Pnpla7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pnpla7
Entrez Gene ID	241274
Full Name	patatin-like phospholipase domain containing 7
Synonyms	BC027342,E430013P11Rik,Nre
General protein information	Preferred Names patatin-like phospholipase domain containing 7 Names patatin-like phospholipase domain-containing protein 7 NTE-related esterase neuropathy target esterase-related esterase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006498021.3	XP_006498084.1	patatin-like phospholipase domain-containing protein 7 isoform X7
XM_006498023.3	XP_006498086.1	patatin-like phospholipase domain-containing protein 7 isoform X11
XM_006498016.4	XP_006498079.1	patatin-like phospholipase domain-containing protein 7 isoform X4
XM_006498018.4	XP_006498081.1	patatin-like phospholipase domain-containing protein 7 isoform X5
XM_006498021.4	XP_006498084.1	patatin-like phospholipase domain-containing protein 7 isoform X8
XM_017318030.2	XP_017173519.1	patatin-like phospholipase domain-containing protein 7 isoform X10
XM_006498022.3	XP_006498085.1	patatin-like phospholipase domain-containing protein 7 isoform X11
XM_030250977.1	XP_030106837.1	patatin-like phospholipase domain-containing protein 7 isoform X14
XM_006498020.4	XP_006498083.1	patatin-like phospholipase domain-containing protein 7 isoform X6
XM_006498014.3	XP_006498077.1	patatin-like phospholipase domain-containing protein 7 isoform X1
XM_011239092.2	XP_011237394.1	patatin-like phospholipase domain-containing protein 7 isoform X2
XM_006498016.3	XP_006498079.1	patatin-like phospholipase domain-containing protein 7 isoform X4
XM_006498019.3	XP_006498082.1	patatin-like phospholipase domain-containing protein 7 isoform X5
XM_017318030.1	XP_017173519.1	patatin-like phospholipase domain-containing protein 7 isoform X9
XM_006498022.2	XP_006498085.1	patatin-like phospholipase domain-containing protein 7 isoform X10
XM_006498014.4	XP_006498077.1	patatin-like phospholipase domain-containing protein 7 isoform X1
XM_011239092.3	XP_011237394.1	patatin-like phospholipase domain-containing protein 7 isoform X2
XM_006498017.4	XP_006498080.1	patatin-like phospholipase domain-containing protein 7 isoform X5
XM_006498019.4	XP_006498082.1	patatin-like phospholipase domain-containing protein 7 isoform X5
XM_006498018.3	XP_006498081.1	patatin-like phospholipase domain-containing protein 7 isoform X5
XM_006498020.3	XP_006498083.1	patatin-like phospholipase domain-containing protein 7 isoform X6
XM_017318029.2	XP_017173518.1	patatin-like phospholipase domain-containing protein 7 isoform X9
XM_006498024.1	XP_006498087.1	patatin-like phospholipase domain-containing protein 7 isoform X13
XM_006498015.3	XP_006498078.1	patatin-like phospholipase domain-containing protein 7 isoform X3
XM_006498017.3	XP_006498080.1	patatin-like phospholipase domain-containing protein 7 isoform X5
XM_017318029.1	XP_017173518.1	patatin-like phospholipase domain-containing protein 7 isoform X8
XM_006498015.4	XP_006498078.1	patatin-like phospholipase domain-containing protein 7 isoform X3
XM_030250966.1	XP_030106826.1	patatin-like phospholipase domain-containing protein 7 isoform X7
XM_006498023.4	XP_006498086.1	patatin-like phospholipase domain-containing protein 7 isoform X12
NM_146251.4	NP_666363.3	patatin-like phospholipase domain-containing protein 7

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PNPLA7	XP_003586736.2
Mus musculus (house mouse)	Pnpla7	NP_666363.3
Rattus norvegicus (Norway rat)	Pnpla7	NP_653339.2
Xenopus tropicalis (tropical clawed frog)	pnpla6	NP_001090744.2
Homo sapiens (human)	PNPLA7	NP_001092007.1
Canis lupus familiaris (dog)	PNPLA7	XP_537782.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000467	XP_310627.5
Caenorhabditis elegans (roundworm)	ZK370.4	NP_498926.2
Drosophila melanogaster (fruit fly)	sws	NP_511075.3
	LOC721508	XP_001117679.2
Gallus gallus (chicken)	PNPLA7	NP_001124214.1
Danio rerio (zebrafish)	pnpla7b	XP_692276.5
Caenorhabditis elegans (roundworm)	M110.7	NP_495734.3

Gene Ontology
Bibliography

Related articles in PubMed

Degradation of mouse NTE-related esterase by macroautophagy and the proteasome.
Chang PA, Chen YY, Long DX, Qin WZ, Mou XL
Molecular biology reports39(6)7125-31(2012 Jun)

Identification of two novel splicing variants of murine NTE-related esterase.
Chang PA, Wang ZX, Long DX, Qin WZ, Wei CY, Wu YJ
Gene497(2)164-71(2012 Apr)

Identification and characterization of a splice variant of the catalytic domain of mouse NTE-related esterase.
Chang PA, Long DX, Sun Q, Wang Q, Bu YQ, Wu YJ
Gene417(1-2)43-50(2008 Jul)

Identification of an insulin-regulated lysophospholipase with homology to neuropathy target esterase.
Kienesberger PC, Lass A, Preiss-Landl K, Wolinski H, Kohlwein SD, Zimmermann R, Zechner R
The Journal of biological chemistry283(9)5908-17(2008 Feb)

Molecular cloning and expression of the C-terminal domain of mouse NTE-related esterase.
Chang PA, Long DX, Wu YJ
Molecular and cellular biochemistry306(1-2)25-32(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The transmembrane and regulatory domain played a role in the degradation of mouse NTE-related esterase by macroautophagy and the proteasome respectively.
Title: Degradation of mouse NTE-related esterase by macroautophagy and the proteasome.

There was a nutritional regulation of murine NTE-related esterase expression at the mRNA levels via alternative splicing.
Title: Identification of two novel splicing variants of murine NTE-related esterase.

A splice variant of the catalytic domain of mouse NRE (mNRECV) was identified in multiple adult tissues, including brain, kidney, liver and testis.
Title: Identification and characterization of a splice variant of the catalytic domain of mouse NTE-related esterase.

The following Pnpla7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pnpla7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu87866	XM_030250977.1 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu29537	XM_006498014.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu29538	XM_006498015.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu29539	XM_006498016.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu29540	XM_006498019.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu29540	XM_006498017.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu29540	XM_006498018.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu29541	XM_006498020.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu29542	XM_006498021.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu29543	XM_006498022.3 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu29544	XM_006498023.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu74054	XM_011239092.3 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu80246	XM_017318029.2 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu80247	XM_017318030.2 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu87865	XM_030250966.1 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu29545	XM_006498024.1 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu15337	NM_146251.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$790.30 $1129.00
View Old Accession Versions >>
OMu29542	XM_006498021.3	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29539	XM_006498016.3	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu80247	XM_017318030.1	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu29543	XM_006498022.2	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29537	XM_006498014.3	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74054	XM_011239092.2	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu29540	XM_006498019.3	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu29540	XM_006498018.3	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu29541	XM_006498020.3	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu29540	XM_006498017.3	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu80246	XM_017318029.1	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu29538	XM_006498015.3	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu29544	XM_006498023.3	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pnpla7 ( XM_030250977.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106837.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu87866
Clone ID Related Accession (Same CDS sequence)	XM_030250977.1
Accession Version	XM_030250977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTA TCTTTGTTCT TTCCAAGTCC AAGAAAAGTG TTGTCGCAGA GACCCCCTCT 
GCCATCTTCC ACTACTCAGA AAATTTCAGG GATGAGACTG GGGCCTGTGG GAAGACAGAT 
GCTATCTTCA GAGCTGCCAC AAAGGACCTC CTTACCCTGA TGAAGCTAGA TGACCCATCC 
CTGTTGGATG GCCGGGTGGC ATTTCTTCAT GTTCCTGCAG GCACCTTAGT GTCAAAACAG 
GGAGATCAGG ATGTCAATAT TTTGTTTGTG GTCTCGGGGA TGCTGCATGT GTACCAGCAA 
AAGATTGACA GCTTGGAGGA TACTTGCCTG TTCCTCACAC ATCCCGGGGA GATGGTGGGT 
CAGCTGGCGG TGCTCACTGG AGAACCCCTA ATGTTCACCA TCAGAGCCAA CCGAGACTGC 
AGCTTTTTGT CCATCTCGAA GGCCCACTTC TATGAGATCA TGAGGAAACG GCCGGATGTT 
GTCCTGGGCG TGGCACACAC AGTTGTGAAG AGGATGTCAT CCTTTGTACG GCAGATTGAT 
TTTGCTCTAG ACTGGATGGA AGTAGAGGCT GGACGTGCCA TATACAGGCA AGGAGACAAG 
TCTGACTGCA CATACATCGT TCTCAGTGGC AGGCTGCGTT CTGTCATCCG AAAAGATGAT 
GGGAAAAAAC GCCTGGCAGG GGAATATGGC CGAGGAGACC TTGTTGGTGT GGTGGAGACG 
CTGACCCACC AGGCCAGAGC AACCACAGTG CATGCTGTCC GGGACTCAGA ACTGGCTAAG 
CTGCCAGCAG GAGCTCTGAC GTCTATCAAG CGCAGGTACC CACAGGTTGT AACGCGACTG 
ATTCATCTCT TGGGCGAGAA GATCTTAGGC AGTCTCCAGC AGGGATCTGC AACAGGTCAC 
CAGCTTGGAT TTAACACAGC AAGCAGCAAA TGGGACTTGG GGAACCCGCC TGGCAATCTG 
TCCACAGTGG CAGCACTGCC TGCTTCTGAG GATGTGCCCC TAACCGCCTT TGCTCTGGAG 
CTCCAACATG CCCTCAGTGC CATCGGTCCT GTCCTGCTGC TGACCAGTGA CAACATCAAA 
CAACGCCTGG GTTCCGCTGC TCTAGACAGC ATCCATGAGT ACCGGCTCTC CAGTTGGCTG 
GGCCAGCAGG AGGACATCCA CCGGATTGTG CTGTATCAGG CAGACGGCAC ACTCACACCG 
TGGACCCAGC GCTGCATCCG GCAGGCTGAC TGCATCCTCA TCGTGGGCTT GGGTGAGCAA 
GAGCCAGCAG TAGGTGAGCT GGAGCAGATG TTGGAGAGCA CAGCTGTGCG TGCCCAGAAG 
CAGCTAATCT TGCTGCACAA AGAGGACGGT CCAGTGCCTT CCCGCACAGT GGAGTGGCTC 
AACATGCGAA GTTGGTGTTC TGGCCATTTG CACCTCTGCT GCCCACGCCG TGTCTTCTCC 
AAGAGAAGCT TGCCCAAGCT GGTCGAGATG TACACACGTG TTTTCCAACG ACCACCAGAC 
CGTCATTCTG ATTTTTCCCG CCTGGCACGG ATGCTGACAG GCAATGCCAT TGCCTTGGTG 
CTTGGAGGGG GTGGAGCTAG AGGGTGTGCC CAGGTGGGCA TCCTCAGGGC ACTAGCAGAG 
TGTGGCGTCC CCGTGGACAT AATCGGAGGC ACATCCATCG GGGCTTTCAT GGGTGCTCTG 
TTTGCTGAGG AGCGGAGCTA CAGCCAAACA CGGATCCGGG CCAAGCAGTG GGCAGAGGGT 
ATGACATCAA TGATGAAGAC TATACTGGAC CTGACCTACC CAATCACATC CATGTTCTCT 
GGCACTGGCT TCAATAGCAG CATCAGCAAC ATCTTCAAAG ACCGGCAGAT TGAGGACCTG 
TGGCTCCCCT ACTTTGCCAT CACCACCGAC ATCACGGCCT CTGCCATGCG GGTTCACACA 
GATGGCTCTC TGTGGAGGTA TGTCCGTGCA AGCATGTCCT TGTCAGGCTA TATGCCCCCA 
CTCTGCGACC CCAAGGATGG ACACCTGCTG ATGGATGGTG GTTACATCAA CAACCTCCCA 
GCGGATGTGG CAAGATCCAT GGGGGCAAAG GTTGTGATTG CCATCGATGT TGGCAGCCGG 
GACGAGACAG ACCTCACCAA CTATGGCGAT GCCTTGTCTG GATGGTGGCT GCTTTGGAAA 
CGCTGGAACC CCTTGGCTAC AAAAGTCAAG GTGTTGAACA TGGCAGAGAT TCAGACTCGA 
CTGGCCTATG TGTGCTGCGT CCGGCAGCTG GAGATGGTGA AGAACAGCGA CTACTGTGAG 
TACCTGAGGC CTCCCATCGA CAGTTACCGC ACCCTGGACT TCGGCAAATT TGATGAGATC 
TGTGAAGTTG GGTATCAACA TGGGCGGACA GTGTTTGACA TCTGGGTCCG GAGTGGTGTC 
CTAGAGAAGA TGTTGCAGGA CCAGCAGGGG ACAAGCAAGA GGAAGGACTG TGGGGTGTTC 
ACCTGCCCCA ATTCCTCCTT CACGGACCTT GCTGAGATTG TGTCACGGAT TGAGCCGGCC 
AAGGTGGCTG CAGTGGATGC CTTGCTTGGC ACAGCCACTG TCCTTGGAGA GAGGACCAAT 
GGAGCCCTCT CTTCTGCTCT AGATGAGTCT GACTATCAGA CCGAGTATGA GGAGGAACTG 
CCAGCCATCC CCAAGGAAAC GTATGCTGAC TTCCAGAGCA CTGGGATTGA GCTAGACTCT 
GACTCGGAAT ATGAGCCTTC GATGTTGCAA GGACCCCCCA GCTTGACTTC CCCAGAACAG 
TCCCAGGACT CCTTCCCTTG GCTCCCTAAT CAAGATGACC AGGGGCCAAG ACTGGAACAT 
CCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106837.1
CDS	1761..4589
Translation	MMCIFVLSKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_030250977.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250977.1

ORF Insert Sequence:

ATGATGTGTA TCTTTGTTCT TTCCAAGTCC AAGAAAAGTG TTGTCGCAGA GACCCCCTCT 
GCCATCTTCC ACTACTCAGA AAATTTCAGG GATGAGACTG GGGCCTGTGG GAAGACAGAT 
GCTATCTTCA GAGCTGCCAC AAAGGACCTC CTTACCCTGA TGAAGCTAGA TGACCCATCC 
CTGTTGGATG GCCGGGTGGC ATTTCTTCAT GTTCCTGCAG GCACCTTAGT GTCAAAACAG 
GGAGATCAGG ATGTCAATAT TTTGTTTGTG GTCTCGGGGA TGCTGCATGT GTACCAGCAA 
AAGATTGACA GCTTGGAGGA TACTTGCCTG TTCCTCACAC ATCCCGGGGA GATGGTGGGT 
CAGCTGGCGG TGCTCACTGG AGAACCCCTA ATGTTCACCA TCAGAGCCAA CCGAGACTGC 
AGCTTTTTGT CCATCTCGAA GGCCCACTTC TATGAGATCA TGAGGAAACG GCCGGATGTT 
GTCCTGGGCG TGGCACACAC AGTTGTGAAG AGGATGTCAT CCTTTGTACG GCAGATTGAT 
TTTGCTCTAG ACTGGATGGA AGTAGAGGCT GGACGTGCCA TATACAGGCA AGGAGACAAG 
TCTGACTGCA CATACATCGT TCTCAGTGGC AGGCTGCGTT CTGTCATCCG AAAAGATGAT 
GGGAAAAAAC GCCTGGCAGG GGAATATGGC CGAGGAGACC TTGTTGGTGT GGTGGAGACG 
CTGACCCACC AGGCCAGAGC AACCACAGTG CATGCTGTCC GGGACTCAGA ACTGGCTAAG 
CTGCCAGCAG GAGCTCTGAC GTCTATCAAG CGCAGGTACC CACAGGTTGT AACGCGACTG 
ATTCATCTCT TGGGCGAGAA GATCTTAGGC AGTCTCCAGC AGGGATCTGC AACAGGTCAC 
CAGCTTGGAT TTAACACAGC AAGCAGCAAA TGGGACTTGG GGAACCCGCC TGGCAATCTG 
TCCACAGTGG CAGCACTGCC TGCTTCTGAG GATGTGCCCC TAACCGCCTT TGCTCTGGAG 
CTCCAACATG CCCTCAGTGC CATCGGTCCT GTCCTGCTGC TGACCAGTGA CAACATCAAA 
CAACGCCTGG GTTCCGCTGC TCTAGACAGC ATCCATGAGT ACCGGCTCTC CAGTTGGCTG 
GGCCAGCAGG AGGACATCCA CCGGATTGTG CTGTATCAGG CAGACGGCAC ACTCACACCG 
TGGACCCAGC GCTGCATCCG GCAGGCTGAC TGCATCCTCA TCGTGGGCTT GGGTGAGCAA 
GAGCCAGCAG TAGGTGAGCT GGAGCAGATG TTGGAGAGCA CAGCTGTGCG TGCCCAGAAG 
CAGCTAATCT TGCTGCACAA AGAGGACGGT CCAGTGCCTT CCCGCACAGT GGAGTGGCTC 
AACATGCGAA GTTGGTGTTC TGGCCATTTG CACCTCTGCT GCCCACGCCG TGTCTTCTCC 
AAGAGAAGCT TGCCCAAGCT GGTCGAGATG TACACACGTG TTTTCCAACG ACCACCAGAC 
CGTCATTCTG ATTTTTCCCG CCTGGCACGG ATGCTGACAG GCAATGCCAT TGCCTTGGTG 
CTTGGAGGGG GTGGAGCTAG AGGGTGTGCC CAGGTGGGCA TCCTCAGGGC ACTAGCAGAG 
TGTGGCGTCC CCGTGGACAT AATCGGAGGC ACATCCATCG GGGCTTTCAT GGGTGCTCTG 
TTTGCTGAGG AGCGGAGCTA CAGCCAAACA CGGATCCGGG CCAAGCAGTG GGCAGAGGGT 
ATGACATCAA TGATGAAGAC TATACTGGAC CTGACCTACC CAATCACATC CATGTTCTCT 
GGCACTGGCT TCAATAGCAG CATCAGCAAC ATCTTCAAAG ACCGGCAGAT TGAGGACCTG 
TGGCTCCCCT ACTTTGCCAT CACCACCGAC ATCACGGCCT CTGCCATGCG GGTTCACACA 
GATGGCTCTC TGTGGAGGTA TGTCCGTGCA AGCATGTCCT TGTCAGGCTA TATGCCCCCA 
CTCTGCGACC CCAAGGATGG ACACCTGCTG ATGGATGGTG GTTACATCAA CAACCTCCCA 
GCGGATGTGG CAAGATCCAT GGGGGCAAAG GTTGTGATTG CCATCGATGT TGGCAGCCGG 
GACGAGACAG ACCTCACCAA CTATGGCGAT GCCTTGTCTG GATGGTGGCT GCTTTGGAAA 
CGCTGGAACC CCTTGGCTAC AAAAGTCAAG GTGTTGAACA TGGCAGAGAT TCAGACTCGA 
CTGGCCTATG TGTGCTGCGT CCGGCAGCTG GAGATGGTGA AGAACAGCGA CTACTGTGAG 
TACCTGAGGC CTCCCATCGA CAGTTACCGC ACCCTGGACT TCGGCAAATT TGATGAGATC 
TGTGAAGTTG GGTATCAACA TGGGCGGACA GTGTTTGACA TCTGGGTCCG GAGTGGTGTC 
CTAGAGAAGA TGTTGCAGGA CCAGCAGGGG ACAAGCAAGA GGAAGGACTG TGGGGTGTTC 
ACCTGCCCCA ATTCCTCCTT CACGGACCTT GCTGAGATTG TGTCACGGAT TGAGCCGGCC 
AAGGTGGCTG CAGTGGATGC CTTGCTTGGC ACAGCCACTG TCCTTGGAGA GAGGACCAAT 
GGAGCCCTCT CTTCTGCTCT AGATGAGTCT GACTATCAGA CCGAGTATGA GGAGGAACTG 
CCAGCCATCC CCAAGGAAAC GTATGCTGAC TTCCAGAGCA CTGGGATTGA GCTAGACTCT 
GACTCGGAAT ATGAGCCTTC GATGTTGCAA GGACCCCCCA GCTTGACTTC CCCAGAACAG 
TCCCAGGACT CCTTCCCTTG GCTCCCTAAT CAAGATGACC AGGGGCCAAG ACTGGAACAT 
CCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498014.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498077.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X1, mRNA.

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CloneID	OMu29537
Clone ID Related Accession (Same CDS sequence)	XM_006498014.3 , XM_006498014.4
Accession Version	XM_006498014.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498014.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GCCTTGCTTG GCACAGCCAC TGTCCTTGGA GAGAGGACCA ATGGAGCCCT CTCTTCTGCT 
CTAGATGAGT CTGACTATCA GACCGAGTAT GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA 
ACGTATGCTG ACTTCCAGAG CACTGGGATT GAGCTAGACT CTGACTCGGA ATATGAGCCT 
TCGATGTTGC AAGGACCCCC CAGCTTGACT TCCCCAGAAC AGTCCCAGGA CTCCTTCCCT 
TGGCTCCCTA ATCAAGATGA CCAGGGGCCA AGACTGGAAC ATCCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498077.1
CDS	977..5107
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498014.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498014.3

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GCCTTGCTTG GCACAGCCAC TGTCCTTGGA GAGAGGACCA ATGGAGCCCT CTCTTCTGCT 
CTAGATGAGT CTGACTATCA GACCGAGTAT GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA 
ACGTATGCTG ACTTCCAGAG CACTGGGATT GAGCTAGACT CTGACTCGGA ATATGAGCCT 
TCGATGTTGC AAGGACCCCC CAGCTTGACT TCCCCAGAAC AGTCCCAGGA CTCCTTCCCT 
TGGCTCCCTA ATCAAGATGA CCAGGGGCCA AGACTGGAAC ATCCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498014.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498077.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu29537
Clone ID Related Accession (Same CDS sequence)	XM_006498014.3 , XM_006498014.4
Accession Version	XM_006498014.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498014.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GCCTTGCTTG GCACAGCCAC TGTCCTTGGA GAGAGGACCA ATGGAGCCCT CTCTTCTGCT 
CTAGATGAGT CTGACTATCA GACCGAGTAT GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA 
ACGTATGCTG ACTTCCAGAG CACTGGGATT GAGCTAGACT CTGACTCGGA ATATGAGCCT 
TCGATGTTGC AAGGACCCCC CAGCTTGACT TCCCCAGAAC AGTCCCAGGA CTCCTTCCCT 
TGGCTCCCTA ATCAAGATGA CCAGGGGCCA AGACTGGAAC ATCCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498077.1
CDS	295..4425
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498014.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498014.4

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GCCTTGCTTG GCACAGCCAC TGTCCTTGGA GAGAGGACCA ATGGAGCCCT CTCTTCTGCT 
CTAGATGAGT CTGACTATCA GACCGAGTAT GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA 
ACGTATGCTG ACTTCCAGAG CACTGGGATT GAGCTAGACT CTGACTCGGA ATATGAGCCT 
TCGATGTTGC AAGGACCCCC CAGCTTGACT TCCCCAGAAC AGTCCCAGGA CTCCTTCCCT 
TGGCTCCCTA ATCAAGATGA CCAGGGGCCA AGACTGGAAC ATCCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498015.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498078.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X3, mRNA.

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CloneID	OMu29538
Clone ID Related Accession (Same CDS sequence)	XM_006498015.3 , XM_006498015.4
Accession Version	XM_006498015.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498015.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGATG GCACCGAAGT GGTGGTCAAA GAGGTCCTGC CAGGGGACAG TGTCCACAGT 
CTCCTCAGCA TTTTGGATGT GATCACCGGT CACACTGCAC CATACAAAAC AGTTTCTGCC 
CGTGCCGCTG TCTCGTCCAC CGTGCTCTGG CTTCCTGCAG CAGCTTTTCA AGGAGTGTTT 
GAGAAATACC CTGAAACTCT GGTCAGGGTT GTGCAGATCA TCATGGTCCG GCTGCAGCGG 
GTGACTTTCC TGGCTCTGCA CAACTACCTG GGGCTGACTA CAGAGCTCTT CAACCCTGAG 
AGCCAGGCCA TCCCTCTCTT GTCAGTAGCA AGTGTGGCTG GACGAGCCAA GAGACAGATG 
TCATATGGTC CAGAGGAGCA GCTTGAGAGG TCCCTACGGC CCTCGGAGTT CAGCAGCTCA 
GATCATGGGA GCAGCTGTGT GACAGTCTCT GGGCCTCTGC TGAAGAGGAG CTGCTCAGTG 
CCTCTGCCTT CTAACCATGG GGAGGTGGAT GAGCTCAGAC AGTCCCAGGG AAGTGGCTCT 
AACACTTCTG CCTTCCAAGA AAGCCATGAG GGAGCCACCT CAGACCTGGG GATGGCATAC 
AACCGTGCCA GGATTTTGCC ACACTCTGAT GAGCAACTTG GGAACTCTTT GGCCAGCAAG 
TCCAAGAAAA GTGTTGTCGC AGAGACCCCC TCTGCCATCT TCCACTACTC AGAAAATTTC 
AGGGATGAGA CTGGGGCCTG TGGGAAGACA GATGCTATCT TCAGAGCTGC CACAAAGGAC 
CTCCTTACCC TGATGAAGCT AGATGACCCA TCCCTGTTGG ATGGCCGGGT GGCATTTCTT 
CATGTTCCTG CAGGCACCTT AGTGTCAAAA CAGGGAGATC AGGATGTCAA TATTTTGTTT 
GTGGTCTCGG GGATGCTGCA TGTGTACCAG CAAAAGATTG ACAGCTTGGA GGATACTTGC 
CTGTTCCTCA CACATCCCGG GGAGATGGTG GGTCAGCTGG CGGTGCTCAC TGGAGAACCC 
CTAATGTTCA CCATCAGAGC CAACCGAGAC TGCAGCTTTT TGTCCATCTC GAAGGCCCAC 
TTCTATGAGA TCATGAGGAA ACGGCCGGAT GTTGTCCTGG GCGTGGCACA CACAGTTGTG 
AAGAGGATGT CATCCTTTGT ACGGCAGATT GATTTTGCTC TAGACTGGAT GGAAGTAGAG 
GCTGGACGTG CCATATACAG GCAAGGAGAC AAGTCTGACT GCACATACAT CGTTCTCAGT 
GGCAGGCTGC GTTCTGTCAT CCGAAAAGAT GATGGGAAAA AACGCCTGGC AGGGGAATAT 
GGCCGAGGAG ACCTTGTTGG TGTGGTGGAG ACGCTGACCC ACCAGGCCAG AGCAACCACA 
GTGCATGCTG TCCGGGACTC AGAACTGGCT AAGCTGCCAG CAGGAGCTCT GACGTCTATC 
AAGCGCAGGT ACCCACAGGT TGTAACGCGA CTGATTCATC TCTTGGGCGA GAAGATCTTA 
GGCAGTCTCC AGCAGGGATC TGCAACAGGT CACCAGCTTG GATTTAACAC AGCAAGCAGC 
AAATGGGACT TGGGGAACCC GCCTGGCAAT CTGTCCACAG TGGCAGCACT GCCTGCTTCT 
GAGGATGTGC CCCTAACCGC CTTTGCTCTG GAGCTCCAAC ATGCCCTCAG TGCCATCGGT 
CCTGTCCTGC TGCTGACCAG TGACAACATC AAACAACGCC TGGGTTCCGC TGCTCTAGAC 
AGCATCCATG AGTACCGGCT CTCCAGTTGG CTGGGCCAGC AGGAGGACAT CCACCGGATT 
GTGCTGTATC AGGCAGACGG CACACTCACA CCGTGGACCC AGCGCTGCAT CCGGCAGGCT 
GACTGCATCC TCATCGTGGG CTTGGGTGAG CAAGAGCCAG CAGTAGGTGA GCTGGAGCAG 
ATGTTGGAGA GCACAGCTGT GCGTGCCCAG AAGCAGCTAA TCTTGCTGCA CAAAGAGGAC 
GGTCCAGTGC CTTCCCGCAC AGTGGAGTGG CTCAACATGC GAAGTTGGTG TTCTGGCCAT 
TTGCACCTCT GCTGCCCACG CCGTGTCTTC TCCAAGAGAA GCTTGCCCAA GCTGGTCGAG 
ATGTACACAC GTGTTTTCCA ACGACCACCA GACCGTCATT CTGATTTTTC CCGCCTGGCA 
CGGATGCTGA CAGGCAATGC CATTGCCTTG GTGCTTGGAG GGGGTGGAGC TAGAGGGTGT 
GCCCAGGTGG GCATCCTCAG GGCACTAGCA GAGTGTGGCG TCCCCGTGGA CATAATCGGA 
GGCACATCCA TCGGGGCTTT CATGGGTGCT CTGTTTGCTG AGGAGCGGAG CTACAGCCAA 
ACACGGATCC GGGCCAAGCA GTGGGCAGAG GGTATGACAT CAATGATGAA GACTATACTG 
GACCTGACCT ACCCAATCAC ATCCATGTTC TCTGGCACTG GCTTCAATAG CAGCATCAGC 
AACATCTTCA AAGACCGGCA GATTGAGGAC CTGTGGCTCC CCTACTTTGC CATCACCACC 
GACATCACGG CCTCTGCCAT GCGGGTTCAC ACAGATGGCT CTCTGTGGAG GTATGTCCGT 
GCAAGCATGT CCTTGTCAGG CTATATGCCC CCACTCTGCG ACCCCAAGGA TGGACACCTG 
CTGATGGATG GTGGTTACAT CAACAACCTC CCAGCGGATG TGGCAAGATC CATGGGGGCA 
AAGGTTGTGA TTGCCATCGA TGTTGGCAGC CGGGACGAGA CAGACCTCAC CAACTATGGC 
GATGCCTTGT CTGGATGGTG GCTGCTTTGG AAACGCTGGA ACCCCTTGGC TACAAAAGTC 
AAGGTGTTGA ACATGGCAGA GATTCAGACT CGACTGGCCT ATGTGTGCTG CGTCCGGCAG 
CTGGAGATGG TGAAGAACAG CGACTACTGT GAGTACCTGA GGCCTCCCAT CGACAGTTAC 
CGCACCCTGG ACTTCGGCAA ATTTGATGAG ATCTGTGAAG TTGGGTATCA ACATGGGCGG 
ACAGTGTTTG ACATCTGGGT CCGGAGTGGT GTCCTAGAGA AGATGTTGCA GGACCAGCAG 
GGGACAAGCA AGAGGAAGGA CTGTGGGGTG TTCACCTGCC CCAATTCCTC CTTCACGGAC 
CTTGCTGAGA TTGTGTCACG GATTGAGCCG GCCAAGGTGG CTGCAGTGGA TGCCTTGCTT 
GGCACAGCCA CTGTCCTTGG AGAGAGGACC AATGGAGCCC TCTCTTCTGC TCTAGATGAG 
TCTGACTATC AGACCGAGTA TGAGGAGGAA CTGCCAGCCA TCCCCAAGGA AACGTATGCT 
GACTTCCAGA GCACTGGGAT TGAGCTAGAC TCTGACTCGG AATATGAGCC TTCGATGTTG 
CAAGGACCCC CCAGCTTGAC TTCCCCAGAA CAGTCCCAGG ACTCCTTCCC TTGGCTCCCT 
AATCAAGATG ACCAGGGGCC AAGACTGGAA CATCCTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498078.1
CDS	295..4416
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDADGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498015.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498015.4

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGATG GCACCGAAGT GGTGGTCAAA GAGGTCCTGC CAGGGGACAG TGTCCACAGT 
CTCCTCAGCA TTTTGGATGT GATCACCGGT CACACTGCAC CATACAAAAC AGTTTCTGCC 
CGTGCCGCTG TCTCGTCCAC CGTGCTCTGG CTTCCTGCAG CAGCTTTTCA AGGAGTGTTT 
GAGAAATACC CTGAAACTCT GGTCAGGGTT GTGCAGATCA TCATGGTCCG GCTGCAGCGG 
GTGACTTTCC TGGCTCTGCA CAACTACCTG GGGCTGACTA CAGAGCTCTT CAACCCTGAG 
AGCCAGGCCA TCCCTCTCTT GTCAGTAGCA AGTGTGGCTG GACGAGCCAA GAGACAGATG 
TCATATGGTC CAGAGGAGCA GCTTGAGAGG TCCCTACGGC CCTCGGAGTT CAGCAGCTCA 
GATCATGGGA GCAGCTGTGT GACAGTCTCT GGGCCTCTGC TGAAGAGGAG CTGCTCAGTG 
CCTCTGCCTT CTAACCATGG GGAGGTGGAT GAGCTCAGAC AGTCCCAGGG AAGTGGCTCT 
AACACTTCTG CCTTCCAAGA AAGCCATGAG GGAGCCACCT CAGACCTGGG GATGGCATAC 
AACCGTGCCA GGATTTTGCC ACACTCTGAT GAGCAACTTG GGAACTCTTT GGCCAGCAAG 
TCCAAGAAAA GTGTTGTCGC AGAGACCCCC TCTGCCATCT TCCACTACTC AGAAAATTTC 
AGGGATGAGA CTGGGGCCTG TGGGAAGACA GATGCTATCT TCAGAGCTGC CACAAAGGAC 
CTCCTTACCC TGATGAAGCT AGATGACCCA TCCCTGTTGG ATGGCCGGGT GGCATTTCTT 
CATGTTCCTG CAGGCACCTT AGTGTCAAAA CAGGGAGATC AGGATGTCAA TATTTTGTTT 
GTGGTCTCGG GGATGCTGCA TGTGTACCAG CAAAAGATTG ACAGCTTGGA GGATACTTGC 
CTGTTCCTCA CACATCCCGG GGAGATGGTG GGTCAGCTGG CGGTGCTCAC TGGAGAACCC 
CTAATGTTCA CCATCAGAGC CAACCGAGAC TGCAGCTTTT TGTCCATCTC GAAGGCCCAC 
TTCTATGAGA TCATGAGGAA ACGGCCGGAT GTTGTCCTGG GCGTGGCACA CACAGTTGTG 
AAGAGGATGT CATCCTTTGT ACGGCAGATT GATTTTGCTC TAGACTGGAT GGAAGTAGAG 
GCTGGACGTG CCATATACAG GCAAGGAGAC AAGTCTGACT GCACATACAT CGTTCTCAGT 
GGCAGGCTGC GTTCTGTCAT CCGAAAAGAT GATGGGAAAA AACGCCTGGC AGGGGAATAT 
GGCCGAGGAG ACCTTGTTGG TGTGGTGGAG ACGCTGACCC ACCAGGCCAG AGCAACCACA 
GTGCATGCTG TCCGGGACTC AGAACTGGCT AAGCTGCCAG CAGGAGCTCT GACGTCTATC 
AAGCGCAGGT ACCCACAGGT TGTAACGCGA CTGATTCATC TCTTGGGCGA GAAGATCTTA 
GGCAGTCTCC AGCAGGGATC TGCAACAGGT CACCAGCTTG GATTTAACAC AGCAAGCAGC 
AAATGGGACT TGGGGAACCC GCCTGGCAAT CTGTCCACAG TGGCAGCACT GCCTGCTTCT 
GAGGATGTGC CCCTAACCGC CTTTGCTCTG GAGCTCCAAC ATGCCCTCAG TGCCATCGGT 
CCTGTCCTGC TGCTGACCAG TGACAACATC AAACAACGCC TGGGTTCCGC TGCTCTAGAC 
AGCATCCATG AGTACCGGCT CTCCAGTTGG CTGGGCCAGC AGGAGGACAT CCACCGGATT 
GTGCTGTATC AGGCAGACGG CACACTCACA CCGTGGACCC AGCGCTGCAT CCGGCAGGCT 
GACTGCATCC TCATCGTGGG CTTGGGTGAG CAAGAGCCAG CAGTAGGTGA GCTGGAGCAG 
ATGTTGGAGA GCACAGCTGT GCGTGCCCAG AAGCAGCTAA TCTTGCTGCA CAAAGAGGAC 
GGTCCAGTGC CTTCCCGCAC AGTGGAGTGG CTCAACATGC GAAGTTGGTG TTCTGGCCAT 
TTGCACCTCT GCTGCCCACG CCGTGTCTTC TCCAAGAGAA GCTTGCCCAA GCTGGTCGAG 
ATGTACACAC GTGTTTTCCA ACGACCACCA GACCGTCATT CTGATTTTTC CCGCCTGGCA 
CGGATGCTGA CAGGCAATGC CATTGCCTTG GTGCTTGGAG GGGGTGGAGC TAGAGGGTGT 
GCCCAGGTGG GCATCCTCAG GGCACTAGCA GAGTGTGGCG TCCCCGTGGA CATAATCGGA 
GGCACATCCA TCGGGGCTTT CATGGGTGCT CTGTTTGCTG AGGAGCGGAG CTACAGCCAA 
ACACGGATCC GGGCCAAGCA GTGGGCAGAG GGTATGACAT CAATGATGAA GACTATACTG 
GACCTGACCT ACCCAATCAC ATCCATGTTC TCTGGCACTG GCTTCAATAG CAGCATCAGC 
AACATCTTCA AAGACCGGCA GATTGAGGAC CTGTGGCTCC CCTACTTTGC CATCACCACC 
GACATCACGG CCTCTGCCAT GCGGGTTCAC ACAGATGGCT CTCTGTGGAG GTATGTCCGT 
GCAAGCATGT CCTTGTCAGG CTATATGCCC CCACTCTGCG ACCCCAAGGA TGGACACCTG 
CTGATGGATG GTGGTTACAT CAACAACCTC CCAGCGGATG TGGCAAGATC CATGGGGGCA 
AAGGTTGTGA TTGCCATCGA TGTTGGCAGC CGGGACGAGA CAGACCTCAC CAACTATGGC 
GATGCCTTGT CTGGATGGTG GCTGCTTTGG AAACGCTGGA ACCCCTTGGC TACAAAAGTC 
AAGGTGTTGA ACATGGCAGA GATTCAGACT CGACTGGCCT ATGTGTGCTG CGTCCGGCAG 
CTGGAGATGG TGAAGAACAG CGACTACTGT GAGTACCTGA GGCCTCCCAT CGACAGTTAC 
CGCACCCTGG ACTTCGGCAA ATTTGATGAG ATCTGTGAAG TTGGGTATCA ACATGGGCGG 
ACAGTGTTTG ACATCTGGGT CCGGAGTGGT GTCCTAGAGA AGATGTTGCA GGACCAGCAG 
GGGACAAGCA AGAGGAAGGA CTGTGGGGTG TTCACCTGCC CCAATTCCTC CTTCACGGAC 
CTTGCTGAGA TTGTGTCACG GATTGAGCCG GCCAAGGTGG CTGCAGTGGA TGCCTTGCTT 
GGCACAGCCA CTGTCCTTGG AGAGAGGACC AATGGAGCCC TCTCTTCTGC TCTAGATGAG 
TCTGACTATC AGACCGAGTA TGAGGAGGAA CTGCCAGCCA TCCCCAAGGA AACGTATGCT 
GACTTCCAGA GCACTGGGAT TGAGCTAGAC TCTGACTCGG AATATGAGCC TTCGATGTTG 
CAAGGACCCC CCAGCTTGAC TTCCCCAGAA CAGTCCCAGG ACTCCTTCCC TTGGCTCCCT 
AATCAAGATG ACCAGGGGCC AAGACTGGAA CATCCTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498016.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498079.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X4, mRNA.

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CloneID	OMu29539
Clone ID Related Accession (Same CDS sequence)	XM_006498016.4 , XM_006498016.3
Accession Version	XM_006498016.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498016.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGAATA TGAGCCTTCG 
ATGTTGCAAG GACCCCCCAG CTTGACTTCC CCAGAACAGT CCCAGGACTC CTTCCCTTGG 
CTCCCTAATC AAGATGACCA GGGGCCAAGA CTGGAACATC CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498079.1
CDS	295..4362
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498016.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498016.4

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGAATA TGAGCCTTCG 
ATGTTGCAAG GACCCCCCAG CTTGACTTCC CCAGAACAGT CCCAGGACTC CTTCCCTTGG 
CTCCCTAATC AAGATGACCA GGGGCCAAGA CTGGAACATC CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498018.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498081.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu29540
Clone ID Related Accession (Same CDS sequence)	XM_006498018.4 , XM_006498019.3 , XM_006498017.4 , XM_006498019.4 , XM_006498018.3 , XM_006498017.3
Accession Version	XM_006498018.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498018.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498081.1
CDS	373..4425
Translation	MEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498018.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498018.4

ORF Insert Sequence:

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498017.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498080.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X5, mRNA.

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CloneID	OMu29540
Clone ID Related Accession (Same CDS sequence)	XM_006498018.4 , XM_006498019.3 , XM_006498017.4 , XM_006498019.4 , XM_006498018.3 , XM_006498017.3
Accession Version	XM_006498017.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498017.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498080.1
CDS	469..4521
Translation	MEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498017.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498017.4

ORF Insert Sequence:

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498019.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498082.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X7, mRNA.

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CloneID	OMu29540
Clone ID Related Accession (Same CDS sequence)	XM_006498018.4 , XM_006498019.3 , XM_006498017.4 , XM_006498019.4 , XM_006498018.3 , XM_006498017.3
Accession Version	XM_006498019.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498019.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498082.1
CDS	277..4329
Translation	MEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498019.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498019.4

ORF Insert Sequence:

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498020.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498083.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X8, mRNA.

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CloneID	OMu29541
Clone ID Related Accession (Same CDS sequence)	XM_006498020.4 , XM_006498020.3
Accession Version	XM_006498020.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498020.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GCCTTGCTTG GCACAGCCAC TGTCCTTGGA GAGAGGACCA ATGGAGCCCT CTCTTCTGCT 
CTAGATGAGT CTGACTATCA GACCGAGTAT GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA 
ACGTATGCTG ACTTCCAGAG CACTGGGATT GAGCTAGACT CTGACTCGGT GAGGAATATG 
AGCCTTCGAT GTTGCAAGGA CCCCCCAGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498083.1
CDS	295..4347
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSVRNMSLRCCKDPPA

Target ORF information:

RefSeq Version	XM_006498020.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498020.4

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GCCTTGCTTG GCACAGCCAC TGTCCTTGGA GAGAGGACCA ATGGAGCCCT CTCTTCTGCT 
CTAGATGAGT CTGACTATCA GACCGAGTAT GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA 
ACGTATGCTG ACTTCCAGAG CACTGGGATT GAGCTAGACT CTGACTCGGT GAGGAATATG 
AGCCTTCGAT GTTGCAAGGA CCCCCCAGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498021.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498084.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu29542
Clone ID Related Accession (Same CDS sequence)	XM_006498021.3 , XM_006498021.4
Accession Version	XM_006498021.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498021.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGTGAG GAATATGAGC 
CTTCGATGTT GCAAGGACCC CCCAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498084.1
CDS	295..4284
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSVRNMSLRCCKDPPA

Target ORF information:

RefSeq Version	XM_006498021.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498021.4

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGTGAG GAATATGAGC 
CTTCGATGTT GCAAGGACCC CCCAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498022.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498085.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X13, mRNA.

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CloneID	OMu29543
Clone ID Related Accession (Same CDS sequence)	XM_006498022.3 , XM_006498022.2
Accession Version	XM_006498022.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498022.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTATG GCCGGAAGAT CATGAGGAAG GTGACCACAC TCCCCCATAC CTTGGTTGGG 
AATACCTCTG CACCCCGGCA GCGAGTCAGG AAGAGGACCA AAGTGCTGTC TTTAGCCAAG 
AGGATTCTGC GCTTTAAGAA AGAGTATCCC ACTTTGCAGC CAAAAGAGCC TCCGCCCTCC 
CTGCTTGAGG CTGATCTTAC AGAGTTTGAT GTGAAGAACT CTCATCTGCC ATCAGAAGTC 
TTGTACATGT TGAAAAATGT TCGGGTTCTA GGCCACTTTG AGAAGCCACT ATTTCTGGAA 
CTTTGCAAAC ATATGGTCTT TGTGCAGCTA CAAGAAGGGG AGCACGTATT CCAACCTGGG 
GAGCCTGACA TCAGCATCTA TGTGGTTCAG GACGGACGGC TGGAGGTCTG CATCCAAGAC 
GCTGTGACAC AGGATGGCAC CGAAGTGGTG GTCAAAGAGG TCCTGCCAGG GGACAGTGTC 
CACAGTCTCC TCAGCATTTT GGATGTGATC ACCGGTCACA CTGCACCATA CAAAACAGTT 
TCTGCCCGTG CCGCTGTCTC GTCCACCGTG CTCTGGCTTC CTGCAGCAGC TTTTCAAGGA 
GTGTTTGAGA AATACCCTGA AACTCTGGTC AGGGTTGTGC AGATCATCAT GGTCCGGCTG 
CAGCGGGTGA CTTTCCTGGC TCTGCACAAC TACCTGGGGC TGACTACAGA GCTCTTCAAC 
CCTGAGAGCC AGGCCATCCC TCTCTTGTCA GTAGCAAGTG TGGCTGGACG AGCCAAGAGA 
CAGATGTCAT ATGGTCCAGA GGAGCAGCTT GAGAGGTCCC TACGGCCCTC GGAGTTCAGC 
AGCTCAGATC ATGGGAGCAG CTGTGTGACA GTCTCTGGGC CTCTGCTGAA GAGGAGCTGC 
TCAGTGCCTC TGCCTTCTAA CCATGGGGAG GTGGATGAGC TCAGACAGTC CCAGGGAAGT 
GGCTCTAACA CTTCTGCCTT CCAAGAAAGC CATGAGGGAG CCACCTCAGA CCTGGGGATG 
GCATACAACC GTGCCAGGAT TTTGCCACAC TCTGATGAGC AACTTGGGAA CTCTTTGGCC 
AGCAAGTCCA AGAAAAGTGT TGTCGCAGAG ACCCCCTCTG CCATCTTCCA CTACTCAGAA 
AATTTCAGGG ATGAGACTGG GGCCTGTGGG AAGACAGATG CTATCTTCAG AGCTGCCACA 
AAGGACCTCC TTACCCTGAT GAAGCTAGAT GACCCATCCC TGTTGGATGG CCGGGTGGCA 
TTTCTTCATG TTCCTGCAGG CACCTTAGTG TCAAAACAGG GAGATCAGGA TGTCAATATT 
TTGTTTGTGG TCTCGGGGAT GCTGCATGTG TACCAGCAAA AGATTGACAG CTTGGAGGAT 
ACTTGCCTGT TCCTCACACA TCCCGGGGAG ATGGTGGGTC AGCTGGCGGT GCTCACTGGA 
GAACCCCTAA TGTTCACCAT CAGAGCCAAC CGAGACTGCA GCTTTTTGTC CATCTCGAAG 
GCCCACTTCT ATGAGATCAT GAGGAAACGG CCGGATGTTG TCCTGGGCGT GGCACACACA 
GTTGTGAAGA GGATGTCATC CTTTGTACGG CAGATTGATT TTGCTCTAGA CTGGATGGAA 
GTAGAGGCTG GACGTGCCAT ATACAGGCAA GGAGACAAGT CTGACTGCAC ATACATCGTT 
CTCAGTGGCA GGCTGCGTTC TGTCATCCGA AAAGATGATG GGAAAAAACG CCTGGCAGGG 
GAATATGGCC GAGGAGACCT TGTTGGTGTG GTGGAGACGC TGACCCACCA GGCCAGAGCA 
ACCACAGTGC ATGCTGTCCG GGACTCAGAA CTGGCTAAGC TGCCAGCAGG AGCTCTGACG 
TCTATCAAGC GCAGGTACCC ACAGGTTGTA ACGCGACTGA TTCATCTCTT GGGCGAGAAG 
ATCTTAGGCA GTCTCCAGCA GGGATCTGCA ACAGGTCACC AGCTTGGATT TAACACAGCA 
AGCAGCAAAT GGGACTTGGG GAACCCGCCT GGCAATCTGT CCACAGTGGC AGCACTGCCT 
GCTTCTGAGG ATGTGCCCCT AACCGCCTTT GCTCTGGAGC TCCAACATGC CCTCAGTGCC 
ATCGGTCCTG TCCTGCTGCT GACCAGTGAC AACATCAAAC AACGCCTGGG TTCCGCTGCT 
CTAGACAGCA TCCATGAGTA CCGGCTCTCC AGTTGGCTGG GCCAGCAGGA GGACATCCAC 
CGGATTGTGC TGTATCAGGC AGACGGCACA CTCACACCGT GGACCCAGCG CTGCATCCGG 
CAGGCTGACT GCATCCTCAT CGTGGGCTTG GGTGAGCAAG AGCCAGCAGT AGGTGAGCTG 
GAGCAGATGT TGGAGAGCAC AGCTGTGCGT GCCCAGAAGC AGCTAATCTT GCTGCACAAA 
GAGGACGGTC CAGTGCCTTC CCGCACAGTG GAGTGGCTCA ACATGCGAAG TTGGTGTTCT 
GGCCATTTGC ACCTCTGCTG CCCACGCCGT GTCTTCTCCA AGAGAAGCTT GCCCAAGCTG 
GTCGAGATGT ACACACGTGT TTTCCAACGA CCACCAGACC GTCATTCTGA TTTTTCCCGC 
CTGGCACGGA TGCTGACAGG CAATGCCATT GCCTTGGTGC TTGGAGGGGG TGGAGCTAGA 
GGGTGTGCCC AGGTGGGCAT CCTCAGGGCA CTAGCAGAGT GTGGCGTCCC CGTGGACATA 
ATCGGAGGCA CATCCATCGG GGCTTTCATG GGTGCTCTGT TTGCTGAGGA GCGGAGCTAC 
AGCCAAACAC GGATCCGGGC CAAGCAGTGG GCAGAGGGTA TGACATCAAT GATGAAGACT 
ATACTGGACC TGACCTACCC AATCACATCC ATGTTCTCTG GCACTGGCTT CAATAGCAGC 
ATCAGCAACA TCTTCAAAGA CCGGCAGATT GAGGACCTGT GGCTCCCCTA CTTTGCCATC 
ACCACCGACA TCACGGCCTC TGCCATGCGG GTTCACACAG ATGGCTCTCT GTGGAGGTAT 
GTCCGTGCAA GCATGTCCTT GTCAGGCTAT ATGCCCCCAC TCTGCGACCC CAAGGATGGA 
CACCTGCTGA TGGATGGTGG TTACATCAAC AACCTCCCAG CGGATGTGGC AAGATCCATG 
GGGGCAAAGG TTGTGATTGC CATCGATGTT GGCAGCCGGG ACGAGACAGA CCTCACCAAC 
TATGGCGATG CCTTGTCTGG ATGGTGGCTG CTTTGGAAAC GCTGGAACCC CTTGGCTACA 
AAAGTCAAGG TGTTGAACAT GGCAGAGATT CAGACTCGAC TGGCCTATGT GTGCTGCGTC 
CGGCAGCTGG AGATGGTGAA GAACAGCGAC TACTGTGAGT ACCTGAGGCC TCCCATCGAC 
AGTTACCGCA CCCTGGACTT CGGCAAATTT GATGAGATCT GTGAAGTTGG GTATCAACAT 
GGGCGGACAG TGTTTGACAT CTGGGTCCGG AGTGGTGTCC TAGAGAAGAT GTTGCAGGAC 
CAGCAGGGGA CAAGCAAGAG GAAGGACTGT GGGGTGTTCA CCTGCCCCAA TTCCTCCTTC 
ACGGACCTTG CTGAGATTGT GTCACGGATT GAGCCGGCCA AGGTGGCTGC AGTGGATGCC 
TTGCTTGGCA CAGCCACTGT CCTTGGAGAG AGGACCAATG GAGCCCTCTC TTCTGCTCTA 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGAATA TGAGCCTTCG 
ATGTTGCAAG GACCCCCCAG CTTGACTTCC CCAGAACAGT CCCAGGACTC CTTCCCTTGG 
CTCCCTAATC AAGATGACCA GGGGCCAAGA CTGGAACATC CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498085.1
CDS	125..4012
Translation	MFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498022.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498022.3

ORF Insert Sequence:

ATGTTTTATG GCCGGAAGAT CATGAGGAAG GTGACCACAC TCCCCCATAC CTTGGTTGGG 
AATACCTCTG CACCCCGGCA GCGAGTCAGG AAGAGGACCA AAGTGCTGTC TTTAGCCAAG 
AGGATTCTGC GCTTTAAGAA AGAGTATCCC ACTTTGCAGC CAAAAGAGCC TCCGCCCTCC 
CTGCTTGAGG CTGATCTTAC AGAGTTTGAT GTGAAGAACT CTCATCTGCC ATCAGAAGTC 
TTGTACATGT TGAAAAATGT TCGGGTTCTA GGCCACTTTG AGAAGCCACT ATTTCTGGAA 
CTTTGCAAAC ATATGGTCTT TGTGCAGCTA CAAGAAGGGG AGCACGTATT CCAACCTGGG 
GAGCCTGACA TCAGCATCTA TGTGGTTCAG GACGGACGGC TGGAGGTCTG CATCCAAGAC 
GCTGTGACAC AGGATGGCAC CGAAGTGGTG GTCAAAGAGG TCCTGCCAGG GGACAGTGTC 
CACAGTCTCC TCAGCATTTT GGATGTGATC ACCGGTCACA CTGCACCATA CAAAACAGTT 
TCTGCCCGTG CCGCTGTCTC GTCCACCGTG CTCTGGCTTC CTGCAGCAGC TTTTCAAGGA 
GTGTTTGAGA AATACCCTGA AACTCTGGTC AGGGTTGTGC AGATCATCAT GGTCCGGCTG 
CAGCGGGTGA CTTTCCTGGC TCTGCACAAC TACCTGGGGC TGACTACAGA GCTCTTCAAC 
CCTGAGAGCC AGGCCATCCC TCTCTTGTCA GTAGCAAGTG TGGCTGGACG AGCCAAGAGA 
CAGATGTCAT ATGGTCCAGA GGAGCAGCTT GAGAGGTCCC TACGGCCCTC GGAGTTCAGC 
AGCTCAGATC ATGGGAGCAG CTGTGTGACA GTCTCTGGGC CTCTGCTGAA GAGGAGCTGC 
TCAGTGCCTC TGCCTTCTAA CCATGGGGAG GTGGATGAGC TCAGACAGTC CCAGGGAAGT 
GGCTCTAACA CTTCTGCCTT CCAAGAAAGC CATGAGGGAG CCACCTCAGA CCTGGGGATG 
GCATACAACC GTGCCAGGAT TTTGCCACAC TCTGATGAGC AACTTGGGAA CTCTTTGGCC 
AGCAAGTCCA AGAAAAGTGT TGTCGCAGAG ACCCCCTCTG CCATCTTCCA CTACTCAGAA 
AATTTCAGGG ATGAGACTGG GGCCTGTGGG AAGACAGATG CTATCTTCAG AGCTGCCACA 
AAGGACCTCC TTACCCTGAT GAAGCTAGAT GACCCATCCC TGTTGGATGG CCGGGTGGCA 
TTTCTTCATG TTCCTGCAGG CACCTTAGTG TCAAAACAGG GAGATCAGGA TGTCAATATT 
TTGTTTGTGG TCTCGGGGAT GCTGCATGTG TACCAGCAAA AGATTGACAG CTTGGAGGAT 
ACTTGCCTGT TCCTCACACA TCCCGGGGAG ATGGTGGGTC AGCTGGCGGT GCTCACTGGA 
GAACCCCTAA TGTTCACCAT CAGAGCCAAC CGAGACTGCA GCTTTTTGTC CATCTCGAAG 
GCCCACTTCT ATGAGATCAT GAGGAAACGG CCGGATGTTG TCCTGGGCGT GGCACACACA 
GTTGTGAAGA GGATGTCATC CTTTGTACGG CAGATTGATT TTGCTCTAGA CTGGATGGAA 
GTAGAGGCTG GACGTGCCAT ATACAGGCAA GGAGACAAGT CTGACTGCAC ATACATCGTT 
CTCAGTGGCA GGCTGCGTTC TGTCATCCGA AAAGATGATG GGAAAAAACG CCTGGCAGGG 
GAATATGGCC GAGGAGACCT TGTTGGTGTG GTGGAGACGC TGACCCACCA GGCCAGAGCA 
ACCACAGTGC ATGCTGTCCG GGACTCAGAA CTGGCTAAGC TGCCAGCAGG AGCTCTGACG 
TCTATCAAGC GCAGGTACCC ACAGGTTGTA ACGCGACTGA TTCATCTCTT GGGCGAGAAG 
ATCTTAGGCA GTCTCCAGCA GGGATCTGCA ACAGGTCACC AGCTTGGATT TAACACAGCA 
AGCAGCAAAT GGGACTTGGG GAACCCGCCT GGCAATCTGT CCACAGTGGC AGCACTGCCT 
GCTTCTGAGG ATGTGCCCCT AACCGCCTTT GCTCTGGAGC TCCAACATGC CCTCAGTGCC 
ATCGGTCCTG TCCTGCTGCT GACCAGTGAC AACATCAAAC AACGCCTGGG TTCCGCTGCT 
CTAGACAGCA TCCATGAGTA CCGGCTCTCC AGTTGGCTGG GCCAGCAGGA GGACATCCAC 
CGGATTGTGC TGTATCAGGC AGACGGCACA CTCACACCGT GGACCCAGCG CTGCATCCGG 
CAGGCTGACT GCATCCTCAT CGTGGGCTTG GGTGAGCAAG AGCCAGCAGT AGGTGAGCTG 
GAGCAGATGT TGGAGAGCAC AGCTGTGCGT GCCCAGAAGC AGCTAATCTT GCTGCACAAA 
GAGGACGGTC CAGTGCCTTC CCGCACAGTG GAGTGGCTCA ACATGCGAAG TTGGTGTTCT 
GGCCATTTGC ACCTCTGCTG CCCACGCCGT GTCTTCTCCA AGAGAAGCTT GCCCAAGCTG 
GTCGAGATGT ACACACGTGT TTTCCAACGA CCACCAGACC GTCATTCTGA TTTTTCCCGC 
CTGGCACGGA TGCTGACAGG CAATGCCATT GCCTTGGTGC TTGGAGGGGG TGGAGCTAGA 
GGGTGTGCCC AGGTGGGCAT CCTCAGGGCA CTAGCAGAGT GTGGCGTCCC CGTGGACATA 
ATCGGAGGCA CATCCATCGG GGCTTTCATG GGTGCTCTGT TTGCTGAGGA GCGGAGCTAC 
AGCCAAACAC GGATCCGGGC CAAGCAGTGG GCAGAGGGTA TGACATCAAT GATGAAGACT 
ATACTGGACC TGACCTACCC AATCACATCC ATGTTCTCTG GCACTGGCTT CAATAGCAGC 
ATCAGCAACA TCTTCAAAGA CCGGCAGATT GAGGACCTGT GGCTCCCCTA CTTTGCCATC 
ACCACCGACA TCACGGCCTC TGCCATGCGG GTTCACACAG ATGGCTCTCT GTGGAGGTAT 
GTCCGTGCAA GCATGTCCTT GTCAGGCTAT ATGCCCCCAC TCTGCGACCC CAAGGATGGA 
CACCTGCTGA TGGATGGTGG TTACATCAAC AACCTCCCAG CGGATGTGGC AAGATCCATG 
GGGGCAAAGG TTGTGATTGC CATCGATGTT GGCAGCCGGG ACGAGACAGA CCTCACCAAC 
TATGGCGATG CCTTGTCTGG ATGGTGGCTG CTTTGGAAAC GCTGGAACCC CTTGGCTACA 
AAAGTCAAGG TGTTGAACAT GGCAGAGATT CAGACTCGAC TGGCCTATGT GTGCTGCGTC 
CGGCAGCTGG AGATGGTGAA GAACAGCGAC TACTGTGAGT ACCTGAGGCC TCCCATCGAC 
AGTTACCGCA CCCTGGACTT CGGCAAATTT GATGAGATCT GTGAAGTTGG GTATCAACAT 
GGGCGGACAG TGTTTGACAT CTGGGTCCGG AGTGGTGTCC TAGAGAAGAT GTTGCAGGAC 
CAGCAGGGGA CAAGCAAGAG GAAGGACTGT GGGGTGTTCA CCTGCCCCAA TTCCTCCTTC 
ACGGACCTTG CTGAGATTGT GTCACGGATT GAGCCGGCCA AGGTGGCTGC AGTGGATGCC 
TTGCTTGGCA CAGCCACTGT CCTTGGAGAG AGGACCAATG GAGCCCTCTC TTCTGCTCTA 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGAATA TGAGCCTTCG 
ATGTTGCAAG GACCCCCCAG CTTGACTTCC CCAGAACAGT CCCAGGACTC CTTCCCTTGG 
CTCCCTAATC AAGATGACCA GGGGCCAAGA CTGGAACATC CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498023.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498086.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X14, mRNA.

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CloneID	OMu29544
Clone ID Related Accession (Same CDS sequence)	XM_006498023.3 , XM_006498023.4
Accession Version	XM_006498023.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498023.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTATG GCCGGAAGAT CATGAGGAAG GTGACCACAC TCCCCCATAC CTTGGTTGGG 
AATACCTCTG CACCCCGGCA GCGAGTCAGG AAGAGGACCA AAGTGCTGTC TTTAGCCAAG 
AGGATTCTGC GCTTTAAGAA AGAGTATCCC ACTTTGCAGC CAAAAGAGCC TCCGCCCTCC 
CTGCTTGAGG CTGATCTTAC AGAGTTTGAT GTGAAGAACT CTCATCTGCC ATCAGAAGTC 
TTGTACATGT TGAAAAATGT TCGGGTTCTA GGCCACTTTG AGAAGCCACT ATTTCTGGAA 
CTTTGCAAAC ATATGGTCTT TGTGCAGCTA CAAGAAGGGG AGCACGTATT CCAACCTGGG 
GAGCCTGACA TCAGCATCTA TGTGGTTCAG GACGGACGGC TGGAGGTCTG CATCCAAGAC 
GCTGATGGCA CCGAAGTGGT GGTCAAAGAG GTCCTGCCAG GGGACAGTGT CCACAGTCTC 
CTCAGCATTT TGGATGTGAT CACCGGTCAC ACTGCACCAT ACAAAACAGT TTCTGCCCGT 
GCCGCTGTCT CGTCCACCGT GCTCTGGCTT CCTGCAGCAG CTTTTCAAGG AGTGTTTGAG 
AAATACCCTG AAACTCTGGT CAGGGTTGTG CAGATCATCA TGGTCCGGCT GCAGCGGGTG 
ACTTTCCTGG CTCTGCACAA CTACCTGGGG CTGACTACAG AGCTCTTCAA CCCTGAGAGC 
CAGGCCATCC CTCTCTTGTC AGTAGCAAGT GTGGCTGGAC GAGCCAAGAG ACAGATGTCA 
TATGGTCCAG AGGAGCAGCT TGAGAGGTCC CTACGGCCCT CGGAGTTCAG CAGCTCAGAT 
CATGGGAGCA GCTGTGTGAC AGTCTCTGGG CCTCTGCTGA AGAGGAGCTG CTCAGTGCCT 
CTGCCTTCTA ACCATGGGGA GGTGGATGAG CTCAGACAGT CCCAGGGAAG TGGCTCTAAC 
ACTTCTGCCT TCCAAGAAAG CCATGAGGGA GCCACCTCAG ACCTGGGGAT GGCATACAAC 
CGTGCCAGGA TTTTGCCACA CTCTGATGAG CAACTTGGGA ACTCTTTGGC CAGCAAGTCC 
AAGAAAAGTG TTGTCGCAGA GACCCCCTCT GCCATCTTCC ACTACTCAGA AAATTTCAGG 
GATGAGACTG GGGCCTGTGG GAAGACAGAT GCTATCTTCA GAGCTGCCAC AAAGGACCTC 
CTTACCCTGA TGAAGCTAGA TGACCCATCC CTGTTGGATG GCCGGGTGGC ATTTCTTCAT 
GTTCCTGCAG GCACCTTAGT GTCAAAACAG GGAGATCAGG ATGTCAATAT TTTGTTTGTG 
GTCTCGGGGA TGCTGCATGT GTACCAGCAA AAGATTGACA GCTTGGAGGA TACTTGCCTG 
TTCCTCACAC ATCCCGGGGA GATGGTGGGT CAGCTGGCGG TGCTCACTGG AGAACCCCTA 
ATGTTCACCA TCAGAGCCAA CCGAGACTGC AGCTTTTTGT CCATCTCGAA GGCCCACTTC 
TATGAGATCA TGAGGAAACG GCCGGATGTT GTCCTGGGCG TGGCACACAC AGTTGTGAAG 
AGGATGTCAT CCTTTGTACG GCAGATTGAT TTTGCTCTAG ACTGGATGGA AGTAGAGGCT 
GGACGTGCCA TATACAGGCA AGGAGACAAG TCTGACTGCA CATACATCGT TCTCAGTGGC 
AGGCTGCGTT CTGTCATCCG AAAAGATGAT GGGAAAAAAC GCCTGGCAGG GGAATATGGC 
CGAGGAGACC TTGTTGGTGT GGTGGAGACG CTGACCCACC AGGCCAGAGC AACCACAGTG 
CATGCTGTCC GGGACTCAGA ACTGGCTAAG CTGCCAGCAG GAGCTCTGAC GTCTATCAAG 
CGCAGGTACC CACAGGTTGT AACGCGACTG ATTCATCTCT TGGGCGAGAA GATCTTAGGC 
AGTCTCCAGC AGGGATCTGC AACAGGTCAC CAGCTTGGAT TTAACACAGC AAGCAGCAAA 
TGGGACTTGG GGAACCCGCC TGGCAATCTG TCCACAGTGG CAGCACTGCC TGCTTCTGAG 
GATGTGCCCC TAACCGCCTT TGCTCTGGAG CTCCAACATG CCCTCAGTGC CATCGGTCCT 
GTCCTGCTGC TGACCAGTGA CAACATCAAA CAACGCCTGG GTTCCGCTGC TCTAGACAGC 
ATCCATGAGT ACCGGCTCTC CAGTTGGCTG GGCCAGCAGG AGGACATCCA CCGGATTGTG 
CTGTATCAGG CAGACGGCAC ACTCACACCG TGGACCCAGC GCTGCATCCG GCAGGCTGAC 
TGCATCCTCA TCGTGGGCTT GGGTGAGCAA GAGCCAGCAG TAGGTGAGCT GGAGCAGATG 
TTGGAGAGCA CAGCTGTGCG TGCCCAGAAG CAGCTAATCT TGCTGCACAA AGAGGACGGT 
CCAGTGCCTT CCCGCACAGT GGAGTGGCTC AACATGCGAA GTTGGTGTTC TGGCCATTTG 
CACCTCTGCT GCCCACGCCG TGTCTTCTCC AAGAGAAGCT TGCCCAAGCT GGTCGAGATG 
TACACACGTG TTTTCCAACG ACCACCAGAC CGTCATTCTG ATTTTTCCCG CCTGGCACGG 
ATGCTGACAG GCAATGCCAT TGCCTTGGTG CTTGGAGGGG GTGGAGCTAG AGGGTGTGCC 
CAGGTGGGCA TCCTCAGGGC ACTAGCAGAG TGTGGCGTCC CCGTGGACAT AATCGGAGGC 
ACATCCATCG GGGCTTTCAT GGGTGCTCTG TTTGCTGAGG AGCGGAGCTA CAGCCAAACA 
CGGATCCGGG CCAAGCAGTG GGCAGAGGGT ATGACATCAA TGATGAAGAC TATACTGGAC 
CTGACCTACC CAATCACATC CATGTTCTCT GGCACTGGCT TCAATAGCAG CATCAGCAAC 
ATCTTCAAAG ACCGGCAGAT TGAGGACCTG TGGCTCCCCT ACTTTGCCAT CACCACCGAC 
ATCACGGCCT CTGCCATGCG GGTTCACACA GATGGCTCTC TGTGGAGGTA TGTCCGTGCA 
AGCATGTCCT TGTCAGGCTA TATGCCCCCA CTCTGCGACC CCAAGGATGG ACACCTGCTG 
ATGGATGGTG GTTACATCAA CAACCTCCCA GCGGATGTGG CAAGATCCAT GGGGGCAAAG 
GTTGTGATTG CCATCGATGT TGGCAGCCGG GACGAGACAG ACCTCACCAA CTATGGCGAT 
GCCTTGTCTG GATGGTGGCT GCTTTGGAAA CGCTGGAACC CCTTGGCTAC AAAAGTCAAG 
GTGTTGAACA TGGCAGAGAT TCAGACTCGA CTGGCCTATG TGTGCTGCGT CCGGCAGCTG 
GAGATGGTGA AGAACAGCGA CTACTGTGAG TACCTGAGGC CTCCCATCGA CAGTTACCGC 
ACCCTGGACT TCGGCAAATT TGATGAGATC TGTGAAGTTG GGTATCAACA TGGGCGGACA 
GTGTTTGACA TCTGGGTCCG GAGTGGTGTC CTAGAGAAGA TGTTGCAGGA CCAGCAGGGG 
ACAAGCAAGA GGAAGGACTG TGGGGTGTTC ACCTGCCCCA ATTCCTCCTT CACGGACCTT 
GCTGAGATTG TGTCACGGAT TGAGCCGGCC AAGGTGGCTG CAGTGGATGC CTTGCTTGGC 
ACAGCCACTG TCCTTGGAGA GAGGACCAAT GGAGCCCTCT CTTCTGCTCT AGATGAGTCT 
GACTATCAGA CCGAGTATGA GGAGGAACTG CCAGCCATCC CCAAGGAAAC GTATGCTGAC 
TTCCAGAGCA CTGGGATTGA GCTAGACTCT GACTCGGAAT ATGAGCCTTC GATGTTGCAA 
GGACCCCCCA GCTTGACTTC CCCAGAACAG TCCCAGGACT CCTTCCCTTG GCTCCCTAAT 
CAAGATGACC AGGGGCCAAG ACTGGAACAT CCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498086.1
CDS	375..4253
Translation	MFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDADGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498023.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498023.4

ORF Insert Sequence:

ATGTTTTATG GCCGGAAGAT CATGAGGAAG GTGACCACAC TCCCCCATAC CTTGGTTGGG 
AATACCTCTG CACCCCGGCA GCGAGTCAGG AAGAGGACCA AAGTGCTGTC TTTAGCCAAG 
AGGATTCTGC GCTTTAAGAA AGAGTATCCC ACTTTGCAGC CAAAAGAGCC TCCGCCCTCC 
CTGCTTGAGG CTGATCTTAC AGAGTTTGAT GTGAAGAACT CTCATCTGCC ATCAGAAGTC 
TTGTACATGT TGAAAAATGT TCGGGTTCTA GGCCACTTTG AGAAGCCACT ATTTCTGGAA 
CTTTGCAAAC ATATGGTCTT TGTGCAGCTA CAAGAAGGGG AGCACGTATT CCAACCTGGG 
GAGCCTGACA TCAGCATCTA TGTGGTTCAG GACGGACGGC TGGAGGTCTG CATCCAAGAC 
GCTGATGGCA CCGAAGTGGT GGTCAAAGAG GTCCTGCCAG GGGACAGTGT CCACAGTCTC 
CTCAGCATTT TGGATGTGAT CACCGGTCAC ACTGCACCAT ACAAAACAGT TTCTGCCCGT 
GCCGCTGTCT CGTCCACCGT GCTCTGGCTT CCTGCAGCAG CTTTTCAAGG AGTGTTTGAG 
AAATACCCTG AAACTCTGGT CAGGGTTGTG CAGATCATCA TGGTCCGGCT GCAGCGGGTG 
ACTTTCCTGG CTCTGCACAA CTACCTGGGG CTGACTACAG AGCTCTTCAA CCCTGAGAGC 
CAGGCCATCC CTCTCTTGTC AGTAGCAAGT GTGGCTGGAC GAGCCAAGAG ACAGATGTCA 
TATGGTCCAG AGGAGCAGCT TGAGAGGTCC CTACGGCCCT CGGAGTTCAG CAGCTCAGAT 
CATGGGAGCA GCTGTGTGAC AGTCTCTGGG CCTCTGCTGA AGAGGAGCTG CTCAGTGCCT 
CTGCCTTCTA ACCATGGGGA GGTGGATGAG CTCAGACAGT CCCAGGGAAG TGGCTCTAAC 
ACTTCTGCCT TCCAAGAAAG CCATGAGGGA GCCACCTCAG ACCTGGGGAT GGCATACAAC 
CGTGCCAGGA TTTTGCCACA CTCTGATGAG CAACTTGGGA ACTCTTTGGC CAGCAAGTCC 
AAGAAAAGTG TTGTCGCAGA GACCCCCTCT GCCATCTTCC ACTACTCAGA AAATTTCAGG 
GATGAGACTG GGGCCTGTGG GAAGACAGAT GCTATCTTCA GAGCTGCCAC AAAGGACCTC 
CTTACCCTGA TGAAGCTAGA TGACCCATCC CTGTTGGATG GCCGGGTGGC ATTTCTTCAT 
GTTCCTGCAG GCACCTTAGT GTCAAAACAG GGAGATCAGG ATGTCAATAT TTTGTTTGTG 
GTCTCGGGGA TGCTGCATGT GTACCAGCAA AAGATTGACA GCTTGGAGGA TACTTGCCTG 
TTCCTCACAC ATCCCGGGGA GATGGTGGGT CAGCTGGCGG TGCTCACTGG AGAACCCCTA 
ATGTTCACCA TCAGAGCCAA CCGAGACTGC AGCTTTTTGT CCATCTCGAA GGCCCACTTC 
TATGAGATCA TGAGGAAACG GCCGGATGTT GTCCTGGGCG TGGCACACAC AGTTGTGAAG 
AGGATGTCAT CCTTTGTACG GCAGATTGAT TTTGCTCTAG ACTGGATGGA AGTAGAGGCT 
GGACGTGCCA TATACAGGCA AGGAGACAAG TCTGACTGCA CATACATCGT TCTCAGTGGC 
AGGCTGCGTT CTGTCATCCG AAAAGATGAT GGGAAAAAAC GCCTGGCAGG GGAATATGGC 
CGAGGAGACC TTGTTGGTGT GGTGGAGACG CTGACCCACC AGGCCAGAGC AACCACAGTG 
CATGCTGTCC GGGACTCAGA ACTGGCTAAG CTGCCAGCAG GAGCTCTGAC GTCTATCAAG 
CGCAGGTACC CACAGGTTGT AACGCGACTG ATTCATCTCT TGGGCGAGAA GATCTTAGGC 
AGTCTCCAGC AGGGATCTGC AACAGGTCAC CAGCTTGGAT TTAACACAGC AAGCAGCAAA 
TGGGACTTGG GGAACCCGCC TGGCAATCTG TCCACAGTGG CAGCACTGCC TGCTTCTGAG 
GATGTGCCCC TAACCGCCTT TGCTCTGGAG CTCCAACATG CCCTCAGTGC CATCGGTCCT 
GTCCTGCTGC TGACCAGTGA CAACATCAAA CAACGCCTGG GTTCCGCTGC TCTAGACAGC 
ATCCATGAGT ACCGGCTCTC CAGTTGGCTG GGCCAGCAGG AGGACATCCA CCGGATTGTG 
CTGTATCAGG CAGACGGCAC ACTCACACCG TGGACCCAGC GCTGCATCCG GCAGGCTGAC 
TGCATCCTCA TCGTGGGCTT GGGTGAGCAA GAGCCAGCAG TAGGTGAGCT GGAGCAGATG 
TTGGAGAGCA CAGCTGTGCG TGCCCAGAAG CAGCTAATCT TGCTGCACAA AGAGGACGGT 
CCAGTGCCTT CCCGCACAGT GGAGTGGCTC AACATGCGAA GTTGGTGTTC TGGCCATTTG 
CACCTCTGCT GCCCACGCCG TGTCTTCTCC AAGAGAAGCT TGCCCAAGCT GGTCGAGATG 
TACACACGTG TTTTCCAACG ACCACCAGAC CGTCATTCTG ATTTTTCCCG CCTGGCACGG 
ATGCTGACAG GCAATGCCAT TGCCTTGGTG CTTGGAGGGG GTGGAGCTAG AGGGTGTGCC 
CAGGTGGGCA TCCTCAGGGC ACTAGCAGAG TGTGGCGTCC CCGTGGACAT AATCGGAGGC 
ACATCCATCG GGGCTTTCAT GGGTGCTCTG TTTGCTGAGG AGCGGAGCTA CAGCCAAACA 
CGGATCCGGG CCAAGCAGTG GGCAGAGGGT ATGACATCAA TGATGAAGAC TATACTGGAC 
CTGACCTACC CAATCACATC CATGTTCTCT GGCACTGGCT TCAATAGCAG CATCAGCAAC 
ATCTTCAAAG ACCGGCAGAT TGAGGACCTG TGGCTCCCCT ACTTTGCCAT CACCACCGAC 
ATCACGGCCT CTGCCATGCG GGTTCACACA GATGGCTCTC TGTGGAGGTA TGTCCGTGCA 
AGCATGTCCT TGTCAGGCTA TATGCCCCCA CTCTGCGACC CCAAGGATGG ACACCTGCTG 
ATGGATGGTG GTTACATCAA CAACCTCCCA GCGGATGTGG CAAGATCCAT GGGGGCAAAG 
GTTGTGATTG CCATCGATGT TGGCAGCCGG GACGAGACAG ACCTCACCAA CTATGGCGAT 
GCCTTGTCTG GATGGTGGCT GCTTTGGAAA CGCTGGAACC CCTTGGCTAC AAAAGTCAAG 
GTGTTGAACA TGGCAGAGAT TCAGACTCGA CTGGCCTATG TGTGCTGCGT CCGGCAGCTG 
GAGATGGTGA AGAACAGCGA CTACTGTGAG TACCTGAGGC CTCCCATCGA CAGTTACCGC 
ACCCTGGACT TCGGCAAATT TGATGAGATC TGTGAAGTTG GGTATCAACA TGGGCGGACA 
GTGTTTGACA TCTGGGTCCG GAGTGGTGTC CTAGAGAAGA TGTTGCAGGA CCAGCAGGGG 
ACAAGCAAGA GGAAGGACTG TGGGGTGTTC ACCTGCCCCA ATTCCTCCTT CACGGACCTT 
GCTGAGATTG TGTCACGGAT TGAGCCGGCC AAGGTGGCTG CAGTGGATGC CTTGCTTGGC 
ACAGCCACTG TCCTTGGAGA GAGGACCAAT GGAGCCCTCT CTTCTGCTCT AGATGAGTCT 
GACTATCAGA CCGAGTATGA GGAGGAACTG CCAGCCATCC CCAAGGAAAC GTATGCTGAC 
TTCCAGAGCA CTGGGATTGA GCTAGACTCT GACTCGGAAT ATGAGCCTTC GATGTTGCAA 
GGACCCCCCA GCTTGACTTC CCCAGAACAG TCCCAGGACT CCTTCCCTTG GCTCCCTAAT 
CAAGATGACC AGGGGCCAAG ACTGGAACAT CCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_011239092.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237394.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu74054
Clone ID Related Accession (Same CDS sequence)	XM_011239092.2 , XM_011239092.3
Accession Version	XM_011239092.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239092.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGTC TGCTGAGTTG TCTGGCTGGC TACTGTTTGG GTACCACCCT GAGCTCCTGG 
AGGCTGCATT TCATGGAGGA GCAGTCCCAG TCCACCATGC TGATGGGGAT TGGAATTGGC 
GCCCTCCTTA CCCTAGCCTT CGTTGGTATT ACCTTCTTTT TTGTATACAG AAGAGTGAGA 
AGATTACGAC GGGCAGAACC CACTCCTCAG TACAGGTTCC GGAAGAGAGA CAAAGTAATG 
TTTTATGGCC GGAAGATCAT GAGGAAGGTG ACCACACTCC CCCATACCTT GGTTGGGAAT 
ACCTCTGCAC CCCGGCAGCG AGTCAGGAAG AGGACCAAAG TGCTGTCTTT AGCCAAGAGG 
ATTCTGCGCT TTAAGAAAGA GTATCCCACT TTGCAGCCAA AAGAGCCTCC GCCCTCCCTG 
CTTGAGGCTG ATCTTACAGA GTTTGATGTG AAGAACTCTC ATCTGCCATC AGAAGTCTTG 
TACATGTTGA AAAATGTTCG GGTTCTAGGC CACTTTGAGA AGCCACTATT TCTGGAACTT 
TGCAAACATA TGGTCTTTGT GCAGCTACAA GAAGGGGAGC ACGTATTCCA ACCTGGGGAG 
CCTGACATCA GCATCTATGT GGTTCAGGAC GGACGGCTGG AGGTCTGCAT CCAAGACGCT 
GTGACACAGG ATGGCACCGA AGTGGTGGTC AAAGAGGTCC TGCCAGGGGA CAGTGTCCAC 
AGTCTCCTCA GCATTTTGGA TGTGATCACC GGTCACACTG CACCATACAA AACAGTTTCT 
GCCCGTGCCG CTGTCTCGTC CACCGTGCTC TGGCTTCCTG CAGCAGCTTT TCAAGGAGTG 
TTTGAGAAAT ACCCTGAAAC TCTGGTCAGG GTTGTGCAGA TCATCATGGT CCGGCTGCAG 
CGGGTGACTT TCCTGGCTCT GCACAACTAC CTGGGGCTGA CTACAGAGCT CTTCAACCCT 
GAGAGCCAGG CCATCCCTCT CTTGTCAGTA GCAAGTGTGG CTGGACGAGC CAAGAGACAG 
ATGTCATATG GTCCAGAGGA GCAGCTTGAG AGGTCCCTAC GGCCCTCGGA GTTCAGCAGC 
TCAGATCATG GGAGCAGCTG TGTGACAGTC TCTGGGCCTC TGCTGAAGAG GAGCTGCTCA 
GTGCCTCTGC CTTCTAACCA TGGGGAGGTG GATGAGCTCA GACAGTCCCA GGGAAGTGGC 
TCTAACACTT CTGCCTTCCA AGAAAGCCAT GAGGGAGCCA CCTCAGACCT GGGGATGGCA 
TACAACCGTG CCAGGATTTT GCCACACTCT GATGAGCAAC TTGGGAACTC TTTGGCCAGC 
AAGTCCAAGA AAAGTGTTGT CGCAGAGACC CCCTCTGCCA TCTTCCACTA CTCAGAAAAT 
TTCAGGGATG AGACTGGGGC CTGTGGGAAG ACAGATGCTA TCTTCAGAGC TGCCACAAAG 
GACCTCCTTA CCCTGATGAA GCTAGATGAC CCATCCCTGT TGGATGGCCG GGTGGCATTT 
CTTCATGTTC CTGCAGGCAC CTTAGTGTCA AAACAGGGAG ATCAGGATGT CAATATTTTG 
TTTGTGGTCT CGGGGATGCT GCATGTGTAC CAGCAAAAGA TTGACAGCTT GGAGGATACT 
TGCCTGTTCC TCACACATCC CGGGGAGATG GTGGGTCAGC TGGCGGTGCT CACTGGAGAA 
CCCCTAATGT TCACCATCAG AGCCAACCGA GACTGCAGCT TTTTGTCCAT CTCGAAGGCC 
CACTTCTATG AGATCATGAG GAAACGGCCG GATGTTGTCC TGGGCGTGGC ACACACAGTT 
GTGAAGAGGA TGTCATCCTT TGTACGGCAG ATTGATTTTG CTCTAGACTG GATGGAAGTA 
GAGGCTGGAC GTGCCATATA CAGGCAAGGA GACAAGTCTG ACTGCACATA CATCGTTCTC 
AGTGGCAGGC TGCGTTCTGT CATCCGAAAA GATGATGGGA AAAAACGCCT GGCAGGGGAA 
TATGGCCGAG GAGACCTTGT TGGTGTGGTG GAGACGCTGA CCCACCAGGC CAGAGCAACC 
ACAGTGCATG CTGTCCGGGA CTCAGAACTG GCTAAGCTGC CAGCAGGAGC TCTGACGTCT 
ATCAAGCGCA GGTACCCACA GGTTGTAACG CGACTGATTC ATCTCTTGGG CGAGAAGATC 
TTAGGCAGTC TCCAGCAGGG ATCTGCAACA GGTCACCAGC TTGGATTTAA CACAGCAAGC 
AGCAAATGGG ACTTGGGGAA CCCGCCTGGC AATCTGTCCA CAGTGGCAGC ACTGCCTGCT 
TCTGAGGATG TGCCCCTAAC CGCCTTTGCT CTGGAGCTCC AACATGCCCT CAGTGCCATC 
GGTCCTGTCC TGCTGCTGAC CAGTGACAAC ATCAAACAAC GCCTGGGTTC CGCTGCTCTA 
GACAGCATCC ATGAGTACCG GCTCTCCAGT TGGCTGGGCC AGCAGGAGGA CATCCACCGG 
ATTGTGCTGT ATCAGGCAGA CGGCACACTC ACACCGTGGA CCCAGCGCTG CATCCGGCAG 
GCTGACTGCA TCCTCATCGT GGGCTTGGGT GAGCAAGAGC CAGCAGTAGG TGAGCTGGAG 
CAGATGTTGG AGAGCACAGC TGTGCGTGCC CAGAAGCAGC TAATCTTGCT GCACAAAGAG 
GACGGTCCAG TGCCTTCCCG CACAGTGGAG TGGCTCAACA TGCGAAGTTG GTGTTCTGGC 
CATTTGCACC TCTGCTGCCC ACGCCGTGTC TTCTCCAAGA GAAGCTTGCC CAAGCTGGTC 
GAGATGTACA CACGTGTTTT CCAACGACCA CCAGACCGTC ATTCTGATTT TTCCCGCCTG 
GCACGGATGC TGACAGGCAA TGCCATTGCC TTGGTGCTTG GAGGGGGTGG AGCTAGAGGG 
TGTGCCCAGG TGGGCATCCT CAGGGCACTA GCAGAGTGTG GCGTCCCCGT GGACATAATC 
GGAGGCACAT CCATCGGGGC TTTCATGGGT GCTCTGTTTG CTGAGGAGCG GAGCTACAGC 
CAAACACGGA TCCGGGCCAA GCAGTGGGCA GAGGGTATGA CATCAATGAT GAAGACTATA 
CTGGACCTGA CCTACCCAAT CACATCCATG TTCTCTGGCA CTGGCTTCAA TAGCAGCATC 
AGCAACATCT TCAAAGACCG GCAGATTGAG GACCTGTGGC TCCCCTACTT TGCCATCACC 
ACCGACATCA CGGCCTCTGC CATGCGGGTT CACACAGATG GCTCTCTGTG GAGGTATGTC 
CGTGCAAGCA TGTCCTTGTC AGGCTATATG CCCCCACTCT GCGACCCCAA GGATGGACAC 
CTGCTGATGG ATGGTGGTTA CATCAACAAC CTCCCAGCGG ATGTGGCAAG ATCCATGGGG 
GCAAAGGTTG TGATTGCCAT CGATGTTGGC AGCCGGGACG AGACAGACCT CACCAACTAT 
GGCGATGCCT TGTCTGGATG GTGGCTGCTT TGGAAACGCT GGAACCCCTT GGCTACAAAA 
GTCAAGGTGT TGAACATGGC AGAGATTCAG ACTCGACTGG CCTATGTGTG CTGCGTCCGG 
CAGCTGGAGA TGGTGAAGAA CAGCGACTAC TGTGAGTACC TGAGGCCTCC CATCGACAGT 
TACCGCACCC TGGACTTCGG CAAATTTGAT GAGATCTGTG AAGTTGGGTA TCAACATGGG 
CGGACAGTGT TTGACATCTG GGTCCGGAGT GGTGTCCTAG AGAAGATGTT GCAGGACCAG 
CAGGGGACAA GCAAGAGGAA GGACTGTGGG GTGTTCACCT GCCCCAATTC CTCCTTCACG 
GACCTTGCTG AGATTGTGTC ACGGATTGAG CCGGCCAAGG TGGCTGCAGT GGATGCCTTG 
CTTGGCACAG CCACTGTCCT TGGAGAGAGG ACCAATGGAG CCCTCTCTTC TGCTCTAGAT 
GAGTCTGACT ATCAGACCGA GTATGAGGAG GAACTGCCAG CCATCCCCAA GGAAACGTAT 
GCTGACTTCC AGAGCACTGG GATTGAGCTA GACTCTGACT CGGAATATGA GCCTTCGATG 
TTGCAAGGAC CCCCCAGCTT GACTTCCCCA GAACAGTCCC AGGACTCCTT CCCTTGGCTC 
CCTAATCAAG ATGACCAGGG GCCAAGACTG GAACATCCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237394.1
CDS	101..4225
Translation	MPGLLSCLAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_011239092.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239092.3

ORF Insert Sequence:

ATGCCTGGTC TGCTGAGTTG TCTGGCTGGC TACTGTTTGG GTACCACCCT GAGCTCCTGG 
AGGCTGCATT TCATGGAGGA GCAGTCCCAG TCCACCATGC TGATGGGGAT TGGAATTGGC 
GCCCTCCTTA CCCTAGCCTT CGTTGGTATT ACCTTCTTTT TTGTATACAG AAGAGTGAGA 
AGATTACGAC GGGCAGAACC CACTCCTCAG TACAGGTTCC GGAAGAGAGA CAAAGTAATG 
TTTTATGGCC GGAAGATCAT GAGGAAGGTG ACCACACTCC CCCATACCTT GGTTGGGAAT 
ACCTCTGCAC CCCGGCAGCG AGTCAGGAAG AGGACCAAAG TGCTGTCTTT AGCCAAGAGG 
ATTCTGCGCT TTAAGAAAGA GTATCCCACT TTGCAGCCAA AAGAGCCTCC GCCCTCCCTG 
CTTGAGGCTG ATCTTACAGA GTTTGATGTG AAGAACTCTC ATCTGCCATC AGAAGTCTTG 
TACATGTTGA AAAATGTTCG GGTTCTAGGC CACTTTGAGA AGCCACTATT TCTGGAACTT 
TGCAAACATA TGGTCTTTGT GCAGCTACAA GAAGGGGAGC ACGTATTCCA ACCTGGGGAG 
CCTGACATCA GCATCTATGT GGTTCAGGAC GGACGGCTGG AGGTCTGCAT CCAAGACGCT 
GTGACACAGG ATGGCACCGA AGTGGTGGTC AAAGAGGTCC TGCCAGGGGA CAGTGTCCAC 
AGTCTCCTCA GCATTTTGGA TGTGATCACC GGTCACACTG CACCATACAA AACAGTTTCT 
GCCCGTGCCG CTGTCTCGTC CACCGTGCTC TGGCTTCCTG CAGCAGCTTT TCAAGGAGTG 
TTTGAGAAAT ACCCTGAAAC TCTGGTCAGG GTTGTGCAGA TCATCATGGT CCGGCTGCAG 
CGGGTGACTT TCCTGGCTCT GCACAACTAC CTGGGGCTGA CTACAGAGCT CTTCAACCCT 
GAGAGCCAGG CCATCCCTCT CTTGTCAGTA GCAAGTGTGG CTGGACGAGC CAAGAGACAG 
ATGTCATATG GTCCAGAGGA GCAGCTTGAG AGGTCCCTAC GGCCCTCGGA GTTCAGCAGC 
TCAGATCATG GGAGCAGCTG TGTGACAGTC TCTGGGCCTC TGCTGAAGAG GAGCTGCTCA 
GTGCCTCTGC CTTCTAACCA TGGGGAGGTG GATGAGCTCA GACAGTCCCA GGGAAGTGGC 
TCTAACACTT CTGCCTTCCA AGAAAGCCAT GAGGGAGCCA CCTCAGACCT GGGGATGGCA 
TACAACCGTG CCAGGATTTT GCCACACTCT GATGAGCAAC TTGGGAACTC TTTGGCCAGC 
AAGTCCAAGA AAAGTGTTGT CGCAGAGACC CCCTCTGCCA TCTTCCACTA CTCAGAAAAT 
TTCAGGGATG AGACTGGGGC CTGTGGGAAG ACAGATGCTA TCTTCAGAGC TGCCACAAAG 
GACCTCCTTA CCCTGATGAA GCTAGATGAC CCATCCCTGT TGGATGGCCG GGTGGCATTT 
CTTCATGTTC CTGCAGGCAC CTTAGTGTCA AAACAGGGAG ATCAGGATGT CAATATTTTG 
TTTGTGGTCT CGGGGATGCT GCATGTGTAC CAGCAAAAGA TTGACAGCTT GGAGGATACT 
TGCCTGTTCC TCACACATCC CGGGGAGATG GTGGGTCAGC TGGCGGTGCT CACTGGAGAA 
CCCCTAATGT TCACCATCAG AGCCAACCGA GACTGCAGCT TTTTGTCCAT CTCGAAGGCC 
CACTTCTATG AGATCATGAG GAAACGGCCG GATGTTGTCC TGGGCGTGGC ACACACAGTT 
GTGAAGAGGA TGTCATCCTT TGTACGGCAG ATTGATTTTG CTCTAGACTG GATGGAAGTA 
GAGGCTGGAC GTGCCATATA CAGGCAAGGA GACAAGTCTG ACTGCACATA CATCGTTCTC 
AGTGGCAGGC TGCGTTCTGT CATCCGAAAA GATGATGGGA AAAAACGCCT GGCAGGGGAA 
TATGGCCGAG GAGACCTTGT TGGTGTGGTG GAGACGCTGA CCCACCAGGC CAGAGCAACC 
ACAGTGCATG CTGTCCGGGA CTCAGAACTG GCTAAGCTGC CAGCAGGAGC TCTGACGTCT 
ATCAAGCGCA GGTACCCACA GGTTGTAACG CGACTGATTC ATCTCTTGGG CGAGAAGATC 
TTAGGCAGTC TCCAGCAGGG ATCTGCAACA GGTCACCAGC TTGGATTTAA CACAGCAAGC 
AGCAAATGGG ACTTGGGGAA CCCGCCTGGC AATCTGTCCA CAGTGGCAGC ACTGCCTGCT 
TCTGAGGATG TGCCCCTAAC CGCCTTTGCT CTGGAGCTCC AACATGCCCT CAGTGCCATC 
GGTCCTGTCC TGCTGCTGAC CAGTGACAAC ATCAAACAAC GCCTGGGTTC CGCTGCTCTA 
GACAGCATCC ATGAGTACCG GCTCTCCAGT TGGCTGGGCC AGCAGGAGGA CATCCACCGG 
ATTGTGCTGT ATCAGGCAGA CGGCACACTC ACACCGTGGA CCCAGCGCTG CATCCGGCAG 
GCTGACTGCA TCCTCATCGT GGGCTTGGGT GAGCAAGAGC CAGCAGTAGG TGAGCTGGAG 
CAGATGTTGG AGAGCACAGC TGTGCGTGCC CAGAAGCAGC TAATCTTGCT GCACAAAGAG 
GACGGTCCAG TGCCTTCCCG CACAGTGGAG TGGCTCAACA TGCGAAGTTG GTGTTCTGGC 
CATTTGCACC TCTGCTGCCC ACGCCGTGTC TTCTCCAAGA GAAGCTTGCC CAAGCTGGTC 
GAGATGTACA CACGTGTTTT CCAACGACCA CCAGACCGTC ATTCTGATTT TTCCCGCCTG 
GCACGGATGC TGACAGGCAA TGCCATTGCC TTGGTGCTTG GAGGGGGTGG AGCTAGAGGG 
TGTGCCCAGG TGGGCATCCT CAGGGCACTA GCAGAGTGTG GCGTCCCCGT GGACATAATC 
GGAGGCACAT CCATCGGGGC TTTCATGGGT GCTCTGTTTG CTGAGGAGCG GAGCTACAGC 
CAAACACGGA TCCGGGCCAA GCAGTGGGCA GAGGGTATGA CATCAATGAT GAAGACTATA 
CTGGACCTGA CCTACCCAAT CACATCCATG TTCTCTGGCA CTGGCTTCAA TAGCAGCATC 
AGCAACATCT TCAAAGACCG GCAGATTGAG GACCTGTGGC TCCCCTACTT TGCCATCACC 
ACCGACATCA CGGCCTCTGC CATGCGGGTT CACACAGATG GCTCTCTGTG GAGGTATGTC 
CGTGCAAGCA TGTCCTTGTC AGGCTATATG CCCCCACTCT GCGACCCCAA GGATGGACAC 
CTGCTGATGG ATGGTGGTTA CATCAACAAC CTCCCAGCGG ATGTGGCAAG ATCCATGGGG 
GCAAAGGTTG TGATTGCCAT CGATGTTGGC AGCCGGGACG AGACAGACCT CACCAACTAT 
GGCGATGCCT TGTCTGGATG GTGGCTGCTT TGGAAACGCT GGAACCCCTT GGCTACAAAA 
GTCAAGGTGT TGAACATGGC AGAGATTCAG ACTCGACTGG CCTATGTGTG CTGCGTCCGG 
CAGCTGGAGA TGGTGAAGAA CAGCGACTAC TGTGAGTACC TGAGGCCTCC CATCGACAGT 
TACCGCACCC TGGACTTCGG CAAATTTGAT GAGATCTGTG AAGTTGGGTA TCAACATGGG 
CGGACAGTGT TTGACATCTG GGTCCGGAGT GGTGTCCTAG AGAAGATGTT GCAGGACCAG 
CAGGGGACAA GCAAGAGGAA GGACTGTGGG GTGTTCACCT GCCCCAATTC CTCCTTCACG 
GACCTTGCTG AGATTGTGTC ACGGATTGAG CCGGCCAAGG TGGCTGCAGT GGATGCCTTG 
CTTGGCACAG CCACTGTCCT TGGAGAGAGG ACCAATGGAG CCCTCTCTTC TGCTCTAGAT 
GAGTCTGACT ATCAGACCGA GTATGAGGAG GAACTGCCAG CCATCCCCAA GGAAACGTAT 
GCTGACTTCC AGAGCACTGG GATTGAGCTA GACTCTGACT CGGAATATGA GCCTTCGATG 
TTGCAAGGAC CCCCCAGCTT GACTTCCCCA GAACAGTCCC AGGACTCCTT CCCTTGGCTC 
CCTAATCAAG ATGACCAGGG GCCAAGACTG GAACATCCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_017318029.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173518.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X11, mRNA.

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CloneID	OMu80246
Clone ID Related Accession (Same CDS sequence)	XM_017318029.2 , XM_017318029.1
Accession Version	XM_017318029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGA TGGCACCGAA 
GTGGTGGTCA AAGAGGTCCT GCCAGGGGAC AGTGTCCACA GTCTCCTCAG CATTTTGGAT 
GTGATCACCG GTCACACTGC ACCATACAAA ACAGTTTCTG CCCGTGCCGC TGTCTCGTCC 
ACCGTGCTCT GGCTTCCTGC AGCAGCTTTT CAAGGAGTGT TTGAGAAATA CCCTGAAACT 
CTGGTCAGGG TTGTGCAGAT CATCATGGTC CGGCTGCAGC GGGTGACTTT CCTGGCTCTG 
CACAACTACC TGGGGCTGAC TACAGAGCTC TTCAACCCTG AGAGCCAGGC CATCCCTCTC 
TTGTCAGTAG CAAGTGTGGC TGGACGAGCC AAGAGACAGA TGTCATATGG TCCAGAGGAG 
CAGCTTGAGA GGTCCCTACG GCCCTCGGAG TTCAGCAGCT CAGATCATGG GAGCAGCTGT 
GTGACAGTCT CTGGGCCTCT GCTGAAGAGG AGCTGCTCAG TGCCTCTGCC TTCTAACCAT 
GGGGAGGTGG ATGAGCTCAG ACAGTCCCAG GGAAGTGGCT CTAACACTTC TGCCTTCCAA 
GAAAGCCATG AGGGAGCCAC CTCAGACCTG GGGATGGCAT ACAACCGTGC CAGGATTTTG 
CCACACTCTG ATGAGCAACT TGGGAACTCT TTGGCCAGCA AGTCCAAGAA AAGTGTTGTC 
GCAGAGACCC CCTCTGCCAT CTTCCACTAC TCAGAAAATT TCAGGGATGA GACTGGGGCC 
TGTGGGAAGA CAGATGCTAT CTTCAGAGCT GCCACAAAGG ACCTCCTTAC CCTGATGAAG 
CTAGATGACC CATCCCTGTT GGATGGCCGG GTGGCATTTC TTCATGTTCC TGCAGGCACC 
TTAGTGTCAA AACAGGGAGA TCAGGATGTC AATATTTTGT TTGTGGTCTC GGGGATGCTG 
CATGTGTACC AGCAAAAGAT TGACAGCTTG GAGGATACTT GCCTGTTCCT CACACATCCC 
GGGGAGATGG TGGGTCAGCT GGCGGTGCTC ACTGGAGAAC CCCTAATGTT CACCATCAGA 
GCCAACCGAG ACTGCAGCTT TTTGTCCATC TCGAAGGCCC ACTTCTATGA GATCATGAGG 
AAACGGCCGG ATGTTGTCCT GGGCGTGGCA CACACAGTTG TGAAGAGGAT GTCATCCTTT 
GTACGGCAGA TTGATTTTGC TCTAGACTGG ATGGAAGTAG AGGCTGGACG TGCCATATAC 
AGGCAAGGAG ACAAGTCTGA CTGCACATAC ATCGTTCTCA GTGGCAGGCT GCGTTCTGTC 
ATCCGAAAAG ATGATGGGAA AAAACGCCTG GCAGGGGAAT ATGGCCGAGG AGACCTTGTT 
GGTGTGGTGG AGACGCTGAC CCACCAGGCC AGAGCAACCA CAGTGCATGC TGTCCGGGAC 
TCAGAACTGG CTAAGCTGCC AGCAGGAGCT CTGACGTCTA TCAAGCGCAG GTACCCACAG 
GTTGTAACGC GACTGATTCA TCTCTTGGGC GAGAAGATCT TAGGCAGTCT CCAGCAGGGA 
TCTGCAACAG GTCACCAGCT TGGATTTAAC ACAGCAAGCA GCAAATGGGA CTTGGGGAAC 
CCGCCTGGCA ATCTGTCCAC AGTGGCAGCA CTGCCTGCTT CTGAGGATGT GCCCCTAACC 
GCCTTTGCTC TGGAGCTCCA ACATGCCCTC AGTGCCATCG GTCCTGTCCT GCTGCTGACC 
AGTGACAACA TCAAACAACG CCTGGGTTCC GCTGCTCTAG ACAGCATCCA TGAGTACCGG 
CTCTCCAGTT GGCTGGGCCA GCAGGAGGAC ATCCACCGGA TTGTGCTGTA TCAGGCAGAC 
GGCACACTCA CACCGTGGAC CCAGCGCTGC ATCCGGCAGG CTGACTGCAT CCTCATCGTG 
GGCTTGGGTG AGCAAGAGCC AGCAGTAGGT GAGCTGGAGC AGATGTTGGA GAGCACAGCT 
GTGCGTGCCC AGAAGCAGCT AATCTTGCTG CACAAAGAGG ACGGTCCAGT GCCTTCCCGC 
ACAGTGGAGT GGCTCAACAT GCGAAGTTGG TGTTCTGGCC ATTTGCACCT CTGCTGCCCA 
CGCCGTGTCT TCTCCAAGAG AAGCTTGCCC AAGCTGGTCG AGATGTACAC ACGTGTTTTC 
CAACGACCAC CAGACCGTCA TTCTGATTTT TCCCGCCTGG CACGGATGCT GACAGGCAAT 
GCCATTGCCT TGGTGCTTGG AGGGGGTGGA GCTAGAGGGT GTGCCCAGGT GGGCATCCTC 
AGGGCACTAG CAGAGTGTGG CGTCCCCGTG GACATAATCG GAGGCACATC CATCGGGGCT 
TTCATGGGTG CTCTGTTTGC TGAGGAGCGG AGCTACAGCC AAACACGGAT CCGGGCCAAG 
CAGTGGGCAG AGGGTATGAC ATCAATGATG AAGACTATAC TGGACCTGAC CTACCCAATC 
ACATCCATGT TCTCTGGCAC TGGCTTCAAT AGCAGCATCA GCAACATCTT CAAAGACCGG 
CAGATTGAGG ACCTGTGGCT CCCCTACTTT GCCATCACCA CCGACATCAC GGCCTCTGCC 
ATGCGGGTTC ACACAGATGG CTCTCTGTGG AGGTATGTCC GTGCAAGCAT GTCCTTGTCA 
GGCTATATGC CCCCACTCTG CGACCCCAAG GATGGACACC TGCTGATGGA TGGTGGTTAC 
ATCAACAACC TCCCAGCGGA TGTGGCAAGA TCCATGGGGG CAAAGGTTGT GATTGCCATC 
GATGTTGGCA GCCGGGACGA GACAGACCTC ACCAACTATG GCGATGCCTT GTCTGGATGG 
TGGCTGCTTT GGAAACGCTG GAACCCCTTG GCTACAAAAG TCAAGGTGTT GAACATGGCA 
GAGATTCAGA CTCGACTGGC CTATGTGTGC TGCGTCCGGC AGCTGGAGAT GGTGAAGAAC 
AGCGACTACT GTGAGTACCT GAGGCCTCCC ATCGACAGTT ACCGCACCCT GGACTTCGGC 
AAATTTGATG AGATCTGTGA AGTTGGGTAT CAACATGGGC GGACAGTGTT TGACATCTGG 
GTCCGGAGTG GTGTCCTAGA GAAGATGTTG CAGGACCAGC AGGGGACAAG CAAGAGGAAG 
GACTGTGGGG TGTTCACCTG CCCCAATTCC TCCTTCACGG ACCTTGCTGA GATTGTGTCA 
CGGATTGAGC CGGCCAAGGT GGCTGCAGTG GATGATGAGT CTGACTATCA GACCGAGTAT 
GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA ACGTATGCTG ACTTCCAGAG CACTGGGATT 
GAGCTAGACT CTGACTCGGA ATATGAGCCT TCGATGTTGC AAGGACCCCC CAGCTTGACT 
TCCCCAGAAC AGTCCCAGGA CTCCTTCCCT TGGCTCCCTA ATCAAGATGA CCAGGGGCCA 
AGACTGGAAC ATCCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173518.1
CDS	469..4449
Translation	MEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDADGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_017318029.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318029.2

ORF Insert Sequence:

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGA TGGCACCGAA 
GTGGTGGTCA AAGAGGTCCT GCCAGGGGAC AGTGTCCACA GTCTCCTCAG CATTTTGGAT 
GTGATCACCG GTCACACTGC ACCATACAAA ACAGTTTCTG CCCGTGCCGC TGTCTCGTCC 
ACCGTGCTCT GGCTTCCTGC AGCAGCTTTT CAAGGAGTGT TTGAGAAATA CCCTGAAACT 
CTGGTCAGGG TTGTGCAGAT CATCATGGTC CGGCTGCAGC GGGTGACTTT CCTGGCTCTG 
CACAACTACC TGGGGCTGAC TACAGAGCTC TTCAACCCTG AGAGCCAGGC CATCCCTCTC 
TTGTCAGTAG CAAGTGTGGC TGGACGAGCC AAGAGACAGA TGTCATATGG TCCAGAGGAG 
CAGCTTGAGA GGTCCCTACG GCCCTCGGAG TTCAGCAGCT CAGATCATGG GAGCAGCTGT 
GTGACAGTCT CTGGGCCTCT GCTGAAGAGG AGCTGCTCAG TGCCTCTGCC TTCTAACCAT 
GGGGAGGTGG ATGAGCTCAG ACAGTCCCAG GGAAGTGGCT CTAACACTTC TGCCTTCCAA 
GAAAGCCATG AGGGAGCCAC CTCAGACCTG GGGATGGCAT ACAACCGTGC CAGGATTTTG 
CCACACTCTG ATGAGCAACT TGGGAACTCT TTGGCCAGCA AGTCCAAGAA AAGTGTTGTC 
GCAGAGACCC CCTCTGCCAT CTTCCACTAC TCAGAAAATT TCAGGGATGA GACTGGGGCC 
TGTGGGAAGA CAGATGCTAT CTTCAGAGCT GCCACAAAGG ACCTCCTTAC CCTGATGAAG 
CTAGATGACC CATCCCTGTT GGATGGCCGG GTGGCATTTC TTCATGTTCC TGCAGGCACC 
TTAGTGTCAA AACAGGGAGA TCAGGATGTC AATATTTTGT TTGTGGTCTC GGGGATGCTG 
CATGTGTACC AGCAAAAGAT TGACAGCTTG GAGGATACTT GCCTGTTCCT CACACATCCC 
GGGGAGATGG TGGGTCAGCT GGCGGTGCTC ACTGGAGAAC CCCTAATGTT CACCATCAGA 
GCCAACCGAG ACTGCAGCTT TTTGTCCATC TCGAAGGCCC ACTTCTATGA GATCATGAGG 
AAACGGCCGG ATGTTGTCCT GGGCGTGGCA CACACAGTTG TGAAGAGGAT GTCATCCTTT 
GTACGGCAGA TTGATTTTGC TCTAGACTGG ATGGAAGTAG AGGCTGGACG TGCCATATAC 
AGGCAAGGAG ACAAGTCTGA CTGCACATAC ATCGTTCTCA GTGGCAGGCT GCGTTCTGTC 
ATCCGAAAAG ATGATGGGAA AAAACGCCTG GCAGGGGAAT ATGGCCGAGG AGACCTTGTT 
GGTGTGGTGG AGACGCTGAC CCACCAGGCC AGAGCAACCA CAGTGCATGC TGTCCGGGAC 
TCAGAACTGG CTAAGCTGCC AGCAGGAGCT CTGACGTCTA TCAAGCGCAG GTACCCACAG 
GTTGTAACGC GACTGATTCA TCTCTTGGGC GAGAAGATCT TAGGCAGTCT CCAGCAGGGA 
TCTGCAACAG GTCACCAGCT TGGATTTAAC ACAGCAAGCA GCAAATGGGA CTTGGGGAAC 
CCGCCTGGCA ATCTGTCCAC AGTGGCAGCA CTGCCTGCTT CTGAGGATGT GCCCCTAACC 
GCCTTTGCTC TGGAGCTCCA ACATGCCCTC AGTGCCATCG GTCCTGTCCT GCTGCTGACC 
AGTGACAACA TCAAACAACG CCTGGGTTCC GCTGCTCTAG ACAGCATCCA TGAGTACCGG 
CTCTCCAGTT GGCTGGGCCA GCAGGAGGAC ATCCACCGGA TTGTGCTGTA TCAGGCAGAC 
GGCACACTCA CACCGTGGAC CCAGCGCTGC ATCCGGCAGG CTGACTGCAT CCTCATCGTG 
GGCTTGGGTG AGCAAGAGCC AGCAGTAGGT GAGCTGGAGC AGATGTTGGA GAGCACAGCT 
GTGCGTGCCC AGAAGCAGCT AATCTTGCTG CACAAAGAGG ACGGTCCAGT GCCTTCCCGC 
ACAGTGGAGT GGCTCAACAT GCGAAGTTGG TGTTCTGGCC ATTTGCACCT CTGCTGCCCA 
CGCCGTGTCT TCTCCAAGAG AAGCTTGCCC AAGCTGGTCG AGATGTACAC ACGTGTTTTC 
CAACGACCAC CAGACCGTCA TTCTGATTTT TCCCGCCTGG CACGGATGCT GACAGGCAAT 
GCCATTGCCT TGGTGCTTGG AGGGGGTGGA GCTAGAGGGT GTGCCCAGGT GGGCATCCTC 
AGGGCACTAG CAGAGTGTGG CGTCCCCGTG GACATAATCG GAGGCACATC CATCGGGGCT 
TTCATGGGTG CTCTGTTTGC TGAGGAGCGG AGCTACAGCC AAACACGGAT CCGGGCCAAG 
CAGTGGGCAG AGGGTATGAC ATCAATGATG AAGACTATAC TGGACCTGAC CTACCCAATC 
ACATCCATGT TCTCTGGCAC TGGCTTCAAT AGCAGCATCA GCAACATCTT CAAAGACCGG 
CAGATTGAGG ACCTGTGGCT CCCCTACTTT GCCATCACCA CCGACATCAC GGCCTCTGCC 
ATGCGGGTTC ACACAGATGG CTCTCTGTGG AGGTATGTCC GTGCAAGCAT GTCCTTGTCA 
GGCTATATGC CCCCACTCTG CGACCCCAAG GATGGACACC TGCTGATGGA TGGTGGTTAC 
ATCAACAACC TCCCAGCGGA TGTGGCAAGA TCCATGGGGG CAAAGGTTGT GATTGCCATC 
GATGTTGGCA GCCGGGACGA GACAGACCTC ACCAACTATG GCGATGCCTT GTCTGGATGG 
TGGCTGCTTT GGAAACGCTG GAACCCCTTG GCTACAAAAG TCAAGGTGTT GAACATGGCA 
GAGATTCAGA CTCGACTGGC CTATGTGTGC TGCGTCCGGC AGCTGGAGAT GGTGAAGAAC 
AGCGACTACT GTGAGTACCT GAGGCCTCCC ATCGACAGTT ACCGCACCCT GGACTTCGGC 
AAATTTGATG AGATCTGTGA AGTTGGGTAT CAACATGGGC GGACAGTGTT TGACATCTGG 
GTCCGGAGTG GTGTCCTAGA GAAGATGTTG CAGGACCAGC AGGGGACAAG CAAGAGGAAG 
GACTGTGGGG TGTTCACCTG CCCCAATTCC TCCTTCACGG ACCTTGCTGA GATTGTGTCA 
CGGATTGAGC CGGCCAAGGT GGCTGCAGTG GATGATGAGT CTGACTATCA GACCGAGTAT 
GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA ACGTATGCTG ACTTCCAGAG CACTGGGATT 
GAGCTAGACT CTGACTCGGA ATATGAGCCT TCGATGTTGC AAGGACCCCC CAGCTTGACT 
TCCCCAGAAC AGTCCCAGGA CTCCTTCCCT TGGCTCCCTA ATCAAGATGA CCAGGGGCCA 
AGACTGGAAC ATCCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_017318029.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173518.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X10, mRNA.

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CloneID	OMu80246
Clone ID Related Accession (Same CDS sequence)	XM_017318029.2 , XM_017318029.1
Accession Version	XM_017318029.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGA TGGCACCGAA 
GTGGTGGTCA AAGAGGTCCT GCCAGGGGAC AGTGTCCACA GTCTCCTCAG CATTTTGGAT 
GTGATCACCG GTCACACTGC ACCATACAAA ACAGTTTCTG CCCGTGCCGC TGTCTCGTCC 
ACCGTGCTCT GGCTTCCTGC AGCAGCTTTT CAAGGAGTGT TTGAGAAATA CCCTGAAACT 
CTGGTCAGGG TTGTGCAGAT CATCATGGTC CGGCTGCAGC GGGTGACTTT CCTGGCTCTG 
CACAACTACC TGGGGCTGAC TACAGAGCTC TTCAACCCTG AGAGCCAGGC CATCCCTCTC 
TTGTCAGTAG CAAGTGTGGC TGGACGAGCC AAGAGACAGA TGTCATATGG TCCAGAGGAG 
CAGCTTGAGA GGTCCCTACG GCCCTCGGAG TTCAGCAGCT CAGATCATGG GAGCAGCTGT 
GTGACAGTCT CTGGGCCTCT GCTGAAGAGG AGCTGCTCAG TGCCTCTGCC TTCTAACCAT 
GGGGAGGTGG ATGAGCTCAG ACAGTCCCAG GGAAGTGGCT CTAACACTTC TGCCTTCCAA 
GAAAGCCATG AGGGAGCCAC CTCAGACCTG GGGATGGCAT ACAACCGTGC CAGGATTTTG 
CCACACTCTG ATGAGCAACT TGGGAACTCT TTGGCCAGCA AGTCCAAGAA AAGTGTTGTC 
GCAGAGACCC CCTCTGCCAT CTTCCACTAC TCAGAAAATT TCAGGGATGA GACTGGGGCC 
TGTGGGAAGA CAGATGCTAT CTTCAGAGCT GCCACAAAGG ACCTCCTTAC CCTGATGAAG 
CTAGATGACC CATCCCTGTT GGATGGCCGG GTGGCATTTC TTCATGTTCC TGCAGGCACC 
TTAGTGTCAA AACAGGGAGA TCAGGATGTC AATATTTTGT TTGTGGTCTC GGGGATGCTG 
CATGTGTACC AGCAAAAGAT TGACAGCTTG GAGGATACTT GCCTGTTCCT CACACATCCC 
GGGGAGATGG TGGGTCAGCT GGCGGTGCTC ACTGGAGAAC CCCTAATGTT CACCATCAGA 
GCCAACCGAG ACTGCAGCTT TTTGTCCATC TCGAAGGCCC ACTTCTATGA GATCATGAGG 
AAACGGCCGG ATGTTGTCCT GGGCGTGGCA CACACAGTTG TGAAGAGGAT GTCATCCTTT 
GTACGGCAGA TTGATTTTGC TCTAGACTGG ATGGAAGTAG AGGCTGGACG TGCCATATAC 
AGGCAAGGAG ACAAGTCTGA CTGCACATAC ATCGTTCTCA GTGGCAGGCT GCGTTCTGTC 
ATCCGAAAAG ATGATGGGAA AAAACGCCTG GCAGGGGAAT ATGGCCGAGG AGACCTTGTT 
GGTGTGGTGG AGACGCTGAC CCACCAGGCC AGAGCAACCA CAGTGCATGC TGTCCGGGAC 
TCAGAACTGG CTAAGCTGCC AGCAGGAGCT CTGACGTCTA TCAAGCGCAG GTACCCACAG 
GTTGTAACGC GACTGATTCA TCTCTTGGGC GAGAAGATCT TAGGCAGTCT CCAGCAGGGA 
TCTGCAACAG GTCACCAGCT TGGATTTAAC ACAGCAAGCA GCAAATGGGA CTTGGGGAAC 
CCGCCTGGCA ATCTGTCCAC AGTGGCAGCA CTGCCTGCTT CTGAGGATGT GCCCCTAACC 
GCCTTTGCTC TGGAGCTCCA ACATGCCCTC AGTGCCATCG GTCCTGTCCT GCTGCTGACC 
AGTGACAACA TCAAACAACG CCTGGGTTCC GCTGCTCTAG ACAGCATCCA TGAGTACCGG 
CTCTCCAGTT GGCTGGGCCA GCAGGAGGAC ATCCACCGGA TTGTGCTGTA TCAGGCAGAC 
GGCACACTCA CACCGTGGAC CCAGCGCTGC ATCCGGCAGG CTGACTGCAT CCTCATCGTG 
GGCTTGGGTG AGCAAGAGCC AGCAGTAGGT GAGCTGGAGC AGATGTTGGA GAGCACAGCT 
GTGCGTGCCC AGAAGCAGCT AATCTTGCTG CACAAAGAGG ACGGTCCAGT GCCTTCCCGC 
ACAGTGGAGT GGCTCAACAT GCGAAGTTGG TGTTCTGGCC ATTTGCACCT CTGCTGCCCA 
CGCCGTGTCT TCTCCAAGAG AAGCTTGCCC AAGCTGGTCG AGATGTACAC ACGTGTTTTC 
CAACGACCAC CAGACCGTCA TTCTGATTTT TCCCGCCTGG CACGGATGCT GACAGGCAAT 
GCCATTGCCT TGGTGCTTGG AGGGGGTGGA GCTAGAGGGT GTGCCCAGGT GGGCATCCTC 
AGGGCACTAG CAGAGTGTGG CGTCCCCGTG GACATAATCG GAGGCACATC CATCGGGGCT 
TTCATGGGTG CTCTGTTTGC TGAGGAGCGG AGCTACAGCC AAACACGGAT CCGGGCCAAG 
CAGTGGGCAG AGGGTATGAC ATCAATGATG AAGACTATAC TGGACCTGAC CTACCCAATC 
ACATCCATGT TCTCTGGCAC TGGCTTCAAT AGCAGCATCA GCAACATCTT CAAAGACCGG 
CAGATTGAGG ACCTGTGGCT CCCCTACTTT GCCATCACCA CCGACATCAC GGCCTCTGCC 
ATGCGGGTTC ACACAGATGG CTCTCTGTGG AGGTATGTCC GTGCAAGCAT GTCCTTGTCA 
GGCTATATGC CCCCACTCTG CGACCCCAAG GATGGACACC TGCTGATGGA TGGTGGTTAC 
ATCAACAACC TCCCAGCGGA TGTGGCAAGA TCCATGGGGG CAAAGGTTGT GATTGCCATC 
GATGTTGGCA GCCGGGACGA GACAGACCTC ACCAACTATG GCGATGCCTT GTCTGGATGG 
TGGCTGCTTT GGAAACGCTG GAACCCCTTG GCTACAAAAG TCAAGGTGTT GAACATGGCA 
GAGATTCAGA CTCGACTGGC CTATGTGTGC TGCGTCCGGC AGCTGGAGAT GGTGAAGAAC 
AGCGACTACT GTGAGTACCT GAGGCCTCCC ATCGACAGTT ACCGCACCCT GGACTTCGGC 
AAATTTGATG AGATCTGTGA AGTTGGGTAT CAACATGGGC GGACAGTGTT TGACATCTGG 
GTCCGGAGTG GTGTCCTAGA GAAGATGTTG CAGGACCAGC AGGGGACAAG CAAGAGGAAG 
GACTGTGGGG TGTTCACCTG CCCCAATTCC TCCTTCACGG ACCTTGCTGA GATTGTGTCA 
CGGATTGAGC CGGCCAAGGT GGCTGCAGTG GATGATGAGT CTGACTATCA GACCGAGTAT 
GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA ACGTATGCTG ACTTCCAGAG CACTGGGATT 
GAGCTAGACT CTGACTCGGA ATATGAGCCT TCGATGTTGC AAGGACCCCC CAGCTTGACT 
TCCCCAGAAC AGTCCCAGGA CTCCTTCCCT TGGCTCCCTA ATCAAGATGA CCAGGGGCCA 
AGACTGGAAC ATCCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173518.1
CDS	1150..5130
Translation	MEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDADGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_017318029.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318029.1

ORF Insert Sequence:

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGA TGGCACCGAA 
GTGGTGGTCA AAGAGGTCCT GCCAGGGGAC AGTGTCCACA GTCTCCTCAG CATTTTGGAT 
GTGATCACCG GTCACACTGC ACCATACAAA ACAGTTTCTG CCCGTGCCGC TGTCTCGTCC 
ACCGTGCTCT GGCTTCCTGC AGCAGCTTTT CAAGGAGTGT TTGAGAAATA CCCTGAAACT 
CTGGTCAGGG TTGTGCAGAT CATCATGGTC CGGCTGCAGC GGGTGACTTT CCTGGCTCTG 
CACAACTACC TGGGGCTGAC TACAGAGCTC TTCAACCCTG AGAGCCAGGC CATCCCTCTC 
TTGTCAGTAG CAAGTGTGGC TGGACGAGCC AAGAGACAGA TGTCATATGG TCCAGAGGAG 
CAGCTTGAGA GGTCCCTACG GCCCTCGGAG TTCAGCAGCT CAGATCATGG GAGCAGCTGT 
GTGACAGTCT CTGGGCCTCT GCTGAAGAGG AGCTGCTCAG TGCCTCTGCC TTCTAACCAT 
GGGGAGGTGG ATGAGCTCAG ACAGTCCCAG GGAAGTGGCT CTAACACTTC TGCCTTCCAA 
GAAAGCCATG AGGGAGCCAC CTCAGACCTG GGGATGGCAT ACAACCGTGC CAGGATTTTG 
CCACACTCTG ATGAGCAACT TGGGAACTCT TTGGCCAGCA AGTCCAAGAA AAGTGTTGTC 
GCAGAGACCC CCTCTGCCAT CTTCCACTAC TCAGAAAATT TCAGGGATGA GACTGGGGCC 
TGTGGGAAGA CAGATGCTAT CTTCAGAGCT GCCACAAAGG ACCTCCTTAC CCTGATGAAG 
CTAGATGACC CATCCCTGTT GGATGGCCGG GTGGCATTTC TTCATGTTCC TGCAGGCACC 
TTAGTGTCAA AACAGGGAGA TCAGGATGTC AATATTTTGT TTGTGGTCTC GGGGATGCTG 
CATGTGTACC AGCAAAAGAT TGACAGCTTG GAGGATACTT GCCTGTTCCT CACACATCCC 
GGGGAGATGG TGGGTCAGCT GGCGGTGCTC ACTGGAGAAC CCCTAATGTT CACCATCAGA 
GCCAACCGAG ACTGCAGCTT TTTGTCCATC TCGAAGGCCC ACTTCTATGA GATCATGAGG 
AAACGGCCGG ATGTTGTCCT GGGCGTGGCA CACACAGTTG TGAAGAGGAT GTCATCCTTT 
GTACGGCAGA TTGATTTTGC TCTAGACTGG ATGGAAGTAG AGGCTGGACG TGCCATATAC 
AGGCAAGGAG ACAAGTCTGA CTGCACATAC ATCGTTCTCA GTGGCAGGCT GCGTTCTGTC 
ATCCGAAAAG ATGATGGGAA AAAACGCCTG GCAGGGGAAT ATGGCCGAGG AGACCTTGTT 
GGTGTGGTGG AGACGCTGAC CCACCAGGCC AGAGCAACCA CAGTGCATGC TGTCCGGGAC 
TCAGAACTGG CTAAGCTGCC AGCAGGAGCT CTGACGTCTA TCAAGCGCAG GTACCCACAG 
GTTGTAACGC GACTGATTCA TCTCTTGGGC GAGAAGATCT TAGGCAGTCT CCAGCAGGGA 
TCTGCAACAG GTCACCAGCT TGGATTTAAC ACAGCAAGCA GCAAATGGGA CTTGGGGAAC 
CCGCCTGGCA ATCTGTCCAC AGTGGCAGCA CTGCCTGCTT CTGAGGATGT GCCCCTAACC 
GCCTTTGCTC TGGAGCTCCA ACATGCCCTC AGTGCCATCG GTCCTGTCCT GCTGCTGACC 
AGTGACAACA TCAAACAACG CCTGGGTTCC GCTGCTCTAG ACAGCATCCA TGAGTACCGG 
CTCTCCAGTT GGCTGGGCCA GCAGGAGGAC ATCCACCGGA TTGTGCTGTA TCAGGCAGAC 
GGCACACTCA CACCGTGGAC CCAGCGCTGC ATCCGGCAGG CTGACTGCAT CCTCATCGTG 
GGCTTGGGTG AGCAAGAGCC AGCAGTAGGT GAGCTGGAGC AGATGTTGGA GAGCACAGCT 
GTGCGTGCCC AGAAGCAGCT AATCTTGCTG CACAAAGAGG ACGGTCCAGT GCCTTCCCGC 
ACAGTGGAGT GGCTCAACAT GCGAAGTTGG TGTTCTGGCC ATTTGCACCT CTGCTGCCCA 
CGCCGTGTCT TCTCCAAGAG AAGCTTGCCC AAGCTGGTCG AGATGTACAC ACGTGTTTTC 
CAACGACCAC CAGACCGTCA TTCTGATTTT TCCCGCCTGG CACGGATGCT GACAGGCAAT 
GCCATTGCCT TGGTGCTTGG AGGGGGTGGA GCTAGAGGGT GTGCCCAGGT GGGCATCCTC 
AGGGCACTAG CAGAGTGTGG CGTCCCCGTG GACATAATCG GAGGCACATC CATCGGGGCT 
TTCATGGGTG CTCTGTTTGC TGAGGAGCGG AGCTACAGCC AAACACGGAT CCGGGCCAAG 
CAGTGGGCAG AGGGTATGAC ATCAATGATG AAGACTATAC TGGACCTGAC CTACCCAATC 
ACATCCATGT TCTCTGGCAC TGGCTTCAAT AGCAGCATCA GCAACATCTT CAAAGACCGG 
CAGATTGAGG ACCTGTGGCT CCCCTACTTT GCCATCACCA CCGACATCAC GGCCTCTGCC 
ATGCGGGTTC ACACAGATGG CTCTCTGTGG AGGTATGTCC GTGCAAGCAT GTCCTTGTCA 
GGCTATATGC CCCCACTCTG CGACCCCAAG GATGGACACC TGCTGATGGA TGGTGGTTAC 
ATCAACAACC TCCCAGCGGA TGTGGCAAGA TCCATGGGGG CAAAGGTTGT GATTGCCATC 
GATGTTGGCA GCCGGGACGA GACAGACCTC ACCAACTATG GCGATGCCTT GTCTGGATGG 
TGGCTGCTTT GGAAACGCTG GAACCCCTTG GCTACAAAAG TCAAGGTGTT GAACATGGCA 
GAGATTCAGA CTCGACTGGC CTATGTGTGC TGCGTCCGGC AGCTGGAGAT GGTGAAGAAC 
AGCGACTACT GTGAGTACCT GAGGCCTCCC ATCGACAGTT ACCGCACCCT GGACTTCGGC 
AAATTTGATG AGATCTGTGA AGTTGGGTAT CAACATGGGC GGACAGTGTT TGACATCTGG 
GTCCGGAGTG GTGTCCTAGA GAAGATGTTG CAGGACCAGC AGGGGACAAG CAAGAGGAAG 
GACTGTGGGG TGTTCACCTG CCCCAATTCC TCCTTCACGG ACCTTGCTGA GATTGTGTCA 
CGGATTGAGC CGGCCAAGGT GGCTGCAGTG GATGATGAGT CTGACTATCA GACCGAGTAT 
GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA ACGTATGCTG ACTTCCAGAG CACTGGGATT 
GAGCTAGACT CTGACTCGGA ATATGAGCCT TCGATGTTGC AAGGACCCCC CAGCTTGACT 
TCCCCAGAAC AGTCCCAGGA CTCCTTCCCT TGGCTCCCTA ATCAAGATGA CCAGGGGCCA 
AGACTGGAAC ATCCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_017318030.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173519.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu80247
Clone ID Related Accession (Same CDS sequence)	XM_017318030.2 , XM_017318030.1
Accession Version	XM_017318030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGATG GCACCGAAGT GGTGGTCAAA GAGGTCCTGC CAGGGGACAG TGTCCACAGT 
CTCCTCAGCA TTTTGGATGT GATCACCGGT CACACTGCAC CATACAAAAC AGTTTCTGCC 
CGTGCCGCTG TCTCGTCCAC CGTGCTCTGG CTTCCTGCAG CAGCTTTTCA AGGAGTGTTT 
GAGAAATACC CTGAAACTCT GGTCAGGGTT GTGCAGATCA TCATGGTCCG GCTGCAGCGG 
GTGACTTTCC TGGCTCTGCA CAACTACCTG GGGCTGACTA CAGAGCTCTT CAACCCTGAG 
AGCCAGGCCA TCCCTCTCTT GTCAGTAGCA AGTGTGGCTG GACGAGCCAA GAGACAGATG 
TCATATGGTC CAGAGGAGCA GCTTGAGAGG TCCCTACGGC CCTCGGAGTT CAGCAGCTCA 
GATCATGGGA GCAGCTGTGT GACAGTCTCT GGGCCTCTGC TGAAGAGGAG CTGCTCAGTG 
CCTCTGCCTT CTAACCATGG GGAGGTGGAT GAGCTCAGAC AGTCCCAGGG AAGTGGCTCT 
AACACTTCTG CCTTCCAAGA AAGCCATGAG GGAGCCACCT CAGACCTGGG GATGGCATAC 
AACCGTGCCA GGATTTTGCC ACACTCTGAT GAGCAACTTG GGAACTCTTT GGCCAGCAAG 
TCCAAGAAAA GTGTTGTCGC AGAGACCCCC TCTGCCATCT TCCACTACTC AGAAAATTTC 
AGGGATGAGA CTGGGGCCTG TGGGAAGACA GATGCTATCT TCAGAGCTGC CACAAAGGAC 
CTCCTTACCC TGATGAAGCT AGATGACCCA TCCCTGTTGG ATGGCCGGGT GGCATTTCTT 
CATGTTCCTG CAGGCACCTT AGTGTCAAAA CAGGGAGATC AGGATGTCAA TATTTTGTTT 
GTGGTCTCGG GGATGCTGCA TGTGTACCAG CAAAAGATTG ACAGCTTGGA GGATACTTGC 
CTGTTCCTCA CACATCCCGG GGAGATGGTG GGTCAGCTGG CGGTGCTCAC TGGAGAACCC 
CTAATGTTCA CCATCAGAGC CAACCGAGAC TGCAGCTTTT TGTCCATCTC GAAGGCCCAC 
TTCTATGAGA TCATGAGGAA ACGGCCGGAT GTTGTCCTGG GCGTGGCACA CACAGTTGTG 
AAGAGGATGT CATCCTTTGT ACGGCAGATT GATTTTGCTC TAGACTGGAT GGAAGTAGAG 
GCTGGACGTG CCATATACAG GCAAGGAGAC AAGTCTGACT GCACATACAT CGTTCTCAGT 
GGCAGGCTGC GTTCTGTCAT CCGAAAAGAT GATGGGAAAA AACGCCTGGC AGGGGAATAT 
GGCCGAGGAG ACCTTGTTGG TGTGGTGGAG ACGCTGACCC ACCAGGCCAG AGCAACCACA 
GTGCATGCTG TCCGGGACTC AGAACTGGCT AAGCTGCCAG CAGGAGCTCT GACGTCTATC 
AAGCGCAGGT ACCCACAGGT TGTAACGCGA CTGATTCATC TCTTGGGCGA GAAGATCTTA 
GGCAGTCTCC AGCAGGGATC TGCAACAGGT CACCAGCTTG GATTTAACAC AGCAAGCAGC 
AAATGGGACT TGGGGAACCC GCCTGGCAAT CTGTCCACAG TGGCAGCACT GCCTGCTTCT 
GAGGATGTGC CCCTAACCGC CTTTGCTCTG GAGCTCCAAC ATGCCCTCAG TGCCATCGGT 
CCTGTCCTGC TGCTGACCAG TGACAACATC AAACAACGCC TGGGTTCCGC TGCTCTAGAC 
AGCATCCATG AGTACCGGCT CTCCAGTTGG CTGGGCCAGC AGGAGGACAT CCACCGGATT 
GTGCTGTATC AGGCAGACGG CACACTCACA CCGTGGACCC AGCGCTGCAT CCGGCAGGCT 
GACTGCATCC TCATCGTGGG CTTGGGTGAG CAAGAGCCAG CAGTAGGTGA GCTGGAGCAG 
ATGTTGGAGA GCACAGCTGT GCGTGCCCAG AAGCAGCTAA TCTTGCTGCA CAAAGAGGAC 
GGTCCAGTGC CTTCCCGCAC AGTGGAGTGG CTCAACATGC GAAGTTGGTG TTCTGGCCAT 
TTGCACCTCT GCTGCCCACG CCGTGTCTTC TCCAAGAGAA GCTTGCCCAA GCTGGTCGAG 
ATGTACACAC GTGTTTTCCA ACGACCACCA GACCGTCATT CTGATTTTTC CCGCCTGGCA 
CGGATGCTGA CAGGCAATGC CATTGCCTTG GTGCTTGGAG GGGGTGGAGC TAGAGGGTGT 
GCCCAGGTGG GCATCCTCAG GGCACTAGCA GAGTGTGGCG TCCCCGTGGA CATAATCGGA 
GGCACATCCA TCGGGGCTTT CATGGGTGCT CTGTTTGCTG AGGAGCGGAG CTACAGCCAA 
ACACGGATCC GGGCCAAGCA GTGGGCAGAG GGTATGACAT CAATGATGAA GACTATACTG 
GACCTGACCT ACCCAATCAC ATCCATGTTC TCTGGCACTG GCTTCAATAG CAGCATCAGC 
AACATCTTCA AAGACCGGCA GATTGAGGAC CTGTGGCTCC CCTACTTTGC CATCACCACC 
GACATCACGG CCTCTGCCAT GCGGGTTCAC ACAGATGGCT CTCTGTGGAG GTATGTCCGT 
GCAAGCATGT CCTTGTCAGG CTATATGCCC CCACTCTGCG ACCCCAAGGA TGGACACCTG 
CTGATGGATG GTGGTTACAT CAACAACCTC CCAGCGGATG TGGCAAGATC CATGGGGGCA 
AAGGTTGTGA TTGCCATCGA TGTTGGCAGC CGGGACGAGA CAGACCTCAC CAACTATGGC 
GATGCCTTGT CTGGATGGTG GCTGCTTTGG AAACGCTGGA ACCCCTTGGC TACAAAAGTC 
AAGGTGTTGA ACATGGCAGA GATTCAGACT CGACTGGCCT ATGTGTGCTG CGTCCGGCAG 
CTGGAGATGG TGAAGAACAG CGACTACTGT GAGTACCTGA GGCCTCCCAT CGACAGTTAC 
CGCACCCTGG ACTTCGGCAA ATTTGATGAG ATCTGTGAAG TTGGGTATCA ACATGGGCGG 
ACAGTGTTTG ACATCTGGGT CCGGAGTGGT GTCCTAGAGA AGATGTTGCA GGACCAGCAG 
GGGACAAGCA AGAGGAAGGA CTGTGGGGTG TTCACCTGCC CCAATTCCTC CTTCACGGAC 
CTTGCTGAGA TTGTGTCACG GATTGAGCCG GCCAAGGTGG CTGCAGTGGA TGATGAGTCT 
GACTATCAGA CCGAGTATGA GGAGGAACTG CCAGCCATCC CCAAGGAAAC GTATGCTGAC 
TTCCAGAGCA CTGGGATTGA GCTAGACTCT GACTCGGTGA GGAATATGAG CCTTCGATGT 
TGCAAGGACC CCCCAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173519.1
CDS	295..4275
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDADGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSVRNMSLRCCKDPPA

Target ORF information:

RefSeq Version	XM_017318030.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318030.2

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGATG GCACCGAAGT GGTGGTCAAA GAGGTCCTGC CAGGGGACAG TGTCCACAGT 
CTCCTCAGCA TTTTGGATGT GATCACCGGT CACACTGCAC CATACAAAAC AGTTTCTGCC 
CGTGCCGCTG TCTCGTCCAC CGTGCTCTGG CTTCCTGCAG CAGCTTTTCA AGGAGTGTTT 
GAGAAATACC CTGAAACTCT GGTCAGGGTT GTGCAGATCA TCATGGTCCG GCTGCAGCGG 
GTGACTTTCC TGGCTCTGCA CAACTACCTG GGGCTGACTA CAGAGCTCTT CAACCCTGAG 
AGCCAGGCCA TCCCTCTCTT GTCAGTAGCA AGTGTGGCTG GACGAGCCAA GAGACAGATG 
TCATATGGTC CAGAGGAGCA GCTTGAGAGG TCCCTACGGC CCTCGGAGTT CAGCAGCTCA 
GATCATGGGA GCAGCTGTGT GACAGTCTCT GGGCCTCTGC TGAAGAGGAG CTGCTCAGTG 
CCTCTGCCTT CTAACCATGG GGAGGTGGAT GAGCTCAGAC AGTCCCAGGG AAGTGGCTCT 
AACACTTCTG CCTTCCAAGA AAGCCATGAG GGAGCCACCT CAGACCTGGG GATGGCATAC 
AACCGTGCCA GGATTTTGCC ACACTCTGAT GAGCAACTTG GGAACTCTTT GGCCAGCAAG 
TCCAAGAAAA GTGTTGTCGC AGAGACCCCC TCTGCCATCT TCCACTACTC AGAAAATTTC 
AGGGATGAGA CTGGGGCCTG TGGGAAGACA GATGCTATCT TCAGAGCTGC CACAAAGGAC 
CTCCTTACCC TGATGAAGCT AGATGACCCA TCCCTGTTGG ATGGCCGGGT GGCATTTCTT 
CATGTTCCTG CAGGCACCTT AGTGTCAAAA CAGGGAGATC AGGATGTCAA TATTTTGTTT 
GTGGTCTCGG GGATGCTGCA TGTGTACCAG CAAAAGATTG ACAGCTTGGA GGATACTTGC 
CTGTTCCTCA CACATCCCGG GGAGATGGTG GGTCAGCTGG CGGTGCTCAC TGGAGAACCC 
CTAATGTTCA CCATCAGAGC CAACCGAGAC TGCAGCTTTT TGTCCATCTC GAAGGCCCAC 
TTCTATGAGA TCATGAGGAA ACGGCCGGAT GTTGTCCTGG GCGTGGCACA CACAGTTGTG 
AAGAGGATGT CATCCTTTGT ACGGCAGATT GATTTTGCTC TAGACTGGAT GGAAGTAGAG 
GCTGGACGTG CCATATACAG GCAAGGAGAC AAGTCTGACT GCACATACAT CGTTCTCAGT 
GGCAGGCTGC GTTCTGTCAT CCGAAAAGAT GATGGGAAAA AACGCCTGGC AGGGGAATAT 
GGCCGAGGAG ACCTTGTTGG TGTGGTGGAG ACGCTGACCC ACCAGGCCAG AGCAACCACA 
GTGCATGCTG TCCGGGACTC AGAACTGGCT AAGCTGCCAG CAGGAGCTCT GACGTCTATC 
AAGCGCAGGT ACCCACAGGT TGTAACGCGA CTGATTCATC TCTTGGGCGA GAAGATCTTA 
GGCAGTCTCC AGCAGGGATC TGCAACAGGT CACCAGCTTG GATTTAACAC AGCAAGCAGC 
AAATGGGACT TGGGGAACCC GCCTGGCAAT CTGTCCACAG TGGCAGCACT GCCTGCTTCT 
GAGGATGTGC CCCTAACCGC CTTTGCTCTG GAGCTCCAAC ATGCCCTCAG TGCCATCGGT 
CCTGTCCTGC TGCTGACCAG TGACAACATC AAACAACGCC TGGGTTCCGC TGCTCTAGAC 
AGCATCCATG AGTACCGGCT CTCCAGTTGG CTGGGCCAGC AGGAGGACAT CCACCGGATT 
GTGCTGTATC AGGCAGACGG CACACTCACA CCGTGGACCC AGCGCTGCAT CCGGCAGGCT 
GACTGCATCC TCATCGTGGG CTTGGGTGAG CAAGAGCCAG CAGTAGGTGA GCTGGAGCAG 
ATGTTGGAGA GCACAGCTGT GCGTGCCCAG AAGCAGCTAA TCTTGCTGCA CAAAGAGGAC 
GGTCCAGTGC CTTCCCGCAC AGTGGAGTGG CTCAACATGC GAAGTTGGTG TTCTGGCCAT 
TTGCACCTCT GCTGCCCACG CCGTGTCTTC TCCAAGAGAA GCTTGCCCAA GCTGGTCGAG 
ATGTACACAC GTGTTTTCCA ACGACCACCA GACCGTCATT CTGATTTTTC CCGCCTGGCA 
CGGATGCTGA CAGGCAATGC CATTGCCTTG GTGCTTGGAG GGGGTGGAGC TAGAGGGTGT 
GCCCAGGTGG GCATCCTCAG GGCACTAGCA GAGTGTGGCG TCCCCGTGGA CATAATCGGA 
GGCACATCCA TCGGGGCTTT CATGGGTGCT CTGTTTGCTG AGGAGCGGAG CTACAGCCAA 
ACACGGATCC GGGCCAAGCA GTGGGCAGAG GGTATGACAT CAATGATGAA GACTATACTG 
GACCTGACCT ACCCAATCAC ATCCATGTTC TCTGGCACTG GCTTCAATAG CAGCATCAGC 
AACATCTTCA AAGACCGGCA GATTGAGGAC CTGTGGCTCC CCTACTTTGC CATCACCACC 
GACATCACGG CCTCTGCCAT GCGGGTTCAC ACAGATGGCT CTCTGTGGAG GTATGTCCGT 
GCAAGCATGT CCTTGTCAGG CTATATGCCC CCACTCTGCG ACCCCAAGGA TGGACACCTG 
CTGATGGATG GTGGTTACAT CAACAACCTC CCAGCGGATG TGGCAAGATC CATGGGGGCA 
AAGGTTGTGA TTGCCATCGA TGTTGGCAGC CGGGACGAGA CAGACCTCAC CAACTATGGC 
GATGCCTTGT CTGGATGGTG GCTGCTTTGG AAACGCTGGA ACCCCTTGGC TACAAAAGTC 
AAGGTGTTGA ACATGGCAGA GATTCAGACT CGACTGGCCT ATGTGTGCTG CGTCCGGCAG 
CTGGAGATGG TGAAGAACAG CGACTACTGT GAGTACCTGA GGCCTCCCAT CGACAGTTAC 
CGCACCCTGG ACTTCGGCAA ATTTGATGAG ATCTGTGAAG TTGGGTATCA ACATGGGCGG 
ACAGTGTTTG ACATCTGGGT CCGGAGTGGT GTCCTAGAGA AGATGTTGCA GGACCAGCAG 
GGGACAAGCA AGAGGAAGGA CTGTGGGGTG TTCACCTGCC CCAATTCCTC CTTCACGGAC 
CTTGCTGAGA TTGTGTCACG GATTGAGCCG GCCAAGGTGG CTGCAGTGGA TGATGAGTCT 
GACTATCAGA CCGAGTATGA GGAGGAACTG CCAGCCATCC CCAAGGAAAC GTATGCTGAC 
TTCCAGAGCA CTGGGATTGA GCTAGACTCT GACTCGGTGA GGAATATGAG CCTTCGATGT 
TGCAAGGACC CCCCAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_017318030.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173519.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X11, mRNA.

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CloneID	OMu80247
Clone ID Related Accession (Same CDS sequence)	XM_017318030.2 , XM_017318030.1
Accession Version	XM_017318030.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGATG GCACCGAAGT GGTGGTCAAA GAGGTCCTGC CAGGGGACAG TGTCCACAGT 
CTCCTCAGCA TTTTGGATGT GATCACCGGT CACACTGCAC CATACAAAAC AGTTTCTGCC 
CGTGCCGCTG TCTCGTCCAC CGTGCTCTGG CTTCCTGCAG CAGCTTTTCA AGGAGTGTTT 
GAGAAATACC CTGAAACTCT GGTCAGGGTT GTGCAGATCA TCATGGTCCG GCTGCAGCGG 
GTGACTTTCC TGGCTCTGCA CAACTACCTG GGGCTGACTA CAGAGCTCTT CAACCCTGAG 
AGCCAGGCCA TCCCTCTCTT GTCAGTAGCA AGTGTGGCTG GACGAGCCAA GAGACAGATG 
TCATATGGTC CAGAGGAGCA GCTTGAGAGG TCCCTACGGC CCTCGGAGTT CAGCAGCTCA 
GATCATGGGA GCAGCTGTGT GACAGTCTCT GGGCCTCTGC TGAAGAGGAG CTGCTCAGTG 
CCTCTGCCTT CTAACCATGG GGAGGTGGAT GAGCTCAGAC AGTCCCAGGG AAGTGGCTCT 
AACACTTCTG CCTTCCAAGA AAGCCATGAG GGAGCCACCT CAGACCTGGG GATGGCATAC 
AACCGTGCCA GGATTTTGCC ACACTCTGAT GAGCAACTTG GGAACTCTTT GGCCAGCAAG 
TCCAAGAAAA GTGTTGTCGC AGAGACCCCC TCTGCCATCT TCCACTACTC AGAAAATTTC 
AGGGATGAGA CTGGGGCCTG TGGGAAGACA GATGCTATCT TCAGAGCTGC CACAAAGGAC 
CTCCTTACCC TGATGAAGCT AGATGACCCA TCCCTGTTGG ATGGCCGGGT GGCATTTCTT 
CATGTTCCTG CAGGCACCTT AGTGTCAAAA CAGGGAGATC AGGATGTCAA TATTTTGTTT 
GTGGTCTCGG GGATGCTGCA TGTGTACCAG CAAAAGATTG ACAGCTTGGA GGATACTTGC 
CTGTTCCTCA CACATCCCGG GGAGATGGTG GGTCAGCTGG CGGTGCTCAC TGGAGAACCC 
CTAATGTTCA CCATCAGAGC CAACCGAGAC TGCAGCTTTT TGTCCATCTC GAAGGCCCAC 
TTCTATGAGA TCATGAGGAA ACGGCCGGAT GTTGTCCTGG GCGTGGCACA CACAGTTGTG 
AAGAGGATGT CATCCTTTGT ACGGCAGATT GATTTTGCTC TAGACTGGAT GGAAGTAGAG 
GCTGGACGTG CCATATACAG GCAAGGAGAC AAGTCTGACT GCACATACAT CGTTCTCAGT 
GGCAGGCTGC GTTCTGTCAT CCGAAAAGAT GATGGGAAAA AACGCCTGGC AGGGGAATAT 
GGCCGAGGAG ACCTTGTTGG TGTGGTGGAG ACGCTGACCC ACCAGGCCAG AGCAACCACA 
GTGCATGCTG TCCGGGACTC AGAACTGGCT AAGCTGCCAG CAGGAGCTCT GACGTCTATC 
AAGCGCAGGT ACCCACAGGT TGTAACGCGA CTGATTCATC TCTTGGGCGA GAAGATCTTA 
GGCAGTCTCC AGCAGGGATC TGCAACAGGT CACCAGCTTG GATTTAACAC AGCAAGCAGC 
AAATGGGACT TGGGGAACCC GCCTGGCAAT CTGTCCACAG TGGCAGCACT GCCTGCTTCT 
GAGGATGTGC CCCTAACCGC CTTTGCTCTG GAGCTCCAAC ATGCCCTCAG TGCCATCGGT 
CCTGTCCTGC TGCTGACCAG TGACAACATC AAACAACGCC TGGGTTCCGC TGCTCTAGAC 
AGCATCCATG AGTACCGGCT CTCCAGTTGG CTGGGCCAGC AGGAGGACAT CCACCGGATT 
GTGCTGTATC AGGCAGACGG CACACTCACA CCGTGGACCC AGCGCTGCAT CCGGCAGGCT 
GACTGCATCC TCATCGTGGG CTTGGGTGAG CAAGAGCCAG CAGTAGGTGA GCTGGAGCAG 
ATGTTGGAGA GCACAGCTGT GCGTGCCCAG AAGCAGCTAA TCTTGCTGCA CAAAGAGGAC 
GGTCCAGTGC CTTCCCGCAC AGTGGAGTGG CTCAACATGC GAAGTTGGTG TTCTGGCCAT 
TTGCACCTCT GCTGCCCACG CCGTGTCTTC TCCAAGAGAA GCTTGCCCAA GCTGGTCGAG 
ATGTACACAC GTGTTTTCCA ACGACCACCA GACCGTCATT CTGATTTTTC CCGCCTGGCA 
CGGATGCTGA CAGGCAATGC CATTGCCTTG GTGCTTGGAG GGGGTGGAGC TAGAGGGTGT 
GCCCAGGTGG GCATCCTCAG GGCACTAGCA GAGTGTGGCG TCCCCGTGGA CATAATCGGA 
GGCACATCCA TCGGGGCTTT CATGGGTGCT CTGTTTGCTG AGGAGCGGAG CTACAGCCAA 
ACACGGATCC GGGCCAAGCA GTGGGCAGAG GGTATGACAT CAATGATGAA GACTATACTG 
GACCTGACCT ACCCAATCAC ATCCATGTTC TCTGGCACTG GCTTCAATAG CAGCATCAGC 
AACATCTTCA AAGACCGGCA GATTGAGGAC CTGTGGCTCC CCTACTTTGC CATCACCACC 
GACATCACGG CCTCTGCCAT GCGGGTTCAC ACAGATGGCT CTCTGTGGAG GTATGTCCGT 
GCAAGCATGT CCTTGTCAGG CTATATGCCC CCACTCTGCG ACCCCAAGGA TGGACACCTG 
CTGATGGATG GTGGTTACAT CAACAACCTC CCAGCGGATG TGGCAAGATC CATGGGGGCA 
AAGGTTGTGA TTGCCATCGA TGTTGGCAGC CGGGACGAGA CAGACCTCAC CAACTATGGC 
GATGCCTTGT CTGGATGGTG GCTGCTTTGG AAACGCTGGA ACCCCTTGGC TACAAAAGTC 
AAGGTGTTGA ACATGGCAGA GATTCAGACT CGACTGGCCT ATGTGTGCTG CGTCCGGCAG 
CTGGAGATGG TGAAGAACAG CGACTACTGT GAGTACCTGA GGCCTCCCAT CGACAGTTAC 
CGCACCCTGG ACTTCGGCAA ATTTGATGAG ATCTGTGAAG TTGGGTATCA ACATGGGCGG 
ACAGTGTTTG ACATCTGGGT CCGGAGTGGT GTCCTAGAGA AGATGTTGCA GGACCAGCAG 
GGGACAAGCA AGAGGAAGGA CTGTGGGGTG TTCACCTGCC CCAATTCCTC CTTCACGGAC 
CTTGCTGAGA TTGTGTCACG GATTGAGCCG GCCAAGGTGG CTGCAGTGGA TGATGAGTCT 
GACTATCAGA CCGAGTATGA GGAGGAACTG CCAGCCATCC CCAAGGAAAC GTATGCTGAC 
TTCCAGAGCA CTGGGATTGA GCTAGACTCT GACTCGGTGA GGAATATGAG CCTTCGATGT 
TGCAAGGACC CCCCAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173519.1
CDS	976..4956
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDADGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSVRNMSLRCCKDPPA

Target ORF information:

RefSeq Version	XM_017318030.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318030.1

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGATG GCACCGAAGT GGTGGTCAAA GAGGTCCTGC CAGGGGACAG TGTCCACAGT 
CTCCTCAGCA TTTTGGATGT GATCACCGGT CACACTGCAC CATACAAAAC AGTTTCTGCC 
CGTGCCGCTG TCTCGTCCAC CGTGCTCTGG CTTCCTGCAG CAGCTTTTCA AGGAGTGTTT 
GAGAAATACC CTGAAACTCT GGTCAGGGTT GTGCAGATCA TCATGGTCCG GCTGCAGCGG 
GTGACTTTCC TGGCTCTGCA CAACTACCTG GGGCTGACTA CAGAGCTCTT CAACCCTGAG 
AGCCAGGCCA TCCCTCTCTT GTCAGTAGCA AGTGTGGCTG GACGAGCCAA GAGACAGATG 
TCATATGGTC CAGAGGAGCA GCTTGAGAGG TCCCTACGGC CCTCGGAGTT CAGCAGCTCA 
GATCATGGGA GCAGCTGTGT GACAGTCTCT GGGCCTCTGC TGAAGAGGAG CTGCTCAGTG 
CCTCTGCCTT CTAACCATGG GGAGGTGGAT GAGCTCAGAC AGTCCCAGGG AAGTGGCTCT 
AACACTTCTG CCTTCCAAGA AAGCCATGAG GGAGCCACCT CAGACCTGGG GATGGCATAC 
AACCGTGCCA GGATTTTGCC ACACTCTGAT GAGCAACTTG GGAACTCTTT GGCCAGCAAG 
TCCAAGAAAA GTGTTGTCGC AGAGACCCCC TCTGCCATCT TCCACTACTC AGAAAATTTC 
AGGGATGAGA CTGGGGCCTG TGGGAAGACA GATGCTATCT TCAGAGCTGC CACAAAGGAC 
CTCCTTACCC TGATGAAGCT AGATGACCCA TCCCTGTTGG ATGGCCGGGT GGCATTTCTT 
CATGTTCCTG CAGGCACCTT AGTGTCAAAA CAGGGAGATC AGGATGTCAA TATTTTGTTT 
GTGGTCTCGG GGATGCTGCA TGTGTACCAG CAAAAGATTG ACAGCTTGGA GGATACTTGC 
CTGTTCCTCA CACATCCCGG GGAGATGGTG GGTCAGCTGG CGGTGCTCAC TGGAGAACCC 
CTAATGTTCA CCATCAGAGC CAACCGAGAC TGCAGCTTTT TGTCCATCTC GAAGGCCCAC 
TTCTATGAGA TCATGAGGAA ACGGCCGGAT GTTGTCCTGG GCGTGGCACA CACAGTTGTG 
AAGAGGATGT CATCCTTTGT ACGGCAGATT GATTTTGCTC TAGACTGGAT GGAAGTAGAG 
GCTGGACGTG CCATATACAG GCAAGGAGAC AAGTCTGACT GCACATACAT CGTTCTCAGT 
GGCAGGCTGC GTTCTGTCAT CCGAAAAGAT GATGGGAAAA AACGCCTGGC AGGGGAATAT 
GGCCGAGGAG ACCTTGTTGG TGTGGTGGAG ACGCTGACCC ACCAGGCCAG AGCAACCACA 
GTGCATGCTG TCCGGGACTC AGAACTGGCT AAGCTGCCAG CAGGAGCTCT GACGTCTATC 
AAGCGCAGGT ACCCACAGGT TGTAACGCGA CTGATTCATC TCTTGGGCGA GAAGATCTTA 
GGCAGTCTCC AGCAGGGATC TGCAACAGGT CACCAGCTTG GATTTAACAC AGCAAGCAGC 
AAATGGGACT TGGGGAACCC GCCTGGCAAT CTGTCCACAG TGGCAGCACT GCCTGCTTCT 
GAGGATGTGC CCCTAACCGC CTTTGCTCTG GAGCTCCAAC ATGCCCTCAG TGCCATCGGT 
CCTGTCCTGC TGCTGACCAG TGACAACATC AAACAACGCC TGGGTTCCGC TGCTCTAGAC 
AGCATCCATG AGTACCGGCT CTCCAGTTGG CTGGGCCAGC AGGAGGACAT CCACCGGATT 
GTGCTGTATC AGGCAGACGG CACACTCACA CCGTGGACCC AGCGCTGCAT CCGGCAGGCT 
GACTGCATCC TCATCGTGGG CTTGGGTGAG CAAGAGCCAG CAGTAGGTGA GCTGGAGCAG 
ATGTTGGAGA GCACAGCTGT GCGTGCCCAG AAGCAGCTAA TCTTGCTGCA CAAAGAGGAC 
GGTCCAGTGC CTTCCCGCAC AGTGGAGTGG CTCAACATGC GAAGTTGGTG TTCTGGCCAT 
TTGCACCTCT GCTGCCCACG CCGTGTCTTC TCCAAGAGAA GCTTGCCCAA GCTGGTCGAG 
ATGTACACAC GTGTTTTCCA ACGACCACCA GACCGTCATT CTGATTTTTC CCGCCTGGCA 
CGGATGCTGA CAGGCAATGC CATTGCCTTG GTGCTTGGAG GGGGTGGAGC TAGAGGGTGT 
GCCCAGGTGG GCATCCTCAG GGCACTAGCA GAGTGTGGCG TCCCCGTGGA CATAATCGGA 
GGCACATCCA TCGGGGCTTT CATGGGTGCT CTGTTTGCTG AGGAGCGGAG CTACAGCCAA 
ACACGGATCC GGGCCAAGCA GTGGGCAGAG GGTATGACAT CAATGATGAA GACTATACTG 
GACCTGACCT ACCCAATCAC ATCCATGTTC TCTGGCACTG GCTTCAATAG CAGCATCAGC 
AACATCTTCA AAGACCGGCA GATTGAGGAC CTGTGGCTCC CCTACTTTGC CATCACCACC 
GACATCACGG CCTCTGCCAT GCGGGTTCAC ACAGATGGCT CTCTGTGGAG GTATGTCCGT 
GCAAGCATGT CCTTGTCAGG CTATATGCCC CCACTCTGCG ACCCCAAGGA TGGACACCTG 
CTGATGGATG GTGGTTACAT CAACAACCTC CCAGCGGATG TGGCAAGATC CATGGGGGCA 
AAGGTTGTGA TTGCCATCGA TGTTGGCAGC CGGGACGAGA CAGACCTCAC CAACTATGGC 
GATGCCTTGT CTGGATGGTG GCTGCTTTGG AAACGCTGGA ACCCCTTGGC TACAAAAGTC 
AAGGTGTTGA ACATGGCAGA GATTCAGACT CGACTGGCCT ATGTGTGCTG CGTCCGGCAG 
CTGGAGATGG TGAAGAACAG CGACTACTGT GAGTACCTGA GGCCTCCCAT CGACAGTTAC 
CGCACCCTGG ACTTCGGCAA ATTTGATGAG ATCTGTGAAG TTGGGTATCA ACATGGGCGG 
ACAGTGTTTG ACATCTGGGT CCGGAGTGGT GTCCTAGAGA AGATGTTGCA GGACCAGCAG 
GGGACAAGCA AGAGGAAGGA CTGTGGGGTG TTCACCTGCC CCAATTCCTC CTTCACGGAC 
CTTGCTGAGA TTGTGTCACG GATTGAGCCG GCCAAGGTGG CTGCAGTGGA TGATGAGTCT 
GACTATCAGA CCGAGTATGA GGAGGAACTG CCAGCCATCC CCAAGGAAAC GTATGCTGAC 
TTCCAGAGCA CTGGGATTGA GCTAGACTCT GACTCGGTGA GGAATATGAG CCTTCGATGT 
TGCAAGGACC CCCCAGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_030250966.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106826.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X9, mRNA.

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CloneID	OMu87865
Clone ID Related Accession (Same CDS sequence)	XM_030250966.1
Accession Version	XM_030250966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGA TGGCACCGAA 
GTGGTGGTCA AAGAGGTCCT GCCAGGGGAC AGTGTCCACA GTCTCCTCAG CATTTTGGAT 
GTGATCACCG GTCACACTGC ACCATACAAA ACAGTTTCTG CCCGTGCCGC TGTCTCGTCC 
ACCGTGCTCT GGCTTCCTGC AGCAGCTTTT CAAGGAGTGT TTGAGAAATA CCCTGAAACT 
CTGGTCAGGG TTGTGCAGAT CATCATGGTC CGGCTGCAGC GGGTGACTTT CCTGGCTCTG 
CACAACTACC TGGGGCTGAC TACAGAGCTC TTCAACCCTG AGAGCCAGGC CATCCCTCTC 
TTGTCAGTAG CAAGTGTGGC TGGACGAGCC AAGAGACAGA TGTCATATGG TCCAGAGGAG 
CAGCTTGAGA GGTCCCTACG GCCCTCGGAG TTCAGCAGCT CAGATCATGG GAGCAGCTGT 
GTGACAGTCT CTGGGCCTCT GCTGAAGAGG AGCTGCTCAG TGCCTCTGCC TTCTAACCAT 
GGGGAGGTGG ATGAGCTCAG ACAGTCCCAG GGAAGTGGCT CTAACACTTC TGCCTTCCAA 
GAAAGCCATG AGGGAGCCAC CTCAGACCTG GGGATGGCAT ACAACCGTGC CAGGATTTTG 
CCACACTCTG ATGAGCAACT TGGGAACTCT TTGGCCAGCA AGTCCAAGAA AAGTGTTGTC 
GCAGAGACCC CCTCTGCCAT CTTCCACTAC TCAGAAAATT TCAGGGATGA GACTGGGGCC 
TGTGGGAAGA CAGATGCTAT CTTCAGAGCT GCCACAAAGG ACCTCCTTAC CCTGATGAAG 
CTAGATGACC CATCCCTGTT GGATGGCCGG GTGGCATTTC TTCATGTTCC TGCAGGCACC 
TTAGTGTCAA AACAGGGAGA TCAGGATGTC AATATTTTGT TTGTGGTCTC GGGGATGCTG 
CATGTGTACC AGCAAAAGAT TGACAGCTTG GAGGATACTT GCCTGTTCCT CACACATCCC 
GGGGAGATGG TGGGTCAGCT GGCGGTGCTC ACTGGAGAAC CCCTAATGTT CACCATCAGA 
GCCAACCGAG ACTGCAGCTT TTTGTCCATC TCGAAGGCCC ACTTCTATGA GATCATGAGG 
AAACGGCCGG ATGTTGTCCT GGGCGTGGCA CACACAGTTG TGAAGAGGAT GTCATCCTTT 
GTACGGCAGA TTGATTTTGC TCTAGACTGG ATGGAAGTAG AGGCTGGACG TGCCATATAC 
AGGCAAGGAG ACAAGTCTGA CTGCACATAC ATCGTTCTCA GTGGCAGGCT GCGTTCTGTC 
ATCCGAAAAG ATGATGGGAA AAAACGCCTG GCAGGGGAAT ATGGCCGAGG AGACCTTGTT 
GGTGTGGTGG AGACGCTGAC CCACCAGGCC AGAGCAACCA CAGTGCATGC TGTCCGGGAC 
TCAGAACTGG CTAAGCTGCC AGCAGGAGCT CTGACGTCTA TCAAGCGCAG GTACCCACAG 
GTTGTAACGC GACTGATTCA TCTCTTGGGC GAGAAGATCT TAGGCAGTCT CCAGCAGGGA 
TCTGCAACAG GTCACCAGCT TGGATTTAAC ACAGCAAGCA GCAAATGGGA CTTGGGGAAC 
CCGCCTGGCA ATCTGTCCAC AGTGGCAGCA CTGCCTGCTT CTGAGGATGT GCCCCTAACC 
GCCTTTGCTC TGGAGCTCCA ACATGCCCTC AGTGCCATCG GTCCTGTCCT GCTGCTGACC 
AGTGACAACA TCAAACAACG CCTGGGTTCC GCTGCTCTAG ACAGCATCCA TGAGTACCGG 
CTCTCCAGTT GGCTGGGCCA GCAGGAGGAC ATCCACCGGA TTGTGCTGTA TCAGGCAGAC 
GGCACACTCA CACCGTGGAC CCAGCGCTGC ATCCGGCAGG CTGACTGCAT CCTCATCGTG 
GGCTTGGGTG AGCAAGAGCC AGCAGTAGGT GAGCTGGAGC AGATGTTGGA GAGCACAGCT 
GTGCGTGCCC AGAAGCAGCT AATCTTGCTG CACAAAGAGG ACGGTCCAGT GCCTTCCCGC 
ACAGTGGAGT GGCTCAACAT GCGAAGTTGG TGTTCTGGCC ATTTGCACCT CTGCTGCCCA 
CGCCGTGTCT TCTCCAAGAG AAGCTTGCCC AAGCTGGTCG AGATGTACAC ACGTGTTTTC 
CAACGACCAC CAGACCGTCA TTCTGATTTT TCCCGCCTGG CACGGATGCT GACAGGCAAT 
GCCATTGCCT TGGTGCTTGG AGGGGGTGGA GCTAGAGGGT GTGCCCAGGT GGGCATCCTC 
AGGGCACTAG CAGAGTGTGG CGTCCCCGTG GACATAATCG GAGGCACATC CATCGGGGCT 
TTCATGGGTG CTCTGTTTGC TGAGGAGCGG AGCTACAGCC AAACACGGAT CCGGGCCAAG 
CAGTGGGCAG AGGGTATGAC ATCAATGATG AAGACTATAC TGGACCTGAC CTACCCAATC 
ACATCCATGT TCTCTGGCAC TGGCTTCAAT AGCAGCATCA GCAACATCTT CAAAGACCGG 
CAGATTGAGG ACCTGTGGCT CCCCTACTTT GCCATCACCA CCGACATCAC GGCCTCTGCC 
ATGCGGGTTC ACACAGATGG CTCTCTGTGG AGGTATGTCC GTGCAAGCAT GTCCTTGTCA 
GGCTATATGC CCCCACTCTG CGACCCCAAG GATGGACACC TGCTGATGGA TGGTGGTTAC 
ATCAACAACC TCCCAGCGGA TGTGGCAAGA TCCATGGGGG CAAAGGTTGT GATTGCCATC 
GATGTTGGCA GCCGGGACGA GACAGACCTC ACCAACTATG GCGATGCCTT GTCTGGATGG 
TGGCTGCTTT GGAAACGCTG GAACCCCTTG GCTACAAAAG TCAAGGTGTT GAACATGGCA 
GAGATTCAGA CTCGACTGGC CTATGTGTGC TGCGTCCGGC AGCTGGAGAT GGTGAAGAAC 
AGCGACTACT GTGAGTACCT GAGGCCTCCC ATCGACAGTT ACCGCACCCT GGACTTCGGC 
AAATTTGATG AGATCTGTGA AGTTGGGTAT CAACATGGGC GGACAGTGTT TGACATCTGG 
GTCCGGAGTG GTGTCCTAGA GAAGATGTTG CAGGACCAGC AGGGGACAAG CAAGAGGAAG 
GACTGTGGGG TGTTCACCTG CCCCAATTCC TCCTTCACGG ACCTTGCTGA GATTGTGTCA 
CGGATTGAGC CGGCCAAGGT GGCTGCAGTG GATGCCTTGC TTGGCACAGC CACTGTCCTT 
GGAGAGAGGA CCAATGGAGC CCTCTCTTCT GCTCTAGATG AGTCTGACTA TCAGACCGAG 
TATGAGGAGG AACTGCCAGC CATCCCCAAG GAAACGTATG CTGACTTCCA GAGCACTGGG 
ATTGAGCTAG ACTCTGACTC GGAATATGAG CCTTCGATGT TGCAAGGACC CCCCAGCTTG 
ACTTCCCCAG AACAGTCCCA GGACTCCTTC CCTTGGCTCC CTAATCAAGA TGACCAGGGG 
CCAAGACTGG AACATCCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106826.1
CDS	469..4512
Translation	MEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDADGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_030250966.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250966.1

ORF Insert Sequence:

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGA TGGCACCGAA 
GTGGTGGTCA AAGAGGTCCT GCCAGGGGAC AGTGTCCACA GTCTCCTCAG CATTTTGGAT 
GTGATCACCG GTCACACTGC ACCATACAAA ACAGTTTCTG CCCGTGCCGC TGTCTCGTCC 
ACCGTGCTCT GGCTTCCTGC AGCAGCTTTT CAAGGAGTGT TTGAGAAATA CCCTGAAACT 
CTGGTCAGGG TTGTGCAGAT CATCATGGTC CGGCTGCAGC GGGTGACTTT CCTGGCTCTG 
CACAACTACC TGGGGCTGAC TACAGAGCTC TTCAACCCTG AGAGCCAGGC CATCCCTCTC 
TTGTCAGTAG CAAGTGTGGC TGGACGAGCC AAGAGACAGA TGTCATATGG TCCAGAGGAG 
CAGCTTGAGA GGTCCCTACG GCCCTCGGAG TTCAGCAGCT CAGATCATGG GAGCAGCTGT 
GTGACAGTCT CTGGGCCTCT GCTGAAGAGG AGCTGCTCAG TGCCTCTGCC TTCTAACCAT 
GGGGAGGTGG ATGAGCTCAG ACAGTCCCAG GGAAGTGGCT CTAACACTTC TGCCTTCCAA 
GAAAGCCATG AGGGAGCCAC CTCAGACCTG GGGATGGCAT ACAACCGTGC CAGGATTTTG 
CCACACTCTG ATGAGCAACT TGGGAACTCT TTGGCCAGCA AGTCCAAGAA AAGTGTTGTC 
GCAGAGACCC CCTCTGCCAT CTTCCACTAC TCAGAAAATT TCAGGGATGA GACTGGGGCC 
TGTGGGAAGA CAGATGCTAT CTTCAGAGCT GCCACAAAGG ACCTCCTTAC CCTGATGAAG 
CTAGATGACC CATCCCTGTT GGATGGCCGG GTGGCATTTC TTCATGTTCC TGCAGGCACC 
TTAGTGTCAA AACAGGGAGA TCAGGATGTC AATATTTTGT TTGTGGTCTC GGGGATGCTG 
CATGTGTACC AGCAAAAGAT TGACAGCTTG GAGGATACTT GCCTGTTCCT CACACATCCC 
GGGGAGATGG TGGGTCAGCT GGCGGTGCTC ACTGGAGAAC CCCTAATGTT CACCATCAGA 
GCCAACCGAG ACTGCAGCTT TTTGTCCATC TCGAAGGCCC ACTTCTATGA GATCATGAGG 
AAACGGCCGG ATGTTGTCCT GGGCGTGGCA CACACAGTTG TGAAGAGGAT GTCATCCTTT 
GTACGGCAGA TTGATTTTGC TCTAGACTGG ATGGAAGTAG AGGCTGGACG TGCCATATAC 
AGGCAAGGAG ACAAGTCTGA CTGCACATAC ATCGTTCTCA GTGGCAGGCT GCGTTCTGTC 
ATCCGAAAAG ATGATGGGAA AAAACGCCTG GCAGGGGAAT ATGGCCGAGG AGACCTTGTT 
GGTGTGGTGG AGACGCTGAC CCACCAGGCC AGAGCAACCA CAGTGCATGC TGTCCGGGAC 
TCAGAACTGG CTAAGCTGCC AGCAGGAGCT CTGACGTCTA TCAAGCGCAG GTACCCACAG 
GTTGTAACGC GACTGATTCA TCTCTTGGGC GAGAAGATCT TAGGCAGTCT CCAGCAGGGA 
TCTGCAACAG GTCACCAGCT TGGATTTAAC ACAGCAAGCA GCAAATGGGA CTTGGGGAAC 
CCGCCTGGCA ATCTGTCCAC AGTGGCAGCA CTGCCTGCTT CTGAGGATGT GCCCCTAACC 
GCCTTTGCTC TGGAGCTCCA ACATGCCCTC AGTGCCATCG GTCCTGTCCT GCTGCTGACC 
AGTGACAACA TCAAACAACG CCTGGGTTCC GCTGCTCTAG ACAGCATCCA TGAGTACCGG 
CTCTCCAGTT GGCTGGGCCA GCAGGAGGAC ATCCACCGGA TTGTGCTGTA TCAGGCAGAC 
GGCACACTCA CACCGTGGAC CCAGCGCTGC ATCCGGCAGG CTGACTGCAT CCTCATCGTG 
GGCTTGGGTG AGCAAGAGCC AGCAGTAGGT GAGCTGGAGC AGATGTTGGA GAGCACAGCT 
GTGCGTGCCC AGAAGCAGCT AATCTTGCTG CACAAAGAGG ACGGTCCAGT GCCTTCCCGC 
ACAGTGGAGT GGCTCAACAT GCGAAGTTGG TGTTCTGGCC ATTTGCACCT CTGCTGCCCA 
CGCCGTGTCT TCTCCAAGAG AAGCTTGCCC AAGCTGGTCG AGATGTACAC ACGTGTTTTC 
CAACGACCAC CAGACCGTCA TTCTGATTTT TCCCGCCTGG CACGGATGCT GACAGGCAAT 
GCCATTGCCT TGGTGCTTGG AGGGGGTGGA GCTAGAGGGT GTGCCCAGGT GGGCATCCTC 
AGGGCACTAG CAGAGTGTGG CGTCCCCGTG GACATAATCG GAGGCACATC CATCGGGGCT 
TTCATGGGTG CTCTGTTTGC TGAGGAGCGG AGCTACAGCC AAACACGGAT CCGGGCCAAG 
CAGTGGGCAG AGGGTATGAC ATCAATGATG AAGACTATAC TGGACCTGAC CTACCCAATC 
ACATCCATGT TCTCTGGCAC TGGCTTCAAT AGCAGCATCA GCAACATCTT CAAAGACCGG 
CAGATTGAGG ACCTGTGGCT CCCCTACTTT GCCATCACCA CCGACATCAC GGCCTCTGCC 
ATGCGGGTTC ACACAGATGG CTCTCTGTGG AGGTATGTCC GTGCAAGCAT GTCCTTGTCA 
GGCTATATGC CCCCACTCTG CGACCCCAAG GATGGACACC TGCTGATGGA TGGTGGTTAC 
ATCAACAACC TCCCAGCGGA TGTGGCAAGA TCCATGGGGG CAAAGGTTGT GATTGCCATC 
GATGTTGGCA GCCGGGACGA GACAGACCTC ACCAACTATG GCGATGCCTT GTCTGGATGG 
TGGCTGCTTT GGAAACGCTG GAACCCCTTG GCTACAAAAG TCAAGGTGTT GAACATGGCA 
GAGATTCAGA CTCGACTGGC CTATGTGTGC TGCGTCCGGC AGCTGGAGAT GGTGAAGAAC 
AGCGACTACT GTGAGTACCT GAGGCCTCCC ATCGACAGTT ACCGCACCCT GGACTTCGGC 
AAATTTGATG AGATCTGTGA AGTTGGGTAT CAACATGGGC GGACAGTGTT TGACATCTGG 
GTCCGGAGTG GTGTCCTAGA GAAGATGTTG CAGGACCAGC AGGGGACAAG CAAGAGGAAG 
GACTGTGGGG TGTTCACCTG CCCCAATTCC TCCTTCACGG ACCTTGCTGA GATTGTGTCA 
CGGATTGAGC CGGCCAAGGT GGCTGCAGTG GATGCCTTGC TTGGCACAGC CACTGTCCTT 
GGAGAGAGGA CCAATGGAGC CCTCTCTTCT GCTCTAGATG AGTCTGACTA TCAGACCGAG 
TATGAGGAGG AACTGCCAGC CATCCCCAAG GAAACGTATG CTGACTTCCA GAGCACTGGG 
ATTGAGCTAG ACTCTGACTC GGAATATGAG CCTTCGATGT TGCAAGGACC CCCCAGCTTG 
ACTTCCCCAG AACAGTCCCA GGACTCCTTC CCTTGGCTCC CTAATCAAGA TGACCAGGGG 
CCAAGACTGG AACATCCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498021.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498084.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu29542
Clone ID Related Accession (Same CDS sequence)	XM_006498021.3 , XM_006498021.4
Accession Version	XM_006498021.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498021.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGTGAG GAATATGAGC 
CTTCGATGTT GCAAGGACCC CCCAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498084.1
CDS	977..4966
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSVRNMSLRCCKDPPA

Target ORF information:

RefSeq Version	XM_006498021.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498021.3

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGTGAG GAATATGAGC 
CTTCGATGTT GCAAGGACCC CCCAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498022.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498085.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X12, mRNA.

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CloneID	OMu29543
Clone ID Related Accession (Same CDS sequence)	XM_006498022.3 , XM_006498022.2
Accession Version	XM_006498022.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTATG GCCGGAAGAT CATGAGGAAG GTGACCACAC TCCCCCATAC CTTGGTTGGG 
AATACCTCTG CACCCCGGCA GCGAGTCAGG AAGAGGACCA AAGTGCTGTC TTTAGCCAAG 
AGGATTCTGC GCTTTAAGAA AGAGTATCCC ACTTTGCAGC CAAAAGAGCC TCCGCCCTCC 
CTGCTTGAGG CTGATCTTAC AGAGTTTGAT GTGAAGAACT CTCATCTGCC ATCAGAAGTC 
TTGTACATGT TGAAAAATGT TCGGGTTCTA GGCCACTTTG AGAAGCCACT ATTTCTGGAA 
CTTTGCAAAC ATATGGTCTT TGTGCAGCTA CAAGAAGGGG AGCACGTATT CCAACCTGGG 
GAGCCTGACA TCAGCATCTA TGTGGTTCAG GACGGACGGC TGGAGGTCTG CATCCAAGAC 
GCTGTGACAC AGGATGGCAC CGAAGTGGTG GTCAAAGAGG TCCTGCCAGG GGACAGTGTC 
CACAGTCTCC TCAGCATTTT GGATGTGATC ACCGGTCACA CTGCACCATA CAAAACAGTT 
TCTGCCCGTG CCGCTGTCTC GTCCACCGTG CTCTGGCTTC CTGCAGCAGC TTTTCAAGGA 
GTGTTTGAGA AATACCCTGA AACTCTGGTC AGGGTTGTGC AGATCATCAT GGTCCGGCTG 
CAGCGGGTGA CTTTCCTGGC TCTGCACAAC TACCTGGGGC TGACTACAGA GCTCTTCAAC 
CCTGAGAGCC AGGCCATCCC TCTCTTGTCA GTAGCAAGTG TGGCTGGACG AGCCAAGAGA 
CAGATGTCAT ATGGTCCAGA GGAGCAGCTT GAGAGGTCCC TACGGCCCTC GGAGTTCAGC 
AGCTCAGATC ATGGGAGCAG CTGTGTGACA GTCTCTGGGC CTCTGCTGAA GAGGAGCTGC 
TCAGTGCCTC TGCCTTCTAA CCATGGGGAG GTGGATGAGC TCAGACAGTC CCAGGGAAGT 
GGCTCTAACA CTTCTGCCTT CCAAGAAAGC CATGAGGGAG CCACCTCAGA CCTGGGGATG 
GCATACAACC GTGCCAGGAT TTTGCCACAC TCTGATGAGC AACTTGGGAA CTCTTTGGCC 
AGCAAGTCCA AGAAAAGTGT TGTCGCAGAG ACCCCCTCTG CCATCTTCCA CTACTCAGAA 
AATTTCAGGG ATGAGACTGG GGCCTGTGGG AAGACAGATG CTATCTTCAG AGCTGCCACA 
AAGGACCTCC TTACCCTGAT GAAGCTAGAT GACCCATCCC TGTTGGATGG CCGGGTGGCA 
TTTCTTCATG TTCCTGCAGG CACCTTAGTG TCAAAACAGG GAGATCAGGA TGTCAATATT 
TTGTTTGTGG TCTCGGGGAT GCTGCATGTG TACCAGCAAA AGATTGACAG CTTGGAGGAT 
ACTTGCCTGT TCCTCACACA TCCCGGGGAG ATGGTGGGTC AGCTGGCGGT GCTCACTGGA 
GAACCCCTAA TGTTCACCAT CAGAGCCAAC CGAGACTGCA GCTTTTTGTC CATCTCGAAG 
GCCCACTTCT ATGAGATCAT GAGGAAACGG CCGGATGTTG TCCTGGGCGT GGCACACACA 
GTTGTGAAGA GGATGTCATC CTTTGTACGG CAGATTGATT TTGCTCTAGA CTGGATGGAA 
GTAGAGGCTG GACGTGCCAT ATACAGGCAA GGAGACAAGT CTGACTGCAC ATACATCGTT 
CTCAGTGGCA GGCTGCGTTC TGTCATCCGA AAAGATGATG GGAAAAAACG CCTGGCAGGG 
GAATATGGCC GAGGAGACCT TGTTGGTGTG GTGGAGACGC TGACCCACCA GGCCAGAGCA 
ACCACAGTGC ATGCTGTCCG GGACTCAGAA CTGGCTAAGC TGCCAGCAGG AGCTCTGACG 
TCTATCAAGC GCAGGTACCC ACAGGTTGTA ACGCGACTGA TTCATCTCTT GGGCGAGAAG 
ATCTTAGGCA GTCTCCAGCA GGGATCTGCA ACAGGTCACC AGCTTGGATT TAACACAGCA 
AGCAGCAAAT GGGACTTGGG GAACCCGCCT GGCAATCTGT CCACAGTGGC AGCACTGCCT 
GCTTCTGAGG ATGTGCCCCT AACCGCCTTT GCTCTGGAGC TCCAACATGC CCTCAGTGCC 
ATCGGTCCTG TCCTGCTGCT GACCAGTGAC AACATCAAAC AACGCCTGGG TTCCGCTGCT 
CTAGACAGCA TCCATGAGTA CCGGCTCTCC AGTTGGCTGG GCCAGCAGGA GGACATCCAC 
CGGATTGTGC TGTATCAGGC AGACGGCACA CTCACACCGT GGACCCAGCG CTGCATCCGG 
CAGGCTGACT GCATCCTCAT CGTGGGCTTG GGTGAGCAAG AGCCAGCAGT AGGTGAGCTG 
GAGCAGATGT TGGAGAGCAC AGCTGTGCGT GCCCAGAAGC AGCTAATCTT GCTGCACAAA 
GAGGACGGTC CAGTGCCTTC CCGCACAGTG GAGTGGCTCA ACATGCGAAG TTGGTGTTCT 
GGCCATTTGC ACCTCTGCTG CCCACGCCGT GTCTTCTCCA AGAGAAGCTT GCCCAAGCTG 
GTCGAGATGT ACACACGTGT TTTCCAACGA CCACCAGACC GTCATTCTGA TTTTTCCCGC 
CTGGCACGGA TGCTGACAGG CAATGCCATT GCCTTGGTGC TTGGAGGGGG TGGAGCTAGA 
GGGTGTGCCC AGGTGGGCAT CCTCAGGGCA CTAGCAGAGT GTGGCGTCCC CGTGGACATA 
ATCGGAGGCA CATCCATCGG GGCTTTCATG GGTGCTCTGT TTGCTGAGGA GCGGAGCTAC 
AGCCAAACAC GGATCCGGGC CAAGCAGTGG GCAGAGGGTA TGACATCAAT GATGAAGACT 
ATACTGGACC TGACCTACCC AATCACATCC ATGTTCTCTG GCACTGGCTT CAATAGCAGC 
ATCAGCAACA TCTTCAAAGA CCGGCAGATT GAGGACCTGT GGCTCCCCTA CTTTGCCATC 
ACCACCGACA TCACGGCCTC TGCCATGCGG GTTCACACAG ATGGCTCTCT GTGGAGGTAT 
GTCCGTGCAA GCATGTCCTT GTCAGGCTAT ATGCCCCCAC TCTGCGACCC CAAGGATGGA 
CACCTGCTGA TGGATGGTGG TTACATCAAC AACCTCCCAG CGGATGTGGC AAGATCCATG 
GGGGCAAAGG TTGTGATTGC CATCGATGTT GGCAGCCGGG ACGAGACAGA CCTCACCAAC 
TATGGCGATG CCTTGTCTGG ATGGTGGCTG CTTTGGAAAC GCTGGAACCC CTTGGCTACA 
AAAGTCAAGG TGTTGAACAT GGCAGAGATT CAGACTCGAC TGGCCTATGT GTGCTGCGTC 
CGGCAGCTGG AGATGGTGAA GAACAGCGAC TACTGTGAGT ACCTGAGGCC TCCCATCGAC 
AGTTACCGCA CCCTGGACTT CGGCAAATTT GATGAGATCT GTGAAGTTGG GTATCAACAT 
GGGCGGACAG TGTTTGACAT CTGGGTCCGG AGTGGTGTCC TAGAGAAGAT GTTGCAGGAC 
CAGCAGGGGA CAAGCAAGAG GAAGGACTGT GGGGTGTTCA CCTGCCCCAA TTCCTCCTTC 
ACGGACCTTG CTGAGATTGT GTCACGGATT GAGCCGGCCA AGGTGGCTGC AGTGGATGCC 
TTGCTTGGCA CAGCCACTGT CCTTGGAGAG AGGACCAATG GAGCCCTCTC TTCTGCTCTA 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGAATA TGAGCCTTCG 
ATGTTGCAAG GACCCCCCAG CTTGACTTCC CCAGAACAGT CCCAGGACTC CTTCCCTTGG 
CTCCCTAATC AAGATGACCA GGGGCCAAGA CTGGAACATC CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498085.1
CDS	139..4026
Translation	MFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498022.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498022.2

ORF Insert Sequence:

ATGTTTTATG GCCGGAAGAT CATGAGGAAG GTGACCACAC TCCCCCATAC CTTGGTTGGG 
AATACCTCTG CACCCCGGCA GCGAGTCAGG AAGAGGACCA AAGTGCTGTC TTTAGCCAAG 
AGGATTCTGC GCTTTAAGAA AGAGTATCCC ACTTTGCAGC CAAAAGAGCC TCCGCCCTCC 
CTGCTTGAGG CTGATCTTAC AGAGTTTGAT GTGAAGAACT CTCATCTGCC ATCAGAAGTC 
TTGTACATGT TGAAAAATGT TCGGGTTCTA GGCCACTTTG AGAAGCCACT ATTTCTGGAA 
CTTTGCAAAC ATATGGTCTT TGTGCAGCTA CAAGAAGGGG AGCACGTATT CCAACCTGGG 
GAGCCTGACA TCAGCATCTA TGTGGTTCAG GACGGACGGC TGGAGGTCTG CATCCAAGAC 
GCTGTGACAC AGGATGGCAC CGAAGTGGTG GTCAAAGAGG TCCTGCCAGG GGACAGTGTC 
CACAGTCTCC TCAGCATTTT GGATGTGATC ACCGGTCACA CTGCACCATA CAAAACAGTT 
TCTGCCCGTG CCGCTGTCTC GTCCACCGTG CTCTGGCTTC CTGCAGCAGC TTTTCAAGGA 
GTGTTTGAGA AATACCCTGA AACTCTGGTC AGGGTTGTGC AGATCATCAT GGTCCGGCTG 
CAGCGGGTGA CTTTCCTGGC TCTGCACAAC TACCTGGGGC TGACTACAGA GCTCTTCAAC 
CCTGAGAGCC AGGCCATCCC TCTCTTGTCA GTAGCAAGTG TGGCTGGACG AGCCAAGAGA 
CAGATGTCAT ATGGTCCAGA GGAGCAGCTT GAGAGGTCCC TACGGCCCTC GGAGTTCAGC 
AGCTCAGATC ATGGGAGCAG CTGTGTGACA GTCTCTGGGC CTCTGCTGAA GAGGAGCTGC 
TCAGTGCCTC TGCCTTCTAA CCATGGGGAG GTGGATGAGC TCAGACAGTC CCAGGGAAGT 
GGCTCTAACA CTTCTGCCTT CCAAGAAAGC CATGAGGGAG CCACCTCAGA CCTGGGGATG 
GCATACAACC GTGCCAGGAT TTTGCCACAC TCTGATGAGC AACTTGGGAA CTCTTTGGCC 
AGCAAGTCCA AGAAAAGTGT TGTCGCAGAG ACCCCCTCTG CCATCTTCCA CTACTCAGAA 
AATTTCAGGG ATGAGACTGG GGCCTGTGGG AAGACAGATG CTATCTTCAG AGCTGCCACA 
AAGGACCTCC TTACCCTGAT GAAGCTAGAT GACCCATCCC TGTTGGATGG CCGGGTGGCA 
TTTCTTCATG TTCCTGCAGG CACCTTAGTG TCAAAACAGG GAGATCAGGA TGTCAATATT 
TTGTTTGTGG TCTCGGGGAT GCTGCATGTG TACCAGCAAA AGATTGACAG CTTGGAGGAT 
ACTTGCCTGT TCCTCACACA TCCCGGGGAG ATGGTGGGTC AGCTGGCGGT GCTCACTGGA 
GAACCCCTAA TGTTCACCAT CAGAGCCAAC CGAGACTGCA GCTTTTTGTC CATCTCGAAG 
GCCCACTTCT ATGAGATCAT GAGGAAACGG CCGGATGTTG TCCTGGGCGT GGCACACACA 
GTTGTGAAGA GGATGTCATC CTTTGTACGG CAGATTGATT TTGCTCTAGA CTGGATGGAA 
GTAGAGGCTG GACGTGCCAT ATACAGGCAA GGAGACAAGT CTGACTGCAC ATACATCGTT 
CTCAGTGGCA GGCTGCGTTC TGTCATCCGA AAAGATGATG GGAAAAAACG CCTGGCAGGG 
GAATATGGCC GAGGAGACCT TGTTGGTGTG GTGGAGACGC TGACCCACCA GGCCAGAGCA 
ACCACAGTGC ATGCTGTCCG GGACTCAGAA CTGGCTAAGC TGCCAGCAGG AGCTCTGACG 
TCTATCAAGC GCAGGTACCC ACAGGTTGTA ACGCGACTGA TTCATCTCTT GGGCGAGAAG 
ATCTTAGGCA GTCTCCAGCA GGGATCTGCA ACAGGTCACC AGCTTGGATT TAACACAGCA 
AGCAGCAAAT GGGACTTGGG GAACCCGCCT GGCAATCTGT CCACAGTGGC AGCACTGCCT 
GCTTCTGAGG ATGTGCCCCT AACCGCCTTT GCTCTGGAGC TCCAACATGC CCTCAGTGCC 
ATCGGTCCTG TCCTGCTGCT GACCAGTGAC AACATCAAAC AACGCCTGGG TTCCGCTGCT 
CTAGACAGCA TCCATGAGTA CCGGCTCTCC AGTTGGCTGG GCCAGCAGGA GGACATCCAC 
CGGATTGTGC TGTATCAGGC AGACGGCACA CTCACACCGT GGACCCAGCG CTGCATCCGG 
CAGGCTGACT GCATCCTCAT CGTGGGCTTG GGTGAGCAAG AGCCAGCAGT AGGTGAGCTG 
GAGCAGATGT TGGAGAGCAC AGCTGTGCGT GCCCAGAAGC AGCTAATCTT GCTGCACAAA 
GAGGACGGTC CAGTGCCTTC CCGCACAGTG GAGTGGCTCA ACATGCGAAG TTGGTGTTCT 
GGCCATTTGC ACCTCTGCTG CCCACGCCGT GTCTTCTCCA AGAGAAGCTT GCCCAAGCTG 
GTCGAGATGT ACACACGTGT TTTCCAACGA CCACCAGACC GTCATTCTGA TTTTTCCCGC 
CTGGCACGGA TGCTGACAGG CAATGCCATT GCCTTGGTGC TTGGAGGGGG TGGAGCTAGA 
GGGTGTGCCC AGGTGGGCAT CCTCAGGGCA CTAGCAGAGT GTGGCGTCCC CGTGGACATA 
ATCGGAGGCA CATCCATCGG GGCTTTCATG GGTGCTCTGT TTGCTGAGGA GCGGAGCTAC 
AGCCAAACAC GGATCCGGGC CAAGCAGTGG GCAGAGGGTA TGACATCAAT GATGAAGACT 
ATACTGGACC TGACCTACCC AATCACATCC ATGTTCTCTG GCACTGGCTT CAATAGCAGC 
ATCAGCAACA TCTTCAAAGA CCGGCAGATT GAGGACCTGT GGCTCCCCTA CTTTGCCATC 
ACCACCGACA TCACGGCCTC TGCCATGCGG GTTCACACAG ATGGCTCTCT GTGGAGGTAT 
GTCCGTGCAA GCATGTCCTT GTCAGGCTAT ATGCCCCCAC TCTGCGACCC CAAGGATGGA 
CACCTGCTGA TGGATGGTGG TTACATCAAC AACCTCCCAG CGGATGTGGC AAGATCCATG 
GGGGCAAAGG TTGTGATTGC CATCGATGTT GGCAGCCGGG ACGAGACAGA CCTCACCAAC 
TATGGCGATG CCTTGTCTGG ATGGTGGCTG CTTTGGAAAC GCTGGAACCC CTTGGCTACA 
AAAGTCAAGG TGTTGAACAT GGCAGAGATT CAGACTCGAC TGGCCTATGT GTGCTGCGTC 
CGGCAGCTGG AGATGGTGAA GAACAGCGAC TACTGTGAGT ACCTGAGGCC TCCCATCGAC 
AGTTACCGCA CCCTGGACTT CGGCAAATTT GATGAGATCT GTGAAGTTGG GTATCAACAT 
GGGCGGACAG TGTTTGACAT CTGGGTCCGG AGTGGTGTCC TAGAGAAGAT GTTGCAGGAC 
CAGCAGGGGA CAAGCAAGAG GAAGGACTGT GGGGTGTTCA CCTGCCCCAA TTCCTCCTTC 
ACGGACCTTG CTGAGATTGT GTCACGGATT GAGCCGGCCA AGGTGGCTGC AGTGGATGCC 
TTGCTTGGCA CAGCCACTGT CCTTGGAGAG AGGACCAATG GAGCCCTCTC TTCTGCTCTA 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGAATA TGAGCCTTCG 
ATGTTGCAAG GACCCCCCAG CTTGACTTCC CCAGAACAGT CCCAGGACTC CTTCCCTTGG 
CTCCCTAATC AAGATGACCA GGGGCCAAGA CTGGAACATC CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498023.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498086.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X13, mRNA.

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CloneID	OMu29544
Clone ID Related Accession (Same CDS sequence)	XM_006498023.3 , XM_006498023.4
Accession Version	XM_006498023.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498023.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTATG GCCGGAAGAT CATGAGGAAG GTGACCACAC TCCCCCATAC CTTGGTTGGG 
AATACCTCTG CACCCCGGCA GCGAGTCAGG AAGAGGACCA AAGTGCTGTC TTTAGCCAAG 
AGGATTCTGC GCTTTAAGAA AGAGTATCCC ACTTTGCAGC CAAAAGAGCC TCCGCCCTCC 
CTGCTTGAGG CTGATCTTAC AGAGTTTGAT GTGAAGAACT CTCATCTGCC ATCAGAAGTC 
TTGTACATGT TGAAAAATGT TCGGGTTCTA GGCCACTTTG AGAAGCCACT ATTTCTGGAA 
CTTTGCAAAC ATATGGTCTT TGTGCAGCTA CAAGAAGGGG AGCACGTATT CCAACCTGGG 
GAGCCTGACA TCAGCATCTA TGTGGTTCAG GACGGACGGC TGGAGGTCTG CATCCAAGAC 
GCTGATGGCA CCGAAGTGGT GGTCAAAGAG GTCCTGCCAG GGGACAGTGT CCACAGTCTC 
CTCAGCATTT TGGATGTGAT CACCGGTCAC ACTGCACCAT ACAAAACAGT TTCTGCCCGT 
GCCGCTGTCT CGTCCACCGT GCTCTGGCTT CCTGCAGCAG CTTTTCAAGG AGTGTTTGAG 
AAATACCCTG AAACTCTGGT CAGGGTTGTG CAGATCATCA TGGTCCGGCT GCAGCGGGTG 
ACTTTCCTGG CTCTGCACAA CTACCTGGGG CTGACTACAG AGCTCTTCAA CCCTGAGAGC 
CAGGCCATCC CTCTCTTGTC AGTAGCAAGT GTGGCTGGAC GAGCCAAGAG ACAGATGTCA 
TATGGTCCAG AGGAGCAGCT TGAGAGGTCC CTACGGCCCT CGGAGTTCAG CAGCTCAGAT 
CATGGGAGCA GCTGTGTGAC AGTCTCTGGG CCTCTGCTGA AGAGGAGCTG CTCAGTGCCT 
CTGCCTTCTA ACCATGGGGA GGTGGATGAG CTCAGACAGT CCCAGGGAAG TGGCTCTAAC 
ACTTCTGCCT TCCAAGAAAG CCATGAGGGA GCCACCTCAG ACCTGGGGAT GGCATACAAC 
CGTGCCAGGA TTTTGCCACA CTCTGATGAG CAACTTGGGA ACTCTTTGGC CAGCAAGTCC 
AAGAAAAGTG TTGTCGCAGA GACCCCCTCT GCCATCTTCC ACTACTCAGA AAATTTCAGG 
GATGAGACTG GGGCCTGTGG GAAGACAGAT GCTATCTTCA GAGCTGCCAC AAAGGACCTC 
CTTACCCTGA TGAAGCTAGA TGACCCATCC CTGTTGGATG GCCGGGTGGC ATTTCTTCAT 
GTTCCTGCAG GCACCTTAGT GTCAAAACAG GGAGATCAGG ATGTCAATAT TTTGTTTGTG 
GTCTCGGGGA TGCTGCATGT GTACCAGCAA AAGATTGACA GCTTGGAGGA TACTTGCCTG 
TTCCTCACAC ATCCCGGGGA GATGGTGGGT CAGCTGGCGG TGCTCACTGG AGAACCCCTA 
ATGTTCACCA TCAGAGCCAA CCGAGACTGC AGCTTTTTGT CCATCTCGAA GGCCCACTTC 
TATGAGATCA TGAGGAAACG GCCGGATGTT GTCCTGGGCG TGGCACACAC AGTTGTGAAG 
AGGATGTCAT CCTTTGTACG GCAGATTGAT TTTGCTCTAG ACTGGATGGA AGTAGAGGCT 
GGACGTGCCA TATACAGGCA AGGAGACAAG TCTGACTGCA CATACATCGT TCTCAGTGGC 
AGGCTGCGTT CTGTCATCCG AAAAGATGAT GGGAAAAAAC GCCTGGCAGG GGAATATGGC 
CGAGGAGACC TTGTTGGTGT GGTGGAGACG CTGACCCACC AGGCCAGAGC AACCACAGTG 
CATGCTGTCC GGGACTCAGA ACTGGCTAAG CTGCCAGCAG GAGCTCTGAC GTCTATCAAG 
CGCAGGTACC CACAGGTTGT AACGCGACTG ATTCATCTCT TGGGCGAGAA GATCTTAGGC 
AGTCTCCAGC AGGGATCTGC AACAGGTCAC CAGCTTGGAT TTAACACAGC AAGCAGCAAA 
TGGGACTTGG GGAACCCGCC TGGCAATCTG TCCACAGTGG CAGCACTGCC TGCTTCTGAG 
GATGTGCCCC TAACCGCCTT TGCTCTGGAG CTCCAACATG CCCTCAGTGC CATCGGTCCT 
GTCCTGCTGC TGACCAGTGA CAACATCAAA CAACGCCTGG GTTCCGCTGC TCTAGACAGC 
ATCCATGAGT ACCGGCTCTC CAGTTGGCTG GGCCAGCAGG AGGACATCCA CCGGATTGTG 
CTGTATCAGG CAGACGGCAC ACTCACACCG TGGACCCAGC GCTGCATCCG GCAGGCTGAC 
TGCATCCTCA TCGTGGGCTT GGGTGAGCAA GAGCCAGCAG TAGGTGAGCT GGAGCAGATG 
TTGGAGAGCA CAGCTGTGCG TGCCCAGAAG CAGCTAATCT TGCTGCACAA AGAGGACGGT 
CCAGTGCCTT CCCGCACAGT GGAGTGGCTC AACATGCGAA GTTGGTGTTC TGGCCATTTG 
CACCTCTGCT GCCCACGCCG TGTCTTCTCC AAGAGAAGCT TGCCCAAGCT GGTCGAGATG 
TACACACGTG TTTTCCAACG ACCACCAGAC CGTCATTCTG ATTTTTCCCG CCTGGCACGG 
ATGCTGACAG GCAATGCCAT TGCCTTGGTG CTTGGAGGGG GTGGAGCTAG AGGGTGTGCC 
CAGGTGGGCA TCCTCAGGGC ACTAGCAGAG TGTGGCGTCC CCGTGGACAT AATCGGAGGC 
ACATCCATCG GGGCTTTCAT GGGTGCTCTG TTTGCTGAGG AGCGGAGCTA CAGCCAAACA 
CGGATCCGGG CCAAGCAGTG GGCAGAGGGT ATGACATCAA TGATGAAGAC TATACTGGAC 
CTGACCTACC CAATCACATC CATGTTCTCT GGCACTGGCT TCAATAGCAG CATCAGCAAC 
ATCTTCAAAG ACCGGCAGAT TGAGGACCTG TGGCTCCCCT ACTTTGCCAT CACCACCGAC 
ATCACGGCCT CTGCCATGCG GGTTCACACA GATGGCTCTC TGTGGAGGTA TGTCCGTGCA 
AGCATGTCCT TGTCAGGCTA TATGCCCCCA CTCTGCGACC CCAAGGATGG ACACCTGCTG 
ATGGATGGTG GTTACATCAA CAACCTCCCA GCGGATGTGG CAAGATCCAT GGGGGCAAAG 
GTTGTGATTG CCATCGATGT TGGCAGCCGG GACGAGACAG ACCTCACCAA CTATGGCGAT 
GCCTTGTCTG GATGGTGGCT GCTTTGGAAA CGCTGGAACC CCTTGGCTAC AAAAGTCAAG 
GTGTTGAACA TGGCAGAGAT TCAGACTCGA CTGGCCTATG TGTGCTGCGT CCGGCAGCTG 
GAGATGGTGA AGAACAGCGA CTACTGTGAG TACCTGAGGC CTCCCATCGA CAGTTACCGC 
ACCCTGGACT TCGGCAAATT TGATGAGATC TGTGAAGTTG GGTATCAACA TGGGCGGACA 
GTGTTTGACA TCTGGGTCCG GAGTGGTGTC CTAGAGAAGA TGTTGCAGGA CCAGCAGGGG 
ACAAGCAAGA GGAAGGACTG TGGGGTGTTC ACCTGCCCCA ATTCCTCCTT CACGGACCTT 
GCTGAGATTG TGTCACGGAT TGAGCCGGCC AAGGTGGCTG CAGTGGATGC CTTGCTTGGC 
ACAGCCACTG TCCTTGGAGA GAGGACCAAT GGAGCCCTCT CTTCTGCTCT AGATGAGTCT 
GACTATCAGA CCGAGTATGA GGAGGAACTG CCAGCCATCC CCAAGGAAAC GTATGCTGAC 
TTCCAGAGCA CTGGGATTGA GCTAGACTCT GACTCGGAAT ATGAGCCTTC GATGTTGCAA 
GGACCCCCCA GCTTGACTTC CCCAGAACAG TCCCAGGACT CCTTCCCTTG GCTCCCTAAT 
CAAGATGACC AGGGGCCAAG ACTGGAACAT CCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498086.1
CDS	1056..4934
Translation	MFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDADGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498023.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498023.3

ORF Insert Sequence:

ATGTTTTATG GCCGGAAGAT CATGAGGAAG GTGACCACAC TCCCCCATAC CTTGGTTGGG 
AATACCTCTG CACCCCGGCA GCGAGTCAGG AAGAGGACCA AAGTGCTGTC TTTAGCCAAG 
AGGATTCTGC GCTTTAAGAA AGAGTATCCC ACTTTGCAGC CAAAAGAGCC TCCGCCCTCC 
CTGCTTGAGG CTGATCTTAC AGAGTTTGAT GTGAAGAACT CTCATCTGCC ATCAGAAGTC 
TTGTACATGT TGAAAAATGT TCGGGTTCTA GGCCACTTTG AGAAGCCACT ATTTCTGGAA 
CTTTGCAAAC ATATGGTCTT TGTGCAGCTA CAAGAAGGGG AGCACGTATT CCAACCTGGG 
GAGCCTGACA TCAGCATCTA TGTGGTTCAG GACGGACGGC TGGAGGTCTG CATCCAAGAC 
GCTGATGGCA CCGAAGTGGT GGTCAAAGAG GTCCTGCCAG GGGACAGTGT CCACAGTCTC 
CTCAGCATTT TGGATGTGAT CACCGGTCAC ACTGCACCAT ACAAAACAGT TTCTGCCCGT 
GCCGCTGTCT CGTCCACCGT GCTCTGGCTT CCTGCAGCAG CTTTTCAAGG AGTGTTTGAG 
AAATACCCTG AAACTCTGGT CAGGGTTGTG CAGATCATCA TGGTCCGGCT GCAGCGGGTG 
ACTTTCCTGG CTCTGCACAA CTACCTGGGG CTGACTACAG AGCTCTTCAA CCCTGAGAGC 
CAGGCCATCC CTCTCTTGTC AGTAGCAAGT GTGGCTGGAC GAGCCAAGAG ACAGATGTCA 
TATGGTCCAG AGGAGCAGCT TGAGAGGTCC CTACGGCCCT CGGAGTTCAG CAGCTCAGAT 
CATGGGAGCA GCTGTGTGAC AGTCTCTGGG CCTCTGCTGA AGAGGAGCTG CTCAGTGCCT 
CTGCCTTCTA ACCATGGGGA GGTGGATGAG CTCAGACAGT CCCAGGGAAG TGGCTCTAAC 
ACTTCTGCCT TCCAAGAAAG CCATGAGGGA GCCACCTCAG ACCTGGGGAT GGCATACAAC 
CGTGCCAGGA TTTTGCCACA CTCTGATGAG CAACTTGGGA ACTCTTTGGC CAGCAAGTCC 
AAGAAAAGTG TTGTCGCAGA GACCCCCTCT GCCATCTTCC ACTACTCAGA AAATTTCAGG 
GATGAGACTG GGGCCTGTGG GAAGACAGAT GCTATCTTCA GAGCTGCCAC AAAGGACCTC 
CTTACCCTGA TGAAGCTAGA TGACCCATCC CTGTTGGATG GCCGGGTGGC ATTTCTTCAT 
GTTCCTGCAG GCACCTTAGT GTCAAAACAG GGAGATCAGG ATGTCAATAT TTTGTTTGTG 
GTCTCGGGGA TGCTGCATGT GTACCAGCAA AAGATTGACA GCTTGGAGGA TACTTGCCTG 
TTCCTCACAC ATCCCGGGGA GATGGTGGGT CAGCTGGCGG TGCTCACTGG AGAACCCCTA 
ATGTTCACCA TCAGAGCCAA CCGAGACTGC AGCTTTTTGT CCATCTCGAA GGCCCACTTC 
TATGAGATCA TGAGGAAACG GCCGGATGTT GTCCTGGGCG TGGCACACAC AGTTGTGAAG 
AGGATGTCAT CCTTTGTACG GCAGATTGAT TTTGCTCTAG ACTGGATGGA AGTAGAGGCT 
GGACGTGCCA TATACAGGCA AGGAGACAAG TCTGACTGCA CATACATCGT TCTCAGTGGC 
AGGCTGCGTT CTGTCATCCG AAAAGATGAT GGGAAAAAAC GCCTGGCAGG GGAATATGGC 
CGAGGAGACC TTGTTGGTGT GGTGGAGACG CTGACCCACC AGGCCAGAGC AACCACAGTG 
CATGCTGTCC GGGACTCAGA ACTGGCTAAG CTGCCAGCAG GAGCTCTGAC GTCTATCAAG 
CGCAGGTACC CACAGGTTGT AACGCGACTG ATTCATCTCT TGGGCGAGAA GATCTTAGGC 
AGTCTCCAGC AGGGATCTGC AACAGGTCAC CAGCTTGGAT TTAACACAGC AAGCAGCAAA 
TGGGACTTGG GGAACCCGCC TGGCAATCTG TCCACAGTGG CAGCACTGCC TGCTTCTGAG 
GATGTGCCCC TAACCGCCTT TGCTCTGGAG CTCCAACATG CCCTCAGTGC CATCGGTCCT 
GTCCTGCTGC TGACCAGTGA CAACATCAAA CAACGCCTGG GTTCCGCTGC TCTAGACAGC 
ATCCATGAGT ACCGGCTCTC CAGTTGGCTG GGCCAGCAGG AGGACATCCA CCGGATTGTG 
CTGTATCAGG CAGACGGCAC ACTCACACCG TGGACCCAGC GCTGCATCCG GCAGGCTGAC 
TGCATCCTCA TCGTGGGCTT GGGTGAGCAA GAGCCAGCAG TAGGTGAGCT GGAGCAGATG 
TTGGAGAGCA CAGCTGTGCG TGCCCAGAAG CAGCTAATCT TGCTGCACAA AGAGGACGGT 
CCAGTGCCTT CCCGCACAGT GGAGTGGCTC AACATGCGAA GTTGGTGTTC TGGCCATTTG 
CACCTCTGCT GCCCACGCCG TGTCTTCTCC AAGAGAAGCT TGCCCAAGCT GGTCGAGATG 
TACACACGTG TTTTCCAACG ACCACCAGAC CGTCATTCTG ATTTTTCCCG CCTGGCACGG 
ATGCTGACAG GCAATGCCAT TGCCTTGGTG CTTGGAGGGG GTGGAGCTAG AGGGTGTGCC 
CAGGTGGGCA TCCTCAGGGC ACTAGCAGAG TGTGGCGTCC CCGTGGACAT AATCGGAGGC 
ACATCCATCG GGGCTTTCAT GGGTGCTCTG TTTGCTGAGG AGCGGAGCTA CAGCCAAACA 
CGGATCCGGG CCAAGCAGTG GGCAGAGGGT ATGACATCAA TGATGAAGAC TATACTGGAC 
CTGACCTACC CAATCACATC CATGTTCTCT GGCACTGGCT TCAATAGCAG CATCAGCAAC 
ATCTTCAAAG ACCGGCAGAT TGAGGACCTG TGGCTCCCCT ACTTTGCCAT CACCACCGAC 
ATCACGGCCT CTGCCATGCG GGTTCACACA GATGGCTCTC TGTGGAGGTA TGTCCGTGCA 
AGCATGTCCT TGTCAGGCTA TATGCCCCCA CTCTGCGACC CCAAGGATGG ACACCTGCTG 
ATGGATGGTG GTTACATCAA CAACCTCCCA GCGGATGTGG CAAGATCCAT GGGGGCAAAG 
GTTGTGATTG CCATCGATGT TGGCAGCCGG GACGAGACAG ACCTCACCAA CTATGGCGAT 
GCCTTGTCTG GATGGTGGCT GCTTTGGAAA CGCTGGAACC CCTTGGCTAC AAAAGTCAAG 
GTGTTGAACA TGGCAGAGAT TCAGACTCGA CTGGCCTATG TGTGCTGCGT CCGGCAGCTG 
GAGATGGTGA AGAACAGCGA CTACTGTGAG TACCTGAGGC CTCCCATCGA CAGTTACCGC 
ACCCTGGACT TCGGCAAATT TGATGAGATC TGTGAAGTTG GGTATCAACA TGGGCGGACA 
GTGTTTGACA TCTGGGTCCG GAGTGGTGTC CTAGAGAAGA TGTTGCAGGA CCAGCAGGGG 
ACAAGCAAGA GGAAGGACTG TGGGGTGTTC ACCTGCCCCA ATTCCTCCTT CACGGACCTT 
GCTGAGATTG TGTCACGGAT TGAGCCGGCC AAGGTGGCTG CAGTGGATGC CTTGCTTGGC 
ACAGCCACTG TCCTTGGAGA GAGGACCAAT GGAGCCCTCT CTTCTGCTCT AGATGAGTCT 
GACTATCAGA CCGAGTATGA GGAGGAACTG CCAGCCATCC CCAAGGAAAC GTATGCTGAC 
TTCCAGAGCA CTGGGATTGA GCTAGACTCT GACTCGGAAT ATGAGCCTTC GATGTTGCAA 
GGACCCCCCA GCTTGACTTC CCCAGAACAG TCCCAGGACT CCTTCCCTTG GCTCCCTAAT 
CAAGATGACC AGGGGCCAAG ACTGGAACAT CCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498024.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498087.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X15, mRNA.

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CloneID	OMu29545
Clone ID Related Accession (Same CDS sequence)	XM_006498024.1
Accession Version	XM_006498024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGGC TGCAGCGGGT GACTTTCCTG GCTCTGCACA ACTACCTGGG GCTGACTACA 
GAGCTCTTCA ACCCTGAGAG CCAGGCCATC CCTCTCTTGT CAGTAGCAAG TGTGGCTGGA 
CGAGCCAAGA GACAGATGTC ATATGGTCCA GAGGAGCAGC TTGAGAGGTC CCTACGGCCC 
TCGGAGTTCA GCAGCTCAGA TCATGGGAGC AGCTGTGTGA CAGTCTCTGG GCCTCTGCTG 
AAGAGGAGCT GCTCAGTGCC TCTGCCTTCT AACCATGGGG AGGTGGATGA GCTCAGACAG 
TCCCAGGGAA GTGGCTCTAA CACTTCTGCC TTCCAAGAAA GCCATGAGGG AGCCACCTCA 
GACCTGGGGA TGGCATACAA CCGTGCCAGG ATTTTGCCAC ACTCTGATGA GCAACTTGGG 
AACTCTTTGG CCAGCAAGTC CAAGAAAAGT GTTGTCGCAG AGACCCCCTC TGCCATCTTC 
CACTACTCAG AAAATTTCAG GGATGAGACT GGGGCCTGTG GGAAGACAGA TGCTATCTTC 
AGAGCTGCCA CAAAGGACCT CCTTACCCTG ATGAAGCTAG ATGACCCATC CCTGTTGGAT 
GGCCGGGTGG CATTTCTTCA TGTTCCTGCA GGCACCTTAG TGTCAAAACA GGGAGATCAG 
GATGTCAATA TTTTGTTTGT GGTCTCGGGG ATGCTGCATG TGTACCAGCA AAAGATTGAC 
AGCTTGGAGG ATACTTGCCT GTTCCTCACA CATCCCGGGG AGATGGTGGG TCAGCTGGCG 
GTGCTCACTG GAGAACCCCT AATGTTCACC ATCAGAGCCA ACCGAGACTG CAGCTTTTTG 
TCCATCTCGA AGGCCCACTT CTATGAGATC ATGAGGAAAC GGCCGGATGT TGTCCTGGGC 
GTGGCACACA CAGTTGTGAA GAGGATGTCA TCCTTTGTAC GGCAGATTGA TTTTGCTCTA 
GACTGGATGG AAGTAGAGGC TGGACGTGCC ATATACAGGC AAGGAGACAA GTCTGACTGC 
ACATACATCG TTCTCAGTGG CAGGCTGCGT TCTGTCATCC GAAAAGATGA TGGGAAAAAA 
CGCCTGGCAG GGGAATATGG CCGAGGAGAC CTTGTTGGTG TGGTGGAGAC GCTGACCCAC 
CAGGCCAGAG CAACCACAGT GCATGCTGTC CGGGACTCAG AACTGGCTAA GCTGCCAGCA 
GGAGCTCTGA CGTCTATCAA GCGCAGGTAC CCACAGGTTG TAACGCGACT GATTCATCTC 
TTGGGCGAGA AGATCTTAGG CAGTCTCCAG CAGGGATCTG CAACAGGTCA CCAGCTTGGA 
TTTAACACAG CAAGCAGCAA ATGGGACTTG GGGAACCCGC CTGGCAATCT GTCCACAGTG 
GCAGCACTGC CTGCTTCTGA GGATGTGCCC CTAACCGCCT TTGCTCTGGA GCTCCAACAT 
GCCCTCAGTG CCATCGGTCC TGTCCTGCTG CTGACCAGTG ACAACATCAA ACAACGCCTG 
GGTTCCGCTG CTCTAGACAG CATCCATGAG TACCGGCTCT CCAGTTGGCT GGGCCAGCAG 
GAGGACATCC ACCGGATTGT GCTGTATCAG GCAGACGGCA CACTCACACC GTGGACCCAG 
CGCTGCATCC GGCAGGCTGA CTGCATCCTC ATCGTGGGCT TGGGTGAGCA AGAGCCAGCA 
GTAGGTGAGC TGGAGCAGAT GTTGGAGAGC ACAGCTGTGC GTGCCCAGAA GCAGCTAATC 
TTGCTGCACA AAGAGGACGG TCCAGTGCCT TCCCGCACAG TGGAGTGGCT CAACATGCGA 
AGTTGGTGTT CTGGCCATTT GCACCTCTGC TGCCCACGCC GTGTCTTCTC CAAGAGAAGC 
TTGCCCAAGC TGGTCGAGAT GTACACACGT GTTTTCCAAC GACCACCAGA CCGTCATTCT 
GATTTTTCCC GCCTGGCACG GATGCTGACA GGCAATGCCA TTGCCTTGGT GCTTGGAGGG 
GGTGGAGCTA GAGGGTGTGC CCAGGTGGGC ATCCTCAGGG CACTAGCAGA GTGTGGCGTC 
CCCGTGGACA TAATCGGAGG CACATCCATC GGGGCTTTCA TGGGTGCTCT GTTTGCTGAG 
GAGCGGAGCT ACAGCCAAAC ACGGATCCGG GCCAAGCAGT GGGCAGAGGG TATGACATCA 
ATGATGAAGA CTATACTGGA CCTGACCTAC CCAATCACAT CCATGTTCTC TGGCACTGGC 
TTCAATAGCA GCATCAGCAA CATCTTCAAA GACCGGCAGA TTGAGGACCT GTGGCTCCCC 
TACTTTGCCA TCACCACCGA CATCACGGCC TCTGCCATGC GGGTTCACAC AGATGGCTCT 
CTGTGGAGGT ATGTCCGTGC AAGCATGTCC TTGTCAGGCT ATATGCCCCC ACTCTGCGAC 
CCCAAGGATG GACACCTGCT GATGGATGGT GGTTACATCA ACAACCTCCC AGCGGATGTG 
GCAAGATCCA TGGGGGCAAA GGTTGTGATT GCCATCGATG TTGGCAGCCG GGACGAGACA 
GACCTCACCA ACTATGGCGA TGCCTTGTCT GGATGGTGGC TGCTTTGGAA ACGCTGGAAC 
CCCTTGGCTA CAAAAGTCAA GGTGTTGAAC ATGGCAGAGA TTCAGACTCG ACTGGCCTAT 
GTGTGCTGCG TCCGGCAGCT GGAGATGGTG AAGAACAGCG ACTACTGTGA GTACCTGAGG 
CCTCCCATCG ACAGTTACCG CACCCTGGAC TTCGGCAAAT TTGATGAGAT CTGTGAAGTT 
GGGTATCAAC ATGGGCGGAC AGTGTTTGAC ATCTGGGTCC GGAGTGGTGT CCTAGAGAAG 
ATGTTGCAGG ACCAGCAGGG GACAAGCAAG AGGAAGGACT GTGGGGTGTT CACCTGCCCC 
AATTCCTCCT TCACGGACCT TGCTGAGATT GTGTCACGGA TTGAGCCGGC CAAGGTGGCT 
GCAGTGGATG CCTTGCTTGG CACAGCCACT GTCCTTGGAG AGAGGACCAA TGGAGCCCTC 
TCTTCTGCTC TAGATGAGTC TGACTATCAG ACCGAGTATG AGGAGGAACT GCCAGCCATC 
CCCAAGGAAA CGTATGCTGA CTTCCAGAGC ACTGGGATTG AGCTAGACTC TGACTCGGAA 
TATGAGCCTT CGATGTTGCA AGGACCCCCC AGCTTGACTT CCCCAGAACA GTCCCAGGAC 
TCCTTCCCTT GGCTCCCTAA TCAAGATGAC CAGGGGCCAA GACTGGAACA TCCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498087.1
CDS	433..3672
Translation	MVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498024.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498024.1

ORF Insert Sequence:

ATGGTCCGGC TGCAGCGGGT GACTTTCCTG GCTCTGCACA ACTACCTGGG GCTGACTACA 
GAGCTCTTCA ACCCTGAGAG CCAGGCCATC CCTCTCTTGT CAGTAGCAAG TGTGGCTGGA 
CGAGCCAAGA GACAGATGTC ATATGGTCCA GAGGAGCAGC TTGAGAGGTC CCTACGGCCC 
TCGGAGTTCA GCAGCTCAGA TCATGGGAGC AGCTGTGTGA CAGTCTCTGG GCCTCTGCTG 
AAGAGGAGCT GCTCAGTGCC TCTGCCTTCT AACCATGGGG AGGTGGATGA GCTCAGACAG 
TCCCAGGGAA GTGGCTCTAA CACTTCTGCC TTCCAAGAAA GCCATGAGGG AGCCACCTCA 
GACCTGGGGA TGGCATACAA CCGTGCCAGG ATTTTGCCAC ACTCTGATGA GCAACTTGGG 
AACTCTTTGG CCAGCAAGTC CAAGAAAAGT GTTGTCGCAG AGACCCCCTC TGCCATCTTC 
CACTACTCAG AAAATTTCAG GGATGAGACT GGGGCCTGTG GGAAGACAGA TGCTATCTTC 
AGAGCTGCCA CAAAGGACCT CCTTACCCTG ATGAAGCTAG ATGACCCATC CCTGTTGGAT 
GGCCGGGTGG CATTTCTTCA TGTTCCTGCA GGCACCTTAG TGTCAAAACA GGGAGATCAG 
GATGTCAATA TTTTGTTTGT GGTCTCGGGG ATGCTGCATG TGTACCAGCA AAAGATTGAC 
AGCTTGGAGG ATACTTGCCT GTTCCTCACA CATCCCGGGG AGATGGTGGG TCAGCTGGCG 
GTGCTCACTG GAGAACCCCT AATGTTCACC ATCAGAGCCA ACCGAGACTG CAGCTTTTTG 
TCCATCTCGA AGGCCCACTT CTATGAGATC ATGAGGAAAC GGCCGGATGT TGTCCTGGGC 
GTGGCACACA CAGTTGTGAA GAGGATGTCA TCCTTTGTAC GGCAGATTGA TTTTGCTCTA 
GACTGGATGG AAGTAGAGGC TGGACGTGCC ATATACAGGC AAGGAGACAA GTCTGACTGC 
ACATACATCG TTCTCAGTGG CAGGCTGCGT TCTGTCATCC GAAAAGATGA TGGGAAAAAA 
CGCCTGGCAG GGGAATATGG CCGAGGAGAC CTTGTTGGTG TGGTGGAGAC GCTGACCCAC 
CAGGCCAGAG CAACCACAGT GCATGCTGTC CGGGACTCAG AACTGGCTAA GCTGCCAGCA 
GGAGCTCTGA CGTCTATCAA GCGCAGGTAC CCACAGGTTG TAACGCGACT GATTCATCTC 
TTGGGCGAGA AGATCTTAGG CAGTCTCCAG CAGGGATCTG CAACAGGTCA CCAGCTTGGA 
TTTAACACAG CAAGCAGCAA ATGGGACTTG GGGAACCCGC CTGGCAATCT GTCCACAGTG 
GCAGCACTGC CTGCTTCTGA GGATGTGCCC CTAACCGCCT TTGCTCTGGA GCTCCAACAT 
GCCCTCAGTG CCATCGGTCC TGTCCTGCTG CTGACCAGTG ACAACATCAA ACAACGCCTG 
GGTTCCGCTG CTCTAGACAG CATCCATGAG TACCGGCTCT CCAGTTGGCT GGGCCAGCAG 
GAGGACATCC ACCGGATTGT GCTGTATCAG GCAGACGGCA CACTCACACC GTGGACCCAG 
CGCTGCATCC GGCAGGCTGA CTGCATCCTC ATCGTGGGCT TGGGTGAGCA AGAGCCAGCA 
GTAGGTGAGC TGGAGCAGAT GTTGGAGAGC ACAGCTGTGC GTGCCCAGAA GCAGCTAATC 
TTGCTGCACA AAGAGGACGG TCCAGTGCCT TCCCGCACAG TGGAGTGGCT CAACATGCGA 
AGTTGGTGTT CTGGCCATTT GCACCTCTGC TGCCCACGCC GTGTCTTCTC CAAGAGAAGC 
TTGCCCAAGC TGGTCGAGAT GTACACACGT GTTTTCCAAC GACCACCAGA CCGTCATTCT 
GATTTTTCCC GCCTGGCACG GATGCTGACA GGCAATGCCA TTGCCTTGGT GCTTGGAGGG 
GGTGGAGCTA GAGGGTGTGC CCAGGTGGGC ATCCTCAGGG CACTAGCAGA GTGTGGCGTC 
CCCGTGGACA TAATCGGAGG CACATCCATC GGGGCTTTCA TGGGTGCTCT GTTTGCTGAG 
GAGCGGAGCT ACAGCCAAAC ACGGATCCGG GCCAAGCAGT GGGCAGAGGG TATGACATCA 
ATGATGAAGA CTATACTGGA CCTGACCTAC CCAATCACAT CCATGTTCTC TGGCACTGGC 
TTCAATAGCA GCATCAGCAA CATCTTCAAA GACCGGCAGA TTGAGGACCT GTGGCTCCCC 
TACTTTGCCA TCACCACCGA CATCACGGCC TCTGCCATGC GGGTTCACAC AGATGGCTCT 
CTGTGGAGGT ATGTCCGTGC AAGCATGTCC TTGTCAGGCT ATATGCCCCC ACTCTGCGAC 
CCCAAGGATG GACACCTGCT GATGGATGGT GGTTACATCA ACAACCTCCC AGCGGATGTG 
GCAAGATCCA TGGGGGCAAA GGTTGTGATT GCCATCGATG TTGGCAGCCG GGACGAGACA 
GACCTCACCA ACTATGGCGA TGCCTTGTCT GGATGGTGGC TGCTTTGGAA ACGCTGGAAC 
CCCTTGGCTA CAAAAGTCAA GGTGTTGAAC ATGGCAGAGA TTCAGACTCG ACTGGCCTAT 
GTGTGCTGCG TCCGGCAGCT GGAGATGGTG AAGAACAGCG ACTACTGTGA GTACCTGAGG 
CCTCCCATCG ACAGTTACCG CACCCTGGAC TTCGGCAAAT TTGATGAGAT CTGTGAAGTT 
GGGTATCAAC ATGGGCGGAC AGTGTTTGAC ATCTGGGTCC GGAGTGGTGT CCTAGAGAAG 
ATGTTGCAGG ACCAGCAGGG GACAAGCAAG AGGAAGGACT GTGGGGTGTT CACCTGCCCC 
AATTCCTCCT TCACGGACCT TGCTGAGATT GTGTCACGGA TTGAGCCGGC CAAGGTGGCT 
GCAGTGGATG CCTTGCTTGG CACAGCCACT GTCCTTGGAG AGAGGACCAA TGGAGCCCTC 
TCTTCTGCTC TAGATGAGTC TGACTATCAG ACCGAGTATG AGGAGGAACT GCCAGCCATC 
CCCAAGGAAA CGTATGCTGA CTTCCAGAGC ACTGGGATTG AGCTAGACTC TGACTCGGAA 
TATGAGCCTT CGATGTTGCA AGGACCCCCC AGCTTGACTT CCCCAGAACA GTCCCAGGAC 
TCCTTCCCTT GGCTCCCTAA TCAAGATGAC CAGGGGCCAA GACTGGAACA TCCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( NM_146251.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666363.3 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), mRNA.

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CloneID	OMu15337
Clone ID Related Accession (Same CDS sequence)	NM_146251.4
Accession Version	NM_146251.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB852360.1, BB618610.1, BC141366.1, BE623320.1 and AI505242.1. On Mar 17, 2009 this sequence version replaced NM_146251.3. ##Evidence-Data-START## Transcript exon combination :: AK148380.1, AK088362.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGATG GCACCGAAGT GGTGGTCAAA GAGGTCCTGC CAGGGGACAG TGTCCACAGT 
CTCCTCAGCA TTTTGGATGT GATCACCGGT CACACTGCAC CATACAAAAC AGTTTCTGCC 
CGTGCCGCTG TCTCGTCCAC CGTGCTCTGG CTTCCTGCAG CAGCTTTTCA AGGAGTGTTT 
GAGAAATACC CTGAAACTCT GGTCAGGGTT GTGCAGATCA TCATGGTCCG GCTGCAGCGG 
GTGACTTTCC TGGCTCTGCA CAACTACCTG GGGCTGACTA CAGAGCTCTT CAACCCTGAG 
AGCCAGGCCA TCCCTCTCTT GTCAGTAGCA AGTGTGGCTG GACGAGCCAA GAGACAGATG 
TCATATGGTC CAGAGGAGCA GCTTGAGAGG TCCCTACGGC CCTCGGAGTT CAGCAGCTCA 
GATCATGGGA GCAGCTGTGT GACAGTCTCT GGGCCTCTGC TGAAGAGGAG CTGCTCAGTG 
CCTCTGCCTT CTAACCATGG GGAGGTGGAT GAGCTCAGAC AGTCCCAGGG AAGTGGCTCT 
AACACTTCTG CCTTCCAAGA AAGCCATGAG GGAGCCACCT CAGACCTGGG GATGGCATAC 
AACCGTGCCA GGATTTTGCC ACACTCTGAT GAGCAACTTG GGAACTCTTT GGCCAGCAAG 
TCCAAGAAAA GTGTTGTCGC AGAGACCCCC TCTGCCATCT TCCACTACTC AGAAAATTTC 
AGGGATGAGA CTGGGGCCTG TGGGAAGACA GATGCTATCT TCAGAGCTGC CACAAAGGAC 
CTCCTTACCC TGATGAAGCT AGATGACCCA TCCCTGTTGG ATGGCCGGGT GGCATTTCTT 
CATGTTCCTG CAGGCACCTT AGTGTCAAAA CAGGGAGATC AGGATGTCAA TATTTTGTTT 
GTGGTCTCGG GGATGCTGCA TGTGTACCAG CAAAAGATTG ACAGCTTGGA GGATACTTGC 
CTGTTCCTCA CACATCCCGG GGAGATGGTG GGTCAGCTGG CGGTGCTCAC TGGAGAACCC 
CTAATGTTCA CCATCAGAGC CAACCGAGAC TGCAGCTTTT TGTCCATCTC GAAGGCCCAC 
TTCTATGAGA TCATGAGGAA ACGGCCGGAT GTTGTCCTGG GCGTGGCACA CACAGTTGTG 
AAGAGGATGT CATCCTTTGT ACGGCAGATT GATTTTGCTC TAGACTGGAT GGAAGTAGAG 
GCTGGACGTG CCATATACAG GCAAGGAGAC AAGTCTGACT GCACATACAT CGTTCTCAGT 
GGCAGGCTGC GTTCTGTCAT CCGAAAAGAT GATGGGAAAA AACGCCTGGC AGGGGAATAT 
GGCCGAGGAG ACCTTGTTGG TGTGGTGGAG ACGCTGACCC ACCAGGCCAG AGCAACCACA 
GTGCATGCTG TCCGGGACTC AGAACTGGCT AAGCTGCCAG CAGGAGCTCT GACGTCTATC 
AAGCGCAGGT ACCCACAGGT TGTAACGCGA CTGATTCATC TCTTGGGCGA GAAGATCTTA 
GGCAGTCTCC AGCAGGGATC TGCAACAGGT CACCAGCTTG GATTTAACAC AGCAAGCAGC 
AAATGGGACT TGGGGAACCC GCCTGGCAAT CTGTCCACAG TGGCAGCACT GCCTGCTTCT 
GAGGATGTGC CCCTAACCGC CTTTGCTCTG GAGCTCCAAC ATGCCCTCAG TGCCATCGGT 
CCTGTCCTGC TGCTGACCAG TGACAACATC AAACAACGCC TGGGTTCCGC TGCTCTAGAC 
AGCATCCATG AGTACCGGCT CTCCAGTTGG CTGGGCCAGC AGGAGGACAT CCACCGGATT 
GTGCTGTATC AGGCAGACGG CACACTCACA CCGTGGACCC AGCGCTGCAT CCGGCAGGCT 
GACTGCATCC TCATCGTGGG CTTGGGTGAG CAAGAGCCAG CAGTAGGTGA GCTGGAGCAG 
ATGTTGGAGA GCACAGCTGT GCGTGCCCAG AAGCAGCTAA TCTTGCTGCA CAAAGAGGAC 
GGTCCAGTGC CTTCCCGCAC AGTGGAGTGG CTCAACATGC GAAGTTGGTG TTCTGGCCAT 
TTGCACCTCT GCTGCCCACG CCGTGTCTTC TCCAAGAGAA GCTTGCCCAA GCTGGTCGAG 
ATGTACACAC GTGTTTTCCA ACGACCACCA GACCGTCATT CTGATTTTTC CCGCCTGGCA 
CGGATGCTGA CAGGCAATGC CATTGCCTTG GTGCTTGGAG GGGGTGGAGC TAGAGGGTGT 
GCCCAGGTGG GCATCCTCAG GGCACTAGCA GAGTGTGGCG TCCCCGTGGA CATAATCGGA 
GGCACATCCA TCGGGGCTTT CATGGGTGCT CTGTTTGCTG AGGAGCGGAG CTACAGCCAA 
ACACGGATCC GGGCCAAGCA GTGGGCAGAG GGTATGACAT CAATGATGAA GACTATACTG 
GACCTGACCT ACCCAATCAC ATCCATGTTC TCTGGCACTG GCTTCAATAG CAGCATCAGC 
AACATCTTCA AAGACCGGCA GATTGAGGAC CTGTGGCTCC CCTACTTTGC CATCACCACC 
GACATCACGG CCTCTGCCAT GCGGGTTCAC ACAGATGGCT CTCTGTGGAG GTATGTCCGT 
GCAAGCATGT CCTTGTCAGG CTATATGCCC CCACTCTGCG ACCCCAAGGA TGGACACCTG 
CTGATGGATG GTGGTTACAT CAACAACCTC CCAGCGGATG TGGCAAGATC CATGGGGGCA 
AAGGTTGTGA TTGCCATCGA TGTTGGCAGC CGGGACGAGA CAGACCTCAC CAACTATGGC 
GATGCCTTGT CTGGATGGTG GCTGCTTTGG AAACGCTGGA ACCCCTTGGC TACAAAAGTC 
AAGGTGTTGA ACATGGCAGA GATTCAGACT CGACTGGCCT ATGTGTGCTG CGTCCGGCAG 
CTGGAGATGG TGAAGAACAG CGACTACTGT GAGTACCTGA GGCCTCCCAT CGACAGTTAC 
CGCACCCTGG ACTTCGGCAA ATTTGATGAG ATCTGTGAAG TTGGGTATCA ACATGGGCGG 
ACAGTGTTTG ACATCTGGGT CCGGAGTGGT GTCCTAGAGA AGATGTTGCA GGACCAGCAG 
GGGACAAGCA AGAGGAAGGA CTGTGGGGTG TTCACCTGCC CCAATTCCTC CTTCACGGAC 
CTTGCTGAGA TTGTGTCACG GATTGAGCCG GCCAAGGTGG CTGCAGTGGA TGATGAGTCT 
GACTATCAGA CCGAGTATGA GGAGGAACTG CCAGCCATCC CCAAGGAAAC GTATGCTGAC 
TTCCAGAGCA CTGGGATTGA GCTAGACTCT GACTCGGAAT ATGAGCCTTC GATGTTGCAA 
GGACCCCCCA GCTTGACTTC CCCAGAACAG TCCCAGGACT CCTTCCCTTG GCTCCCTAAT 
CAAGATGACC AGGGGCCAAG ACTGGAACAT CCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666363.3
CDS	252..4310
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDADGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	NM_146251.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146251.4

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGATG GCACCGAAGT GGTGGTCAAA GAGGTCCTGC CAGGGGACAG TGTCCACAGT 
CTCCTCAGCA TTTTGGATGT GATCACCGGT CACACTGCAC CATACAAAAC AGTTTCTGCC 
CGTGCCGCTG TCTCGTCCAC CGTGCTCTGG CTTCCTGCAG CAGCTTTTCA AGGAGTGTTT 
GAGAAATACC CTGAAACTCT GGTCAGGGTT GTGCAGATCA TCATGGTCCG GCTGCAGCGG 
GTGACTTTCC TGGCTCTGCA CAACTACCTG GGGCTGACTA CAGAGCTCTT CAACCCTGAG 
AGCCAGGCCA TCCCTCTCTT GTCAGTAGCA AGTGTGGCTG GACGAGCCAA GAGACAGATG 
TCATATGGTC CAGAGGAGCA GCTTGAGAGG TCCCTACGGC CCTCGGAGTT CAGCAGCTCA 
GATCATGGGA GCAGCTGTGT GACAGTCTCT GGGCCTCTGC TGAAGAGGAG CTGCTCAGTG 
CCTCTGCCTT CTAACCATGG GGAGGTGGAT GAGCTCAGAC AGTCCCAGGG AAGTGGCTCT 
AACACTTCTG CCTTCCAAGA AAGCCATGAG GGAGCCACCT CAGACCTGGG GATGGCATAC 
AACCGTGCCA GGATTTTGCC ACACTCTGAT GAGCAACTTG GGAACTCTTT GGCCAGCAAG 
TCCAAGAAAA GTGTTGTCGC AGAGACCCCC TCTGCCATCT TCCACTACTC AGAAAATTTC 
AGGGATGAGA CTGGGGCCTG TGGGAAGACA GATGCTATCT TCAGAGCTGC CACAAAGGAC 
CTCCTTACCC TGATGAAGCT AGATGACCCA TCCCTGTTGG ATGGCCGGGT GGCATTTCTT 
CATGTTCCTG CAGGCACCTT AGTGTCAAAA CAGGGAGATC AGGATGTCAA TATTTTGTTT 
GTGGTCTCGG GGATGCTGCA TGTGTACCAG CAAAAGATTG ACAGCTTGGA GGATACTTGC 
CTGTTCCTCA CACATCCCGG GGAGATGGTG GGTCAGCTGG CGGTGCTCAC TGGAGAACCC 
CTAATGTTCA CCATCAGAGC CAACCGAGAC TGCAGCTTTT TGTCCATCTC GAAGGCCCAC 
TTCTATGAGA TCATGAGGAA ACGGCCGGAT GTTGTCCTGG GCGTGGCACA CACAGTTGTG 
AAGAGGATGT CATCCTTTGT ACGGCAGATT GATTTTGCTC TAGACTGGAT GGAAGTAGAG 
GCTGGACGTG CCATATACAG GCAAGGAGAC AAGTCTGACT GCACATACAT CGTTCTCAGT 
GGCAGGCTGC GTTCTGTCAT CCGAAAAGAT GATGGGAAAA AACGCCTGGC AGGGGAATAT 
GGCCGAGGAG ACCTTGTTGG TGTGGTGGAG ACGCTGACCC ACCAGGCCAG AGCAACCACA 
GTGCATGCTG TCCGGGACTC AGAACTGGCT AAGCTGCCAG CAGGAGCTCT GACGTCTATC 
AAGCGCAGGT ACCCACAGGT TGTAACGCGA CTGATTCATC TCTTGGGCGA GAAGATCTTA 
GGCAGTCTCC AGCAGGGATC TGCAACAGGT CACCAGCTTG GATTTAACAC AGCAAGCAGC 
AAATGGGACT TGGGGAACCC GCCTGGCAAT CTGTCCACAG TGGCAGCACT GCCTGCTTCT 
GAGGATGTGC CCCTAACCGC CTTTGCTCTG GAGCTCCAAC ATGCCCTCAG TGCCATCGGT 
CCTGTCCTGC TGCTGACCAG TGACAACATC AAACAACGCC TGGGTTCCGC TGCTCTAGAC 
AGCATCCATG AGTACCGGCT CTCCAGTTGG CTGGGCCAGC AGGAGGACAT CCACCGGATT 
GTGCTGTATC AGGCAGACGG CACACTCACA CCGTGGACCC AGCGCTGCAT CCGGCAGGCT 
GACTGCATCC TCATCGTGGG CTTGGGTGAG CAAGAGCCAG CAGTAGGTGA GCTGGAGCAG 
ATGTTGGAGA GCACAGCTGT GCGTGCCCAG AAGCAGCTAA TCTTGCTGCA CAAAGAGGAC 
GGTCCAGTGC CTTCCCGCAC AGTGGAGTGG CTCAACATGC GAAGTTGGTG TTCTGGCCAT 
TTGCACCTCT GCTGCCCACG CCGTGTCTTC TCCAAGAGAA GCTTGCCCAA GCTGGTCGAG 
ATGTACACAC GTGTTTTCCA ACGACCACCA GACCGTCATT CTGATTTTTC CCGCCTGGCA 
CGGATGCTGA CAGGCAATGC CATTGCCTTG GTGCTTGGAG GGGGTGGAGC TAGAGGGTGT 
GCCCAGGTGG GCATCCTCAG GGCACTAGCA GAGTGTGGCG TCCCCGTGGA CATAATCGGA 
GGCACATCCA TCGGGGCTTT CATGGGTGCT CTGTTTGCTG AGGAGCGGAG CTACAGCCAA 
ACACGGATCC GGGCCAAGCA GTGGGCAGAG GGTATGACAT CAATGATGAA GACTATACTG 
GACCTGACCT ACCCAATCAC ATCCATGTTC TCTGGCACTG GCTTCAATAG CAGCATCAGC 
AACATCTTCA AAGACCGGCA GATTGAGGAC CTGTGGCTCC CCTACTTTGC CATCACCACC 
GACATCACGG CCTCTGCCAT GCGGGTTCAC ACAGATGGCT CTCTGTGGAG GTATGTCCGT 
GCAAGCATGT CCTTGTCAGG CTATATGCCC CCACTCTGCG ACCCCAAGGA TGGACACCTG 
CTGATGGATG GTGGTTACAT CAACAACCTC CCAGCGGATG TGGCAAGATC CATGGGGGCA 
AAGGTTGTGA TTGCCATCGA TGTTGGCAGC CGGGACGAGA CAGACCTCAC CAACTATGGC 
GATGCCTTGT CTGGATGGTG GCTGCTTTGG AAACGCTGGA ACCCCTTGGC TACAAAAGTC 
AAGGTGTTGA ACATGGCAGA GATTCAGACT CGACTGGCCT ATGTGTGCTG CGTCCGGCAG 
CTGGAGATGG TGAAGAACAG CGACTACTGT GAGTACCTGA GGCCTCCCAT CGACAGTTAC 
CGCACCCTGG ACTTCGGCAA ATTTGATGAG ATCTGTGAAG TTGGGTATCA ACATGGGCGG 
ACAGTGTTTG ACATCTGGGT CCGGAGTGGT GTCCTAGAGA AGATGTTGCA GGACCAGCAG 
GGGACAAGCA AGAGGAAGGA CTGTGGGGTG TTCACCTGCC CCAATTCCTC CTTCACGGAC 
CTTGCTGAGA TTGTGTCACG GATTGAGCCG GCCAAGGTGG CTGCAGTGGA TGATGAGTCT 
GACTATCAGA CCGAGTATGA GGAGGAACTG CCAGCCATCC CCAAGGAAAC GTATGCTGAC 
TTCCAGAGCA CTGGGATTGA GCTAGACTCT GACTCGGAAT ATGAGCCTTC GATGTTGCAA 
GGACCCCCCA GCTTGACTTC CCCAGAACAG TCCCAGGACT CCTTCCCTTG GCTCCCTAAT 
CAAGATGACC AGGGGCCAAG ACTGGAACAT CCTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498015.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498078.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X3, mRNA.

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CloneID	OMu29538
Clone ID Related Accession (Same CDS sequence)	XM_006498015.3 , XM_006498015.4
Accession Version	XM_006498015.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498015.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGATG GCACCGAAGT GGTGGTCAAA GAGGTCCTGC CAGGGGACAG TGTCCACAGT 
CTCCTCAGCA TTTTGGATGT GATCACCGGT CACACTGCAC CATACAAAAC AGTTTCTGCC 
CGTGCCGCTG TCTCGTCCAC CGTGCTCTGG CTTCCTGCAG CAGCTTTTCA AGGAGTGTTT 
GAGAAATACC CTGAAACTCT GGTCAGGGTT GTGCAGATCA TCATGGTCCG GCTGCAGCGG 
GTGACTTTCC TGGCTCTGCA CAACTACCTG GGGCTGACTA CAGAGCTCTT CAACCCTGAG 
AGCCAGGCCA TCCCTCTCTT GTCAGTAGCA AGTGTGGCTG GACGAGCCAA GAGACAGATG 
TCATATGGTC CAGAGGAGCA GCTTGAGAGG TCCCTACGGC CCTCGGAGTT CAGCAGCTCA 
GATCATGGGA GCAGCTGTGT GACAGTCTCT GGGCCTCTGC TGAAGAGGAG CTGCTCAGTG 
CCTCTGCCTT CTAACCATGG GGAGGTGGAT GAGCTCAGAC AGTCCCAGGG AAGTGGCTCT 
AACACTTCTG CCTTCCAAGA AAGCCATGAG GGAGCCACCT CAGACCTGGG GATGGCATAC 
AACCGTGCCA GGATTTTGCC ACACTCTGAT GAGCAACTTG GGAACTCTTT GGCCAGCAAG 
TCCAAGAAAA GTGTTGTCGC AGAGACCCCC TCTGCCATCT TCCACTACTC AGAAAATTTC 
AGGGATGAGA CTGGGGCCTG TGGGAAGACA GATGCTATCT TCAGAGCTGC CACAAAGGAC 
CTCCTTACCC TGATGAAGCT AGATGACCCA TCCCTGTTGG ATGGCCGGGT GGCATTTCTT 
CATGTTCCTG CAGGCACCTT AGTGTCAAAA CAGGGAGATC AGGATGTCAA TATTTTGTTT 
GTGGTCTCGG GGATGCTGCA TGTGTACCAG CAAAAGATTG ACAGCTTGGA GGATACTTGC 
CTGTTCCTCA CACATCCCGG GGAGATGGTG GGTCAGCTGG CGGTGCTCAC TGGAGAACCC 
CTAATGTTCA CCATCAGAGC CAACCGAGAC TGCAGCTTTT TGTCCATCTC GAAGGCCCAC 
TTCTATGAGA TCATGAGGAA ACGGCCGGAT GTTGTCCTGG GCGTGGCACA CACAGTTGTG 
AAGAGGATGT CATCCTTTGT ACGGCAGATT GATTTTGCTC TAGACTGGAT GGAAGTAGAG 
GCTGGACGTG CCATATACAG GCAAGGAGAC AAGTCTGACT GCACATACAT CGTTCTCAGT 
GGCAGGCTGC GTTCTGTCAT CCGAAAAGAT GATGGGAAAA AACGCCTGGC AGGGGAATAT 
GGCCGAGGAG ACCTTGTTGG TGTGGTGGAG ACGCTGACCC ACCAGGCCAG AGCAACCACA 
GTGCATGCTG TCCGGGACTC AGAACTGGCT AAGCTGCCAG CAGGAGCTCT GACGTCTATC 
AAGCGCAGGT ACCCACAGGT TGTAACGCGA CTGATTCATC TCTTGGGCGA GAAGATCTTA 
GGCAGTCTCC AGCAGGGATC TGCAACAGGT CACCAGCTTG GATTTAACAC AGCAAGCAGC 
AAATGGGACT TGGGGAACCC GCCTGGCAAT CTGTCCACAG TGGCAGCACT GCCTGCTTCT 
GAGGATGTGC CCCTAACCGC CTTTGCTCTG GAGCTCCAAC ATGCCCTCAG TGCCATCGGT 
CCTGTCCTGC TGCTGACCAG TGACAACATC AAACAACGCC TGGGTTCCGC TGCTCTAGAC 
AGCATCCATG AGTACCGGCT CTCCAGTTGG CTGGGCCAGC AGGAGGACAT CCACCGGATT 
GTGCTGTATC AGGCAGACGG CACACTCACA CCGTGGACCC AGCGCTGCAT CCGGCAGGCT 
GACTGCATCC TCATCGTGGG CTTGGGTGAG CAAGAGCCAG CAGTAGGTGA GCTGGAGCAG 
ATGTTGGAGA GCACAGCTGT GCGTGCCCAG AAGCAGCTAA TCTTGCTGCA CAAAGAGGAC 
GGTCCAGTGC CTTCCCGCAC AGTGGAGTGG CTCAACATGC GAAGTTGGTG TTCTGGCCAT 
TTGCACCTCT GCTGCCCACG CCGTGTCTTC TCCAAGAGAA GCTTGCCCAA GCTGGTCGAG 
ATGTACACAC GTGTTTTCCA ACGACCACCA GACCGTCATT CTGATTTTTC CCGCCTGGCA 
CGGATGCTGA CAGGCAATGC CATTGCCTTG GTGCTTGGAG GGGGTGGAGC TAGAGGGTGT 
GCCCAGGTGG GCATCCTCAG GGCACTAGCA GAGTGTGGCG TCCCCGTGGA CATAATCGGA 
GGCACATCCA TCGGGGCTTT CATGGGTGCT CTGTTTGCTG AGGAGCGGAG CTACAGCCAA 
ACACGGATCC GGGCCAAGCA GTGGGCAGAG GGTATGACAT CAATGATGAA GACTATACTG 
GACCTGACCT ACCCAATCAC ATCCATGTTC TCTGGCACTG GCTTCAATAG CAGCATCAGC 
AACATCTTCA AAGACCGGCA GATTGAGGAC CTGTGGCTCC CCTACTTTGC CATCACCACC 
GACATCACGG CCTCTGCCAT GCGGGTTCAC ACAGATGGCT CTCTGTGGAG GTATGTCCGT 
GCAAGCATGT CCTTGTCAGG CTATATGCCC CCACTCTGCG ACCCCAAGGA TGGACACCTG 
CTGATGGATG GTGGTTACAT CAACAACCTC CCAGCGGATG TGGCAAGATC CATGGGGGCA 
AAGGTTGTGA TTGCCATCGA TGTTGGCAGC CGGGACGAGA CAGACCTCAC CAACTATGGC 
GATGCCTTGT CTGGATGGTG GCTGCTTTGG AAACGCTGGA ACCCCTTGGC TACAAAAGTC 
AAGGTGTTGA ACATGGCAGA GATTCAGACT CGACTGGCCT ATGTGTGCTG CGTCCGGCAG 
CTGGAGATGG TGAAGAACAG CGACTACTGT GAGTACCTGA GGCCTCCCAT CGACAGTTAC 
CGCACCCTGG ACTTCGGCAA ATTTGATGAG ATCTGTGAAG TTGGGTATCA ACATGGGCGG 
ACAGTGTTTG ACATCTGGGT CCGGAGTGGT GTCCTAGAGA AGATGTTGCA GGACCAGCAG 
GGGACAAGCA AGAGGAAGGA CTGTGGGGTG TTCACCTGCC CCAATTCCTC CTTCACGGAC 
CTTGCTGAGA TTGTGTCACG GATTGAGCCG GCCAAGGTGG CTGCAGTGGA TGCCTTGCTT 
GGCACAGCCA CTGTCCTTGG AGAGAGGACC AATGGAGCCC TCTCTTCTGC TCTAGATGAG 
TCTGACTATC AGACCGAGTA TGAGGAGGAA CTGCCAGCCA TCCCCAAGGA AACGTATGCT 
GACTTCCAGA GCACTGGGAT TGAGCTAGAC TCTGACTCGG AATATGAGCC TTCGATGTTG 
CAAGGACCCC CCAGCTTGAC TTCCCCAGAA CAGTCCCAGG ACTCCTTCCC TTGGCTCCCT 
AATCAAGATG ACCAGGGGCC AAGACTGGAA CATCCTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498078.1
CDS	976..5097
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDADGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498015.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498015.3

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGATG GCACCGAAGT GGTGGTCAAA GAGGTCCTGC CAGGGGACAG TGTCCACAGT 
CTCCTCAGCA TTTTGGATGT GATCACCGGT CACACTGCAC CATACAAAAC AGTTTCTGCC 
CGTGCCGCTG TCTCGTCCAC CGTGCTCTGG CTTCCTGCAG CAGCTTTTCA AGGAGTGTTT 
GAGAAATACC CTGAAACTCT GGTCAGGGTT GTGCAGATCA TCATGGTCCG GCTGCAGCGG 
GTGACTTTCC TGGCTCTGCA CAACTACCTG GGGCTGACTA CAGAGCTCTT CAACCCTGAG 
AGCCAGGCCA TCCCTCTCTT GTCAGTAGCA AGTGTGGCTG GACGAGCCAA GAGACAGATG 
TCATATGGTC CAGAGGAGCA GCTTGAGAGG TCCCTACGGC CCTCGGAGTT CAGCAGCTCA 
GATCATGGGA GCAGCTGTGT GACAGTCTCT GGGCCTCTGC TGAAGAGGAG CTGCTCAGTG 
CCTCTGCCTT CTAACCATGG GGAGGTGGAT GAGCTCAGAC AGTCCCAGGG AAGTGGCTCT 
AACACTTCTG CCTTCCAAGA AAGCCATGAG GGAGCCACCT CAGACCTGGG GATGGCATAC 
AACCGTGCCA GGATTTTGCC ACACTCTGAT GAGCAACTTG GGAACTCTTT GGCCAGCAAG 
TCCAAGAAAA GTGTTGTCGC AGAGACCCCC TCTGCCATCT TCCACTACTC AGAAAATTTC 
AGGGATGAGA CTGGGGCCTG TGGGAAGACA GATGCTATCT TCAGAGCTGC CACAAAGGAC 
CTCCTTACCC TGATGAAGCT AGATGACCCA TCCCTGTTGG ATGGCCGGGT GGCATTTCTT 
CATGTTCCTG CAGGCACCTT AGTGTCAAAA CAGGGAGATC AGGATGTCAA TATTTTGTTT 
GTGGTCTCGG GGATGCTGCA TGTGTACCAG CAAAAGATTG ACAGCTTGGA GGATACTTGC 
CTGTTCCTCA CACATCCCGG GGAGATGGTG GGTCAGCTGG CGGTGCTCAC TGGAGAACCC 
CTAATGTTCA CCATCAGAGC CAACCGAGAC TGCAGCTTTT TGTCCATCTC GAAGGCCCAC 
TTCTATGAGA TCATGAGGAA ACGGCCGGAT GTTGTCCTGG GCGTGGCACA CACAGTTGTG 
AAGAGGATGT CATCCTTTGT ACGGCAGATT GATTTTGCTC TAGACTGGAT GGAAGTAGAG 
GCTGGACGTG CCATATACAG GCAAGGAGAC AAGTCTGACT GCACATACAT CGTTCTCAGT 
GGCAGGCTGC GTTCTGTCAT CCGAAAAGAT GATGGGAAAA AACGCCTGGC AGGGGAATAT 
GGCCGAGGAG ACCTTGTTGG TGTGGTGGAG ACGCTGACCC ACCAGGCCAG AGCAACCACA 
GTGCATGCTG TCCGGGACTC AGAACTGGCT AAGCTGCCAG CAGGAGCTCT GACGTCTATC 
AAGCGCAGGT ACCCACAGGT TGTAACGCGA CTGATTCATC TCTTGGGCGA GAAGATCTTA 
GGCAGTCTCC AGCAGGGATC TGCAACAGGT CACCAGCTTG GATTTAACAC AGCAAGCAGC 
AAATGGGACT TGGGGAACCC GCCTGGCAAT CTGTCCACAG TGGCAGCACT GCCTGCTTCT 
GAGGATGTGC CCCTAACCGC CTTTGCTCTG GAGCTCCAAC ATGCCCTCAG TGCCATCGGT 
CCTGTCCTGC TGCTGACCAG TGACAACATC AAACAACGCC TGGGTTCCGC TGCTCTAGAC 
AGCATCCATG AGTACCGGCT CTCCAGTTGG CTGGGCCAGC AGGAGGACAT CCACCGGATT 
GTGCTGTATC AGGCAGACGG CACACTCACA CCGTGGACCC AGCGCTGCAT CCGGCAGGCT 
GACTGCATCC TCATCGTGGG CTTGGGTGAG CAAGAGCCAG CAGTAGGTGA GCTGGAGCAG 
ATGTTGGAGA GCACAGCTGT GCGTGCCCAG AAGCAGCTAA TCTTGCTGCA CAAAGAGGAC 
GGTCCAGTGC CTTCCCGCAC AGTGGAGTGG CTCAACATGC GAAGTTGGTG TTCTGGCCAT 
TTGCACCTCT GCTGCCCACG CCGTGTCTTC TCCAAGAGAA GCTTGCCCAA GCTGGTCGAG 
ATGTACACAC GTGTTTTCCA ACGACCACCA GACCGTCATT CTGATTTTTC CCGCCTGGCA 
CGGATGCTGA CAGGCAATGC CATTGCCTTG GTGCTTGGAG GGGGTGGAGC TAGAGGGTGT 
GCCCAGGTGG GCATCCTCAG GGCACTAGCA GAGTGTGGCG TCCCCGTGGA CATAATCGGA 
GGCACATCCA TCGGGGCTTT CATGGGTGCT CTGTTTGCTG AGGAGCGGAG CTACAGCCAA 
ACACGGATCC GGGCCAAGCA GTGGGCAGAG GGTATGACAT CAATGATGAA GACTATACTG 
GACCTGACCT ACCCAATCAC ATCCATGTTC TCTGGCACTG GCTTCAATAG CAGCATCAGC 
AACATCTTCA AAGACCGGCA GATTGAGGAC CTGTGGCTCC CCTACTTTGC CATCACCACC 
GACATCACGG CCTCTGCCAT GCGGGTTCAC ACAGATGGCT CTCTGTGGAG GTATGTCCGT 
GCAAGCATGT CCTTGTCAGG CTATATGCCC CCACTCTGCG ACCCCAAGGA TGGACACCTG 
CTGATGGATG GTGGTTACAT CAACAACCTC CCAGCGGATG TGGCAAGATC CATGGGGGCA 
AAGGTTGTGA TTGCCATCGA TGTTGGCAGC CGGGACGAGA CAGACCTCAC CAACTATGGC 
GATGCCTTGT CTGGATGGTG GCTGCTTTGG AAACGCTGGA ACCCCTTGGC TACAAAAGTC 
AAGGTGTTGA ACATGGCAGA GATTCAGACT CGACTGGCCT ATGTGTGCTG CGTCCGGCAG 
CTGGAGATGG TGAAGAACAG CGACTACTGT GAGTACCTGA GGCCTCCCAT CGACAGTTAC 
CGCACCCTGG ACTTCGGCAA ATTTGATGAG ATCTGTGAAG TTGGGTATCA ACATGGGCGG 
ACAGTGTTTG ACATCTGGGT CCGGAGTGGT GTCCTAGAGA AGATGTTGCA GGACCAGCAG 
GGGACAAGCA AGAGGAAGGA CTGTGGGGTG TTCACCTGCC CCAATTCCTC CTTCACGGAC 
CTTGCTGAGA TTGTGTCACG GATTGAGCCG GCCAAGGTGG CTGCAGTGGA TGCCTTGCTT 
GGCACAGCCA CTGTCCTTGG AGAGAGGACC AATGGAGCCC TCTCTTCTGC TCTAGATGAG 
TCTGACTATC AGACCGAGTA TGAGGAGGAA CTGCCAGCCA TCCCCAAGGA AACGTATGCT 
GACTTCCAGA GCACTGGGAT TGAGCTAGAC TCTGACTCGG AATATGAGCC TTCGATGTTG 
CAAGGACCCC CCAGCTTGAC TTCCCCAGAA CAGTCCCAGG ACTCCTTCCC TTGGCTCCCT 
AATCAAGATG ACCAGGGGCC AAGACTGGAA CATCCTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498016.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498079.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X4, mRNA.

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CloneID	OMu29539
Clone ID Related Accession (Same CDS sequence)	XM_006498016.4 , XM_006498016.3
Accession Version	XM_006498016.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498016.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGAATA TGAGCCTTCG 
ATGTTGCAAG GACCCCCCAG CTTGACTTCC CCAGAACAGT CCCAGGACTC CTTCCCTTGG 
CTCCCTAATC AAGATGACCA GGGGCCAAGA CTGGAACATC CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498079.1
CDS	976..5043
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498016.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498016.3

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GATGAGTCTG ACTATCAGAC CGAGTATGAG GAGGAACTGC CAGCCATCCC CAAGGAAACG 
TATGCTGACT TCCAGAGCAC TGGGATTGAG CTAGACTCTG ACTCGGAATA TGAGCCTTCG 
ATGTTGCAAG GACCCCCCAG CTTGACTTCC CCAGAACAGT CCCAGGACTC CTTCCCTTGG 
CTCCCTAATC AAGATGACCA GGGGCCAAGA CTGGAACATC CTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498019.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498082.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu29540
Clone ID Related Accession (Same CDS sequence)	XM_006498018.4 , XM_006498019.3 , XM_006498017.4 , XM_006498019.4 , XM_006498018.3 , XM_006498017.3
Accession Version	XM_006498019.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498019.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498082.1
CDS	958..5010
Translation	MEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498019.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498019.3

ORF Insert Sequence:

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498018.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498081.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X6, mRNA.

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CloneID	OMu29540
Clone ID Related Accession (Same CDS sequence)	XM_006498018.4 , XM_006498019.3 , XM_006498017.4 , XM_006498019.4 , XM_006498018.3 , XM_006498017.3
Accession Version	XM_006498018.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498018.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498081.1
CDS	1054..5106
Translation	MEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498018.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498018.3

ORF Insert Sequence:

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498017.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498080.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X5, mRNA.

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CloneID	OMu29540
Clone ID Related Accession (Same CDS sequence)	XM_006498018.4 , XM_006498019.3 , XM_006498017.4 , XM_006498019.4 , XM_006498018.3 , XM_006498017.3
Accession Version	XM_006498017.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498080.1
CDS	1150..5202
Translation	MEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_006498017.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498017.3

ORF Insert Sequence:

ATGGAGGAGC AGTCCCAGTC CACCATGCTG ATGGGGATTG GAATTGGCGC CCTCCTTACC 
CTAGCCTTCG TTGGTATTAC CTTCTTTTTT GTATACAGAA GAGTGAGAAG ATTACGACGG 
GCAGAACCCA CTCCTCAGTA CAGGTTCCGG AAGAGAGACA AAGTAATGTT TTATGGCCGG 
AAGATCATGA GGAAGGTGAC CACACTCCCC CATACCTTGG TTGGGAATAC CTCTGCACCC 
CGGCAGCGAG TCAGGAAGAG GACCAAAGTG CTGTCTTTAG CCAAGAGGAT TCTGCGCTTT 
AAGAAAGAGT ATCCCACTTT GCAGCCAAAA GAGCCTCCGC CCTCCCTGCT TGAGGCTGAT 
CTTACAGAGT TTGATGTGAA GAACTCTCAT CTGCCATCAG AAGTCTTGTA CATGTTGAAA 
AATGTTCGGG TTCTAGGCCA CTTTGAGAAG CCACTATTTC TGGAACTTTG CAAACATATG 
GTCTTTGTGC AGCTACAAGA AGGGGAGCAC GTATTCCAAC CTGGGGAGCC TGACATCAGC 
ATCTATGTGG TTCAGGACGG ACGGCTGGAG GTCTGCATCC AAGACGCTGT GACACAGGAT 
GGCACCGAAG TGGTGGTCAA AGAGGTCCTG CCAGGGGACA GTGTCCACAG TCTCCTCAGC 
ATTTTGGATG TGATCACCGG TCACACTGCA CCATACAAAA CAGTTTCTGC CCGTGCCGCT 
GTCTCGTCCA CCGTGCTCTG GCTTCCTGCA GCAGCTTTTC AAGGAGTGTT TGAGAAATAC 
CCTGAAACTC TGGTCAGGGT TGTGCAGATC ATCATGGTCC GGCTGCAGCG GGTGACTTTC 
CTGGCTCTGC ACAACTACCT GGGGCTGACT ACAGAGCTCT TCAACCCTGA GAGCCAGGCC 
ATCCCTCTCT TGTCAGTAGC AAGTGTGGCT GGACGAGCCA AGAGACAGAT GTCATATGGT 
CCAGAGGAGC AGCTTGAGAG GTCCCTACGG CCCTCGGAGT TCAGCAGCTC AGATCATGGG 
AGCAGCTGTG TGACAGTCTC TGGGCCTCTG CTGAAGAGGA GCTGCTCAGT GCCTCTGCCT 
TCTAACCATG GGGAGGTGGA TGAGCTCAGA CAGTCCCAGG GAAGTGGCTC TAACACTTCT 
GCCTTCCAAG AAAGCCATGA GGGAGCCACC TCAGACCTGG GGATGGCATA CAACCGTGCC 
AGGATTTTGC CACACTCTGA TGAGCAACTT GGGAACTCTT TGGCCAGCAA GTCCAAGAAA 
AGTGTTGTCG CAGAGACCCC CTCTGCCATC TTCCACTACT CAGAAAATTT CAGGGATGAG 
ACTGGGGCCT GTGGGAAGAC AGATGCTATC TTCAGAGCTG CCACAAAGGA CCTCCTTACC 
CTGATGAAGC TAGATGACCC ATCCCTGTTG GATGGCCGGG TGGCATTTCT TCATGTTCCT 
GCAGGCACCT TAGTGTCAAA ACAGGGAGAT CAGGATGTCA ATATTTTGTT TGTGGTCTCG 
GGGATGCTGC ATGTGTACCA GCAAAAGATT GACAGCTTGG AGGATACTTG CCTGTTCCTC 
ACACATCCCG GGGAGATGGT GGGTCAGCTG GCGGTGCTCA CTGGAGAACC CCTAATGTTC 
ACCATCAGAG CCAACCGAGA CTGCAGCTTT TTGTCCATCT CGAAGGCCCA CTTCTATGAG 
ATCATGAGGA AACGGCCGGA TGTTGTCCTG GGCGTGGCAC ACACAGTTGT GAAGAGGATG 
TCATCCTTTG TACGGCAGAT TGATTTTGCT CTAGACTGGA TGGAAGTAGA GGCTGGACGT 
GCCATATACA GGCAAGGAGA CAAGTCTGAC TGCACATACA TCGTTCTCAG TGGCAGGCTG 
CGTTCTGTCA TCCGAAAAGA TGATGGGAAA AAACGCCTGG CAGGGGAATA TGGCCGAGGA 
GACCTTGTTG GTGTGGTGGA GACGCTGACC CACCAGGCCA GAGCAACCAC AGTGCATGCT 
GTCCGGGACT CAGAACTGGC TAAGCTGCCA GCAGGAGCTC TGACGTCTAT CAAGCGCAGG 
TACCCACAGG TTGTAACGCG ACTGATTCAT CTCTTGGGCG AGAAGATCTT AGGCAGTCTC 
CAGCAGGGAT CTGCAACAGG TCACCAGCTT GGATTTAACA CAGCAAGCAG CAAATGGGAC 
TTGGGGAACC CGCCTGGCAA TCTGTCCACA GTGGCAGCAC TGCCTGCTTC TGAGGATGTG 
CCCCTAACCG CCTTTGCTCT GGAGCTCCAA CATGCCCTCA GTGCCATCGG TCCTGTCCTG 
CTGCTGACCA GTGACAACAT CAAACAACGC CTGGGTTCCG CTGCTCTAGA CAGCATCCAT 
GAGTACCGGC TCTCCAGTTG GCTGGGCCAG CAGGAGGACA TCCACCGGAT TGTGCTGTAT 
CAGGCAGACG GCACACTCAC ACCGTGGACC CAGCGCTGCA TCCGGCAGGC TGACTGCATC 
CTCATCGTGG GCTTGGGTGA GCAAGAGCCA GCAGTAGGTG AGCTGGAGCA GATGTTGGAG 
AGCACAGCTG TGCGTGCCCA GAAGCAGCTA ATCTTGCTGC ACAAAGAGGA CGGTCCAGTG 
CCTTCCCGCA CAGTGGAGTG GCTCAACATG CGAAGTTGGT GTTCTGGCCA TTTGCACCTC 
TGCTGCCCAC GCCGTGTCTT CTCCAAGAGA AGCTTGCCCA AGCTGGTCGA GATGTACACA 
CGTGTTTTCC AACGACCACC AGACCGTCAT TCTGATTTTT CCCGCCTGGC ACGGATGCTG 
ACAGGCAATG CCATTGCCTT GGTGCTTGGA GGGGGTGGAG CTAGAGGGTG TGCCCAGGTG 
GGCATCCTCA GGGCACTAGC AGAGTGTGGC GTCCCCGTGG ACATAATCGG AGGCACATCC 
ATCGGGGCTT TCATGGGTGC TCTGTTTGCT GAGGAGCGGA GCTACAGCCA AACACGGATC 
CGGGCCAAGC AGTGGGCAGA GGGTATGACA TCAATGATGA AGACTATACT GGACCTGACC 
TACCCAATCA CATCCATGTT CTCTGGCACT GGCTTCAATA GCAGCATCAG CAACATCTTC 
AAAGACCGGC AGATTGAGGA CCTGTGGCTC CCCTACTTTG CCATCACCAC CGACATCACG 
GCCTCTGCCA TGCGGGTTCA CACAGATGGC TCTCTGTGGA GGTATGTCCG TGCAAGCATG 
TCCTTGTCAG GCTATATGCC CCCACTCTGC GACCCCAAGG ATGGACACCT GCTGATGGAT 
GGTGGTTACA TCAACAACCT CCCAGCGGAT GTGGCAAGAT CCATGGGGGC AAAGGTTGTG 
ATTGCCATCG ATGTTGGCAG CCGGGACGAG ACAGACCTCA CCAACTATGG CGATGCCTTG 
TCTGGATGGT GGCTGCTTTG GAAACGCTGG AACCCCTTGG CTACAAAAGT CAAGGTGTTG 
AACATGGCAG AGATTCAGAC TCGACTGGCC TATGTGTGCT GCGTCCGGCA GCTGGAGATG 
GTGAAGAACA GCGACTACTG TGAGTACCTG AGGCCTCCCA TCGACAGTTA CCGCACCCTG 
GACTTCGGCA AATTTGATGA GATCTGTGAA GTTGGGTATC AACATGGGCG GACAGTGTTT 
GACATCTGGG TCCGGAGTGG TGTCCTAGAG AAGATGTTGC AGGACCAGCA GGGGACAAGC 
AAGAGGAAGG ACTGTGGGGT GTTCACCTGC CCCAATTCCT CCTTCACGGA CCTTGCTGAG 
ATTGTGTCAC GGATTGAGCC GGCCAAGGTG GCTGCAGTGG ATGCCTTGCT TGGCACAGCC 
ACTGTCCTTG GAGAGAGGAC CAATGGAGCC CTCTCTTCTG CTCTAGATGA GTCTGACTAT 
CAGACCGAGT ATGAGGAGGA ACTGCCAGCC ATCCCCAAGG AAACGTATGC TGACTTCCAG 
AGCACTGGGA TTGAGCTAGA CTCTGACTCG GAATATGAGC CTTCGATGTT GCAAGGACCC 
CCCAGCTTGA CTTCCCCAGA ACAGTCCCAG GACTCCTTCC CTTGGCTCCC TAATCAAGAT 
GACCAGGGGC CAAGACTGGA ACATCCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_006498020.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498083.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu29541
Clone ID Related Accession (Same CDS sequence)	XM_006498020.4 , XM_006498020.3
Accession Version	XM_006498020.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498020.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GCCTTGCTTG GCACAGCCAC TGTCCTTGGA GAGAGGACCA ATGGAGCCCT CTCTTCTGCT 
CTAGATGAGT CTGACTATCA GACCGAGTAT GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA 
ACGTATGCTG ACTTCCAGAG CACTGGGATT GAGCTAGACT CTGACTCGGT GAGGAATATG 
AGCCTTCGAT GTTGCAAGGA CCCCCCAGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498083.1
CDS	977..5029
Translation	MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSVRNMSLRCCKDPPA

Target ORF information:

RefSeq Version	XM_006498020.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498020.3

ORF Insert Sequence:

ATGCAGAATG AGGAAGATGC CTGCCTTGAG GCTGGCTACT GTTTGGGTAC CACCCTGAGC 
TCCTGGAGGC TGCATTTCAT GGAGGAGCAG TCCCAGTCCA CCATGCTGAT GGGGATTGGA 
ATTGGCGCCC TCCTTACCCT AGCCTTCGTT GGTATTACCT TCTTTTTTGT ATACAGAAGA 
GTGAGAAGAT TACGACGGGC AGAACCCACT CCTCAGTACA GGTTCCGGAA GAGAGACAAA 
GTAATGTTTT ATGGCCGGAA GATCATGAGG AAGGTGACCA CACTCCCCCA TACCTTGGTT 
GGGAATACCT CTGCACCCCG GCAGCGAGTC AGGAAGAGGA CCAAAGTGCT GTCTTTAGCC 
AAGAGGATTC TGCGCTTTAA GAAAGAGTAT CCCACTTTGC AGCCAAAAGA GCCTCCGCCC 
TCCCTGCTTG AGGCTGATCT TACAGAGTTT GATGTGAAGA ACTCTCATCT GCCATCAGAA 
GTCTTGTACA TGTTGAAAAA TGTTCGGGTT CTAGGCCACT TTGAGAAGCC ACTATTTCTG 
GAACTTTGCA AACATATGGT CTTTGTGCAG CTACAAGAAG GGGAGCACGT ATTCCAACCT 
GGGGAGCCTG ACATCAGCAT CTATGTGGTT CAGGACGGAC GGCTGGAGGT CTGCATCCAA 
GACGCTGTGA CACAGGATGG CACCGAAGTG GTGGTCAAAG AGGTCCTGCC AGGGGACAGT 
GTCCACAGTC TCCTCAGCAT TTTGGATGTG ATCACCGGTC ACACTGCACC ATACAAAACA 
GTTTCTGCCC GTGCCGCTGT CTCGTCCACC GTGCTCTGGC TTCCTGCAGC AGCTTTTCAA 
GGAGTGTTTG AGAAATACCC TGAAACTCTG GTCAGGGTTG TGCAGATCAT CATGGTCCGG 
CTGCAGCGGG TGACTTTCCT GGCTCTGCAC AACTACCTGG GGCTGACTAC AGAGCTCTTC 
AACCCTGAGA GCCAGGCCAT CCCTCTCTTG TCAGTAGCAA GTGTGGCTGG ACGAGCCAAG 
AGACAGATGT CATATGGTCC AGAGGAGCAG CTTGAGAGGT CCCTACGGCC CTCGGAGTTC 
AGCAGCTCAG ATCATGGGAG CAGCTGTGTG ACAGTCTCTG GGCCTCTGCT GAAGAGGAGC 
TGCTCAGTGC CTCTGCCTTC TAACCATGGG GAGGTGGATG AGCTCAGACA GTCCCAGGGA 
AGTGGCTCTA ACACTTCTGC CTTCCAAGAA AGCCATGAGG GAGCCACCTC AGACCTGGGG 
ATGGCATACA ACCGTGCCAG GATTTTGCCA CACTCTGATG AGCAACTTGG GAACTCTTTG 
GCCAGCAAGT CCAAGAAAAG TGTTGTCGCA GAGACCCCCT CTGCCATCTT CCACTACTCA 
GAAAATTTCA GGGATGAGAC TGGGGCCTGT GGGAAGACAG ATGCTATCTT CAGAGCTGCC 
ACAAAGGACC TCCTTACCCT GATGAAGCTA GATGACCCAT CCCTGTTGGA TGGCCGGGTG 
GCATTTCTTC ATGTTCCTGC AGGCACCTTA GTGTCAAAAC AGGGAGATCA GGATGTCAAT 
ATTTTGTTTG TGGTCTCGGG GATGCTGCAT GTGTACCAGC AAAAGATTGA CAGCTTGGAG 
GATACTTGCC TGTTCCTCAC ACATCCCGGG GAGATGGTGG GTCAGCTGGC GGTGCTCACT 
GGAGAACCCC TAATGTTCAC CATCAGAGCC AACCGAGACT GCAGCTTTTT GTCCATCTCG 
AAGGCCCACT TCTATGAGAT CATGAGGAAA CGGCCGGATG TTGTCCTGGG CGTGGCACAC 
ACAGTTGTGA AGAGGATGTC ATCCTTTGTA CGGCAGATTG ATTTTGCTCT AGACTGGATG 
GAAGTAGAGG CTGGACGTGC CATATACAGG CAAGGAGACA AGTCTGACTG CACATACATC 
GTTCTCAGTG GCAGGCTGCG TTCTGTCATC CGAAAAGATG ATGGGAAAAA ACGCCTGGCA 
GGGGAATATG GCCGAGGAGA CCTTGTTGGT GTGGTGGAGA CGCTGACCCA CCAGGCCAGA 
GCAACCACAG TGCATGCTGT CCGGGACTCA GAACTGGCTA AGCTGCCAGC AGGAGCTCTG 
ACGTCTATCA AGCGCAGGTA CCCACAGGTT GTAACGCGAC TGATTCATCT CTTGGGCGAG 
AAGATCTTAG GCAGTCTCCA GCAGGGATCT GCAACAGGTC ACCAGCTTGG ATTTAACACA 
GCAAGCAGCA AATGGGACTT GGGGAACCCG CCTGGCAATC TGTCCACAGT GGCAGCACTG 
CCTGCTTCTG AGGATGTGCC CCTAACCGCC TTTGCTCTGG AGCTCCAACA TGCCCTCAGT 
GCCATCGGTC CTGTCCTGCT GCTGACCAGT GACAACATCA AACAACGCCT GGGTTCCGCT 
GCTCTAGACA GCATCCATGA GTACCGGCTC TCCAGTTGGC TGGGCCAGCA GGAGGACATC 
CACCGGATTG TGCTGTATCA GGCAGACGGC ACACTCACAC CGTGGACCCA GCGCTGCATC 
CGGCAGGCTG ACTGCATCCT CATCGTGGGC TTGGGTGAGC AAGAGCCAGC AGTAGGTGAG 
CTGGAGCAGA TGTTGGAGAG CACAGCTGTG CGTGCCCAGA AGCAGCTAAT CTTGCTGCAC 
AAAGAGGACG GTCCAGTGCC TTCCCGCACA GTGGAGTGGC TCAACATGCG AAGTTGGTGT 
TCTGGCCATT TGCACCTCTG CTGCCCACGC CGTGTCTTCT CCAAGAGAAG CTTGCCCAAG 
CTGGTCGAGA TGTACACACG TGTTTTCCAA CGACCACCAG ACCGTCATTC TGATTTTTCC 
CGCCTGGCAC GGATGCTGAC AGGCAATGCC ATTGCCTTGG TGCTTGGAGG GGGTGGAGCT 
AGAGGGTGTG CCCAGGTGGG CATCCTCAGG GCACTAGCAG AGTGTGGCGT CCCCGTGGAC 
ATAATCGGAG GCACATCCAT CGGGGCTTTC ATGGGTGCTC TGTTTGCTGA GGAGCGGAGC 
TACAGCCAAA CACGGATCCG GGCCAAGCAG TGGGCAGAGG GTATGACATC AATGATGAAG 
ACTATACTGG ACCTGACCTA CCCAATCACA TCCATGTTCT CTGGCACTGG CTTCAATAGC 
AGCATCAGCA ACATCTTCAA AGACCGGCAG ATTGAGGACC TGTGGCTCCC CTACTTTGCC 
ATCACCACCG ACATCACGGC CTCTGCCATG CGGGTTCACA CAGATGGCTC TCTGTGGAGG 
TATGTCCGTG CAAGCATGTC CTTGTCAGGC TATATGCCCC CACTCTGCGA CCCCAAGGAT 
GGACACCTGC TGATGGATGG TGGTTACATC AACAACCTCC CAGCGGATGT GGCAAGATCC 
ATGGGGGCAA AGGTTGTGAT TGCCATCGAT GTTGGCAGCC GGGACGAGAC AGACCTCACC 
AACTATGGCG ATGCCTTGTC TGGATGGTGG CTGCTTTGGA AACGCTGGAA CCCCTTGGCT 
ACAAAAGTCA AGGTGTTGAA CATGGCAGAG ATTCAGACTC GACTGGCCTA TGTGTGCTGC 
GTCCGGCAGC TGGAGATGGT GAAGAACAGC GACTACTGTG AGTACCTGAG GCCTCCCATC 
GACAGTTACC GCACCCTGGA CTTCGGCAAA TTTGATGAGA TCTGTGAAGT TGGGTATCAA 
CATGGGCGGA CAGTGTTTGA CATCTGGGTC CGGAGTGGTG TCCTAGAGAA GATGTTGCAG 
GACCAGCAGG GGACAAGCAA GAGGAAGGAC TGTGGGGTGT TCACCTGCCC CAATTCCTCC 
TTCACGGACC TTGCTGAGAT TGTGTCACGG ATTGAGCCGG CCAAGGTGGC TGCAGTGGAT 
GCCTTGCTTG GCACAGCCAC TGTCCTTGGA GAGAGGACCA ATGGAGCCCT CTCTTCTGCT 
CTAGATGAGT CTGACTATCA GACCGAGTAT GAGGAGGAAC TGCCAGCCAT CCCCAAGGAA 
ACGTATGCTG ACTTCCAGAG CACTGGGATT GAGCTAGACT CTGACTCGGT GAGGAATATG 
AGCCTTCGAT GTTGCAAGGA CCCCCCAGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla7 ( XM_011239092.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237394.1 Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X2, mRNA.

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CloneID	OMu74054
Clone ID Related Accession (Same CDS sequence)	XM_011239092.2 , XM_011239092.3
Accession Version	XM_011239092.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGTC TGCTGAGTTG TCTGGCTGGC TACTGTTTGG GTACCACCCT GAGCTCCTGG 
AGGCTGCATT TCATGGAGGA GCAGTCCCAG TCCACCATGC TGATGGGGAT TGGAATTGGC 
GCCCTCCTTA CCCTAGCCTT CGTTGGTATT ACCTTCTTTT TTGTATACAG AAGAGTGAGA 
AGATTACGAC GGGCAGAACC CACTCCTCAG TACAGGTTCC GGAAGAGAGA CAAAGTAATG 
TTTTATGGCC GGAAGATCAT GAGGAAGGTG ACCACACTCC CCCATACCTT GGTTGGGAAT 
ACCTCTGCAC CCCGGCAGCG AGTCAGGAAG AGGACCAAAG TGCTGTCTTT AGCCAAGAGG 
ATTCTGCGCT TTAAGAAAGA GTATCCCACT TTGCAGCCAA AAGAGCCTCC GCCCTCCCTG 
CTTGAGGCTG ATCTTACAGA GTTTGATGTG AAGAACTCTC ATCTGCCATC AGAAGTCTTG 
TACATGTTGA AAAATGTTCG GGTTCTAGGC CACTTTGAGA AGCCACTATT TCTGGAACTT 
TGCAAACATA TGGTCTTTGT GCAGCTACAA GAAGGGGAGC ACGTATTCCA ACCTGGGGAG 
CCTGACATCA GCATCTATGT GGTTCAGGAC GGACGGCTGG AGGTCTGCAT CCAAGACGCT 
GTGACACAGG ATGGCACCGA AGTGGTGGTC AAAGAGGTCC TGCCAGGGGA CAGTGTCCAC 
AGTCTCCTCA GCATTTTGGA TGTGATCACC GGTCACACTG CACCATACAA AACAGTTTCT 
GCCCGTGCCG CTGTCTCGTC CACCGTGCTC TGGCTTCCTG CAGCAGCTTT TCAAGGAGTG 
TTTGAGAAAT ACCCTGAAAC TCTGGTCAGG GTTGTGCAGA TCATCATGGT CCGGCTGCAG 
CGGGTGACTT TCCTGGCTCT GCACAACTAC CTGGGGCTGA CTACAGAGCT CTTCAACCCT 
GAGAGCCAGG CCATCCCTCT CTTGTCAGTA GCAAGTGTGG CTGGACGAGC CAAGAGACAG 
ATGTCATATG GTCCAGAGGA GCAGCTTGAG AGGTCCCTAC GGCCCTCGGA GTTCAGCAGC 
TCAGATCATG GGAGCAGCTG TGTGACAGTC TCTGGGCCTC TGCTGAAGAG GAGCTGCTCA 
GTGCCTCTGC CTTCTAACCA TGGGGAGGTG GATGAGCTCA GACAGTCCCA GGGAAGTGGC 
TCTAACACTT CTGCCTTCCA AGAAAGCCAT GAGGGAGCCA CCTCAGACCT GGGGATGGCA 
TACAACCGTG CCAGGATTTT GCCACACTCT GATGAGCAAC TTGGGAACTC TTTGGCCAGC 
AAGTCCAAGA AAAGTGTTGT CGCAGAGACC CCCTCTGCCA TCTTCCACTA CTCAGAAAAT 
TTCAGGGATG AGACTGGGGC CTGTGGGAAG ACAGATGCTA TCTTCAGAGC TGCCACAAAG 
GACCTCCTTA CCCTGATGAA GCTAGATGAC CCATCCCTGT TGGATGGCCG GGTGGCATTT 
CTTCATGTTC CTGCAGGCAC CTTAGTGTCA AAACAGGGAG ATCAGGATGT CAATATTTTG 
TTTGTGGTCT CGGGGATGCT GCATGTGTAC CAGCAAAAGA TTGACAGCTT GGAGGATACT 
TGCCTGTTCC TCACACATCC CGGGGAGATG GTGGGTCAGC TGGCGGTGCT CACTGGAGAA 
CCCCTAATGT TCACCATCAG AGCCAACCGA GACTGCAGCT TTTTGTCCAT CTCGAAGGCC 
CACTTCTATG AGATCATGAG GAAACGGCCG GATGTTGTCC TGGGCGTGGC ACACACAGTT 
GTGAAGAGGA TGTCATCCTT TGTACGGCAG ATTGATTTTG CTCTAGACTG GATGGAAGTA 
GAGGCTGGAC GTGCCATATA CAGGCAAGGA GACAAGTCTG ACTGCACATA CATCGTTCTC 
AGTGGCAGGC TGCGTTCTGT CATCCGAAAA GATGATGGGA AAAAACGCCT GGCAGGGGAA 
TATGGCCGAG GAGACCTTGT TGGTGTGGTG GAGACGCTGA CCCACCAGGC CAGAGCAACC 
ACAGTGCATG CTGTCCGGGA CTCAGAACTG GCTAAGCTGC CAGCAGGAGC TCTGACGTCT 
ATCAAGCGCA GGTACCCACA GGTTGTAACG CGACTGATTC ATCTCTTGGG CGAGAAGATC 
TTAGGCAGTC TCCAGCAGGG ATCTGCAACA GGTCACCAGC TTGGATTTAA CACAGCAAGC 
AGCAAATGGG ACTTGGGGAA CCCGCCTGGC AATCTGTCCA CAGTGGCAGC ACTGCCTGCT 
TCTGAGGATG TGCCCCTAAC CGCCTTTGCT CTGGAGCTCC AACATGCCCT CAGTGCCATC 
GGTCCTGTCC TGCTGCTGAC CAGTGACAAC ATCAAACAAC GCCTGGGTTC CGCTGCTCTA 
GACAGCATCC ATGAGTACCG GCTCTCCAGT TGGCTGGGCC AGCAGGAGGA CATCCACCGG 
ATTGTGCTGT ATCAGGCAGA CGGCACACTC ACACCGTGGA CCCAGCGCTG CATCCGGCAG 
GCTGACTGCA TCCTCATCGT GGGCTTGGGT GAGCAAGAGC CAGCAGTAGG TGAGCTGGAG 
CAGATGTTGG AGAGCACAGC TGTGCGTGCC CAGAAGCAGC TAATCTTGCT GCACAAAGAG 
GACGGTCCAG TGCCTTCCCG CACAGTGGAG TGGCTCAACA TGCGAAGTTG GTGTTCTGGC 
CATTTGCACC TCTGCTGCCC ACGCCGTGTC TTCTCCAAGA GAAGCTTGCC CAAGCTGGTC 
GAGATGTACA CACGTGTTTT CCAACGACCA CCAGACCGTC ATTCTGATTT TTCCCGCCTG 
GCACGGATGC TGACAGGCAA TGCCATTGCC TTGGTGCTTG GAGGGGGTGG AGCTAGAGGG 
TGTGCCCAGG TGGGCATCCT CAGGGCACTA GCAGAGTGTG GCGTCCCCGT GGACATAATC 
GGAGGCACAT CCATCGGGGC TTTCATGGGT GCTCTGTTTG CTGAGGAGCG GAGCTACAGC 
CAAACACGGA TCCGGGCCAA GCAGTGGGCA GAGGGTATGA CATCAATGAT GAAGACTATA 
CTGGACCTGA CCTACCCAAT CACATCCATG TTCTCTGGCA CTGGCTTCAA TAGCAGCATC 
AGCAACATCT TCAAAGACCG GCAGATTGAG GACCTGTGGC TCCCCTACTT TGCCATCACC 
ACCGACATCA CGGCCTCTGC CATGCGGGTT CACACAGATG GCTCTCTGTG GAGGTATGTC 
CGTGCAAGCA TGTCCTTGTC AGGCTATATG CCCCCACTCT GCGACCCCAA GGATGGACAC 
CTGCTGATGG ATGGTGGTTA CATCAACAAC CTCCCAGCGG ATGTGGCAAG ATCCATGGGG 
GCAAAGGTTG TGATTGCCAT CGATGTTGGC AGCCGGGACG AGACAGACCT CACCAACTAT 
GGCGATGCCT TGTCTGGATG GTGGCTGCTT TGGAAACGCT GGAACCCCTT GGCTACAAAA 
GTCAAGGTGT TGAACATGGC AGAGATTCAG ACTCGACTGG CCTATGTGTG CTGCGTCCGG 
CAGCTGGAGA TGGTGAAGAA CAGCGACTAC TGTGAGTACC TGAGGCCTCC CATCGACAGT 
TACCGCACCC TGGACTTCGG CAAATTTGAT GAGATCTGTG AAGTTGGGTA TCAACATGGG 
CGGACAGTGT TTGACATCTG GGTCCGGAGT GGTGTCCTAG AGAAGATGTT GCAGGACCAG 
CAGGGGACAA GCAAGAGGAA GGACTGTGGG GTGTTCACCT GCCCCAATTC CTCCTTCACG 
GACCTTGCTG AGATTGTGTC ACGGATTGAG CCGGCCAAGG TGGCTGCAGT GGATGCCTTG 
CTTGGCACAG CCACTGTCCT TGGAGAGAGG ACCAATGGAG CCCTCTCTTC TGCTCTAGAT 
GAGTCTGACT ATCAGACCGA GTATGAGGAG GAACTGCCAG CCATCCCCAA GGAAACGTAT 
GCTGACTTCC AGAGCACTGG GATTGAGCTA GACTCTGACT CGGAATATGA GCCTTCGATG 
TTGCAAGGAC CCCCCAGCTT GACTTCCCCA GAACAGTCCC AGGACTCCTT CCCTTGGCTC 
CCTAATCAAG ATGACCAGGG GCCAAGACTG GAACATCCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237394.1
CDS	104..4228
Translation	MPGLLSCLAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGALLTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGNTSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSEVLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVCIQDAVTQDGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAFQGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAGRAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDELRQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETPSAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLVSKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIRANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRAIYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVHAVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASSKWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAALDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVGELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRSLPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAECGVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMFSGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGYMPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGWWLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLDFGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLAEIVSRIEPAKVAAVDALLGTATVLGERTNGALSSALDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQGPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS

Target ORF information:

RefSeq Version	XM_011239092.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 7 (Pnpla7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239092.2

ORF Insert Sequence:

ATGCCTGGTC TGCTGAGTTG TCTGGCTGGC TACTGTTTGG GTACCACCCT GAGCTCCTGG 
AGGCTGCATT TCATGGAGGA GCAGTCCCAG TCCACCATGC TGATGGGGAT TGGAATTGGC 
GCCCTCCTTA CCCTAGCCTT CGTTGGTATT ACCTTCTTTT TTGTATACAG AAGAGTGAGA 
AGATTACGAC GGGCAGAACC CACTCCTCAG TACAGGTTCC GGAAGAGAGA CAAAGTAATG 
TTTTATGGCC GGAAGATCAT GAGGAAGGTG ACCACACTCC CCCATACCTT GGTTGGGAAT 
ACCTCTGCAC CCCGGCAGCG AGTCAGGAAG AGGACCAAAG TGCTGTCTTT AGCCAAGAGG 
ATTCTGCGCT TTAAGAAAGA GTATCCCACT TTGCAGCCAA AAGAGCCTCC GCCCTCCCTG 
CTTGAGGCTG ATCTTACAGA GTTTGATGTG AAGAACTCTC ATCTGCCATC AGAAGTCTTG 
TACATGTTGA AAAATGTTCG GGTTCTAGGC CACTTTGAGA AGCCACTATT TCTGGAACTT 
TGCAAACATA TGGTCTTTGT GCAGCTACAA GAAGGGGAGC ACGTATTCCA ACCTGGGGAG 
CCTGACATCA GCATCTATGT GGTTCAGGAC GGACGGCTGG AGGTCTGCAT CCAAGACGCT 
GTGACACAGG ATGGCACCGA AGTGGTGGTC AAAGAGGTCC TGCCAGGGGA CAGTGTCCAC 
AGTCTCCTCA GCATTTTGGA TGTGATCACC GGTCACACTG CACCATACAA AACAGTTTCT 
GCCCGTGCCG CTGTCTCGTC CACCGTGCTC TGGCTTCCTG CAGCAGCTTT TCAAGGAGTG 
TTTGAGAAAT ACCCTGAAAC TCTGGTCAGG GTTGTGCAGA TCATCATGGT CCGGCTGCAG 
CGGGTGACTT TCCTGGCTCT GCACAACTAC CTGGGGCTGA CTACAGAGCT CTTCAACCCT 
GAGAGCCAGG CCATCCCTCT CTTGTCAGTA GCAAGTGTGG CTGGACGAGC CAAGAGACAG 
ATGTCATATG GTCCAGAGGA GCAGCTTGAG AGGTCCCTAC GGCCCTCGGA GTTCAGCAGC 
TCAGATCATG GGAGCAGCTG TGTGACAGTC TCTGGGCCTC TGCTGAAGAG GAGCTGCTCA 
GTGCCTCTGC CTTCTAACCA TGGGGAGGTG GATGAGCTCA GACAGTCCCA GGGAAGTGGC 
TCTAACACTT CTGCCTTCCA AGAAAGCCAT GAGGGAGCCA CCTCAGACCT GGGGATGGCA 
TACAACCGTG CCAGGATTTT GCCACACTCT GATGAGCAAC TTGGGAACTC TTTGGCCAGC 
AAGTCCAAGA AAAGTGTTGT CGCAGAGACC CCCTCTGCCA TCTTCCACTA CTCAGAAAAT 
TTCAGGGATG AGACTGGGGC CTGTGGGAAG ACAGATGCTA TCTTCAGAGC TGCCACAAAG 
GACCTCCTTA CCCTGATGAA GCTAGATGAC CCATCCCTGT TGGATGGCCG GGTGGCATTT 
CTTCATGTTC CTGCAGGCAC CTTAGTGTCA AAACAGGGAG ATCAGGATGT CAATATTTTG 
TTTGTGGTCT CGGGGATGCT GCATGTGTAC CAGCAAAAGA TTGACAGCTT GGAGGATACT 
TGCCTGTTCC TCACACATCC CGGGGAGATG GTGGGTCAGC TGGCGGTGCT CACTGGAGAA 
CCCCTAATGT TCACCATCAG AGCCAACCGA GACTGCAGCT TTTTGTCCAT CTCGAAGGCC 
CACTTCTATG AGATCATGAG GAAACGGCCG GATGTTGTCC TGGGCGTGGC ACACACAGTT 
GTGAAGAGGA TGTCATCCTT TGTACGGCAG ATTGATTTTG CTCTAGACTG GATGGAAGTA 
GAGGCTGGAC GTGCCATATA CAGGCAAGGA GACAAGTCTG ACTGCACATA CATCGTTCTC 
AGTGGCAGGC TGCGTTCTGT CATCCGAAAA GATGATGGGA AAAAACGCCT GGCAGGGGAA 
TATGGCCGAG GAGACCTTGT TGGTGTGGTG GAGACGCTGA CCCACCAGGC CAGAGCAACC 
ACAGTGCATG CTGTCCGGGA CTCAGAACTG GCTAAGCTGC CAGCAGGAGC TCTGACGTCT 
ATCAAGCGCA GGTACCCACA GGTTGTAACG CGACTGATTC ATCTCTTGGG CGAGAAGATC 
TTAGGCAGTC TCCAGCAGGG ATCTGCAACA GGTCACCAGC TTGGATTTAA CACAGCAAGC 
AGCAAATGGG ACTTGGGGAA CCCGCCTGGC AATCTGTCCA CAGTGGCAGC ACTGCCTGCT 
TCTGAGGATG TGCCCCTAAC CGCCTTTGCT CTGGAGCTCC AACATGCCCT CAGTGCCATC 
GGTCCTGTCC TGCTGCTGAC CAGTGACAAC ATCAAACAAC GCCTGGGTTC CGCTGCTCTA 
GACAGCATCC ATGAGTACCG GCTCTCCAGT TGGCTGGGCC AGCAGGAGGA CATCCACCGG 
ATTGTGCTGT ATCAGGCAGA CGGCACACTC ACACCGTGGA CCCAGCGCTG CATCCGGCAG 
GCTGACTGCA TCCTCATCGT GGGCTTGGGT GAGCAAGAGC CAGCAGTAGG TGAGCTGGAG 
CAGATGTTGG AGAGCACAGC TGTGCGTGCC CAGAAGCAGC TAATCTTGCT GCACAAAGAG 
GACGGTCCAG TGCCTTCCCG CACAGTGGAG TGGCTCAACA TGCGAAGTTG GTGTTCTGGC 
CATTTGCACC TCTGCTGCCC ACGCCGTGTC TTCTCCAAGA GAAGCTTGCC CAAGCTGGTC 
GAGATGTACA CACGTGTTTT CCAACGACCA CCAGACCGTC ATTCTGATTT TTCCCGCCTG 
GCACGGATGC TGACAGGCAA TGCCATTGCC TTGGTGCTTG GAGGGGGTGG AGCTAGAGGG 
TGTGCCCAGG TGGGCATCCT CAGGGCACTA GCAGAGTGTG GCGTCCCCGT GGACATAATC 
GGAGGCACAT CCATCGGGGC TTTCATGGGT GCTCTGTTTG CTGAGGAGCG GAGCTACAGC 
CAAACACGGA TCCGGGCCAA GCAGTGGGCA GAGGGTATGA CATCAATGAT GAAGACTATA 
CTGGACCTGA CCTACCCAAT CACATCCATG TTCTCTGGCA CTGGCTTCAA TAGCAGCATC 
AGCAACATCT TCAAAGACCG GCAGATTGAG GACCTGTGGC TCCCCTACTT TGCCATCACC 
ACCGACATCA CGGCCTCTGC CATGCGGGTT CACACAGATG GCTCTCTGTG GAGGTATGTC 
CGTGCAAGCA TGTCCTTGTC AGGCTATATG CCCCCACTCT GCGACCCCAA GGATGGACAC 
CTGCTGATGG ATGGTGGTTA CATCAACAAC CTCCCAGCGG ATGTGGCAAG ATCCATGGGG 
GCAAAGGTTG TGATTGCCAT CGATGTTGGC AGCCGGGACG AGACAGACCT CACCAACTAT 
GGCGATGCCT TGTCTGGATG GTGGCTGCTT TGGAAACGCT GGAACCCCTT GGCTACAAAA 
GTCAAGGTGT TGAACATGGC AGAGATTCAG ACTCGACTGG CCTATGTGTG CTGCGTCCGG 
CAGCTGGAGA TGGTGAAGAA CAGCGACTAC TGTGAGTACC TGAGGCCTCC CATCGACAGT 
TACCGCACCC TGGACTTCGG CAAATTTGAT GAGATCTGTG AAGTTGGGTA TCAACATGGG 
CGGACAGTGT TTGACATCTG GGTCCGGAGT GGTGTCCTAG AGAAGATGTT GCAGGACCAG 
CAGGGGACAA GCAAGAGGAA GGACTGTGGG GTGTTCACCT GCCCCAATTC CTCCTTCACG 
GACCTTGCTG AGATTGTGTC ACGGATTGAG CCGGCCAAGG TGGCTGCAGT GGATGCCTTG 
CTTGGCACAG CCACTGTCCT TGGAGAGAGG ACCAATGGAG CCCTCTCTTC TGCTCTAGAT 
GAGTCTGACT ATCAGACCGA GTATGAGGAG GAACTGCCAG CCATCCCCAA GGAAACGTAT 
GCTGACTTCC AGAGCACTGG GATTGAGCTA GACTCTGACT CGGAATATGA GCCTTCGATG 
TTGCAAGGAC CCCCCAGCTT GACTTCCCCA GAACAGTCCC AGGACTCCTT CCCTTGGCTC 
CCTAATCAAG ATGACCAGGG GCCAAGACTG GAACATCCTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Degradation of mouse NTE-related esterase by macroautophagy and the proteasome.
Molecular biology reports39(6)7125-31(2012 Jun)
Chang PA,Chen YY,Long DX,Qin WZ,Mou XL

Identification of two novel splicing variants of murine NTE-related esterase.
Gene497(2)164-71(2012 Apr)
Chang PA,Wang ZX,Long DX,Qin WZ,Wei CY,Wu YJ

Identification and characterization of a splice variant of the catalytic domain of mouse NTE-related esterase.
Gene417(1-2)43-50(2008 Jul)
Chang PA,Long DX,Sun Q,Wang Q,Bu YQ,Wu YJ

Identification of an insulin-regulated lysophospholipase with homology to neuropathy target esterase.
The Journal of biological chemistry283(9)5908-17(2008 Feb)
Kienesberger PC,Lass A,Preiss-Landl K,Wolinski H,Kohlwein SD,Zimmermann R,Zechner R

Molecular cloning and expression of the C-terminal domain of mouse NTE-related esterase.
Molecular and cellular biochemistry306(1-2)25-32(2007 Dec)
Chang PA,Long DX,Wu YJ

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Pnpla7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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