Mylk4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mylk4
Entrez Gene ID	238564
Full Name	myosin light chain kinase family, member 4
Synonyms	EG238564
General protein information	Preferred Names myosin light chain kinase family, member 4 Names myosin light chain kinase family member 4 sgK085 sugen kinase 85
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A3.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001368880.1	NP_001355809.1	myosin light chain kinase family member 4
XM_006516667.2	XP_006516730.1	myosin light chain kinase family member 4 isoform X2
XM_006516668.3	XP_006516731.1	myosin light chain kinase family member 4 isoform X3
NM_001166030.1	NP_001159502.1	myosin light chain kinase family member 4
XM_006516666.3	XP_006516729.1	myosin light chain kinase family member 4 isoform X1
XM_006516670.3	XP_006516733.1	myosin light chain kinase family member 4 isoform X4
XM_006516667.3	XP_006516730.1	myosin light chain kinase family member 4 isoform X2
XM_006516668.4	XP_006516731.1	myosin light chain kinase family member 4 isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MYLK4	XP_005640034.1
Bos taurus (cattle)	MYLK4	XP_002697631.3
Pan troglodytes (chimpanzee)	MYLK4	XP_527218.2
	MYLK4	XP_001090801.1
Gallus gallus (chicken)	MYLK4	XP_418978.4
Xenopus tropicalis (tropical clawed frog)	LOC100497991	XP_004915362.1
Mus musculus (house mouse)	Mylk4	NP_001159502.1
Danio rerio (zebrafish)	LOC566845	XP_695224.6
Homo sapiens (human)	MYLK4	NP_001012418.2
Rattus norvegicus (Norway rat)	Mylk4	XP_006253927.1

Gene Ontology
Bibliography

Related articles in PubMed

Cardiac myosin light chain is phosphorylated by Ca2+/calmodulin-dependent and -independent kinase activities.
Chang AN, Mahajan P, Knapp S, Barton H, Sweeney HL, Kamm KE, Stull JT
Proceedings of the National Academy of Sciences of the United States of America113(27)E3824-33(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In contrast to smooth muscle MLCK and skeletal muscle MLCK, which lack activity in the absence of Ca(2+)/calmodulin, cardiac MLCK has constitutive activity that is stimulated by Ca(2+)/calmodulin.
Title: Cardiac myosin light chain is phosphorylated by Ca2+/calmodulin-dependent and -independent kinase activities.

The following Mylk4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mylk4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10291	NM_001166030.1 Latest version!	Mus musculus myosin light chain kinase family, member 4 (Mylk4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu48389	XM_006516670.3 Latest version!	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu48385	XM_006516666.3 Latest version!	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu84643	NM_001368880.1 Latest version!	Mus musculus myosin light chain kinase family, member 4 (Mylk4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu48386	XM_006516667.3 Latest version!	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu48387	XM_006516668.4 Latest version!	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu48386	XM_006516667.2	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu48387	XM_006516668.3	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mylk4 ( NM_001166030.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001159502.1 Mus musculus myosin light chain kinase family, member 4 (Mylk4), mRNA.

Price & Availability↑

CloneID	OMu10291
Clone ID Related Accession (Same CDS sequence)	NM_001166030.1
Accession Version	NM_001166030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-14
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase family member 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL645808.13. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments of mouse and human transcripts and proteins. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01164137 [ECO:0000350] ##Evidence-Data-END##

ATGTTGAAGG TGAAGAGGCT GGAAGAAATC AGCTCGTGCC ACAGCAGCAA CCCGCTGGAG 
AAGGTGGCGT TTTTCCAGTG CATGGAGGAG GTGGAGAAGG TGAAATGCTT CCTGGAGGAG 
AATTCGGGGG ATCTGGACCT GCAGTCTGGA GACAATGAGG CTGAGGAAAA TGTATGGTCC 
AACCGTGCAC TAGATGAAAG GATAATTGTC AAAGGAGGAA GGACATCAGC CCTCACAGAT 
GACATCCCTG CTCCTGCCGC TCCATTTGAT CATCGGATGG TAATGGCCAA ACATGCTTCA 
GTGGACAATC TGTACACCGT GAGCAAATCT GAGATCCTAG GAGGGGGGCG TTTTGGTCAG 
GTACATAAGT GTGAAGAGAA AGCAACAGGC CTGAAGCTGG CTGCTAAAAT CATTAAGACT 
AGAGGTGCAA AGGACAAGGA GGATGTGAAG AACGAGATCA GCGTCATGAA CCAGCTGGAC 
CACGTGAACC TCATTCAGCT CTATGATGCC TTTGAGTCCA AGCATGACAT CATCCTTGTC 
ATGGAGTATG TGGAGGGTGG GGAGCTCTTT GACCGCATCA TTGATGAAAA CTGCAACTTG 
ACAGAGCTGG ACACCATCCT GTTCATGAAG CAGATCTGTG AGGGGATACG GTACATGCAC 
CAGATGTACA TCCTCCACTT GGATCTCAAG CCTGAGAACA TCCTGTGTGT GAATCGGGAT 
GCTAAGCAAA TAAAAATTAT TGATTTTGGA TTGGCCAGAA GATACAAGCC CAGAGAGAAG 
CTGAAGGTGA ACTTTGGAAC CCCAGAATTT CTTGCCCCTG AAGTTGTGAA CTATGATTTC 
GTTTCCTTTT CTACAGATAT GTGGAGTGTG GGCGTCATCA CATACATGCT ACTCAGTGGC 
TTGTCCCCCT TTTTGGGAGA CAATGATGCC GAGACACTGA CCAACATCCT GGCGTGCCGG 
TGGGATTTAG AAGATGAGGA ATTTCAGGAT ATCTCGGAGG AGGCAAAGGA GTTCATCTCC 
AAGCTCCTGA TTAAGGAGAA GAGTTGGAGA ATAAGTGCCA GCGAAGCTCT CAAGCACCCA 
TGGTTGTCAG ACCACAAGCT CCACTCCAGG CTGAGTGCAC AGAAGAACTG CAACTCTGGT 
GTTCTGAACC TCACGACCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159502.1
CDS	64..1227
Translation	MLKVKRLEEISSCHSSNPLEKVAFFQCMEEVEKVKCFLEENSGDLDLQSGDNEAEENVWSNRALDERIIVKGGRTSALTDDIPAPAAPFDHRMVMAKHASVDNLYTVSKSEILGGGRFGQVHKCEEKATGLKLAAKIIKTRGAKDKEDVKNEISVMNQLDHVNLIQLYDAFESKHDIILVMEYVEGGELFDRIIDENCNLTELDTILFMKQICEGIRYMHQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSFSTDMWSVGVITYMLLSGLSPFLGDNDAETLTNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRISASEALKHPWLSDHKLHSRLSAQKNCNSGVLNLTTK

Target ORF information:

RefSeq Version	NM_001166030.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase family, member 4 (Mylk4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166030.1

ORF Insert Sequence:

ATGTTGAAGG TGAAGAGGCT GGAAGAAATC AGCTCGTGCC ACAGCAGCAA CCCGCTGGAG 
AAGGTGGCGT TTTTCCAGTG CATGGAGGAG GTGGAGAAGG TGAAATGCTT CCTGGAGGAG 
AATTCGGGGG ATCTGGACCT GCAGTCTGGA GACAATGAGG CTGAGGAAAA TGTATGGTCC 
AACCGTGCAC TAGATGAAAG GATAATTGTC AAAGGAGGAA GGACATCAGC CCTCACAGAT 
GACATCCCTG CTCCTGCCGC TCCATTTGAT CATCGGATGG TAATGGCCAA ACATGCTTCA 
GTGGACAATC TGTACACCGT GAGCAAATCT GAGATCCTAG GAGGGGGGCG TTTTGGTCAG 
GTACATAAGT GTGAAGAGAA AGCAACAGGC CTGAAGCTGG CTGCTAAAAT CATTAAGACT 
AGAGGTGCAA AGGACAAGGA GGATGTGAAG AACGAGATCA GCGTCATGAA CCAGCTGGAC 
CACGTGAACC TCATTCAGCT CTATGATGCC TTTGAGTCCA AGCATGACAT CATCCTTGTC 
ATGGAGTATG TGGAGGGTGG GGAGCTCTTT GACCGCATCA TTGATGAAAA CTGCAACTTG 
ACAGAGCTGG ACACCATCCT GTTCATGAAG CAGATCTGTG AGGGGATACG GTACATGCAC 
CAGATGTACA TCCTCCACTT GGATCTCAAG CCTGAGAACA TCCTGTGTGT GAATCGGGAT 
GCTAAGCAAA TAAAAATTAT TGATTTTGGA TTGGCCAGAA GATACAAGCC CAGAGAGAAG 
CTGAAGGTGA ACTTTGGAAC CCCAGAATTT CTTGCCCCTG AAGTTGTGAA CTATGATTTC 
GTTTCCTTTT CTACAGATAT GTGGAGTGTG GGCGTCATCA CATACATGCT ACTCAGTGGC 
TTGTCCCCCT TTTTGGGAGA CAATGATGCC GAGACACTGA CCAACATCCT GGCGTGCCGG 
TGGGATTTAG AAGATGAGGA ATTTCAGGAT ATCTCGGAGG AGGCAAAGGA GTTCATCTCC 
AAGCTCCTGA TTAAGGAGAA GAGTTGGAGA ATAAGTGCCA GCGAAGCTCT CAAGCACCCA 
TGGTTGTCAG ACCACAAGCT CCACTCCAGG CTGAGTGCAC AGAAGAACTG CAACTCTGGT 
GTTCTGAACC TCACGACCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk4 ( XM_006516668.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516731.1 Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu48387
Clone ID Related Accession (Same CDS sequence)	XM_006516668.3 , XM_006516668.4
Accession Version	XM_006516668.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase family member 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516668.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCACTC CTCAGAGTAG AGCAGGGAGC ACAGGGAACT TCAAAAGTGG CTGTGCTAGA 
CCAGGGCAGG GAAGGTGCCC ACAAAGCCAG GGACTTCACA GCCTCTCCCA GAGAATGGAC 
TCCACGCTTC GAAGAAAAGA CCTCTGGATT GTGGGTAGCG TGTGCCTTCT GTCGTCTTCG 
GTCTTCTGGC GCCTGTTTCG GTCAGTGGCG GCATGGGGAG GCCCCAAGCA GGAACCTGCA 
CTTGAGAATG CACAGGCTGA GGAAAATGTA TGGTCCAACC GTGCACTAGA TGAAAGGATA 
ATTGTCAAAG GAGGAAGGAC ATCAGCCCTC ACAGAGAAAA CTGACCAGAA TGACCAATTC 
TCTTCCGAGA AAGAAAAGAT TACCAAACTT AACAAGAAAA ATTCAGAGAA GACCAGTGCA 
TTAGAAGTGC AGAGGAGTTG TGTCATATGT CACGTGGAAA GACAAGACAA GATAGAATTT 
GTTCAGAGGA ATTCCACAGA GCTAAGAGAG AATGCAGGTG CAAAAGATGC TCCATGCATA 
GAAAGTCTGG AGAAACAAGA AAGCAACAAC CTGAGAGATA AACAAGACAC TGAAGATAAC 
AATGGAGGAG AAAATGGGAT TTCTTCTAAG GACGATTCCA GAGGAGTAGA AGCTGAGAAC 
AGAGCTTCAG CAGCTGCAAG TGGAGCAGTG CTAGACAAAC ACTCGACTGA AAGCCAGCAC 
ACTGAGGACA TGAATGGTGA CCCGTCCCAT GAAAGGTGCA TCAGTAATAT AAAGACCACT 
TCTACACCAG GAATCCTTGG AAATTCAGCA TCAGGATCCA CTTTCCAGAA TGCTGAACAG 
ACAGAGTCTA CTGGCAGCAG TAAGCGCAGG GTGGCAGAGG AGTGCTTCCT CAGTGAGGAC 
AACAAAAAGA GCAGAATAGA TGTAGTGAGA AGAGGGGAAG AGCCCACAGC TAGGACAGAG 
GAAACCAGGC AAGCCAGAAG CCTGGGTGAT AGACTCCAGG ATGGAATGAC CACCTCTAGA 
GAGCAAGACC TGGAGGTCCC ACTGGATGAC ATCCCTGCTC CTGCCGCTCC ATTTGATCAT 
CGGATGGTAA TGGCCAAACA TGCTTCAGTG GACAATCTGT ACACCGTGAG CAAATCTGAG 
ATCCTAGGAG GGGGGCGTTT TGGTCAGGTA CATAAGTGTG AAGAGAAAGC AACAGGCCTG 
AAGCTGGCTG CTAAAATCAT TAAGACTAGA GGTGCAAAGG ACAAGGAGGA TGTGAAGAAC 
GAGATCAGCG TCATGAACCA GCTGGACCAC GTGAACCTCA TTCAGCTCTA TGATGCCTTT 
GAGTCCAAGC ATGACATCAT CCTTGTCATG GAGTATGTGG AGGGTGGGGA GCTCTTTGAC 
CGCATCATTG ATGAAAACTG CAACTTGACA GAGCTGGACA CCATCCTGTT CATGAAGCAG 
ATCTGTGAGG GGATACGGTA CATGCACCAG ATGTACATCC TCCACTTGGA TCTCAAGCCT 
GAGAACATCC TGTGTGTGAA TCGGGATGCT AAGCAAATAA AAATTATTGA TTTTGGATTG 
GCCAGAAGAT ACAAGCCCAG AGAGAAGCTG AAGGTGAACT TTGGAACCCC AGAATTTCTT 
GCCCCTGAAG TTGTGAACTA TGATTTCGTT TCCTTTTCTA CAGATATGTG GAGTGTGGGC 
GTCATCACAT ACATGCTACT CAGTGGCTTG TCCCCCTTTT TGGGAGACAA TGATGCCGAG 
ACACTGACCA ACATCCTGGC GTGCCGGTGG GATTTAGAAG ATGAGGAATT TCAGGATATC 
TCGGAGGAGG CAAAGGAGTT CATCTCCAAG CTCCTGATTA AGGAGAAGAG TTGGAGAATA 
AGTGCCAGCG AAGCTCTCAA GCACCCATGG TTGTCAGACC ACAAGCTCCA CTCCAGGCTG 
AGTGCACAGA AGAACTGCAA CTCTGGTGTT CTGAACCTCA CGACCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516731.1
CDS	322..2292
Translation	MPTPQSRAGSTGNFKSGCARPGQGRCPQSQGLHSLSQRMDSTLRRKDLWIVGSVCLLSSSVFWRLFRSVAAWGGPKQEPALENAQAEENVWSNRALDERIIVKGGRTSALTEKTDQNDQFSSEKEKITKLNKKNSEKTSALEVQRSCVICHVERQDKIEFVQRNSTELRENAGAKDAPCIESLEKQESNNLRDKQDTEDNNGGENGISSKDDSRGVEAENRASAAASGAVLDKHSTESQHTEDMNGDPSHERCISNIKTTSTPGILGNSASGSTFQNAEQTESTGSSKRRVAEECFLSEDNKKSRIDVVRRGEEPTARTEETRQARSLGDRLQDGMTTSREQDLEVPLDDIPAPAAPFDHRMVMAKHASVDNLYTVSKSEILGGGRFGQVHKCEEKATGLKLAAKIIKTRGAKDKEDVKNEISVMNQLDHVNLIQLYDAFESKHDIILVMEYVEGGELFDRIIDENCNLTELDTILFMKQICEGIRYMHQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSFSTDMWSVGVITYMLLSGLSPFLGDNDAETLTNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRISASEALKHPWLSDHKLHSRLSAQKNCNSGVLNLTTK

Target ORF information:

RefSeq Version	XM_006516668.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516668.3

ORF Insert Sequence:

ATGCCCACTC CTCAGAGTAG AGCAGGGAGC ACAGGGAACT TCAAAAGTGG CTGTGCTAGA 
CCAGGGCAGG GAAGGTGCCC ACAAAGCCAG GGACTTCACA GCCTCTCCCA GAGAATGGAC 
TCCACGCTTC GAAGAAAAGA CCTCTGGATT GTGGGTAGCG TGTGCCTTCT GTCGTCTTCG 
GTCTTCTGGC GCCTGTTTCG GTCAGTGGCG GCATGGGGAG GCCCCAAGCA GGAACCTGCA 
CTTGAGAATG CACAGGCTGA GGAAAATGTA TGGTCCAACC GTGCACTAGA TGAAAGGATA 
ATTGTCAAAG GAGGAAGGAC ATCAGCCCTC ACAGAGAAAA CTGACCAGAA TGACCAATTC 
TCTTCCGAGA AAGAAAAGAT TACCAAACTT AACAAGAAAA ATTCAGAGAA GACCAGTGCA 
TTAGAAGTGC AGAGGAGTTG TGTCATATGT CACGTGGAAA GACAAGACAA GATAGAATTT 
GTTCAGAGGA ATTCCACAGA GCTAAGAGAG AATGCAGGTG CAAAAGATGC TCCATGCATA 
GAAAGTCTGG AGAAACAAGA AAGCAACAAC CTGAGAGATA AACAAGACAC TGAAGATAAC 
AATGGAGGAG AAAATGGGAT TTCTTCTAAG GACGATTCCA GAGGAGTAGA AGCTGAGAAC 
AGAGCTTCAG CAGCTGCAAG TGGAGCAGTG CTAGACAAAC ACTCGACTGA AAGCCAGCAC 
ACTGAGGACA TGAATGGTGA CCCGTCCCAT GAAAGGTGCA TCAGTAATAT AAAGACCACT 
TCTACACCAG GAATCCTTGG AAATTCAGCA TCAGGATCCA CTTTCCAGAA TGCTGAACAG 
ACAGAGTCTA CTGGCAGCAG TAAGCGCAGG GTGGCAGAGG AGTGCTTCCT CAGTGAGGAC 
AACAAAAAGA GCAGAATAGA TGTAGTGAGA AGAGGGGAAG AGCCCACAGC TAGGACAGAG 
GAAACCAGGC AAGCCAGAAG CCTGGGTGAT AGACTCCAGG ATGGAATGAC CACCTCTAGA 
GAGCAAGACC TGGAGGTCCC ACTGGATGAC ATCCCTGCTC CTGCCGCTCC ATTTGATCAT 
CGGATGGTAA TGGCCAAACA TGCTTCAGTG GACAATCTGT ACACCGTGAG CAAATCTGAG 
ATCCTAGGAG GGGGGCGTTT TGGTCAGGTA CATAAGTGTG AAGAGAAAGC AACAGGCCTG 
AAGCTGGCTG CTAAAATCAT TAAGACTAGA GGTGCAAAGG ACAAGGAGGA TGTGAAGAAC 
GAGATCAGCG TCATGAACCA GCTGGACCAC GTGAACCTCA TTCAGCTCTA TGATGCCTTT 
GAGTCCAAGC ATGACATCAT CCTTGTCATG GAGTATGTGG AGGGTGGGGA GCTCTTTGAC 
CGCATCATTG ATGAAAACTG CAACTTGACA GAGCTGGACA CCATCCTGTT CATGAAGCAG 
ATCTGTGAGG GGATACGGTA CATGCACCAG ATGTACATCC TCCACTTGGA TCTCAAGCCT 
GAGAACATCC TGTGTGTGAA TCGGGATGCT AAGCAAATAA AAATTATTGA TTTTGGATTG 
GCCAGAAGAT ACAAGCCCAG AGAGAAGCTG AAGGTGAACT TTGGAACCCC AGAATTTCTT 
GCCCCTGAAG TTGTGAACTA TGATTTCGTT TCCTTTTCTA CAGATATGTG GAGTGTGGGC 
GTCATCACAT ACATGCTACT CAGTGGCTTG TCCCCCTTTT TGGGAGACAA TGATGCCGAG 
ACACTGACCA ACATCCTGGC GTGCCGGTGG GATTTAGAAG ATGAGGAATT TCAGGATATC 
TCGGAGGAGG CAAAGGAGTT CATCTCCAAG CTCCTGATTA AGGAGAAGAG TTGGAGAATA 
AGTGCCAGCG AAGCTCTCAA GCACCCATGG TTGTCAGACC ACAAGCTCCA CTCCAGGCTG 
AGTGCACAGA AGAACTGCAA CTCTGGTGTT CTGAACCTCA CGACCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk4 ( XM_006516670.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516733.1 Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu48389
Clone ID Related Accession (Same CDS sequence)	XM_006516670.3
Accession Version	XM_006516670.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase family member 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAA AAGAGAAGAA AGTGATTCCA CTCAAGAGCC ACGTGCAGCA TCCTACTCAA 
GAGAAGGGAG TGCTGAGCAG TCATGATTGT GTCCTCATTT ACAAATCTGA GACAGAGGGA 
GATGAAAGCC CCACTCCTGA CAGCAGCCTG ATCAGGCTCA CCACCAGTAT GTTGAAGGTG 
AAGAGGCTGG AAGAAATCAG CTCGTGCCAC AGCAGCAACC CGCTGGAGAA GGTGGCGTTT 
TTCCAGTGCA TGGAGGAGGT GGAGAAGGTG AAATGCTTCC TGGAGGAGAA TTCGGGGGAT 
CTGGACCTGC AGTCTGGAGA CAATGAGGCT GAGGAAAATG TATGGTCCAA CCGTGCACTA 
GATGAAAGGA TAATTGTCAA AGGAGGAAGG ACATCAGCCC TCACAGAGAA AACTGACCAG 
AATGACCAAT TCTCTTCCGA GAAAGAAAAG ATTACCAAAC TTAACAAGAA AAATTCAGAG 
AAGACCAGTG CATTAGAAGT GCAGAGGAGT TGTGTCATAT GTCACGTGGA AAGACAAGAC 
AAGATAGAAT TTGTTCAGAG GAATTCCACA GAGCTAAGAG AGAATGCAGG TGCAAAAGAT 
GCTCCATGCA TAGAAAGTCT GGAGAAACAA GAAAGCAACA ACCTGAGAGA TAAACAAGAC 
ACTGAAGATA ACAATGGAGG AGAAAATGGG ATTTCTTCTA AGGACGATTC CAGAGGAGTA 
GAAGCTGAGA ACAGAGCTTC AGCAGCTGCA AGTGGAGCAG TGCTAGACAA ACACTCGACT 
GAAAGCCAGC ACACTGAGGA CATGAATGGT GACCCGTCCC ATGAAAGGTG CATCAGTAAT 
ATAAAGACCA CTTCTACACC AGGAATCCTT GGAAATTCAG CATCAGGATC CACTTTCCAG 
AATGCTGAAC AGACAGAGTC TACTGGCAGC AATGACATCC CTGCTCCTGC CGCTCCATTT 
GATCATCGGA TGGTAATGGC CAAACATGCT TCAGTGGACA ATCTGTACAC CGTGAGCAAA 
TCTGAGATCC TAGGAGGGGG GCGTTTTGGT CAGGTACATA AGTGTGAAGA GAAAGCAACA 
GGCCTGAAGC TGGCTGCTAA AATCATTAAG ACTAGAGGTG CAAAGGACAA GGAGGATGTG 
AAGAACGAGA TCAGCGTCAT GAACCAGCTG GACCACGTGA ACCTCATTCA GCTCTATGAT 
GCCTTTGAGT CCAAGCATGA CATCATCCTT GTCATGGAGT ATGTGGAGGG TGGGGAGCTC 
TTTGACCGCA TCATTGATGA AAACTGCAAC TTGACAGAGC TGGACACCAT CCTGTTCATG 
AAGCAGATCT GTGAGGGGAT ACGGTACATG CACCAGATGT ACATCCTCCA CTTGGATCTC 
AAGCCTGAGA ACATCCTGTG TGTGAATCGG GATGCTAAGC AAATAAAAAT TATTGATTTT 
GGATTGGCCA GAAGATACAA GCCCAGAGAG AAGCTGAAGG TGAACTTTGG AACCCCAGAA 
TTTCTTGCCC CTGAAGTTGT GAACTATGAT TTCGTTTCCT TTTCTACAGA TATGTGGAGT 
GTGGGCGTCA TCACATACAT GCTACTCAGT GGCTTGTCCC CCTTTTTGGG AGACAATGAT 
GCCGAGACAC TGACCAACAT CCTGGCGTGC CGGTGGGATT TAGAAGATGA GGAATTTCAG 
GATATCTCGG AGGAGGCAAA GGAGTTCATC TCCAAGCTCC TGATTAAGGA GAAGAGTTGG 
AGAATAAGTG CCAGCGAAGC TCTCAAGCAC CCATGGTTGT CAGACCACAA GCTCCACTCC 
AGGCTGAGTG CACAGAAGAA CTGCAACTCT GGTGTTCTGA ACCTCACGAC CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516733.1
CDS	22..1878
Translation	MGGKEKKVIPLKSHVQHPTQEKGVLSSHDCVLIYKSETEGDESPTPDSSLIRLTTSMLKVKRLEEISSCHSSNPLEKVAFFQCMEEVEKVKCFLEENSGDLDLQSGDNEAEENVWSNRALDERIIVKGGRTSALTEKTDQNDQFSSEKEKITKLNKKNSEKTSALEVQRSCVICHVERQDKIEFVQRNSTELRENAGAKDAPCIESLEKQESNNLRDKQDTEDNNGGENGISSKDDSRGVEAENRASAAASGAVLDKHSTESQHTEDMNGDPSHERCISNIKTTSTPGILGNSASGSTFQNAEQTESTGSNDIPAPAAPFDHRMVMAKHASVDNLYTVSKSEILGGGRFGQVHKCEEKATGLKLAAKIIKTRGAKDKEDVKNEISVMNQLDHVNLIQLYDAFESKHDIILVMEYVEGGELFDRIIDENCNLTELDTILFMKQICEGIRYMHQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSFSTDMWSVGVITYMLLSGLSPFLGDNDAETLTNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRISASEALKHPWLSDHKLHSRLSAQKNCNSGVLNLTTK

Target ORF information:

RefSeq Version	XM_006516670.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516670.3

ORF Insert Sequence:

ATGGGAGGAA AAGAGAAGAA AGTGATTCCA CTCAAGAGCC ACGTGCAGCA TCCTACTCAA 
GAGAAGGGAG TGCTGAGCAG TCATGATTGT GTCCTCATTT ACAAATCTGA GACAGAGGGA 
GATGAAAGCC CCACTCCTGA CAGCAGCCTG ATCAGGCTCA CCACCAGTAT GTTGAAGGTG 
AAGAGGCTGG AAGAAATCAG CTCGTGCCAC AGCAGCAACC CGCTGGAGAA GGTGGCGTTT 
TTCCAGTGCA TGGAGGAGGT GGAGAAGGTG AAATGCTTCC TGGAGGAGAA TTCGGGGGAT 
CTGGACCTGC AGTCTGGAGA CAATGAGGCT GAGGAAAATG TATGGTCCAA CCGTGCACTA 
GATGAAAGGA TAATTGTCAA AGGAGGAAGG ACATCAGCCC TCACAGAGAA AACTGACCAG 
AATGACCAAT TCTCTTCCGA GAAAGAAAAG ATTACCAAAC TTAACAAGAA AAATTCAGAG 
AAGACCAGTG CATTAGAAGT GCAGAGGAGT TGTGTCATAT GTCACGTGGA AAGACAAGAC 
AAGATAGAAT TTGTTCAGAG GAATTCCACA GAGCTAAGAG AGAATGCAGG TGCAAAAGAT 
GCTCCATGCA TAGAAAGTCT GGAGAAACAA GAAAGCAACA ACCTGAGAGA TAAACAAGAC 
ACTGAAGATA ACAATGGAGG AGAAAATGGG ATTTCTTCTA AGGACGATTC CAGAGGAGTA 
GAAGCTGAGA ACAGAGCTTC AGCAGCTGCA AGTGGAGCAG TGCTAGACAA ACACTCGACT 
GAAAGCCAGC ACACTGAGGA CATGAATGGT GACCCGTCCC ATGAAAGGTG CATCAGTAAT 
ATAAAGACCA CTTCTACACC AGGAATCCTT GGAAATTCAG CATCAGGATC CACTTTCCAG 
AATGCTGAAC AGACAGAGTC TACTGGCAGC AATGACATCC CTGCTCCTGC CGCTCCATTT 
GATCATCGGA TGGTAATGGC CAAACATGCT TCAGTGGACA ATCTGTACAC CGTGAGCAAA 
TCTGAGATCC TAGGAGGGGG GCGTTTTGGT CAGGTACATA AGTGTGAAGA GAAAGCAACA 
GGCCTGAAGC TGGCTGCTAA AATCATTAAG ACTAGAGGTG CAAAGGACAA GGAGGATGTG 
AAGAACGAGA TCAGCGTCAT GAACCAGCTG GACCACGTGA ACCTCATTCA GCTCTATGAT 
GCCTTTGAGT CCAAGCATGA CATCATCCTT GTCATGGAGT ATGTGGAGGG TGGGGAGCTC 
TTTGACCGCA TCATTGATGA AAACTGCAAC TTGACAGAGC TGGACACCAT CCTGTTCATG 
AAGCAGATCT GTGAGGGGAT ACGGTACATG CACCAGATGT ACATCCTCCA CTTGGATCTC 
AAGCCTGAGA ACATCCTGTG TGTGAATCGG GATGCTAAGC AAATAAAAAT TATTGATTTT 
GGATTGGCCA GAAGATACAA GCCCAGAGAG AAGCTGAAGG TGAACTTTGG AACCCCAGAA 
TTTCTTGCCC CTGAAGTTGT GAACTATGAT TTCGTTTCCT TTTCTACAGA TATGTGGAGT 
GTGGGCGTCA TCACATACAT GCTACTCAGT GGCTTGTCCC CCTTTTTGGG AGACAATGAT 
GCCGAGACAC TGACCAACAT CCTGGCGTGC CGGTGGGATT TAGAAGATGA GGAATTTCAG 
GATATCTCGG AGGAGGCAAA GGAGTTCATC TCCAAGCTCC TGATTAAGGA GAAGAGTTGG 
AGAATAAGTG CCAGCGAAGC TCTCAAGCAC CCATGGTTGT CAGACCACAA GCTCCACTCC 
AGGCTGAGTG CACAGAAGAA CTGCAACTCT GGTGTTCTGA ACCTCACGAC CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk4 ( XM_006516666.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516729.1 Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X1, mRNA.

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CloneID	OMu48385
Clone ID Related Accession (Same CDS sequence)	XM_006516666.3
Accession Version	XM_006516666.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516666.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGAA AAGAGAAGAA AGTGATTCCA CTCAAGAGCC ACGTGCAGCA TCCTACTCAA 
GAGAAGGGAG TGCTGAGCAG TCATGATTGT GTCCTCATTT ACAAATCTGA GACAGAGGGA 
GATGAAAGCC CCACTCCTGA CAGCAGCCTG ATCAGGCTCA CCACCAGTAT GTTGAAGGTG 
AAGAGGCTGG AAGAAATCAG CTCGTGCCAC AGCAGCAACC CGCTGGAGAA GGTGGCGTTT 
TTCCAGTGCA TGGAGGAGGT GGAGAAGGTG AAATGCTTCC TGGAGGAGAA TTCGGGGGAT 
CTGGACCTGC AGTCTGGAGA CAATGAGGCT GAGGAAAATG TATGGTCCAA CCGTGCACTA 
GATGAAAGGA TAATTGTCAA AGGAGGAAGG ACATCAGCCC TCACAGAGAA AACTGACCAG 
AATGACCAAT TCTCTTCCGA GAAAGAAAAG ATTACCAAAC TTAACAAGAA AAATTCAGAG 
AAGACCAGTG CATTAGAAGT GCAGAGGAGT TGTGTCATAT GTCACGTGGA AAGACAAGAC 
AAGATAGAAT TTGTTCAGAG GAATTCCACA GAGCTAAGAG AGAATGCAGG TGCAAAAGAT 
GCTCCATGCA TAGAAAGTCT GGAGAAACAA GAAAGCAACA ACCTGAGAGA TAAACAAGAC 
ACTGAAGATA ACAATGGAGG AGAAAATGGG ATTTCTTCTA AGGACGATTC CAGAGGAGTA 
GAAGCTGAGA ACAGAGCTTC AGCAGCTGCA AGTGGAGCAG TGCTAGACAA ACACTCGACT 
GAAAGCCAGC ACACTGAGGA CATGAATGGT GACCCGTCCC ATGAAAGGTG CATCAGTAAT 
ATAAAGACCA CTTCTACACC AGGAATCCTT GGAAATTCAG CATCAGGATC CACTTTCCAG 
AATGCTGAAC AGACAGAGTC TACTGGCAGC AGTAAGCGCA GGGTGGCAGA GGAGTGCTTC 
CTCAGTGAGG ACAACAAAAA GAGCAGAATA GATGTAGTGA GAAGAGGGGA AGAGCCCACA 
GCTAGGACAG AGGAAACCAG GCAAGCCAGA AGCCTGGGTG ATAGACTCCA GGATGGAATG 
ACCACCTCTA GAGAGCAAGA CCTGGAGGTC CCACTGGATG ACATCCCTGC TCCTGCCGCT 
CCATTTGATC ATCGGATGGT AATGGCCAAA CATGCTTCAG TGGACAATCT GTACACCGTG 
AGCAAATCTG AGATCCTAGG AGGGGGGCGT TTTGGTCAGG TACATAAGTG TGAAGAGAAA 
GCAACAGGCC TGAAGCTGGC TGCTAAAATC ATTAAGACTA GAGGTGCAAA GGACAAGGAG 
GATGTGAAGA ACGAGATCAG CGTCATGAAC CAGCTGGACC ACGTGAACCT CATTCAGCTC 
TATGATGCCT TTGAGTCCAA GCATGACATC ATCCTTGTCA TGGAGTATGT GGAGGGTGGG 
GAGCTCTTTG ACCGCATCAT TGATGAAAAC TGCAACTTGA CAGAGCTGGA CACCATCCTG 
TTCATGAAGC AGATCTGTGA GGGGATACGG TACATGCACC AGATGTACAT CCTCCACTTG 
GATCTCAAGC CTGAGAACAT CCTGTGTGTG AATCGGGATG CTAAGCAAAT AAAAATTATT 
GATTTTGGAT TGGCCAGAAG ATACAAGCCC AGAGAGAAGC TGAAGGTGAA CTTTGGAACC 
CCAGAATTTC TTGCCCCTGA AGTTGTGAAC TATGATTTCG TTTCCTTTTC TACAGATATG 
TGGAGTGTGG GCGTCATCAC ATACATGCTA CTCAGTGGCT TGTCCCCCTT TTTGGGAGAC 
AATGATGCCG AGACACTGAC CAACATCCTG GCGTGCCGGT GGGATTTAGA AGATGAGGAA 
TTTCAGGATA TCTCGGAGGA GGCAAAGGAG TTCATCTCCA AGCTCCTGAT TAAGGAGAAG 
AGTTGGAGAA TAAGTGCCAG CGAAGCTCTC AAGCACCCAT GGTTGTCAGA CCACAAGCTC 
CACTCCAGGC TGAGTGCACA GAAGAACTGC AACTCTGGTG TTCTGAACCT CACGACCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516729.1
CDS	22..2064
Translation	MGGKEKKVIPLKSHVQHPTQEKGVLSSHDCVLIYKSETEGDESPTPDSSLIRLTTSMLKVKRLEEISSCHSSNPLEKVAFFQCMEEVEKVKCFLEENSGDLDLQSGDNEAEENVWSNRALDERIIVKGGRTSALTEKTDQNDQFSSEKEKITKLNKKNSEKTSALEVQRSCVICHVERQDKIEFVQRNSTELRENAGAKDAPCIESLEKQESNNLRDKQDTEDNNGGENGISSKDDSRGVEAENRASAAASGAVLDKHSTESQHTEDMNGDPSHERCISNIKTTSTPGILGNSASGSTFQNAEQTESTGSSKRRVAEECFLSEDNKKSRIDVVRRGEEPTARTEETRQARSLGDRLQDGMTTSREQDLEVPLDDIPAPAAPFDHRMVMAKHASVDNLYTVSKSEILGGGRFGQVHKCEEKATGLKLAAKIIKTRGAKDKEDVKNEISVMNQLDHVNLIQLYDAFESKHDIILVMEYVEGGELFDRIIDENCNLTELDTILFMKQICEGIRYMHQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSFSTDMWSVGVITYMLLSGLSPFLGDNDAETLTNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRISASEALKHPWLSDHKLHSRLSAQKNCNSGVLNLTTK

Target ORF information:

RefSeq Version	XM_006516666.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516666.3

ORF Insert Sequence:

ATGGGAGGAA AAGAGAAGAA AGTGATTCCA CTCAAGAGCC ACGTGCAGCA TCCTACTCAA 
GAGAAGGGAG TGCTGAGCAG TCATGATTGT GTCCTCATTT ACAAATCTGA GACAGAGGGA 
GATGAAAGCC CCACTCCTGA CAGCAGCCTG ATCAGGCTCA CCACCAGTAT GTTGAAGGTG 
AAGAGGCTGG AAGAAATCAG CTCGTGCCAC AGCAGCAACC CGCTGGAGAA GGTGGCGTTT 
TTCCAGTGCA TGGAGGAGGT GGAGAAGGTG AAATGCTTCC TGGAGGAGAA TTCGGGGGAT 
CTGGACCTGC AGTCTGGAGA CAATGAGGCT GAGGAAAATG TATGGTCCAA CCGTGCACTA 
GATGAAAGGA TAATTGTCAA AGGAGGAAGG ACATCAGCCC TCACAGAGAA AACTGACCAG 
AATGACCAAT TCTCTTCCGA GAAAGAAAAG ATTACCAAAC TTAACAAGAA AAATTCAGAG 
AAGACCAGTG CATTAGAAGT GCAGAGGAGT TGTGTCATAT GTCACGTGGA AAGACAAGAC 
AAGATAGAAT TTGTTCAGAG GAATTCCACA GAGCTAAGAG AGAATGCAGG TGCAAAAGAT 
GCTCCATGCA TAGAAAGTCT GGAGAAACAA GAAAGCAACA ACCTGAGAGA TAAACAAGAC 
ACTGAAGATA ACAATGGAGG AGAAAATGGG ATTTCTTCTA AGGACGATTC CAGAGGAGTA 
GAAGCTGAGA ACAGAGCTTC AGCAGCTGCA AGTGGAGCAG TGCTAGACAA ACACTCGACT 
GAAAGCCAGC ACACTGAGGA CATGAATGGT GACCCGTCCC ATGAAAGGTG CATCAGTAAT 
ATAAAGACCA CTTCTACACC AGGAATCCTT GGAAATTCAG CATCAGGATC CACTTTCCAG 
AATGCTGAAC AGACAGAGTC TACTGGCAGC AGTAAGCGCA GGGTGGCAGA GGAGTGCTTC 
CTCAGTGAGG ACAACAAAAA GAGCAGAATA GATGTAGTGA GAAGAGGGGA AGAGCCCACA 
GCTAGGACAG AGGAAACCAG GCAAGCCAGA AGCCTGGGTG ATAGACTCCA GGATGGAATG 
ACCACCTCTA GAGAGCAAGA CCTGGAGGTC CCACTGGATG ACATCCCTGC TCCTGCCGCT 
CCATTTGATC ATCGGATGGT AATGGCCAAA CATGCTTCAG TGGACAATCT GTACACCGTG 
AGCAAATCTG AGATCCTAGG AGGGGGGCGT TTTGGTCAGG TACATAAGTG TGAAGAGAAA 
GCAACAGGCC TGAAGCTGGC TGCTAAAATC ATTAAGACTA GAGGTGCAAA GGACAAGGAG 
GATGTGAAGA ACGAGATCAG CGTCATGAAC CAGCTGGACC ACGTGAACCT CATTCAGCTC 
TATGATGCCT TTGAGTCCAA GCATGACATC ATCCTTGTCA TGGAGTATGT GGAGGGTGGG 
GAGCTCTTTG ACCGCATCAT TGATGAAAAC TGCAACTTGA CAGAGCTGGA CACCATCCTG 
TTCATGAAGC AGATCTGTGA GGGGATACGG TACATGCACC AGATGTACAT CCTCCACTTG 
GATCTCAAGC CTGAGAACAT CCTGTGTGTG AATCGGGATG CTAAGCAAAT AAAAATTATT 
GATTTTGGAT TGGCCAGAAG ATACAAGCCC AGAGAGAAGC TGAAGGTGAA CTTTGGAACC 
CCAGAATTTC TTGCCCCTGA AGTTGTGAAC TATGATTTCG TTTCCTTTTC TACAGATATG 
TGGAGTGTGG GCGTCATCAC ATACATGCTA CTCAGTGGCT TGTCCCCCTT TTTGGGAGAC 
AATGATGCCG AGACACTGAC CAACATCCTG GCGTGCCGGT GGGATTTAGA AGATGAGGAA 
TTTCAGGATA TCTCGGAGGA GGCAAAGGAG TTCATCTCCA AGCTCCTGAT TAAGGAGAAG 
AGTTGGAGAA TAAGTGCCAG CGAAGCTCTC AAGCACCCAT GGTTGTCAGA CCACAAGCTC 
CACTCCAGGC TGAGTGCACA GAAGAACTGC AACTCTGGTG TTCTGAACCT CACGACCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk4 ( XM_006516667.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516730.1 Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X2, mRNA.

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CloneID	OMu48386
Clone ID Related Accession (Same CDS sequence)	XM_006516667.2 , XM_006516667.3
Accession Version	XM_006516667.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006516667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCT ACAGATGTCA AGAGAAGGGA GTGCTGAGCA GTCATGATTG TGTCCTCATT 
TACAAATCTG AGACAGAGGG AGATGAAAGC CCCACTCCTG ACAGCAGCCT GATCAGGCTC 
ACCACCAGTA TGTTGAAGGT GAAGAGGCTG GAAGAAATCA GCTCGTGCCA CAGCAGCAAC 
CCGCTGGAGA AGGTGGCGTT TTTCCAGTGC ATGGAGGAGG TGGAGAAGGT GAAATGCTTC 
CTGGAGGAGA ATTCGGGGGA TCTGGACCTG CAGTCTGGAG ACAATGAGGC TGAGGAAAAT 
GTATGGTCCA ACCGTGCACT AGATGAAAGG ATAATTGTCA AAGGAGGAAG GACATCAGCC 
CTCACAGAGA AAACTGACCA GAATGACCAA TTCTCTTCCG AGAAAGAAAA GATTACCAAA 
CTTAACAAGA AAAATTCAGA GAAGACCAGT GCATTAGAAG TGCAGAGGAG TTGTGTCATA 
TGTCACGTGG AAAGACAAGA CAAGATAGAA TTTGTTCAGA GGAATTCCAC AGAGCTAAGA 
GAGAATGCAG GTGCAAAAGA TGCTCCATGC ATAGAAAGTC TGGAGAAACA AGAAAGCAAC 
AACCTGAGAG ATAAACAAGA CACTGAAGAT AACAATGGAG GAGAAAATGG GATTTCTTCT 
AAGGACGATT CCAGAGGAGT AGAAGCTGAG AACAGAGCTT CAGCAGCTGC AAGTGGAGCA 
GTGCTAGACA AACACTCGAC TGAAAGCCAG CACACTGAGG ACATGAATGG TGACCCGTCC 
CATGAAAGGT GCATCAGTAA TATAAAGACC ACTTCTACAC CAGGAATCCT TGGAAATTCA 
GCATCAGGAT CCACTTTCCA GAATGCTGAA CAGACAGAGT CTACTGGCAG CAGTAAGCGC 
AGGGTGGCAG AGGAGTGCTT CCTCAGTGAG GACAACAAAA AGAGCAGAAT AGATGTAGTG 
AGAAGAGGGG AAGAGCCCAC AGCTAGGACA GAGGAAACCA GGCAAGCCAG AAGCCTGGGT 
GATAGACTCC AGGATGGAAT GACCACCTCT AGAGAGCAAG ACCTGGAGGT CCCACTGGAT 
GACATCCCTG CTCCTGCCGC TCCATTTGAT CATCGGATGG TAATGGCCAA ACATGCTTCA 
GTGGACAATC TGTACACCGT GAGCAAATCT GAGATCCTAG GAGGGGGGCG TTTTGGTCAG 
GTACATAAGT GTGAAGAGAA AGCAACAGGC CTGAAGCTGG CTGCTAAAAT CATTAAGACT 
AGAGGTGCAA AGGACAAGGA GGATGTGAAG AACGAGATCA GCGTCATGAA CCAGCTGGAC 
CACGTGAACC TCATTCAGCT CTATGATGCC TTTGAGTCCA AGCATGACAT CATCCTTGTC 
ATGGAGTATG TGGAGGGTGG GGAGCTCTTT GACCGCATCA TTGATGAAAA CTGCAACTTG 
ACAGAGCTGG ACACCATCCT GTTCATGAAG CAGATCTGTG AGGGGATACG GTACATGCAC 
CAGATGTACA TCCTCCACTT GGATCTCAAG CCTGAGAACA TCCTGTGTGT GAATCGGGAT 
GCTAAGCAAA TAAAAATTAT TGATTTTGGA TTGGCCAGAA GATACAAGCC CAGAGAGAAG 
CTGAAGGTGA ACTTTGGAAC CCCAGAATTT CTTGCCCCTG AAGTTGTGAA CTATGATTTC 
GTTTCCTTTT CTACAGATAT GTGGAGTGTG GGCGTCATCA CATACATGCT ACTCAGTGGC 
TTGTCCCCCT TTTTGGGAGA CAATGATGCC GAGACACTGA CCAACATCCT GGCGTGCCGG 
TGGGATTTAG AAGATGAGGA ATTTCAGGAT ATCTCGGAGG AGGCAAAGGA GTTCATCTCC 
AAGCTCCTGA TTAAGGAGAA GAGTTGGAGA ATAAGTGCCA GCGAAGCTCT CAAGCACCCA 
TGGTTGTCAG ACCACAAGCT CCACTCCAGG CTGAGTGCAC AGAAGAACTG CAACTCTGGT 
GTTCTGAACC TCACGACCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516730.1
CDS	231..2234
Translation	MSSYRCQEKGVLSSHDCVLIYKSETEGDESPTPDSSLIRLTTSMLKVKRLEEISSCHSSNPLEKVAFFQCMEEVEKVKCFLEENSGDLDLQSGDNEAEENVWSNRALDERIIVKGGRTSALTEKTDQNDQFSSEKEKITKLNKKNSEKTSALEVQRSCVICHVERQDKIEFVQRNSTELRENAGAKDAPCIESLEKQESNNLRDKQDTEDNNGGENGISSKDDSRGVEAENRASAAASGAVLDKHSTESQHTEDMNGDPSHERCISNIKTTSTPGILGNSASGSTFQNAEQTESTGSSKRRVAEECFLSEDNKKSRIDVVRRGEEPTARTEETRQARSLGDRLQDGMTTSREQDLEVPLDDIPAPAAPFDHRMVMAKHASVDNLYTVSKSEILGGGRFGQVHKCEEKATGLKLAAKIIKTRGAKDKEDVKNEISVMNQLDHVNLIQLYDAFESKHDIILVMEYVEGGELFDRIIDENCNLTELDTILFMKQICEGIRYMHQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSFSTDMWSVGVITYMLLSGLSPFLGDNDAETLTNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRISASEALKHPWLSDHKLHSRLSAQKNCNSGVLNLTTK

Target ORF information:

RefSeq Version	XM_006516667.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516667.2

ORF Insert Sequence:

ATGTCCTCCT ACAGATGTCA AGAGAAGGGA GTGCTGAGCA GTCATGATTG TGTCCTCATT 
TACAAATCTG AGACAGAGGG AGATGAAAGC CCCACTCCTG ACAGCAGCCT GATCAGGCTC 
ACCACCAGTA TGTTGAAGGT GAAGAGGCTG GAAGAAATCA GCTCGTGCCA CAGCAGCAAC 
CCGCTGGAGA AGGTGGCGTT TTTCCAGTGC ATGGAGGAGG TGGAGAAGGT GAAATGCTTC 
CTGGAGGAGA ATTCGGGGGA TCTGGACCTG CAGTCTGGAG ACAATGAGGC TGAGGAAAAT 
GTATGGTCCA ACCGTGCACT AGATGAAAGG ATAATTGTCA AAGGAGGAAG GACATCAGCC 
CTCACAGAGA AAACTGACCA GAATGACCAA TTCTCTTCCG AGAAAGAAAA GATTACCAAA 
CTTAACAAGA AAAATTCAGA GAAGACCAGT GCATTAGAAG TGCAGAGGAG TTGTGTCATA 
TGTCACGTGG AAAGACAAGA CAAGATAGAA TTTGTTCAGA GGAATTCCAC AGAGCTAAGA 
GAGAATGCAG GTGCAAAAGA TGCTCCATGC ATAGAAAGTC TGGAGAAACA AGAAAGCAAC 
AACCTGAGAG ATAAACAAGA CACTGAAGAT AACAATGGAG GAGAAAATGG GATTTCTTCT 
AAGGACGATT CCAGAGGAGT AGAAGCTGAG AACAGAGCTT CAGCAGCTGC AAGTGGAGCA 
GTGCTAGACA AACACTCGAC TGAAAGCCAG CACACTGAGG ACATGAATGG TGACCCGTCC 
CATGAAAGGT GCATCAGTAA TATAAAGACC ACTTCTACAC CAGGAATCCT TGGAAATTCA 
GCATCAGGAT CCACTTTCCA GAATGCTGAA CAGACAGAGT CTACTGGCAG CAGTAAGCGC 
AGGGTGGCAG AGGAGTGCTT CCTCAGTGAG GACAACAAAA AGAGCAGAAT AGATGTAGTG 
AGAAGAGGGG AAGAGCCCAC AGCTAGGACA GAGGAAACCA GGCAAGCCAG AAGCCTGGGT 
GATAGACTCC AGGATGGAAT GACCACCTCT AGAGAGCAAG ACCTGGAGGT CCCACTGGAT 
GACATCCCTG CTCCTGCCGC TCCATTTGAT CATCGGATGG TAATGGCCAA ACATGCTTCA 
GTGGACAATC TGTACACCGT GAGCAAATCT GAGATCCTAG GAGGGGGGCG TTTTGGTCAG 
GTACATAAGT GTGAAGAGAA AGCAACAGGC CTGAAGCTGG CTGCTAAAAT CATTAAGACT 
AGAGGTGCAA AGGACAAGGA GGATGTGAAG AACGAGATCA GCGTCATGAA CCAGCTGGAC 
CACGTGAACC TCATTCAGCT CTATGATGCC TTTGAGTCCA AGCATGACAT CATCCTTGTC 
ATGGAGTATG TGGAGGGTGG GGAGCTCTTT GACCGCATCA TTGATGAAAA CTGCAACTTG 
ACAGAGCTGG ACACCATCCT GTTCATGAAG CAGATCTGTG AGGGGATACG GTACATGCAC 
CAGATGTACA TCCTCCACTT GGATCTCAAG CCTGAGAACA TCCTGTGTGT GAATCGGGAT 
GCTAAGCAAA TAAAAATTAT TGATTTTGGA TTGGCCAGAA GATACAAGCC CAGAGAGAAG 
CTGAAGGTGA ACTTTGGAAC CCCAGAATTT CTTGCCCCTG AAGTTGTGAA CTATGATTTC 
GTTTCCTTTT CTACAGATAT GTGGAGTGTG GGCGTCATCA CATACATGCT ACTCAGTGGC 
TTGTCCCCCT TTTTGGGAGA CAATGATGCC GAGACACTGA CCAACATCCT GGCGTGCCGG 
TGGGATTTAG AAGATGAGGA ATTTCAGGAT ATCTCGGAGG AGGCAAAGGA GTTCATCTCC 
AAGCTCCTGA TTAAGGAGAA GAGTTGGAGA ATAAGTGCCA GCGAAGCTCT CAAGCACCCA 
TGGTTGTCAG ACCACAAGCT CCACTCCAGG CTGAGTGCAC AGAAGAACTG CAACTCTGGT 
GTTCTGAACC TCACGACCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk4 ( NM_001368880.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001355809.1 Mus musculus myosin light chain kinase family, member 4 (Mylk4), mRNA.

Price & Availability↑

CloneID	OMu84643
Clone ID Related Accession (Same CDS sequence)	NM_001368880.1
Accession Version	NM_001368880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase family member 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645808.13. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGTTGAAGG TGAAGAGGCT GGAAGAAATC AGCTCGTGCC ACAGCAGCAA CCCGCTGGAG 
AAGGTGGCGT TTTTCCAGTG CATGGAGGAG GTGGAGAAGG TGAAATGCTT CCTGGAGGAG 
AATTCGGGGG ATCTGGACCT GCAGTCTGGA GACAATGAGG CTGAGGAAAA TGTATGGTCC 
AACCGTGCAC TAGATGAAAG GATAATTGTC AAAGGAGGAA GGACATCAGC CCTCACAGAG 
AAAACTGACC AGAATGACCA ATTCTCTTCC GAGAAAGAAA AGATTACCAA ACTTAACAAG 
AAAAATTCAG AGAAGACCAG TGCATTAGAA GTGCAGAGGA GTTGTGTCAT ATGTCACGTG 
GAAAGACAAG ACAAGATAGA ATTTGTTCAG AGGAATTCCA CAGAGCTAAG AGAGAATGCA 
GGTGCAAAAG ATGCTCCATG CATAGAAAGT CTGGAGAAAC AAGAAAGCAA CAACCTGAGA 
GATAAACAAG ACACTGAAGA TAACAATGGA GGAGAAAATG GGATTTCTTC TAAGGACGAT 
TCCAGAGGAG TAGAAGCTGA GAACAGAGCT TCAGCAGCTG CAAGTGGAGC AGTGCTAGAC 
AAACACTCGA CTGAAAGCCA GCACACTGAG GACATGAATG GTGACCCGTC CCATGAAAGG 
TGCATCAGTA ATATAAAGAC CACTTCTACA CCAGGAATCC TTGGAAATTC AGCATCAGGA 
TCCACTTTCC AGAATGCTGA ACAGACAGAG TCTACTGGCA GCAGTAAGCG CAGGGTGGCA 
GAGGAGTGCT TCCTCAGTGA GGACAACAAA AAGAGCAGAA TAGATGTAGT GAGAAGAGGG 
GAAGAGCCCA CAGCTAGGAC AGAGGAAACC AGGCAAGCCA GAAGCCTGGG TGATAGACTC 
CAGGATGGAA TGACCACCTC TAGAGAGCAA GACCTGGAGG TCCCACTGGA TGACATCCCT 
GCTCCTGCCG CTCCATTTGA TCATCGGATG GTAATGGCCA AACATGCTTC AGTGGACAAT 
CTGTACACCG TGAGCAAATC TGAGATCCTA GGAGGGGGGC GTTTTGGTCA GGTACATAAG 
TGTGAAGAGA AAGCAACAGG CCTGAAGCTG GCTGCTAAAA TCATTAAGAC TAGAGGTGCA 
AAGGACAAGG AGGATGTGAA GAACGAGATC AGCGTCATGA ACCAGCTGGA CCACGTGAAC 
CTCATTCAGC TCTATGATGC CTTTGAGTCC AAGCATGACA TCATCCTTGT CATGGAGTAT 
GTGGAGGGTG GGGAGCTCTT TGACCGCATC ATTGATGAAA ACTGCAACTT GACAGAGCTG 
GACACCATCC TGTTCATGAA GCAGATCTGT GAGGGGATAC GGTACATGCA CCAGATGTAC 
ATCCTCCACT TGGATCTCAA GCCTGAGAAC ATCCTGTGTG TGAATCGGGA TGCTAAGCAA 
ATAAAAATTA TTGATTTTGG ATTGGCCAGA AGATACAAGC CCAGAGAGAA GCTGAAGGTG 
AACTTTGGAA CCCCAGAATT TCTTGCCCCT GAAGTTGTGA ACTATGATTT CGTTTCCTTT 
TCTACAGATA TGTGGAGTGT GGGCGTCATC ACATACATGC TACTCAGTGG CTTGTCCCCC 
TTTTTGGGAG ACAATGATGC CGAGACACTG ACCAACATCC TGGCGTGCCG GTGGGATTTA 
GAAGATGAGG AATTTCAGGA TATCTCGGAG GAGGCAAAGG AGTTCATCTC CAAGCTCCTG 
ATTAAGGAGA AGAGTTGGAG AATAAGTGCC AGCGAAGCTC TCAAGCACCC ATGGTTGTCA 
GACCACAAGC TCCACTCCAG GCTGAGTGCA CAGAAGAACT GCAACTCTGG TGTTCTGAAC 
CTCACGACCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355809.1
CDS	299..2173
Translation	MLKVKRLEEISSCHSSNPLEKVAFFQCMEEVEKVKCFLEENSGDLDLQSGDNEAEENVWSNRALDERIIVKGGRTSALTEKTDQNDQFSSEKEKITKLNKKNSEKTSALEVQRSCVICHVERQDKIEFVQRNSTELRENAGAKDAPCIESLEKQESNNLRDKQDTEDNNGGENGISSKDDSRGVEAENRASAAASGAVLDKHSTESQHTEDMNGDPSHERCISNIKTTSTPGILGNSASGSTFQNAEQTESTGSSKRRVAEECFLSEDNKKSRIDVVRRGEEPTARTEETRQARSLGDRLQDGMTTSREQDLEVPLDDIPAPAAPFDHRMVMAKHASVDNLYTVSKSEILGGGRFGQVHKCEEKATGLKLAAKIIKTRGAKDKEDVKNEISVMNQLDHVNLIQLYDAFESKHDIILVMEYVEGGELFDRIIDENCNLTELDTILFMKQICEGIRYMHQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSFSTDMWSVGVITYMLLSGLSPFLGDNDAETLTNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRISASEALKHPWLSDHKLHSRLSAQKNCNSGVLNLTTK

Target ORF information:

RefSeq Version	NM_001368880.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase family, member 4 (Mylk4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368880.1

ORF Insert Sequence:

ATGTTGAAGG TGAAGAGGCT GGAAGAAATC AGCTCGTGCC ACAGCAGCAA CCCGCTGGAG 
AAGGTGGCGT TTTTCCAGTG CATGGAGGAG GTGGAGAAGG TGAAATGCTT CCTGGAGGAG 
AATTCGGGGG ATCTGGACCT GCAGTCTGGA GACAATGAGG CTGAGGAAAA TGTATGGTCC 
AACCGTGCAC TAGATGAAAG GATAATTGTC AAAGGAGGAA GGACATCAGC CCTCACAGAG 
AAAACTGACC AGAATGACCA ATTCTCTTCC GAGAAAGAAA AGATTACCAA ACTTAACAAG 
AAAAATTCAG AGAAGACCAG TGCATTAGAA GTGCAGAGGA GTTGTGTCAT ATGTCACGTG 
GAAAGACAAG ACAAGATAGA ATTTGTTCAG AGGAATTCCA CAGAGCTAAG AGAGAATGCA 
GGTGCAAAAG ATGCTCCATG CATAGAAAGT CTGGAGAAAC AAGAAAGCAA CAACCTGAGA 
GATAAACAAG ACACTGAAGA TAACAATGGA GGAGAAAATG GGATTTCTTC TAAGGACGAT 
TCCAGAGGAG TAGAAGCTGA GAACAGAGCT TCAGCAGCTG CAAGTGGAGC AGTGCTAGAC 
AAACACTCGA CTGAAAGCCA GCACACTGAG GACATGAATG GTGACCCGTC CCATGAAAGG 
TGCATCAGTA ATATAAAGAC CACTTCTACA CCAGGAATCC TTGGAAATTC AGCATCAGGA 
TCCACTTTCC AGAATGCTGA ACAGACAGAG TCTACTGGCA GCAGTAAGCG CAGGGTGGCA 
GAGGAGTGCT TCCTCAGTGA GGACAACAAA AAGAGCAGAA TAGATGTAGT GAGAAGAGGG 
GAAGAGCCCA CAGCTAGGAC AGAGGAAACC AGGCAAGCCA GAAGCCTGGG TGATAGACTC 
CAGGATGGAA TGACCACCTC TAGAGAGCAA GACCTGGAGG TCCCACTGGA TGACATCCCT 
GCTCCTGCCG CTCCATTTGA TCATCGGATG GTAATGGCCA AACATGCTTC AGTGGACAAT 
CTGTACACCG TGAGCAAATC TGAGATCCTA GGAGGGGGGC GTTTTGGTCA GGTACATAAG 
TGTGAAGAGA AAGCAACAGG CCTGAAGCTG GCTGCTAAAA TCATTAAGAC TAGAGGTGCA 
AAGGACAAGG AGGATGTGAA GAACGAGATC AGCGTCATGA ACCAGCTGGA CCACGTGAAC 
CTCATTCAGC TCTATGATGC CTTTGAGTCC AAGCATGACA TCATCCTTGT CATGGAGTAT 
GTGGAGGGTG GGGAGCTCTT TGACCGCATC ATTGATGAAA ACTGCAACTT GACAGAGCTG 
GACACCATCC TGTTCATGAA GCAGATCTGT GAGGGGATAC GGTACATGCA CCAGATGTAC 
ATCCTCCACT TGGATCTCAA GCCTGAGAAC ATCCTGTGTG TGAATCGGGA TGCTAAGCAA 
ATAAAAATTA TTGATTTTGG ATTGGCCAGA AGATACAAGC CCAGAGAGAA GCTGAAGGTG 
AACTTTGGAA CCCCAGAATT TCTTGCCCCT GAAGTTGTGA ACTATGATTT CGTTTCCTTT 
TCTACAGATA TGTGGAGTGT GGGCGTCATC ACATACATGC TACTCAGTGG CTTGTCCCCC 
TTTTTGGGAG ACAATGATGC CGAGACACTG ACCAACATCC TGGCGTGCCG GTGGGATTTA 
GAAGATGAGG AATTTCAGGA TATCTCGGAG GAGGCAAAGG AGTTCATCTC CAAGCTCCTG 
ATTAAGGAGA AGAGTTGGAG AATAAGTGCC AGCGAAGCTC TCAAGCACCC ATGGTTGTCA 
GACCACAAGC TCCACTCCAG GCTGAGTGCA CAGAAGAACT GCAACTCTGG TGTTCTGAAC 
CTCACGACCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk4 ( XM_006516667.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516730.1 Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X2, mRNA.

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CloneID	OMu48386
Clone ID Related Accession (Same CDS sequence)	XM_006516667.2 , XM_006516667.3
Accession Version	XM_006516667.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCT ACAGATGTCA AGAGAAGGGA GTGCTGAGCA GTCATGATTG TGTCCTCATT 
TACAAATCTG AGACAGAGGG AGATGAAAGC CCCACTCCTG ACAGCAGCCT GATCAGGCTC 
ACCACCAGTA TGTTGAAGGT GAAGAGGCTG GAAGAAATCA GCTCGTGCCA CAGCAGCAAC 
CCGCTGGAGA AGGTGGCGTT TTTCCAGTGC ATGGAGGAGG TGGAGAAGGT GAAATGCTTC 
CTGGAGGAGA ATTCGGGGGA TCTGGACCTG CAGTCTGGAG ACAATGAGGC TGAGGAAAAT 
GTATGGTCCA ACCGTGCACT AGATGAAAGG ATAATTGTCA AAGGAGGAAG GACATCAGCC 
CTCACAGAGA AAACTGACCA GAATGACCAA TTCTCTTCCG AGAAAGAAAA GATTACCAAA 
CTTAACAAGA AAAATTCAGA GAAGACCAGT GCATTAGAAG TGCAGAGGAG TTGTGTCATA 
TGTCACGTGG AAAGACAAGA CAAGATAGAA TTTGTTCAGA GGAATTCCAC AGAGCTAAGA 
GAGAATGCAG GTGCAAAAGA TGCTCCATGC ATAGAAAGTC TGGAGAAACA AGAAAGCAAC 
AACCTGAGAG ATAAACAAGA CACTGAAGAT AACAATGGAG GAGAAAATGG GATTTCTTCT 
AAGGACGATT CCAGAGGAGT AGAAGCTGAG AACAGAGCTT CAGCAGCTGC AAGTGGAGCA 
GTGCTAGACA AACACTCGAC TGAAAGCCAG CACACTGAGG ACATGAATGG TGACCCGTCC 
CATGAAAGGT GCATCAGTAA TATAAAGACC ACTTCTACAC CAGGAATCCT TGGAAATTCA 
GCATCAGGAT CCACTTTCCA GAATGCTGAA CAGACAGAGT CTACTGGCAG CAGTAAGCGC 
AGGGTGGCAG AGGAGTGCTT CCTCAGTGAG GACAACAAAA AGAGCAGAAT AGATGTAGTG 
AGAAGAGGGG AAGAGCCCAC AGCTAGGACA GAGGAAACCA GGCAAGCCAG AAGCCTGGGT 
GATAGACTCC AGGATGGAAT GACCACCTCT AGAGAGCAAG ACCTGGAGGT CCCACTGGAT 
GACATCCCTG CTCCTGCCGC TCCATTTGAT CATCGGATGG TAATGGCCAA ACATGCTTCA 
GTGGACAATC TGTACACCGT GAGCAAATCT GAGATCCTAG GAGGGGGGCG TTTTGGTCAG 
GTACATAAGT GTGAAGAGAA AGCAACAGGC CTGAAGCTGG CTGCTAAAAT CATTAAGACT 
AGAGGTGCAA AGGACAAGGA GGATGTGAAG AACGAGATCA GCGTCATGAA CCAGCTGGAC 
CACGTGAACC TCATTCAGCT CTATGATGCC TTTGAGTCCA AGCATGACAT CATCCTTGTC 
ATGGAGTATG TGGAGGGTGG GGAGCTCTTT GACCGCATCA TTGATGAAAA CTGCAACTTG 
ACAGAGCTGG ACACCATCCT GTTCATGAAG CAGATCTGTG AGGGGATACG GTACATGCAC 
CAGATGTACA TCCTCCACTT GGATCTCAAG CCTGAGAACA TCCTGTGTGT GAATCGGGAT 
GCTAAGCAAA TAAAAATTAT TGATTTTGGA TTGGCCAGAA GATACAAGCC CAGAGAGAAG 
CTGAAGGTGA ACTTTGGAAC CCCAGAATTT CTTGCCCCTG AAGTTGTGAA CTATGATTTC 
GTTTCCTTTT CTACAGATAT GTGGAGTGTG GGCGTCATCA CATACATGCT ACTCAGTGGC 
TTGTCCCCCT TTTTGGGAGA CAATGATGCC GAGACACTGA CCAACATCCT GGCGTGCCGG 
TGGGATTTAG AAGATGAGGA ATTTCAGGAT ATCTCGGAGG AGGCAAAGGA GTTCATCTCC 
AAGCTCCTGA TTAAGGAGAA GAGTTGGAGA ATAAGTGCCA GCGAAGCTCT CAAGCACCCA 
TGGTTGTCAG ACCACAAGCT CCACTCCAGG CTGAGTGCAC AGAAGAACTG CAACTCTGGT 
GTTCTGAACC TCACGACCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516730.1
CDS	231..2234
Translation	MSSYRCQEKGVLSSHDCVLIYKSETEGDESPTPDSSLIRLTTSMLKVKRLEEISSCHSSNPLEKVAFFQCMEEVEKVKCFLEENSGDLDLQSGDNEAEENVWSNRALDERIIVKGGRTSALTEKTDQNDQFSSEKEKITKLNKKNSEKTSALEVQRSCVICHVERQDKIEFVQRNSTELRENAGAKDAPCIESLEKQESNNLRDKQDTEDNNGGENGISSKDDSRGVEAENRASAAASGAVLDKHSTESQHTEDMNGDPSHERCISNIKTTSTPGILGNSASGSTFQNAEQTESTGSSKRRVAEECFLSEDNKKSRIDVVRRGEEPTARTEETRQARSLGDRLQDGMTTSREQDLEVPLDDIPAPAAPFDHRMVMAKHASVDNLYTVSKSEILGGGRFGQVHKCEEKATGLKLAAKIIKTRGAKDKEDVKNEISVMNQLDHVNLIQLYDAFESKHDIILVMEYVEGGELFDRIIDENCNLTELDTILFMKQICEGIRYMHQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSFSTDMWSVGVITYMLLSGLSPFLGDNDAETLTNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRISASEALKHPWLSDHKLHSRLSAQKNCNSGVLNLTTK

Target ORF information:

RefSeq Version	XM_006516667.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516667.3

ORF Insert Sequence:

ATGTCCTCCT ACAGATGTCA AGAGAAGGGA GTGCTGAGCA GTCATGATTG TGTCCTCATT 
TACAAATCTG AGACAGAGGG AGATGAAAGC CCCACTCCTG ACAGCAGCCT GATCAGGCTC 
ACCACCAGTA TGTTGAAGGT GAAGAGGCTG GAAGAAATCA GCTCGTGCCA CAGCAGCAAC 
CCGCTGGAGA AGGTGGCGTT TTTCCAGTGC ATGGAGGAGG TGGAGAAGGT GAAATGCTTC 
CTGGAGGAGA ATTCGGGGGA TCTGGACCTG CAGTCTGGAG ACAATGAGGC TGAGGAAAAT 
GTATGGTCCA ACCGTGCACT AGATGAAAGG ATAATTGTCA AAGGAGGAAG GACATCAGCC 
CTCACAGAGA AAACTGACCA GAATGACCAA TTCTCTTCCG AGAAAGAAAA GATTACCAAA 
CTTAACAAGA AAAATTCAGA GAAGACCAGT GCATTAGAAG TGCAGAGGAG TTGTGTCATA 
TGTCACGTGG AAAGACAAGA CAAGATAGAA TTTGTTCAGA GGAATTCCAC AGAGCTAAGA 
GAGAATGCAG GTGCAAAAGA TGCTCCATGC ATAGAAAGTC TGGAGAAACA AGAAAGCAAC 
AACCTGAGAG ATAAACAAGA CACTGAAGAT AACAATGGAG GAGAAAATGG GATTTCTTCT 
AAGGACGATT CCAGAGGAGT AGAAGCTGAG AACAGAGCTT CAGCAGCTGC AAGTGGAGCA 
GTGCTAGACA AACACTCGAC TGAAAGCCAG CACACTGAGG ACATGAATGG TGACCCGTCC 
CATGAAAGGT GCATCAGTAA TATAAAGACC ACTTCTACAC CAGGAATCCT TGGAAATTCA 
GCATCAGGAT CCACTTTCCA GAATGCTGAA CAGACAGAGT CTACTGGCAG CAGTAAGCGC 
AGGGTGGCAG AGGAGTGCTT CCTCAGTGAG GACAACAAAA AGAGCAGAAT AGATGTAGTG 
AGAAGAGGGG AAGAGCCCAC AGCTAGGACA GAGGAAACCA GGCAAGCCAG AAGCCTGGGT 
GATAGACTCC AGGATGGAAT GACCACCTCT AGAGAGCAAG ACCTGGAGGT CCCACTGGAT 
GACATCCCTG CTCCTGCCGC TCCATTTGAT CATCGGATGG TAATGGCCAA ACATGCTTCA 
GTGGACAATC TGTACACCGT GAGCAAATCT GAGATCCTAG GAGGGGGGCG TTTTGGTCAG 
GTACATAAGT GTGAAGAGAA AGCAACAGGC CTGAAGCTGG CTGCTAAAAT CATTAAGACT 
AGAGGTGCAA AGGACAAGGA GGATGTGAAG AACGAGATCA GCGTCATGAA CCAGCTGGAC 
CACGTGAACC TCATTCAGCT CTATGATGCC TTTGAGTCCA AGCATGACAT CATCCTTGTC 
ATGGAGTATG TGGAGGGTGG GGAGCTCTTT GACCGCATCA TTGATGAAAA CTGCAACTTG 
ACAGAGCTGG ACACCATCCT GTTCATGAAG CAGATCTGTG AGGGGATACG GTACATGCAC 
CAGATGTACA TCCTCCACTT GGATCTCAAG CCTGAGAACA TCCTGTGTGT GAATCGGGAT 
GCTAAGCAAA TAAAAATTAT TGATTTTGGA TTGGCCAGAA GATACAAGCC CAGAGAGAAG 
CTGAAGGTGA ACTTTGGAAC CCCAGAATTT CTTGCCCCTG AAGTTGTGAA CTATGATTTC 
GTTTCCTTTT CTACAGATAT GTGGAGTGTG GGCGTCATCA CATACATGCT ACTCAGTGGC 
TTGTCCCCCT TTTTGGGAGA CAATGATGCC GAGACACTGA CCAACATCCT GGCGTGCCGG 
TGGGATTTAG AAGATGAGGA ATTTCAGGAT ATCTCGGAGG AGGCAAAGGA GTTCATCTCC 
AAGCTCCTGA TTAAGGAGAA GAGTTGGAGA ATAAGTGCCA GCGAAGCTCT CAAGCACCCA 
TGGTTGTCAG ACCACAAGCT CCACTCCAGG CTGAGTGCAC AGAAGAACTG CAACTCTGGT 
GTTCTGAACC TCACGACCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk4 ( XM_006516668.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516731.1 Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu48387
Clone ID Related Accession (Same CDS sequence)	XM_006516668.3 , XM_006516668.4
Accession Version	XM_006516668.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase family member 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516668.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCACTC CTCAGAGTAG AGCAGGGAGC ACAGGGAACT TCAAAAGTGG CTGTGCTAGA 
CCAGGGCAGG GAAGGTGCCC ACAAAGCCAG GGACTTCACA GCCTCTCCCA GAGAATGGAC 
TCCACGCTTC GAAGAAAAGA CCTCTGGATT GTGGGTAGCG TGTGCCTTCT GTCGTCTTCG 
GTCTTCTGGC GCCTGTTTCG GTCAGTGGCG GCATGGGGAG GCCCCAAGCA GGAACCTGCA 
CTTGAGAATG CACAGGCTGA GGAAAATGTA TGGTCCAACC GTGCACTAGA TGAAAGGATA 
ATTGTCAAAG GAGGAAGGAC ATCAGCCCTC ACAGAGAAAA CTGACCAGAA TGACCAATTC 
TCTTCCGAGA AAGAAAAGAT TACCAAACTT AACAAGAAAA ATTCAGAGAA GACCAGTGCA 
TTAGAAGTGC AGAGGAGTTG TGTCATATGT CACGTGGAAA GACAAGACAA GATAGAATTT 
GTTCAGAGGA ATTCCACAGA GCTAAGAGAG AATGCAGGTG CAAAAGATGC TCCATGCATA 
GAAAGTCTGG AGAAACAAGA AAGCAACAAC CTGAGAGATA AACAAGACAC TGAAGATAAC 
AATGGAGGAG AAAATGGGAT TTCTTCTAAG GACGATTCCA GAGGAGTAGA AGCTGAGAAC 
AGAGCTTCAG CAGCTGCAAG TGGAGCAGTG CTAGACAAAC ACTCGACTGA AAGCCAGCAC 
ACTGAGGACA TGAATGGTGA CCCGTCCCAT GAAAGGTGCA TCAGTAATAT AAAGACCACT 
TCTACACCAG GAATCCTTGG AAATTCAGCA TCAGGATCCA CTTTCCAGAA TGCTGAACAG 
ACAGAGTCTA CTGGCAGCAG TAAGCGCAGG GTGGCAGAGG AGTGCTTCCT CAGTGAGGAC 
AACAAAAAGA GCAGAATAGA TGTAGTGAGA AGAGGGGAAG AGCCCACAGC TAGGACAGAG 
GAAACCAGGC AAGCCAGAAG CCTGGGTGAT AGACTCCAGG ATGGAATGAC CACCTCTAGA 
GAGCAAGACC TGGAGGTCCC ACTGGATGAC ATCCCTGCTC CTGCCGCTCC ATTTGATCAT 
CGGATGGTAA TGGCCAAACA TGCTTCAGTG GACAATCTGT ACACCGTGAG CAAATCTGAG 
ATCCTAGGAG GGGGGCGTTT TGGTCAGGTA CATAAGTGTG AAGAGAAAGC AACAGGCCTG 
AAGCTGGCTG CTAAAATCAT TAAGACTAGA GGTGCAAAGG ACAAGGAGGA TGTGAAGAAC 
GAGATCAGCG TCATGAACCA GCTGGACCAC GTGAACCTCA TTCAGCTCTA TGATGCCTTT 
GAGTCCAAGC ATGACATCAT CCTTGTCATG GAGTATGTGG AGGGTGGGGA GCTCTTTGAC 
CGCATCATTG ATGAAAACTG CAACTTGACA GAGCTGGACA CCATCCTGTT CATGAAGCAG 
ATCTGTGAGG GGATACGGTA CATGCACCAG ATGTACATCC TCCACTTGGA TCTCAAGCCT 
GAGAACATCC TGTGTGTGAA TCGGGATGCT AAGCAAATAA AAATTATTGA TTTTGGATTG 
GCCAGAAGAT ACAAGCCCAG AGAGAAGCTG AAGGTGAACT TTGGAACCCC AGAATTTCTT 
GCCCCTGAAG TTGTGAACTA TGATTTCGTT TCCTTTTCTA CAGATATGTG GAGTGTGGGC 
GTCATCACAT ACATGCTACT CAGTGGCTTG TCCCCCTTTT TGGGAGACAA TGATGCCGAG 
ACACTGACCA ACATCCTGGC GTGCCGGTGG GATTTAGAAG ATGAGGAATT TCAGGATATC 
TCGGAGGAGG CAAAGGAGTT CATCTCCAAG CTCCTGATTA AGGAGAAGAG TTGGAGAATA 
AGTGCCAGCG AAGCTCTCAA GCACCCATGG TTGTCAGACC ACAAGCTCCA CTCCAGGCTG 
AGTGCACAGA AGAACTGCAA CTCTGGTGTT CTGAACCTCA CGACCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516731.1
CDS	227..2197
Translation	MPTPQSRAGSTGNFKSGCARPGQGRCPQSQGLHSLSQRMDSTLRRKDLWIVGSVCLLSSSVFWRLFRSVAAWGGPKQEPALENAQAEENVWSNRALDERIIVKGGRTSALTEKTDQNDQFSSEKEKITKLNKKNSEKTSALEVQRSCVICHVERQDKIEFVQRNSTELRENAGAKDAPCIESLEKQESNNLRDKQDTEDNNGGENGISSKDDSRGVEAENRASAAASGAVLDKHSTESQHTEDMNGDPSHERCISNIKTTSTPGILGNSASGSTFQNAEQTESTGSSKRRVAEECFLSEDNKKSRIDVVRRGEEPTARTEETRQARSLGDRLQDGMTTSREQDLEVPLDDIPAPAAPFDHRMVMAKHASVDNLYTVSKSEILGGGRFGQVHKCEEKATGLKLAAKIIKTRGAKDKEDVKNEISVMNQLDHVNLIQLYDAFESKHDIILVMEYVEGGELFDRIIDENCNLTELDTILFMKQICEGIRYMHQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSFSTDMWSVGVITYMLLSGLSPFLGDNDAETLTNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRISASEALKHPWLSDHKLHSRLSAQKNCNSGVLNLTTK

Target ORF information:

RefSeq Version	XM_006516668.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase family, member 4 (Mylk4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516668.4

ORF Insert Sequence:

ATGCCCACTC CTCAGAGTAG AGCAGGGAGC ACAGGGAACT TCAAAAGTGG CTGTGCTAGA 
CCAGGGCAGG GAAGGTGCCC ACAAAGCCAG GGACTTCACA GCCTCTCCCA GAGAATGGAC 
TCCACGCTTC GAAGAAAAGA CCTCTGGATT GTGGGTAGCG TGTGCCTTCT GTCGTCTTCG 
GTCTTCTGGC GCCTGTTTCG GTCAGTGGCG GCATGGGGAG GCCCCAAGCA GGAACCTGCA 
CTTGAGAATG CACAGGCTGA GGAAAATGTA TGGTCCAACC GTGCACTAGA TGAAAGGATA 
ATTGTCAAAG GAGGAAGGAC ATCAGCCCTC ACAGAGAAAA CTGACCAGAA TGACCAATTC 
TCTTCCGAGA AAGAAAAGAT TACCAAACTT AACAAGAAAA ATTCAGAGAA GACCAGTGCA 
TTAGAAGTGC AGAGGAGTTG TGTCATATGT CACGTGGAAA GACAAGACAA GATAGAATTT 
GTTCAGAGGA ATTCCACAGA GCTAAGAGAG AATGCAGGTG CAAAAGATGC TCCATGCATA 
GAAAGTCTGG AGAAACAAGA AAGCAACAAC CTGAGAGATA AACAAGACAC TGAAGATAAC 
AATGGAGGAG AAAATGGGAT TTCTTCTAAG GACGATTCCA GAGGAGTAGA AGCTGAGAAC 
AGAGCTTCAG CAGCTGCAAG TGGAGCAGTG CTAGACAAAC ACTCGACTGA AAGCCAGCAC 
ACTGAGGACA TGAATGGTGA CCCGTCCCAT GAAAGGTGCA TCAGTAATAT AAAGACCACT 
TCTACACCAG GAATCCTTGG AAATTCAGCA TCAGGATCCA CTTTCCAGAA TGCTGAACAG 
ACAGAGTCTA CTGGCAGCAG TAAGCGCAGG GTGGCAGAGG AGTGCTTCCT CAGTGAGGAC 
AACAAAAAGA GCAGAATAGA TGTAGTGAGA AGAGGGGAAG AGCCCACAGC TAGGACAGAG 
GAAACCAGGC AAGCCAGAAG CCTGGGTGAT AGACTCCAGG ATGGAATGAC CACCTCTAGA 
GAGCAAGACC TGGAGGTCCC ACTGGATGAC ATCCCTGCTC CTGCCGCTCC ATTTGATCAT 
CGGATGGTAA TGGCCAAACA TGCTTCAGTG GACAATCTGT ACACCGTGAG CAAATCTGAG 
ATCCTAGGAG GGGGGCGTTT TGGTCAGGTA CATAAGTGTG AAGAGAAAGC AACAGGCCTG 
AAGCTGGCTG CTAAAATCAT TAAGACTAGA GGTGCAAAGG ACAAGGAGGA TGTGAAGAAC 
GAGATCAGCG TCATGAACCA GCTGGACCAC GTGAACCTCA TTCAGCTCTA TGATGCCTTT 
GAGTCCAAGC ATGACATCAT CCTTGTCATG GAGTATGTGG AGGGTGGGGA GCTCTTTGAC 
CGCATCATTG ATGAAAACTG CAACTTGACA GAGCTGGACA CCATCCTGTT CATGAAGCAG 
ATCTGTGAGG GGATACGGTA CATGCACCAG ATGTACATCC TCCACTTGGA TCTCAAGCCT 
GAGAACATCC TGTGTGTGAA TCGGGATGCT AAGCAAATAA AAATTATTGA TTTTGGATTG 
GCCAGAAGAT ACAAGCCCAG AGAGAAGCTG AAGGTGAACT TTGGAACCCC AGAATTTCTT 
GCCCCTGAAG TTGTGAACTA TGATTTCGTT TCCTTTTCTA CAGATATGTG GAGTGTGGGC 
GTCATCACAT ACATGCTACT CAGTGGCTTG TCCCCCTTTT TGGGAGACAA TGATGCCGAG 
ACACTGACCA ACATCCTGGC GTGCCGGTGG GATTTAGAAG ATGAGGAATT TCAGGATATC 
TCGGAGGAGG CAAAGGAGTT CATCTCCAAG CTCCTGATTA AGGAGAAGAG TTGGAGAATA 
AGTGCCAGCG AAGCTCTCAA GCACCCATGG TTGTCAGACC ACAAGCTCCA CTCCAGGCTG 
AGTGCACAGA AGAACTGCAA CTCTGGTGTT CTGAACCTCA CGACCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiac myosin light chain is phosphorylated by Ca2+/calmodulin-dependent and -independent kinase activities.
Proceedings of the National Academy of Sciences of the United States of America113(27)E3824-33(2016 07)
Chang AN,Mahajan P,Knapp S,Barton H,Sweeney HL,Kamm KE,Stull JT

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Mylk4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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