Atp2a3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp2a3
Entrez Gene ID	53313
Full Name	ATPase, Ca++ transporting, ubiquitous
Synonyms	SERCA3b,Serca3
General protein information	Preferred Names ATPase, Ca++ transporting, ubiquitous Names sarcoplasmic/endoplasmic reticulum calcium ATPase 3 SR Ca(2+)-ATPase 3 calcium pump 3 sarco /endoplasmic reticulum Ca2+ -ATPase sarcoendoplasmic reticulum Ca2+ ATPase type 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001163337.1	NP_001156809.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 3 isoform c
NM_001163336.1	NP_001156808.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 3 isoform a
NM_016745.3	NP_058025.2	sarcoplasmic/endoplasmic reticulum calcium ATPase 3 isoform b

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP2A3	XP_548558.3
Danio rerio (zebrafish)	atp2a3	XP_697108.5
Homo sapiens (human)	ATP2A3	NP_777615.1
Bos taurus (cattle)	ATP2A3	NP_001107626.1
Pan troglodytes (chimpanzee)	ATP2A3	XP_003952991.1
Xenopus tropicalis (tropical clawed frog)	atp2a3	NP_001072333.1
Mus musculus (house mouse)	Atp2a3	NP_058025.2
	ATP2A3	XP_002800297.1
Rattus norvegicus (Norway rat)	Atp2a3	NP_037046.1
Gallus gallus (chicken)	ATP2A3	NP_990222.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread control of calcium signaling by a family of SERCA-inhibiting micropeptides.
Anderson DM, Makarewich CA, Anderson KM, Shelton JM, Bezprozvannaya S, Bassel-Duby R, Olson EN
Science signaling9(457)ra119(2016 Dec)

Full activation of mouse platelets requires ADP secretion regulated by SERCA3 ATPase-dependent calcium stores.
Ela?b Z, Adam F, Berrou E, Bordet JC, Pr?vost N, Bobe R, Bryckaert M, Rosa JP
Blood128(8)1129-38(2016 08)

A micropeptide encoded by a putative long noncoding RNA regulates muscle performance.
Anderson DM, Anderson KM, Chang CL, Makarewich CA, Nelson BR, McAnally JR, Kasaragod P, Shelton JM, Liou J, Bassel-Duby R, Olson EN
Cell160(4)595-606(2015 Feb)

Atrial natriuretic peptide locally counteracts the deleterious effects of cardiomyocyte mineralocorticoid receptor activation.
Nakagawa H, Oberwinkler H, Nikolaev VO, Ga?ner B, Umbenhauer S, Wagner H, Saito Y, Baba HA, Frantz S, Kuhn M
Circulation. Heart failure7(5)814-21(2014 Sep)

Placental PPAR? regulates spatiotemporally diverse genes and a unique metabolic network.
Shalom-Barak T, Zhang X, Chu T, Timothy Schaiff W, Reddy JK, Xu J, Sadovsky Y, Barak Y
Developmental biology372(1)143-55(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SERCA3-dependent Ca(2+) stores control a specific ADP secretion pathway.
Title: Full activation of mouse platelets requires ADP secretion regulated by SERCA3 ATPase-dependent calcium stores.

ANP counter-regulates cardiac MR activation in hypertensive heart disease. An imbalance in cardiac ANP/GC-A (inhibition) and aldosterone/MR signaling (augmentation) favors adverse cardiac remodeling in chronic pressure overload.
Title: Atrial natriuretic peptide locally counteracts the deleterious effects of cardiomyocyte mineralocorticoid receptor activation.

This study evaluated the role of SERCA2b and SERCA3 in the control of the free calcium concentration in the endoplasmic reticulum and the role of SERCA3 in the control of insulin secretion.
Title: Mechanisms of control of the free Ca2+ concentration in the endoplasmic reticulum of mouse pancreatic ?-cells: interplay with cell metabolism and [Ca2+]c and role of SERCA2b and SERCA3.

IRS-1 modulation of insulin secretion is associated with Ca(2+) signaling and expression of SERCA-2b and -3 genes in pancreatic islets. Direct link between insulin resistance and defective insulin secretion.
Title: Islet secretory defect in insulin receptor substrate 1 null mice is linked with reduced calcium signaling and expression of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA)-2b and -3.

regulation in insulin-secreting beta-cells by insulin receptor substrate 1
Title: Insulin receptor substrate 1 regulation of sarco-endoplasmic reticulum calcium ATPase 3 in insulin-secreting beta-cells.

The following Atp2a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp2a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08910	NM_001163336.1 Latest version!	Mus musculus ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu10640	NM_016745.3 Latest version!	Mus musculus ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$615.30 $879.00
OMu08911	NM_001163337.1 Latest version!	Mus musculus ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp2a3 ( NM_001163336.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156808.1 Mus musculus ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08910
Clone ID Related Accession (Same CDS sequence)	NM_001163336.1
Accession Version	NM_001163336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 3 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK155904.1, AK089487.1, AK152999.1 and BY549777.1. Transcript Variant: This variant (1) encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026147.1, U49394.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGAGG CGCACCTGCT CTCCGCTGCC GACGTGCTGC GTCGCTTCTC GGTGACAGCC 
GAGGGCGGCT TGAGCCTGGA GCAGGTGACC GACGCGCGGG AGCGTTACGG CCCCAACGAG 
CTCCCGACCG AGGAAGGGAA ATCCCTGTGG GAGCTGGTGG TGGAGCAGTT TGAGGACCTC 
TTGGTGCGCA TCCTGCTGCT GGCAGCTCTG GTTTCCTTTG TCCTGGCCTG GTTCGAGGAG 
GGTGAGGAGA CCACAACGGC CTTCGTGGAG CCCCTGGTCA TCATGCTAAT TCTCGTGGCC 
AATGCGATCG TGGGAGTGTG GCAGGAACGC AACGCAGAGA GTGCAATTGA GGCCTTGAAG 
GAGTATGAGC CTGAGATGGG TAAGGTGATC CGGTCTGACC GCAAGGGTGT ACAGAGGATC 
CGAGCCAGGG ACATCGTCCC TGGGGACATT GTGGAAGTGG CAGTGGGAGA CAAGGTACCC 
GCTGACCTCC GTCTCATCGA GATCAAGTCC ACCACACTTC GAGTGGACCA GTCCATCCTG 
ACAGGGGAAT CCGTGTCTGT GACGAAGCAC ACAGATGCCA TTCCAGACCC CCGAGCCGTG 
AACCAGGACA AGAAGAACAT GCTATTTTCT GGCACCAATA TTGCATCAGG CAAAGCCCTG 
GGTGTGGCTG TGGCTACAGG CCTGCAGACA GAGCTGGGCA AGATTCGGAG CCAGATGGCG 
GCTGTGGAGC CTGAGCGGAC GCCATTGCAG CGCAAGCTGG ATGAATTTGG GAGGCAGCTG 
TCCCATGCCA TCTCTGTGAT CTGTGTGGCT GTGTGGGTCA TCAACATCGG CCACTTTGCT 
GACCCGGCCC ACGGTGGATC CTGGCTCCGT GGTGCAGTCT ACTACTTCAA GATCGCTGTG 
GCCCTGGCTG TGGCTGCTAT CCCCGAGGGT CTCCCAGCAG TCATCACTAC ATGCCTGGCC 
CTGGGCACAA GACGTATGGC ACGTAAGAAT GCCATTGTGC GGAGCCTGCC TTCTGTGGAG 
ACGCTGGGCT GCACCTCGGT CATCTGCTCT GACAAGACGG GGACGCTCAC CACCAATCAG 
ATGTCTGTCT GCAGGATGTT CGTGGTGGCT GAAGCAGAGG CAGGCACCTG TCGTTTGCAT 
GAATTCACCA TTTCGGGTAC CACGTACACC CCAGAGGGCG AAGTGAGGCA AGGGGAGCAG 
CCTGTGCGCT GTGGGCAGTT TGATGGGCTA GTGGAGCTCG CCACCATCTG TGCCCTCTGC 
AATGATTCTG CGCTGGACTA CAATGAGGCC AAGGGTGTGT ATGAGAAGGT AGGAGAGGCC 
ACAGAGACCG CCCTGACTTG CCTGGTAGAG AAGATGAATG TGTTTGACAC AGACCTGAAA 
GGACTGTCGC GGGTGGAGCG TGCTGGCGCC TGCAACTCAG TCATCAAGCA ACTCATGCGG 
AAAGAGTTCA CCCTGGAGTT CTCCCGGGAC CGGAAGTCTA TGTCCGTGTA CTGCACACCT 
ACTCGTGCTG ACCCCAAGGT CCAGGGCAGC AAGATGTTTG TGAAGGGAGC TCCTGAGAGT 
GTAATTGAGC GCTGTAGCTC AGTCCGAGTG GGCAGCCGAA CAGCACCCCT GAGCACCACT 
TCCAGAGAGC ATATCCTGGC CAAGATCCGG GACTGGGGCT CAGGCTCTGA CACACTGCGC 
TGCTTAGCCT TGGCCACCAG GGACACACCC CCAAGGAAGG AGGACATGCA TCTAGATGAC 
TGCAGCCGGT TTGTACAGTA CGAGACAGAC CTGACCTTCG TGGGCTGCGT GGGCATGTTA 
GACCCACCGA GACCAGAGGT GGCTGCTTGT ATCACACGCT GTTCCCGGGC TGGCATTCGA 
GTGGTCATGA TCACAGGGGA CAACAAAGGG ACAGCTGTGG CCATCTGCCG CCGACTTGGC 
ATCTTTGGGG ACACGGAGGA TGTGTTGGGC AAGGCCTACA CGGGCCGTGA GTTTGATGAC 
CTCAGCCCAG AGCAGCAGCG CCAGGCTTGT CGCACTGCCC GGTGCTTTGC CCGAGTGGAA 
CCCGCACATA AGTCTCGAAT CGTGGAGAAC CTACAGTCCT TTAATGAGAT CACTGCCATG 
ACTGGCGATG GGGTGAATGA TGCACCAGCC CTGAAGAAGG CAGAGATCGG CATCGCCATG 
GGCTCAGGCA CTGCTGTGGC CAAGTCAGCA GCAGAGATGG TGCTGTCCGA TGACAACTTT 
GCCTCCATTG TGGCTGCAGT AGAAGAGGGC AGGGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATCTCCTC CAATGTTGGC GAGGTTGTCT GCATCTTCCT CACAGCAATT 
CTGGGCCTGC CCGAAGCTCT GATCCCTGTG CAGCTGCTCT GGGTGAACCT GGTAACAGAT 
GGCTTACCGG CCACAGCCCT TGGCTTCAAC CCACCAGATC TGGATATCAT GGAGAAGCCG 
CCTCGGAATC CCCGTGAGGC CCTCATCAGT GGCTGGCTTT TTTTCCGCTA TTTGGCTATT 
GGAGTGTATG TAGGCCTGGC TACAGTGGCT GCCGCCACCT GGTGGTTCCT GTATGACACC 
GAGGGACCTC AAGTCACCTT CTATCAGCTG AGGAACTTCC TGAAGTGCTC TGAAGACAAT 
CCACTGTTTG CTGGCATTGA CTGCAAGGTT TTTGAGTCAC GCTTCCCCAC AACCATGGCC 
TTATCTGTGC TTGTGACCAT TGAAATGTGC AACGCCCTCA ACAGCGTCTC TGAGAACCAG 
TCACTGCTGC GCATGCCACC CTGGCTGAAC CCTTGGCTGC TGGGGGCTGT GGTCATGTCC 
ATGGCTCTGC ATTTTCTTAT CCTCCTGGTG CCACCTCTGC CTCTCATTTT TCAGGTGACC 
CCACTGAGTG GTCGGCAGTG GGGGGTGGTG CTTCAGATGT CTCTGCCTGT CATCCTGCTC 
GATGAGGCCC TCAAGTATCT GTCCAGGAAT CACATGGATG AAAAAAAGGA CCTGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156808.1
CDS	186..3185
Translation	MEEAHLLSAADVLRRFSVTAEGGLSLEQVTDARERYGPNELPTEEGKSLWELVVEQFEDLLVRILLLAALVSFVLAWFEEGEETTTAFVEPLVIMLILVANAIVGVWQERNAESAIEALKEYEPEMGKVIRSDRKGVQRIRARDIVPGDIVEVAVGDKVPADLRLIEIKSTTLRVDQSILTGESVSVTKHTDAIPDPRAVNQDKKNMLFSGTNIASGKALGVAVATGLQTELGKIRSQMAAVEPERTPLQRKLDEFGRQLSHAISVICVAVWVINIGHFADPAHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFVVAEAEAGTCRLHEFTISGTTYTPEGEVRQGEQPVRCGQFDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLKGLSRVERAGACNSVIKQLMRKEFTLEFSRDRKSMSVYCTPTRADPKVQGSKMFVKGAPESVIERCSSVRVGSRTAPLSTTSREHILAKIRDWGSGSDTLRCLALATRDTPPRKEDMHLDDCSRFVQYETDLTFVGCVGMLDPPRPEVAACITRCSRAGIRVVMITGDNKGTAVAICRRLGIFGDTEDVLGKAYTGREFDDLSPEQQRQACRTARCFARVEPAHKSRIVENLQSFNEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEKPPRNPREALISGWLFFRYLAIGVYVGLATVAAATWWFLYDTEGPQVTFYQLRNFLKCSEDNPLFAGIDCKVFESRFPTTMALSVLVTIEMCNALNSVSENQSLLRMPPWLNPWLLGAVVMSMALHFLILLVPPLPLIFQVTPLSGRQWGVVLQMSLPVILLDEALKYLSRNHMDEKKDLK

Target ORF information:

RefSeq Version	NM_001163336.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163336.1

ORF Insert Sequence:

ATGGAGGAGG CGCACCTGCT CTCCGCTGCC GACGTGCTGC GTCGCTTCTC GGTGACAGCC 
GAGGGCGGCT TGAGCCTGGA GCAGGTGACC GACGCGCGGG AGCGTTACGG CCCCAACGAG 
CTCCCGACCG AGGAAGGGAA ATCCCTGTGG GAGCTGGTGG TGGAGCAGTT TGAGGACCTC 
TTGGTGCGCA TCCTGCTGCT GGCAGCTCTG GTTTCCTTTG TCCTGGCCTG GTTCGAGGAG 
GGTGAGGAGA CCACAACGGC CTTCGTGGAG CCCCTGGTCA TCATGCTAAT TCTCGTGGCC 
AATGCGATCG TGGGAGTGTG GCAGGAACGC AACGCAGAGA GTGCAATTGA GGCCTTGAAG 
GAGTATGAGC CTGAGATGGG TAAGGTGATC CGGTCTGACC GCAAGGGTGT ACAGAGGATC 
CGAGCCAGGG ACATCGTCCC TGGGGACATT GTGGAAGTGG CAGTGGGAGA CAAGGTACCC 
GCTGACCTCC GTCTCATCGA GATCAAGTCC ACCACACTTC GAGTGGACCA GTCCATCCTG 
ACAGGGGAAT CCGTGTCTGT GACGAAGCAC ACAGATGCCA TTCCAGACCC CCGAGCCGTG 
AACCAGGACA AGAAGAACAT GCTATTTTCT GGCACCAATA TTGCATCAGG CAAAGCCCTG 
GGTGTGGCTG TGGCTACAGG CCTGCAGACA GAGCTGGGCA AGATTCGGAG CCAGATGGCG 
GCTGTGGAGC CTGAGCGGAC GCCATTGCAG CGCAAGCTGG ATGAATTTGG GAGGCAGCTG 
TCCCATGCCA TCTCTGTGAT CTGTGTGGCT GTGTGGGTCA TCAACATCGG CCACTTTGCT 
GACCCGGCCC ACGGTGGATC CTGGCTCCGT GGTGCAGTCT ACTACTTCAA GATCGCTGTG 
GCCCTGGCTG TGGCTGCTAT CCCCGAGGGT CTCCCAGCAG TCATCACTAC ATGCCTGGCC 
CTGGGCACAA GACGTATGGC ACGTAAGAAT GCCATTGTGC GGAGCCTGCC TTCTGTGGAG 
ACGCTGGGCT GCACCTCGGT CATCTGCTCT GACAAGACGG GGACGCTCAC CACCAATCAG 
ATGTCTGTCT GCAGGATGTT CGTGGTGGCT GAAGCAGAGG CAGGCACCTG TCGTTTGCAT 
GAATTCACCA TTTCGGGTAC CACGTACACC CCAGAGGGCG AAGTGAGGCA AGGGGAGCAG 
CCTGTGCGCT GTGGGCAGTT TGATGGGCTA GTGGAGCTCG CCACCATCTG TGCCCTCTGC 
AATGATTCTG CGCTGGACTA CAATGAGGCC AAGGGTGTGT ATGAGAAGGT AGGAGAGGCC 
ACAGAGACCG CCCTGACTTG CCTGGTAGAG AAGATGAATG TGTTTGACAC AGACCTGAAA 
GGACTGTCGC GGGTGGAGCG TGCTGGCGCC TGCAACTCAG TCATCAAGCA ACTCATGCGG 
AAAGAGTTCA CCCTGGAGTT CTCCCGGGAC CGGAAGTCTA TGTCCGTGTA CTGCACACCT 
ACTCGTGCTG ACCCCAAGGT CCAGGGCAGC AAGATGTTTG TGAAGGGAGC TCCTGAGAGT 
GTAATTGAGC GCTGTAGCTC AGTCCGAGTG GGCAGCCGAA CAGCACCCCT GAGCACCACT 
TCCAGAGAGC ATATCCTGGC CAAGATCCGG GACTGGGGCT CAGGCTCTGA CACACTGCGC 
TGCTTAGCCT TGGCCACCAG GGACACACCC CCAAGGAAGG AGGACATGCA TCTAGATGAC 
TGCAGCCGGT TTGTACAGTA CGAGACAGAC CTGACCTTCG TGGGCTGCGT GGGCATGTTA 
GACCCACCGA GACCAGAGGT GGCTGCTTGT ATCACACGCT GTTCCCGGGC TGGCATTCGA 
GTGGTCATGA TCACAGGGGA CAACAAAGGG ACAGCTGTGG CCATCTGCCG CCGACTTGGC 
ATCTTTGGGG ACACGGAGGA TGTGTTGGGC AAGGCCTACA CGGGCCGTGA GTTTGATGAC 
CTCAGCCCAG AGCAGCAGCG CCAGGCTTGT CGCACTGCCC GGTGCTTTGC CCGAGTGGAA 
CCCGCACATA AGTCTCGAAT CGTGGAGAAC CTACAGTCCT TTAATGAGAT CACTGCCATG 
ACTGGCGATG GGGTGAATGA TGCACCAGCC CTGAAGAAGG CAGAGATCGG CATCGCCATG 
GGCTCAGGCA CTGCTGTGGC CAAGTCAGCA GCAGAGATGG TGCTGTCCGA TGACAACTTT 
GCCTCCATTG TGGCTGCAGT AGAAGAGGGC AGGGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATCTCCTC CAATGTTGGC GAGGTTGTCT GCATCTTCCT CACAGCAATT 
CTGGGCCTGC CCGAAGCTCT GATCCCTGTG CAGCTGCTCT GGGTGAACCT GGTAACAGAT 
GGCTTACCGG CCACAGCCCT TGGCTTCAAC CCACCAGATC TGGATATCAT GGAGAAGCCG 
CCTCGGAATC CCCGTGAGGC CCTCATCAGT GGCTGGCTTT TTTTCCGCTA TTTGGCTATT 
GGAGTGTATG TAGGCCTGGC TACAGTGGCT GCCGCCACCT GGTGGTTCCT GTATGACACC 
GAGGGACCTC AAGTCACCTT CTATCAGCTG AGGAACTTCC TGAAGTGCTC TGAAGACAAT 
CCACTGTTTG CTGGCATTGA CTGCAAGGTT TTTGAGTCAC GCTTCCCCAC AACCATGGCC 
TTATCTGTGC TTGTGACCAT TGAAATGTGC AACGCCCTCA ACAGCGTCTC TGAGAACCAG 
TCACTGCTGC GCATGCCACC CTGGCTGAAC CCTTGGCTGC TGGGGGCTGT GGTCATGTCC 
ATGGCTCTGC ATTTTCTTAT CCTCCTGGTG CCACCTCTGC CTCTCATTTT TCAGGTGACC 
CCACTGAGTG GTCGGCAGTG GGGGGTGGTG CTTCAGATGT CTCTGCCTGT CATCCTGCTC 
GATGAGGCCC TCAAGTATCT GTCCAGGAAT CACATGGATG AAAAAAAGGA CCTGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2a3 ( NM_016745.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058025.2 Mus musculus ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu10640
Clone ID Related Accession (Same CDS sequence)	NM_016745.3
Accession Version	NM_016745.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 3 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK155904.1, AK089487.1, U49393.1, AK152999.1 and BY549777.1. On Jul 17, 2009 this sequence version replaced NM_016745.2. Transcript Variant: This variant (2) includes an additional exon in the 3' coding region resulting in a frameshift, compared to variant 1. Variant 2 encodes isoform b, which is longer and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017639.1, U49393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGAGG CGCACCTGCT CTCCGCTGCC GACGTGCTGC GTCGCTTCTC GGTGACAGCC 
GAGGGCGGCT TGAGCCTGGA GCAGGTGACC GACGCGCGGG AGCGTTACGG CCCCAACGAG 
CTCCCGACCG AGGAAGGGAA ATCCCTGTGG GAGCTGGTGG TGGAGCAGTT TGAGGACCTC 
TTGGTGCGCA TCCTGCTGCT GGCAGCTCTG GTTTCCTTTG TCCTGGCCTG GTTCGAGGAG 
GGTGAGGAGA CCACAACGGC CTTCGTGGAG CCCCTGGTCA TCATGCTAAT TCTCGTGGCC 
AATGCGATCG TGGGAGTGTG GCAGGAACGC AACGCAGAGA GTGCAATTGA GGCCTTGAAG 
GAGTATGAGC CTGAGATGGG TAAGGTGATC CGGTCTGACC GCAAGGGTGT ACAGAGGATC 
CGAGCCAGGG ACATCGTCCC TGGGGACATT GTGGAAGTGG CAGTGGGAGA CAAGGTACCC 
GCTGACCTCC GTCTCATCGA GATCAAGTCC ACCACACTTC GAGTGGACCA GTCCATCCTG 
ACAGGGGAAT CCGTGTCTGT GACGAAGCAC ACAGATGCCA TTCCAGACCC CCGAGCCGTG 
AACCAGGACA AGAAGAACAT GCTATTTTCT GGCACCAATA TTGCATCAGG CAAAGCCCTG 
GGTGTGGCTG TGGCTACAGG CCTGCAGACA GAGCTGGGCA AGATTCGGAG CCAGATGGCG 
GCTGTGGAGC CTGAGCGGAC GCCATTGCAG CGCAAGCTGG ATGAATTTGG GAGGCAGCTG 
TCCCATGCCA TCTCTGTGAT CTGTGTGGCT GTGTGGGTCA TCAACATCGG CCACTTTGCT 
GACCCGGCCC ACGGTGGATC CTGGCTCCGT GGTGCAGTCT ACTACTTCAA GATCGCTGTG 
GCCCTGGCTG TGGCTGCTAT CCCCGAGGGT CTCCCAGCAG TCATCACTAC ATGCCTGGCC 
CTGGGCACAA GACGTATGGC ACGTAAGAAT GCCATTGTGC GGAGCCTGCC TTCTGTGGAG 
ACGCTGGGCT GCACCTCGGT CATCTGCTCT GACAAGACGG GGACGCTCAC CACCAATCAG 
ATGTCTGTCT GCAGGATGTT CGTGGTGGCT GAAGCAGAGG CAGGCACCTG TCGTTTGCAT 
GAATTCACCA TTTCGGGTAC CACGTACACC CCAGAGGGCG AAGTGAGGCA AGGGGAGCAG 
CCTGTGCGCT GTGGGCAGTT TGATGGGCTA GTGGAGCTCG CCACCATCTG TGCCCTCTGC 
AATGATTCTG CGCTGGACTA CAATGAGGCC AAGGGTGTGT ATGAGAAGGT AGGAGAGGCC 
ACAGAGACCG CCCTGACTTG CCTGGTAGAG AAGATGAATG TGTTTGACAC AGACCTGAAA 
GGACTGTCGC GGGTGGAGCG TGCTGGCGCC TGCAACTCAG TCATCAAGCA ACTCATGCGG 
AAAGAGTTCA CCCTGGAGTT CTCCCGGGAC CGGAAGTCTA TGTCCGTGTA CTGCACACCT 
ACTCGTGCTG ACCCCAAGGT CCAGGGCAGC AAGATGTTTG TGAAGGGAGC TCCTGAGAGT 
GTAATTGAGC GCTGTAGCTC AGTCCGAGTG GGCAGCCGAA CAGCACCCCT GAGCACCACT 
TCCAGAGAGC ATATCCTGGC CAAGATCCGG GACTGGGGCT CAGGCTCTGA CACACTGCGC 
TGCTTAGCCT TGGCCACCAG GGACACACCC CCAAGGAAGG AGGACATGCA TCTAGATGAC 
TGCAGCCGGT TTGTACAGTA CGAGACAGAC CTGACCTTCG TGGGCTGCGT GGGCATGTTA 
GACCCACCGA GACCAGAGGT GGCTGCTTGT ATCACACGCT GTTCCCGGGC TGGCATTCGA 
GTGGTCATGA TCACAGGGGA CAACAAAGGG ACAGCTGTGG CCATCTGCCG CCGACTTGGC 
ATCTTTGGGG ACACGGAGGA TGTGTTGGGC AAGGCCTACA CGGGCCGTGA GTTTGATGAC 
CTCAGCCCAG AGCAGCAGCG CCAGGCTTGT CGCACTGCCC GGTGCTTTGC CCGAGTGGAA 
CCCGCACATA AGTCTCGAAT CGTGGAGAAC CTACAGTCCT TTAATGAGAT CACTGCCATG 
ACTGGCGATG GGGTGAATGA TGCACCAGCC CTGAAGAAGG CAGAGATCGG CATCGCCATG 
GGCTCAGGCA CTGCTGTGGC CAAGTCAGCA GCAGAGATGG TGCTGTCCGA TGACAACTTT 
GCCTCCATTG TGGCTGCAGT AGAAGAGGGC AGGGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATCTCCTC CAATGTTGGC GAGGTTGTCT GCATCTTCCT CACAGCAATT 
CTGGGCCTGC CCGAAGCTCT GATCCCTGTG CAGCTGCTCT GGGTGAACCT GGTAACAGAT 
GGCTTACCGG CCACAGCCCT TGGCTTCAAC CCACCAGATC TGGATATCAT GGAGAAGCCG 
CCTCGGAATC CCCGTGAGGC CCTCATCAGT GGCTGGCTTT TTTTCCGCTA TTTGGCTATT 
GGAGTGTATG TAGGCCTGGC TACAGTGGCT GCCGCCACCT GGTGGTTCCT GTATGACACC 
GAGGGACCTC AAGTCACCTT CTATCAGCTG AGGAACTTCC TGAAGTGCTC TGAAGACAAT 
CCACTGTTTG CTGGCATTGA CTGCAAGGTT TTTGAGTCAC GCTTCCCCAC AACCATGGCC 
TTATCTGTGC TTGTGACCAT TGAAATGTGC AACGCCCTCA ACAGCGTCTC TGAGAACCAG 
TCACTGCTGC GCATGCCACC CTGGCTGAAC CCTTGGCTGC TGGGGGCTGT GGTCATGTCC 
ATGGCTCTGC ATTTTCTTAT CCTCCTGGTG CCACCTCTGC CTCTCATTTT TCAGGTGACC 
CCACTGAGTG GTCGGCAGTG GGGGGTGGTG CTTCAGATGT CTCTGCCTGT CATCCTGCTC 
GATGAGGCCC TCAAGTATCT GTCCAGGAAT CACATGGATG GTGTTCTCGG GACATTTATG 
CAGGCTCGGA GTAGGCAGCT GCCGACTACT TCCAGGACCC CATACCACAC CGGAAAAAAA 
GGACCTGAAG TAAATCCTGG GAGCAGAGGG GAGTCTCCAG TGTGGCCCTC AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058025.2
CDS	186..3302
Translation	MEEAHLLSAADVLRRFSVTAEGGLSLEQVTDARERYGPNELPTEEGKSLWELVVEQFEDLLVRILLLAALVSFVLAWFEEGEETTTAFVEPLVIMLILVANAIVGVWQERNAESAIEALKEYEPEMGKVIRSDRKGVQRIRARDIVPGDIVEVAVGDKVPADLRLIEIKSTTLRVDQSILTGESVSVTKHTDAIPDPRAVNQDKKNMLFSGTNIASGKALGVAVATGLQTELGKIRSQMAAVEPERTPLQRKLDEFGRQLSHAISVICVAVWVINIGHFADPAHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFVVAEAEAGTCRLHEFTISGTTYTPEGEVRQGEQPVRCGQFDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLKGLSRVERAGACNSVIKQLMRKEFTLEFSRDRKSMSVYCTPTRADPKVQGSKMFVKGAPESVIERCSSVRVGSRTAPLSTTSREHILAKIRDWGSGSDTLRCLALATRDTPPRKEDMHLDDCSRFVQYETDLTFVGCVGMLDPPRPEVAACITRCSRAGIRVVMITGDNKGTAVAICRRLGIFGDTEDVLGKAYTGREFDDLSPEQQRQACRTARCFARVEPAHKSRIVENLQSFNEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEKPPRNPREALISGWLFFRYLAIGVYVGLATVAAATWWFLYDTEGPQVTFYQLRNFLKCSEDNPLFAGIDCKVFESRFPTTMALSVLVTIEMCNALNSVSENQSLLRMPPWLNPWLLGAVVMSMALHFLILLVPPLPLIFQVTPLSGRQWGVVLQMSLPVILLDEALKYLSRNHMDGVLGTFMQARSRQLPTTSRTPYHTGKKGPEVNPGSRGESPVWPSD

Target ORF information:

RefSeq Version	NM_016745.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016745.3

ORF Insert Sequence:

ATGGAGGAGG CGCACCTGCT CTCCGCTGCC GACGTGCTGC GTCGCTTCTC GGTGACAGCC 
GAGGGCGGCT TGAGCCTGGA GCAGGTGACC GACGCGCGGG AGCGTTACGG CCCCAACGAG 
CTCCCGACCG AGGAAGGGAA ATCCCTGTGG GAGCTGGTGG TGGAGCAGTT TGAGGACCTC 
TTGGTGCGCA TCCTGCTGCT GGCAGCTCTG GTTTCCTTTG TCCTGGCCTG GTTCGAGGAG 
GGTGAGGAGA CCACAACGGC CTTCGTGGAG CCCCTGGTCA TCATGCTAAT TCTCGTGGCC 
AATGCGATCG TGGGAGTGTG GCAGGAACGC AACGCAGAGA GTGCAATTGA GGCCTTGAAG 
GAGTATGAGC CTGAGATGGG TAAGGTGATC CGGTCTGACC GCAAGGGTGT ACAGAGGATC 
CGAGCCAGGG ACATCGTCCC TGGGGACATT GTGGAAGTGG CAGTGGGAGA CAAGGTACCC 
GCTGACCTCC GTCTCATCGA GATCAAGTCC ACCACACTTC GAGTGGACCA GTCCATCCTG 
ACAGGGGAAT CCGTGTCTGT GACGAAGCAC ACAGATGCCA TTCCAGACCC CCGAGCCGTG 
AACCAGGACA AGAAGAACAT GCTATTTTCT GGCACCAATA TTGCATCAGG CAAAGCCCTG 
GGTGTGGCTG TGGCTACAGG CCTGCAGACA GAGCTGGGCA AGATTCGGAG CCAGATGGCG 
GCTGTGGAGC CTGAGCGGAC GCCATTGCAG CGCAAGCTGG ATGAATTTGG GAGGCAGCTG 
TCCCATGCCA TCTCTGTGAT CTGTGTGGCT GTGTGGGTCA TCAACATCGG CCACTTTGCT 
GACCCGGCCC ACGGTGGATC CTGGCTCCGT GGTGCAGTCT ACTACTTCAA GATCGCTGTG 
GCCCTGGCTG TGGCTGCTAT CCCCGAGGGT CTCCCAGCAG TCATCACTAC ATGCCTGGCC 
CTGGGCACAA GACGTATGGC ACGTAAGAAT GCCATTGTGC GGAGCCTGCC TTCTGTGGAG 
ACGCTGGGCT GCACCTCGGT CATCTGCTCT GACAAGACGG GGACGCTCAC CACCAATCAG 
ATGTCTGTCT GCAGGATGTT CGTGGTGGCT GAAGCAGAGG CAGGCACCTG TCGTTTGCAT 
GAATTCACCA TTTCGGGTAC CACGTACACC CCAGAGGGCG AAGTGAGGCA AGGGGAGCAG 
CCTGTGCGCT GTGGGCAGTT TGATGGGCTA GTGGAGCTCG CCACCATCTG TGCCCTCTGC 
AATGATTCTG CGCTGGACTA CAATGAGGCC AAGGGTGTGT ATGAGAAGGT AGGAGAGGCC 
ACAGAGACCG CCCTGACTTG CCTGGTAGAG AAGATGAATG TGTTTGACAC AGACCTGAAA 
GGACTGTCGC GGGTGGAGCG TGCTGGCGCC TGCAACTCAG TCATCAAGCA ACTCATGCGG 
AAAGAGTTCA CCCTGGAGTT CTCCCGGGAC CGGAAGTCTA TGTCCGTGTA CTGCACACCT 
ACTCGTGCTG ACCCCAAGGT CCAGGGCAGC AAGATGTTTG TGAAGGGAGC TCCTGAGAGT 
GTAATTGAGC GCTGTAGCTC AGTCCGAGTG GGCAGCCGAA CAGCACCCCT GAGCACCACT 
TCCAGAGAGC ATATCCTGGC CAAGATCCGG GACTGGGGCT CAGGCTCTGA CACACTGCGC 
TGCTTAGCCT TGGCCACCAG GGACACACCC CCAAGGAAGG AGGACATGCA TCTAGATGAC 
TGCAGCCGGT TTGTACAGTA CGAGACAGAC CTGACCTTCG TGGGCTGCGT GGGCATGTTA 
GACCCACCGA GACCAGAGGT GGCTGCTTGT ATCACACGCT GTTCCCGGGC TGGCATTCGA 
GTGGTCATGA TCACAGGGGA CAACAAAGGG ACAGCTGTGG CCATCTGCCG CCGACTTGGC 
ATCTTTGGGG ACACGGAGGA TGTGTTGGGC AAGGCCTACA CGGGCCGTGA GTTTGATGAC 
CTCAGCCCAG AGCAGCAGCG CCAGGCTTGT CGCACTGCCC GGTGCTTTGC CCGAGTGGAA 
CCCGCACATA AGTCTCGAAT CGTGGAGAAC CTACAGTCCT TTAATGAGAT CACTGCCATG 
ACTGGCGATG GGGTGAATGA TGCACCAGCC CTGAAGAAGG CAGAGATCGG CATCGCCATG 
GGCTCAGGCA CTGCTGTGGC CAAGTCAGCA GCAGAGATGG TGCTGTCCGA TGACAACTTT 
GCCTCCATTG TGGCTGCAGT AGAAGAGGGC AGGGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATCTCCTC CAATGTTGGC GAGGTTGTCT GCATCTTCCT CACAGCAATT 
CTGGGCCTGC CCGAAGCTCT GATCCCTGTG CAGCTGCTCT GGGTGAACCT GGTAACAGAT 
GGCTTACCGG CCACAGCCCT TGGCTTCAAC CCACCAGATC TGGATATCAT GGAGAAGCCG 
CCTCGGAATC CCCGTGAGGC CCTCATCAGT GGCTGGCTTT TTTTCCGCTA TTTGGCTATT 
GGAGTGTATG TAGGCCTGGC TACAGTGGCT GCCGCCACCT GGTGGTTCCT GTATGACACC 
GAGGGACCTC AAGTCACCTT CTATCAGCTG AGGAACTTCC TGAAGTGCTC TGAAGACAAT 
CCACTGTTTG CTGGCATTGA CTGCAAGGTT TTTGAGTCAC GCTTCCCCAC AACCATGGCC 
TTATCTGTGC TTGTGACCAT TGAAATGTGC AACGCCCTCA ACAGCGTCTC TGAGAACCAG 
TCACTGCTGC GCATGCCACC CTGGCTGAAC CCTTGGCTGC TGGGGGCTGT GGTCATGTCC 
ATGGCTCTGC ATTTTCTTAT CCTCCTGGTG CCACCTCTGC CTCTCATTTT TCAGGTGACC 
CCACTGAGTG GTCGGCAGTG GGGGGTGGTG CTTCAGATGT CTCTGCCTGT CATCCTGCTC 
GATGAGGCCC TCAAGTATCT GTCCAGGAAT CACATGGATG GTGTTCTCGG GACATTTATG 
CAGGCTCGGA GTAGGCAGCT GCCGACTACT TCCAGGACCC CATACCACAC CGGAAAAAAA 
GGACCTGAAG TAAATCCTGG GAGCAGAGGG GAGTCTCCAG TGTGGCCCTC AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2a3 ( NM_001163337.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156809.1 Mus musculus ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 3, mRNA.

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CloneID	OMu08911
Clone ID Related Accession (Same CDS sequence)	NM_001163337.1
Accession Version	NM_001163337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 3 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK155904.1, AK089487.1, AL670399.4, U49393.1, AK152999.1 and BY549777.1. Transcript Variant: This variant (3) includes an additional exon in the 3' coding region resulting in a frameshift, compared to variant 1. Variant 3 encodes isoform c, which is longer and has a distinct C-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGAGG CGCACCTGCT CTCCGCTGCC GACGTGCTGC GTCGCTTCTC GGTGACAGCC 
GAGGGCGGCT TGAGCCTGGA GCAGGTGACC GACGCGCGGG AGCGTTACGG CCCCAACGAG 
CTCCCGACCG AGGAAGGGAA ATCCCTGTGG GAGCTGGTGG TGGAGCAGTT TGAGGACCTC 
TTGGTGCGCA TCCTGCTGCT GGCAGCTCTG GTTTCCTTTG TCCTGGCCTG GTTCGAGGAG 
GGTGAGGAGA CCACAACGGC CTTCGTGGAG CCCCTGGTCA TCATGCTAAT TCTCGTGGCC 
AATGCGATCG TGGGAGTGTG GCAGGAACGC AACGCAGAGA GTGCAATTGA GGCCTTGAAG 
GAGTATGAGC CTGAGATGGG TAAGGTGATC CGGTCTGACC GCAAGGGTGT ACAGAGGATC 
CGAGCCAGGG ACATCGTCCC TGGGGACATT GTGGAAGTGG CAGTGGGAGA CAAGGTACCC 
GCTGACCTCC GTCTCATCGA GATCAAGTCC ACCACACTTC GAGTGGACCA GTCCATCCTG 
ACAGGGGAAT CCGTGTCTGT GACGAAGCAC ACAGATGCCA TTCCAGACCC CCGAGCCGTG 
AACCAGGACA AGAAGAACAT GCTATTTTCT GGCACCAATA TTGCATCAGG CAAAGCCCTG 
GGTGTGGCTG TGGCTACAGG CCTGCAGACA GAGCTGGGCA AGATTCGGAG CCAGATGGCG 
GCTGTGGAGC CTGAGCGGAC GCCATTGCAG CGCAAGCTGG ATGAATTTGG GAGGCAGCTG 
TCCCATGCCA TCTCTGTGAT CTGTGTGGCT GTGTGGGTCA TCAACATCGG CCACTTTGCT 
GACCCGGCCC ACGGTGGATC CTGGCTCCGT GGTGCAGTCT ACTACTTCAA GATCGCTGTG 
GCCCTGGCTG TGGCTGCTAT CCCCGAGGGT CTCCCAGCAG TCATCACTAC ATGCCTGGCC 
CTGGGCACAA GACGTATGGC ACGTAAGAAT GCCATTGTGC GGAGCCTGCC TTCTGTGGAG 
ACGCTGGGCT GCACCTCGGT CATCTGCTCT GACAAGACGG GGACGCTCAC CACCAATCAG 
ATGTCTGTCT GCAGGATGTT CGTGGTGGCT GAAGCAGAGG CAGGCACCTG TCGTTTGCAT 
GAATTCACCA TTTCGGGTAC CACGTACACC CCAGAGGGCG AAGTGAGGCA AGGGGAGCAG 
CCTGTGCGCT GTGGGCAGTT TGATGGGCTA GTGGAGCTCG CCACCATCTG TGCCCTCTGC 
AATGATTCTG CGCTGGACTA CAATGAGGCC AAGGGTGTGT ATGAGAAGGT AGGAGAGGCC 
ACAGAGACCG CCCTGACTTG CCTGGTAGAG AAGATGAATG TGTTTGACAC AGACCTGAAA 
GGACTGTCGC GGGTGGAGCG TGCTGGCGCC TGCAACTCAG TCATCAAGCA ACTCATGCGG 
AAAGAGTTCA CCCTGGAGTT CTCCCGGGAC CGGAAGTCTA TGTCCGTGTA CTGCACACCT 
ACTCGTGCTG ACCCCAAGGT CCAGGGCAGC AAGATGTTTG TGAAGGGAGC TCCTGAGAGT 
GTAATTGAGC GCTGTAGCTC AGTCCGAGTG GGCAGCCGAA CAGCACCCCT GAGCACCACT 
TCCAGAGAGC ATATCCTGGC CAAGATCCGG GACTGGGGCT CAGGCTCTGA CACACTGCGC 
TGCTTAGCCT TGGCCACCAG GGACACACCC CCAAGGAAGG AGGACATGCA TCTAGATGAC 
TGCAGCCGGT TTGTACAGTA CGAGACAGAC CTGACCTTCG TGGGCTGCGT GGGCATGTTA 
GACCCACCGA GACCAGAGGT GGCTGCTTGT ATCACACGCT GTTCCCGGGC TGGCATTCGA 
GTGGTCATGA TCACAGGGGA CAACAAAGGG ACAGCTGTGG CCATCTGCCG CCGACTTGGC 
ATCTTTGGGG ACACGGAGGA TGTGTTGGGC AAGGCCTACA CGGGCCGTGA GTTTGATGAC 
CTCAGCCCAG AGCAGCAGCG CCAGGCTTGT CGCACTGCCC GGTGCTTTGC CCGAGTGGAA 
CCCGCACATA AGTCTCGAAT CGTGGAGAAC CTACAGTCCT TTAATGAGAT CACTGCCATG 
ACTGGCGATG GGGTGAATGA TGCACCAGCC CTGAAGAAGG CAGAGATCGG CATCGCCATG 
GGCTCAGGCA CTGCTGTGGC CAAGTCAGCA GCAGAGATGG TGCTGTCCGA TGACAACTTT 
GCCTCCATTG TGGCTGCAGT AGAAGAGGGC AGGGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATCTCCTC CAATGTTGGC GAGGTTGTCT GCATCTTCCT CACAGCAATT 
CTGGGCCTGC CCGAAGCTCT GATCCCTGTG CAGCTGCTCT GGGTGAACCT GGTAACAGAT 
GGCTTACCGG CCACAGCCCT TGGCTTCAAC CCACCAGATC TGGATATCAT GGAGAAGCCG 
CCTCGGAATC CCCGTGAGGC CCTCATCAGT GGCTGGCTTT TTTTCCGCTA TTTGGCTATT 
GGAGTGTATG TAGGCCTGGC TACAGTGGCT GCCGCCACCT GGTGGTTCCT GTATGACACC 
GAGGGACCTC AAGTCACCTT CTATCAGCTG AGGAACTTCC TGAAGTGCTC TGAAGACAAT 
CCACTGTTTG CTGGCATTGA CTGCAAGGTT TTTGAGTCAC GCTTCCCCAC AACCATGGCC 
TTATCTGTGC TTGTGACCAT TGAAATGTGC AACGCCCTCA ACAGCGTCTC TGAGAACCAG 
TCACTGCTGC GCATGCCACC CTGGCTGAAC CCTTGGCTGC TGGGGGCTGT GGTCATGTCC 
ATGGCTCTGC ATTTTCTTAT CCTCCTGGTG CCACCTCTGC CTCTCATTTT TCAGGTGACC 
CCACTGAGTG GTCGGCAGTG GGGGGTGGTG CTTCAGATGT CTCTGCCTGT CATCCTGCTC 
GATGAGGCCC TCAAGTATCT GTCCAGGAAT CACATGGATG GTGTTCTCGG GACATTTATG 
CAGGCTCGGA GTAGGCAGCT GCCGACTACT TCCAGGACCC CATACCACAC CGGGTTGGCT 
TCTTGGAAAA AAAGGACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156809.1
CDS	186..3266
Translation	MEEAHLLSAADVLRRFSVTAEGGLSLEQVTDARERYGPNELPTEEGKSLWELVVEQFEDLLVRILLLAALVSFVLAWFEEGEETTTAFVEPLVIMLILVANAIVGVWQERNAESAIEALKEYEPEMGKVIRSDRKGVQRIRARDIVPGDIVEVAVGDKVPADLRLIEIKSTTLRVDQSILTGESVSVTKHTDAIPDPRAVNQDKKNMLFSGTNIASGKALGVAVATGLQTELGKIRSQMAAVEPERTPLQRKLDEFGRQLSHAISVICVAVWVINIGHFADPAHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFVVAEAEAGTCRLHEFTISGTTYTPEGEVRQGEQPVRCGQFDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLKGLSRVERAGACNSVIKQLMRKEFTLEFSRDRKSMSVYCTPTRADPKVQGSKMFVKGAPESVIERCSSVRVGSRTAPLSTTSREHILAKIRDWGSGSDTLRCLALATRDTPPRKEDMHLDDCSRFVQYETDLTFVGCVGMLDPPRPEVAACITRCSRAGIRVVMITGDNKGTAVAICRRLGIFGDTEDVLGKAYTGREFDDLSPEQQRQACRTARCFARVEPAHKSRIVENLQSFNEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEKPPRNPREALISGWLFFRYLAIGVYVGLATVAAATWWFLYDTEGPQVTFYQLRNFLKCSEDNPLFAGIDCKVFESRFPTTMALSVLVTIEMCNALNSVSENQSLLRMPPWLNPWLLGAVVMSMALHFLILLVPPLPLIFQVTPLSGRQWGVVLQMSLPVILLDEALKYLSRNHMDGVLGTFMQARSRQLPTTSRTPYHTGLASWKKRT

Target ORF information:

RefSeq Version	NM_001163337.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163337.1

ORF Insert Sequence:

ATGGAGGAGG CGCACCTGCT CTCCGCTGCC GACGTGCTGC GTCGCTTCTC GGTGACAGCC 
GAGGGCGGCT TGAGCCTGGA GCAGGTGACC GACGCGCGGG AGCGTTACGG CCCCAACGAG 
CTCCCGACCG AGGAAGGGAA ATCCCTGTGG GAGCTGGTGG TGGAGCAGTT TGAGGACCTC 
TTGGTGCGCA TCCTGCTGCT GGCAGCTCTG GTTTCCTTTG TCCTGGCCTG GTTCGAGGAG 
GGTGAGGAGA CCACAACGGC CTTCGTGGAG CCCCTGGTCA TCATGCTAAT TCTCGTGGCC 
AATGCGATCG TGGGAGTGTG GCAGGAACGC AACGCAGAGA GTGCAATTGA GGCCTTGAAG 
GAGTATGAGC CTGAGATGGG TAAGGTGATC CGGTCTGACC GCAAGGGTGT ACAGAGGATC 
CGAGCCAGGG ACATCGTCCC TGGGGACATT GTGGAAGTGG CAGTGGGAGA CAAGGTACCC 
GCTGACCTCC GTCTCATCGA GATCAAGTCC ACCACACTTC GAGTGGACCA GTCCATCCTG 
ACAGGGGAAT CCGTGTCTGT GACGAAGCAC ACAGATGCCA TTCCAGACCC CCGAGCCGTG 
AACCAGGACA AGAAGAACAT GCTATTTTCT GGCACCAATA TTGCATCAGG CAAAGCCCTG 
GGTGTGGCTG TGGCTACAGG CCTGCAGACA GAGCTGGGCA AGATTCGGAG CCAGATGGCG 
GCTGTGGAGC CTGAGCGGAC GCCATTGCAG CGCAAGCTGG ATGAATTTGG GAGGCAGCTG 
TCCCATGCCA TCTCTGTGAT CTGTGTGGCT GTGTGGGTCA TCAACATCGG CCACTTTGCT 
GACCCGGCCC ACGGTGGATC CTGGCTCCGT GGTGCAGTCT ACTACTTCAA GATCGCTGTG 
GCCCTGGCTG TGGCTGCTAT CCCCGAGGGT CTCCCAGCAG TCATCACTAC ATGCCTGGCC 
CTGGGCACAA GACGTATGGC ACGTAAGAAT GCCATTGTGC GGAGCCTGCC TTCTGTGGAG 
ACGCTGGGCT GCACCTCGGT CATCTGCTCT GACAAGACGG GGACGCTCAC CACCAATCAG 
ATGTCTGTCT GCAGGATGTT CGTGGTGGCT GAAGCAGAGG CAGGCACCTG TCGTTTGCAT 
GAATTCACCA TTTCGGGTAC CACGTACACC CCAGAGGGCG AAGTGAGGCA AGGGGAGCAG 
CCTGTGCGCT GTGGGCAGTT TGATGGGCTA GTGGAGCTCG CCACCATCTG TGCCCTCTGC 
AATGATTCTG CGCTGGACTA CAATGAGGCC AAGGGTGTGT ATGAGAAGGT AGGAGAGGCC 
ACAGAGACCG CCCTGACTTG CCTGGTAGAG AAGATGAATG TGTTTGACAC AGACCTGAAA 
GGACTGTCGC GGGTGGAGCG TGCTGGCGCC TGCAACTCAG TCATCAAGCA ACTCATGCGG 
AAAGAGTTCA CCCTGGAGTT CTCCCGGGAC CGGAAGTCTA TGTCCGTGTA CTGCACACCT 
ACTCGTGCTG ACCCCAAGGT CCAGGGCAGC AAGATGTTTG TGAAGGGAGC TCCTGAGAGT 
GTAATTGAGC GCTGTAGCTC AGTCCGAGTG GGCAGCCGAA CAGCACCCCT GAGCACCACT 
TCCAGAGAGC ATATCCTGGC CAAGATCCGG GACTGGGGCT CAGGCTCTGA CACACTGCGC 
TGCTTAGCCT TGGCCACCAG GGACACACCC CCAAGGAAGG AGGACATGCA TCTAGATGAC 
TGCAGCCGGT TTGTACAGTA CGAGACAGAC CTGACCTTCG TGGGCTGCGT GGGCATGTTA 
GACCCACCGA GACCAGAGGT GGCTGCTTGT ATCACACGCT GTTCCCGGGC TGGCATTCGA 
GTGGTCATGA TCACAGGGGA CAACAAAGGG ACAGCTGTGG CCATCTGCCG CCGACTTGGC 
ATCTTTGGGG ACACGGAGGA TGTGTTGGGC AAGGCCTACA CGGGCCGTGA GTTTGATGAC 
CTCAGCCCAG AGCAGCAGCG CCAGGCTTGT CGCACTGCCC GGTGCTTTGC CCGAGTGGAA 
CCCGCACATA AGTCTCGAAT CGTGGAGAAC CTACAGTCCT TTAATGAGAT CACTGCCATG 
ACTGGCGATG GGGTGAATGA TGCACCAGCC CTGAAGAAGG CAGAGATCGG CATCGCCATG 
GGCTCAGGCA CTGCTGTGGC CAAGTCAGCA GCAGAGATGG TGCTGTCCGA TGACAACTTT 
GCCTCCATTG TGGCTGCAGT AGAAGAGGGC AGGGCCATCT ACAACAACAT GAAGCAGTTC 
ATCCGCTACC TCATCTCCTC CAATGTTGGC GAGGTTGTCT GCATCTTCCT CACAGCAATT 
CTGGGCCTGC CCGAAGCTCT GATCCCTGTG CAGCTGCTCT GGGTGAACCT GGTAACAGAT 
GGCTTACCGG CCACAGCCCT TGGCTTCAAC CCACCAGATC TGGATATCAT GGAGAAGCCG 
CCTCGGAATC CCCGTGAGGC CCTCATCAGT GGCTGGCTTT TTTTCCGCTA TTTGGCTATT 
GGAGTGTATG TAGGCCTGGC TACAGTGGCT GCCGCCACCT GGTGGTTCCT GTATGACACC 
GAGGGACCTC AAGTCACCTT CTATCAGCTG AGGAACTTCC TGAAGTGCTC TGAAGACAAT 
CCACTGTTTG CTGGCATTGA CTGCAAGGTT TTTGAGTCAC GCTTCCCCAC AACCATGGCC 
TTATCTGTGC TTGTGACCAT TGAAATGTGC AACGCCCTCA ACAGCGTCTC TGAGAACCAG 
TCACTGCTGC GCATGCCACC CTGGCTGAAC CCTTGGCTGC TGGGGGCTGT GGTCATGTCC 
ATGGCTCTGC ATTTTCTTAT CCTCCTGGTG CCACCTCTGC CTCTCATTTT TCAGGTGACC 
CCACTGAGTG GTCGGCAGTG GGGGGTGGTG CTTCAGATGT CTCTGCCTGT CATCCTGCTC 
GATGAGGCCC TCAAGTATCT GTCCAGGAAT CACATGGATG GTGTTCTCGG GACATTTATG 
CAGGCTCGGA GTAGGCAGCT GCCGACTACT TCCAGGACCC CATACCACAC CGGGTTGGCT 
TCTTGGAAAA AAAGGACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread control of calcium signaling by a family of SERCA-inhibiting micropeptides.
Science signaling9(457)ra119(2016 Dec)
Anderson DM,Makarewich CA,Anderson KM,Shelton JM,Bezprozvannaya S,Bassel-Duby R,Olson EN

Full activation of mouse platelets requires ADP secretion regulated by SERCA3 ATPase-dependent calcium stores.
Blood128(8)1129-38(2016 08)
Ela?b Z,Adam F,Berrou E,Bordet JC,Pr?vost N,Bobe R,Bryckaert M,Rosa JP

A micropeptide encoded by a putative long noncoding RNA regulates muscle performance.
Cell160(4)595-606(2015 Feb)
Anderson DM,Anderson KM,Chang CL,Makarewich CA,Nelson BR,McAnally JR,Kasaragod P,Shelton JM,Liou J,Bassel-Duby R,Olson EN

Atrial natriuretic peptide locally counteracts the deleterious effects of cardiomyocyte mineralocorticoid receptor activation.
Circulation. Heart failure7(5)814-21(2014 Sep)
Nakagawa H,Oberwinkler H,Nikolaev VO,Ga?ner B,Umbenhauer S,Wagner H,Saito Y,Baba HA,Frantz S,Kuhn M

Placental PPAR? regulates spatiotemporally diverse genes and a unique metabolic network.
Developmental biology372(1)143-55(2012 Dec)
Shalom-Barak T,Zhang X,Chu T,Timothy Schaiff W,Reddy JK,Xu J,Sadovsky Y,Barak Y

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Atp2a3 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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