTGCCTTTCC AGAGGATCAC ACGCCTCAAG 
CTGTTGGTCC AGAACATCCT GAAGAGGGTA GAAGAGAGGT CTGAGCGGGA GTGCACTGCT 
TTGGATGCTC ACAAGGAGCT GGAAATGGTG GTGAAGGCAT GCAACGAGGG CGTCAGGAAA 
ATGAGCCGCA CGGAACAGAT GATCAGCATT CAGAAGAAGA TGGAGTTCAA GATCAAGTCG 
GTGCCCATCA TCTCCCACTC CCGCTGGCTG CTGAAGCAGG GTGAGCTGCA GCAGATGTCA 
GGCCCCAAGA CCTCCCGGAC CCTGAGGACC AAGAAGCTCT TCCACGAAAT TTACCTCTTC 
CTGTTCAACG ACCTGCTGGT GATCTGCCGG CAGATTCCAG GAGACAAGTA CCAGGTATTT 
GACTCAGCTC CGCGGGGACT GCTGCGTGTG GAGGAGCTGG AGGACCAGGG CCAGACGCTG 
GCCAACGTGT TCATCCTGCG GCTGCTGGAG AACGCAGATG ACCGGGAGGC CACCTACATG 
CTAAAGGCGT CCTCTCAGAG TGAGATGAAG CGTTGGATGA CCTCACTGGC CCCCAACAGG 
AGGACCAAGT TTGTTTCGTT CACATCCCGG CTGCTGGACT GCCCCCAGGT CCAGTGCGTG 
CACCCATACG TGGCTCAGCA GCCAGACGAG CTGACGCTGG AGCTCGCCGA CATCCTCAAC 
ATCCTGGACA AGACTGACGA CGGGTGGATC TTTGGCGAGC GTCTGCACGA CCAGGAGAGA 
GGCTGGTTCC CCAGCTCCAT GACTGAGGAG ATCTTGAATC CCAAGATCCG GTCCCAGAAC 
CTCAAGGAAT GTTTCCGTGT CCACAAGATG GATGACCCTC AGCGCAGCCA GAACAAGGAC 
CGCAGGAAGC TGGGCAGCCG GAATCGGCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509225.1
CDS	215..2347
Translation	METRESEDLEKTRRKSASDQWNTDNEPAKVKPELLPEKEETSQADQDIQDKEPHCHIPIKRNSIFNRSIRRKSKAKARDNPERNASCLADSQDNGKSVNEPLTLNIPWSRMPPCRTAMQTDPGAQEMSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	XM_011510923.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510923.2

ORF Insert Sequence:

ATGGAGACCA GGGAATCTGA AGATTTGGAA AAGACCCGGA GGAAATCAGC AAGTGATCAA 
TGGAACACTG ATAATGAACC AGCCAAGGTG AAACCTGAGT TACTCCCAGA AAAAGAGGAG 
ACTTCTCAAG CTGACCAGGA TATCCAAGAC AAAGAGCCTC ATTGCCACAT CCCAATTAAG 
AGAAATTCCA TCTTCAATCG CTCCATAAGA CGCAAAAGCA AAGCCAAGGC CAGAGACAAC 
CCCGAACGGA ACGCCAGCTG CCTGGCAGAT TCACAGGACA ATGGAAAATC TGTAAATGAG 
CCCCTGACCT TGAATATCCC CTGGAGCAGA ATGCCTCCTT GCAGAACAGC AATGCAGACA 
GACCCAGGAG CCCAGGAAAT GAGTGAGTCG TCCTCCACCC CGGGAAATGG GGCCACGCCC 
GAGGAGTGGC CGGCCCTGGC CGACAGCCCC ACCACGCTCA CCGAGGCCCT GCGGATGATC 
CACCCCATTC CCGCCGACTC CTGGAGAAAC CTCATTGAAC AAATAGGGCT CCTGTATCAG 
GAATACCGAG ATAAATCGAC TCTCCAAGAA ATCGAAACCA GGAGGCAACA GGATGCAGAA 
ATAGAAGACA ATACCAATGG GTCCCCGGCC AGTGAGGACA CCCCGGAGGA GGAAGAAGAA 
GAGGAGGAGG AGGAGGAGCC GGCCAGCCCA CCAGAGAGGA AGACTCTGCC CCAGATCTGC 
CTGCTCAGTA ACCCCCACTC AAGGTTCAAC CTCTGGCAGG ATCTTCCCGA GATCCGGAGC 
AGCGGGGTGC TTGAGATCCT ACAGCCCGAG GAGATTAAGC TGCAGGAGGC CATGTTCGAG 
CTGGTCACTT CCGAGGCGTC CTACTACAAG AGTCTGAACC TGCTCGTGTC CCACTTCATG 
GAGAACGAGC GGATAAGGAA GATCCTGCAC CCGTCCGAGG CGCACATCCT CTTCTCCAAC 
GTCCTGGACG TGCTGGCTGT CAGTGAGCGG TTCCTCCTGG AGCTGGAGCA CCGGATGGAG 
GAGAACATCG TCATCTCTGA CGTGTGTGAC ATTGTGTACC GTTATGCGGC CGACCACTTC 
TCTGTCTACA TCACCTACGT CAGCAATCAG ACCTACCAGG AGCGGACCTA TAAGCAGCTG 
CTCCAGGAGA AGGCAGCTTT CCGGGAGCTG ATCGCGCAGC TAGAGCTCGA CCCCAAGTGC 
AGGGGGCTGC CCTTCTCCTC CTTCCTCATC CTGCCTTTCC AGAGGATCAC ACGCCTCAAG 
CTGTTGGTCC AGAACATCCT GAAGAGGGTA GAAGAGAGGT CTGAGCGGGA GTGCACTGCT 
TTGGATGCTC ACAAGGAGCT GGAAATGGTG GTGAAGGCAT GCAACGAGGG CGTCAGGAAA 
ATGAGCCGCA CGGAACAGAT GATCAGCATT CAGAAGAAGA TGGAGTTCAA GATCAAGTCG 
GTGCCCATCA TCTCCCACTC CCGCTGGCTG CTGAAGCAGG GTGAGCTGCA GCAGATGTCA 
GGCCCCAAGA CCTCCCGGAC CCTGAGGACC AAGAAGCTCT TCCACGAAAT TTACCTCTTC 
CTGTTCAACG ACCTGCTGGT GATCTGCCGG CAGATTCCAG GAGACAAGTA CCAGGTATTT 
GACTCAGCTC CGCGGGGACT GCTGCGTGTG GAGGAGCTGG AGGACCAGGG CCAGACGCTG 
GCCAACGTGT TCATCCTGCG GCTGCTGGAG AACGCAGATG ACCGGGAGGC CACCTACATG 
CTAAAGGCGT CCTCTCAGAG TGAGATGAAG CGTTGGATGA CCTCACTGGC CCCCAACAGG 
AGGACCAAGT TTGTTTCGTT CACATCCCGG CTGCTGGACT GCCCCCAGGT CCAGTGCGTG 
CACCCATACG TGGCTCAGCA GCCAGACGAG CTGACGCTGG AGCTCGCCGA CATCCTCAAC 
ATCCTGGACA AGACTGACGA CGGGTGGATC TTTGGCGAGC GTCTGCACGA CCAGGAGAGA 
GGCTGGTTCC CCAGCTCCAT GACTGAGGAG ATCTTGAATC CCAAGATCCG GTCCCAGAAC 
CTCAAGGAAT GTTTCCGTGT CCACAAGATG GATGACCCTC AGCGCAGCCA GAACAAGGAC 
CGCAGGAAGC TGGGCAGCCG GAATCGGCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGEF ( XM_011510923.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509225.1 Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X1, mRNA.

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CloneID	OHu16623
Clone ID Related Accession (Same CDS sequence)	XM_011510923.2 , NM_019850.2 , NM_019850.3 , XM_011510923.3
Accession Version	XM_011510923.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ephexin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCA GGGAATCTGA AGATTTGGAA AAGACCCGGA GGAAATCAGC AAGTGATCAA 
TGGAACACTG ATAATGAACC AGCCAAGGTG AAACCTGAGT TACTCCCAGA AAAAGAGGAG 
ACTTCTCAAG CTGACCAGGA TATCCAAGAC AAAGAGCCTC ATTGCCACAT CCCAATTAAG 
AGAAATTCCA TCTTCAATCG CTCCATAAGA CGCAAAAGCA AAGCCAAGGC CAGAGACAAC 
CCCGAACGGA ACGCCAGCTG CCTGGCAGAT TCACAGGACA ATGGAAAATC TGTAAATGAG 
CCCCTGACCT TGAATATCCC CTGGAGCAGA ATGCCTCCTT GCAGAACAGC AATGCAGACA 
GACCCAGGAG CCCAGGAAAT GAGTGAGTCG TCCTCCACCC CGGGAAATGG GGCCACGCCC 
GAGGAGTGGC CGGCCCTGGC CGACAGCCCC ACCACGCTCA CCGAGGCCCT GCGGATGATC 
CACCCCATTC CCGCCGACTC CTGGAGAAAC CTCATTGAAC AAATAGGGCT CCTGTATCAG 
GAATACCGAG ATAAATCGAC TCTCCAAGAA ATCGAAACCA GGAGGCAACA GGATGCAGAA 
ATAGAAGACA ATACCAATGG GTCCCCGGCC AGTGAGGACA CCCCGGAGGA GGAAGAAGAA 
GAGGAGGAGG AGGAGGAGCC GGCCAGCCCA CCAGAGAGGA AGACTCTGCC CCAGATCTGC 
CTGCTCAGTA ACCCCCACTC AAGGTTCAAC CTCTGGCAGG ATCTTCCCGA GATCCGGAGC 
AGCGGGGTGC TTGAGATCCT ACAGCCCGAG GAGATTAAGC TGCAGGAGGC CATGTTCGAG 
CTGGTCACTT CCGAGGCGTC CTACTACAAG AGTCTGAACC TGCTCGTGTC CCACTTCATG 
GAGAACGAGC GGATAAGGAA GATCCTGCAC CCGTCCGAGG CGCACATCCT CTTCTCCAAC 
GTCCTGGACG TGCTGGCTGT CAGTGAGCGG TTCCTCCTGG AGCTGGAGCA CCGGATGGAG 
GAGAACATCG TCATCTCTGA CGTGTGTGAC ATTGTGTACC GTTATGCGGC CGACCACTTC 
TCTGTCTACA TCACCTACGT CAGCAATCAG ACCTACCAGG AGCGGACCTA TAAGCAGCTG 
CTCCAGGAGA AGGCAGCTTT CCGGGAGCTG ATCGCGCAGC TAGAGCTCGA CCCCAAGTGC 
AGGGGGCTGC CCTTCTCCTC CTTCCTCATC CTGCCTTTCC AGAGGATCAC ACGCCTCAAG 
CTGTTGGTCC AGAACATCCT GAAGAGGGTA GAAGAGAGGT CTGAGCGGGA GTGCACTGCT 
TTGGATGCTC ACAAGGAGCT GGAAATGGTG GTGAAGGCAT GCAACGAGGG CGTCAGGAAA 
ATGAGCCGCA CGGAACAGAT GATCAGCATT CAGAAGAAGA TGGAGTTCAA GATCAAGTCG 
GTGCCCATCA TCTCCCACTC CCGCTGGCTG CTGAAGCAGG GTGAGCTGCA GCAGATGTCA 
GGCCCCAAGA CCTCCCGGAC CCTGAGGACC AAGAAGCTCT TCCACGAAAT TTACCTCTTC 
CTGTTCAACG ACCTGCTGGT GATCTGCCGG CAGATTCCAG GAGACAAGTA CCAGGTATTT 
GACTCAGCTC CGCGGGGACT GCTGCGTGTG GAGGAGCTGG AGGACCAGGG CCAGACGCTG 
GCCAACGTGT TCATCCTGCG GCTGCTGGAG AACGCAGATG ACCGGGAGGC CACCTACATG 
CTAAAGGCGT CCTCTCAGAG TGAGATGAAG CGTTGGATGA CCTCACTGGC CCCCAACAGG 
AGGACCAAGT TTGTTTCGTT CACATCCCGG CTGCTGGACT GCCCCCAGGT CCAGTGCGTG 
CACCCATACG TGGCTCAGCA GCCAGACGAG CTGACGCTGG AGCTCGCCGA CATCCTCAAC 
ATCCTGGACA AGACTGACGA CGGGTGGATC TTTGGCGAGC GTCTGCACGA CCAGGAGAGA 
GGCTGGTTCC CCAGCTCCAT GACTGAGGAG ATCTTGAATC CCAAGATCCG GTCCCAGAAC 
CTCAAGGAAT GTTTCCGTGT CCACAAGATG GATGACCCTC AGCGCAGCCA GAACAAGGAC 
CGCAGGAAGC TGGGCAGCCG GAATCGGCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509225.1
CDS	216..2348
Translation	METRESEDLEKTRRKSASDQWNTDNEPAKVKPELLPEKEETSQADQDIQDKEPHCHIPIKRNSIFNRSIRRKSKAKARDNPERNASCLADSQDNGKSVNEPLTLNIPWSRMPPCRTAMQTDPGAQEMSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	XM_011510923.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neuronal guanine nucleotide exchange factor (NGEF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510923.3

ORF Insert Sequence:

ATGGAGACCA GGGAATCTGA AGATTTGGAA AAGACCCGGA GGAAATCAGC AAGTGATCAA 
TGGAACACTG ATAATGAACC AGCCAAGGTG AAACCTGAGT TACTCCCAGA AAAAGAGGAG 
ACTTCTCAAG CTGACCAGGA TATCCAAGAC AAAGAGCCTC ATTGCCACAT CCCAATTAAG 
AGAAATTCCA TCTTCAATCG CTCCATAAGA CGCAAAAGCA AAGCCAAGGC CAGAGACAAC 
CCCGAACGGA ACGCCAGCTG CCTGGCAGAT TCACAGGACA ATGGAAAATC TGTAAATGAG 
CCCCTGACCT TGAATATCCC CTGGAGCAGA ATGCCTCCTT GCAGAACAGC AATGCAGACA 
GACCCAGGAG CCCAGGAAAT GAGTGAGTCG TCCTCCACCC CGGGAAATGG GGCCACGCCC 
GAGGAGTGGC CGGCCCTGGC CGACAGCCCC ACCACGCTCA CCGAGGCCCT GCGGATGATC 
CACCCCATTC CCGCCGACTC CTGGAGAAAC CTCATTGAAC AAATAGGGCT CCTGTATCAG 
GAATACCGAG ATAAATCGAC TCTCCAAGAA ATCGAAACCA GGAGGCAACA GGATGCAGAA 
ATAGAAGACA ATACCAATGG GTCCCCGGCC AGTGAGGACA CCCCGGAGGA GGAAGAAGAA 
GAGGAGGAGG AGGAGGAGCC GGCCAGCCCA CCAGAGAGGA AGACTCTGCC CCAGATCTGC 
CTGCTCAGTA ACCCCCACTC AAGGTTCAAC CTCTGGCAGG ATCTTCCCGA GATCCGGAGC 
AGCGGGGTGC TTGAGATCCT ACAGCCCGAG GAGATTAAGC TGCAGGAGGC CATGTTCGAG 
CTGGTCACTT CCGAGGCGTC CTACTACAAG AGTCTGAACC TGCTCGTGTC CCACTTCATG 
GAGAACGAGC GGATAAGGAA GATCCTGCAC CCGTCCGAGG CGCACATCCT CTTCTCCAAC 
GTCCTGGACG TGCTGGCTGT CAGTGAGCGG TTCCTCCTGG AGCTGGAGCA CCGGATGGAG 
GAGAACATCG TCATCTCTGA CGTGTGTGAC ATTGTGTACC GTTATGCGGC CGACCACTTC 
TCTGTCTACA TCACCTACGT CAGCAATCAG ACCTACCAGG AGCGGACCTA TAAGCAGCTG 
CTCCAGGAGA AGGCAGCTTT CCGGGAGCTG ATCGCGCAGC TAGAGCTCGA CCCCAAGTGC 
AGGGGGCTGC CCTTCTCCTC CTTCCTCATC CTGCCTTTCC AGAGGATCAC ACGCCTCAAG 
CTGTTGGTCC AGAACATCCT GAAGAGGGTA GAAGAGAGGT CTGAGCGGGA GTGCACTGCT 
TTGGATGCTC ACAAGGAGCT GGAAATGGTG GTGAAGGCAT GCAACGAGGG CGTCAGGAAA 
ATGAGCCGCA CGGAACAGAT GATCAGCATT CAGAAGAAGA TGGAGTTCAA GATCAAGTCG 
GTGCCCATCA TCTCCCACTC CCGCTGGCTG CTGAAGCAGG GTGAGCTGCA GCAGATGTCA 
GGCCCCAAGA CCTCCCGGAC CCTGAGGACC AAGAAGCTCT TCCACGAAAT TTACCTCTTC 
CTGTTCAACG ACCTGCTGGT GATCTGCCGG CAGATTCCAG GAGACAAGTA CCAGGTATTT 
GACTCAGCTC CGCGGGGACT GCTGCGTGTG GAGGAGCTGG AGGACCAGGG CCAGACGCTG 
GCCAACGTGT TCATCCTGCG GCTGCTGGAG AACGCAGATG ACCGGGAGGC CACCTACATG 
CTAAAGGCGT CCTCTCAGAG TGAGATGAAG CGTTGGATGA CCTCACTGGC CCCCAACAGG 
AGGACCAAGT TTGTTTCGTT CACATCCCGG CTGCTGGACT GCCCCCAGGT CCAGTGCGTG 
CACCCATACG TGGCTCAGCA GCCAGACGAG CTGACGCTGG AGCTCGCCGA CATCCTCAAC 
ATCCTGGACA AGACTGACGA CGGGTGGATC TTTGGCGAGC GTCTGCACGA CCAGGAGAGA 
GGCTGGTTCC CCAGCTCCAT GACTGAGGAG ATCTTGAATC CCAAGATCCG GTCCCAGAAC 
CTCAAGGAAT GTTTCCGTGT CCACAAGATG GATGACCCTC AGCGCAGCCA GAACAAGGAC 
CGCAGGAAGC TGGGCAGCCG GAATCGGCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Common Variant of NGEF Is Associated with Abdominal Visceral Fat in Korean Men.
PloS one10(9)e0137564(2015)
Kim HJ,Park JH,Lee S,Son HY,Hwang J,Chae J,Yun JM,Kwon H,Kim JI,Cho B

Biological insights from 108 schizophrenia-associated genetic loci.
Nature511(7510)421-7(2014 Jul)

Variants in the 1q21 risk region are associated with a visual endophenotype of autism and schizophrenia.
Genes, brain, and behavior13(2)144-51(2014 Feb)
Goodbourn PT,Bosten JM,Bargary G,Hogg RE,Lawrance-Owen AJ,Mollon JD

Genome-wide association analysis identifies 13 new risk loci for schizophrenia.
Nature genetics45(10)1150-9(2013 Oct)
Ripke S,O'Dushlaine C,Chambert K,Moran JL,K?hler AK,Akterin S,Bergen SE,Collins AL,Crowley JJ,Fromer M,Kim Y,Lee SH,Magnusson PK,Sanchez N,Stahl EA,Williams S,Wray NR,Xia K,Bettella F,Borglum AD,Bulik-Sullivan BK,Cormican P,Craddock N,de Leeuw C,Durmishi N,Gill M,Golimbet V,Hamshere ML,Holmans P,Hougaard DM,Kendler KS,Lin K,Morris DW,Mors O,Mortensen PB,Neale BM,O'Neill FA,Owen MJ,Milovancevic MP,Posthuma D,Powell J,Richards AL,Riley BP,Ruderfer D,Rujescu D,Sigurdsson E,Silagadze T,Smit AB,Stefansson H,Steinberg S,Suvisaari J,Tosato S,Verhage M,Walters JT,,Levinson DF,Gejman PV,Kendler KS,Laurent C,Mowry BJ,O'Donovan MC,Owen MJ,Pulver AE,Riley BP,Schwab SG,Wildenauer DB,Dudbridge F,Holmans P,Shi J,Albus M,Alexander M,Campion D,Cohen D,Dikeos D,Duan J,Eichhammer P,Godard S,Hansen M,Lerer FB,Liang KY,Maier W,Mallet J,Nertney DA,Nestadt G,Norton N,O'Neill FA,Papadimitriou GN,Ribble R,Sanders AR,Silverman JM,Walsh D,Williams NM,Wormley B,,Arranz MJ,Bakker S,Bender S,Bramon E,Collier D,Crespo-Facorro B,Hall J,Iyegbe C,Jablensky A,Kahn RS,Kalaydjieva L,Lawrie S,Lewis CM,Lin K,Linszen DH,Mata I,McIntosh A,Murray RM,Ophoff RA,Powell J,Rujescu D,Van Os J,Walshe M,Weisbrod M,Wiersma D,,Donnelly P,Barroso I,Blackwell JM,Bramon E,Brown MA,Casas JP,Corvin AP,Deloukas P,Duncanson A,Jankowski J,Markus HS,Mathew CG,Palmer CN,Plomin R,Rautanen A,Sawcer SJ,Trembath RC,Viswanathan AC,Wood NW,Spencer CC,Band G,Bellenguez C,Freeman C,Hellenthal G,Giannoulatou E,Pirinen M,Pearson RD,Strange A,Su Z,Vukcevic D,Donnelly P,Langford C,Hunt SE,Edkins S,Gwilliam R,Blackburn H,Bumpstead SJ,Dronov S,Gillman M,Gray E,Hammond N,Jayakumar A,McCann OT,Liddle J,Potter SC,Ravindrarajah R,Ricketts M,Tashakkori-Ghanbaria A,Waller MJ,Weston P,Widaa S,Whittaker P,Barroso I,Deloukas P,Mathew CG,Blackwell JM,Brown MA,Corvin AP,McCarthy MI,Spencer CC,Bramon E,Corvin AP,O'Donovan MC,Stefansson K,Scolnick E,Purcell S,McCarroll SA,Sklar P,Hultman CM,Sullivan PF

Identification and analysis of intermediate size noncoding RNAs in the human fetal brain.
PloS one6(7)e21652(2011)
Yan D,He D,He S,Chen X,Fan Z,Chen R

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NGEF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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