PNPLA7 cDNA ORF clone, Bos taurus(cattle)

The following PNPLA7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNPLA7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221032 XM_024999698.1
Latest version!
Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221033 XM_010810587.3
Latest version!
Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221034 XM_010810588.3
Latest version!
Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa53358 XM_010810580.2
Latest version!
Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa53358 XM_015465574.1
Latest version!
Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa53359 XM_015465575.1
Latest version!
Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa53359 XM_010810587.2
Latest version!
Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa53360 XM_015465576.1
Latest version!
Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa53360 XM_010810588.2
Latest version!
Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa221032
    Clone ID Related Accession (Same CDS sequence) XM_024999698.1
    Accession Version XM_024999698.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product patatin-like phospholipase domain-containing protein 7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC CCTTCGCTGT TAGATGGCCG GGTGGCGCTT
    CTCCACGTCC CAGGGGGCAC GGTGGTGTTG AGGCAGGGAG ACCAGGACAC GAGCATCCTC
    TTCGTGGTCT CGGGGCTGCT GCATGTGTAC CAGCGGAAGA TTGACAGCGA GGAGGACACG
    TTCCTGTTCA TGGCACGGCC CGGTGAGATG GTGGGCCAGC TGGCGGTGCT CACGGGCGAG
    CCCCTCATCT TCACCATCAG GGCCAACCGG GACTGCAGCT TCCTGTCCAT CTCCAAGGCG
    CACTTCTACG ACATCATGCG GAGGCAGCCA GCCGTGGTCC TGAGCGTGGC GCACACCGTG
    GTGAGGCGGG TGTCCTCCTT CGTGCGGCAG ATCGACTTCG CCCTGGACTG GATGGAGGTG
    GAGGCCGGCC GCGCCATATA CAGGCAGGGG GACAAGTCCG ACTGCACCTA CATCGTGCTC
    AGCGGCCGCC TGCGCGCTGT GATCCAGAAG GACGACGGCA AGAAGCGCCT GGCGGGCGAG
    TACGGCCGCG GAGACCTCGT GGGCGTGGTG GAGACACTCA CCCACCAGGC CAGGGCAACC
    ACAGTGCATG CTATTCGGGA CTCAGAGCTG GCCAAGCTTC CCGAAGGAGC CCTGAACTCC
    ATCAAGCGCC AGTACCCACA GGTTGTGACT CGACTGATTC GCCTCTTGGG CGAGAAGATC
    CTGGGCAGCC TCCAGCAGGG AGCTGCGGCA GGTCACCAGT TTGGGTTCCA TACTGTGGGC
    AGCAAGTGGG ACTCGGGGAA CGCCGCCAGC AACCTCTCCA CAGTGGCCGT CATGCCAGTG
    TCGGAGGACG TGCCCCTCAC CGCCTTCACC CTGGAGCTCA AGCACGCCCT CAGCGCTATT
    GGCCCTGCCC TGCTGCTGAC CAGTGACAGC ATAAAACAGC GCCTTGGCTC TGCTGCTCTG
    GACAGTATCC ACGAGTACCG GCTGTCCAGC TGGCTGGGGC AGCAGGAGGA CATCCACCGG
    ATCGTGCTCT ACCAGGCGGA CAGCACACTC ACGCCCTGGA CCCAGCGCTG CATCCGGCAG
    GCAGACTGCA TCCTCATCGT GGGCCTGGGC GAGCAGGAGC CCACGGTTGG CGAGCTGGAG
    CGGATGCTGG AGACTGCGGC CGTGCGTGCC CAGAAGCAGC TGGTGCTGCT GCACCGTCAG
    GACGGGCCCG CGCCGGCCCG CACCGCGGAG TGGCTCAACA TGCGGGGCTG GTGTTCTGGC
    CACCTGCACC TCTGCTGCCC TCGCCGCGTC TTCTCCAGGA GGAGCCTGCC CAAGCTGGTG
    GAGCTGTACG AGCGGGTTTG CCAGAGGCCC CCGGACCGGC ACTCGGACTT CTCCCGCCTG
    GCAAGAGTGC TGACCGGCAA TGCCATCGCC TTGGTCCTTG GGGGAGGGGG AGCAAGAGGC
    TGCGCGCAGG TGGGCATCAT CCGGGCTCTG ACGGAGTACG GCATCCCCGT GGACATGGTT
    GGAGGGACGT CCATCGGGGC CTTCATGGGC GCCCTGTACT CGGAGGAACG CAGCTACAGC
    CAGATCCGCA TCCGGGCCAA GCAGTGGGCG GAGGACATGA TGTCGATGGT GAAGACCATG
    CTGGACCTGA CCTACCCCAT CACTTCCATG TTCTCGGGGG CTGGCTTCAA CAGCAGCATC
    TCTAGCGTCT TCAAGGACAG GCAGATCGAG GACCTGTGGA TCCCATACTT CGCCATCACC
    ACGGACATCT CCGCATCTGC CATGCGGGTC CACACGGACG GATCACTGTG GAGGTACGTG
    CGTGCCAGCA TGTCCCTGTC GGGCTACATG CCCCCACTCT GCGACCCCAA AGATGGGCAC
    CTGCTGATGG ATGGGGGCTA CATCAACAAC CTCCCAGCGG ATGTGGCCAG GTCAATGGGG
    GCAAAGGTGG TTATCGCCAT TGACGTGGGC AGCCGGGATG AGACAGACCT CACCAACTAC
    GGGGATGCGC TCTCGGGATG GTGGCTCCTG TGGAAGCGCT GGAATCCCTT GGCCACGAAG
    GTCAAGGTGC TGAACATGGC TGAGATCCAG ACACGCCTGG CCTACGTGTG CTGTGTCCGG
    CAGCTGGAGA TGGTGAAGAG CAGCGACTAC TGCGAGTACC TGCGCCCTCC GATTGACAGC
    TATGGCACCC TGGAGTTCAG CAAGTTTGAT GAGATTTGTG AGGTGGGGTA CCAGCATGGC
    CGGACAGTGT TTGACATCTG GGGTCGAAGC GGTGTGCTGG AGAAGATGCT GCAGGACCGG
    CAGGGGACAA GCAAGATGAA GGCGTGCGAC GTCCTCACCT GCCCCAACGC CTCCTTCACG
    GACCTTGCTG AGATTGTATC ACGTATTGAG CCTGCCAAGG TGGCTGCAGG GGACGATGAA
    TCTGACTACC AGACTGAGTA TGAGGAGGTG CTGCTGGGAG GCCCCAAGGA CGCGTACGTT
    GACTTCCAAA GCGCCCCAGC CGACCGGGGC TCCGACTCGG AGAATGAGCC CTCGCTTCGG
    CATCAACACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855466.1
    CDS86..4057
    Translation

    Target ORF information:

    RefSeq Version XM_024999698.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999698.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC CCTTCGCTGT TAGATGGCCG GGTGGCGCTT
    CTCCACGTCC CAGGGGGCAC GGTGGTGTTG AGGCAGGGAG ACCAGGACAC GAGCATCCTC
    TTCGTGGTCT CGGGGCTGCT GCATGTGTAC CAGCGGAAGA TTGACAGCGA GGAGGACACG
    TTCCTGTTCA TGGCACGGCC CGGTGAGATG GTGGGCCAGC TGGCGGTGCT CACGGGCGAG
    CCCCTCATCT TCACCATCAG GGCCAACCGG GACTGCAGCT TCCTGTCCAT CTCCAAGGCG
    CACTTCTACG ACATCATGCG GAGGCAGCCA GCCGTGGTCC TGAGCGTGGC GCACACCGTG
    GTGAGGCGGG TGTCCTCCTT CGTGCGGCAG ATCGACTTCG CCCTGGACTG GATGGAGGTG
    GAGGCCGGCC GCGCCATATA CAGGCAGGGG GACAAGTCCG ACTGCACCTA CATCGTGCTC
    AGCGGCCGCC TGCGCGCTGT GATCCAGAAG GACGACGGCA AGAAGCGCCT GGCGGGCGAG
    TACGGCCGCG GAGACCTCGT GGGCGTGGTG GAGACACTCA CCCACCAGGC CAGGGCAACC
    ACAGTGCATG CTATTCGGGA CTCAGAGCTG GCCAAGCTTC CCGAAGGAGC CCTGAACTCC
    ATCAAGCGCC AGTACCCACA GGTTGTGACT CGACTGATTC GCCTCTTGGG CGAGAAGATC
    CTGGGCAGCC TCCAGCAGGG AGCTGCGGCA GGTCACCAGT TTGGGTTCCA TACTGTGGGC
    AGCAAGTGGG ACTCGGGGAA CGCCGCCAGC AACCTCTCCA CAGTGGCCGT CATGCCAGTG
    TCGGAGGACG TGCCCCTCAC CGCCTTCACC CTGGAGCTCA AGCACGCCCT CAGCGCTATT
    GGCCCTGCCC TGCTGCTGAC CAGTGACAGC ATAAAACAGC GCCTTGGCTC TGCTGCTCTG
    GACAGTATCC ACGAGTACCG GCTGTCCAGC TGGCTGGGGC AGCAGGAGGA CATCCACCGG
    ATCGTGCTCT ACCAGGCGGA CAGCACACTC ACGCCCTGGA CCCAGCGCTG CATCCGGCAG
    GCAGACTGCA TCCTCATCGT GGGCCTGGGC GAGCAGGAGC CCACGGTTGG CGAGCTGGAG
    CGGATGCTGG AGACTGCGGC CGTGCGTGCC CAGAAGCAGC TGGTGCTGCT GCACCGTCAG
    GACGGGCCCG CGCCGGCCCG CACCGCGGAG TGGCTCAACA TGCGGGGCTG GTGTTCTGGC
    CACCTGCACC TCTGCTGCCC TCGCCGCGTC TTCTCCAGGA GGAGCCTGCC CAAGCTGGTG
    GAGCTGTACG AGCGGGTTTG CCAGAGGCCC CCGGACCGGC ACTCGGACTT CTCCCGCCTG
    GCAAGAGTGC TGACCGGCAA TGCCATCGCC TTGGTCCTTG GGGGAGGGGG AGCAAGAGGC
    TGCGCGCAGG TGGGCATCAT CCGGGCTCTG ACGGAGTACG GCATCCCCGT GGACATGGTT
    GGAGGGACGT CCATCGGGGC CTTCATGGGC GCCCTGTACT CGGAGGAACG CAGCTACAGC
    CAGATCCGCA TCCGGGCCAA GCAGTGGGCG GAGGACATGA TGTCGATGGT GAAGACCATG
    CTGGACCTGA CCTACCCCAT CACTTCCATG TTCTCGGGGG CTGGCTTCAA CAGCAGCATC
    TCTAGCGTCT TCAAGGACAG GCAGATCGAG GACCTGTGGA TCCCATACTT CGCCATCACC
    ACGGACATCT CCGCATCTGC CATGCGGGTC CACACGGACG GATCACTGTG GAGGTACGTG
    CGTGCCAGCA TGTCCCTGTC GGGCTACATG CCCCCACTCT GCGACCCCAA AGATGGGCAC
    CTGCTGATGG ATGGGGGCTA CATCAACAAC CTCCCAGCGG ATGTGGCCAG GTCAATGGGG
    GCAAAGGTGG TTATCGCCAT TGACGTGGGC AGCCGGGATG AGACAGACCT CACCAACTAC
    GGGGATGCGC TCTCGGGATG GTGGCTCCTG TGGAAGCGCT GGAATCCCTT GGCCACGAAG
    GTCAAGGTGC TGAACATGGC TGAGATCCAG ACACGCCTGG CCTACGTGTG CTGTGTCCGG
    CAGCTGGAGA TGGTGAAGAG CAGCGACTAC TGCGAGTACC TGCGCCCTCC GATTGACAGC
    TATGGCACCC TGGAGTTCAG CAAGTTTGAT GAGATTTGTG AGGTGGGGTA CCAGCATGGC
    CGGACAGTGT TTGACATCTG GGGTCGAAGC GGTGTGCTGG AGAAGATGCT GCAGGACCGG
    CAGGGGACAA GCAAGATGAA GGCGTGCGAC GTCCTCACCT GCCCCAACGC CTCCTTCACG
    GACCTTGCTG AGATTGTATC ACGTATTGAG CCTGCCAAGG TGGCTGCAGG GGACGATGAA
    TCTGACTACC AGACTGAGTA TGAGGAGGTG CTGCTGGGAG GCCCCAAGGA CGCGTACGTT
    GACTTCCAAA GCGCCCCAGC CGACCGGGGC TCCGACTCGG AGAATGAGCC CTCGCTTCGG
    CATCAACACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221033
    Clone ID Related Accession (Same CDS sequence) XM_010810587.3
    Accession Version XM_010810587.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product patatin-like phospholipase domain-containing protein 7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810587.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC ACGAGCATCC TCTTCGTGGT CTCGGGGCTG
    CTGCATGTGT ACCAGCGGAA GATTGACAGC GAGGAGGACA CGTTCCTGTT CATGGCACGG
    CCCGGTGAGA TGGTGGGCCA GCTGGCGGTG CTCACGGGCG AGCCCCTCAT CTTCACCATC
    AGGGCCAACC GGGACTGCAG CTTCCTGTCC ATCTCCAAGG CGCACTTCTA CGACATCATG
    CGGAGGCAGC CAGCCGTGGT CCTGAGCGTG GCGCACACCG TGGTGAGGCG GGTGTCCTCC
    TTCGTGCGGC AGATCGACTT CGCCCTGGAC TGGATGGAGG TGGAGGCCGG CCGCGCCATA
    TACAGGCAGG GGGACAAGTC CGACTGCACC TACATCGTGC TCAGCGGCCG CCTGCGCGCT
    GTGATCCAGA AGGACGACGG CAAGAAGCGC CTGGCGGGCG AGTACGGCCG CGGAGACCTC
    GTGGGCGTGG TGGAGACACT CACCCACCAG GCCAGGGCAA CCACAGTGCA TGCTATTCGG
    GACTCAGAGC TGGCCAAGCT TCCCGAAGGA GCCCTGAACT CCATCAAGCG CCAGTACCCA
    CAGGTTGTGA CTCGACTGAT TCGCCTCTTG GGCGAGAAGA TCCTGGGCAG CCTCCAGCAG
    GGAGCTGCGG CAGGTCACCA GTTTGGGTTC CATACTGTGG GCAGCAAGTG GGACTCGGGG
    AACGCCGCCA GCAACCTCTC CACAGTGGCC GTCATGCCAG TGTCGGAGGA CGTGCCCCTC
    ACCGCCTTCA CCCTGGAGCT CAAGCACGCC CTCAGCGCTA TTGGCCCTGC CCTGCTGCTG
    ACCAGTGACA GCATAAAACA GCGCCTTGGC TCTGCTGCTC TGGACAGTAT CCACGAGTAC
    CGGCTGTCCA GCTGGCTGGG GCAGCAGGAG GACATCCACC GGATCGTGCT CTACCAGGCG
    GACAGCACAC TCACGCCCTG GACCCAGCGC TGCATCCGGC AGGCAGACTG CATCCTCATC
    GTGGGCCTGG GCGAGCAGGA GCCCACGGTT GGCGAGCTGG AGCGGATGCT GGAGACTGCG
    GCCGTGCGTG CCCAGAAGCA GCTGGTGCTG CTGCACCGTC AGGACGGGCC CGCGCCGGCC
    CGCACCGCGG AGTGGCTCAA CATGCGGGGC TGGTGTTCTG GCCACCTGCA CCTCTGCTGC
    CCTCGCCGCG TCTTCTCCAG GAGGAGCCTG CCCAAGCTGG TGGAGCTGTA CGAGCGGGTT
    TGCCAGAGGC CCCCGGACCG GCACTCGGAC TTCTCCCGCC TGGCAAGAGT GCTGACCGGC
    AATGCCATCG CCTTGGTCCT TGGGGGAGGG GGAGCAAGAG GCTGCGCGCA GGTGGGCATC
    ATCCGGGCTC TGACGGAGTA CGGCATCCCC GTGGACATGG TTGGAGGGAC GTCCATCGGG
    GCCTTCATGG GCGCCCTGTA CTCGGAGGAA CGCAGCTACA GCCAGATCCG CATCCGGGCC
    AAGCAGTGGG CGGAGGACAT GATGTCGATG GTGAAGACCA TGCTGGACCT GACCTACCCC
    ATCACTTCCA TGTTCTCGGG GGCTGGCTTC AACAGCAGCA TCTCTAGCGT CTTCAAGGAC
    AGGCAGATCG AGGACCTGTG GATCCCATAC TTCGCCATCA CCACGGACAT CTCCGCATCT
    GCCATGCGGG TCCACACGGA CGGATCACTG TGGAGGTACG TGCGTGCCAG CATGTCCCTG
    TCGGGCTACA TGCCCCCACT CTGCGACCCC AAAGATGGGC ACCTGCTGAT GGATGGGGGC
    TACATCAACA ACCTCCCAGC GGATGTGGCC AGGTCAATGG GGGCAAAGGT GGTTATCGCC
    ATTGACGTGG GCAGCCGGGA TGAGACAGAC CTCACCAACT ACGGGGATGC GCTCTCGGGA
    TGGTGGCTCC TGTGGAAGCG CTGGAATCCC TTGGCCACGA AGGTCAAGGT GCTGAACATG
    GCTGAGATCC AGACACGCCT GGCCTACGTG TGCTGTGTCC GGCAGCTGGA GATGGTGAAG
    AGCAGCGACT ACTGCGAGTA CCTGCGCCCT CCGATTGACA GCTATGGCAC CCTGGAGTTC
    AGCAAGTTTG ATGAGATTTG TGAGGTGGGG TACCAGCATG GCCGGACAGT GTTTGACATC
    TGGGGTCGAA GCGGTGTGCT GGAGAAGATG CTGCAGGACC GGCAGGGGAC AAGCAAGATG
    AAGGCGTGCG ACGTCCTCAC CTGCCCCAAC GCCTCCTTCA CGGACCTTGC TGAGATTGTA
    TCACGTATTG AGCCTGCCAA GGTGGCTGCA GGGGACGATG AATCTGACTA CCAGACTGAG
    TATGAGGAGG TGCTGCTGGG AGGCCCCAAG GACGCGTACG TTGACTTCCA AAGCGCCCCA
    GCCGACCGGG GCTCCGACTC GGAGAATGAG CCCTCGCTTC GGCATCAACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808889.1
    CDS188..4081
    Translation

    Target ORF information:

    RefSeq Version XM_010810587.3
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810587.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC ACGAGCATCC TCTTCGTGGT CTCGGGGCTG
    CTGCATGTGT ACCAGCGGAA GATTGACAGC GAGGAGGACA CGTTCCTGTT CATGGCACGG
    CCCGGTGAGA TGGTGGGCCA GCTGGCGGTG CTCACGGGCG AGCCCCTCAT CTTCACCATC
    AGGGCCAACC GGGACTGCAG CTTCCTGTCC ATCTCCAAGG CGCACTTCTA CGACATCATG
    CGGAGGCAGC CAGCCGTGGT CCTGAGCGTG GCGCACACCG TGGTGAGGCG GGTGTCCTCC
    TTCGTGCGGC AGATCGACTT CGCCCTGGAC TGGATGGAGG TGGAGGCCGG CCGCGCCATA
    TACAGGCAGG GGGACAAGTC CGACTGCACC TACATCGTGC TCAGCGGCCG CCTGCGCGCT
    GTGATCCAGA AGGACGACGG CAAGAAGCGC CTGGCGGGCG AGTACGGCCG CGGAGACCTC
    GTGGGCGTGG TGGAGACACT CACCCACCAG GCCAGGGCAA CCACAGTGCA TGCTATTCGG
    GACTCAGAGC TGGCCAAGCT TCCCGAAGGA GCCCTGAACT CCATCAAGCG CCAGTACCCA
    CAGGTTGTGA CTCGACTGAT TCGCCTCTTG GGCGAGAAGA TCCTGGGCAG CCTCCAGCAG
    GGAGCTGCGG CAGGTCACCA GTTTGGGTTC CATACTGTGG GCAGCAAGTG GGACTCGGGG
    AACGCCGCCA GCAACCTCTC CACAGTGGCC GTCATGCCAG TGTCGGAGGA CGTGCCCCTC
    ACCGCCTTCA CCCTGGAGCT CAAGCACGCC CTCAGCGCTA TTGGCCCTGC CCTGCTGCTG
    ACCAGTGACA GCATAAAACA GCGCCTTGGC TCTGCTGCTC TGGACAGTAT CCACGAGTAC
    CGGCTGTCCA GCTGGCTGGG GCAGCAGGAG GACATCCACC GGATCGTGCT CTACCAGGCG
    GACAGCACAC TCACGCCCTG GACCCAGCGC TGCATCCGGC AGGCAGACTG CATCCTCATC
    GTGGGCCTGG GCGAGCAGGA GCCCACGGTT GGCGAGCTGG AGCGGATGCT GGAGACTGCG
    GCCGTGCGTG CCCAGAAGCA GCTGGTGCTG CTGCACCGTC AGGACGGGCC CGCGCCGGCC
    CGCACCGCGG AGTGGCTCAA CATGCGGGGC TGGTGTTCTG GCCACCTGCA CCTCTGCTGC
    CCTCGCCGCG TCTTCTCCAG GAGGAGCCTG CCCAAGCTGG TGGAGCTGTA CGAGCGGGTT
    TGCCAGAGGC CCCCGGACCG GCACTCGGAC TTCTCCCGCC TGGCAAGAGT GCTGACCGGC
    AATGCCATCG CCTTGGTCCT TGGGGGAGGG GGAGCAAGAG GCTGCGCGCA GGTGGGCATC
    ATCCGGGCTC TGACGGAGTA CGGCATCCCC GTGGACATGG TTGGAGGGAC GTCCATCGGG
    GCCTTCATGG GCGCCCTGTA CTCGGAGGAA CGCAGCTACA GCCAGATCCG CATCCGGGCC
    AAGCAGTGGG CGGAGGACAT GATGTCGATG GTGAAGACCA TGCTGGACCT GACCTACCCC
    ATCACTTCCA TGTTCTCGGG GGCTGGCTTC AACAGCAGCA TCTCTAGCGT CTTCAAGGAC
    AGGCAGATCG AGGACCTGTG GATCCCATAC TTCGCCATCA CCACGGACAT CTCCGCATCT
    GCCATGCGGG TCCACACGGA CGGATCACTG TGGAGGTACG TGCGTGCCAG CATGTCCCTG
    TCGGGCTACA TGCCCCCACT CTGCGACCCC AAAGATGGGC ACCTGCTGAT GGATGGGGGC
    TACATCAACA ACCTCCCAGC GGATGTGGCC AGGTCAATGG GGGCAAAGGT GGTTATCGCC
    ATTGACGTGG GCAGCCGGGA TGAGACAGAC CTCACCAACT ACGGGGATGC GCTCTCGGGA
    TGGTGGCTCC TGTGGAAGCG CTGGAATCCC TTGGCCACGA AGGTCAAGGT GCTGAACATG
    GCTGAGATCC AGACACGCCT GGCCTACGTG TGCTGTGTCC GGCAGCTGGA GATGGTGAAG
    AGCAGCGACT ACTGCGAGTA CCTGCGCCCT CCGATTGACA GCTATGGCAC CCTGGAGTTC
    AGCAAGTTTG ATGAGATTTG TGAGGTGGGG TACCAGCATG GCCGGACAGT GTTTGACATC
    TGGGGTCGAA GCGGTGTGCT GGAGAAGATG CTGCAGGACC GGCAGGGGAC AAGCAAGATG
    AAGGCGTGCG ACGTCCTCAC CTGCCCCAAC GCCTCCTTCA CGGACCTTGC TGAGATTGTA
    TCACGTATTG AGCCTGCCAA GGTGGCTGCA GGGGACGATG AATCTGACTA CCAGACTGAG
    TATGAGGAGG TGCTGCTGGG AGGCCCCAAG GACGCGTACG TTGACTTCCA AAGCGCCCCA
    GCCGACCGGG GCTCCGACTC GGAGAATGAG CCCTCGCTTC GGCATCAACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221034
    Clone ID Related Accession (Same CDS sequence) XM_010810588.3
    Accession Version XM_010810588.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product patatin-like phospholipase domain-containing protein 7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810588.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGGG
    GCCACAGCAG GTGCCGCCTC AGATCTGAGG GTGGTGTGTG ACCGAGCCCG GGTCCTGCCA
    CACACAGAGG AGCACCCCGG GGGCGCTGTG GCCAGCAAGC CCAAGAAGAG TGTGGTTGTT
    GCAGAGACCC CATCGGCTGT CCTCCCCAAC TCGGACGGTA CCTTGGACGA GTCTGTCACC
    AGCAAGAAGA CAGACTCCAT CCTCAGAGCC GCCACACAGG ACCTGCTCAC CCTGATGAAG
    CTGGATGACC CTTCGCTGTT AGATGGCCGG GTGGCGCTTC TCCACGTCCC AGGGGGCACG
    GTGGTGTTGA GGCAGGGAGA CCAGGACACG AGCATCCTCT TCGTGGTCTC GGGGCTGCTG
    CATGTGTACC AGCGGAAGAT TGACAGCGAG GAGGACACGT TCCTGTTCAT GGCACGGCCC
    GGTGAGATGG TGGGCCAGCT GGCGGTGCTC ACGGGCGAGC CCCTCATCTT CACCATCAGG
    GCCAACCGGG ACTGCAGCTT CCTGTCCATC TCCAAGGCGC ACTTCTACGA CATCATGCGG
    AGGCAGCCAG CCGTGGTCCT GAGCGTGGCG CACACCGTGG TGAGGCGGGT GTCCTCCTTC
    GTGCGGCAGA TCGACTTCGC CCTGGACTGG ATGGAGGTGG AGGCCGGCCG CGCCATATAC
    AGGCAGGGGG ACAAGTCCGA CTGCACCTAC ATCGTGCTCA GCGGCCGCCT GCGCGCTGTG
    ATCCAGAAGG ACGACGGCAA GAAGCGCCTG GCGGGCGAGT ACGGCCGCGG AGACCTCGTG
    GGCGTGGTGG AGACACTCAC CCACCAGGCC AGGGCAACCA CAGTGCATGC TATTCGGGAC
    TCAGAGCTGG CCAAGCTTCC CGAAGGAGCC CTGAACTCCA TCAAGCGCCA GTACCCACAG
    GTTGTGACTC GACTGATTCG CCTCTTGGGC GAGAAGATCC TGGGCAGCCT CCAGCAGGGA
    GCTGCGGCAG GTCACCAGTT TGGGTTCCAT ACTGTGGGCA GCAAGTGGGA CTCGGGGAAC
    GCCGCCAGCA ACCTCTCCAC AGTGGCCGTC ATGCCAGTGT CGGAGGACGT GCCCCTCACC
    GCCTTCACCC TGGAGCTCAA GCACGCCCTC AGCGCTATTG GCCCTGCCCT GCTGCTGACC
    AGTGACAGCA TAAAACAGCG CCTTGGCTCT GCTGCTCTGG ACAGTATCCA CGAGTACCGG
    CTGTCCAGCT GGCTGGGGCA GCAGGAGGAC ATCCACCGGA TCGTGCTCTA CCAGGCGGAC
    AGCACACTCA CGCCCTGGAC CCAGCGCTGC ATCCGGCAGG CAGACTGCAT CCTCATCGTG
    GGCCTGGGCG AGCAGGAGCC CACGGTTGGC GAGCTGGAGC GGATGCTGGA GACTGCGGCC
    GTGCGTGCCC AGAAGCAGCT GGTGCTGCTG CACCGTCAGG ACGGGCCCGC GCCGGCCCGC
    ACCGCGGAGT GGCTCAACAT GCGGGGCTGG TGTTCTGGCC ACCTGCACCT CTGCTGCCCT
    CGCCGCGTCT TCTCCAGGAG GAGCCTGCCC AAGCTGGTGG AGCTGTACGA GCGGGTTTGC
    CAGAGGCCCC CGGACCGGCA CTCGGACTTC TCCCGCCTGG CAAGAGTGCT GACCGGCAAT
    GCCATCGCCT TGGTCCTTGG GGGAGGGGGA GCAAGAGGCT GCGCGCAGGT GGGCATCATC
    CGGGCTCTGA CGGAGTACGG CATCCCCGTG GACATGGTTG GAGGGACGTC CATCGGGGCC
    TTCATGGGCG CCCTGTACTC GGAGGAACGC AGCTACAGCC AGATCCGCAT CCGGGCCAAG
    CAGTGGGCGG AGGACATGAT GTCGATGGTG AAGACCATGC TGGACCTGAC CTACCCCATC
    ACTTCCATGT TCTCGGGGGC TGGCTTCAAC AGCAGCATCT CTAGCGTCTT CAAGGACAGG
    CAGATCGAGG ACCTGTGGAT CCCATACTTC GCCATCACCA CGGACATCTC CGCATCTGCC
    ATGCGGGTCC ACACGGACGG ATCACTGTGG AGGTACGTGC GTGCCAGCAT GTCCCTGTCG
    GGCTACATGC CCCCACTCTG CGACCCCAAA GATGGGCACC TGCTGATGGA TGGGGGCTAC
    ATCAACAACC TCCCAGCGGA TGTGGCCAGG TCAATGGGGG CAAAGGTGGT TATCGCCATT
    GACGTGGGCA GCCGGGATGA GACAGACCTC ACCAACTACG GGGATGCGCT CTCGGGATGG
    TGGCTCCTGT GGAAGCGCTG GAATCCCTTG GCCACGAAGG TCAAGGTGCT GAACATGGCT
    GAGATCCAGA CACGCCTGGC CTACGTGTGC TGTGTCCGGC AGCTGGAGAT GGTGAAGAGC
    AGCGACTACT GCGAGTACCT GCGCCCTCCG ATTGACAGCT ATGGCACCCT GGAGTTCAGC
    AAGTTTGATG AGATTTGTGA GGTGGGGTAC CAGCATGGCC GGACAGTGTT TGACATCTGG
    GGTCGAAGCG GTGTGCTGGA GAAGATGCTG CAGGACCGGC AGGGGACAAG CAAGATGAAG
    GCGTGCGACG TCCTCACCTG CCCCAACGCC TCCTTCACGG ACCTTGCTGA GATTGTATCA
    CGTATTGAGC CTGCCAAGGT GGCTGCAGGG GACGATGAAT CTGACTACCA GACTGAGTAT
    GAGGAGGTGC TGCTGGGAGG CCCCAAGGAC GCGTACGTTG ACTTCCAAAG CGCCCCAGCC
    GACCGGGGCT CCGACTCGGA GAATGAGCCC TCGCTTCGGC ATCAACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808890.1
    CDS188..4018
    Translation

    Target ORF information:

    RefSeq Version XM_010810588.3
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810588.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGGG
    GCCACAGCAG GTGCCGCCTC AGATCTGAGG GTGGTGTGTG ACCGAGCCCG GGTCCTGCCA
    CACACAGAGG AGCACCCCGG GGGCGCTGTG GCCAGCAAGC CCAAGAAGAG TGTGGTTGTT
    GCAGAGACCC CATCGGCTGT CCTCCCCAAC TCGGACGGTA CCTTGGACGA GTCTGTCACC
    AGCAAGAAGA CAGACTCCAT CCTCAGAGCC GCCACACAGG ACCTGCTCAC CCTGATGAAG
    CTGGATGACC CTTCGCTGTT AGATGGCCGG GTGGCGCTTC TCCACGTCCC AGGGGGCACG
    GTGGTGTTGA GGCAGGGAGA CCAGGACACG AGCATCCTCT TCGTGGTCTC GGGGCTGCTG
    CATGTGTACC AGCGGAAGAT TGACAGCGAG GAGGACACGT TCCTGTTCAT GGCACGGCCC
    GGTGAGATGG TGGGCCAGCT GGCGGTGCTC ACGGGCGAGC CCCTCATCTT CACCATCAGG
    GCCAACCGGG ACTGCAGCTT CCTGTCCATC TCCAAGGCGC ACTTCTACGA CATCATGCGG
    AGGCAGCCAG CCGTGGTCCT GAGCGTGGCG CACACCGTGG TGAGGCGGGT GTCCTCCTTC
    GTGCGGCAGA TCGACTTCGC CCTGGACTGG ATGGAGGTGG AGGCCGGCCG CGCCATATAC
    AGGCAGGGGG ACAAGTCCGA CTGCACCTAC ATCGTGCTCA GCGGCCGCCT GCGCGCTGTG
    ATCCAGAAGG ACGACGGCAA GAAGCGCCTG GCGGGCGAGT ACGGCCGCGG AGACCTCGTG
    GGCGTGGTGG AGACACTCAC CCACCAGGCC AGGGCAACCA CAGTGCATGC TATTCGGGAC
    TCAGAGCTGG CCAAGCTTCC CGAAGGAGCC CTGAACTCCA TCAAGCGCCA GTACCCACAG
    GTTGTGACTC GACTGATTCG CCTCTTGGGC GAGAAGATCC TGGGCAGCCT CCAGCAGGGA
    GCTGCGGCAG GTCACCAGTT TGGGTTCCAT ACTGTGGGCA GCAAGTGGGA CTCGGGGAAC
    GCCGCCAGCA ACCTCTCCAC AGTGGCCGTC ATGCCAGTGT CGGAGGACGT GCCCCTCACC
    GCCTTCACCC TGGAGCTCAA GCACGCCCTC AGCGCTATTG GCCCTGCCCT GCTGCTGACC
    AGTGACAGCA TAAAACAGCG CCTTGGCTCT GCTGCTCTGG ACAGTATCCA CGAGTACCGG
    CTGTCCAGCT GGCTGGGGCA GCAGGAGGAC ATCCACCGGA TCGTGCTCTA CCAGGCGGAC
    AGCACACTCA CGCCCTGGAC CCAGCGCTGC ATCCGGCAGG CAGACTGCAT CCTCATCGTG
    GGCCTGGGCG AGCAGGAGCC CACGGTTGGC GAGCTGGAGC GGATGCTGGA GACTGCGGCC
    GTGCGTGCCC AGAAGCAGCT GGTGCTGCTG CACCGTCAGG ACGGGCCCGC GCCGGCCCGC
    ACCGCGGAGT GGCTCAACAT GCGGGGCTGG TGTTCTGGCC ACCTGCACCT CTGCTGCCCT
    CGCCGCGTCT TCTCCAGGAG GAGCCTGCCC AAGCTGGTGG AGCTGTACGA GCGGGTTTGC
    CAGAGGCCCC CGGACCGGCA CTCGGACTTC TCCCGCCTGG CAAGAGTGCT GACCGGCAAT
    GCCATCGCCT TGGTCCTTGG GGGAGGGGGA GCAAGAGGCT GCGCGCAGGT GGGCATCATC
    CGGGCTCTGA CGGAGTACGG CATCCCCGTG GACATGGTTG GAGGGACGTC CATCGGGGCC
    TTCATGGGCG CCCTGTACTC GGAGGAACGC AGCTACAGCC AGATCCGCAT CCGGGCCAAG
    CAGTGGGCGG AGGACATGAT GTCGATGGTG AAGACCATGC TGGACCTGAC CTACCCCATC
    ACTTCCATGT TCTCGGGGGC TGGCTTCAAC AGCAGCATCT CTAGCGTCTT CAAGGACAGG
    CAGATCGAGG ACCTGTGGAT CCCATACTTC GCCATCACCA CGGACATCTC CGCATCTGCC
    ATGCGGGTCC ACACGGACGG ATCACTGTGG AGGTACGTGC GTGCCAGCAT GTCCCTGTCG
    GGCTACATGC CCCCACTCTG CGACCCCAAA GATGGGCACC TGCTGATGGA TGGGGGCTAC
    ATCAACAACC TCCCAGCGGA TGTGGCCAGG TCAATGGGGG CAAAGGTGGT TATCGCCATT
    GACGTGGGCA GCCGGGATGA GACAGACCTC ACCAACTACG GGGATGCGCT CTCGGGATGG
    TGGCTCCTGT GGAAGCGCTG GAATCCCTTG GCCACGAAGG TCAAGGTGCT GAACATGGCT
    GAGATCCAGA CACGCCTGGC CTACGTGTGC TGTGTCCGGC AGCTGGAGAT GGTGAAGAGC
    AGCGACTACT GCGAGTACCT GCGCCCTCCG ATTGACAGCT ATGGCACCCT GGAGTTCAGC
    AAGTTTGATG AGATTTGTGA GGTGGGGTAC CAGCATGGCC GGACAGTGTT TGACATCTGG
    GGTCGAAGCG GTGTGCTGGA GAAGATGCTG CAGGACCGGC AGGGGACAAG CAAGATGAAG
    GCGTGCGACG TCCTCACCTG CCCCAACGCC TCCTTCACGG ACCTTGCTGA GATTGTATCA
    CGTATTGAGC CTGCCAAGGT GGCTGCAGGG GACGATGAAT CTGACTACCA GACTGAGTAT
    GAGGAGGTGC TGCTGGGAGG CCCCAAGGAC GCGTACGTTG ACTTCCAAAG CGCCCCAGCC
    GACCGGGGCT CCGACTCGGA GAATGAGCCC TCGCTTCGGC ATCAACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53358
    Clone ID Related Accession (Same CDS sequence) XM_010810580.2 , XM_015465574.1
    Accession Version XM_010810580.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product patatin-like phospholipase domain-containing protein 7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104304.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810580.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC CCTTCGCTGT TAGATGGCCG GGTGGCGCTT
    CTCCACGTCC CAGGGGGCAC GGTGGTGTTG AGGCAGGGAG ACCAGGACAC GAGCATCCTC
    TTCGTGGTCT CGGGGCTGCT GCATGTGTAC CAGCGGAAGA TTGACAGCGA GGAGGACACG
    TTCCTGTTCA TGGCACGGCC CGGTGAGATG GTGGGCCAGC TGGCGGTGCT CACGGGCGAG
    CCCCTCATCT TCACCATCAG GGCCAACCGG GACTGCAGCT TCCTGTCCAT CTCCAAGGCG
    CACTTCTACG ACATCATGCG GAGGCAGCCA GCCGTGGTCC TGAGCGTGGC GCACACCGTG
    GTGAGGCGGG TGTCCTCCTT CGTGCGGCAG ATCGACTTCG CCCTGGACTG GATGGAGGTG
    GAGGCCGGCC GCGCCATATA CAGGCAGGGG GACAAGTCCG ACTGCACCTA CATCGTGCTC
    AGCGGCCGCC TGCGCGCTGT GATCCAGAAG GACGACGGCA AGAAGCGCCT GGCGGGCGAG
    TACGGCCGCG GAGACCTCGT GGGCGTGGTG GAGACACTCA CCCACCAGGC CAGGGCAACC
    ACAGTGCATG CTATTCGGGA CTCAGAGCTG GCCAAGCTTC CCGAAGGAGC CCTGAACTCC
    ATCAAGCGCC AGTACCCACA GGTTGTGACT CGACTGATTC GCCTCTTGGG CGAGAAGATC
    CTGGGCAGCC TCCAGCAGGG AGCTGCGGCA GGTCACCAGT TTGGGTTCCA TACTGTGGGC
    AGCAAGTGGG ACTCGGGGAA CGCCGCCAGC AACCTCTCCA CAGTGGCCGT CATGCCAGTG
    TCGGAGGACG TGCCCCTCAC CGCCTTCACC CTGGAGCTCA AGCACGCCCT CAGCGCTATT
    GGCCCTGCCC TGCTGCTGAC CAGTGACAGC ATAAAACAGC GCCTTGGCTC TGCTGCTCTG
    GACAGTATCC ACGAGTACCG GCTGTCCAGC TGGCTGGGGC AGCAGGAGGA CATCCACCGG
    ATCGTGCTCT ACCAGGCGGA CAGCACACTC ACGCCCTGGA CCCAGCGCTG CATCCGGCAG
    GCAGACTGCA TCCTCATCGT GGGCCTGGGC GAGCAGGAGC CCACGGTTGG CGAGCTGGAG
    CGGATGCTGG AGACTGCGGC CGTGCGTGCC CAGAAGCAGC TGGTGCTGCT GCACCGTCAG
    GACGGGCCCG CGCCGGCCCG CACCGCGGAG TGGCTCAACA TGCGGGGCTG GTGTTCTGGC
    CACCTGCACC TCTGCTGCCC TCGCCGCGTC TTCTCCAGGA GGAGCCTGCC CAAGCTGGTG
    GAGCTGTACG AGCGGGTTTG CCAGAGGCCC CCGGACCGGC ACTCGGACTT CTCCCGCCTG
    GCAAGAGTGC TGACCGGCAA TGCCATCGCC TTGGTCCTTG GGGGAGGGGG AGCAAGAGGC
    TGCGCGCAGG TGGGCATCAT CCGGGCTCTG ACGGAGTACG GCATCCCCGT GGACATGGTT
    GGAGGGACGT CCATCGGGGC CTTCATGGGC GCCCTGTACT CGGAGGAACG CAGCTACAGC
    CAGATCCGCA TCCGGGCCAA GCAGTGGGCG GAGGACATGA TGTCGATGGT GAAGACCATG
    CTGGACCTGA CCTACCCCAT CACTTCCATG TTCTCGGGGG CTGGCTTCAA CAGCAGCATC
    TCTAGCGTCT TCAAGGACAG GCAGATCGAG GACCTGTGGA TCCCATACTT CGCCATCACC
    ACGGACATCT CCGCATCTGC CATGCGGGTC CACACGGACG GATCACTGTG GAGGTACGTG
    CGTGCCAGCA TGTCCCTGTC GGGCTACATG CCCCCACTCT GCGACCCCAA AGATGGGCAC
    CTGCTGATGG ATGGGGGCTA CATCAACAAC CTCCCAGCGG ATGTGGCCAG GTCAATGGGG
    GCAAAGGTGG TGATCGCCAT TGACGTGGGC AGCCGGGATG AGACAGACCT CACCAACTAC
    GGGGATGCGC TCTCGGGATG GTGGCTCCTG TGGAAGCGCT GGAATCCCTT GGCCACGAAG
    GTCAAGGTGC TGAACATGGC TGAGATCCAG ACACGCCTGG CCTACGTGTG CTGTGTCCGG
    CAGCTGGAGA TGGTGAAGAG CAGCGACTAC TGCGAGTACC TGCGCCCTCC GATTGACAGC
    TATGGCACCC TGGAGTTCAG CAAGTTTGAT GAGATTTGTG AGGTGGGGTA CCAGCATGGC
    CGGACAGTGT TTGACATCTG GGGTCGAAGC GGTGTGCTGG AGAAGATGCT GCAGGACCGG
    CAGGGGACAA GCAAGATGAA GGCGTGCGAC GTCCTCACCT GCCCCAACGC CTCCTTCACG
    GACCTTGCTG AGATTGTATC ACGTATTGAG CCTGCCAAGG TGGCTGCAGG GGACGATGAA
    TCTGACTACC AGACTGAGTA TGAGGAGGTG CTGCTGGGAG GCCCCAAGGA CGCGTACGTT
    GACTTCCAAA GCGCCCCAGC CGACCGGGGC TCCGACTCGG AGAATGAGCC CTCGCTTCGG
    CATCAACACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808882.1
    CDS176..4147
    Translation

    Target ORF information:

    RefSeq Version XM_010810580.2
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810580.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC CCTTCGCTGT TAGATGGCCG GGTGGCGCTT
    CTCCACGTCC CAGGGGGCAC GGTGGTGTTG AGGCAGGGAG ACCAGGACAC GAGCATCCTC
    TTCGTGGTCT CGGGGCTGCT GCATGTGTAC CAGCGGAAGA TTGACAGCGA GGAGGACACG
    TTCCTGTTCA TGGCACGGCC CGGTGAGATG GTGGGCCAGC TGGCGGTGCT CACGGGCGAG
    CCCCTCATCT TCACCATCAG GGCCAACCGG GACTGCAGCT TCCTGTCCAT CTCCAAGGCG
    CACTTCTACG ACATCATGCG GAGGCAGCCA GCCGTGGTCC TGAGCGTGGC GCACACCGTG
    GTGAGGCGGG TGTCCTCCTT CGTGCGGCAG ATCGACTTCG CCCTGGACTG GATGGAGGTG
    GAGGCCGGCC GCGCCATATA CAGGCAGGGG GACAAGTCCG ACTGCACCTA CATCGTGCTC
    AGCGGCCGCC TGCGCGCTGT GATCCAGAAG GACGACGGCA AGAAGCGCCT GGCGGGCGAG
    TACGGCCGCG GAGACCTCGT GGGCGTGGTG GAGACACTCA CCCACCAGGC CAGGGCAACC
    ACAGTGCATG CTATTCGGGA CTCAGAGCTG GCCAAGCTTC CCGAAGGAGC CCTGAACTCC
    ATCAAGCGCC AGTACCCACA GGTTGTGACT CGACTGATTC GCCTCTTGGG CGAGAAGATC
    CTGGGCAGCC TCCAGCAGGG AGCTGCGGCA GGTCACCAGT TTGGGTTCCA TACTGTGGGC
    AGCAAGTGGG ACTCGGGGAA CGCCGCCAGC AACCTCTCCA CAGTGGCCGT CATGCCAGTG
    TCGGAGGACG TGCCCCTCAC CGCCTTCACC CTGGAGCTCA AGCACGCCCT CAGCGCTATT
    GGCCCTGCCC TGCTGCTGAC CAGTGACAGC ATAAAACAGC GCCTTGGCTC TGCTGCTCTG
    GACAGTATCC ACGAGTACCG GCTGTCCAGC TGGCTGGGGC AGCAGGAGGA CATCCACCGG
    ATCGTGCTCT ACCAGGCGGA CAGCACACTC ACGCCCTGGA CCCAGCGCTG CATCCGGCAG
    GCAGACTGCA TCCTCATCGT GGGCCTGGGC GAGCAGGAGC CCACGGTTGG CGAGCTGGAG
    CGGATGCTGG AGACTGCGGC CGTGCGTGCC CAGAAGCAGC TGGTGCTGCT GCACCGTCAG
    GACGGGCCCG CGCCGGCCCG CACCGCGGAG TGGCTCAACA TGCGGGGCTG GTGTTCTGGC
    CACCTGCACC TCTGCTGCCC TCGCCGCGTC TTCTCCAGGA GGAGCCTGCC CAAGCTGGTG
    GAGCTGTACG AGCGGGTTTG CCAGAGGCCC CCGGACCGGC ACTCGGACTT CTCCCGCCTG
    GCAAGAGTGC TGACCGGCAA TGCCATCGCC TTGGTCCTTG GGGGAGGGGG AGCAAGAGGC
    TGCGCGCAGG TGGGCATCAT CCGGGCTCTG ACGGAGTACG GCATCCCCGT GGACATGGTT
    GGAGGGACGT CCATCGGGGC CTTCATGGGC GCCCTGTACT CGGAGGAACG CAGCTACAGC
    CAGATCCGCA TCCGGGCCAA GCAGTGGGCG GAGGACATGA TGTCGATGGT GAAGACCATG
    CTGGACCTGA CCTACCCCAT CACTTCCATG TTCTCGGGGG CTGGCTTCAA CAGCAGCATC
    TCTAGCGTCT TCAAGGACAG GCAGATCGAG GACCTGTGGA TCCCATACTT CGCCATCACC
    ACGGACATCT CCGCATCTGC CATGCGGGTC CACACGGACG GATCACTGTG GAGGTACGTG
    CGTGCCAGCA TGTCCCTGTC GGGCTACATG CCCCCACTCT GCGACCCCAA AGATGGGCAC
    CTGCTGATGG ATGGGGGCTA CATCAACAAC CTCCCAGCGG ATGTGGCCAG GTCAATGGGG
    GCAAAGGTGG TGATCGCCAT TGACGTGGGC AGCCGGGATG AGACAGACCT CACCAACTAC
    GGGGATGCGC TCTCGGGATG GTGGCTCCTG TGGAAGCGCT GGAATCCCTT GGCCACGAAG
    GTCAAGGTGC TGAACATGGC TGAGATCCAG ACACGCCTGG CCTACGTGTG CTGTGTCCGG
    CAGCTGGAGA TGGTGAAGAG CAGCGACTAC TGCGAGTACC TGCGCCCTCC GATTGACAGC
    TATGGCACCC TGGAGTTCAG CAAGTTTGAT GAGATTTGTG AGGTGGGGTA CCAGCATGGC
    CGGACAGTGT TTGACATCTG GGGTCGAAGC GGTGTGCTGG AGAAGATGCT GCAGGACCGG
    CAGGGGACAA GCAAGATGAA GGCGTGCGAC GTCCTCACCT GCCCCAACGC CTCCTTCACG
    GACCTTGCTG AGATTGTATC ACGTATTGAG CCTGCCAAGG TGGCTGCAGG GGACGATGAA
    TCTGACTACC AGACTGAGTA TGAGGAGGTG CTGCTGGGAG GCCCCAAGGA CGCGTACGTT
    GACTTCCAAA GCGCCCCAGC CGACCGGGGC TCCGACTCGG AGAATGAGCC CTCGCTTCGG
    CATCAACACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53358
    Clone ID Related Accession (Same CDS sequence) XM_010810580.2 , XM_015465574.1
    Accession Version XM_015465574.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product patatin-like phospholipase domain-containing protein 7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC CCTTCGCTGT TAGATGGCCG GGTGGCGCTT
    CTCCACGTCC CAGGGGGCAC GGTGGTGTTG AGGCAGGGAG ACCAGGACAC GAGCATCCTC
    TTCGTGGTCT CGGGGCTGCT GCATGTGTAC CAGCGGAAGA TTGACAGCGA GGAGGACACG
    TTCCTGTTCA TGGCACGGCC CGGTGAGATG GTGGGCCAGC TGGCGGTGCT CACGGGCGAG
    CCCCTCATCT TCACCATCAG GGCCAACCGG GACTGCAGCT TCCTGTCCAT CTCCAAGGCG
    CACTTCTACG ACATCATGCG GAGGCAGCCA GCCGTGGTCC TGAGCGTGGC GCACACCGTG
    GTGAGGCGGG TGTCCTCCTT CGTGCGGCAG ATCGACTTCG CCCTGGACTG GATGGAGGTG
    GAGGCCGGCC GCGCCATATA CAGGCAGGGG GACAAGTCCG ACTGCACCTA CATCGTGCTC
    AGCGGCCGCC TGCGCGCTGT GATCCAGAAG GACGACGGCA AGAAGCGCCT GGCGGGCGAG
    TACGGCCGCG GAGACCTCGT GGGCGTGGTG GAGACACTCA CCCACCAGGC CAGGGCAACC
    ACAGTGCATG CTATTCGGGA CTCAGAGCTG GCCAAGCTTC CCGAAGGAGC CCTGAACTCC
    ATCAAGCGCC AGTACCCACA GGTTGTGACT CGACTGATTC GCCTCTTGGG CGAGAAGATC
    CTGGGCAGCC TCCAGCAGGG AGCTGCGGCA GGTCACCAGT TTGGGTTCCA TACTGTGGGC
    AGCAAGTGGG ACTCGGGGAA CGCCGCCAGC AACCTCTCCA CAGTGGCCGT CATGCCAGTG
    TCGGAGGACG TGCCCCTCAC CGCCTTCACC CTGGAGCTCA AGCACGCCCT CAGCGCTATT
    GGCCCTGCCC TGCTGCTGAC CAGTGACAGC ATAAAACAGC GCCTTGGCTC TGCTGCTCTG
    GACAGTATCC ACGAGTACCG GCTGTCCAGC TGGCTGGGGC AGCAGGAGGA CATCCACCGG
    ATCGTGCTCT ACCAGGCGGA CAGCACACTC ACGCCCTGGA CCCAGCGCTG CATCCGGCAG
    GCAGACTGCA TCCTCATCGT GGGCCTGGGC GAGCAGGAGC CCACGGTTGG CGAGCTGGAG
    CGGATGCTGG AGACTGCGGC CGTGCGTGCC CAGAAGCAGC TGGTGCTGCT GCACCGTCAG
    GACGGGCCCG CGCCGGCCCG CACCGCGGAG TGGCTCAACA TGCGGGGCTG GTGTTCTGGC
    CACCTGCACC TCTGCTGCCC TCGCCGCGTC TTCTCCAGGA GGAGCCTGCC CAAGCTGGTG
    GAGCTGTACG AGCGGGTTTG CCAGAGGCCC CCGGACCGGC ACTCGGACTT CTCCCGCCTG
    GCAAGAGTGC TGACCGGCAA TGCCATCGCC TTGGTCCTTG GGGGAGGGGG AGCAAGAGGC
    TGCGCGCAGG TGGGCATCAT CCGGGCTCTG ACGGAGTACG GCATCCCCGT GGACATGGTT
    GGAGGGACGT CCATCGGGGC CTTCATGGGC GCCCTGTACT CGGAGGAACG CAGCTACAGC
    CAGATCCGCA TCCGGGCCAA GCAGTGGGCG GAGGACATGA TGTCGATGGT GAAGACCATG
    CTGGACCTGA CCTACCCCAT CACTTCCATG TTCTCGGGGG CTGGCTTCAA CAGCAGCATC
    TCTAGCGTCT TCAAGGACAG GCAGATCGAG GACCTGTGGA TCCCATACTT CGCCATCACC
    ACGGACATCT CCGCATCTGC CATGCGGGTC CACACGGACG GATCACTGTG GAGGTACGTG
    CGTGCCAGCA TGTCCCTGTC GGGCTACATG CCCCCACTCT GCGACCCCAA AGATGGGCAC
    CTGCTGATGG ATGGGGGCTA CATCAACAAC CTCCCAGCGG ATGTGGCCAG GTCAATGGGG
    GCAAAGGTGG TGATCGCCAT TGACGTGGGC AGCCGGGATG AGACAGACCT CACCAACTAC
    GGGGATGCGC TCTCGGGATG GTGGCTCCTG TGGAAGCGCT GGAATCCCTT GGCCACGAAG
    GTCAAGGTGC TGAACATGGC TGAGATCCAG ACACGCCTGG CCTACGTGTG CTGTGTCCGG
    CAGCTGGAGA TGGTGAAGAG CAGCGACTAC TGCGAGTACC TGCGCCCTCC GATTGACAGC
    TATGGCACCC TGGAGTTCAG CAAGTTTGAT GAGATTTGTG AGGTGGGGTA CCAGCATGGC
    CGGACAGTGT TTGACATCTG GGGTCGAAGC GGTGTGCTGG AGAAGATGCT GCAGGACCGG
    CAGGGGACAA GCAAGATGAA GGCGTGCGAC GTCCTCACCT GCCCCAACGC CTCCTTCACG
    GACCTTGCTG AGATTGTATC ACGTATTGAG CCTGCCAAGG TGGCTGCAGG GGACGATGAA
    TCTGACTACC AGACTGAGTA TGAGGAGGTG CTGCTGGGAG GCCCCAAGGA CGCGTACGTT
    GACTTCCAAA GCGCCCCAGC CGACCGGGGC TCCGACTCGG AGAATGAGCC CTCGCTTCGG
    CATCAACACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321060.1
    CDS176..4147
    Translation

    Target ORF information:

    RefSeq Version XM_015465574.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465574.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC CCTTCGCTGT TAGATGGCCG GGTGGCGCTT
    CTCCACGTCC CAGGGGGCAC GGTGGTGTTG AGGCAGGGAG ACCAGGACAC GAGCATCCTC
    TTCGTGGTCT CGGGGCTGCT GCATGTGTAC CAGCGGAAGA TTGACAGCGA GGAGGACACG
    TTCCTGTTCA TGGCACGGCC CGGTGAGATG GTGGGCCAGC TGGCGGTGCT CACGGGCGAG
    CCCCTCATCT TCACCATCAG GGCCAACCGG GACTGCAGCT TCCTGTCCAT CTCCAAGGCG
    CACTTCTACG ACATCATGCG GAGGCAGCCA GCCGTGGTCC TGAGCGTGGC GCACACCGTG
    GTGAGGCGGG TGTCCTCCTT CGTGCGGCAG ATCGACTTCG CCCTGGACTG GATGGAGGTG
    GAGGCCGGCC GCGCCATATA CAGGCAGGGG GACAAGTCCG ACTGCACCTA CATCGTGCTC
    AGCGGCCGCC TGCGCGCTGT GATCCAGAAG GACGACGGCA AGAAGCGCCT GGCGGGCGAG
    TACGGCCGCG GAGACCTCGT GGGCGTGGTG GAGACACTCA CCCACCAGGC CAGGGCAACC
    ACAGTGCATG CTATTCGGGA CTCAGAGCTG GCCAAGCTTC CCGAAGGAGC CCTGAACTCC
    ATCAAGCGCC AGTACCCACA GGTTGTGACT CGACTGATTC GCCTCTTGGG CGAGAAGATC
    CTGGGCAGCC TCCAGCAGGG AGCTGCGGCA GGTCACCAGT TTGGGTTCCA TACTGTGGGC
    AGCAAGTGGG ACTCGGGGAA CGCCGCCAGC AACCTCTCCA CAGTGGCCGT CATGCCAGTG
    TCGGAGGACG TGCCCCTCAC CGCCTTCACC CTGGAGCTCA AGCACGCCCT CAGCGCTATT
    GGCCCTGCCC TGCTGCTGAC CAGTGACAGC ATAAAACAGC GCCTTGGCTC TGCTGCTCTG
    GACAGTATCC ACGAGTACCG GCTGTCCAGC TGGCTGGGGC AGCAGGAGGA CATCCACCGG
    ATCGTGCTCT ACCAGGCGGA CAGCACACTC ACGCCCTGGA CCCAGCGCTG CATCCGGCAG
    GCAGACTGCA TCCTCATCGT GGGCCTGGGC GAGCAGGAGC CCACGGTTGG CGAGCTGGAG
    CGGATGCTGG AGACTGCGGC CGTGCGTGCC CAGAAGCAGC TGGTGCTGCT GCACCGTCAG
    GACGGGCCCG CGCCGGCCCG CACCGCGGAG TGGCTCAACA TGCGGGGCTG GTGTTCTGGC
    CACCTGCACC TCTGCTGCCC TCGCCGCGTC TTCTCCAGGA GGAGCCTGCC CAAGCTGGTG
    GAGCTGTACG AGCGGGTTTG CCAGAGGCCC CCGGACCGGC ACTCGGACTT CTCCCGCCTG
    GCAAGAGTGC TGACCGGCAA TGCCATCGCC TTGGTCCTTG GGGGAGGGGG AGCAAGAGGC
    TGCGCGCAGG TGGGCATCAT CCGGGCTCTG ACGGAGTACG GCATCCCCGT GGACATGGTT
    GGAGGGACGT CCATCGGGGC CTTCATGGGC GCCCTGTACT CGGAGGAACG CAGCTACAGC
    CAGATCCGCA TCCGGGCCAA GCAGTGGGCG GAGGACATGA TGTCGATGGT GAAGACCATG
    CTGGACCTGA CCTACCCCAT CACTTCCATG TTCTCGGGGG CTGGCTTCAA CAGCAGCATC
    TCTAGCGTCT TCAAGGACAG GCAGATCGAG GACCTGTGGA TCCCATACTT CGCCATCACC
    ACGGACATCT CCGCATCTGC CATGCGGGTC CACACGGACG GATCACTGTG GAGGTACGTG
    CGTGCCAGCA TGTCCCTGTC GGGCTACATG CCCCCACTCT GCGACCCCAA AGATGGGCAC
    CTGCTGATGG ATGGGGGCTA CATCAACAAC CTCCCAGCGG ATGTGGCCAG GTCAATGGGG
    GCAAAGGTGG TGATCGCCAT TGACGTGGGC AGCCGGGATG AGACAGACCT CACCAACTAC
    GGGGATGCGC TCTCGGGATG GTGGCTCCTG TGGAAGCGCT GGAATCCCTT GGCCACGAAG
    GTCAAGGTGC TGAACATGGC TGAGATCCAG ACACGCCTGG CCTACGTGTG CTGTGTCCGG
    CAGCTGGAGA TGGTGAAGAG CAGCGACTAC TGCGAGTACC TGCGCCCTCC GATTGACAGC
    TATGGCACCC TGGAGTTCAG CAAGTTTGAT GAGATTTGTG AGGTGGGGTA CCAGCATGGC
    CGGACAGTGT TTGACATCTG GGGTCGAAGC GGTGTGCTGG AGAAGATGCT GCAGGACCGG
    CAGGGGACAA GCAAGATGAA GGCGTGCGAC GTCCTCACCT GCCCCAACGC CTCCTTCACG
    GACCTTGCTG AGATTGTATC ACGTATTGAG CCTGCCAAGG TGGCTGCAGG GGACGATGAA
    TCTGACTACC AGACTGAGTA TGAGGAGGTG CTGCTGGGAG GCCCCAAGGA CGCGTACGTT
    GACTTCCAAA GCGCCCCAGC CGACCGGGGC TCCGACTCGG AGAATGAGCC CTCGCTTCGG
    CATCAACACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53359
    Clone ID Related Accession (Same CDS sequence) XM_010810587.2 , XM_015465575.1
    Accession Version XM_010810587.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product patatin-like phospholipase domain-containing protein 7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104304.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810587.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC ACGAGCATCC TCTTCGTGGT CTCGGGGCTG
    CTGCATGTGT ACCAGCGGAA GATTGACAGC GAGGAGGACA CGTTCCTGTT CATGGCACGG
    CCCGGTGAGA TGGTGGGCCA GCTGGCGGTG CTCACGGGCG AGCCCCTCAT CTTCACCATC
    AGGGCCAACC GGGACTGCAG CTTCCTGTCC ATCTCCAAGG CGCACTTCTA CGACATCATG
    CGGAGGCAGC CAGCCGTGGT CCTGAGCGTG GCGCACACCG TGGTGAGGCG GGTGTCCTCC
    TTCGTGCGGC AGATCGACTT CGCCCTGGAC TGGATGGAGG TGGAGGCCGG CCGCGCCATA
    TACAGGCAGG GGGACAAGTC CGACTGCACC TACATCGTGC TCAGCGGCCG CCTGCGCGCT
    GTGATCCAGA AGGACGACGG CAAGAAGCGC CTGGCGGGCG AGTACGGCCG CGGAGACCTC
    GTGGGCGTGG TGGAGACACT CACCCACCAG GCCAGGGCAA CCACAGTGCA TGCTATTCGG
    GACTCAGAGC TGGCCAAGCT TCCCGAAGGA GCCCTGAACT CCATCAAGCG CCAGTACCCA
    CAGGTTGTGA CTCGACTGAT TCGCCTCTTG GGCGAGAAGA TCCTGGGCAG CCTCCAGCAG
    GGAGCTGCGG CAGGTCACCA GTTTGGGTTC CATACTGTGG GCAGCAAGTG GGACTCGGGG
    AACGCCGCCA GCAACCTCTC CACAGTGGCC GTCATGCCAG TGTCGGAGGA CGTGCCCCTC
    ACCGCCTTCA CCCTGGAGCT CAAGCACGCC CTCAGCGCTA TTGGCCCTGC CCTGCTGCTG
    ACCAGTGACA GCATAAAACA GCGCCTTGGC TCTGCTGCTC TGGACAGTAT CCACGAGTAC
    CGGCTGTCCA GCTGGCTGGG GCAGCAGGAG GACATCCACC GGATCGTGCT CTACCAGGCG
    GACAGCACAC TCACGCCCTG GACCCAGCGC TGCATCCGGC AGGCAGACTG CATCCTCATC
    GTGGGCCTGG GCGAGCAGGA GCCCACGGTT GGCGAGCTGG AGCGGATGCT GGAGACTGCG
    GCCGTGCGTG CCCAGAAGCA GCTGGTGCTG CTGCACCGTC AGGACGGGCC CGCGCCGGCC
    CGCACCGCGG AGTGGCTCAA CATGCGGGGC TGGTGTTCTG GCCACCTGCA CCTCTGCTGC
    CCTCGCCGCG TCTTCTCCAG GAGGAGCCTG CCCAAGCTGG TGGAGCTGTA CGAGCGGGTT
    TGCCAGAGGC CCCCGGACCG GCACTCGGAC TTCTCCCGCC TGGCAAGAGT GCTGACCGGC
    AATGCCATCG CCTTGGTCCT TGGGGGAGGG GGAGCAAGAG GCTGCGCGCA GGTGGGCATC
    ATCCGGGCTC TGACGGAGTA CGGCATCCCC GTGGACATGG TTGGAGGGAC GTCCATCGGG
    GCCTTCATGG GCGCCCTGTA CTCGGAGGAA CGCAGCTACA GCCAGATCCG CATCCGGGCC
    AAGCAGTGGG CGGAGGACAT GATGTCGATG GTGAAGACCA TGCTGGACCT GACCTACCCC
    ATCACTTCCA TGTTCTCGGG GGCTGGCTTC AACAGCAGCA TCTCTAGCGT CTTCAAGGAC
    AGGCAGATCG AGGACCTGTG GATCCCATAC TTCGCCATCA CCACGGACAT CTCCGCATCT
    GCCATGCGGG TCCACACGGA CGGATCACTG TGGAGGTACG TGCGTGCCAG CATGTCCCTG
    TCGGGCTACA TGCCCCCACT CTGCGACCCC AAAGATGGGC ACCTGCTGAT GGATGGGGGC
    TACATCAACA ACCTCCCAGC GGATGTGGCC AGGTCAATGG GGGCAAAGGT GGTGATCGCC
    ATTGACGTGG GCAGCCGGGA TGAGACAGAC CTCACCAACT ACGGGGATGC GCTCTCGGGA
    TGGTGGCTCC TGTGGAAGCG CTGGAATCCC TTGGCCACGA AGGTCAAGGT GCTGAACATG
    GCTGAGATCC AGACACGCCT GGCCTACGTG TGCTGTGTCC GGCAGCTGGA GATGGTGAAG
    AGCAGCGACT ACTGCGAGTA CCTGCGCCCT CCGATTGACA GCTATGGCAC CCTGGAGTTC
    AGCAAGTTTG ATGAGATTTG TGAGGTGGGG TACCAGCATG GCCGGACAGT GTTTGACATC
    TGGGGTCGAA GCGGTGTGCT GGAGAAGATG CTGCAGGACC GGCAGGGGAC AAGCAAGATG
    AAGGCGTGCG ACGTCCTCAC CTGCCCCAAC GCCTCCTTCA CGGACCTTGC TGAGATTGTA
    TCACGTATTG AGCCTGCCAA GGTGGCTGCA GGGGACGATG AATCTGACTA CCAGACTGAG
    TATGAGGAGG TGCTGCTGGG AGGCCCCAAG GACGCGTACG TTGACTTCCA AAGCGCCCCA
    GCCGACCGGG GCTCCGACTC GGAGAATGAG CCCTCGCTTC GGCATCAACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808889.1
    CDS176..4069
    Translation

    Target ORF information:

    RefSeq Version XM_010810587.2
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810587.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC ACGAGCATCC TCTTCGTGGT CTCGGGGCTG
    CTGCATGTGT ACCAGCGGAA GATTGACAGC GAGGAGGACA CGTTCCTGTT CATGGCACGG
    CCCGGTGAGA TGGTGGGCCA GCTGGCGGTG CTCACGGGCG AGCCCCTCAT CTTCACCATC
    AGGGCCAACC GGGACTGCAG CTTCCTGTCC ATCTCCAAGG CGCACTTCTA CGACATCATG
    CGGAGGCAGC CAGCCGTGGT CCTGAGCGTG GCGCACACCG TGGTGAGGCG GGTGTCCTCC
    TTCGTGCGGC AGATCGACTT CGCCCTGGAC TGGATGGAGG TGGAGGCCGG CCGCGCCATA
    TACAGGCAGG GGGACAAGTC CGACTGCACC TACATCGTGC TCAGCGGCCG CCTGCGCGCT
    GTGATCCAGA AGGACGACGG CAAGAAGCGC CTGGCGGGCG AGTACGGCCG CGGAGACCTC
    GTGGGCGTGG TGGAGACACT CACCCACCAG GCCAGGGCAA CCACAGTGCA TGCTATTCGG
    GACTCAGAGC TGGCCAAGCT TCCCGAAGGA GCCCTGAACT CCATCAAGCG CCAGTACCCA
    CAGGTTGTGA CTCGACTGAT TCGCCTCTTG GGCGAGAAGA TCCTGGGCAG CCTCCAGCAG
    GGAGCTGCGG CAGGTCACCA GTTTGGGTTC CATACTGTGG GCAGCAAGTG GGACTCGGGG
    AACGCCGCCA GCAACCTCTC CACAGTGGCC GTCATGCCAG TGTCGGAGGA CGTGCCCCTC
    ACCGCCTTCA CCCTGGAGCT CAAGCACGCC CTCAGCGCTA TTGGCCCTGC CCTGCTGCTG
    ACCAGTGACA GCATAAAACA GCGCCTTGGC TCTGCTGCTC TGGACAGTAT CCACGAGTAC
    CGGCTGTCCA GCTGGCTGGG GCAGCAGGAG GACATCCACC GGATCGTGCT CTACCAGGCG
    GACAGCACAC TCACGCCCTG GACCCAGCGC TGCATCCGGC AGGCAGACTG CATCCTCATC
    GTGGGCCTGG GCGAGCAGGA GCCCACGGTT GGCGAGCTGG AGCGGATGCT GGAGACTGCG
    GCCGTGCGTG CCCAGAAGCA GCTGGTGCTG CTGCACCGTC AGGACGGGCC CGCGCCGGCC
    CGCACCGCGG AGTGGCTCAA CATGCGGGGC TGGTGTTCTG GCCACCTGCA CCTCTGCTGC
    CCTCGCCGCG TCTTCTCCAG GAGGAGCCTG CCCAAGCTGG TGGAGCTGTA CGAGCGGGTT
    TGCCAGAGGC CCCCGGACCG GCACTCGGAC TTCTCCCGCC TGGCAAGAGT GCTGACCGGC
    AATGCCATCG CCTTGGTCCT TGGGGGAGGG GGAGCAAGAG GCTGCGCGCA GGTGGGCATC
    ATCCGGGCTC TGACGGAGTA CGGCATCCCC GTGGACATGG TTGGAGGGAC GTCCATCGGG
    GCCTTCATGG GCGCCCTGTA CTCGGAGGAA CGCAGCTACA GCCAGATCCG CATCCGGGCC
    AAGCAGTGGG CGGAGGACAT GATGTCGATG GTGAAGACCA TGCTGGACCT GACCTACCCC
    ATCACTTCCA TGTTCTCGGG GGCTGGCTTC AACAGCAGCA TCTCTAGCGT CTTCAAGGAC
    AGGCAGATCG AGGACCTGTG GATCCCATAC TTCGCCATCA CCACGGACAT CTCCGCATCT
    GCCATGCGGG TCCACACGGA CGGATCACTG TGGAGGTACG TGCGTGCCAG CATGTCCCTG
    TCGGGCTACA TGCCCCCACT CTGCGACCCC AAAGATGGGC ACCTGCTGAT GGATGGGGGC
    TACATCAACA ACCTCCCAGC GGATGTGGCC AGGTCAATGG GGGCAAAGGT GGTGATCGCC
    ATTGACGTGG GCAGCCGGGA TGAGACAGAC CTCACCAACT ACGGGGATGC GCTCTCGGGA
    TGGTGGCTCC TGTGGAAGCG CTGGAATCCC TTGGCCACGA AGGTCAAGGT GCTGAACATG
    GCTGAGATCC AGACACGCCT GGCCTACGTG TGCTGTGTCC GGCAGCTGGA GATGGTGAAG
    AGCAGCGACT ACTGCGAGTA CCTGCGCCCT CCGATTGACA GCTATGGCAC CCTGGAGTTC
    AGCAAGTTTG ATGAGATTTG TGAGGTGGGG TACCAGCATG GCCGGACAGT GTTTGACATC
    TGGGGTCGAA GCGGTGTGCT GGAGAAGATG CTGCAGGACC GGCAGGGGAC AAGCAAGATG
    AAGGCGTGCG ACGTCCTCAC CTGCCCCAAC GCCTCCTTCA CGGACCTTGC TGAGATTGTA
    TCACGTATTG AGCCTGCCAA GGTGGCTGCA GGGGACGATG AATCTGACTA CCAGACTGAG
    TATGAGGAGG TGCTGCTGGG AGGCCCCAAG GACGCGTACG TTGACTTCCA AAGCGCCCCA
    GCCGACCGGG GCTCCGACTC GGAGAATGAG CCCTCGCTTC GGCATCAACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53359
    Clone ID Related Accession (Same CDS sequence) XM_010810587.2 , XM_015465575.1
    Accession Version XM_015465575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product patatin-like phospholipase domain-containing protein 7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC ACGAGCATCC TCTTCGTGGT CTCGGGGCTG
    CTGCATGTGT ACCAGCGGAA GATTGACAGC GAGGAGGACA CGTTCCTGTT CATGGCACGG
    CCCGGTGAGA TGGTGGGCCA GCTGGCGGTG CTCACGGGCG AGCCCCTCAT CTTCACCATC
    AGGGCCAACC GGGACTGCAG CTTCCTGTCC ATCTCCAAGG CGCACTTCTA CGACATCATG
    CGGAGGCAGC CAGCCGTGGT CCTGAGCGTG GCGCACACCG TGGTGAGGCG GGTGTCCTCC
    TTCGTGCGGC AGATCGACTT CGCCCTGGAC TGGATGGAGG TGGAGGCCGG CCGCGCCATA
    TACAGGCAGG GGGACAAGTC CGACTGCACC TACATCGTGC TCAGCGGCCG CCTGCGCGCT
    GTGATCCAGA AGGACGACGG CAAGAAGCGC CTGGCGGGCG AGTACGGCCG CGGAGACCTC
    GTGGGCGTGG TGGAGACACT CACCCACCAG GCCAGGGCAA CCACAGTGCA TGCTATTCGG
    GACTCAGAGC TGGCCAAGCT TCCCGAAGGA GCCCTGAACT CCATCAAGCG CCAGTACCCA
    CAGGTTGTGA CTCGACTGAT TCGCCTCTTG GGCGAGAAGA TCCTGGGCAG CCTCCAGCAG
    GGAGCTGCGG CAGGTCACCA GTTTGGGTTC CATACTGTGG GCAGCAAGTG GGACTCGGGG
    AACGCCGCCA GCAACCTCTC CACAGTGGCC GTCATGCCAG TGTCGGAGGA CGTGCCCCTC
    ACCGCCTTCA CCCTGGAGCT CAAGCACGCC CTCAGCGCTA TTGGCCCTGC CCTGCTGCTG
    ACCAGTGACA GCATAAAACA GCGCCTTGGC TCTGCTGCTC TGGACAGTAT CCACGAGTAC
    CGGCTGTCCA GCTGGCTGGG GCAGCAGGAG GACATCCACC GGATCGTGCT CTACCAGGCG
    GACAGCACAC TCACGCCCTG GACCCAGCGC TGCATCCGGC AGGCAGACTG CATCCTCATC
    GTGGGCCTGG GCGAGCAGGA GCCCACGGTT GGCGAGCTGG AGCGGATGCT GGAGACTGCG
    GCCGTGCGTG CCCAGAAGCA GCTGGTGCTG CTGCACCGTC AGGACGGGCC CGCGCCGGCC
    CGCACCGCGG AGTGGCTCAA CATGCGGGGC TGGTGTTCTG GCCACCTGCA CCTCTGCTGC
    CCTCGCCGCG TCTTCTCCAG GAGGAGCCTG CCCAAGCTGG TGGAGCTGTA CGAGCGGGTT
    TGCCAGAGGC CCCCGGACCG GCACTCGGAC TTCTCCCGCC TGGCAAGAGT GCTGACCGGC
    AATGCCATCG CCTTGGTCCT TGGGGGAGGG GGAGCAAGAG GCTGCGCGCA GGTGGGCATC
    ATCCGGGCTC TGACGGAGTA CGGCATCCCC GTGGACATGG TTGGAGGGAC GTCCATCGGG
    GCCTTCATGG GCGCCCTGTA CTCGGAGGAA CGCAGCTACA GCCAGATCCG CATCCGGGCC
    AAGCAGTGGG CGGAGGACAT GATGTCGATG GTGAAGACCA TGCTGGACCT GACCTACCCC
    ATCACTTCCA TGTTCTCGGG GGCTGGCTTC AACAGCAGCA TCTCTAGCGT CTTCAAGGAC
    AGGCAGATCG AGGACCTGTG GATCCCATAC TTCGCCATCA CCACGGACAT CTCCGCATCT
    GCCATGCGGG TCCACACGGA CGGATCACTG TGGAGGTACG TGCGTGCCAG CATGTCCCTG
    TCGGGCTACA TGCCCCCACT CTGCGACCCC AAAGATGGGC ACCTGCTGAT GGATGGGGGC
    TACATCAACA ACCTCCCAGC GGATGTGGCC AGGTCAATGG GGGCAAAGGT GGTGATCGCC
    ATTGACGTGG GCAGCCGGGA TGAGACAGAC CTCACCAACT ACGGGGATGC GCTCTCGGGA
    TGGTGGCTCC TGTGGAAGCG CTGGAATCCC TTGGCCACGA AGGTCAAGGT GCTGAACATG
    GCTGAGATCC AGACACGCCT GGCCTACGTG TGCTGTGTCC GGCAGCTGGA GATGGTGAAG
    AGCAGCGACT ACTGCGAGTA CCTGCGCCCT CCGATTGACA GCTATGGCAC CCTGGAGTTC
    AGCAAGTTTG ATGAGATTTG TGAGGTGGGG TACCAGCATG GCCGGACAGT GTTTGACATC
    TGGGGTCGAA GCGGTGTGCT GGAGAAGATG CTGCAGGACC GGCAGGGGAC AAGCAAGATG
    AAGGCGTGCG ACGTCCTCAC CTGCCCCAAC GCCTCCTTCA CGGACCTTGC TGAGATTGTA
    TCACGTATTG AGCCTGCCAA GGTGGCTGCA GGGGACGATG AATCTGACTA CCAGACTGAG
    TATGAGGAGG TGCTGCTGGG AGGCCCCAAG GACGCGTACG TTGACTTCCA AAGCGCCCCA
    GCCGACCGGG GCTCCGACTC GGAGAATGAG CCCTCGCTTC GGCATCAACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321061.1
    CDS176..4069
    Translation

    Target ORF information:

    RefSeq Version XM_015465575.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGAT
    CGTGGGGGCA GCCGCGCCAC AGTCTGTGGT CCTCTGCCCA AGAGGAGCCA CTCCGTCCTG
    CTACCTTCTG TCTACGAAGA GGTGGCGGCT GAGCTCAGCC AGGCCCAGGC TGGCATCCAG
    GCCTCTTCGG CTCCTGCAGG GGCCACAGCA GGTGCCGCCT CAGATCTGAG GGTGGTGTGT
    GACCGAGCCC GGGTCCTGCC ACACACAGAG GAGCACCCCG GGGGCGCTGT GGCCAGCAAG
    CCCAAGAAGA GTGTGGTTGT TGCAGAGACC CCATCGGCTG TCCTCCCCAA CTCGGACGGT
    ACCTTGGACG AGTCTGTCAC CAGCAAGAAG ACAGACTCCA TCCTCAGAGC CGCCACACAG
    GACCTGCTCA CCCTGATGAA GCTGGATGAC ACGAGCATCC TCTTCGTGGT CTCGGGGCTG
    CTGCATGTGT ACCAGCGGAA GATTGACAGC GAGGAGGACA CGTTCCTGTT CATGGCACGG
    CCCGGTGAGA TGGTGGGCCA GCTGGCGGTG CTCACGGGCG AGCCCCTCAT CTTCACCATC
    AGGGCCAACC GGGACTGCAG CTTCCTGTCC ATCTCCAAGG CGCACTTCTA CGACATCATG
    CGGAGGCAGC CAGCCGTGGT CCTGAGCGTG GCGCACACCG TGGTGAGGCG GGTGTCCTCC
    TTCGTGCGGC AGATCGACTT CGCCCTGGAC TGGATGGAGG TGGAGGCCGG CCGCGCCATA
    TACAGGCAGG GGGACAAGTC CGACTGCACC TACATCGTGC TCAGCGGCCG CCTGCGCGCT
    GTGATCCAGA AGGACGACGG CAAGAAGCGC CTGGCGGGCG AGTACGGCCG CGGAGACCTC
    GTGGGCGTGG TGGAGACACT CACCCACCAG GCCAGGGCAA CCACAGTGCA TGCTATTCGG
    GACTCAGAGC TGGCCAAGCT TCCCGAAGGA GCCCTGAACT CCATCAAGCG CCAGTACCCA
    CAGGTTGTGA CTCGACTGAT TCGCCTCTTG GGCGAGAAGA TCCTGGGCAG CCTCCAGCAG
    GGAGCTGCGG CAGGTCACCA GTTTGGGTTC CATACTGTGG GCAGCAAGTG GGACTCGGGG
    AACGCCGCCA GCAACCTCTC CACAGTGGCC GTCATGCCAG TGTCGGAGGA CGTGCCCCTC
    ACCGCCTTCA CCCTGGAGCT CAAGCACGCC CTCAGCGCTA TTGGCCCTGC CCTGCTGCTG
    ACCAGTGACA GCATAAAACA GCGCCTTGGC TCTGCTGCTC TGGACAGTAT CCACGAGTAC
    CGGCTGTCCA GCTGGCTGGG GCAGCAGGAG GACATCCACC GGATCGTGCT CTACCAGGCG
    GACAGCACAC TCACGCCCTG GACCCAGCGC TGCATCCGGC AGGCAGACTG CATCCTCATC
    GTGGGCCTGG GCGAGCAGGA GCCCACGGTT GGCGAGCTGG AGCGGATGCT GGAGACTGCG
    GCCGTGCGTG CCCAGAAGCA GCTGGTGCTG CTGCACCGTC AGGACGGGCC CGCGCCGGCC
    CGCACCGCGG AGTGGCTCAA CATGCGGGGC TGGTGTTCTG GCCACCTGCA CCTCTGCTGC
    CCTCGCCGCG TCTTCTCCAG GAGGAGCCTG CCCAAGCTGG TGGAGCTGTA CGAGCGGGTT
    TGCCAGAGGC CCCCGGACCG GCACTCGGAC TTCTCCCGCC TGGCAAGAGT GCTGACCGGC
    AATGCCATCG CCTTGGTCCT TGGGGGAGGG GGAGCAAGAG GCTGCGCGCA GGTGGGCATC
    ATCCGGGCTC TGACGGAGTA CGGCATCCCC GTGGACATGG TTGGAGGGAC GTCCATCGGG
    GCCTTCATGG GCGCCCTGTA CTCGGAGGAA CGCAGCTACA GCCAGATCCG CATCCGGGCC
    AAGCAGTGGG CGGAGGACAT GATGTCGATG GTGAAGACCA TGCTGGACCT GACCTACCCC
    ATCACTTCCA TGTTCTCGGG GGCTGGCTTC AACAGCAGCA TCTCTAGCGT CTTCAAGGAC
    AGGCAGATCG AGGACCTGTG GATCCCATAC TTCGCCATCA CCACGGACAT CTCCGCATCT
    GCCATGCGGG TCCACACGGA CGGATCACTG TGGAGGTACG TGCGTGCCAG CATGTCCCTG
    TCGGGCTACA TGCCCCCACT CTGCGACCCC AAAGATGGGC ACCTGCTGAT GGATGGGGGC
    TACATCAACA ACCTCCCAGC GGATGTGGCC AGGTCAATGG GGGCAAAGGT GGTGATCGCC
    ATTGACGTGG GCAGCCGGGA TGAGACAGAC CTCACCAACT ACGGGGATGC GCTCTCGGGA
    TGGTGGCTCC TGTGGAAGCG CTGGAATCCC TTGGCCACGA AGGTCAAGGT GCTGAACATG
    GCTGAGATCC AGACACGCCT GGCCTACGTG TGCTGTGTCC GGCAGCTGGA GATGGTGAAG
    AGCAGCGACT ACTGCGAGTA CCTGCGCCCT CCGATTGACA GCTATGGCAC CCTGGAGTTC
    AGCAAGTTTG ATGAGATTTG TGAGGTGGGG TACCAGCATG GCCGGACAGT GTTTGACATC
    TGGGGTCGAA GCGGTGTGCT GGAGAAGATG CTGCAGGACC GGCAGGGGAC AAGCAAGATG
    AAGGCGTGCG ACGTCCTCAC CTGCCCCAAC GCCTCCTTCA CGGACCTTGC TGAGATTGTA
    TCACGTATTG AGCCTGCCAA GGTGGCTGCA GGGGACGATG AATCTGACTA CCAGACTGAG
    TATGAGGAGG TGCTGCTGGG AGGCCCCAAG GACGCGTACG TTGACTTCCA AAGCGCCCCA
    GCCGACCGGG GCTCCGACTC GGAGAATGAG CCCTCGCTTC GGCATCAACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53360
    Clone ID Related Accession (Same CDS sequence) XM_015465576.1 , XM_010810588.2
    Accession Version XM_015465576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product patatin-like phospholipase domain-containing protein 7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGGG
    GCCACAGCAG GTGCCGCCTC AGATCTGAGG GTGGTGTGTG ACCGAGCCCG GGTCCTGCCA
    CACACAGAGG AGCACCCCGG GGGCGCTGTG GCCAGCAAGC CCAAGAAGAG TGTGGTTGTT
    GCAGAGACCC CATCGGCTGT CCTCCCCAAC TCGGACGGTA CCTTGGACGA GTCTGTCACC
    AGCAAGAAGA CAGACTCCAT CCTCAGAGCC GCCACACAGG ACCTGCTCAC CCTGATGAAG
    CTGGATGACC CTTCGCTGTT AGATGGCCGG GTGGCGCTTC TCCACGTCCC AGGGGGCACG
    GTGGTGTTGA GGCAGGGAGA CCAGGACACG AGCATCCTCT TCGTGGTCTC GGGGCTGCTG
    CATGTGTACC AGCGGAAGAT TGACAGCGAG GAGGACACGT TCCTGTTCAT GGCACGGCCC
    GGTGAGATGG TGGGCCAGCT GGCGGTGCTC ACGGGCGAGC CCCTCATCTT CACCATCAGG
    GCCAACCGGG ACTGCAGCTT CCTGTCCATC TCCAAGGCGC ACTTCTACGA CATCATGCGG
    AGGCAGCCAG CCGTGGTCCT GAGCGTGGCG CACACCGTGG TGAGGCGGGT GTCCTCCTTC
    GTGCGGCAGA TCGACTTCGC CCTGGACTGG ATGGAGGTGG AGGCCGGCCG CGCCATATAC
    AGGCAGGGGG ACAAGTCCGA CTGCACCTAC ATCGTGCTCA GCGGCCGCCT GCGCGCTGTG
    ATCCAGAAGG ACGACGGCAA GAAGCGCCTG GCGGGCGAGT ACGGCCGCGG AGACCTCGTG
    GGCGTGGTGG AGACACTCAC CCACCAGGCC AGGGCAACCA CAGTGCATGC TATTCGGGAC
    TCAGAGCTGG CCAAGCTTCC CGAAGGAGCC CTGAACTCCA TCAAGCGCCA GTACCCACAG
    GTTGTGACTC GACTGATTCG CCTCTTGGGC GAGAAGATCC TGGGCAGCCT CCAGCAGGGA
    GCTGCGGCAG GTCACCAGTT TGGGTTCCAT ACTGTGGGCA GCAAGTGGGA CTCGGGGAAC
    GCCGCCAGCA ACCTCTCCAC AGTGGCCGTC ATGCCAGTGT CGGAGGACGT GCCCCTCACC
    GCCTTCACCC TGGAGCTCAA GCACGCCCTC AGCGCTATTG GCCCTGCCCT GCTGCTGACC
    AGTGACAGCA TAAAACAGCG CCTTGGCTCT GCTGCTCTGG ACAGTATCCA CGAGTACCGG
    CTGTCCAGCT GGCTGGGGCA GCAGGAGGAC ATCCACCGGA TCGTGCTCTA CCAGGCGGAC
    AGCACACTCA CGCCCTGGAC CCAGCGCTGC ATCCGGCAGG CAGACTGCAT CCTCATCGTG
    GGCCTGGGCG AGCAGGAGCC CACGGTTGGC GAGCTGGAGC GGATGCTGGA GACTGCGGCC
    GTGCGTGCCC AGAAGCAGCT GGTGCTGCTG CACCGTCAGG ACGGGCCCGC GCCGGCCCGC
    ACCGCGGAGT GGCTCAACAT GCGGGGCTGG TGTTCTGGCC ACCTGCACCT CTGCTGCCCT
    CGCCGCGTCT TCTCCAGGAG GAGCCTGCCC AAGCTGGTGG AGCTGTACGA GCGGGTTTGC
    CAGAGGCCCC CGGACCGGCA CTCGGACTTC TCCCGCCTGG CAAGAGTGCT GACCGGCAAT
    GCCATCGCCT TGGTCCTTGG GGGAGGGGGA GCAAGAGGCT GCGCGCAGGT GGGCATCATC
    CGGGCTCTGA CGGAGTACGG CATCCCCGTG GACATGGTTG GAGGGACGTC CATCGGGGCC
    TTCATGGGCG CCCTGTACTC GGAGGAACGC AGCTACAGCC AGATCCGCAT CCGGGCCAAG
    CAGTGGGCGG AGGACATGAT GTCGATGGTG AAGACCATGC TGGACCTGAC CTACCCCATC
    ACTTCCATGT TCTCGGGGGC TGGCTTCAAC AGCAGCATCT CTAGCGTCTT CAAGGACAGG
    CAGATCGAGG ACCTGTGGAT CCCATACTTC GCCATCACCA CGGACATCTC CGCATCTGCC
    ATGCGGGTCC ACACGGACGG ATCACTGTGG AGGTACGTGC GTGCCAGCAT GTCCCTGTCG
    GGCTACATGC CCCCACTCTG CGACCCCAAA GATGGGCACC TGCTGATGGA TGGGGGCTAC
    ATCAACAACC TCCCAGCGGA TGTGGCCAGG TCAATGGGGG CAAAGGTGGT GATCGCCATT
    GACGTGGGCA GCCGGGATGA GACAGACCTC ACCAACTACG GGGATGCGCT CTCGGGATGG
    TGGCTCCTGT GGAAGCGCTG GAATCCCTTG GCCACGAAGG TCAAGGTGCT GAACATGGCT
    GAGATCCAGA CACGCCTGGC CTACGTGTGC TGTGTCCGGC AGCTGGAGAT GGTGAAGAGC
    AGCGACTACT GCGAGTACCT GCGCCCTCCG ATTGACAGCT ATGGCACCCT GGAGTTCAGC
    AAGTTTGATG AGATTTGTGA GGTGGGGTAC CAGCATGGCC GGACAGTGTT TGACATCTGG
    GGTCGAAGCG GTGTGCTGGA GAAGATGCTG CAGGACCGGC AGGGGACAAG CAAGATGAAG
    GCGTGCGACG TCCTCACCTG CCCCAACGCC TCCTTCACGG ACCTTGCTGA GATTGTATCA
    CGTATTGAGC CTGCCAAGGT GGCTGCAGGG GACGATGAAT CTGACTACCA GACTGAGTAT
    GAGGAGGTGC TGCTGGGAGG CCCCAAGGAC GCGTACGTTG ACTTCCAAAG CGCCCCAGCC
    GACCGGGGCT CCGACTCGGA GAATGAGCCC TCGCTTCGGC ATCAACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321062.1
    CDS176..4006
    Translation

    Target ORF information:

    RefSeq Version XM_015465576.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGGG
    GCCACAGCAG GTGCCGCCTC AGATCTGAGG GTGGTGTGTG ACCGAGCCCG GGTCCTGCCA
    CACACAGAGG AGCACCCCGG GGGCGCTGTG GCCAGCAAGC CCAAGAAGAG TGTGGTTGTT
    GCAGAGACCC CATCGGCTGT CCTCCCCAAC TCGGACGGTA CCTTGGACGA GTCTGTCACC
    AGCAAGAAGA CAGACTCCAT CCTCAGAGCC GCCACACAGG ACCTGCTCAC CCTGATGAAG
    CTGGATGACC CTTCGCTGTT AGATGGCCGG GTGGCGCTTC TCCACGTCCC AGGGGGCACG
    GTGGTGTTGA GGCAGGGAGA CCAGGACACG AGCATCCTCT TCGTGGTCTC GGGGCTGCTG
    CATGTGTACC AGCGGAAGAT TGACAGCGAG GAGGACACGT TCCTGTTCAT GGCACGGCCC
    GGTGAGATGG TGGGCCAGCT GGCGGTGCTC ACGGGCGAGC CCCTCATCTT CACCATCAGG
    GCCAACCGGG ACTGCAGCTT CCTGTCCATC TCCAAGGCGC ACTTCTACGA CATCATGCGG
    AGGCAGCCAG CCGTGGTCCT GAGCGTGGCG CACACCGTGG TGAGGCGGGT GTCCTCCTTC
    GTGCGGCAGA TCGACTTCGC CCTGGACTGG ATGGAGGTGG AGGCCGGCCG CGCCATATAC
    AGGCAGGGGG ACAAGTCCGA CTGCACCTAC ATCGTGCTCA GCGGCCGCCT GCGCGCTGTG
    ATCCAGAAGG ACGACGGCAA GAAGCGCCTG GCGGGCGAGT ACGGCCGCGG AGACCTCGTG
    GGCGTGGTGG AGACACTCAC CCACCAGGCC AGGGCAACCA CAGTGCATGC TATTCGGGAC
    TCAGAGCTGG CCAAGCTTCC CGAAGGAGCC CTGAACTCCA TCAAGCGCCA GTACCCACAG
    GTTGTGACTC GACTGATTCG CCTCTTGGGC GAGAAGATCC TGGGCAGCCT CCAGCAGGGA
    GCTGCGGCAG GTCACCAGTT TGGGTTCCAT ACTGTGGGCA GCAAGTGGGA CTCGGGGAAC
    GCCGCCAGCA ACCTCTCCAC AGTGGCCGTC ATGCCAGTGT CGGAGGACGT GCCCCTCACC
    GCCTTCACCC TGGAGCTCAA GCACGCCCTC AGCGCTATTG GCCCTGCCCT GCTGCTGACC
    AGTGACAGCA TAAAACAGCG CCTTGGCTCT GCTGCTCTGG ACAGTATCCA CGAGTACCGG
    CTGTCCAGCT GGCTGGGGCA GCAGGAGGAC ATCCACCGGA TCGTGCTCTA CCAGGCGGAC
    AGCACACTCA CGCCCTGGAC CCAGCGCTGC ATCCGGCAGG CAGACTGCAT CCTCATCGTG
    GGCCTGGGCG AGCAGGAGCC CACGGTTGGC GAGCTGGAGC GGATGCTGGA GACTGCGGCC
    GTGCGTGCCC AGAAGCAGCT GGTGCTGCTG CACCGTCAGG ACGGGCCCGC GCCGGCCCGC
    ACCGCGGAGT GGCTCAACAT GCGGGGCTGG TGTTCTGGCC ACCTGCACCT CTGCTGCCCT
    CGCCGCGTCT TCTCCAGGAG GAGCCTGCCC AAGCTGGTGG AGCTGTACGA GCGGGTTTGC
    CAGAGGCCCC CGGACCGGCA CTCGGACTTC TCCCGCCTGG CAAGAGTGCT GACCGGCAAT
    GCCATCGCCT TGGTCCTTGG GGGAGGGGGA GCAAGAGGCT GCGCGCAGGT GGGCATCATC
    CGGGCTCTGA CGGAGTACGG CATCCCCGTG GACATGGTTG GAGGGACGTC CATCGGGGCC
    TTCATGGGCG CCCTGTACTC GGAGGAACGC AGCTACAGCC AGATCCGCAT CCGGGCCAAG
    CAGTGGGCGG AGGACATGAT GTCGATGGTG AAGACCATGC TGGACCTGAC CTACCCCATC
    ACTTCCATGT TCTCGGGGGC TGGCTTCAAC AGCAGCATCT CTAGCGTCTT CAAGGACAGG
    CAGATCGAGG ACCTGTGGAT CCCATACTTC GCCATCACCA CGGACATCTC CGCATCTGCC
    ATGCGGGTCC ACACGGACGG ATCACTGTGG AGGTACGTGC GTGCCAGCAT GTCCCTGTCG
    GGCTACATGC CCCCACTCTG CGACCCCAAA GATGGGCACC TGCTGATGGA TGGGGGCTAC
    ATCAACAACC TCCCAGCGGA TGTGGCCAGG TCAATGGGGG CAAAGGTGGT GATCGCCATT
    GACGTGGGCA GCCGGGATGA GACAGACCTC ACCAACTACG GGGATGCGCT CTCGGGATGG
    TGGCTCCTGT GGAAGCGCTG GAATCCCTTG GCCACGAAGG TCAAGGTGCT GAACATGGCT
    GAGATCCAGA CACGCCTGGC CTACGTGTGC TGTGTCCGGC AGCTGGAGAT GGTGAAGAGC
    AGCGACTACT GCGAGTACCT GCGCCCTCCG ATTGACAGCT ATGGCACCCT GGAGTTCAGC
    AAGTTTGATG AGATTTGTGA GGTGGGGTAC CAGCATGGCC GGACAGTGTT TGACATCTGG
    GGTCGAAGCG GTGTGCTGGA GAAGATGCTG CAGGACCGGC AGGGGACAAG CAAGATGAAG
    GCGTGCGACG TCCTCACCTG CCCCAACGCC TCCTTCACGG ACCTTGCTGA GATTGTATCA
    CGTATTGAGC CTGCCAAGGT GGCTGCAGGG GACGATGAAT CTGACTACCA GACTGAGTAT
    GAGGAGGTGC TGCTGGGAGG CCCCAAGGAC GCGTACGTTG ACTTCCAAAG CGCCCCAGCC
    GACCGGGGCT CCGACTCGGA GAATGAGCCC TCGCTTCGGC ATCAACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53360
    Clone ID Related Accession (Same CDS sequence) XM_015465576.1 , XM_010810588.2
    Accession Version XM_010810588.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product patatin-like phospholipase domain-containing protein 7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104304.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGGG
    GCCACAGCAG GTGCCGCCTC AGATCTGAGG GTGGTGTGTG ACCGAGCCCG GGTCCTGCCA
    CACACAGAGG AGCACCCCGG GGGCGCTGTG GCCAGCAAGC CCAAGAAGAG TGTGGTTGTT
    GCAGAGACCC CATCGGCTGT CCTCCCCAAC TCGGACGGTA CCTTGGACGA GTCTGTCACC
    AGCAAGAAGA CAGACTCCAT CCTCAGAGCC GCCACACAGG ACCTGCTCAC CCTGATGAAG
    CTGGATGACC CTTCGCTGTT AGATGGCCGG GTGGCGCTTC TCCACGTCCC AGGGGGCACG
    GTGGTGTTGA GGCAGGGAGA CCAGGACACG AGCATCCTCT TCGTGGTCTC GGGGCTGCTG
    CATGTGTACC AGCGGAAGAT TGACAGCGAG GAGGACACGT TCCTGTTCAT GGCACGGCCC
    GGTGAGATGG TGGGCCAGCT GGCGGTGCTC ACGGGCGAGC CCCTCATCTT CACCATCAGG
    GCCAACCGGG ACTGCAGCTT CCTGTCCATC TCCAAGGCGC ACTTCTACGA CATCATGCGG
    AGGCAGCCAG CCGTGGTCCT GAGCGTGGCG CACACCGTGG TGAGGCGGGT GTCCTCCTTC
    GTGCGGCAGA TCGACTTCGC CCTGGACTGG ATGGAGGTGG AGGCCGGCCG CGCCATATAC
    AGGCAGGGGG ACAAGTCCGA CTGCACCTAC ATCGTGCTCA GCGGCCGCCT GCGCGCTGTG
    ATCCAGAAGG ACGACGGCAA GAAGCGCCTG GCGGGCGAGT ACGGCCGCGG AGACCTCGTG
    GGCGTGGTGG AGACACTCAC CCACCAGGCC AGGGCAACCA CAGTGCATGC TATTCGGGAC
    TCAGAGCTGG CCAAGCTTCC CGAAGGAGCC CTGAACTCCA TCAAGCGCCA GTACCCACAG
    GTTGTGACTC GACTGATTCG CCTCTTGGGC GAGAAGATCC TGGGCAGCCT CCAGCAGGGA
    GCTGCGGCAG GTCACCAGTT TGGGTTCCAT ACTGTGGGCA GCAAGTGGGA CTCGGGGAAC
    GCCGCCAGCA ACCTCTCCAC AGTGGCCGTC ATGCCAGTGT CGGAGGACGT GCCCCTCACC
    GCCTTCACCC TGGAGCTCAA GCACGCCCTC AGCGCTATTG GCCCTGCCCT GCTGCTGACC
    AGTGACAGCA TAAAACAGCG CCTTGGCTCT GCTGCTCTGG ACAGTATCCA CGAGTACCGG
    CTGTCCAGCT GGCTGGGGCA GCAGGAGGAC ATCCACCGGA TCGTGCTCTA CCAGGCGGAC
    AGCACACTCA CGCCCTGGAC CCAGCGCTGC ATCCGGCAGG CAGACTGCAT CCTCATCGTG
    GGCCTGGGCG AGCAGGAGCC CACGGTTGGC GAGCTGGAGC GGATGCTGGA GACTGCGGCC
    GTGCGTGCCC AGAAGCAGCT GGTGCTGCTG CACCGTCAGG ACGGGCCCGC GCCGGCCCGC
    ACCGCGGAGT GGCTCAACAT GCGGGGCTGG TGTTCTGGCC ACCTGCACCT CTGCTGCCCT
    CGCCGCGTCT TCTCCAGGAG GAGCCTGCCC AAGCTGGTGG AGCTGTACGA GCGGGTTTGC
    CAGAGGCCCC CGGACCGGCA CTCGGACTTC TCCCGCCTGG CAAGAGTGCT GACCGGCAAT
    GCCATCGCCT TGGTCCTTGG GGGAGGGGGA GCAAGAGGCT GCGCGCAGGT GGGCATCATC
    CGGGCTCTGA CGGAGTACGG CATCCCCGTG GACATGGTTG GAGGGACGTC CATCGGGGCC
    TTCATGGGCG CCCTGTACTC GGAGGAACGC AGCTACAGCC AGATCCGCAT CCGGGCCAAG
    CAGTGGGCGG AGGACATGAT GTCGATGGTG AAGACCATGC TGGACCTGAC CTACCCCATC
    ACTTCCATGT TCTCGGGGGC TGGCTTCAAC AGCAGCATCT CTAGCGTCTT CAAGGACAGG
    CAGATCGAGG ACCTGTGGAT CCCATACTTC GCCATCACCA CGGACATCTC CGCATCTGCC
    ATGCGGGTCC ACACGGACGG ATCACTGTGG AGGTACGTGC GTGCCAGCAT GTCCCTGTCG
    GGCTACATGC CCCCACTCTG CGACCCCAAA GATGGGCACC TGCTGATGGA TGGGGGCTAC
    ATCAACAACC TCCCAGCGGA TGTGGCCAGG TCAATGGGGG CAAAGGTGGT GATCGCCATT
    GACGTGGGCA GCCGGGATGA GACAGACCTC ACCAACTACG GGGATGCGCT CTCGGGATGG
    TGGCTCCTGT GGAAGCGCTG GAATCCCTTG GCCACGAAGG TCAAGGTGCT GAACATGGCT
    GAGATCCAGA CACGCCTGGC CTACGTGTGC TGTGTCCGGC AGCTGGAGAT GGTGAAGAGC
    AGCGACTACT GCGAGTACCT GCGCCCTCCG ATTGACAGCT ATGGCACCCT GGAGTTCAGC
    AAGTTTGATG AGATTTGTGA GGTGGGGTAC CAGCATGGCC GGACAGTGTT TGACATCTGG
    GGTCGAAGCG GTGTGCTGGA GAAGATGCTG CAGGACCGGC AGGGGACAAG CAAGATGAAG
    GCGTGCGACG TCCTCACCTG CCCCAACGCC TCCTTCACGG ACCTTGCTGA GATTGTATCA
    CGTATTGAGC CTGCCAAGGT GGCTGCAGGG GACGATGAAT CTGACTACCA GACTGAGTAT
    GAGGAGGTGC TGCTGGGAGG CCCCAAGGAC GCGTACGTTG ACTTCCAAAG CGCCCCAGCC
    GACCGGGGCT CCGACTCGGA GAATGAGCCC TCGCTTCGGC ATCAACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808890.1
    CDS176..4006
    Translation

    Target ORF information:

    RefSeq Version XM_010810588.2
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 7 (PNPLA7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810588.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCAAG AGGAGGACAC TGGCCCAGAG GTGGGCTTCT ATCTGGCCAC CCTATGGTCC 
    TGGGGTCTGG AGCTGATGGA GCAGCATTCC CAGTCCACCA TGCTGCTGGG GATTGTGATT
    GGAGTCCTCC TGGCCCTGGC CCTCATTGGC ACCACCATCT TTTTTGTGTA CAGAAGGGTT
    AGCCAATTCC GACAGTTGCA GCCCAGTCCT CAGTACAGGT TCCGGAAGAG AGACAAGGTG
    ATGTTCTACG GCAGGAAGAT CATGAGGAAG GTGACCACGC TTCCCCACAC ACTTGTGGGG
    AACACAGCTG CTCCCCGACA GCGGGTGAGG AAGAGGACCA AGGTGCTATC TTTGGCCAAG
    AGGATCCTGC GGTTCAAGAA GGAATACCCT ACCTTGCAGC CAAAGGAGCC CCCTCCCTCC
    CTGCTGGAGG CTGACCTCAC AGAGTTTGAT GTGAAGAATT CTCACCTGCC GTCAGAAGTT
    CTGTACATGC TGAAAAACGT CCGGGTCCTG GGTCACTTTG AGAAGCCGCT ATTCCTGGAA
    CTTTGCAAAC ACATGGTCTT TGTGCAGCTT CTGGAAGGGG AGTACATCTT CCGGCCGGGG
    GAGCTGGACA ACAGCATCTA TGTGGTGCAG GACGGGCGGC TGGAGGTCCG CGTCCAGGAC
    TCGGCGCAGG ACGGCACTGA GGTGACAGTC AAAGAGGTTC TGGCTGGAGA CAGCGTCCAC
    AGCCTCCTCA GCATTCTGGA CGTCATCACC GGCCACGCCG CGCCGTACAA AACTGTGTCC
    GCCCGCGCTG CCGTCCCCTC CACCATCCTC CGGCTCCCGG CTACGGCTTT TCAAGGAGTG
    TTTGAGAGAT ACCCTGAAAC GCTGGTGAGG GTGGTGCAGA TCATCATGGT GCGGCTGCAG
    AGGGTCACCT TTCTGGCTCT GCACAACTAC CTGGGCCTGA CCACGGAGCT CTTCAATCCC
    GAGAGCCAGG CCATCCCGCT CATGTCTGTG GCCAGCGTGA CTGCCGGGAA GGCCAAGAAG
    CCGACGTGCT GCAATCTGGA GGAGAGGCCG CCTCGGCCCC AGGAGCCCTG CGGCCCAGGG
    GCCACAGCAG GTGCCGCCTC AGATCTGAGG GTGGTGTGTG ACCGAGCCCG GGTCCTGCCA
    CACACAGAGG AGCACCCCGG GGGCGCTGTG GCCAGCAAGC CCAAGAAGAG TGTGGTTGTT
    GCAGAGACCC CATCGGCTGT CCTCCCCAAC TCGGACGGTA CCTTGGACGA GTCTGTCACC
    AGCAAGAAGA CAGACTCCAT CCTCAGAGCC GCCACACAGG ACCTGCTCAC CCTGATGAAG
    CTGGATGACC CTTCGCTGTT AGATGGCCGG GTGGCGCTTC TCCACGTCCC AGGGGGCACG
    GTGGTGTTGA GGCAGGGAGA CCAGGACACG AGCATCCTCT TCGTGGTCTC GGGGCTGCTG
    CATGTGTACC AGCGGAAGAT TGACAGCGAG GAGGACACGT TCCTGTTCAT GGCACGGCCC
    GGTGAGATGG TGGGCCAGCT GGCGGTGCTC ACGGGCGAGC CCCTCATCTT CACCATCAGG
    GCCAACCGGG ACTGCAGCTT CCTGTCCATC TCCAAGGCGC ACTTCTACGA CATCATGCGG
    AGGCAGCCAG CCGTGGTCCT GAGCGTGGCG CACACCGTGG TGAGGCGGGT GTCCTCCTTC
    GTGCGGCAGA TCGACTTCGC CCTGGACTGG ATGGAGGTGG AGGCCGGCCG CGCCATATAC
    AGGCAGGGGG ACAAGTCCGA CTGCACCTAC ATCGTGCTCA GCGGCCGCCT GCGCGCTGTG
    ATCCAGAAGG ACGACGGCAA GAAGCGCCTG GCGGGCGAGT ACGGCCGCGG AGACCTCGTG
    GGCGTGGTGG AGACACTCAC CCACCAGGCC AGGGCAACCA CAGTGCATGC TATTCGGGAC
    TCAGAGCTGG CCAAGCTTCC CGAAGGAGCC CTGAACTCCA TCAAGCGCCA GTACCCACAG
    GTTGTGACTC GACTGATTCG CCTCTTGGGC GAGAAGATCC TGGGCAGCCT CCAGCAGGGA
    GCTGCGGCAG GTCACCAGTT TGGGTTCCAT ACTGTGGGCA GCAAGTGGGA CTCGGGGAAC
    GCCGCCAGCA ACCTCTCCAC AGTGGCCGTC ATGCCAGTGT CGGAGGACGT GCCCCTCACC
    GCCTTCACCC TGGAGCTCAA GCACGCCCTC AGCGCTATTG GCCCTGCCCT GCTGCTGACC
    AGTGACAGCA TAAAACAGCG CCTTGGCTCT GCTGCTCTGG ACAGTATCCA CGAGTACCGG
    CTGTCCAGCT GGCTGGGGCA GCAGGAGGAC ATCCACCGGA TCGTGCTCTA CCAGGCGGAC
    AGCACACTCA CGCCCTGGAC CCAGCGCTGC ATCCGGCAGG CAGACTGCAT CCTCATCGTG
    GGCCTGGGCG AGCAGGAGCC CACGGTTGGC GAGCTGGAGC GGATGCTGGA GACTGCGGCC
    GTGCGTGCCC AGAAGCAGCT GGTGCTGCTG CACCGTCAGG ACGGGCCCGC GCCGGCCCGC
    ACCGCGGAGT GGCTCAACAT GCGGGGCTGG TGTTCTGGCC ACCTGCACCT CTGCTGCCCT
    CGCCGCGTCT TCTCCAGGAG GAGCCTGCCC AAGCTGGTGG AGCTGTACGA GCGGGTTTGC
    CAGAGGCCCC CGGACCGGCA CTCGGACTTC TCCCGCCTGG CAAGAGTGCT GACCGGCAAT
    GCCATCGCCT TGGTCCTTGG GGGAGGGGGA GCAAGAGGCT GCGCGCAGGT GGGCATCATC
    CGGGCTCTGA CGGAGTACGG CATCCCCGTG GACATGGTTG GAGGGACGTC CATCGGGGCC
    TTCATGGGCG CCCTGTACTC GGAGGAACGC AGCTACAGCC AGATCCGCAT CCGGGCCAAG
    CAGTGGGCGG AGGACATGAT GTCGATGGTG AAGACCATGC TGGACCTGAC CTACCCCATC
    ACTTCCATGT TCTCGGGGGC TGGCTTCAAC AGCAGCATCT CTAGCGTCTT CAAGGACAGG
    CAGATCGAGG ACCTGTGGAT CCCATACTTC GCCATCACCA CGGACATCTC CGCATCTGCC
    ATGCGGGTCC ACACGGACGG ATCACTGTGG AGGTACGTGC GTGCCAGCAT GTCCCTGTCG
    GGCTACATGC CCCCACTCTG CGACCCCAAA GATGGGCACC TGCTGATGGA TGGGGGCTAC
    ATCAACAACC TCCCAGCGGA TGTGGCCAGG TCAATGGGGG CAAAGGTGGT GATCGCCATT
    GACGTGGGCA GCCGGGATGA GACAGACCTC ACCAACTACG GGGATGCGCT CTCGGGATGG
    TGGCTCCTGT GGAAGCGCTG GAATCCCTTG GCCACGAAGG TCAAGGTGCT GAACATGGCT
    GAGATCCAGA CACGCCTGGC CTACGTGTGC TGTGTCCGGC AGCTGGAGAT GGTGAAGAGC
    AGCGACTACT GCGAGTACCT GCGCCCTCCG ATTGACAGCT ATGGCACCCT GGAGTTCAGC
    AAGTTTGATG AGATTTGTGA GGTGGGGTAC CAGCATGGCC GGACAGTGTT TGACATCTGG
    GGTCGAAGCG GTGTGCTGGA GAAGATGCTG CAGGACCGGC AGGGGACAAG CAAGATGAAG
    GCGTGCGACG TCCTCACCTG CCCCAACGCC TCCTTCACGG ACCTTGCTGA GATTGTATCA
    CGTATTGAGC CTGCCAAGGT GGCTGCAGGG GACGATGAAT CTGACTACCA GACTGAGTAT
    GAGGAGGTGC TGCTGGGAGG CCCCAAGGAC GCGTACGTTG ACTTCCAAAG CGCCCCAGCC
    GACCGGGGCT CCGACTCGGA GAATGAGCCC TCGCTTCGGC ATCAACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.