Gars cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gars
Entrez Gene ID	353172
Full Name	glycyl-tRNA synthetase
Synonyms	GENA202,Gena201,Nmf249,Sgrp23
General protein information	Preferred Names glycyl-tRNA synthetase Names glycine--tRNA ligase AP-4-A synthetase diadenosine tetraphosphate synthetase glyRS storage granule protein 23
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 B3\|6 27.29 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_180678.3	NP_851009.2	glycine--tRNA ligase

Pathways from BioSystems

Homologies

Homo sapiens (human)	GARS	NP_002038.2
Drosophila melanogaster (fruit fly)	Aats-gly	NP_001261867.1
Saccharomyces cerevisiae (baker's yeast)	GRS1	NP_009679.2
Magnaporthe oryzae (rice blast fungus)	MGG_06321	XP_003717264.1
Neurospora crassa	NCU00405	XP_957291.1
Xenopus tropicalis (tropical clawed frog)	gars	NP_001007492.1
Pan troglodytes (chimpanzee)	GARS	XP_519025.3
Bos taurus (cattle)	GARS	NP_001091035.1
Rattus norvegicus (Norway rat)	Gars	NP_001258068.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008604	XP_557743.2
Caenorhabditis elegans (roundworm)	gars-1	NP_498093.1
Gallus gallus (chicken)	GARS	NP_001026681.1
Oryza sativa (rice)	Os08g0538000	NP_001062369.1
Kluyveromyces lactis	KLLA0B08932g	XP_451926.1
Eremothecium gossypii	AGOS_ADL373W	NP_983723.1
Schizosaccharomyces pombe (fission yeast)	grs1	NP_593935.1
Canis lupus familiaris (dog)	GARS	XP_003639538.1
Mus musculus (house mouse)	Gars	NP_851009.2
Danio rerio (zebrafish)	gars	XP_692410.4
Arabidopsis thaliana (thale cress)	AT1G29880	NP_564337.1

Gene Ontology
Bibliography

Related articles in PubMed

Neuropilin 1 sequestration by neuropathogenic mutant glycyl-tRNA synthetase is permissive to vascular homeostasis.
Sleigh JN, G?mez-Mart?n A, Wei N, Bai G, Yang XL, Schiavo G
Scientific reports7(1)9216(2017 Aug)

Trk receptor signaling and sensory neuron fate are perturbed in human neuropathy caused by
Sleigh JN, Dawes JM, West SJ, Wei N, Spaulding EL, G?mez-Mart?n A, Zhang Q, Burgess RW, Cader MZ, Talbot K, Yang XL, Bennett DL, Schiavo G
Proceedings of the National Academy of Sciences of the United States of America114(16)E3324-E3333(2017 Apr)

Severity of Demyelinating and Axonal Neuropathy Mouse Models Is Modified by Genes Affecting Structure and Function of Peripheral Nodes.
Morelli KH, Seburn KL, Schroeder DG, Spaulding EL, Dionne LA, Cox GA, Burgess RW
Cell reports18(13)3178-3191(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings link the selective pathology of Charcot-Marie-Tooth disease type 2D to the neomorphic binding activity of GlyRS(CMT2D) that antagonizes the VEGF-Nrp1 interaction, meaning the VEGF-Nrp1 signalling axis is an actionable target for treating CMT2D
Title: CMT2D neuropathy is linked to the neomorphic binding activity of glycyl-tRNA synthetase.

These results suggest that this animal model of CMT using an adenovirus may provide information regarding CMT as well as a useful strategy for the treatment of neuropathic pain.
Title: Neuropathic pain model of peripheral neuropathies mediated by mutations of glycyl-tRNA synthetase.

Gars(C201R) mutation significantly delayed disease onset in the SOD1(G93A);Gars(C201R/+) double heterozygous mutant mice and increased lifespan by 29% on the genetic background investigated
Title: Mutant glycyl-tRNA synthetase (Gars) ameliorates SOD1(G93A) motor neuron degeneration phenotype but has little affect on Loa dynein heavy chain mutant mice.

a glycyl-tRNA synthetase (GARS) mutation causes peripheral sensory and motor phenotypes creating a model of Charcot-Marie-Tooth type 2D peripheral neuropathy
Title: An ENU-induced mutation in mouse glycyl-tRNA synthetase (GARS) causes peripheral sensory and motor phenotypes creating a model of Charcot-Marie-Tooth type 2D peripheral neuropathy.

Results indicate that Charcot-Marie-Tooth peripheral neuropathies, type 2D (CMT2D) phenotype is caused by novel pathogenic roles for the mutant GARS that specifically affect peripheral neurons.
Title: An active dominant mutation of glycyl-tRNA synthetase causes neuropathy in a Charcot-Marie-Tooth 2D mouse model.

The following Gars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07808	NM_180678.3 Latest version!	Mus musculus glycyl-tRNA synthetase (Gars), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$433.30 $619.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gars ( NM_180678.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_851009.2 Mus musculus glycyl-tRNA synthetase (Gars), mRNA.

Price & Availability↑

CloneID	OMu07808
Clone ID Related Accession (Same CDS sequence)	NM_180678.3
Accession Version	NM_180678.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	glycine--tRNA ligase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY309346.1, AK012758.2 and AV236862.1. On Apr 21, 2006 this sequence version replaced NM_180678.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC021747.1, AK146238.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTGTC TGCTCCCCTC GCTGCTCAGA GCCACCCGCG CCGCGCTGCC GCTCCTGTCG 
CCGCCCCGAG TGGTCGCAGC GTCCGCGTCT CAGCGGCTCC TCAGCGCTCC CGCGCAGCCC 
GCCGCCTCCC GGAGCAGCAT GGACAGCGCG GAGGAGCTGC TGGCCCCACT GCGGCTAGCC 
GTGCGCCAGC AGGGAGACTT TGTACGGAAG TTAAAAGAAG ATAAAGCCCC ACAAGTGGAT 
GTGGACAGAG CAGTAGCTGA GCTCAAGGCC CGGAAGAGGG TTCTGGAAGC AAAGGAGCTG 
GCATTGCAGC CCAAAGATGA CATCGTAGAT AGAGCAAAAA TGGAAGATAC GTTGAAGAGG 
AGGTTTTTCT ACGATCAGGC TTTTGCTATT TATGGAGGTG TCAGTGGATT GTATGACTTC 
GGGCCGGTAG GATGTGCTTT GAAGAACAAT ATCATCCAGG CCTGGAGGCA GCACTTTATC 
CAAGAGGAGC AGATCCTGGA GATTGACTGC ACCATGCTCA CCCCTGAGCC AGTTTTAAAG 
ACCTCTGGCC ACGTAGACAA ATTTGCTGAC TTCATGGTGA AGGACGTGAA GAACGGAGAG 
TGCTTCCGAG CAGACCACCT GTTGAAAGCT CATTTACAGA AACTGATGTC AGATAAGAAG 
TGCTCTGCTG AGAAGAAGTC AGAGATGGAA AGTGTCTTGG CCCAGCTTGA TAACTATGGA 
CAACAAGAAC TTGCGGATCT TTTTGTGAAC TATAATGTAA AATCTCCCAC CACTGGCAAT 
GACCTGTCCC CTCCGGTACC TTTTAACTTA ATGTTCCAGA CCTTCATTGG GCCTGGAGGA 
AATATGCCTG GATATCTGAG ACCAGAAACT GCACAGGGAA TTTTCCTGAA TTTCAAACGA 
CTTTTGGAAT TCAACCAAGG GAAATTGCCT TTTGCTGCTG CCCAGATTGG AAACTCCTTC 
AGAAATGAGA TCTCACCTCG GTCTGGACTG ATCCGAGTCA GGGAGTTTAC AATGGCAGAG 
ATTGAGCACT TTGTAGATCC CACTGAGAAA GACCATCCCA AGTTCCAAAG TGTGGCCGAC 
CTCTGCCTTT ATTTGTACTC AGCAAAAGCC CAGGTCACTG GACAGTCTGC TCGGAAGATG 
CGTCTGGGAG ATGCTGTTGA ACAGGGTGTG ATTAACAACT CAGTATTAGG CTATTTTATT 
GGCCGCATCT ACCTCTACCT CACGAAGGTT GGAATATCTC CTGATAAACT CCGCTTCCGG 
CAGCATATGG AGAATGAGAT GGCCCATTAT GCCTGCGACT GCTGGGATGC CGAGTCCAAA 
ACGTCCTATG GCTGGATTGA GATTGTTGGA TGTGCTGACC GTTCCTGCTA CGACCTCTCC 
TGTCATGCTC GAGCCACCAA AGTTCCACTA GTAGCTGAGA AACCTCTGAA AGAACCCAAA 
ACAGTTAACG TTGTACAGTT TGAGCCCAAC AAGGGCGCCG TGGGCAAGGC GTACAAGAAG 
GATGCAAAGC TAGTGCTGGA GTATCTCAGC GCCTGTGATG AGTGCTACAT TTCAGAGATG 
GAGCTGCTGC TGAGTGAGAA AGGGGAATTC ACTATTGAAA CTGAAGGAAA AACATTTCAG 
TTAACGAAAG ACATGGTCAG TGTGAAGAGA TTCCAGAAAA CACTGCATGT GGAAGAAGTT 
GTTCCGAGTG TAATTGAGCC CTCCTTTGGC TTGGGCAGGA TCATGTATAC CATATTGGAA 
CATACATTCC ATGTCCGAGA GGGAGATGAA CAGAGAACGT TCTTCAGTTT CCCTGCTGTG 
GTTGCTCCAT TCAAATGTTC TGTCCTTCCA CTGAGCCAGA ACCAAGAGTT TATGCCATTT 
GTCAAGGAAT TATCCGAAGC TCTGACCAGG AACGGCGTGT CTCATAAAGT CGATGACTCC 
TCTGGGTCTA TTGGAAGGCG CTATGCAAGA ACTGATGAGA TTGGCGTGGC TTTCGGCATC 
ACTATTGACT TTGATACAGT GAACAAGACG CCCCACACTG CAACTCTGAG GGACCGAGAC 
TCCATGAGAC AGATAAGGGC AGAGGTCTCT GAGCTGCCCA ATGTGGTCCG CGATCTGGCC 
AACGGCAACA TTACCTGGGC TGATGTGGAG GCCAGGTACC CACTCTTTGA AGGGCAAGAG 
ACTGGCAAGA AGGAGACAGT AGAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851009.2
CDS	42..2231
Translation	MPCLLPSLLRATRAALPLLSPPRVVAASASQRLLSAPAQPAASRSSMDSAEELLAPLRLAVRQQGDFVRKLKEDKAPQVDVDRAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQAWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSAEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPTTGNDLSPPVPFNLMFQTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPTEKDHPKFQSVADLCLYLYSAKAQVTGQSARKMRLGDAVEQGVINNSVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPNKGAVGKAYKKDAKLVLEYLSACDECYISEMELLLSEKGEFTIETEGKTFQLTKDMVSVKRFQKTLHVEEVVPSVIEPSFGLGRIMYTILEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRNGVSHKVDDSSGSIGRRYARTDEIGVAFGITIDFDTVNKTPHTATLRDRDSMRQIRAEVSELPNVVRDLANGNITWADVEARYPLFEGQETGKKETVEE

Target ORF information:

RefSeq Version	NM_180678.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glycyl-tRNA synthetase (Gars), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180678.3

ORF Insert Sequence:

ATGCCCTGTC TGCTCCCCTC GCTGCTCAGA GCCACCCGCG CCGCGCTGCC GCTCCTGTCG 
CCGCCCCGAG TGGTCGCAGC GTCCGCGTCT CAGCGGCTCC TCAGCGCTCC CGCGCAGCCC 
GCCGCCTCCC GGAGCAGCAT GGACAGCGCG GAGGAGCTGC TGGCCCCACT GCGGCTAGCC 
GTGCGCCAGC AGGGAGACTT TGTACGGAAG TTAAAAGAAG ATAAAGCCCC ACAAGTGGAT 
GTGGACAGAG CAGTAGCTGA GCTCAAGGCC CGGAAGAGGG TTCTGGAAGC AAAGGAGCTG 
GCATTGCAGC CCAAAGATGA CATCGTAGAT AGAGCAAAAA TGGAAGATAC GTTGAAGAGG 
AGGTTTTTCT ACGATCAGGC TTTTGCTATT TATGGAGGTG TCAGTGGATT GTATGACTTC 
GGGCCGGTAG GATGTGCTTT GAAGAACAAT ATCATCCAGG CCTGGAGGCA GCACTTTATC 
CAAGAGGAGC AGATCCTGGA GATTGACTGC ACCATGCTCA CCCCTGAGCC AGTTTTAAAG 
ACCTCTGGCC ACGTAGACAA ATTTGCTGAC TTCATGGTGA AGGACGTGAA GAACGGAGAG 
TGCTTCCGAG CAGACCACCT GTTGAAAGCT CATTTACAGA AACTGATGTC AGATAAGAAG 
TGCTCTGCTG AGAAGAAGTC AGAGATGGAA AGTGTCTTGG CCCAGCTTGA TAACTATGGA 
CAACAAGAAC TTGCGGATCT TTTTGTGAAC TATAATGTAA AATCTCCCAC CACTGGCAAT 
GACCTGTCCC CTCCGGTACC TTTTAACTTA ATGTTCCAGA CCTTCATTGG GCCTGGAGGA 
AATATGCCTG GATATCTGAG ACCAGAAACT GCACAGGGAA TTTTCCTGAA TTTCAAACGA 
CTTTTGGAAT TCAACCAAGG GAAATTGCCT TTTGCTGCTG CCCAGATTGG AAACTCCTTC 
AGAAATGAGA TCTCACCTCG GTCTGGACTG ATCCGAGTCA GGGAGTTTAC AATGGCAGAG 
ATTGAGCACT TTGTAGATCC CACTGAGAAA GACCATCCCA AGTTCCAAAG TGTGGCCGAC 
CTCTGCCTTT ATTTGTACTC AGCAAAAGCC CAGGTCACTG GACAGTCTGC TCGGAAGATG 
CGTCTGGGAG ATGCTGTTGA ACAGGGTGTG ATTAACAACT CAGTATTAGG CTATTTTATT 
GGCCGCATCT ACCTCTACCT CACGAAGGTT GGAATATCTC CTGATAAACT CCGCTTCCGG 
CAGCATATGG AGAATGAGAT GGCCCATTAT GCCTGCGACT GCTGGGATGC CGAGTCCAAA 
ACGTCCTATG GCTGGATTGA GATTGTTGGA TGTGCTGACC GTTCCTGCTA CGACCTCTCC 
TGTCATGCTC GAGCCACCAA AGTTCCACTA GTAGCTGAGA AACCTCTGAA AGAACCCAAA 
ACAGTTAACG TTGTACAGTT TGAGCCCAAC AAGGGCGCCG TGGGCAAGGC GTACAAGAAG 
GATGCAAAGC TAGTGCTGGA GTATCTCAGC GCCTGTGATG AGTGCTACAT TTCAGAGATG 
GAGCTGCTGC TGAGTGAGAA AGGGGAATTC ACTATTGAAA CTGAAGGAAA AACATTTCAG 
TTAACGAAAG ACATGGTCAG TGTGAAGAGA TTCCAGAAAA CACTGCATGT GGAAGAAGTT 
GTTCCGAGTG TAATTGAGCC CTCCTTTGGC TTGGGCAGGA TCATGTATAC CATATTGGAA 
CATACATTCC ATGTCCGAGA GGGAGATGAA CAGAGAACGT TCTTCAGTTT CCCTGCTGTG 
GTTGCTCCAT TCAAATGTTC TGTCCTTCCA CTGAGCCAGA ACCAAGAGTT TATGCCATTT 
GTCAAGGAAT TATCCGAAGC TCTGACCAGG AACGGCGTGT CTCATAAAGT CGATGACTCC 
TCTGGGTCTA TTGGAAGGCG CTATGCAAGA ACTGATGAGA TTGGCGTGGC TTTCGGCATC 
ACTATTGACT TTGATACAGT GAACAAGACG CCCCACACTG CAACTCTGAG GGACCGAGAC 
TCCATGAGAC AGATAAGGGC AGAGGTCTCT GAGCTGCCCA ATGTGGTCCG CGATCTGGCC 
AACGGCAACA TTACCTGGGC TGATGTGGAG GCCAGGTACC CACTCTTTGA AGGGCAAGAG 
ACTGGCAAGA AGGAGACAGT AGAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neuropilin 1 sequestration by neuropathogenic mutant glycyl-tRNA synthetase is permissive to vascular homeostasis.
Scientific reports7(1)9216(2017 Aug)
Sleigh JN,G?mez-Mart?n A,Wei N,Bai G,Yang XL,Schiavo G

Trk receptor signaling and sensory neuron fate are perturbed in human neuropathy caused by
Proceedings of the National Academy of Sciences of the United States of America114(16)E3324-E3333(2017 Apr)
Sleigh JN,Dawes JM,West SJ,Wei N,Spaulding EL,G?mez-Mart?n A,Zhang Q,Burgess RW,Cader MZ,Talbot K,Yang XL,Bennett DL,Schiavo G

Severity of Demyelinating and Axonal Neuropathy Mouse Models Is Modified by Genes Affecting Structure and Function of Peripheral Nodes.
Cell reports18(13)3178-3191(2017 Mar)
Morelli KH,Seburn KL,Schroeder DG,Spaulding EL,Dionne LA,Cox GA,Burgess RW