Nlrp5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlrp5
Entrez Gene ID	23968
Full Name	NLR family, pyrin domain containing 5
Synonyms	Mater,Nalp5,Op1,PAN11
General protein information	Preferred Names NLR family, pyrin domain containing 5 Names NACHT, LRR and PYD domains-containing protein 5 NACHT, leucine rich repeat and PYD containing 5 maternal antigen that embryos require ooplasm-specific protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 A3\|7 10.22 cM
Summary	This gene encodes a member of the NACHT, leucine-rich repeat, and pyrin domain containing family. Members of this family have a pyrin domain at the N-terminus, a central NACHT domain, and a C-terminal leucine-rich repeat domain. This gene encodes a maternal-effect factor that is essential for early embryonic development in the mouse. Homozygous null mutant females are sterile, and embryos die following the first cleavage. This gene is required for endoplasmic reticulum redistribution and calcium homeostasis in oocytes. In addition, ovulated oocytes mutant for this gene have abnormal mitochondrial localization and increased mitochondrial activity, which results in mitochondrial damage and early embryonic lethality. Pseudogenes of this gene have been found on chromosomes 7 and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_011860.3	NP_035990.1	NACHT, LRR and PYD domains-containing protein 5 isoform a
NM_001039143.2	NP_001034232.1	NACHT, LRR and PYD domains-containing protein 5 isoform b
NM_001305857.1	NP_001292786.1	NACHT, LRR and PYD domains-containing protein 5 isoform c

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NLRP5	XP_001139299.2
	NLRP5	NP_001121103.1
Canis lupus familiaris (dog)	NLRP5	XP_005616898.1
Bos taurus (cattle)	NLRP5	NP_001007815.1
Rattus norvegicus (Norway rat)	Nlrp5	NP_001100944.1
Homo sapiens (human)	NLRP5	NP_703148.4
Mus musculus (house mouse)	Nlrp5	NP_035990.1

Gene Ontology
Bibliography

Related articles in PubMed

The role of MATER in endoplasmic reticulum distribution and calcium homeostasis in mouse oocytes.
Kim B, Zhang X, Kan R, Cohen R, Mukai C, Travis AJ, Coonrod SA
Developmental biology386(2)331-9(2014 Feb)

NLRP5 mediates mitochondrial function in mouse oocytes and embryos.
Fernandes R, Tsuda C, Perumalsamy AL, Naranian T, Chong J, Acton BM, Tong ZB, Nelson LM, Jurisicova A
Biology of reproduction86(5)138, 1-10(2012 May)

Autoimmune oophoritis with multiple molecular targets mitigated by transgenic expression of mater.
Otsuka N, Tong ZB, Vanevski K, Tu W, Cheng MH, Nelson LM
Endocrinology152(6)2465-73(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression of Mater protein is decreased in mature oocytes from old versus young mice.
Title: Decrease in expression of maternal effect gene Mater is associated with maternal ageing in mice.

MATER is part of the subcortical maternal complex (SCMC). SCMC is required for formation of the cytoplasmic F-actin meshwork that controls the central position of the spindle and ensures symmetric division.
Title: The subcortical maternal complex controls symmetric division of mouse zygotes by regulating F-actin dynamics.

MATER is required for endoplasmic reticulum distribution and Ca2+ homeostasis in oocytes, likely due to defects in lattice-mediated ER positioning and/or redistribution.
Title: The role of MATER in endoplasmic reticulum distribution and calcium homeostasis in mouse oocytes.

It was concluded that lack of Nlrp5 in oocytes triggers premature activation of the mitochondrial pool, causing mitochondrial damage that cannot be rescued by inactivation of Bax.
Title: NLRP5 mediates mitochondrial function in mouse oocytes and embryos.

Transgenic expression of MATER induces antigen-specific tolerance with a significant reduction in ovarian autoimmunity.
Title: Autoimmune oophoritis with multiple molecular targets mitigated by transgenic expression of mater.

The following Nlrp5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlrp5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15892	NM_001039143.2 Latest version!	Mus musculus NLR family, pyrin domain containing 5 (Nlrp5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OMu16213	NM_011860.3 Latest version!	Mus musculus NLR family, pyrin domain containing 5 (Nlrp5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OMu69157	NM_001305857.1 Latest version!	Mus musculus NLR family, pyrin domain containing 5 (Nlrp5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlrp5 ( NM_001039143.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034232.1 Mus musculus NLR family, pyrin domain containing 5 (Nlrp5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15892
Clone ID Related Accession (Same CDS sequence)	NM_001039143.2
Accession Version	NM_001039143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 5 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK087774.1, BC053384.1 and AC159288.4. On Apr 4, 2015 this sequence version replaced NM_001039143.1. Transcript Variant: This variant (2) lacks an exon in the 5' coding region compared to variant 1. It encodes isoform b, which is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK087774.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849384 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCCTC CAGAAAAAGA AAGTAAAGCA ATCTTGAAAG CACGTGGATT GGAAGAGGAA 
CAGAAGTCAG AAAGAAAAAT GACTTCTCCA GAAAACGACA GTAAATCAAT CCAGAAAGAC 
CAAGGACCAG AGCAGGAGCA GACATCAGAA AGCACAATGG GTCCTCCAGA AAAAGACAGT 
AAAGCAATCT TGAAAGCACG TGGATTGGAA GAGGAACAGA AGTCAGAAAG CACAATGTCT 
CCTTCAGAAA ATGTCAGTAG AGCAATCCTG AAAGACAGTG GATCAGAAGA AGTGGAACAG 
GCGTCAGAAA GAAAAATGAC TTCTCCAGAA AACGACAGTA AATCAATCCA GAAAGACCAA 
GGACCAGAGC AGGAGCAGAC ATCAGATAAT GGAGGTGACT TACAAGACTA CAAGGCCCAT 
GTGATTGCTA AGTTCGACAC AAGTGTGGAT CTACACTATG ACAGCCCAGA GATGAAATTA 
TTGTCTGATG CTTTTAAACC ATACCAGAAA ACCTTCCAGC CTCACACCAT TATCCTACAT 
GGAAGACCAG GAGTTGGGAA GTCAGCTTTG GCCAGAAGTA TTGTTCTTGG CTGGGCACAG 
GGTAAACTCT TCCAAAAAAT GTCCTTTGTC ATCTTCTTCT CTGTTAGAGA AATAAAGTGG 
ACAGAGAAGA GCAGTTTGGC ACAGCTGATT GCTAAGGAGT GTCCAGACTC CTGGGATCTA 
GTGACAAAGA TCATGTCCCA ACCAGAAAGA CTCTTGTTTG TCATAGATGG CTTGGATGAT 
ATGGACTCTG TCCTCCAACA TGATGATATG ACACTATCCA GAGACTGGAA GGATGAACAG 
CCCATATACA TCCTGATGTA CAGCCTCCTG AGGAAGGCTC TCCTACCTCA GTCCTTTCTC 
ATCATTACCA CCAGAAACAC AGGCTTAGAA AAACTCAAGT CAATGGTTGT GTCCCCCCTC 
TATATACTGG TTGAAGGACT GTCTGCATCA AGGAGATCTC AGCTGGTCCT CGAGAACATC 
TCCAATGAGT CTGATAGAAT ACAAGTCTTC CATTCTCTGA TAGAAAATCA CCAGCTGTTT 
GACCAATGCC AGGCCCCCTC TGTGTGCTCC CTGGTCTGTG AGGCTCTACA GCTACAGAAG 
AAACTGGGAA AGAGATGCAC CCTACCCTGC CAGACTCTCA CCGGTTTGTA TGCCACGTTG 
GTGTTTCACC AGCTCACCTT GAAAAGGCCT TCCCAGAGCG CTCTCAGTCA GGAAGAACAG 
ATTACTCTAG TGGGTTTGTG CATGATGGCA GCTGAAGGAG TGTGGACCAT GAGGTCGGTG 
TTCTATGATG ATGACCTGAA GAACTATAGC CTAAAGGAGT CTGAGATCTT GGCCCTCTTT 
CACATGAACA TCCTTCTCCA GGTTGGCCAC AACAGTGAGC AGTGTTATGT TTTCTCCCAC 
CTCAGCCTGC AGGATTTCTT TGCTGCCTTA TATTATGTTT TAGAAGGGCT GGAGGAATGG 
AATCAGCATT TTTGCTTCAT TGAAAACCAA AGGAGCATCA TGGAGGTGAA GAGAACTGAC 
GACACTCGCC TCCTCGGGAT GAAGCGTTTC TTATTTGGCC TCATGAACAA GGATATCTTG 
AAGACTCTGG AGGTTCTGTT TGAATATCCC GTGATTCCAA CTGTTGAGCA GAAGCTCCAA 
CACTGGGTCT CTCTGATAGC TCAGCAGGTC AATGGCACCA GCCCAATGGA CACCCTGGAT 
GCCTTCTATT GTCTATTTGA GTCTCAGGAT GAAGAGTTTG TTGGCGGGGC TCTCAAACGC 
TTCCAAGAAG TGTGGCTGCT GATTAACCAG AAGATGGACT TGAAGGTCTC TTCCTACTGT 
CTCAAGCACT GTCAGAACTT GAAGGCAATC CGGGTGGATA TCAGAGACCT CCTCTCGGTA 
GATAATACTC TCGAGCTGTG CCCTGTTGTT ACTGTCCAGG AGACACAATG TAAGCCCCTC 
CTCATGGAGT GGTGGGGAAA CTTCTGCTCT GTGCTTGGCA GCCTCCGGAA CTTGAAGGAG 
CTGGACTTGG GCGACAGCAT CCTGAGTCAA CGGGCCATGA AGATACTGTG CCTCGAGCTG 
CGGAATCAGT CCTGCAGAAT ACAGAAGCTG ACGTTTAAGA GTGCAGAGGT AGTGTCTGGC 
CTGAAACATC TCTGGAAGCT CCTTTTTAGC AATCAAAACT TAAAGTACCT CAATCTAGGG 
AACACTCCCA TGAAGGATGA TGACATGAAG TTAGCCTGCG AAGCGCTGAA ACATCCAAAG 
TGCTCCGTGG AGACTCTGAG GTTGGATTCC TGTGAGTTAA CCATCATTGG TTATGAGATG 
ATCTCCACGC TTCTTATTTC AACCACCAGG CTAAAGTGTC TCAGCCTGGC CAAAAATAGA 
GTGGGAGTAA AAAGCATGAT ATCCCTTGGG AATGCCTTGA GTAGCTCAAT GTGTCTACTG 
CAAAAGTTGA TACTGGACAA CTGTGGCCTC ACACCTGCCA GCTGCCACCT TCTGGTCTCA 
GCCCTTTTCA GCAACCAGAA CTTGACACAC CTGTGCCTGT CAAACAACAG CCTGGGGACT 
GAAGGAGTGC AACAGCTGTG TCAGTTCCTG AGGAATCCAG AATGTGCTCT CCAGCGGCTG 
ATACTGAATC ACTGCAACAT TGTAGATGAT GCTTATGGCT TCCTGGCAAT GAGACTTGCA 
AACAACACAA AGCTGACCCA CCTGAGCCTG ACCATGAACC CCGTAGGGGA TGGTGCAATG 
AAGCTACTGT GTGAAGCTTT AAAGGAACCT ACTTGTTACC TTCAAGAACT GGAACTAGTG 
GACTGCCAAC TCACACAGAA CTGCTGCGAG GACCTGGCCT GTATGATCAC AACAACCAAG 
CACTTAAAAA GTTTGGATCT TGGTAACAAC GCCCTGGGTG ACAAAGGAGT CATAACCCTG 
TGTGAGGGAC TGAAGCAAAG TAGCAGCTCC CTGAGGAGAC TTGGGTTGGG GGCATGTAAG 
TTGACTTCCA ATTGCTGTGA GGCATTGTCA TTGGCCATCT CTTGCAACCC TCACCTGAAC 
AGCCTAAACC TGGTGAAGAA TGACTTCAGT ACATCGGGGA TGTTGAAGCT GTGCTCTGCG 
TTCCAATGCC CTGTCTCTAA CCTGGGGATA ATTGGCCTGT GGAAGCAGGA GTACTATGCC 
CGAGTGAGAA GACAGCTGGA GGAAGTTGAG TTTGTCAAGC CCCACGTGGT GATTGATGGT 
GATTGGTATG CTAGTGATGA AGATGACCGA AACTGGTGGA AAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034232.1
CDS	76..3363
Translation	MGPPEKESKAILKARGLEEEQKSERKMTSPENDSKSIQKDQGPEQEQTSESTMGPPEKDSKAILKARGLEEEQKSESTMSPSENVSRAILKDSGSEEVEQASERKMTSPENDSKSIQKDQGPEQEQTSDNGGDLQDYKAHVIAKFDTSVDLHYDSPEMKLLSDAFKPYQKTFQPHTIILHGRPGVGKSALARSIVLGWAQGKLFQKMSFVIFFSVREIKWTEKSSLAQLIAKECPDSWDLVTKIMSQPERLLFVIDGLDDMDSVLQHDDMTLSRDWKDEQPIYILMYSLLRKALLPQSFLIITTRNTGLEKLKSMVVSPLYILVEGLSASRRSQLVLENISNESDRIQVFHSLIENHQLFDQCQAPSVCSLVCEALQLQKKLGKRCTLPCQTLTGLYATLVFHQLTLKRPSQSALSQEEQITLVGLCMMAAEGVWTMRSVFYDDDLKNYSLKESEILALFHMNILLQVGHNSEQCYVFSHLSLQDFFAALYYVLEGLEEWNQHFCFIENQRSIMEVKRTDDTRLLGMKRFLFGLMNKDILKTLEVLFEYPVIPTVEQKLQHWVSLIAQQVNGTSPMDTLDAFYCLFESQDEEFVGGALKRFQEVWLLINQKMDLKVSSYCLKHCQNLKAIRVDIRDLLSVDNTLELCPVVTVQETQCKPLLMEWWGNFCSVLGSLRNLKELDLGDSILSQRAMKILCLELRNQSCRIQKLTFKSAEVVSGLKHLWKLLFSNQNLKYLNLGNTPMKDDDMKLACEALKHPKCSVETLRLDSCELTIIGYEMISTLLISTTRLKCLSLAKNRVGVKSMISLGNALSSSMCLLQKLILDNCGLTPASCHLLVSALFSNQNLTHLCLSNNSLGTEGVQQLCQFLRNPECALQRLILNHCNIVDDAYGFLAMRLANNTKLTHLSLTMNPVGDGAMKLLCEALKEPTCYLQELELVDCQLTQNCCEDLACMITTTKHLKSLDLGNNALGDKGVITLCEGLKQSSSSLRRLGLGACKLTSNCCEALSLAISCNPHLNSLNLVKNDFSTSGMLKLCSAFQCPVSNLGIIGLWKQEYYARVRRQLEEVEFVKPHVVIDGDWYASDEDDRNWWKN

Target ORF information:

RefSeq Version	NM_001039143.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 5 (Nlrp5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039143.2

ORF Insert Sequence:

ATGGGTCCTC CAGAAAAAGA AAGTAAAGCA ATCTTGAAAG CACGTGGATT GGAAGAGGAA 
CAGAAGTCAG AAAGAAAAAT GACTTCTCCA GAAAACGACA GTAAATCAAT CCAGAAAGAC 
CAAGGACCAG AGCAGGAGCA GACATCAGAA AGCACAATGG GTCCTCCAGA AAAAGACAGT 
AAAGCAATCT TGAAAGCACG TGGATTGGAA GAGGAACAGA AGTCAGAAAG CACAATGTCT 
CCTTCAGAAA ATGTCAGTAG AGCAATCCTG AAAGACAGTG GATCAGAAGA AGTGGAACAG 
GCGTCAGAAA GAAAAATGAC TTCTCCAGAA AACGACAGTA AATCAATCCA GAAAGACCAA 
GGACCAGAGC AGGAGCAGAC ATCAGATAAT GGAGGTGACT TACAAGACTA CAAGGCCCAT 
GTGATTGCTA AGTTCGACAC AAGTGTGGAT CTACACTATG ACAGCCCAGA GATGAAATTA 
TTGTCTGATG CTTTTAAACC ATACCAGAAA ACCTTCCAGC CTCACACCAT TATCCTACAT 
GGAAGACCAG GAGTTGGGAA GTCAGCTTTG GCCAGAAGTA TTGTTCTTGG CTGGGCACAG 
GGTAAACTCT TCCAAAAAAT GTCCTTTGTC ATCTTCTTCT CTGTTAGAGA AATAAAGTGG 
ACAGAGAAGA GCAGTTTGGC ACAGCTGATT GCTAAGGAGT GTCCAGACTC CTGGGATCTA 
GTGACAAAGA TCATGTCCCA ACCAGAAAGA CTCTTGTTTG TCATAGATGG CTTGGATGAT 
ATGGACTCTG TCCTCCAACA TGATGATATG ACACTATCCA GAGACTGGAA GGATGAACAG 
CCCATATACA TCCTGATGTA CAGCCTCCTG AGGAAGGCTC TCCTACCTCA GTCCTTTCTC 
ATCATTACCA CCAGAAACAC AGGCTTAGAA AAACTCAAGT CAATGGTTGT GTCCCCCCTC 
TATATACTGG TTGAAGGACT GTCTGCATCA AGGAGATCTC AGCTGGTCCT CGAGAACATC 
TCCAATGAGT CTGATAGAAT ACAAGTCTTC CATTCTCTGA TAGAAAATCA CCAGCTGTTT 
GACCAATGCC AGGCCCCCTC TGTGTGCTCC CTGGTCTGTG AGGCTCTACA GCTACAGAAG 
AAACTGGGAA AGAGATGCAC CCTACCCTGC CAGACTCTCA CCGGTTTGTA TGCCACGTTG 
GTGTTTCACC AGCTCACCTT GAAAAGGCCT TCCCAGAGCG CTCTCAGTCA GGAAGAACAG 
ATTACTCTAG TGGGTTTGTG CATGATGGCA GCTGAAGGAG TGTGGACCAT GAGGTCGGTG 
TTCTATGATG ATGACCTGAA GAACTATAGC CTAAAGGAGT CTGAGATCTT GGCCCTCTTT 
CACATGAACA TCCTTCTCCA GGTTGGCCAC AACAGTGAGC AGTGTTATGT TTTCTCCCAC 
CTCAGCCTGC AGGATTTCTT TGCTGCCTTA TATTATGTTT TAGAAGGGCT GGAGGAATGG 
AATCAGCATT TTTGCTTCAT TGAAAACCAA AGGAGCATCA TGGAGGTGAA GAGAACTGAC 
GACACTCGCC TCCTCGGGAT GAAGCGTTTC TTATTTGGCC TCATGAACAA GGATATCTTG 
AAGACTCTGG AGGTTCTGTT TGAATATCCC GTGATTCCAA CTGTTGAGCA GAAGCTCCAA 
CACTGGGTCT CTCTGATAGC TCAGCAGGTC AATGGCACCA GCCCAATGGA CACCCTGGAT 
GCCTTCTATT GTCTATTTGA GTCTCAGGAT GAAGAGTTTG TTGGCGGGGC TCTCAAACGC 
TTCCAAGAAG TGTGGCTGCT GATTAACCAG AAGATGGACT TGAAGGTCTC TTCCTACTGT 
CTCAAGCACT GTCAGAACTT GAAGGCAATC CGGGTGGATA TCAGAGACCT CCTCTCGGTA 
GATAATACTC TCGAGCTGTG CCCTGTTGTT ACTGTCCAGG AGACACAATG TAAGCCCCTC 
CTCATGGAGT GGTGGGGAAA CTTCTGCTCT GTGCTTGGCA GCCTCCGGAA CTTGAAGGAG 
CTGGACTTGG GCGACAGCAT CCTGAGTCAA CGGGCCATGA AGATACTGTG CCTCGAGCTG 
CGGAATCAGT CCTGCAGAAT ACAGAAGCTG ACGTTTAAGA GTGCAGAGGT AGTGTCTGGC 
CTGAAACATC TCTGGAAGCT CCTTTTTAGC AATCAAAACT TAAAGTACCT CAATCTAGGG 
AACACTCCCA TGAAGGATGA TGACATGAAG TTAGCCTGCG AAGCGCTGAA ACATCCAAAG 
TGCTCCGTGG AGACTCTGAG GTTGGATTCC TGTGAGTTAA CCATCATTGG TTATGAGATG 
ATCTCCACGC TTCTTATTTC AACCACCAGG CTAAAGTGTC TCAGCCTGGC CAAAAATAGA 
GTGGGAGTAA AAAGCATGAT ATCCCTTGGG AATGCCTTGA GTAGCTCAAT GTGTCTACTG 
CAAAAGTTGA TACTGGACAA CTGTGGCCTC ACACCTGCCA GCTGCCACCT TCTGGTCTCA 
GCCCTTTTCA GCAACCAGAA CTTGACACAC CTGTGCCTGT CAAACAACAG CCTGGGGACT 
GAAGGAGTGC AACAGCTGTG TCAGTTCCTG AGGAATCCAG AATGTGCTCT CCAGCGGCTG 
ATACTGAATC ACTGCAACAT TGTAGATGAT GCTTATGGCT TCCTGGCAAT GAGACTTGCA 
AACAACACAA AGCTGACCCA CCTGAGCCTG ACCATGAACC CCGTAGGGGA TGGTGCAATG 
AAGCTACTGT GTGAAGCTTT AAAGGAACCT ACTTGTTACC TTCAAGAACT GGAACTAGTG 
GACTGCCAAC TCACACAGAA CTGCTGCGAG GACCTGGCCT GTATGATCAC AACAACCAAG 
CACTTAAAAA GTTTGGATCT TGGTAACAAC GCCCTGGGTG ACAAAGGAGT CATAACCCTG 
TGTGAGGGAC TGAAGCAAAG TAGCAGCTCC CTGAGGAGAC TTGGGTTGGG GGCATGTAAG 
TTGACTTCCA ATTGCTGTGA GGCATTGTCA TTGGCCATCT CTTGCAACCC TCACCTGAAC 
AGCCTAAACC TGGTGAAGAA TGACTTCAGT ACATCGGGGA TGTTGAAGCT GTGCTCTGCG 
TTCCAATGCC CTGTCTCTAA CCTGGGGATA ATTGGCCTGT GGAAGCAGGA GTACTATGCC 
CGAGTGAGAA GACAGCTGGA GGAAGTTGAG TTTGTCAAGC CCCACGTGGT GATTGATGGT 
GATTGGTATG CTAGTGATGA AGATGACCGA AACTGGTGGA AAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp5 ( NM_011860.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035990.1 Mus musculus NLR family, pyrin domain containing 5 (Nlrp5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16213
Clone ID Related Accession (Same CDS sequence)	NM_011860.3
Accession Version	NM_011860.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK087774.1, BC053384.1 and AC159288.4. On Apr 4, 2015 this sequence version replaced NM_011860.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053384.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849376 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCCTC CAGAAAAAGA AAGTAAAGCA ATCTTGAAAG CACGTGGATT GGAAGAGGAA 
CAGAAGTCAG AAAGAAAAAT GACTTCTCCA GAAAACGACA GTAAATCAAT CCAGAAAGAC 
CAAGGACCAG AGCAGGAGCA GACATCAGAA AGCACAATGG GTCCTCCAGA AAAAGACAGT 
AAAGCAATCT TGAAAGCACG TGGATTGGAA GAGGAACAGA AGTCAGAAAG CACAATGTCT 
CCTTCAGAAA ATGTCAGTAG AGCAATCCTG AAAGACAGTG GATCAGAAGA AGTGGAACAG 
GCGTCAGAAA GAAAAATGAC TTCTCCAGAA AACGACAGTA AATCAATCCA GAAAGACCAA 
GGACCAGAGC AGGAGCAGAC ATCAGAAACC TTACAATCTA AGGAAGAAGA TGAAGTGACA 
GAGGCAGATA AAGATAATGG AGGTGACTTA CAAGACTACA AGGCCCATGT GATTGCTAAG 
TTCGACACAA GTGTGGATCT ACACTATGAC AGCCCAGAGA TGAAATTATT GTCTGATGCT 
TTTAAACCAT ACCAGAAAAC CTTCCAGCCT CACACCATTA TCCTACATGG AAGACCAGGA 
GTTGGGAAGT CAGCTTTGGC CAGAAGTATT GTTCTTGGCT GGGCACAGGG TAAACTCTTC 
CAAAAAATGT CCTTTGTCAT CTTCTTCTCT GTTAGAGAAA TAAAGTGGAC AGAGAAGAGC 
AGTTTGGCAC AGCTGATTGC TAAGGAGTGT CCAGACTCCT GGGATCTAGT GACAAAGATC 
ATGTCCCAAC CAGAAAGACT CTTGTTTGTC ATAGATGGCT TGGATGATAT GGACTCTGTC 
CTCCAACATG ATGATATGAC ACTATCCAGA GACTGGAAGG ATGAACAGCC CATATACATC 
CTGATGTACA GCCTCCTGAG GAAGGCTCTC CTACCTCAGT CCTTTCTCAT CATTACCACC 
AGAAACACAG GCTTAGAAAA ACTCAAGTCA ATGGTTGTGT CCCCCCTCTA TATACTGGTT 
GAAGGACTGT CTGCATCAAG GAGATCTCAG CTGGTCCTCG AGAACATCTC CAATGAGTCT 
GATAGAATAC AAGTCTTCCA TTCTCTGATA GAAAATCACC AGCTGTTTGA CCAATGCCAG 
GCCCCCTCTG TGTGCTCCCT GGTCTGTGAG GCTCTACAGC TACAGAAGAA ACTGGGAAAG 
AGATGCACCC TACCCTGCCA GACTCTCACC GGTTTGTATG CCACGTTGGT GTTTCACCAG 
CTCACCTTGA AAAGGCCTTC CCAGAGCGCT CTCAGTCAGG AAGAACAGAT TACTCTAGTG 
GGTTTGTGCA TGATGGCAGC TGAAGGAGTG TGGACCATGA GGTCGGTGTT CTATGATGAT 
GACCTGAAGA ACTATAGCCT AAAGGAGTCT GAGATCTTGG CCCTCTTTCA CATGAACATC 
CTTCTCCAGG TTGGCCACAA CAGTGAGCAG TGTTATGTTT TCTCCCACCT CAGCCTGCAG 
GATTTCTTTG CTGCCTTATA TTATGTTTTA GAAGGGCTGG AGGAATGGAA TCAGCATTTT 
TGCTTCATTG AAAACCAAAG GAGCATCATG GAGGTGAAGA GAACTGACGA CACTCGCCTC 
CTCGGGATGA AGCGTTTCTT ATTTGGCCTC ATGAACAAGG ATATCTTGAA GACTCTGGAG 
GTTCTGTTTG AATATCCCGT GATTCCAACT GTTGAGCAGA AGCTCCAACA CTGGGTCTCT 
CTGATAGCTC AGCAGGTCAA TGGCACCAGC CCAATGGACA CCCTGGATGC CTTCTATTGT 
CTATTTGAGT CTCAGGATGA AGAGTTTGTT GGCGGGGCTC TCAAACGCTT CCAAGAAGTG 
TGGCTGCTGA TTAACCAGAA GATGGACTTG AAGGTCTCTT CCTACTGTCT CAAGCACTGT 
CAGAACTTGA AGGCAATCCG GGTGGATATC AGAGACCTCC TCTCGGTAGA TAATACTCTC 
GAGCTGTGCC CTGTTGTTAC TGTCCAGGAG ACACAATGTA AGCCCCTCCT CATGGAGTGG 
TGGGGAAACT TCTGCTCTGT GCTTGGCAGC CTCCGGAACT TGAAGGAGCT GGACTTGGGC 
GACAGCATCC TGAGTCAACG GGCCATGAAG ATACTGTGCC TCGAGCTGCG GAATCAGTCC 
TGCAGAATAC AGAAGCTGAC GTTTAAGAGT GCAGAGGTAG TGTCTGGCCT GAAACATCTC 
TGGAAGCTCC TTTTTAGCAA TCAAAACTTA AAGTACCTCA ATCTAGGGAA CACTCCCATG 
AAGGATGATG ACATGAAGTT AGCCTGCGAA GCGCTGAAAC ATCCAAAGTG CTCCGTGGAG 
ACTCTGAGGT TGGATTCCTG TGAGTTAACC ATCATTGGTT ATGAGATGAT CTCCACGCTT 
CTTATTTCAA CCACCAGGCT AAAGTGTCTC AGCCTGGCCA AAAATAGAGT GGGAGTAAAA 
AGCATGATAT CCCTTGGGAA TGCCTTGAGT AGCTCAATGT GTCTACTGCA AAAGTTGATA 
CTGGACAACT GTGGCCTCAC ACCTGCCAGC TGCCACCTTC TGGTCTCAGC CCTTTTCAGC 
AACCAGAACT TGACACACCT GTGCCTGTCA AACAACAGCC TGGGGACTGA AGGAGTGCAA 
CAGCTGTGTC AGTTCCTGAG GAATCCAGAA TGTGCTCTCC AGCGGCTGAT ACTGAATCAC 
TGCAACATTG TAGATGATGC TTATGGCTTC CTGGCAATGA GACTTGCAAA CAACACAAAG 
CTGACCCACC TGAGCCTGAC CATGAACCCC GTAGGGGATG GTGCAATGAA GCTACTGTGT 
GAAGCTTTAA AGGAACCTAC TTGTTACCTT CAAGAACTGG AACTAGTGGA CTGCCAACTC 
ACACAGAACT GCTGCGAGGA CCTGGCCTGT ATGATCACAA CAACCAAGCA CTTAAAAAGT 
TTGGATCTTG GTAACAACGC CCTGGGTGAC AAAGGAGTCA TAACCCTGTG TGAGGGACTG 
AAGCAAAGTA GCAGCTCCCT GAGGAGACTT GGGTTGGGGG CATGTAAGTT GACTTCCAAT 
TGCTGTGAGG CATTGTCATT GGCCATCTCT TGCAACCCTC ACCTGAACAG CCTAAACCTG 
GTGAAGAATG ACTTCAGTAC ATCGGGGATG TTGAAGCTGT GCTCTGCGTT CCAATGCCCT 
GTCTCTAACC TGGGGATAAT TGGCCTGTGG AAGCAGGAGT ACTATGCCCG AGTGAGAAGA 
CAGCTGGAGG AAGTTGAGTT TGTCAAGCCC CACGTGGTGA TTGATGGTGA TTGGTATGCT 
AGTGATGAAG ATGACCGAAA CTGGTGGAAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035990.1
CDS	76..3411
Translation	MGPPEKESKAILKARGLEEEQKSERKMTSPENDSKSIQKDQGPEQEQTSESTMGPPEKDSKAILKARGLEEEQKSESTMSPSENVSRAILKDSGSEEVEQASERKMTSPENDSKSIQKDQGPEQEQTSETLQSKEEDEVTEADKDNGGDLQDYKAHVIAKFDTSVDLHYDSPEMKLLSDAFKPYQKTFQPHTIILHGRPGVGKSALARSIVLGWAQGKLFQKMSFVIFFSVREIKWTEKSSLAQLIAKECPDSWDLVTKIMSQPERLLFVIDGLDDMDSVLQHDDMTLSRDWKDEQPIYILMYSLLRKALLPQSFLIITTRNTGLEKLKSMVVSPLYILVEGLSASRRSQLVLENISNESDRIQVFHSLIENHQLFDQCQAPSVCSLVCEALQLQKKLGKRCTLPCQTLTGLYATLVFHQLTLKRPSQSALSQEEQITLVGLCMMAAEGVWTMRSVFYDDDLKNYSLKESEILALFHMNILLQVGHNSEQCYVFSHLSLQDFFAALYYVLEGLEEWNQHFCFIENQRSIMEVKRTDDTRLLGMKRFLFGLMNKDILKTLEVLFEYPVIPTVEQKLQHWVSLIAQQVNGTSPMDTLDAFYCLFESQDEEFVGGALKRFQEVWLLINQKMDLKVSSYCLKHCQNLKAIRVDIRDLLSVDNTLELCPVVTVQETQCKPLLMEWWGNFCSVLGSLRNLKELDLGDSILSQRAMKILCLELRNQSCRIQKLTFKSAEVVSGLKHLWKLLFSNQNLKYLNLGNTPMKDDDMKLACEALKHPKCSVETLRLDSCELTIIGYEMISTLLISTTRLKCLSLAKNRVGVKSMISLGNALSSSMCLLQKLILDNCGLTPASCHLLVSALFSNQNLTHLCLSNNSLGTEGVQQLCQFLRNPECALQRLILNHCNIVDDAYGFLAMRLANNTKLTHLSLTMNPVGDGAMKLLCEALKEPTCYLQELELVDCQLTQNCCEDLACMITTTKHLKSLDLGNNALGDKGVITLCEGLKQSSSSLRRLGLGACKLTSNCCEALSLAISCNPHLNSLNLVKNDFSTSGMLKLCSAFQCPVSNLGIIGLWKQEYYARVRRQLEEVEFVKPHVVIDGDWYASDEDDRNWWKN

Target ORF information:

RefSeq Version	NM_011860.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 5 (Nlrp5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011860.3

ORF Insert Sequence:

ATGGGTCCTC CAGAAAAAGA AAGTAAAGCA ATCTTGAAAG CACGTGGATT GGAAGAGGAA 
CAGAAGTCAG AAAGAAAAAT GACTTCTCCA GAAAACGACA GTAAATCAAT CCAGAAAGAC 
CAAGGACCAG AGCAGGAGCA GACATCAGAA AGCACAATGG GTCCTCCAGA AAAAGACAGT 
AAAGCAATCT TGAAAGCACG TGGATTGGAA GAGGAACAGA AGTCAGAAAG CACAATGTCT 
CCTTCAGAAA ATGTCAGTAG AGCAATCCTG AAAGACAGTG GATCAGAAGA AGTGGAACAG 
GCGTCAGAAA GAAAAATGAC TTCTCCAGAA AACGACAGTA AATCAATCCA GAAAGACCAA 
GGACCAGAGC AGGAGCAGAC ATCAGAAACC TTACAATCTA AGGAAGAAGA TGAAGTGACA 
GAGGCAGATA AAGATAATGG AGGTGACTTA CAAGACTACA AGGCCCATGT GATTGCTAAG 
TTCGACACAA GTGTGGATCT ACACTATGAC AGCCCAGAGA TGAAATTATT GTCTGATGCT 
TTTAAACCAT ACCAGAAAAC CTTCCAGCCT CACACCATTA TCCTACATGG AAGACCAGGA 
GTTGGGAAGT CAGCTTTGGC CAGAAGTATT GTTCTTGGCT GGGCACAGGG TAAACTCTTC 
CAAAAAATGT CCTTTGTCAT CTTCTTCTCT GTTAGAGAAA TAAAGTGGAC AGAGAAGAGC 
AGTTTGGCAC AGCTGATTGC TAAGGAGTGT CCAGACTCCT GGGATCTAGT GACAAAGATC 
ATGTCCCAAC CAGAAAGACT CTTGTTTGTC ATAGATGGCT TGGATGATAT GGACTCTGTC 
CTCCAACATG ATGATATGAC ACTATCCAGA GACTGGAAGG ATGAACAGCC CATATACATC 
CTGATGTACA GCCTCCTGAG GAAGGCTCTC CTACCTCAGT CCTTTCTCAT CATTACCACC 
AGAAACACAG GCTTAGAAAA ACTCAAGTCA ATGGTTGTGT CCCCCCTCTA TATACTGGTT 
GAAGGACTGT CTGCATCAAG GAGATCTCAG CTGGTCCTCG AGAACATCTC CAATGAGTCT 
GATAGAATAC AAGTCTTCCA TTCTCTGATA GAAAATCACC AGCTGTTTGA CCAATGCCAG 
GCCCCCTCTG TGTGCTCCCT GGTCTGTGAG GCTCTACAGC TACAGAAGAA ACTGGGAAAG 
AGATGCACCC TACCCTGCCA GACTCTCACC GGTTTGTATG CCACGTTGGT GTTTCACCAG 
CTCACCTTGA AAAGGCCTTC CCAGAGCGCT CTCAGTCAGG AAGAACAGAT TACTCTAGTG 
GGTTTGTGCA TGATGGCAGC TGAAGGAGTG TGGACCATGA GGTCGGTGTT CTATGATGAT 
GACCTGAAGA ACTATAGCCT AAAGGAGTCT GAGATCTTGG CCCTCTTTCA CATGAACATC 
CTTCTCCAGG TTGGCCACAA CAGTGAGCAG TGTTATGTTT TCTCCCACCT CAGCCTGCAG 
GATTTCTTTG CTGCCTTATA TTATGTTTTA GAAGGGCTGG AGGAATGGAA TCAGCATTTT 
TGCTTCATTG AAAACCAAAG GAGCATCATG GAGGTGAAGA GAACTGACGA CACTCGCCTC 
CTCGGGATGA AGCGTTTCTT ATTTGGCCTC ATGAACAAGG ATATCTTGAA GACTCTGGAG 
GTTCTGTTTG AATATCCCGT GATTCCAACT GTTGAGCAGA AGCTCCAACA CTGGGTCTCT 
CTGATAGCTC AGCAGGTCAA TGGCACCAGC CCAATGGACA CCCTGGATGC CTTCTATTGT 
CTATTTGAGT CTCAGGATGA AGAGTTTGTT GGCGGGGCTC TCAAACGCTT CCAAGAAGTG 
TGGCTGCTGA TTAACCAGAA GATGGACTTG AAGGTCTCTT CCTACTGTCT CAAGCACTGT 
CAGAACTTGA AGGCAATCCG GGTGGATATC AGAGACCTCC TCTCGGTAGA TAATACTCTC 
GAGCTGTGCC CTGTTGTTAC TGTCCAGGAG ACACAATGTA AGCCCCTCCT CATGGAGTGG 
TGGGGAAACT TCTGCTCTGT GCTTGGCAGC CTCCGGAACT TGAAGGAGCT GGACTTGGGC 
GACAGCATCC TGAGTCAACG GGCCATGAAG ATACTGTGCC TCGAGCTGCG GAATCAGTCC 
TGCAGAATAC AGAAGCTGAC GTTTAAGAGT GCAGAGGTAG TGTCTGGCCT GAAACATCTC 
TGGAAGCTCC TTTTTAGCAA TCAAAACTTA AAGTACCTCA ATCTAGGGAA CACTCCCATG 
AAGGATGATG ACATGAAGTT AGCCTGCGAA GCGCTGAAAC ATCCAAAGTG CTCCGTGGAG 
ACTCTGAGGT TGGATTCCTG TGAGTTAACC ATCATTGGTT ATGAGATGAT CTCCACGCTT 
CTTATTTCAA CCACCAGGCT AAAGTGTCTC AGCCTGGCCA AAAATAGAGT GGGAGTAAAA 
AGCATGATAT CCCTTGGGAA TGCCTTGAGT AGCTCAATGT GTCTACTGCA AAAGTTGATA 
CTGGACAACT GTGGCCTCAC ACCTGCCAGC TGCCACCTTC TGGTCTCAGC CCTTTTCAGC 
AACCAGAACT TGACACACCT GTGCCTGTCA AACAACAGCC TGGGGACTGA AGGAGTGCAA 
CAGCTGTGTC AGTTCCTGAG GAATCCAGAA TGTGCTCTCC AGCGGCTGAT ACTGAATCAC 
TGCAACATTG TAGATGATGC TTATGGCTTC CTGGCAATGA GACTTGCAAA CAACACAAAG 
CTGACCCACC TGAGCCTGAC CATGAACCCC GTAGGGGATG GTGCAATGAA GCTACTGTGT 
GAAGCTTTAA AGGAACCTAC TTGTTACCTT CAAGAACTGG AACTAGTGGA CTGCCAACTC 
ACACAGAACT GCTGCGAGGA CCTGGCCTGT ATGATCACAA CAACCAAGCA CTTAAAAAGT 
TTGGATCTTG GTAACAACGC CCTGGGTGAC AAAGGAGTCA TAACCCTGTG TGAGGGACTG 
AAGCAAAGTA GCAGCTCCCT GAGGAGACTT GGGTTGGGGG CATGTAAGTT GACTTCCAAT 
TGCTGTGAGG CATTGTCATT GGCCATCTCT TGCAACCCTC ACCTGAACAG CCTAAACCTG 
GTGAAGAATG ACTTCAGTAC ATCGGGGATG TTGAAGCTGT GCTCTGCGTT CCAATGCCCT 
GTCTCTAACC TGGGGATAAT TGGCCTGTGG AAGCAGGAGT ACTATGCCCG AGTGAGAAGA 
CAGCTGGAGG AAGTTGAGTT TGTCAAGCCC CACGTGGTGA TTGATGGTGA TTGGTATGCT 
AGTGATGAAG ATGACCGAAA CTGGTGGAAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp5 ( NM_001305857.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292786.1 Mus musculus NLR family, pyrin domain containing 5 (Nlrp5), transcript variant 3, mRNA.

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CloneID	OMu69157
Clone ID Related Accession (Same CDS sequence)	NM_001305857.1
Accession Version	NM_001305857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Mus musculus(house mouse)
Product	NACHT, LRR and PYD domains-containing protein 5 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK087774.1, BC053384.1 and AC159288.4. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform c, which is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY329489.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849382 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCCTC CAGAAAAAGA AAGTAAAGCA ATCTTGAAAG CACGTGGATT GGAAGAGGAA 
CAGAAGTCAG AAAGAAAAAT GACTTCTCCA GAAAACGACA GTAAATCAAT CCAGAAAGAC 
CAAGGACCAG AGCAGGAGCA GACATCAGAA AGCACAATGG GTCCTCCAGA AAAAGACAGT 
AAAGCAATCT TGAAAGCACG TGGATTGGAA GAGGAACAGA AGTCAGAAAG CACAATGTCT 
CCTTCAGAAA ATGTCAGTAG AGCAATCCTG AAAGACAGTG GATCAGAAGA AGTGGAACAG 
GCGTCAGAAA GAAAAATGAC TTCTCCAGAA AACGACAGTA AATCAATCCA GAAAGACCAA 
GGACCAGAGC AGGAGCAGAC ATCAGAAACC TTACAATCTA AGGAAGAAGA TGAAGTGACA 
GAGGCAGATA AAGATAATGG AGGTGACTTA CAAGACTACA AGGCCCATGT GATTGCTAAG 
TTCGACACAA GTGTGGATCT ACACTATGAC AGCCCAGAGA TGAAATTATT GTCTGATGCT 
TTTAAACCAT ACCAGAAAAC CTTCCAGCCT CACACCATTA TCCTACATGG AAGACCAGGA 
GTTGGGAAGT CAGCTTTGGC CAGAAGTATT GTTCTTGGCT GGGCACAGGG TAAACTCTTC 
CAAAAAATGT CCTTTGTCAT CTTCTTCTCT GTTAGAGAAA TAAAGTGGAC AGAGAAGAGC 
AGTTTGGCAC AGCTGATTGC TAAGGAGTGT CCAGACTCCT GGGATCTAGT GACAAAGATC 
ATGTCCCAAC CAGAAAGACT CTTGTTTGTC ATAGATGGCT TGGATGATAT GGACTCTGTC 
CTCCAACATG ATGATATGAC ACTATCCAGA GACTGGAAGG ATGAACAGCC CATATACATC 
CTGATGTACA GCCTCCTGAG GAAGGCTCTC CTACCTCAGT CCTTTCTCAT CATTACCACC 
AGAAACACAG GCTTAGAAAA ACTCAAGTCA ATGGTTGTGT CCCCCCTCTA TATACTGGTT 
GAAGGACTGT CTGCATCAAG GAGATCTCAG CTGGTCCTCG AGAACATCTC CAATGAGTCT 
GATAGAATAC AAGTCTTCCA TTCTCTGATA GAAAATCACC AGCTGTTTGA CCAATGCCAG 
GCCCCCTCTG TGTGCTCCCT GGTCTGTGAG GCTCTACAGC TACAGAAGAA ACTGGGAAAG 
AGATGCACCC TACCCTGCCA GACTCTCACC GGTTTGTATG CCACGTTGGT GTTTCACCAG 
CTCACCTTGA AAAGGCCTTC CCAGAGCGCT CTCAGTCAGG AAGAACAGAT TACTCTAGTG 
GGTTTGTGCA TGATGGCAGC TGAAGGAGTG TGGACCATGA GGTCGGTGTT CTATGATGAT 
GACCTGAAGA ACTATAGCCT AAAGGAGTCT GAGATCTTGG CCCTCTTTCA CATGAACATC 
CTTCTCCAGG TTGGCCACAA CAGTGAGCAG TGTTATGTTT TCTCCCACCT CAGCCTGCAG 
GATTTCTTTG CTGCCTTATA TTATGTTTTA GAAGGGCTGG AGGAATGGAA TCAGCATTTT 
TGCTTCATTG AAAACCAAAG GAGCATCATG GAGGTGAAGA GAACTGACGA CACTCGCCTC 
CTCGGGATGA AGCGTTTCTT ATTTGGCCTC ATGAACAAGG ATATCTTGAA GACTCTGGAG 
GTTCTGTTTG AATATCCCGT GATTCCAACT GTTGAGCAGA AGCTCCAACA CTGGGTCTCT 
CTGATAGCTC AGCAGGTCAA TGGCACCAGC CCAATGGACA CCCTGGATGC CTTCTATTGT 
CTATTTGAGT CTCAGGATGA AGAGTTTGTT GGCGGGGCTC TCAAACGCTT CCAAGAAGTG 
TGGCTGCTGA TTAACCAGAA GATGGACTTG AAGGTCTCTT CCTACTGTCT CAAGCACTGT 
CAGAACTTGA AGGCAATCCG GGTGGATATC AGAGACCTCC TCTCGGTAGA TAATACTCTC 
GAGCTGTGCC CTGTTGTTAC TGTCCAGGAG ACACAATGTA AGCCCCTCCT CATGGAGTGG 
TGGGGAAACT TCTGCTCTGT GCTTGGCAGC CTCCGGAACT TGAAGGAGCT GGACTTGGGC 
GACAGCATCC TGAGTCAACG GGCCATGAAG ATACTGTGCC TCGAGCTGCG GAATCAGTCC 
TGCAGAATAC AGAAGCTGAC GTTTAAGAGT GCAGAGGTAG TGTCTGGCCT GAAACATCTC 
TGGAAGCTCC TTTTTAGCAA TCAAAACTTA AAGTACCTCA ATCTAGGGAA CACTCCCATG 
AAGGATGATG ACATGAAGTT AGCCTGCGAA GCGCTGAAAC ATCCAAAGTG CTCCGTGGAG 
ACTCTGAGGT TGGATTCCTG TGAGTTAACC ATCATTGGTT ATGAGATGAT CTCCACGCTT 
CTTATTTCAA CCACCAGGCT AAAGTGTCTC AGCCTGGCCA AAAATAGAGT GGGAGTAAAA 
AGCATGATAT CCCTTGGGAA TGCCTTGAGT AGCTCAATGT GTCTACTGCA AAAGTTGATA 
CTGGACAACT GTGGCCTCAC ACCTGCCAGC TGCCACCTTC TGGTCTCAGC CCTTTTCAGC 
AACCAGAACT TGACACACCT GTGCCTGTCA AACAACAGCC TGGGGACTGA AGGAGTGCAA 
CAGCTGTGTC AGTTCCTGAG GAATCCAGAA TGTGCTCTCC AGCGGCTGAT ACTGAATCAC 
TGCAACATTG TAGATGATGC TTATGGCTTC CTGGCAATGA GACTTGCAAA CAACACAAAG 
CTGACCCACC TGAGCCTGAC CATGAACCCC GTAGGGGATG GTGCAATGAA GCTACTGTGT 
GAAGCTTTAA AGGAACCTAC TTGTTACCTT CAAGAACTGG AACTAGTGGA CTGCCAACTC 
ACACAGAACT GCTGCGAGGA CCTGGCCTGT ATGATCACAA CAACCAAGCA CTTAAAAAGT 
TTGGATCTTG GTAACAACGC CCTGGGTGAC AAAGGAGTCA TAACCCTGTG TGAGGGACTG 
AAGCAAAGTA GCAGCTCCCT GAGGAGACTT GGCCTAAACC TGGTGAAGAA TGACTTCAGT 
ACATCGGGGA TGTTGAAGCT GTGCTCTGCG TTCCAATGCC CTGTCTCTAA CCTGGGGATA 
ATTGGCCTGT GGAAGCAGGA GTACTATGCC CGAGTGAGAA GACAGCTGGA GGAAGTTGAG 
TTTGTCAAGC CCCACGTGGT GATTGATGGT GATTGGTATG CTAGTGATGA AGATGACCGA 
AACTGGTGGA AAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292786.1
CDS	9..3266
Translation	MGPPEKESKAILKARGLEEEQKSERKMTSPENDSKSIQKDQGPEQEQTSESTMGPPEKDSKAILKARGLEEEQKSESTMSPSENVSRAILKDSGSEEVEQASERKMTSPENDSKSIQKDQGPEQEQTSETLQSKEEDEVTEADKDNGGDLQDYKAHVIAKFDTSVDLHYDSPEMKLLSDAFKPYQKTFQPHTIILHGRPGVGKSALARSIVLGWAQGKLFQKMSFVIFFSVREIKWTEKSSLAQLIAKECPDSWDLVTKIMSQPERLLFVIDGLDDMDSVLQHDDMTLSRDWKDEQPIYILMYSLLRKALLPQSFLIITTRNTGLEKLKSMVVSPLYILVEGLSASRRSQLVLENISNESDRIQVFHSLIENHQLFDQCQAPSVCSLVCEALQLQKKLGKRCTLPCQTLTGLYATLVFHQLTLKRPSQSALSQEEQITLVGLCMMAAEGVWTMRSVFYDDDLKNYSLKESEILALFHMNILLQVGHNSEQCYVFSHLSLQDFFAALYYVLEGLEEWNQHFCFIENQRSIMEVKRTDDTRLLGMKRFLFGLMNKDILKTLEVLFEYPVIPTVEQKLQHWVSLIAQQVNGTSPMDTLDAFYCLFESQDEEFVGGALKRFQEVWLLINQKMDLKVSSYCLKHCQNLKAIRVDIRDLLSVDNTLELCPVVTVQETQCKPLLMEWWGNFCSVLGSLRNLKELDLGDSILSQRAMKILCLELRNQSCRIQKLTFKSAEVVSGLKHLWKLLFSNQNLKYLNLGNTPMKDDDMKLACEALKHPKCSVETLRLDSCELTIIGYEMISTLLISTTRLKCLSLAKNRVGVKSMISLGNALSSSMCLLQKLILDNCGLTPASCHLLVSALFSNQNLTHLCLSNNSLGTEGVQQLCQFLRNPECALQRLILNHCNIVDDAYGFLAMRLANNTKLTHLSLTMNPVGDGAMKLLCEALKEPTCYLQELELVDCQLTQNCCEDLACMITTTKHLKSLDLGNNALGDKGVITLCEGLKQSSSSLRRLGLNLVKNDFSTSGMLKLCSAFQCPVSNLGIIGLWKQEYYARVRRQLEEVEFVKPHVVIDGDWYASDEDDRNWWKN

Target ORF information:

RefSeq Version	NM_001305857.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NLR family, pyrin domain containing 5 (Nlrp5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305857.1

ORF Insert Sequence:

ATGGGTCCTC CAGAAAAAGA AAGTAAAGCA ATCTTGAAAG CACGTGGATT GGAAGAGGAA 
CAGAAGTCAG AAAGAAAAAT GACTTCTCCA GAAAACGACA GTAAATCAAT CCAGAAAGAC 
CAAGGACCAG AGCAGGAGCA GACATCAGAA AGCACAATGG GTCCTCCAGA AAAAGACAGT 
AAAGCAATCT TGAAAGCACG TGGATTGGAA GAGGAACAGA AGTCAGAAAG CACAATGTCT 
CCTTCAGAAA ATGTCAGTAG AGCAATCCTG AAAGACAGTG GATCAGAAGA AGTGGAACAG 
GCGTCAGAAA GAAAAATGAC TTCTCCAGAA AACGACAGTA AATCAATCCA GAAAGACCAA 
GGACCAGAGC AGGAGCAGAC ATCAGAAACC TTACAATCTA AGGAAGAAGA TGAAGTGACA 
GAGGCAGATA AAGATAATGG AGGTGACTTA CAAGACTACA AGGCCCATGT GATTGCTAAG 
TTCGACACAA GTGTGGATCT ACACTATGAC AGCCCAGAGA TGAAATTATT GTCTGATGCT 
TTTAAACCAT ACCAGAAAAC CTTCCAGCCT CACACCATTA TCCTACATGG AAGACCAGGA 
GTTGGGAAGT CAGCTTTGGC CAGAAGTATT GTTCTTGGCT GGGCACAGGG TAAACTCTTC 
CAAAAAATGT CCTTTGTCAT CTTCTTCTCT GTTAGAGAAA TAAAGTGGAC AGAGAAGAGC 
AGTTTGGCAC AGCTGATTGC TAAGGAGTGT CCAGACTCCT GGGATCTAGT GACAAAGATC 
ATGTCCCAAC CAGAAAGACT CTTGTTTGTC ATAGATGGCT TGGATGATAT GGACTCTGTC 
CTCCAACATG ATGATATGAC ACTATCCAGA GACTGGAAGG ATGAACAGCC CATATACATC 
CTGATGTACA GCCTCCTGAG GAAGGCTCTC CTACCTCAGT CCTTTCTCAT CATTACCACC 
AGAAACACAG GCTTAGAAAA ACTCAAGTCA ATGGTTGTGT CCCCCCTCTA TATACTGGTT 
GAAGGACTGT CTGCATCAAG GAGATCTCAG CTGGTCCTCG AGAACATCTC CAATGAGTCT 
GATAGAATAC AAGTCTTCCA TTCTCTGATA GAAAATCACC AGCTGTTTGA CCAATGCCAG 
GCCCCCTCTG TGTGCTCCCT GGTCTGTGAG GCTCTACAGC TACAGAAGAA ACTGGGAAAG 
AGATGCACCC TACCCTGCCA GACTCTCACC GGTTTGTATG CCACGTTGGT GTTTCACCAG 
CTCACCTTGA AAAGGCCTTC CCAGAGCGCT CTCAGTCAGG AAGAACAGAT TACTCTAGTG 
GGTTTGTGCA TGATGGCAGC TGAAGGAGTG TGGACCATGA GGTCGGTGTT CTATGATGAT 
GACCTGAAGA ACTATAGCCT AAAGGAGTCT GAGATCTTGG CCCTCTTTCA CATGAACATC 
CTTCTCCAGG TTGGCCACAA CAGTGAGCAG TGTTATGTTT TCTCCCACCT CAGCCTGCAG 
GATTTCTTTG CTGCCTTATA TTATGTTTTA GAAGGGCTGG AGGAATGGAA TCAGCATTTT 
TGCTTCATTG AAAACCAAAG GAGCATCATG GAGGTGAAGA GAACTGACGA CACTCGCCTC 
CTCGGGATGA AGCGTTTCTT ATTTGGCCTC ATGAACAAGG ATATCTTGAA GACTCTGGAG 
GTTCTGTTTG AATATCCCGT GATTCCAACT GTTGAGCAGA AGCTCCAACA CTGGGTCTCT 
CTGATAGCTC AGCAGGTCAA TGGCACCAGC CCAATGGACA CCCTGGATGC CTTCTATTGT 
CTATTTGAGT CTCAGGATGA AGAGTTTGTT GGCGGGGCTC TCAAACGCTT CCAAGAAGTG 
TGGCTGCTGA TTAACCAGAA GATGGACTTG AAGGTCTCTT CCTACTGTCT CAAGCACTGT 
CAGAACTTGA AGGCAATCCG GGTGGATATC AGAGACCTCC TCTCGGTAGA TAATACTCTC 
GAGCTGTGCC CTGTTGTTAC TGTCCAGGAG ACACAATGTA AGCCCCTCCT CATGGAGTGG 
TGGGGAAACT TCTGCTCTGT GCTTGGCAGC CTCCGGAACT TGAAGGAGCT GGACTTGGGC 
GACAGCATCC TGAGTCAACG GGCCATGAAG ATACTGTGCC TCGAGCTGCG GAATCAGTCC 
TGCAGAATAC AGAAGCTGAC GTTTAAGAGT GCAGAGGTAG TGTCTGGCCT GAAACATCTC 
TGGAAGCTCC TTTTTAGCAA TCAAAACTTA AAGTACCTCA ATCTAGGGAA CACTCCCATG 
AAGGATGATG ACATGAAGTT AGCCTGCGAA GCGCTGAAAC ATCCAAAGTG CTCCGTGGAG 
ACTCTGAGGT TGGATTCCTG TGAGTTAACC ATCATTGGTT ATGAGATGAT CTCCACGCTT 
CTTATTTCAA CCACCAGGCT AAAGTGTCTC AGCCTGGCCA AAAATAGAGT GGGAGTAAAA 
AGCATGATAT CCCTTGGGAA TGCCTTGAGT AGCTCAATGT GTCTACTGCA AAAGTTGATA 
CTGGACAACT GTGGCCTCAC ACCTGCCAGC TGCCACCTTC TGGTCTCAGC CCTTTTCAGC 
AACCAGAACT TGACACACCT GTGCCTGTCA AACAACAGCC TGGGGACTGA AGGAGTGCAA 
CAGCTGTGTC AGTTCCTGAG GAATCCAGAA TGTGCTCTCC AGCGGCTGAT ACTGAATCAC 
TGCAACATTG TAGATGATGC TTATGGCTTC CTGGCAATGA GACTTGCAAA CAACACAAAG 
CTGACCCACC TGAGCCTGAC CATGAACCCC GTAGGGGATG GTGCAATGAA GCTACTGTGT 
GAAGCTTTAA AGGAACCTAC TTGTTACCTT CAAGAACTGG AACTAGTGGA CTGCCAACTC 
ACACAGAACT GCTGCGAGGA CCTGGCCTGT ATGATCACAA CAACCAAGCA CTTAAAAAGT 
TTGGATCTTG GTAACAACGC CCTGGGTGAC AAAGGAGTCA TAACCCTGTG TGAGGGACTG 
AAGCAAAGTA GCAGCTCCCT GAGGAGACTT GGCCTAAACC TGGTGAAGAA TGACTTCAGT 
ACATCGGGGA TGTTGAAGCT GTGCTCTGCG TTCCAATGCC CTGTCTCTAA CCTGGGGATA 
ATTGGCCTGT GGAAGCAGGA GTACTATGCC CGAGTGAGAA GACAGCTGGA GGAAGTTGAG 
TTTGTCAAGC CCCACGTGGT GATTGATGGT GATTGGTATG CTAGTGATGA AGATGACCGA 
AACTGGTGGA AAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The role of MATER in endoplasmic reticulum distribution and calcium homeostasis in mouse oocytes.
Developmental biology386(2)331-9(2014 Feb)
Kim B,Zhang X,Kan R,Cohen R,Mukai C,Travis AJ,Coonrod SA

NLRP5 mediates mitochondrial function in mouse oocytes and embryos.
Biology of reproduction86(5)138, 1-10(2012 May)
Fernandes R,Tsuda C,Perumalsamy AL,Naranian T,Chong J,Acton BM,Tong ZB,Nelson LM,Jurisicova A

Autoimmune oophoritis with multiple molecular targets mitigated by transgenic expression of mater.
Endocrinology152(6)2465-73(2011 Jun)
Otsuka N,Tong ZB,Vanevski K,Tu W,Cheng MH,Nelson LM

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Nlrp5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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