sws cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sws
Entrez Gene ID	31716
Full Name	swiss cheese
Synonyms	CG2212,Dmel\CG2212,PNPLA6,SWS,Sws,olfE
General protein information	Preferred Names swiss cheese Names CG2212 gene product from transcript CG2212-RC CG2212-PA CG2212-PB CG2212-PC olfactory E olfactory-E swiss-cheese swisscheese sws-PA sws-PB sws-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7D1-7D1\|1-22 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078520.3	NP_511075.3	swiss cheese, isoform A
NM_167141.3	NP_727225.1	swiss cheese, isoform B
NM_167140.2	NP_727224.2	swiss cheese, isoform C

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PNPLA7	XP_003586736.2
Mus musculus (house mouse)	Pnpla7	NP_666363.3
Rattus norvegicus (Norway rat)	Pnpla7	NP_653339.2
Xenopus tropicalis (tropical clawed frog)	pnpla6	NP_001090744.2
Homo sapiens (human)	PNPLA7	NP_001092007.1
Canis lupus familiaris (dog)	PNPLA7	XP_537782.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000467	XP_310627.5
Caenorhabditis elegans (roundworm)	ZK370.4	NP_498926.2
Drosophila melanogaster (fruit fly)	sws	NP_511075.3
	LOC721508	XP_001117679.2
Gallus gallus (chicken)	PNPLA7	NP_001124214.1
Danio rerio (zebrafish)	pnpla7b	XP_692276.5
Caenorhabditis elegans (roundworm)	M110.7	NP_495734.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the loss of SWS in glia impairs neuronal function.
Title: Glial expression of Swiss cheese (SWS), the Drosophila orthologue of neuropathy target esterase (NTE), is required for neuronal ensheathment and function.

inducible, neuron-specific expression of full-length human wildtype NTE reduces vacuole formation and substantially rescues mobility.
Title: Delayed Induction of Human NTE (PNPLA6) Rescues Neurodegeneration and Mobility Defects of Drosophila swiss cheese (sws) Mutants.

OP-modified SWS cannot release PKA-C3 and the resulting loss of PKA-C3 activity plays a crucial role in developing the delayed symptoms of organophosphate-induced delayed neuropathy but not in the acute toxicity
Title: Organophosphate-induced changes in the PKA regulatory function of Swiss Cheese/NTE lead to behavioral deficits and neurodegeneration.

The following sws gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sws cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01063	NM_167141.3 Latest version!	Drosophila melanogaster swiss cheese (sws), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01061	NM_167140.2 Latest version!	Drosophila melanogaster swiss cheese (sws), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa01062	NM_078520.3 Latest version!	Drosophila melanogaster swiss cheese (sws), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sws ( NM_167141.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727225.1 Drosophila melanogaster swiss cheese (sws), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01063
Clone ID Related Accession (Same CDS sequence)	NM_167141.3
Accession Version	NM_167141.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	swiss cheese, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167141.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGATTG CCGGCGTCTT CCCACCATTT TGTGATTACC GCGACGGCCA CCTCCTGCTC 
GATGGCTGCT ACACGAACAA CGTGCCAGCC GATGTGATGC ATAATCTTGG AGCTGCTCAC 
ATAATCGCCA TCGATGTGGG ATCCCAGGAT GACACTGACC TGACCAACTA TGGCGACGAC 
CTAAGCGGTT GGTGGCTGCT CTACAAGAAA TGGAATCCCT TCACATCGCC TGTGAAGGTG 
CCCGACTTGC CGGACATCCA ATCCCGACTA GCCTATGTGT CCTGCGTACG CCAACTAGAG 
GAGGTCAAAA ACTCCGACTA CTGCGAGTAT ATCCGACCGC CGATCGATAA GTACAAGACT 
CTAGCCTTCG GCAGCTTCGA CGAAATTCGA GACGTGGGCT ATGTGTTTGG CAAAAACTAC 
TTTGAGAGCA TGGCCAAGGC TGGTCGATTG GGCCGCTTCA ATCAGTGGTT CAACAAAGAG 
CCACCCAAGC GGGTTAACCA TGCCTCCCTC AACGAGTACA CGTTCATCGA TCTGGCGCAG 
ATCGTGTGCC GTCTGCCGGA GACATACGCC GTGAATACCG CGGAACTCTT TAGCGAGGAC 
GAAGACTGTG ATGGCTACAT TTCGGAGCCG ACCACACTGA ATACGGATCG GCGTCGCATC 
CAGGTGTCGC GCGCTGGCAA CTCGTTATCC TTTTCCGAAA CGGAAATGGA CTCGGACGTG 
GAGCTTGACC TGAAGCTGGA GCGCAAGACG GACAAGTCCA CACAGTCGTC GCCGCCGAGT 
AACAGCAGGT CGGACATGCG CGGCAAGGAA GAGGCTAGAC ATATGTCTAA TTGGCATTGG 
GGTGTGAAGC ACAAGGATGA GACTGGCAGC GGAGCCACTG AAGCCACAAA AACACAAACC 
GGTCAGGAGC AGGAACTCCA GCAGGAGCAA CAGGATCAGG GAGCTACGGC TGAGCAGCTG 
GTGGATAAAG ACAAGGAGGA GAACAAAGAG AACAGAAGTA GCCCGAATAA CGAAACCAAA 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727225.1
CDS	305..1330
Translation	MSIAGVFPPFCDYRDGHLLLDGCYTNNVPADVMHNLGAAHIIAIDVGSQDDTDLTNYGDDLSGWWLLYKKWNPFTSPVKVPDLPDIQSRLAYVSCVRQLEEVKNSDYCEYIRPPIDKYKTLAFGSFDEIRDVGYVFGKNYFESMAKAGRLGRFNQWFNKEPPKRVNHASLNEYTFIDLAQIVCRLPETYAVNTAELFSEDEDCDGYISEPTTLNTDRRRIQVSRAGNSLSFSETEMDSDVELDLKLERKTDKSTQSSPPSNSRSDMRGKEEARHMSNWHWGVKHKDETGSGATEATKTQTGQEQELQQEQQDQGATAEQLVDKDKEENKENRSSPNNETKN

Target ORF information:

RefSeq Version	NM_167141.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster swiss cheese (sws), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167141.3

ORF Insert Sequence:

ATGTCGATTG CCGGCGTCTT CCCACCATTT TGTGATTACC GCGACGGCCA CCTCCTGCTC 
GATGGCTGCT ACACGAACAA CGTGCCAGCC GATGTGATGC ATAATCTTGG AGCTGCTCAC 
ATAATCGCCA TCGATGTGGG ATCCCAGGAT GACACTGACC TGACCAACTA TGGCGACGAC 
CTAAGCGGTT GGTGGCTGCT CTACAAGAAA TGGAATCCCT TCACATCGCC TGTGAAGGTG 
CCCGACTTGC CGGACATCCA ATCCCGACTA GCCTATGTGT CCTGCGTACG CCAACTAGAG 
GAGGTCAAAA ACTCCGACTA CTGCGAGTAT ATCCGACCGC CGATCGATAA GTACAAGACT 
CTAGCCTTCG GCAGCTTCGA CGAAATTCGA GACGTGGGCT ATGTGTTTGG CAAAAACTAC 
TTTGAGAGCA TGGCCAAGGC TGGTCGATTG GGCCGCTTCA ATCAGTGGTT CAACAAAGAG 
CCACCCAAGC GGGTTAACCA TGCCTCCCTC AACGAGTACA CGTTCATCGA TCTGGCGCAG 
ATCGTGTGCC GTCTGCCGGA GACATACGCC GTGAATACCG CGGAACTCTT TAGCGAGGAC 
GAAGACTGTG ATGGCTACAT TTCGGAGCCG ACCACACTGA ATACGGATCG GCGTCGCATC 
CAGGTGTCGC GCGCTGGCAA CTCGTTATCC TTTTCCGAAA CGGAAATGGA CTCGGACGTG 
GAGCTTGACC TGAAGCTGGA GCGCAAGACG GACAAGTCCA CACAGTCGTC GCCGCCGAGT 
AACAGCAGGT CGGACATGCG CGGCAAGGAA GAGGCTAGAC ATATGTCTAA TTGGCATTGG 
GGTGTGAAGC ACAAGGATGA GACTGGCAGC GGAGCCACTG AAGCCACAAA AACACAAACC 
GGTCAGGAGC AGGAACTCCA GCAGGAGCAA CAGGATCAGG GAGCTACGGC TGAGCAGCTG 
GTGGATAAAG ACAAGGAGGA GAACAAAGAG AACAGAAGTA GCCCGAATAA CGAAACCAAA 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sws ( NM_167140.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727224.2 Drosophila melanogaster swiss cheese (sws), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01061
Clone ID Related Accession (Same CDS sequence)	NM_167140.2
Accession Version	NM_167140.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	swiss cheese, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 18, 2009 this sequence version replaced NM_167140.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGTCC TGGAAATGCT GCGCGCCAGC GCCAGCGGCA GCTACAACAC AATATTCTCG 
GACGCGTGGT GCCAATATGT CTCCAAGCAG ATCACAGCAA CGGTGTACAT GTATTTCGCC 
CTGGTAATGA TGAGCCTGCT CTTCATAGCC TGGTTCTTAT ACTTCAAACG AATGGCACGT 
TTGCGTTTGC GCGACGAGAT TGCCCGTTCC ATTAGCACCG TAACCAATTC ATCCGGCGAT 
ATGCGTGGCC TGCGTTTCCG GAAGCGTGAC AAGATGCTAT TCTACGGCCG TCGAATGCTG 
CGCAAAATGA AGAACGTGAG CGGTCAAATG TACAGCAGCG GTAAGGGCTA CAAGCGTCGT 
GCTGTCATGC GTTTCGCGAG AAGGATACTC CAGCTGAGGC GGGATAATAT GCCGCTAGAG 
ATGCGCACCG TGGAGCCGCC AGCCGAGTAT CTGGAGGAGA CAATCGAGGG CAGCGATAGA 
GTTCCGCCCG ATGCACTCTA TATGCTCCAG AGCATTCGCA TTTTTGGACA TTTCGAGAAG 
CCGGTCTTTT TGCGGCTCTG CAAGCACACC CAATTGCTGG AACTGATGGC CGGCGACTAT 
TTGTTTAAGA TAACCGATCC GGACGATAGC GTCTATATCG TTCAATCGGG CATGATCAAT 
GTGTACATCT CCAATGCCGA TGGCAGCACA CTATCATTGA AGACGGTCAG GAAGGGCGAG 
TCGGTGACAT CGCTGCTCAG CTTTATTGAT GTCCTGTCGG GGAATCCCAG CTACTATAAG 
ACGGTCACCG CCAAAGCCAT TGAGAAATCG GTGGTCATTC GTCTGCCCAT GCAGGCATTC 
GAAGAGGTGT TCCAGGACAA TCCGGACGTA ATGATTCGCG TCATCCAGGT GATTATGATC 
CGGCTACAAC GCGTACTGTT CACCGCTTTG CGCAACTACC TCGGTCTAAA TGCCGAACTG 
GTGCAGAATC ACATGCGCTA CAAGAGCGTA TCCACAATGA GTGGACCGAT TAACTCGCAG 
ACCTCGCAAT CCTCACGCCA GGCGCCCAAT GGGCCGCCCA TGGTGATCAG CCAGATGAAT 
CTGATGCAGT CCGCTGTTTC GGGAACGGGA TCATCGGGTG TCTCGGTGAC CGTAACTCGA 
CCACCATCCT CGCCATCGCG ACACTCGCGC GAGGAGCACA CGCTATCCGA TCCGAATCCA 
AATCCGGATG GTAGTTTTCA CGGCACTACC AATCTGTTCA CCGAGGTGCA TGGCGATGCG 
CCCAATGCAG ATCTATTCCA TCAGCAGCAG CAGCAGCATT CGGTGGGCAA CCTATCCACG 
CGTCGCAGTT CCATAACTCT GATGGCACCC GATGGTTCGC ACTCGTGTCT ACAGACACCC 
GGTGTGACAA CCTCGATAGA CATGCGCCTG GTGCAATCCT CAGCGGTGGA CAGCCTACGC 
AAGGAATTGG GTCTGTCCGA GGAGGATTCC CACATCATTG AGCCTTTTGT AGAGTTAAGG 
GAACTGGAGC CGAATGTCAC CCTGATTACG GAGGGCAATG CGGACGATGT TTGCGTTTGG 
TTTGTGATGA CGGGCACATT GGCTGTTTAC CAGAGCAATC AGGATGCCAC GCGGGCCAAG 
CAGGATAAGA GCGACATGCT CATTCACTTT GTGCATCCCG GCGAAATCGT TGGTGGACTG 
GCCATGCTAA CGGGAGAGGC CAGTGCGTAT ACCATTCGAT CGAGAAGCAT CACCAGAATA 
GCCTTCATCC GACGAGCAGC TATTTACCAG ATCATGCGAC AACGACCACG CATTGTTTTG 
GATCTGGGCA ACGGAGTAGT ACGCCGTCTA TCGCCTCTGG TGAGGCAGTG TGACTATGCC 
TTGGACTGGA TCTTTCTGGA ATCGGGCAGA GCTGTCTATC GGCAGGACGA GAGCAGCGAC 
AGCACATATA TTGTGCTTAG CGGGCGAATG CGTTCGGTTA TCACGCATCC TGGTGGCAAA 
AAGGAGATCG TCGGCGAATA CGGCAAGGGG GATCTTGTGG GCATCGTTGA AATGATCACG 
GAAACGTCGC GCACCACGAC GGTGATGGCT GTGCGCGACT CGGAGCTGGC CAAGCTGCCC 
GAGGGCCTGT TCAATGCCAT CAAGCTGCGC TACCCCATCG TGGTGACCAA GCTGATTAGC 
TTCCTTAGCC ACCGCTTCCT CGGCTCGATG CAGACGCGCT CGGGCAGCGG GGCGCCGGGC 
GCGCCCGTCG AGGCCAATCC CGTCACGCAC AAGTACTCCA CGGTGGCCCT GGTGCCCATC 
ACCGACGAGG TGCCGATGAC GCCCTTCACC TACGAGCTCT ACCACTCGCT CTGTGCCATA 
GGACCCGTTC TCCGTTTGAC CTCCGATGTG GTGCGCAAGC AGCTGGGCTC CAACATCTTT 
GAGGCAGCCA ACGAGTATCG TCTAACCTCG TGGCTGGCGC AGCAGGAGGA CCGAAACATC 
ATCACCCTCT ATCAGTGTGA CAGTTCGCTG AGCGCGTGGA CACAGCGCTG CATGCGACAG 
GCTGACGTTA TCCTGATCGT GGGCCTCGGC GATCGATCAC ACCTGGTCGG AAAGTTCGAA 
CGCGAGATCG ACCGGCTGGC GATGCGAACG CAAAAGGAGC TCGTCCTGCT ATATCCGGAG 
GCCAGCAATG CCAAGCCGGC CAACACGCTT AGTTGGCTAA ATGCCCGACC CTGGGTAACA 
AAGCACCACC ATGTGCTCTG CGTCAAGCGG ATCTTCACGC GCAAAAGTCA ATATCGCATT 
AATGATCTCT ATAGTCGCGT CCTGCTGTCC GAGCCAAACA TGCATTCCGA CTTCTCGCGT 
TTGGCGCGCT GGCTTACGGG CAACTCGATT GGCCTGGTGC TGGGCGGCGG AGGAGCGCGT 
GGAGCTGCCC ACATTGGTAT GCTGAAGGCC ATCCAGGAGG CGGGCATACC TGTTGACATG 
GTGGGCGGCG TCAGCATCGG TGCCTTGATG GGAGCTCTCT GGTGCTCGGA GCGTAATATC 
ACCACGGTAA CGCAGAAGGC GCGCGAGTGG TCAAAGAAAA TGACGAAATG GTTCCTACAA 
CTACTTGACC TCACCTACCC GATCACATCG ATGTTCTCCG GACGAGAGTT CAACAAGACG 
ATCCACGACA CTTTCGGGGA CGTGAGCATC GAGGACCTGT GGATACCCTA CTTCACCCTC 
ACCACCGACA TAACCGCCAG TTGCCATCGC ATTCACACCA ATGGCCACCT GTGGCGGTAC 
TGCCGCGCCA GCATGTCGAT TGCCGGCGTC TTCCCACCAT TTTGTGATTA CCGCGACGGC 
CACCTCCTGC TCGATGGCTG CTACACGAAC AACGTGCCAG CCGATGTGAT GCATAATCTT 
GGAGCTGCTC ACATAATCGC CATCGATGTG GGATCCCAGG ATGACACTGA CCTGACCAAC 
TATGGCGACG ACCTAAGCGG TTGGTGGCTG CTCTACAAGA AATGGAATCC CTTCACATCG 
CCTGTGAAGG TGCCCGACTT GCCGGACATC CAATCCCGAC TAGCCTATGT GTCCTGCGTA 
CGCCAACTAG AGGAGGTCAA AAACTCCGAC TACTGCGAGT ATATCCGACC GCCGATCGAT 
AAGTACAAGA CTCTAGCCTT CGGCAGCTTC GACGAAATTC GAGACGTGGG CTATGTGTTT 
GGCAAAAACT ACTTTGAGAG CATGGCCAAG GCTGGTCGAT TGGGCCGCTT CAATCAGTGG 
TTCAACAAAG AGCCACCCAA GCGGGTTAAC CATGCCTCCC TCAACGAGTA CACGTTCATC 
GATCTGGCGC AGATCGTGTG CCGTCTGCCG GAGACATACG CCGTGAATAC CGCGGAACTC 
TTTAGCGAGG ACGAAGACTG TGATGGCTAC ATTTCGGAGC CGACCACACT GAATACGGAT 
CGGCGTCGCA TCCAGGTGTC GCGCGCTGGC AACTCGTTAT CCTTTTCCGA AACGGAAATG 
GACTCGGACG TGGAGCTTGA CCTGAAGCTG GAGCGCAAGA CGGACAAGTC CACACAGTCG 
TCGCCGCCGA GTAACAGCAG GTCGGACATG CGCGGCAAGG AAGAGGCTAG ACATATGTCT 
AATTGGCATT GGGGTGTGAA GCACAAGGAT GAGACTGGCA GCGGAGCCAC TGAAGCCACA 
AAAACACAAA CCGGTCAGGA GCAGGAACTC CAGCAGGAGC AACAGGATCA GGGAGCTACG 
GCTGAGCAGC TGGTGGATAA AGACAAGGAG GAGAACAAAG AGAACAGAAG TAGCCCGAAT 
AACGAAACCA AAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727224.2
CDS	696..4973
Translation	MDVLEMLRASASGSYNTIFSDAWCQYVSKQITATVYMYFALVMMSLLFIAWFLYFKRMARLRLRDEIARSISTVTNSSGDMRGLRFRKRDKMLFYGRRMLRKMKNVSGQMYSSGKGYKRRAVMRFARRILQLRRDNMPLEMRTVEPPAEYLEETIEGSDRVPPDALYMLQSIRIFGHFEKPVFLRLCKHTQLLELMAGDYLFKITDPDDSVYIVQSGMINVYISNADGSTLSLKTVRKGESVTSLLSFIDVLSGNPSYYKTVTAKAIEKSVVIRLPMQAFEEVFQDNPDVMIRVIQVIMIRLQRVLFTALRNYLGLNAELVQNHMRYKSVSTMSGPINSQTSQSSRQAPNGPPMVISQMNLMQSAVSGTGSSGVSVTVTRPPSSPSRHSREEHTLSDPNPNPDGSFHGTTNLFTEVHGDAPNADLFHQQQQQHSVGNLSTRRSSITLMAPDGSHSCLQTPGVTTSIDMRLVQSSAVDSLRKELGLSEEDSHIIEPFVELRELEPNVTLITEGNADDVCVWFVMTGTLAVYQSNQDATRAKQDKSDMLIHFVHPGEIVGGLAMLTGEASAYTIRSRSITRIAFIRRAAIYQIMRQRPRIVLDLGNGVVRRLSPLVRQCDYALDWIFLESGRAVYRQDESSDSTYIVLSGRMRSVITHPGGKKEIVGEYGKGDLVGIVEMITETSRTTTVMAVRDSELAKLPEGLFNAIKLRYPIVVTKLISFLSHRFLGSMQTRSGSGAPGAPVEANPVTHKYSTVALVPITDEVPMTPFTYELYHSLCAIGPVLRLTSDVVRKQLGSNIFEAANEYRLTSWLAQQEDRNIITLYQCDSSLSAWTQRCMRQADVILIVGLGDRSHLVGKFEREIDRLAMRTQKELVLLYPEASNAKPANTLSWLNARPWVTKHHHVLCVKRIFTRKSQYRINDLYSRVLLSEPNMHSDFSRLARWLTGNSIGLVLGGGGARGAAHIGMLKAIQEAGIPVDMVGGVSIGALMGALWCSERNITTVTQKAREWSKKMTKWFLQLLDLTYPITSMFSGREFNKTIHDTFGDVSIEDLWIPYFTLTTDITASCHRIHTNGHLWRYCRASMSIAGVFPPFCDYRDGHLLLDGCYTNNVPADVMHNLGAAHIIAIDVGSQDDTDLTNYGDDLSGWWLLYKKWNPFTSPVKVPDLPDIQSRLAYVSCVRQLEEVKNSDYCEYIRPPIDKYKTLAFGSFDEIRDVGYVFGKNYFESMAKAGRLGRFNQWFNKEPPKRVNHASLNEYTFIDLAQIVCRLPETYAVNTAELFSEDEDCDGYISEPTTLNTDRRRIQVSRAGNSLSFSETEMDSDVELDLKLERKTDKSTQSSPPSNSRSDMRGKEEARHMSNWHWGVKHKDETGSGATEATKTQTGQEQELQQEQQDQGATAEQLVDKDKEENKENRSSPNNETKN

Target ORF information:

RefSeq Version	NM_167140.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster swiss cheese (sws), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167140.2

ORF Insert Sequence:

ATGGATGTCC TGGAAATGCT GCGCGCCAGC GCCAGCGGCA GCTACAACAC AATATTCTCG 
GACGCGTGGT GCCAATATGT CTCCAAGCAG ATCACAGCAA CGGTGTACAT GTATTTCGCC 
CTGGTAATGA TGAGCCTGCT CTTCATAGCC TGGTTCTTAT ACTTCAAACG AATGGCACGT 
TTGCGTTTGC GCGACGAGAT TGCCCGTTCC ATTAGCACCG TAACCAATTC ATCCGGCGAT 
ATGCGTGGCC TGCGTTTCCG GAAGCGTGAC AAGATGCTAT TCTACGGCCG TCGAATGCTG 
CGCAAAATGA AGAACGTGAG CGGTCAAATG TACAGCAGCG GTAAGGGCTA CAAGCGTCGT 
GCTGTCATGC GTTTCGCGAG AAGGATACTC CAGCTGAGGC GGGATAATAT GCCGCTAGAG 
ATGCGCACCG TGGAGCCGCC AGCCGAGTAT CTGGAGGAGA CAATCGAGGG CAGCGATAGA 
GTTCCGCCCG ATGCACTCTA TATGCTCCAG AGCATTCGCA TTTTTGGACA TTTCGAGAAG 
CCGGTCTTTT TGCGGCTCTG CAAGCACACC CAATTGCTGG AACTGATGGC CGGCGACTAT 
TTGTTTAAGA TAACCGATCC GGACGATAGC GTCTATATCG TTCAATCGGG CATGATCAAT 
GTGTACATCT CCAATGCCGA TGGCAGCACA CTATCATTGA AGACGGTCAG GAAGGGCGAG 
TCGGTGACAT CGCTGCTCAG CTTTATTGAT GTCCTGTCGG GGAATCCCAG CTACTATAAG 
ACGGTCACCG CCAAAGCCAT TGAGAAATCG GTGGTCATTC GTCTGCCCAT GCAGGCATTC 
GAAGAGGTGT TCCAGGACAA TCCGGACGTA ATGATTCGCG TCATCCAGGT GATTATGATC 
CGGCTACAAC GCGTACTGTT CACCGCTTTG CGCAACTACC TCGGTCTAAA TGCCGAACTG 
GTGCAGAATC ACATGCGCTA CAAGAGCGTA TCCACAATGA GTGGACCGAT TAACTCGCAG 
ACCTCGCAAT CCTCACGCCA GGCGCCCAAT GGGCCGCCCA TGGTGATCAG CCAGATGAAT 
CTGATGCAGT CCGCTGTTTC GGGAACGGGA TCATCGGGTG TCTCGGTGAC CGTAACTCGA 
CCACCATCCT CGCCATCGCG ACACTCGCGC GAGGAGCACA CGCTATCCGA TCCGAATCCA 
AATCCGGATG GTAGTTTTCA CGGCACTACC AATCTGTTCA CCGAGGTGCA TGGCGATGCG 
CCCAATGCAG ATCTATTCCA TCAGCAGCAG CAGCAGCATT CGGTGGGCAA CCTATCCACG 
CGTCGCAGTT CCATAACTCT GATGGCACCC GATGGTTCGC ACTCGTGTCT ACAGACACCC 
GGTGTGACAA CCTCGATAGA CATGCGCCTG GTGCAATCCT CAGCGGTGGA CAGCCTACGC 
AAGGAATTGG GTCTGTCCGA GGAGGATTCC CACATCATTG AGCCTTTTGT AGAGTTAAGG 
GAACTGGAGC CGAATGTCAC CCTGATTACG GAGGGCAATG CGGACGATGT TTGCGTTTGG 
TTTGTGATGA CGGGCACATT GGCTGTTTAC CAGAGCAATC AGGATGCCAC GCGGGCCAAG 
CAGGATAAGA GCGACATGCT CATTCACTTT GTGCATCCCG GCGAAATCGT TGGTGGACTG 
GCCATGCTAA CGGGAGAGGC CAGTGCGTAT ACCATTCGAT CGAGAAGCAT CACCAGAATA 
GCCTTCATCC GACGAGCAGC TATTTACCAG ATCATGCGAC AACGACCACG CATTGTTTTG 
GATCTGGGCA ACGGAGTAGT ACGCCGTCTA TCGCCTCTGG TGAGGCAGTG TGACTATGCC 
TTGGACTGGA TCTTTCTGGA ATCGGGCAGA GCTGTCTATC GGCAGGACGA GAGCAGCGAC 
AGCACATATA TTGTGCTTAG CGGGCGAATG CGTTCGGTTA TCACGCATCC TGGTGGCAAA 
AAGGAGATCG TCGGCGAATA CGGCAAGGGG GATCTTGTGG GCATCGTTGA AATGATCACG 
GAAACGTCGC GCACCACGAC GGTGATGGCT GTGCGCGACT CGGAGCTGGC CAAGCTGCCC 
GAGGGCCTGT TCAATGCCAT CAAGCTGCGC TACCCCATCG TGGTGACCAA GCTGATTAGC 
TTCCTTAGCC ACCGCTTCCT CGGCTCGATG CAGACGCGCT CGGGCAGCGG GGCGCCGGGC 
GCGCCCGTCG AGGCCAATCC CGTCACGCAC AAGTACTCCA CGGTGGCCCT GGTGCCCATC 
ACCGACGAGG TGCCGATGAC GCCCTTCACC TACGAGCTCT ACCACTCGCT CTGTGCCATA 
GGACCCGTTC TCCGTTTGAC CTCCGATGTG GTGCGCAAGC AGCTGGGCTC CAACATCTTT 
GAGGCAGCCA ACGAGTATCG TCTAACCTCG TGGCTGGCGC AGCAGGAGGA CCGAAACATC 
ATCACCCTCT ATCAGTGTGA CAGTTCGCTG AGCGCGTGGA CACAGCGCTG CATGCGACAG 
GCTGACGTTA TCCTGATCGT GGGCCTCGGC GATCGATCAC ACCTGGTCGG AAAGTTCGAA 
CGCGAGATCG ACCGGCTGGC GATGCGAACG CAAAAGGAGC TCGTCCTGCT ATATCCGGAG 
GCCAGCAATG CCAAGCCGGC CAACACGCTT AGTTGGCTAA ATGCCCGACC CTGGGTAACA 
AAGCACCACC ATGTGCTCTG CGTCAAGCGG ATCTTCACGC GCAAAAGTCA ATATCGCATT 
AATGATCTCT ATAGTCGCGT CCTGCTGTCC GAGCCAAACA TGCATTCCGA CTTCTCGCGT 
TTGGCGCGCT GGCTTACGGG CAACTCGATT GGCCTGGTGC TGGGCGGCGG AGGAGCGCGT 
GGAGCTGCCC ACATTGGTAT GCTGAAGGCC ATCCAGGAGG CGGGCATACC TGTTGACATG 
GTGGGCGGCG TCAGCATCGG TGCCTTGATG GGAGCTCTCT GGTGCTCGGA GCGTAATATC 
ACCACGGTAA CGCAGAAGGC GCGCGAGTGG TCAAAGAAAA TGACGAAATG GTTCCTACAA 
CTACTTGACC TCACCTACCC GATCACATCG ATGTTCTCCG GACGAGAGTT CAACAAGACG 
ATCCACGACA CTTTCGGGGA CGTGAGCATC GAGGACCTGT GGATACCCTA CTTCACCCTC 
ACCACCGACA TAACCGCCAG TTGCCATCGC ATTCACACCA ATGGCCACCT GTGGCGGTAC 
TGCCGCGCCA GCATGTCGAT TGCCGGCGTC TTCCCACCAT TTTGTGATTA CCGCGACGGC 
CACCTCCTGC TCGATGGCTG CTACACGAAC AACGTGCCAG CCGATGTGAT GCATAATCTT 
GGAGCTGCTC ACATAATCGC CATCGATGTG GGATCCCAGG ATGACACTGA CCTGACCAAC 
TATGGCGACG ACCTAAGCGG TTGGTGGCTG CTCTACAAGA AATGGAATCC CTTCACATCG 
CCTGTGAAGG TGCCCGACTT GCCGGACATC CAATCCCGAC TAGCCTATGT GTCCTGCGTA 
CGCCAACTAG AGGAGGTCAA AAACTCCGAC TACTGCGAGT ATATCCGACC GCCGATCGAT 
AAGTACAAGA CTCTAGCCTT CGGCAGCTTC GACGAAATTC GAGACGTGGG CTATGTGTTT 
GGCAAAAACT ACTTTGAGAG CATGGCCAAG GCTGGTCGAT TGGGCCGCTT CAATCAGTGG 
TTCAACAAAG AGCCACCCAA GCGGGTTAAC CATGCCTCCC TCAACGAGTA CACGTTCATC 
GATCTGGCGC AGATCGTGTG CCGTCTGCCG GAGACATACG CCGTGAATAC CGCGGAACTC 
TTTAGCGAGG ACGAAGACTG TGATGGCTAC ATTTCGGAGC CGACCACACT GAATACGGAT 
CGGCGTCGCA TCCAGGTGTC GCGCGCTGGC AACTCGTTAT CCTTTTCCGA AACGGAAATG 
GACTCGGACG TGGAGCTTGA CCTGAAGCTG GAGCGCAAGA CGGACAAGTC CACACAGTCG 
TCGCCGCCGA GTAACAGCAG GTCGGACATG CGCGGCAAGG AAGAGGCTAG ACATATGTCT 
AATTGGCATT GGGGTGTGAA GCACAAGGAT GAGACTGGCA GCGGAGCCAC TGAAGCCACA 
AAAACACAAA CCGGTCAGGA GCAGGAACTC CAGCAGGAGC AACAGGATCA GGGAGCTACG 
GCTGAGCAGC TGGTGGATAA AGACAAGGAG GAGAACAAAG AGAACAGAAG TAGCCCGAAT 
AACGAAACCA AAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sws ( NM_078520.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511075.3 Drosophila melanogaster swiss cheese (sws), transcript variant A, mRNA.

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CloneID	OFa01062
Clone ID Related Accession (Same CDS sequence)	NM_078520.3
Accession Version	NM_078520.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	swiss cheese, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_078520.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGTCC TGGAAATGCT GCGCGCCAGC GCCAGCGGCA GCTACAACAC AATATTCTCG 
GACGCGTGGT GCCAATATGT CTCCAAGCAG ATCACAGCAA CGGTGTACAT GTATTTCGCC 
CTGGTAATGA TGAGCCTGCT CTTCATAGCC TGGTTCTTAT ACTTCAAACG AATGGCACGT 
TTGCGTTTGC GCGACGAGAT TGCCCGTTCC ATTAGCACCG TAACCAATTC ATCCGGCGAT 
ATGCGTGGCC TGCGTTTCCG GAAGCGTGAC AAGATGCTAT TCTACGGCCG TCGAATGCTG 
CGCAAAATGA AGAACGTGAG CGGTCAAATG TACAGCAGCG GTAAGGGCTA CAAGCGTCGT 
GCTGTCATGC GTTTCGCGAG AAGGATACTC CAGCTGAGGC GGGATAATAT GCCGCTAGAG 
ATGCGCACCG TGGAGCCGCC AGCCGAGTAT CTGGAGGAGA CAATCGAGGG CAGCGATAGA 
GTTCCGCCCG ATGCACTCTA TATGCTCCAG AGCATTCGCA TTTTTGGACA TTTCGAGAAG 
CCGGTCTTTT TGCGGCTCTG CAAGCACACC CAATTGCTGG AACTGATGGC CGGCGACTAT 
TTGTTTAAGA TAACCGATCC GGACGATAGC GTCTATATCG TTCAATCGGG CATGATCAAT 
GTGTACATCT CCAATGCCGA TGGCAGCACA CTATCATTGA AGACGGTCAG GAAGGGCGAG 
TCGGTGACAT CGCTGCTCAG CTTTATTGAT GTCCTGTCGG GGAATCCCAG CTACTATAAG 
ACGGTCACCG CCAAAGCCAT TGAGAAATCG GTGGTCATTC GTCTGCCCAT GCAGGCATTC 
GAAGAGGTGT TCCAGGACAA TCCGGACGTA ATGATTCGCG TCATCCAGGT GATTATGATC 
CGGCTACAAC GCGTACTGTT CACCGCTTTG CGCAACTACC TCGGTCTAAA TGCCGAACTG 
GTGCAGAATC ACATGCGCTA CAAGAGCGTA TCCACAATGA GTGGACCGAT TAACTCGCAG 
ACCTCGCAAT CCTCACGCCA GGCGCCCAAT GGGCCGCCCA TGGTGATCAG CCAGATGAAT 
CTGATGCAGT CCGCTGTTTC GGGAACGGGA TCATCGGGTG TCTCGGTGAC CGTAACTCGA 
CCACCATCCT CGCCATCGCG ACACTCGCGC GAGGAGCACA CGCTATCCGA TCCGAATCCA 
AATCCGGATG GTAGTTTTCA CGGCACTACC AATCTGTTCA CCGAGGTGCA TGGCGATGCG 
CCCAATGCAG ATCTATTCCA TCAGCAGCAG CAGCAGCATT CGGTGGGCAA CCTATCCACG 
CGTCGCAGTT CCATAACTCT GATGGCACCC GATGGTTCGC ACTCGTGTCT ACAGACACCC 
GGTGTGACAA CCTCGATAGA CATGCGCCTG GTGCAATCCT CAGCGGTGGA CAGCCTACGC 
AAGGAATTGG GTCTGTCCGA GGAGGATTCC CACATCATTG AGCCTTTTGT AGAGTTAAGG 
GAACTGGAGC CGAATGTCAC CCTGATTACG GAGGGCAATG CGGACGATGT TTGCGTTTGG 
TTTGTGATGA CGGGCACATT GGCTGTTTAC CAGAGCAATC AGGATGCCAC GCGGGCCAAG 
CAGGATAAGA GCGACATGCT CATTCACTTT GTGCATCCCG GCGAAATCGT TGGTGGACTG 
GCCATGCTAA CGGGAGAGGC CAGTGCGTAT ACCATTCGAT CGAGAAGCAT CACCAGAATA 
GCCTTCATCC GACGAGCAGC TATTTACCAG ATCATGCGAC AACGACCACG CATTGTTTTG 
GATCTGGGCA ACGGAGTAGT ACGCCGTCTA TCGCCTCTGG TGAGGCAGTG TGACTATGCC 
TTGGACTGGA TCTTTCTGGA ATCGGGCAGA GCTGTCTATC GGCAGGACGA GAGCAGCGAC 
AGCACATATA TTGTGCTTAG CGGGCGAATG CGTTCGGTTA TCACGCATCC TGGTGGCAAA 
AAGGAGATCG TCGGCGAATA CGGCAAGGGG GATCTTGTGG GCATCGTTGA AATGATCACG 
GAAACGTCGC GCACCACGAC GGTGATGGCT GTGCGCGACT CGGAGCTGGC CAAGCTGCCC 
GAGGGCCTGT TCAATGCCAT CAAGCTGCGC TACCCCATCG TGGTGACCAA GCTGATTAGC 
TTCCTTAGCC ACCGCTTCCT CGGCTCGATG CAGACGCGCT CGGGCAGCGG GGCGCCGGGC 
GCGCCCGTCG AGGCCAATCC CGTCACGCAC AAGTACTCCA CGGTGGCCCT GGTGCCCATC 
ACCGACGAGG TGCCGATGAC GCCCTTCACC TACGAGCTCT ACCACTCGCT CTGTGCCATA 
GGACCCGTTC TCCGTTTGAC CTCCGATGTG GTGCGCAAGC AGCTGGGCTC CAACATCTTT 
GAGGCAGCCA ACGAGTATCG TCTAACCTCG TGGCTGGCGC AGCAGGAGGA CCGAAACATC 
ATCACCCTCT ATCAGTGTGA CAGTTCGCTG AGCGCGTGGA CACAGCGCTG CATGCGACAG 
GCTGACGTTA TCCTGATCGT GGGCCTCGGC GATCGATCAC ACCTGGTCGG AAAGTTCGAA 
CGCGAGATCG ACCGGCTGGC GATGCGAACG CAAAAGGAGC TCGTCCTGCT ATATCCGGAG 
GCCAGCAATG CCAAGCCGGC CAACACGCTT AGTTGGCTAA ATGCCCGACC CTGGGTAACA 
AAGCACCACC ATGTGCTCTG CGTCAAGCGG ATCTTCACGC GCAAAAGTCA ATATCGCATT 
AATGATCTCT ATAGTCGCGT CCTGCTGTCC GAGCCAAACA TGCATTCCGA CTTCTCGCGT 
TTGGCGCGCT GGCTTACGGG CAACTCGATT GGCCTGGTGC TGGGCGGCGG AGGAGCGCGT 
GGAGCTGCCC ACATTGGTAT GCTGAAGGCC ATCCAGGAGG CGGGCATACC TGTTGACATG 
GTGGGCGGCG TCAGCATCGG TGCCTTGATG GGAGCTCTCT GGTGCTCGGA GCGTAATATC 
ACCACGGTAA CGCAGAAGGC GCGCGAGTGG TCAAAGAAAA TGACGAAATG GTTCCTACAA 
CTACTTGACC TCACCTACCC GATCACATCG ATGTTCTCCG GACGAGAGTT CAACAAGACG 
ATCCACGACA CTTTCGGGGA CGTGAGCATC GAGGACCTGT GGATACCCTA CTTCACCCTC 
ACCACCGACA TAACCGCCAG TTGCCATCGC ATTCACACCA ATGGATCTCT GTGGCGTTAC 
GTACGCTCCT CCATGTCGCT CAGTGGCTAC ATGCCGCCGT TATGCGATCC TAAAGATGGG 
CACCTCTTGC TGGATGGCGG CTATGTGAAT AATCTGCCAG CCGATGTGAT GCATAATCTT 
GGAGCTGCTC ACATAATCGC CATCGATGTG GGATCCCAGG ATGACACTGA CCTGACCAAC 
TATGGCGACG ACCTAAGCGG TTGGTGGCTG CTCTACAAGA AATGGAATCC CTTCACATCG 
CCTGTGAAGG TGCCCGACTT GCCGGACATC CAATCCCGAC TAGCCTATGT GTCCTGCGTA 
CGCCAACTAG AGGAGGTCAA AAACTCCGAC TACTGCGAGT ATATCCGACC GCCGATCGAT 
AAGTACAAGA CTCTAGCCTT CGGCAGCTTC GACGAAATTC GAGACGTGGG CTATGTGTTT 
GGCAAAAACT ACTTTGAGAG CATGGCCAAG GCTGGTCGAT TGGGCCGCTT CAATCAGTGG 
TTCAACAAAG AGCCACCCAA GCGGGTTAAC CATGCCTCCC TCAACGAGTA CACGTTCATC 
GATCTGGCGC AGATCGTGTG CCGTCTGCCG GAGACATACG CCGTGAATAC CGCGGAACTC 
TTTAGCGAGG ACGAAGACTG TGATGGCTAC ATTTCGGAGC CGACCACACT GAATACGGAT 
CGGCGTCGCA TCCAGGTGTC GCGCGCTGGC AACTCGTTAT CCTTTTCCGA AACGGAAATG 
GACTCGGACG TGGAGCTTGA CCTGAAGCTG GAGCGCAAGA CGGACAAGTC CACACAGTCG 
TCGCCGCCGA GTAACAGCAG GTCGGACATG CGCGGCAAGG AAGAGGCTAG ACATATGTCT 
AATTGGCATT GGGGTGTGAA GCACAAGGAT GAGACTGGCA GCGGAGCCAC TGAAGCCACA 
AAAACACAAA CCGGTCAGGA GCAGGAACTC CAGCAGGAGC AACAGGATCA GGGAGCTACG 
GCTGAGCAGC TGGTGGATAA AGACAAGGAG GAGAACAAAG AGAACAGAAG TAGCCCGAAT 
AACGAAACCA AAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511075.3
CDS	696..4973
Translation	MDVLEMLRASASGSYNTIFSDAWCQYVSKQITATVYMYFALVMMSLLFIAWFLYFKRMARLRLRDEIARSISTVTNSSGDMRGLRFRKRDKMLFYGRRMLRKMKNVSGQMYSSGKGYKRRAVMRFARRILQLRRDNMPLEMRTVEPPAEYLEETIEGSDRVPPDALYMLQSIRIFGHFEKPVFLRLCKHTQLLELMAGDYLFKITDPDDSVYIVQSGMINVYISNADGSTLSLKTVRKGESVTSLLSFIDVLSGNPSYYKTVTAKAIEKSVVIRLPMQAFEEVFQDNPDVMIRVIQVIMIRLQRVLFTALRNYLGLNAELVQNHMRYKSVSTMSGPINSQTSQSSRQAPNGPPMVISQMNLMQSAVSGTGSSGVSVTVTRPPSSPSRHSREEHTLSDPNPNPDGSFHGTTNLFTEVHGDAPNADLFHQQQQQHSVGNLSTRRSSITLMAPDGSHSCLQTPGVTTSIDMRLVQSSAVDSLRKELGLSEEDSHIIEPFVELRELEPNVTLITEGNADDVCVWFVMTGTLAVYQSNQDATRAKQDKSDMLIHFVHPGEIVGGLAMLTGEASAYTIRSRSITRIAFIRRAAIYQIMRQRPRIVLDLGNGVVRRLSPLVRQCDYALDWIFLESGRAVYRQDESSDSTYIVLSGRMRSVITHPGGKKEIVGEYGKGDLVGIVEMITETSRTTTVMAVRDSELAKLPEGLFNAIKLRYPIVVTKLISFLSHRFLGSMQTRSGSGAPGAPVEANPVTHKYSTVALVPITDEVPMTPFTYELYHSLCAIGPVLRLTSDVVRKQLGSNIFEAANEYRLTSWLAQQEDRNIITLYQCDSSLSAWTQRCMRQADVILIVGLGDRSHLVGKFEREIDRLAMRTQKELVLLYPEASNAKPANTLSWLNARPWVTKHHHVLCVKRIFTRKSQYRINDLYSRVLLSEPNMHSDFSRLARWLTGNSIGLVLGGGGARGAAHIGMLKAIQEAGIPVDMVGGVSIGALMGALWCSERNITTVTQKAREWSKKMTKWFLQLLDLTYPITSMFSGREFNKTIHDTFGDVSIEDLWIPYFTLTTDITASCHRIHTNGSLWRYVRSSMSLSGYMPPLCDPKDGHLLLDGGYVNNLPADVMHNLGAAHIIAIDVGSQDDTDLTNYGDDLSGWWLLYKKWNPFTSPVKVPDLPDIQSRLAYVSCVRQLEEVKNSDYCEYIRPPIDKYKTLAFGSFDEIRDVGYVFGKNYFESMAKAGRLGRFNQWFNKEPPKRVNHASLNEYTFIDLAQIVCRLPETYAVNTAELFSEDEDCDGYISEPTTLNTDRRRIQVSRAGNSLSFSETEMDSDVELDLKLERKTDKSTQSSPPSNSRSDMRGKEEARHMSNWHWGVKHKDETGSGATEATKTQTGQEQELQQEQQDQGATAEQLVDKDKEENKENRSSPNNETKN

Target ORF information:

RefSeq Version	NM_078520.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster swiss cheese (sws), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078520.3

ORF Insert Sequence:

ATGGATGTCC TGGAAATGCT GCGCGCCAGC GCCAGCGGCA GCTACAACAC AATATTCTCG 
GACGCGTGGT GCCAATATGT CTCCAAGCAG ATCACAGCAA CGGTGTACAT GTATTTCGCC 
CTGGTAATGA TGAGCCTGCT CTTCATAGCC TGGTTCTTAT ACTTCAAACG AATGGCACGT 
TTGCGTTTGC GCGACGAGAT TGCCCGTTCC ATTAGCACCG TAACCAATTC ATCCGGCGAT 
ATGCGTGGCC TGCGTTTCCG GAAGCGTGAC AAGATGCTAT TCTACGGCCG TCGAATGCTG 
CGCAAAATGA AGAACGTGAG CGGTCAAATG TACAGCAGCG GTAAGGGCTA CAAGCGTCGT 
GCTGTCATGC GTTTCGCGAG AAGGATACTC CAGCTGAGGC GGGATAATAT GCCGCTAGAG 
ATGCGCACCG TGGAGCCGCC AGCCGAGTAT CTGGAGGAGA CAATCGAGGG CAGCGATAGA 
GTTCCGCCCG ATGCACTCTA TATGCTCCAG AGCATTCGCA TTTTTGGACA TTTCGAGAAG 
CCGGTCTTTT TGCGGCTCTG CAAGCACACC CAATTGCTGG AACTGATGGC CGGCGACTAT 
TTGTTTAAGA TAACCGATCC GGACGATAGC GTCTATATCG TTCAATCGGG CATGATCAAT 
GTGTACATCT CCAATGCCGA TGGCAGCACA CTATCATTGA AGACGGTCAG GAAGGGCGAG 
TCGGTGACAT CGCTGCTCAG CTTTATTGAT GTCCTGTCGG GGAATCCCAG CTACTATAAG 
ACGGTCACCG CCAAAGCCAT TGAGAAATCG GTGGTCATTC GTCTGCCCAT GCAGGCATTC 
GAAGAGGTGT TCCAGGACAA TCCGGACGTA ATGATTCGCG TCATCCAGGT GATTATGATC 
CGGCTACAAC GCGTACTGTT CACCGCTTTG CGCAACTACC TCGGTCTAAA TGCCGAACTG 
GTGCAGAATC ACATGCGCTA CAAGAGCGTA TCCACAATGA GTGGACCGAT TAACTCGCAG 
ACCTCGCAAT CCTCACGCCA GGCGCCCAAT GGGCCGCCCA TGGTGATCAG CCAGATGAAT 
CTGATGCAGT CCGCTGTTTC GGGAACGGGA TCATCGGGTG TCTCGGTGAC CGTAACTCGA 
CCACCATCCT CGCCATCGCG ACACTCGCGC GAGGAGCACA CGCTATCCGA TCCGAATCCA 
AATCCGGATG GTAGTTTTCA CGGCACTACC AATCTGTTCA CCGAGGTGCA TGGCGATGCG 
CCCAATGCAG ATCTATTCCA TCAGCAGCAG CAGCAGCATT CGGTGGGCAA CCTATCCACG 
CGTCGCAGTT CCATAACTCT GATGGCACCC GATGGTTCGC ACTCGTGTCT ACAGACACCC 
GGTGTGACAA CCTCGATAGA CATGCGCCTG GTGCAATCCT CAGCGGTGGA CAGCCTACGC 
AAGGAATTGG GTCTGTCCGA GGAGGATTCC CACATCATTG AGCCTTTTGT AGAGTTAAGG 
GAACTGGAGC CGAATGTCAC CCTGATTACG GAGGGCAATG CGGACGATGT TTGCGTTTGG 
TTTGTGATGA CGGGCACATT GGCTGTTTAC CAGAGCAATC AGGATGCCAC GCGGGCCAAG 
CAGGATAAGA GCGACATGCT CATTCACTTT GTGCATCCCG GCGAAATCGT TGGTGGACTG 
GCCATGCTAA CGGGAGAGGC CAGTGCGTAT ACCATTCGAT CGAGAAGCAT CACCAGAATA 
GCCTTCATCC GACGAGCAGC TATTTACCAG ATCATGCGAC AACGACCACG CATTGTTTTG 
GATCTGGGCA ACGGAGTAGT ACGCCGTCTA TCGCCTCTGG TGAGGCAGTG TGACTATGCC 
TTGGACTGGA TCTTTCTGGA ATCGGGCAGA GCTGTCTATC GGCAGGACGA GAGCAGCGAC 
AGCACATATA TTGTGCTTAG CGGGCGAATG CGTTCGGTTA TCACGCATCC TGGTGGCAAA 
AAGGAGATCG TCGGCGAATA CGGCAAGGGG GATCTTGTGG GCATCGTTGA AATGATCACG 
GAAACGTCGC GCACCACGAC GGTGATGGCT GTGCGCGACT CGGAGCTGGC CAAGCTGCCC 
GAGGGCCTGT TCAATGCCAT CAAGCTGCGC TACCCCATCG TGGTGACCAA GCTGATTAGC 
TTCCTTAGCC ACCGCTTCCT CGGCTCGATG CAGACGCGCT CGGGCAGCGG GGCGCCGGGC 
GCGCCCGTCG AGGCCAATCC CGTCACGCAC AAGTACTCCA CGGTGGCCCT GGTGCCCATC 
ACCGACGAGG TGCCGATGAC GCCCTTCACC TACGAGCTCT ACCACTCGCT CTGTGCCATA 
GGACCCGTTC TCCGTTTGAC CTCCGATGTG GTGCGCAAGC AGCTGGGCTC CAACATCTTT 
GAGGCAGCCA ACGAGTATCG TCTAACCTCG TGGCTGGCGC AGCAGGAGGA CCGAAACATC 
ATCACCCTCT ATCAGTGTGA CAGTTCGCTG AGCGCGTGGA CACAGCGCTG CATGCGACAG 
GCTGACGTTA TCCTGATCGT GGGCCTCGGC GATCGATCAC ACCTGGTCGG AAAGTTCGAA 
CGCGAGATCG ACCGGCTGGC GATGCGAACG CAAAAGGAGC TCGTCCTGCT ATATCCGGAG 
GCCAGCAATG CCAAGCCGGC CAACACGCTT AGTTGGCTAA ATGCCCGACC CTGGGTAACA 
AAGCACCACC ATGTGCTCTG CGTCAAGCGG ATCTTCACGC GCAAAAGTCA ATATCGCATT 
AATGATCTCT ATAGTCGCGT CCTGCTGTCC GAGCCAAACA TGCATTCCGA CTTCTCGCGT 
TTGGCGCGCT GGCTTACGGG CAACTCGATT GGCCTGGTGC TGGGCGGCGG AGGAGCGCGT 
GGAGCTGCCC ACATTGGTAT GCTGAAGGCC ATCCAGGAGG CGGGCATACC TGTTGACATG 
GTGGGCGGCG TCAGCATCGG TGCCTTGATG GGAGCTCTCT GGTGCTCGGA GCGTAATATC 
ACCACGGTAA CGCAGAAGGC GCGCGAGTGG TCAAAGAAAA TGACGAAATG GTTCCTACAA 
CTACTTGACC TCACCTACCC GATCACATCG ATGTTCTCCG GACGAGAGTT CAACAAGACG 
ATCCACGACA CTTTCGGGGA CGTGAGCATC GAGGACCTGT GGATACCCTA CTTCACCCTC 
ACCACCGACA TAACCGCCAG TTGCCATCGC ATTCACACCA ATGGATCTCT GTGGCGTTAC 
GTACGCTCCT CCATGTCGCT CAGTGGCTAC ATGCCGCCGT TATGCGATCC TAAAGATGGG 
CACCTCTTGC TGGATGGCGG CTATGTGAAT AATCTGCCAG CCGATGTGAT GCATAATCTT 
GGAGCTGCTC ACATAATCGC CATCGATGTG GGATCCCAGG ATGACACTGA CCTGACCAAC 
TATGGCGACG ACCTAAGCGG TTGGTGGCTG CTCTACAAGA AATGGAATCC CTTCACATCG 
CCTGTGAAGG TGCCCGACTT GCCGGACATC CAATCCCGAC TAGCCTATGT GTCCTGCGTA 
CGCCAACTAG AGGAGGTCAA AAACTCCGAC TACTGCGAGT ATATCCGACC GCCGATCGAT 
AAGTACAAGA CTCTAGCCTT CGGCAGCTTC GACGAAATTC GAGACGTGGG CTATGTGTTT 
GGCAAAAACT ACTTTGAGAG CATGGCCAAG GCTGGTCGAT TGGGCCGCTT CAATCAGTGG 
TTCAACAAAG AGCCACCCAA GCGGGTTAAC CATGCCTCCC TCAACGAGTA CACGTTCATC 
GATCTGGCGC AGATCGTGTG CCGTCTGCCG GAGACATACG CCGTGAATAC CGCGGAACTC 
TTTAGCGAGG ACGAAGACTG TGATGGCTAC ATTTCGGAGC CGACCACACT GAATACGGAT 
CGGCGTCGCA TCCAGGTGTC GCGCGCTGGC AACTCGTTAT CCTTTTCCGA AACGGAAATG 
GACTCGGACG TGGAGCTTGA CCTGAAGCTG GAGCGCAAGA CGGACAAGTC CACACAGTCG 
TCGCCGCCGA GTAACAGCAG GTCGGACATG CGCGGCAAGG AAGAGGCTAG ACATATGTCT 
AATTGGCATT GGGGTGTGAA GCACAAGGAT GAGACTGGCA GCGGAGCCAC TGAAGCCACA 
AAAACACAAA CCGGTCAGGA GCAGGAACTC CAGCAGGAGC AACAGGATCA GGGAGCTACG 
GCTGAGCAGC TGGTGGATAA AGACAAGGAG GAGAACAAAG AGAACAGAAG TAGCCCGAAT 
AACGAAACCA AAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sws cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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