Piezo1 cDNA ORF clone, Mus musculus(house mouse)

The following Piezo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Piezo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu41426 XM_006530959.3
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu73794 XM_011248372.1
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu73789 XM_006530953.2
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu18271 NM_001037298.1
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1868.30
$2669.00
OMu41422 NM_001357349.1
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu41422 XM_006530955.2
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu73790 XM_006530954.2
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu73791 XM_006530956.2
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu73792 XM_006530957.3
Latest version!
Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu41426
    Clone ID Related Accession (Same CDS sequence) XM_006530959.3
    Accession Version XM_006530959.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5454bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530959.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGGCTCAC AGGATGCAGT GAGCGAGGCC CCTCTGCTTG AGCATCAGGA GGAAGAGGAA 
    GTCTTCAGGG AAGATGGGCA GAGCATGGAT GGGCCCCACC AGGCCACACA GGTCCCTGAG
    GGTACGGCCA GCAAGTGGGG CCTGGTGGCT GACCGGCTGC TGGACCTAGC GGCCAGCTTC
    TCGGCTGTCC TCACCCGAAT CCAGGTGTTC GTTCGGCGCT TGCTAGAACT TCACGTCTTC
    AAGCTGGTGG CCCTCTACAC TGTCTGGGTG GCCCTGAAAG AAGTGTCTGT GATGAACCTG
    CTGCTGGTGG TGCTATGGGC CTTCGCCTTG CCCTATCCGC GCTTCCGGCC CATGGCTTCC
    TGCCTGTCCA CCGTGTGGAC CTGTATCATC ATTGTATGCA AGATGCTCTA TCAGCTCAAG
    ATTGTCAACC CGCATGAGTA CTCCAGCAAC TGCACTGAGC CCTTCCCCAA CAATACCAAC
    TTGCAGCCTT TGGAGATCAA CCAGTCTTTG CTGTACCGTG GCCCTGTTGA CCCTGCCAAC
    TGGTTTGGGG TGCGGAAGGG TTACCCCAAC TTGGGCTATA TCCAGAACCA CCTGCAGATC
    CTTCTGTTGC TGGTGTTTGA GGCCGTGGTG TACCGGCGCC AAGAGCACTA CCGCCGGCAG
    CACCAGCAGG CCCCTCTGCC CGCCCAGGCT GTGTGCGCAG ATGGCACCCG CCAGAGGTTG
    GACCAGGACC TACTTAGCTG CCTCAAGTAT TTCATCAACT TCTTCTTCTA CAAATTCGGG
    CTGGAGATCT GCTTCTTGAT GGCCGTGAAT GTGATTGGGC AGCGTATGAA CTTCATGGTG
    ATCTTACACG GTTGCTGGTT GGTGGCCATC CTTACACGCC GGCGCCGTGA GGCCATCGCC
    CGCCTCTGGC CTAACTACTG TCTGTTCCTC ACGCTGTTCC TGCTGTACCA GTACCTGCTG
    TGTTTGGGCA TGCCCCCTGC ACTGTGCATT GACTATCCAT GGCGCTGGAG CAAGGCCATC
    CCCATGAATT CCGCCCTCAT CAAGTGGCTG TACCTACCTG ACTTCTTCAG AGCCCCCAAC
    TCCACCAACC TTATCAGTGA CTTCCTCCTG CTGCTTTGCG CCTCCCAGCA GTGGCAGGTC
    TTCTCAGCGG AGCGAACGGA GGAGTGGCAA CGCATGGCGG GCATCAACAC TGACCACCTG
    GAGCCCCTGC GTGGGGAGCC CAACCCTATA CCCAACTTCA TCCACTGCAG GTCCTATCTG
    GATATGCTGA AGGTGGCCGT CTTCCGCTAC CTGTTCTGGC TGGTGCTCGT TGTGGTGTTT
    GTTGCGGGGG CCACCCGCAT AAGCATCTTC GGGCTGGGGT ACCTGCTAGC CTGCTTCTAC
    CTGCTGCTGT TTGGCACTAC CCTGCTGCAG AAGGACACGC GAGCCCAGCT CGTGCTGTGG
    GACTGCCTCA TCCTCTATAA TGTCACTGTC ATCATCTCTA AGAATATGCT GTCGCTCCTG
    TCCTGTGTCT TCGTGGAGCA AATGCAGAGC AACTTCTGCT GGGTCATCCA GCTCTTCAGC
    CTCGTGTGCA CAGTCAAAGG CTACTATGAT CCCAAAGAGA TGATGACCAG GGACCGGGAC
    TGCCTGCTGC CTGTGGAGGA GGCCGGGATC ATCTGGGACA GTATCTGCTT CTTCTTCCTG
    CTCTTGCAAC GGCGCATCTT TCTCAGCCAC TACTTCCTGC ATGTCAGCGC TGACCTGAAA
    GCCACAGCCC TGCAGGCATC CAGGGGCTTT GCCCTCTACA ATGCAGCCAA CCTGAAGAGC
    ATCAACTTCC ATCGCCAGAT TGAGGAGAAG TCCCTGGCCC AGCTGAAAAG ACAGATGAAG
    CGCATCCGTG CCAAACAGGA GAAGTACAGG CAGAGCCAGG CAAGTCGTGG CCAACTCCAG
    TCCAAAGACC CTCAGGATCC CAGCCAGGAG CCAGGGCCTG ACAGCCCAGG GGGCTCCTCC
    CCGCCACGGA GACAGTGGTG GCGCCCCTGG CTGGACCACG CCACAGTCAT CCACTCTGGC
    GACTACTTCC TGTTTGAGTC AGATAGCGAG GAGGAAGAGG AGGCCCTACC TGAGGACCCC
    AGGCCTGCAG CTCAGAGTGC CTTCCAGATG GCATACCAGG CATGGGTAAC CAATGCCCAG
    ACAGTGCTGA GGCAGCGTCG GGAGCGGGCA CGGCAGGAGC GGGCAGAGCA GCTGGCTTCT
    GGAGGTGACT TGAACCCAGA TGTGGAACCA GTAGATGTCC CAGAAGATGA GATGGCAGGC
    CGTAGCCACA TGATGCAGCG TGTGCTAAGC ACCATGCAGT TCCTGTGGGT GCTGGGCCAG
    GCCACGGTAG ACGGGCTGAC GCGCTGGCTG CGTGCATTCA CGAAGCACCA CCGCACCATG
    AGCGATGTGC TGTGCGCAGA GCGCTACCTG CTCACCCAGG AGCTTCTTCG GGTTGGAGAG
    GTACGCCGAG GTGTGCTGGA CCAGCTTTAT GTGGGTGAAG ATGAGGCCAC ATTGTCAGGT
    CCCATGGAGA CCCGGGATGG ACCCAGCACA GCCTCAAGTG GGCTGGGAGC CGAAGAGCCT
    TTGAGTAGCA TGACAGACGA CACCAGCAGC CCCCTGAGCA CAGGCTATAA CACCCGCAGT
    GGCAGTGAGG AGATTGTCAC CGACGCTGGG GACCTCCAGG CTGGGACCTC CCTGCACGGC
    TCCCAAGAGC TTTTAGCCAA TGCTCGTACC CGGATGCGCA CGGCCAGCGA GCTGCTACTG
    GATAGGCGCC TGCATATCCC TGAGCTGGAG GAGGCCGAGC GGTTTGAGGC ACAGCAGGGC
    CGGACTCTGC GGCTGCTCAG GGCTGGGTAC CAGTGCGTGG CGGCACACTC GGAGCTGCTC
    TGTTACTTCA TCATCATCCT TAACCACATG GTGACAGCCT CGGCTGCCTC CCTGGTGCTG
    CCCGTGCTTG TGTTCCTGTG GGCCATGCTG ACCATCCCGA GGCCTAGCAA GCGCTTTTGG
    ATGACAGCTA TCGTCTTCAC TGAGGTCATG GTGGTCACCA AATACCTGTT CCAGTTCGGC
    TTCTTCCCCT GGAACAGCTA CGTTGTGCTG CGGCGCTATG AGAACAAGCC CTACTTCCCT
    CCGCGAATCC TGGGCCTTGA GAAAACGGAC AGCTACATCA AGTATGACCT GGTGCAGCTC
    ATGGCCCTCT TCTTCCACCG CTCGCAGCTA CTGTGTTATG GCCTCTGGGA CCATGAGGAG
    GATCGCTATC CCAAGGACCA TTGCAGGAGT AGTGTGAAGG ACCGGGAGGC CAAGGAAGAG
    CCAGAAGCTA AGCTGGAATC GCAGTCTGAG ACAGGCACTG GGCATCCCAA GGAGCCAGTG
    TTGGCCGGTA CTCCCAGGGA CCACATCCAA GGGAAAGGAA GTATTAGATC CAAGGATGTT
    ATCCAAGATC CCCCAGAGGA CCTTAAGCCC CGGCACACGA GGCACATCAG CATACGCTTC
    AGGAGGCGCA AGGAGACTCC AGGACCCAAA GGAACAGCAG TCATGGAGAC TGAGCACGAG
    GAGGGAGAAG GAAAAGAAAC TACAGAGAGA AAGAGGCCGC GTCACACTCA AGAAAAATCG
    AAGTTTCGGG AGAGAATGAA GGCAGCTGGG CGCCGGCTGC AGAGCTTCTG TGTGTCACTG
    GCCCAGAGCT TCTACCAACC CTTGCAGCGT TTCTTCCATG ACATTCTGCA CACAAAGTAC
    CGGGCGGCCA CCGACGTCTA CGCCCTCATG TTCCTGGCCG ATATTGTCGA CATCATCATC
    ATCATCTTTG GTTTTTGGGC TTTTGGGAAG CACTCTGCAG CCACAGACAT TGCATCCTCG
    CTGTCAGATG ACCAGGTGCC ACAGGCCTTC CTGTTCATGC TGCTGGTCCA GTTTGGCACC
    ATGGTCATCG ACCGTGCCCT CTACCTGCGC AAGACTGTCC TGGGAAAGCT GGCCTTTCAG
    GTGGTCCTGG TGGTGGCGAT TCACATCTGG ATGTTCTTTA TCTTACCGGC TGTCACTGAG
    AGGATGTTCA GCCAGAATGC GGTGGCACAG CTGTGGTACT TCGTCAAGTG CATTTACTTT
    GCCCTGTCCG CCTACCAGAT CCGCTGTGGC TACCCCACCC GTATCTTGGG CAACTTCCTC
    ACCAAGAAAT ACAACCATCT AAACCTCTTC CTTTTCCAGG GGTTCCGTCT AGTGCCGTTC
    CTGGTGGAGC TGCGGGCCGT CATGGACTGG GTGTGGACCG ACACCACGCT GTCCCTGTCC
    AACTGGATGT GTGTGGAAGA CATCTATGCC AACATCTTTA TCATCAAGTG CAGCCGAGAG
    ACAGAGAAGA AATACCCCCA GCCCAAGGGG CAGAAGAAGA AGAAAATTGT CAAGTATGGT
    ATGGGAGGCC TCATTATCCT CTTCCTCATC GCCATCATCT GGTTCCCTCT GCTCTTCATG
    TCACTGATCC GCTCTGTGGT CGGGGTCGTC AACCAGCCCA TTGATGTCAC CGTCACCCTC
    AAGCTAGGCG GCTATGAGCC ACTGTTCACC ATGAGCGCCC AGCAGCCATC CATTGTGCCA
    TTCACACCCC AGGCCTACGA GGAGCTGTCC CAGCAGTTTG ACCCCTATCC ACTAGCCATG
    CAGTTCATTA GCCAGTACAG TCCTGAGGAC ATCGTCACTG CACAGATCGA GGGCAGCTCG
    GGGGCGCTGT GGCGCATCAG CCCACCCAGC CGAGCCCAGA TGAAGCAGGA GCTGTACAAC
    GGCACAGCCG ACATTACACT GCGCTTTACC TGGAATTTCC AAAGGGACCT GGCCAAGGGT
    GGCACTGTGG AGTATACTAA TGAGAAGCAC ACCTTGGAGC TGGCCCCCAA CAGTACGGCA
    CGAAGGCAGC TGGCCCAACT GCTCGAGGGC AGACCTGACC AGTCAGTGGT CATTCCCCAT
    CTCTTCCCCA AGTACATTCG TGCTCCCAAT GGGCCTGAAG CCAACCCTGT GAAGCAGCTG
    CAGCCAGATG AGGAAGAGGA CTACCTTGGT GTGCGCATCC AGCTGCGGAG GGAGCAAGTG
    GGCACAGGGG CCTCTGGGGA GCAAGCGGGC ACCAAGGCCT CCGACTTCCT CGAGTGGTGG
    GTCATCGAGC TGCAGGACTG CAAGGCTGAC TGCAACCTGC TGCCCATGGT CATCTTCAGT
    GACAAGGTCA GCCCACCTAG CCTGGGCTTC CTGGCCGGCT ACGGGATTGT GGGGCTGTAC
    GTCTCCATCG TGCTGGTGGT TGGCAAGTTT GTGCGGGGCT TCTTCAGCGA GATCTCTCAC
    TCCATCATGT TCGAGGAACT GCCGTGTGTG GACCGCATCC TCAAGCTGTG CCAGGACATC
    TTCTTGGTGC GCGAGACCCG GGAGCTGGAG CTGGAGGAGG AGCTATACGC CAAGCTCATC
    TTCCTGTACC GATCTCCAGA GACCATGATT AAGTGGACAC GTGAGAGGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006531022.1
    CDS612..6065
    Translation

    Target ORF information:

    RefSeq Version XM_006530959.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530959.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGGCTCAC AGGATGCAGT GAGCGAGGCC CCTCTGCTTG AGCATCAGGA GGAAGAGGAA 
    GTCTTCAGGG AAGATGGGCA GAGCATGGAT GGGCCCCACC AGGCCACACA GGTCCCTGAG
    GGTACGGCCA GCAAGTGGGG CCTGGTGGCT GACCGGCTGC TGGACCTAGC GGCCAGCTTC
    TCGGCTGTCC TCACCCGAAT CCAGGTGTTC GTTCGGCGCT TGCTAGAACT TCACGTCTTC
    AAGCTGGTGG CCCTCTACAC TGTCTGGGTG GCCCTGAAAG AAGTGTCTGT GATGAACCTG
    CTGCTGGTGG TGCTATGGGC CTTCGCCTTG CCCTATCCGC GCTTCCGGCC CATGGCTTCC
    TGCCTGTCCA CCGTGTGGAC CTGTATCATC ATTGTATGCA AGATGCTCTA TCAGCTCAAG
    ATTGTCAACC CGCATGAGTA CTCCAGCAAC TGCACTGAGC CCTTCCCCAA CAATACCAAC
    TTGCAGCCTT TGGAGATCAA CCAGTCTTTG CTGTACCGTG GCCCTGTTGA CCCTGCCAAC
    TGGTTTGGGG TGCGGAAGGG TTACCCCAAC TTGGGCTATA TCCAGAACCA CCTGCAGATC
    CTTCTGTTGC TGGTGTTTGA GGCCGTGGTG TACCGGCGCC AAGAGCACTA CCGCCGGCAG
    CACCAGCAGG CCCCTCTGCC CGCCCAGGCT GTGTGCGCAG ATGGCACCCG CCAGAGGTTG
    GACCAGGACC TACTTAGCTG CCTCAAGTAT TTCATCAACT TCTTCTTCTA CAAATTCGGG
    CTGGAGATCT GCTTCTTGAT GGCCGTGAAT GTGATTGGGC AGCGTATGAA CTTCATGGTG
    ATCTTACACG GTTGCTGGTT GGTGGCCATC CTTACACGCC GGCGCCGTGA GGCCATCGCC
    CGCCTCTGGC CTAACTACTG TCTGTTCCTC ACGCTGTTCC TGCTGTACCA GTACCTGCTG
    TGTTTGGGCA TGCCCCCTGC ACTGTGCATT GACTATCCAT GGCGCTGGAG CAAGGCCATC
    CCCATGAATT CCGCCCTCAT CAAGTGGCTG TACCTACCTG ACTTCTTCAG AGCCCCCAAC
    TCCACCAACC TTATCAGTGA CTTCCTCCTG CTGCTTTGCG CCTCCCAGCA GTGGCAGGTC
    TTCTCAGCGG AGCGAACGGA GGAGTGGCAA CGCATGGCGG GCATCAACAC TGACCACCTG
    GAGCCCCTGC GTGGGGAGCC CAACCCTATA CCCAACTTCA TCCACTGCAG GTCCTATCTG
    GATATGCTGA AGGTGGCCGT CTTCCGCTAC CTGTTCTGGC TGGTGCTCGT TGTGGTGTTT
    GTTGCGGGGG CCACCCGCAT AAGCATCTTC GGGCTGGGGT ACCTGCTAGC CTGCTTCTAC
    CTGCTGCTGT TTGGCACTAC CCTGCTGCAG AAGGACACGC GAGCCCAGCT CGTGCTGTGG
    GACTGCCTCA TCCTCTATAA TGTCACTGTC ATCATCTCTA AGAATATGCT GTCGCTCCTG
    TCCTGTGTCT TCGTGGAGCA AATGCAGAGC AACTTCTGCT GGGTCATCCA GCTCTTCAGC
    CTCGTGTGCA CAGTCAAAGG CTACTATGAT CCCAAAGAGA TGATGACCAG GGACCGGGAC
    TGCCTGCTGC CTGTGGAGGA GGCCGGGATC ATCTGGGACA GTATCTGCTT CTTCTTCCTG
    CTCTTGCAAC GGCGCATCTT TCTCAGCCAC TACTTCCTGC ATGTCAGCGC TGACCTGAAA
    GCCACAGCCC TGCAGGCATC CAGGGGCTTT GCCCTCTACA ATGCAGCCAA CCTGAAGAGC
    ATCAACTTCC ATCGCCAGAT TGAGGAGAAG TCCCTGGCCC AGCTGAAAAG ACAGATGAAG
    CGCATCCGTG CCAAACAGGA GAAGTACAGG CAGAGCCAGG CAAGTCGTGG CCAACTCCAG
    TCCAAAGACC CTCAGGATCC CAGCCAGGAG CCAGGGCCTG ACAGCCCAGG GGGCTCCTCC
    CCGCCACGGA GACAGTGGTG GCGCCCCTGG CTGGACCACG CCACAGTCAT CCACTCTGGC
    GACTACTTCC TGTTTGAGTC AGATAGCGAG GAGGAAGAGG AGGCCCTACC TGAGGACCCC
    AGGCCTGCAG CTCAGAGTGC CTTCCAGATG GCATACCAGG CATGGGTAAC CAATGCCCAG
    ACAGTGCTGA GGCAGCGTCG GGAGCGGGCA CGGCAGGAGC GGGCAGAGCA GCTGGCTTCT
    GGAGGTGACT TGAACCCAGA TGTGGAACCA GTAGATGTCC CAGAAGATGA GATGGCAGGC
    CGTAGCCACA TGATGCAGCG TGTGCTAAGC ACCATGCAGT TCCTGTGGGT GCTGGGCCAG
    GCCACGGTAG ACGGGCTGAC GCGCTGGCTG CGTGCATTCA CGAAGCACCA CCGCACCATG
    AGCGATGTGC TGTGCGCAGA GCGCTACCTG CTCACCCAGG AGCTTCTTCG GGTTGGAGAG
    GTACGCCGAG GTGTGCTGGA CCAGCTTTAT GTGGGTGAAG ATGAGGCCAC ATTGTCAGGT
    CCCATGGAGA CCCGGGATGG ACCCAGCACA GCCTCAAGTG GGCTGGGAGC CGAAGAGCCT
    TTGAGTAGCA TGACAGACGA CACCAGCAGC CCCCTGAGCA CAGGCTATAA CACCCGCAGT
    GGCAGTGAGG AGATTGTCAC CGACGCTGGG GACCTCCAGG CTGGGACCTC CCTGCACGGC
    TCCCAAGAGC TTTTAGCCAA TGCTCGTACC CGGATGCGCA CGGCCAGCGA GCTGCTACTG
    GATAGGCGCC TGCATATCCC TGAGCTGGAG GAGGCCGAGC GGTTTGAGGC ACAGCAGGGC
    CGGACTCTGC GGCTGCTCAG GGCTGGGTAC CAGTGCGTGG CGGCACACTC GGAGCTGCTC
    TGTTACTTCA TCATCATCCT TAACCACATG GTGACAGCCT CGGCTGCCTC CCTGGTGCTG
    CCCGTGCTTG TGTTCCTGTG GGCCATGCTG ACCATCCCGA GGCCTAGCAA GCGCTTTTGG
    ATGACAGCTA TCGTCTTCAC TGAGGTCATG GTGGTCACCA AATACCTGTT CCAGTTCGGC
    TTCTTCCCCT GGAACAGCTA CGTTGTGCTG CGGCGCTATG AGAACAAGCC CTACTTCCCT
    CCGCGAATCC TGGGCCTTGA GAAAACGGAC AGCTACATCA AGTATGACCT GGTGCAGCTC
    ATGGCCCTCT TCTTCCACCG CTCGCAGCTA CTGTGTTATG GCCTCTGGGA CCATGAGGAG
    GATCGCTATC CCAAGGACCA TTGCAGGAGT AGTGTGAAGG ACCGGGAGGC CAAGGAAGAG
    CCAGAAGCTA AGCTGGAATC GCAGTCTGAG ACAGGCACTG GGCATCCCAA GGAGCCAGTG
    TTGGCCGGTA CTCCCAGGGA CCACATCCAA GGGAAAGGAA GTATTAGATC CAAGGATGTT
    ATCCAAGATC CCCCAGAGGA CCTTAAGCCC CGGCACACGA GGCACATCAG CATACGCTTC
    AGGAGGCGCA AGGAGACTCC AGGACCCAAA GGAACAGCAG TCATGGAGAC TGAGCACGAG
    GAGGGAGAAG GAAAAGAAAC TACAGAGAGA AAGAGGCCGC GTCACACTCA AGAAAAATCG
    AAGTTTCGGG AGAGAATGAA GGCAGCTGGG CGCCGGCTGC AGAGCTTCTG TGTGTCACTG
    GCCCAGAGCT TCTACCAACC CTTGCAGCGT TTCTTCCATG ACATTCTGCA CACAAAGTAC
    CGGGCGGCCA CCGACGTCTA CGCCCTCATG TTCCTGGCCG ATATTGTCGA CATCATCATC
    ATCATCTTTG GTTTTTGGGC TTTTGGGAAG CACTCTGCAG CCACAGACAT TGCATCCTCG
    CTGTCAGATG ACCAGGTGCC ACAGGCCTTC CTGTTCATGC TGCTGGTCCA GTTTGGCACC
    ATGGTCATCG ACCGTGCCCT CTACCTGCGC AAGACTGTCC TGGGAAAGCT GGCCTTTCAG
    GTGGTCCTGG TGGTGGCGAT TCACATCTGG ATGTTCTTTA TCTTACCGGC TGTCACTGAG
    AGGATGTTCA GCCAGAATGC GGTGGCACAG CTGTGGTACT TCGTCAAGTG CATTTACTTT
    GCCCTGTCCG CCTACCAGAT CCGCTGTGGC TACCCCACCC GTATCTTGGG CAACTTCCTC
    ACCAAGAAAT ACAACCATCT AAACCTCTTC CTTTTCCAGG GGTTCCGTCT AGTGCCGTTC
    CTGGTGGAGC TGCGGGCCGT CATGGACTGG GTGTGGACCG ACACCACGCT GTCCCTGTCC
    AACTGGATGT GTGTGGAAGA CATCTATGCC AACATCTTTA TCATCAAGTG CAGCCGAGAG
    ACAGAGAAGA AATACCCCCA GCCCAAGGGG CAGAAGAAGA AGAAAATTGT CAAGTATGGT
    ATGGGAGGCC TCATTATCCT CTTCCTCATC GCCATCATCT GGTTCCCTCT GCTCTTCATG
    TCACTGATCC GCTCTGTGGT CGGGGTCGTC AACCAGCCCA TTGATGTCAC CGTCACCCTC
    AAGCTAGGCG GCTATGAGCC ACTGTTCACC ATGAGCGCCC AGCAGCCATC CATTGTGCCA
    TTCACACCCC AGGCCTACGA GGAGCTGTCC CAGCAGTTTG ACCCCTATCC ACTAGCCATG
    CAGTTCATTA GCCAGTACAG TCCTGAGGAC ATCGTCACTG CACAGATCGA GGGCAGCTCG
    GGGGCGCTGT GGCGCATCAG CCCACCCAGC CGAGCCCAGA TGAAGCAGGA GCTGTACAAC
    GGCACAGCCG ACATTACACT GCGCTTTACC TGGAATTTCC AAAGGGACCT GGCCAAGGGT
    GGCACTGTGG AGTATACTAA TGAGAAGCAC ACCTTGGAGC TGGCCCCCAA CAGTACGGCA
    CGAAGGCAGC TGGCCCAACT GCTCGAGGGC AGACCTGACC AGTCAGTGGT CATTCCCCAT
    CTCTTCCCCA AGTACATTCG TGCTCCCAAT GGGCCTGAAG CCAACCCTGT GAAGCAGCTG
    CAGCCAGATG AGGAAGAGGA CTACCTTGGT GTGCGCATCC AGCTGCGGAG GGAGCAAGTG
    GGCACAGGGG CCTCTGGGGA GCAAGCGGGC ACCAAGGCCT CCGACTTCCT CGAGTGGTGG
    GTCATCGAGC TGCAGGACTG CAAGGCTGAC TGCAACCTGC TGCCCATGGT CATCTTCAGT
    GACAAGGTCA GCCCACCTAG CCTGGGCTTC CTGGCCGGCT ACGGGATTGT GGGGCTGTAC
    GTCTCCATCG TGCTGGTGGT TGGCAAGTTT GTGCGGGGCT TCTTCAGCGA GATCTCTCAC
    TCCATCATGT TCGAGGAACT GCCGTGTGTG GACCGCATCC TCAAGCTGTG CCAGGACATC
    TTCTTGGTGC GCGAGACCCG GGAGCTGGAG CTGGAGGAGG AGCTATACGC CAAGCTCATC
    TTCCTGTACC GATCTCCAGA GACCATGATT AAGTGGACAC GTGAGAGGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu73794
    Clone ID Related Accession (Same CDS sequence) XM_011248372.1
    Accession Version XM_011248372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGAAGG TGGCCGTCTT CCGCTACCTG TTCTGGCTGG TGCTCGTTGT GGTGTTTGTT 
    GCGGGGGCCA CCCGCATAAG CATCTTCGGG CTGGGGTACC TGCTAGCCTG CTTCTACCTG
    CTGCTGTTTG GCACTACCCT GCTGCAGAAG GACACGCGAG CCCAGCTCGT GCTGTGGGAC
    TGCCTCATCC TCTATAATGT CACTGTCATC ATCTCTAAGA ATATGCTGTC GCTCCTGTCC
    TGTGTCTTCG TGGAGCAAAT GCAGAGCAAC TTCTGCTGGG TCATCCAGCT CTTCAGCCTC
    GTGTGCACAG TCAAAGGCTA CTATGATCCC AAAGAGATGA TGACCAGGGA CCGGGACTGC
    CTGCTGCCTG TGGAGGAGGC CGGGATCATC TGGGACAGTA TCTGCTTCTT CTTCCTGCTC
    TTGCAACGGC GCATCTTTCT CAGCCACTAC TTCCTGCATG TCAGCGCTGA CCTGAAAGCC
    ACAGCCCTGC AGGCATCCAG GGGCTTTGCC CTCTACAATG CAGCCAACCT GAAGAGCATC
    AACTTCCATC GCCAGATTGA GGAGAAGTCC CTGGCCCAGC TGAAAAGACA GATGAAGCGC
    ATCCGTGCCA AACAGGAGAA GTACAGGCAG AGCCAGGCAA GTCGTGGCCA ACTCCAGTCC
    AAAGACCCTC AGGATCCCAG CCAGGAGCCA GGGCCTGACA GCCCAGGGGG CTCCTCCCCG
    CCACGGAGAC AGTGGTGGCG CCCCTGGCTG GACCACGCCA CAGTCATCCA CTCTGGCGAC
    TACTTCCTGT TTGAGTCAGA TAGCGAGGAG GAAGAGGAGG CCCTACCTGA GGACCCCAGG
    CCTGCAGCTC AGAGTGCCTT CCAGATGGCA TACCAGGCAT GGGTAACCAA TGCCCAGACA
    GTGCTGAGGC AGCGTCGGGA GCGGGCACGG CAGGAGCGGG CAGAGCAGCT GGCTTCTGGA
    GGTGACTTGA ACCCAGATGT GGAACCAGTA GATGTCCCAG AAGATGAGAT GGCAGGCCGT
    AGCCACATGA TGCAGCGTGT GCTAAGCACC ATGCAGTTCC TGTGGGTGCT GGGCCAGGCC
    ACGGTAGACG GGCTGACGCG CTGGCTGCGT GCATTCACGA AGCACCACCG CACCATGAGC
    GATGTGCTGT GCGCAGAGCG CTACCTGCTC ACCCAGGAGC TTCTTCGGGT TGGAGAGGTA
    CGCCGAGGTG TGCTGGACCA GCTTTATGTG GGTGAAGATG AGGCCACATT GTCAGGTCCC
    ATGGAGACCC GGGATGGACC CAGCACAGCC TCAAGTGGGC TGGGAGCCGA AGAGCCTTTG
    AGTAGCATGA CAGACGACAC CAGCAGCCCC CTGAGCACAG GCTATAACAC CCGCAGTGGC
    AGTGAGGAGA TTGTCACCGA CGCTGGGGAC CTCCAGGCTG GGACCTCCCT GCACGGCTCC
    CAAGAGCTTT TAGCCAATGC TCGTACCCGG ATGCGCACGG CCAGCGAGCT GCTACTGGAT
    AGGCGCCTGC ATATCCCTGA GCTGGAGGAG GCCGAGCGGT TTGAGGCACA GCAGGGCCGG
    ACTCTGCGGC TGCTCAGGGC TGGGTACCAG TGCGTGGCGG CACACTCGGA GCTGCTCTGT
    TACTTCATCA TCATCCTTAA CCACATGGTG ACAGCCTCGG CTGCCTCCCT GGTGCTGCCC
    GTGCTTGTGT TCCTGTGGGC CATGCTGACC ATCCCGAGGC CTAGCAAGCG CTTTTGGATG
    ACAGCTATCG TCTTCACTGA GGTCATGGTG GTCACCAAAT ACCTGTTCCA GTTCGGCTTC
    TTCCCCTGGA ACAGCTACGT TGTGCTGCGG CGCTATGAGA ACAAGCCCTA CTTCCCTCCG
    CGAATCCTGG GCCTTGAGAA AACGGACAGC TACATCAAGT ATGACCTGGT GCAGCTCATG
    GCCCTCTTCT TCCACCGCTC GCAGCTACTG TGTTATGGCC TCTGGGACCA TGAGGAGGAT
    CGCTATCCCA AGGACCATTG CAGGAGTAGT GTGAAGGACC GGGAGGCCAA GGAAGAGCCA
    GAAGCTAAGC TGGAATCGCA GTCTGAGACA GGCACTGGGC ATCCCAAGGA GCCAGTGTTG
    GCCGGTACTC CCAGGGACCA CATCCAAGGG AAAGGAAGTA TTAGATCCAA GGATGTTATC
    CAAGATCCCC CAGAGGACCT TAAGCCCCGG CACACGAGGC ACATCAGCAT ACGCTTCAGG
    AGGCGCAAGG AGACTCCAGG ACCCAAAGGA ACAGCAGTCA TGGAGACTGA GCACGAGGAG
    GGAGAAGGAA AAGAAACTAC AGAGAGAAAG AGGCCGCGTC ACACTCAAGA AAAATCGAAG
    TTTCGGGAGA GAATGAAGGC AGCTGGGCGC CGGCTGCAGA GCTTCTGTGT GTCACTGGCC
    CAGAGCTTCT ACCAACCCTT GCAGCGTTTC TTCCATGACA TTCTGCACAC AAAGTACCGG
    GCGGCCACCG ACGTCTACGC CCTCATGTTC CTGGCCGATA TTGTCGACAT CATCATCATC
    ATCTTTGGTT TTTGGGCTTT TGGGAAGCAC TCTGCAGCCA CAGACATTGC ATCCTCGCTG
    TCAGATGACC AGGTGCCACA GGCCTTCCTG TTCATGCTGC TGGTCCAGTT TGGCACCATG
    GTCATCGACC GTGCCCTCTA CCTGCGCAAG ACTGTCCTGG GAAAGCTGGC CTTTCAGGTG
    GTCCTGGTGG TGGCGATTCA CATCTGGATG TTCTTTATCT TACCGGCTGT CACTGAGAGG
    ATGTTCAGCC AGAATGCGGT GGCACAGCTG TGGTACTTCG TCAAGTGCAT TTACTTTGCC
    CTGTCCGCCT ACCAGATCCG CTGTGGCTAC CCCACCCGTA TCTTGGGCAA CTTCCTCACC
    AAGAAATACA ACCATCTAAA CCTCTTCCTT TTCCAGGGGT TCCGTCTAGT GCCGTTCCTG
    GTGGAGCTGC GGGCCGTCAT GGACTGGGTG TGGACCGACA CCACGCTGTC CCTGTCCAAC
    TGGATGTGTG TGGAAGACAT CTATGCCAAC ATCTTTATCA TCAAGTGCAG CCGAGAGACA
    GAGAAGAAAT ACCCCCAGCC CAAGGGGCAG AAGAAGAAGA AAATTGTCAA GTATGGTATG
    GGAGGCCTCA TTATCCTCTT CCTCATCGCC ATCATCTGGT TCCCTCTGCT CTTCATGTCA
    CTGATCCGCT CTGTGGTCGG GGTCGTCAAC CAGCCCATTG ATGTCACCGT CACCCTCAAG
    CTAGGCGGCT ATGAGCCACT GTTCACCATG AGCGCCCAGC AGCCATCCAT TGTGCCATTC
    ACACCCCAGG CCTACGAGGA GCTGTCCCAG CAGTTTGACC CCTATCCACT AGCCATGCAG
    TTCATTAGCC AGTACAGTCC TGAGGACATC GTCACTGCAC AGATCGAGGG CAGCTCGGGG
    GCGCTGTGGC GCATCAGCCC ACCCAGCCGA GCCCAGATGA AGCAGGAGCT GTACAACGGC
    ACAGCCGACA TTACACTGCG CTTTACCTGG AATTTCCAAA GGGACCTGGC CAAGGGTGGC
    ACTGTGGAGT ATACTAATGA GAAGCACACC TTGGAGCTGG CCCCCAACAG TACGGCACGA
    AGGCAGCTGG CCCAACTGCT CGAGGGCAGA CCTGACCAGT CAGTGGTCAT TCCCCATCTC
    TTCCCCAAGT ACATTCGTGC TCCCAATGGG CCTGAAGCCA ACCCTGTGAA GCAGCTGCAG
    CCAGATGAGG AAGAGGACTA CCTTGGTGTG CGCATCCAGC TGCGGAGGGA GCAAGTGGGC
    ACAGGGGCCT CTGGGGAGCA AGCGGGCACC AAGGCCTCCG ACTTCCTCGA GTGGTGGGTC
    ATCGAGCTGC AGGACTGCAA GGCTGACTGC AACCTGCTGC CCATGGTCAT CTTCAGTGAC
    AAGGTCAGCC CACCTAGCCT GGGCTTCCTG GCCGGCTACG GGATTGTGGG GCTGTACGTC
    TCCATCGTGC TGGTGGTTGG CAAGTTTGTG CGGGGCTTCT TCAGCGAGAT CTCTCACTCC
    ATCATGTTCG AGGAACTGCC GTGTGTGGAC CGCATCCTCA AGCTGTGCCA GGACATCTTC
    TTGGTGCGCG AGACCCGGGA GCTGGAGCTG GAGGAGGAGC TATACGCCAA GCTCATCTTC
    CTGTACCGAT CTCCAGAGAC CATGATTAAG TGGACACGTG AGAGGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011246674.1
    CDS130..4320
    Translation

    Target ORF information:

    RefSeq Version XM_011248372.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011248372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGAAGG TGGCCGTCTT CCGCTACCTG TTCTGGCTGG TGCTCGTTGT GGTGTTTGTT 
    GCGGGGGCCA CCCGCATAAG CATCTTCGGG CTGGGGTACC TGCTAGCCTG CTTCTACCTG
    CTGCTGTTTG GCACTACCCT GCTGCAGAAG GACACGCGAG CCCAGCTCGT GCTGTGGGAC
    TGCCTCATCC TCTATAATGT CACTGTCATC ATCTCTAAGA ATATGCTGTC GCTCCTGTCC
    TGTGTCTTCG TGGAGCAAAT GCAGAGCAAC TTCTGCTGGG TCATCCAGCT CTTCAGCCTC
    GTGTGCACAG TCAAAGGCTA CTATGATCCC AAAGAGATGA TGACCAGGGA CCGGGACTGC
    CTGCTGCCTG TGGAGGAGGC CGGGATCATC TGGGACAGTA TCTGCTTCTT CTTCCTGCTC
    TTGCAACGGC GCATCTTTCT CAGCCACTAC TTCCTGCATG TCAGCGCTGA CCTGAAAGCC
    ACAGCCCTGC AGGCATCCAG GGGCTTTGCC CTCTACAATG CAGCCAACCT GAAGAGCATC
    AACTTCCATC GCCAGATTGA GGAGAAGTCC CTGGCCCAGC TGAAAAGACA GATGAAGCGC
    ATCCGTGCCA AACAGGAGAA GTACAGGCAG AGCCAGGCAA GTCGTGGCCA ACTCCAGTCC
    AAAGACCCTC AGGATCCCAG CCAGGAGCCA GGGCCTGACA GCCCAGGGGG CTCCTCCCCG
    CCACGGAGAC AGTGGTGGCG CCCCTGGCTG GACCACGCCA CAGTCATCCA CTCTGGCGAC
    TACTTCCTGT TTGAGTCAGA TAGCGAGGAG GAAGAGGAGG CCCTACCTGA GGACCCCAGG
    CCTGCAGCTC AGAGTGCCTT CCAGATGGCA TACCAGGCAT GGGTAACCAA TGCCCAGACA
    GTGCTGAGGC AGCGTCGGGA GCGGGCACGG CAGGAGCGGG CAGAGCAGCT GGCTTCTGGA
    GGTGACTTGA ACCCAGATGT GGAACCAGTA GATGTCCCAG AAGATGAGAT GGCAGGCCGT
    AGCCACATGA TGCAGCGTGT GCTAAGCACC ATGCAGTTCC TGTGGGTGCT GGGCCAGGCC
    ACGGTAGACG GGCTGACGCG CTGGCTGCGT GCATTCACGA AGCACCACCG CACCATGAGC
    GATGTGCTGT GCGCAGAGCG CTACCTGCTC ACCCAGGAGC TTCTTCGGGT TGGAGAGGTA
    CGCCGAGGTG TGCTGGACCA GCTTTATGTG GGTGAAGATG AGGCCACATT GTCAGGTCCC
    ATGGAGACCC GGGATGGACC CAGCACAGCC TCAAGTGGGC TGGGAGCCGA AGAGCCTTTG
    AGTAGCATGA CAGACGACAC CAGCAGCCCC CTGAGCACAG GCTATAACAC CCGCAGTGGC
    AGTGAGGAGA TTGTCACCGA CGCTGGGGAC CTCCAGGCTG GGACCTCCCT GCACGGCTCC
    CAAGAGCTTT TAGCCAATGC TCGTACCCGG ATGCGCACGG CCAGCGAGCT GCTACTGGAT
    AGGCGCCTGC ATATCCCTGA GCTGGAGGAG GCCGAGCGGT TTGAGGCACA GCAGGGCCGG
    ACTCTGCGGC TGCTCAGGGC TGGGTACCAG TGCGTGGCGG CACACTCGGA GCTGCTCTGT
    TACTTCATCA TCATCCTTAA CCACATGGTG ACAGCCTCGG CTGCCTCCCT GGTGCTGCCC
    GTGCTTGTGT TCCTGTGGGC CATGCTGACC ATCCCGAGGC CTAGCAAGCG CTTTTGGATG
    ACAGCTATCG TCTTCACTGA GGTCATGGTG GTCACCAAAT ACCTGTTCCA GTTCGGCTTC
    TTCCCCTGGA ACAGCTACGT TGTGCTGCGG CGCTATGAGA ACAAGCCCTA CTTCCCTCCG
    CGAATCCTGG GCCTTGAGAA AACGGACAGC TACATCAAGT ATGACCTGGT GCAGCTCATG
    GCCCTCTTCT TCCACCGCTC GCAGCTACTG TGTTATGGCC TCTGGGACCA TGAGGAGGAT
    CGCTATCCCA AGGACCATTG CAGGAGTAGT GTGAAGGACC GGGAGGCCAA GGAAGAGCCA
    GAAGCTAAGC TGGAATCGCA GTCTGAGACA GGCACTGGGC ATCCCAAGGA GCCAGTGTTG
    GCCGGTACTC CCAGGGACCA CATCCAAGGG AAAGGAAGTA TTAGATCCAA GGATGTTATC
    CAAGATCCCC CAGAGGACCT TAAGCCCCGG CACACGAGGC ACATCAGCAT ACGCTTCAGG
    AGGCGCAAGG AGACTCCAGG ACCCAAAGGA ACAGCAGTCA TGGAGACTGA GCACGAGGAG
    GGAGAAGGAA AAGAAACTAC AGAGAGAAAG AGGCCGCGTC ACACTCAAGA AAAATCGAAG
    TTTCGGGAGA GAATGAAGGC AGCTGGGCGC CGGCTGCAGA GCTTCTGTGT GTCACTGGCC
    CAGAGCTTCT ACCAACCCTT GCAGCGTTTC TTCCATGACA TTCTGCACAC AAAGTACCGG
    GCGGCCACCG ACGTCTACGC CCTCATGTTC CTGGCCGATA TTGTCGACAT CATCATCATC
    ATCTTTGGTT TTTGGGCTTT TGGGAAGCAC TCTGCAGCCA CAGACATTGC ATCCTCGCTG
    TCAGATGACC AGGTGCCACA GGCCTTCCTG TTCATGCTGC TGGTCCAGTT TGGCACCATG
    GTCATCGACC GTGCCCTCTA CCTGCGCAAG ACTGTCCTGG GAAAGCTGGC CTTTCAGGTG
    GTCCTGGTGG TGGCGATTCA CATCTGGATG TTCTTTATCT TACCGGCTGT CACTGAGAGG
    ATGTTCAGCC AGAATGCGGT GGCACAGCTG TGGTACTTCG TCAAGTGCAT TTACTTTGCC
    CTGTCCGCCT ACCAGATCCG CTGTGGCTAC CCCACCCGTA TCTTGGGCAA CTTCCTCACC
    AAGAAATACA ACCATCTAAA CCTCTTCCTT TTCCAGGGGT TCCGTCTAGT GCCGTTCCTG
    GTGGAGCTGC GGGCCGTCAT GGACTGGGTG TGGACCGACA CCACGCTGTC CCTGTCCAAC
    TGGATGTGTG TGGAAGACAT CTATGCCAAC ATCTTTATCA TCAAGTGCAG CCGAGAGACA
    GAGAAGAAAT ACCCCCAGCC CAAGGGGCAG AAGAAGAAGA AAATTGTCAA GTATGGTATG
    GGAGGCCTCA TTATCCTCTT CCTCATCGCC ATCATCTGGT TCCCTCTGCT CTTCATGTCA
    CTGATCCGCT CTGTGGTCGG GGTCGTCAAC CAGCCCATTG ATGTCACCGT CACCCTCAAG
    CTAGGCGGCT ATGAGCCACT GTTCACCATG AGCGCCCAGC AGCCATCCAT TGTGCCATTC
    ACACCCCAGG CCTACGAGGA GCTGTCCCAG CAGTTTGACC CCTATCCACT AGCCATGCAG
    TTCATTAGCC AGTACAGTCC TGAGGACATC GTCACTGCAC AGATCGAGGG CAGCTCGGGG
    GCGCTGTGGC GCATCAGCCC ACCCAGCCGA GCCCAGATGA AGCAGGAGCT GTACAACGGC
    ACAGCCGACA TTACACTGCG CTTTACCTGG AATTTCCAAA GGGACCTGGC CAAGGGTGGC
    ACTGTGGAGT ATACTAATGA GAAGCACACC TTGGAGCTGG CCCCCAACAG TACGGCACGA
    AGGCAGCTGG CCCAACTGCT CGAGGGCAGA CCTGACCAGT CAGTGGTCAT TCCCCATCTC
    TTCCCCAAGT ACATTCGTGC TCCCAATGGG CCTGAAGCCA ACCCTGTGAA GCAGCTGCAG
    CCAGATGAGG AAGAGGACTA CCTTGGTGTG CGCATCCAGC TGCGGAGGGA GCAAGTGGGC
    ACAGGGGCCT CTGGGGAGCA AGCGGGCACC AAGGCCTCCG ACTTCCTCGA GTGGTGGGTC
    ATCGAGCTGC AGGACTGCAA GGCTGACTGC AACCTGCTGC CCATGGTCAT CTTCAGTGAC
    AAGGTCAGCC CACCTAGCCT GGGCTTCCTG GCCGGCTACG GGATTGTGGG GCTGTACGTC
    TCCATCGTGC TGGTGGTTGG CAAGTTTGTG CGGGGCTTCT TCAGCGAGAT CTCTCACTCC
    ATCATGTTCG AGGAACTGCC GTGTGTGGAC CGCATCCTCA AGCTGTGCCA GGACATCTTC
    TTGGTGCGCG AGACCCGGGA GCTGGAGCTG GAGGAGGAGC TATACGCCAA GCTCATCTTC
    CTGTACCGAT CTCCAGAGAC CATGATTAAG TGGACACGTG AGAGGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu73789
    Clone ID Related Accession (Same CDS sequence) XM_006530953.2
    Accession Version XM_006530953.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGGTATTC TGTCTGTGGT GCACAAGCCT TATGTATGCA TGCTTACAGC CTCCCTGTTA 
    CGCTTCAATG CTCTCTCGCT GGTCTATTTG CTGTTTCTAC TGCTGCTGCC CTGGCTTCCA
    GGCCCCTCAA GACACAGCAT ACCAGGTCAC ACAGGTCGCC TGCTCCGTGC ACTACTCTGC
    CTCAGCCTCC TCTTCCTGGT GGCCCACCTT GCCTTTCAGA TATGCCTACA CACCGTGCCT
    CACCTGGACC AGTTTCTGGG ACAAAACGGT AGCCTTTGGG TGAAGGTGTC TCAACACATA
    GGGGTTACGA GGCTGGACCT GAAGGACATC TTTAACACCA CCAGGCTGGT AGCACCTGAC
    CTGGGAGTGC TGCTGGCGTC CTCCCTTTGC CTTGGCCTCT GTGGACGCCT CACGAGGAAA
    GCCCGGCAGA GTCGGCGCAC CCAGGAGCTG CAGGATGATG ACGATGACGA TGATGACGAC
    GATGAAGACA TAGATGCTGC CCCAGCCGTG GGGCTGAAGG GAGCCCCTGC CCTGGCAACC
    AAACGCAGGC TGTGGCTGGC CTCCCGCTTC CGGGTCACGG CCCACTGGCT GCTGATGACC
    TCTGGACGGA CGCTGGTCAT TGTGTTGCTG GCCCTGGCAG GCATAGCCCA CCCTTCGGCC
    TTCTCCAGCG TCTACCTGGT GGTGTTCCTG GCCATCTGCA CCTGGTGGTC CTGTCACTTT
    CCTCTCAGCC CCCTGGGCTT TAACACCCTC TGTGTCATGG TGAGCTGTTT TGGTGCCGGC
    CATCTCATTT GCCTATACTG CTATCAGACA CCATTTATCC AGGACATGTT ACCCCCTGGG
    AACATCTGGG CCAGGCTATT TGGTCTCAAG AACTTCGTAG ACCTCCCTAA CTACTCCAGC
    CCCAACGCCC TGGTACTCAA CACTAAGCAC GCCTGGCCCA TCTATGTGAG TCCCGGAATC
    CTGCTGCTGC TATATTACAC AGCCACCTCT CTCCTGAAGC TCCACAAGAG CTGTCCCTCA
    GAGCTGAGGA AGGAGACACC CAGGGAGGAT GAGGAGCATG AGCTGGAACT GGACCACCTG
    GAGCCAGAGC CCCAGGCTAG GGACGCCACC CAGGGTGAGA TGCCCATGAC CACGGAACCT
    GACCTTGACA ACTGCACCGT GCATGTACTA ACCAGCCAGA GCCCTGTCCG CCAGCGTCCA
    GTTCGCCCCA GGCTGGCTGA GCTGAAAGAG ATGTCACCGC TACATGGCCT GGGCCACCTC
    ATCATGGACC AGAGCTATGT GTGTGCCCTG ATTGCCATGA TGGTGTGGAG CATCATGTAC
    CACAGCTGGC TGACCTTCGT CCTGCTGCTC TGGGCCTGCC TCATCTGGAC GGTGCGTAGC
    CGCCACCAGC TGGCTATGCT CTGCTCGCCC TGCATCCTGC TGTATGGGCT GACGCTCTGC
    TGCCTGAGGT ACGTGTGGGC CATGGAACTT CCTGAGCTGC CCACCACCCT GGGCCCTGTC
    AGCCTGCACC AGTTGGGACT GGAACACACA CGCTACCCTT GCCTGGACCT CGGTGCCATG
    CTCCTCTATC TGCTCACATT CTGGCTCCTT CTGCGTCAGT TCGTGAAGGA GAAGCTGCTG
    AAGAAGCAAA AGGTGCCTGC GGCGCTTTTG GAGGTCACGG TGGCCGACAC TGAGCCCACA
    CAGACCCAGA CGCTGCTGCG GAGCCTGGGG GAGCTGGTCA CCGGCATCTA CGTCAAATAC
    TGGATCTATG TGTGCGCCGG CATGTTCATT GTGGTCAGCT TCGCCGGCCG CCTGGTGGTC
    TACAAAATCG TCTACATGTT CCTCTTCCTG CTGTGCCTCA CCTTGTTCCA GGTCTACTAC
    ACCCTGTGGA GGAAGCTGCT GCGTGTCTTC TGGTGGCTCG TGGTGGCCTA TACAATGCTC
    GTGCTCATCG CTGTGTACAC CTTCCAGTTC CAGGACTTCC CCACCTATTG GCGCAACCTC
    ACGGGCTTCA CGGACGAGCA GTTGGGCGAC CTGGGCCTGG AGCAGTTCAG TGTGTCGGAG
    CTCTTTTCCA GTATCCTCAT CCCTGGCTTC TTCCTGCTGG CCTGCATCCT GCAGCTGCAC
    TACTTCCACA GACCGTTCAT GCAGCTCACT GACCTGGAGC ACGTGCCGCC ACCAGGCACC
    CGCCACCCTC GATGGGCTCA CAGGCAGGAT GCAGTGAGCG AGGCCCCTCT GCTTGAGCAT
    CAGGAGGAAG AGGAAGTCTT CAGGGAAGAT GGGCAGAGCA TGGATGGGCC CCACCAGGCC
    ACACAGGTCC CTGAGGGTAC GGCCAGCAAG TGGGGCCTGG TGGCTGACCG GCTGCTGGAC
    CTAGCGGCCA GCTTCTCGGC TGTCCTCACC CGAATCCAGG TGTTCGTTCG GCGCTTGCTA
    GAACTTCACG TCTTCAAGCT GGTGGCCCTC TACACTGTCT GGGTGGCCCT GAAAGAAGTG
    TCTGTGATGA ACCTGCTGCT GGTGGTGCTA TGGGCCTTCG CCTTGCCCTA TCCGCGCTTC
    CGGCCCATGG CTTCCTGCCT GTCCACCGTG TGGACCTGTA TCATCATTGT ATGCAAGATG
    CTCTATCAGC TCAAGATTGT CAACCCGCAT GAGTACTCCA GCAACTGCAC TGAGCCCTTC
    CCCAACAATA CCAACTTGCA GCCTTTGGAG ATCAACCAGT CTTTGCTGTA CCGTGGCCCT
    GTTGACCCTG CCAACTGGTT TGGGGTGCGG AAGGGTTACC CCAACTTGGG CTATATCCAG
    AACCACCTGC AGATCCTTCT GTTGCTGGTG TTTGAGGCCG TGGTGTACCG GCGCCAAGAG
    CACTACCGCC GGCAGCACCA GCAGGCCCCT CTGCCCGCCC AGGCTGTGTG CGCAGATGGC
    ACCCGCCAGA GGTTGGACCA GGACCTACTT AGCTGCCTCA AGTATTTCAT CAACTTCTTC
    TTCTACAAAT TCGGGCTGGA GATCTGCTTC TTGATGGCCG TGAATGTGAT TGGGCAGCGT
    ATGAACTTCA TGGTGATCTT ACACGGTTGC TGGTTGGTGG CCATCCTTAC ACGCCGGCGC
    CGTGAGGCCA TCGCCCGCCT CTGGCCTAAC TACTGTCTGT TCCTCACGCT GTTCCTGCTG
    TACCAGTACC TGCTGTGTTT GGGCATGCCC CCTGCACTGT GCATTGACTA TCCATGGCGC
    TGGAGCAAGG CCATCCCCAT GAATTCCGCC CTCATCAAGT GGCTGTACCT ACCTGACTTC
    TTCAGAGCCC CCAACTCCAC CAACCTTATC AGTGACTTCC TCCTGCTGCT TTGCGCCTCC
    CAGCAGTGGC AGGTCTTCTC AGCGGAGCGA ACGGAGGAGT GGCAACGCAT GGCGGGCATC
    AACACTGACC ACCTGGAGCC CCTGCGTGGG GAGCCCAACC CTATACCCAA CTTCATCCAC
    TGCAGGTCCT ATCTGGATAT GCTGAAGGTG GCCGTCTTCC GCTACCTGTT CTGGCTGGTG
    CTCGTTGTGG TGTTTGTTGC GGGGGCCACC CGCATAAGCA TCTTCGGGCT GGGGTACCTG
    CTAGCCTGCT TCTACCTGCT GCTGTTTGGC ACTACCCTGC TGCAGAAGGA CACGCGAGCC
    CAGCTCGTGC TGTGGGACTG CCTCATCCTC TATAATGTCA CTGTCATCAT CTCTAAGAAT
    ATGCTGTCGC TCCTGTCCTG TGTCTTCGTG GAGCAAATGC AGAGCAACTT CTGCTGGGTC
    ATCCAGCTCT TCAGCCTCGT GTGCACAGTC AAAGGCTACT ATGATCCCAA AGAGATGATG
    ACCAGGGACC GGGACTGCCT GCTGCCTGTG GAGGAGGCCG GGATCATCTG GGACAGTATC
    TGCTTCTTCT TCCTGCTCTT GCAACGGCGC ATCTTTCTCA GCCACTACTT CCTGCATGTC
    AGCGCTGACC TGAAAGCCAC AGCCCTGCAG GCATCCAGGG GCTTTGCCCT CTACAATGCA
    GCCAACCTGA AGAGCATCAA CTTCCATCGC CAGATTGAGG AGAAGTCCCT GGCCCAGCTG
    AAAAGACAGA TGAAGCGCAT CCGTGCCAAA CAGGAGAAGT ACAGGCAGAG CCAGGCAAGT
    CGTGGCCAAC TCCAGTCCAA AGACCCTCAG GATCCCAGCC AGGAGCCAGG GCCTGACAGC
    CCAGGGGGCT CCTCCCCGCC ACGGAGACAG TGGTGGCGCC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGCGACTA CTTCCTGTTT GAGTCAGATA GCGAGGAGGA AGAGGAGGCC
    CTACCTGAGG ACCCCAGGCC TGCAGCTCAG AGTGCCTTCC AGATGGCATA CCAGGCATGG
    GTAACCAATG CCCAGACAGT GCTGAGGCAG CGTCGGGAGC GGGCACGGCA GGAGCGGGCA
    GAGCAGCTGG CTTCTGGAGG TGACTTGAAC CCAGATGTGG AACCAGTAGA TGTCCCAGAA
    GATGAGATGG CAGGCCGTAG CCACATGATG CAGCGTGTGC TAAGCACCAT GCAGTTCCTG
    TGGGTGCTGG GCCAGGCCAC GGTAGACGGG CTGACGCGCT GGCTGCGTGC ATTCACGAAG
    CACCACCGCA CCATGAGCGA TGTGCTGTGC GCAGAGCGCT ACCTGCTCAC CCAGGAGCTT
    CTTCGGGTTG GAGAGGTACG CCGAGGTGTG CTGGACCAGC TTTATGTGGG TGAAGATGAG
    GCCACATTGT CAGGTCCCAT GGAGACCCGG GATGGACCCA GCACAGCCTC AAGTGGGCTG
    GGAGCCGAAG AGCCTTTGAG TAGCATGACA GACGACACCA GCAGCCCCCT GAGCACAGGC
    TATAACACCC GCAGTGGCAG TGAGGAGATT GTCACCGACG CTGGGGACCT CCAGGCTGGG
    ACCTCCCTGC ACGGCTCCCA AGAGCTTTTA GCCAATGCTC GTACCCGGAT GCGCACGGCC
    AGCGAGCTGC TACTGGATAG GCGCCTGCAT ATCCCTGAGC TGGAGGAGGC CGAGCGGTTT
    GAGGCACAGC AGGGCCGGAC TCTGCGGCTG CTCAGGGCTG GGTACCAGTG CGTGGCGGCA
    CACTCGGAGC TGCTCTGTTA CTTCATCATC ATCCTTAACC ACATGGTGAC AGCCTCGGCT
    GCCTCCCTGG TGCTGCCCGT GCTTGTGTTC CTGTGGGCCA TGCTGACCAT CCCGAGGCCT
    AGCAAGCGCT TTTGGATGAC AGCTATCGTC TTCACTGAGG TCATGGTGGT CACCAAATAC
    CTGTTCCAGT TCGGCTTCTT CCCCTGGAAC AGCTACGTTG TGCTGCGGCG CTATGAGAAC
    AAGCCCTACT TCCCTCCGCG AATCCTGGGC CTTGAGAAAA CGGACAGCTA CATCAAGTAT
    GACCTGGTGC AGCTCATGGC CCTCTTCTTC CACCGCTCGC AGCTACTGTG TTATGGCCTC
    TGGGACCATG AGGAGGATCG CTATCCCAAG GACCATTGCA GGAGTAGTGT GAAGGACCGG
    GAGGCCAAGG AAGAGCCAGA AGCTAAGCTG GAATCGCAGT CTGAGACAGG CACTGGGCAT
    CCCAAGGAGC CAGTGTTGGC CGGTACTCCC AGGGACCACA TCCAAGGGAA AGGAAGTATT
    AGATCCAAGG ATGTTATCCA AGATCCCCCA GAGGACCTTA AGCCCCGGCA CACGAGGCAC
    ATCAGCATAC GCTTCAGGAG GCGCAAGGAG ACTCCAGGAC CCAAAGGAAC AGCAGTCATG
    GAGACTGAGC ACGAGGAGGG AGAAGGAAAA GAAACTACAG AGAGAAAGAG GCCGCGTCAC
    ACTCAAGAAA AATCGAAGTT TCGGGAGAGA ATGAAGGCAG CTGGGCGCCG GCTGCAGAGC
    TTCTGTGTGT CACTGGCCCA GAGCTTCTAC CAACCCTTGC AGCGTTTCTT CCATGACATT
    CTGCACACAA AGTACCGGGC GGCCACCGAC GTCTACGCCC TCATGTTCCT GGCCGATATT
    GTCGACATCA TCATCATCAT CTTTGGTTTT TGGGCTTTTG GGAAGCACTC TGCAGCCACA
    GACATTGCAT CCTCGCTGTC AGATGACCAG GTGCCACAGG CCTTCCTGTT CATGCTGCTG
    GTCCAGTTTG GCACCATGGT CATCGACCGT GCCCTCTACC TGCGCAAGAC TGTCCTGGGA
    AAGCTGGCCT TTCAGGTGGT CCTGGTGGTG GCGATTCACA TCTGGATGTT CTTTATCTTA
    CCGGCTGTCA CTGAGAGGAT GTTCAGCCAG AATGCGGTGG CACAGCTGTG GTACTTCGTC
    AAGTGCATTT ACTTTGCCCT GTCCGCCTAC CAGATCCGCT GTGGCTACCC CACCCGTATC
    TTGGGCAACT TCCTCACCAA GAAATACAAC CATCTAAACC TCTTCCTTTT CCAGGGGTTC
    CGTCTAGTGC CGTTCCTGGT GGAGCTGCGG GCCGTCATGG ACTGGGTGTG GACCGACACC
    ACGCTGTCCC TGTCCAACTG GATGTGTGTG GAAGACATCT ATGCCAACAT CTTTATCATC
    AAGTGCAGCC GAGAGACAGA GAAGAAATAC CCCCAGCCCA AGGGGCAGAA GAAGAAGAAA
    ATTGTCAAGT ATGGTATGGG AGGCCTCATT ATCCTCTTCC TCATCGCCAT CATCTGGTTC
    CCTCTGCTCT TCATGTCACT GATCCGCTCT GTGGTCGGGG TCGTCAACCA GCCCATTGAT
    GTCACCGTCA CCCTCAAGCT AGGCGGCTAT GAGCCACTGT TCACCATGAG CGCCCAGCAG
    CCATCCATTG TGCCATTCAC ACCCCAGGCC TACGAGGAGC TGTCCCAGCA GTTTGACCCC
    TATCCACTAG CCATGCAGTT CATTAGCCAG TACAGTCCTG AGGACATCGT CACTGCACAG
    ATCGAGGGCA GCTCGGGGGC GCTGTGGCGC ATCAGCCCAC CCAGCCGAGC CCAGATGAAG
    CAGGAGCTGT ACAACGGCAC AGCCGACATT ACACTGCGCT TTACCTGGAA TTTCCAAAGG
    GACCTGGCCA AGGGTGGCAC TGTGGAGTAT ACTAATGAGA AGCACACCTT GGAGCTGGCC
    CCCAACAGTA CGGCACGAAG GCAGCTGGCC CAACTGCTCG AGGGCAGACC TGACCAGTCA
    GTGGTCATTC CCCATCTCTT CCCCAAGTAC ATTCGTGCTC CCAATGGGCC TGAAGCCAAC
    CCTGTGAAGC AGCTGCAGCC AGATGAGGAA GAGGACTACC TTGGTGTGCG CATCCAGCTG
    CGGAGGGAGC AAGTGGGCAC AGGGGCCTCT GGGGAGCAAG CGGGCACCAA GGCCTCCGAC
    TTCCTCGAGT GGTGGGTCAT CGAGCTGCAG GACTGCAAGG CTGACTGCAA CCTGCTGCCC
    ATGGTCATCT TCAGTGACAA GGTCAGCCCA CCTAGCCTGG GCTTCCTGGC CGGCTACGGG
    ATTGTGGGGC TGTACGTCTC CATCGTGCTG GTGGTTGGCA AGTTTGTGCG GGGCTTCTTC
    AGCGAGATCT CTCACTCCAT CATGTTCGAG GAACTGCCGT GTGTGGACCG CATCCTCAAG
    CTGTGCCAGG ACATCTTCTT GGTGCGCGAG ACCCGGGAGC TGGAGCTGGA GGAGGAGCTA
    TACGCCAAGC TCATCTTCCT GTACCGATCT CCAGAGACCA TGATTAAGTG GACACGTGAG
    AGGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006531016.2
    CDS16..7644
    Translation

    Target ORF information:

    RefSeq Version XM_006530953.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530953.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGGTATTC TGTCTGTGGT GCACAAGCCT TATGTATGCA TGCTTACAGC CTCCCTGTTA 
    CGCTTCAATG CTCTCTCGCT GGTCTATTTG CTGTTTCTAC TGCTGCTGCC CTGGCTTCCA
    GGCCCCTCAA GACACAGCAT ACCAGGTCAC ACAGGTCGCC TGCTCCGTGC ACTACTCTGC
    CTCAGCCTCC TCTTCCTGGT GGCCCACCTT GCCTTTCAGA TATGCCTACA CACCGTGCCT
    CACCTGGACC AGTTTCTGGG ACAAAACGGT AGCCTTTGGG TGAAGGTGTC TCAACACATA
    GGGGTTACGA GGCTGGACCT GAAGGACATC TTTAACACCA CCAGGCTGGT AGCACCTGAC
    CTGGGAGTGC TGCTGGCGTC CTCCCTTTGC CTTGGCCTCT GTGGACGCCT CACGAGGAAA
    GCCCGGCAGA GTCGGCGCAC CCAGGAGCTG CAGGATGATG ACGATGACGA TGATGACGAC
    GATGAAGACA TAGATGCTGC CCCAGCCGTG GGGCTGAAGG GAGCCCCTGC CCTGGCAACC
    AAACGCAGGC TGTGGCTGGC CTCCCGCTTC CGGGTCACGG CCCACTGGCT GCTGATGACC
    TCTGGACGGA CGCTGGTCAT TGTGTTGCTG GCCCTGGCAG GCATAGCCCA CCCTTCGGCC
    TTCTCCAGCG TCTACCTGGT GGTGTTCCTG GCCATCTGCA CCTGGTGGTC CTGTCACTTT
    CCTCTCAGCC CCCTGGGCTT TAACACCCTC TGTGTCATGG TGAGCTGTTT TGGTGCCGGC
    CATCTCATTT GCCTATACTG CTATCAGACA CCATTTATCC AGGACATGTT ACCCCCTGGG
    AACATCTGGG CCAGGCTATT TGGTCTCAAG AACTTCGTAG ACCTCCCTAA CTACTCCAGC
    CCCAACGCCC TGGTACTCAA CACTAAGCAC GCCTGGCCCA TCTATGTGAG TCCCGGAATC
    CTGCTGCTGC TATATTACAC AGCCACCTCT CTCCTGAAGC TCCACAAGAG CTGTCCCTCA
    GAGCTGAGGA AGGAGACACC CAGGGAGGAT GAGGAGCATG AGCTGGAACT GGACCACCTG
    GAGCCAGAGC CCCAGGCTAG GGACGCCACC CAGGGTGAGA TGCCCATGAC CACGGAACCT
    GACCTTGACA ACTGCACCGT GCATGTACTA ACCAGCCAGA GCCCTGTCCG CCAGCGTCCA
    GTTCGCCCCA GGCTGGCTGA GCTGAAAGAG ATGTCACCGC TACATGGCCT GGGCCACCTC
    ATCATGGACC AGAGCTATGT GTGTGCCCTG ATTGCCATGA TGGTGTGGAG CATCATGTAC
    CACAGCTGGC TGACCTTCGT CCTGCTGCTC TGGGCCTGCC TCATCTGGAC GGTGCGTAGC
    CGCCACCAGC TGGCTATGCT CTGCTCGCCC TGCATCCTGC TGTATGGGCT GACGCTCTGC
    TGCCTGAGGT ACGTGTGGGC CATGGAACTT CCTGAGCTGC CCACCACCCT GGGCCCTGTC
    AGCCTGCACC AGTTGGGACT GGAACACACA CGCTACCCTT GCCTGGACCT CGGTGCCATG
    CTCCTCTATC TGCTCACATT CTGGCTCCTT CTGCGTCAGT TCGTGAAGGA GAAGCTGCTG
    AAGAAGCAAA AGGTGCCTGC GGCGCTTTTG GAGGTCACGG TGGCCGACAC TGAGCCCACA
    CAGACCCAGA CGCTGCTGCG GAGCCTGGGG GAGCTGGTCA CCGGCATCTA CGTCAAATAC
    TGGATCTATG TGTGCGCCGG CATGTTCATT GTGGTCAGCT TCGCCGGCCG CCTGGTGGTC
    TACAAAATCG TCTACATGTT CCTCTTCCTG CTGTGCCTCA CCTTGTTCCA GGTCTACTAC
    ACCCTGTGGA GGAAGCTGCT GCGTGTCTTC TGGTGGCTCG TGGTGGCCTA TACAATGCTC
    GTGCTCATCG CTGTGTACAC CTTCCAGTTC CAGGACTTCC CCACCTATTG GCGCAACCTC
    ACGGGCTTCA CGGACGAGCA GTTGGGCGAC CTGGGCCTGG AGCAGTTCAG TGTGTCGGAG
    CTCTTTTCCA GTATCCTCAT CCCTGGCTTC TTCCTGCTGG CCTGCATCCT GCAGCTGCAC
    TACTTCCACA GACCGTTCAT GCAGCTCACT GACCTGGAGC ACGTGCCGCC ACCAGGCACC
    CGCCACCCTC GATGGGCTCA CAGGCAGGAT GCAGTGAGCG AGGCCCCTCT GCTTGAGCAT
    CAGGAGGAAG AGGAAGTCTT CAGGGAAGAT GGGCAGAGCA TGGATGGGCC CCACCAGGCC
    ACACAGGTCC CTGAGGGTAC GGCCAGCAAG TGGGGCCTGG TGGCTGACCG GCTGCTGGAC
    CTAGCGGCCA GCTTCTCGGC TGTCCTCACC CGAATCCAGG TGTTCGTTCG GCGCTTGCTA
    GAACTTCACG TCTTCAAGCT GGTGGCCCTC TACACTGTCT GGGTGGCCCT GAAAGAAGTG
    TCTGTGATGA ACCTGCTGCT GGTGGTGCTA TGGGCCTTCG CCTTGCCCTA TCCGCGCTTC
    CGGCCCATGG CTTCCTGCCT GTCCACCGTG TGGACCTGTA TCATCATTGT ATGCAAGATG
    CTCTATCAGC TCAAGATTGT CAACCCGCAT GAGTACTCCA GCAACTGCAC TGAGCCCTTC
    CCCAACAATA CCAACTTGCA GCCTTTGGAG ATCAACCAGT CTTTGCTGTA CCGTGGCCCT
    GTTGACCCTG CCAACTGGTT TGGGGTGCGG AAGGGTTACC CCAACTTGGG CTATATCCAG
    AACCACCTGC AGATCCTTCT GTTGCTGGTG TTTGAGGCCG TGGTGTACCG GCGCCAAGAG
    CACTACCGCC GGCAGCACCA GCAGGCCCCT CTGCCCGCCC AGGCTGTGTG CGCAGATGGC
    ACCCGCCAGA GGTTGGACCA GGACCTACTT AGCTGCCTCA AGTATTTCAT CAACTTCTTC
    TTCTACAAAT TCGGGCTGGA GATCTGCTTC TTGATGGCCG TGAATGTGAT TGGGCAGCGT
    ATGAACTTCA TGGTGATCTT ACACGGTTGC TGGTTGGTGG CCATCCTTAC ACGCCGGCGC
    CGTGAGGCCA TCGCCCGCCT CTGGCCTAAC TACTGTCTGT TCCTCACGCT GTTCCTGCTG
    TACCAGTACC TGCTGTGTTT GGGCATGCCC CCTGCACTGT GCATTGACTA TCCATGGCGC
    TGGAGCAAGG CCATCCCCAT GAATTCCGCC CTCATCAAGT GGCTGTACCT ACCTGACTTC
    TTCAGAGCCC CCAACTCCAC CAACCTTATC AGTGACTTCC TCCTGCTGCT TTGCGCCTCC
    CAGCAGTGGC AGGTCTTCTC AGCGGAGCGA ACGGAGGAGT GGCAACGCAT GGCGGGCATC
    AACACTGACC ACCTGGAGCC CCTGCGTGGG GAGCCCAACC CTATACCCAA CTTCATCCAC
    TGCAGGTCCT ATCTGGATAT GCTGAAGGTG GCCGTCTTCC GCTACCTGTT CTGGCTGGTG
    CTCGTTGTGG TGTTTGTTGC GGGGGCCACC CGCATAAGCA TCTTCGGGCT GGGGTACCTG
    CTAGCCTGCT TCTACCTGCT GCTGTTTGGC ACTACCCTGC TGCAGAAGGA CACGCGAGCC
    CAGCTCGTGC TGTGGGACTG CCTCATCCTC TATAATGTCA CTGTCATCAT CTCTAAGAAT
    ATGCTGTCGC TCCTGTCCTG TGTCTTCGTG GAGCAAATGC AGAGCAACTT CTGCTGGGTC
    ATCCAGCTCT TCAGCCTCGT GTGCACAGTC AAAGGCTACT ATGATCCCAA AGAGATGATG
    ACCAGGGACC GGGACTGCCT GCTGCCTGTG GAGGAGGCCG GGATCATCTG GGACAGTATC
    TGCTTCTTCT TCCTGCTCTT GCAACGGCGC ATCTTTCTCA GCCACTACTT CCTGCATGTC
    AGCGCTGACC TGAAAGCCAC AGCCCTGCAG GCATCCAGGG GCTTTGCCCT CTACAATGCA
    GCCAACCTGA AGAGCATCAA CTTCCATCGC CAGATTGAGG AGAAGTCCCT GGCCCAGCTG
    AAAAGACAGA TGAAGCGCAT CCGTGCCAAA CAGGAGAAGT ACAGGCAGAG CCAGGCAAGT
    CGTGGCCAAC TCCAGTCCAA AGACCCTCAG GATCCCAGCC AGGAGCCAGG GCCTGACAGC
    CCAGGGGGCT CCTCCCCGCC ACGGAGACAG TGGTGGCGCC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGCGACTA CTTCCTGTTT GAGTCAGATA GCGAGGAGGA AGAGGAGGCC
    CTACCTGAGG ACCCCAGGCC TGCAGCTCAG AGTGCCTTCC AGATGGCATA CCAGGCATGG
    GTAACCAATG CCCAGACAGT GCTGAGGCAG CGTCGGGAGC GGGCACGGCA GGAGCGGGCA
    GAGCAGCTGG CTTCTGGAGG TGACTTGAAC CCAGATGTGG AACCAGTAGA TGTCCCAGAA
    GATGAGATGG CAGGCCGTAG CCACATGATG CAGCGTGTGC TAAGCACCAT GCAGTTCCTG
    TGGGTGCTGG GCCAGGCCAC GGTAGACGGG CTGACGCGCT GGCTGCGTGC ATTCACGAAG
    CACCACCGCA CCATGAGCGA TGTGCTGTGC GCAGAGCGCT ACCTGCTCAC CCAGGAGCTT
    CTTCGGGTTG GAGAGGTACG CCGAGGTGTG CTGGACCAGC TTTATGTGGG TGAAGATGAG
    GCCACATTGT CAGGTCCCAT GGAGACCCGG GATGGACCCA GCACAGCCTC AAGTGGGCTG
    GGAGCCGAAG AGCCTTTGAG TAGCATGACA GACGACACCA GCAGCCCCCT GAGCACAGGC
    TATAACACCC GCAGTGGCAG TGAGGAGATT GTCACCGACG CTGGGGACCT CCAGGCTGGG
    ACCTCCCTGC ACGGCTCCCA AGAGCTTTTA GCCAATGCTC GTACCCGGAT GCGCACGGCC
    AGCGAGCTGC TACTGGATAG GCGCCTGCAT ATCCCTGAGC TGGAGGAGGC CGAGCGGTTT
    GAGGCACAGC AGGGCCGGAC TCTGCGGCTG CTCAGGGCTG GGTACCAGTG CGTGGCGGCA
    CACTCGGAGC TGCTCTGTTA CTTCATCATC ATCCTTAACC ACATGGTGAC AGCCTCGGCT
    GCCTCCCTGG TGCTGCCCGT GCTTGTGTTC CTGTGGGCCA TGCTGACCAT CCCGAGGCCT
    AGCAAGCGCT TTTGGATGAC AGCTATCGTC TTCACTGAGG TCATGGTGGT CACCAAATAC
    CTGTTCCAGT TCGGCTTCTT CCCCTGGAAC AGCTACGTTG TGCTGCGGCG CTATGAGAAC
    AAGCCCTACT TCCCTCCGCG AATCCTGGGC CTTGAGAAAA CGGACAGCTA CATCAAGTAT
    GACCTGGTGC AGCTCATGGC CCTCTTCTTC CACCGCTCGC AGCTACTGTG TTATGGCCTC
    TGGGACCATG AGGAGGATCG CTATCCCAAG GACCATTGCA GGAGTAGTGT GAAGGACCGG
    GAGGCCAAGG AAGAGCCAGA AGCTAAGCTG GAATCGCAGT CTGAGACAGG CACTGGGCAT
    CCCAAGGAGC CAGTGTTGGC CGGTACTCCC AGGGACCACA TCCAAGGGAA AGGAAGTATT
    AGATCCAAGG ATGTTATCCA AGATCCCCCA GAGGACCTTA AGCCCCGGCA CACGAGGCAC
    ATCAGCATAC GCTTCAGGAG GCGCAAGGAG ACTCCAGGAC CCAAAGGAAC AGCAGTCATG
    GAGACTGAGC ACGAGGAGGG AGAAGGAAAA GAAACTACAG AGAGAAAGAG GCCGCGTCAC
    ACTCAAGAAA AATCGAAGTT TCGGGAGAGA ATGAAGGCAG CTGGGCGCCG GCTGCAGAGC
    TTCTGTGTGT CACTGGCCCA GAGCTTCTAC CAACCCTTGC AGCGTTTCTT CCATGACATT
    CTGCACACAA AGTACCGGGC GGCCACCGAC GTCTACGCCC TCATGTTCCT GGCCGATATT
    GTCGACATCA TCATCATCAT CTTTGGTTTT TGGGCTTTTG GGAAGCACTC TGCAGCCACA
    GACATTGCAT CCTCGCTGTC AGATGACCAG GTGCCACAGG CCTTCCTGTT CATGCTGCTG
    GTCCAGTTTG GCACCATGGT CATCGACCGT GCCCTCTACC TGCGCAAGAC TGTCCTGGGA
    AAGCTGGCCT TTCAGGTGGT CCTGGTGGTG GCGATTCACA TCTGGATGTT CTTTATCTTA
    CCGGCTGTCA CTGAGAGGAT GTTCAGCCAG AATGCGGTGG CACAGCTGTG GTACTTCGTC
    AAGTGCATTT ACTTTGCCCT GTCCGCCTAC CAGATCCGCT GTGGCTACCC CACCCGTATC
    TTGGGCAACT TCCTCACCAA GAAATACAAC CATCTAAACC TCTTCCTTTT CCAGGGGTTC
    CGTCTAGTGC CGTTCCTGGT GGAGCTGCGG GCCGTCATGG ACTGGGTGTG GACCGACACC
    ACGCTGTCCC TGTCCAACTG GATGTGTGTG GAAGACATCT ATGCCAACAT CTTTATCATC
    AAGTGCAGCC GAGAGACAGA GAAGAAATAC CCCCAGCCCA AGGGGCAGAA GAAGAAGAAA
    ATTGTCAAGT ATGGTATGGG AGGCCTCATT ATCCTCTTCC TCATCGCCAT CATCTGGTTC
    CCTCTGCTCT TCATGTCACT GATCCGCTCT GTGGTCGGGG TCGTCAACCA GCCCATTGAT
    GTCACCGTCA CCCTCAAGCT AGGCGGCTAT GAGCCACTGT TCACCATGAG CGCCCAGCAG
    CCATCCATTG TGCCATTCAC ACCCCAGGCC TACGAGGAGC TGTCCCAGCA GTTTGACCCC
    TATCCACTAG CCATGCAGTT CATTAGCCAG TACAGTCCTG AGGACATCGT CACTGCACAG
    ATCGAGGGCA GCTCGGGGGC GCTGTGGCGC ATCAGCCCAC CCAGCCGAGC CCAGATGAAG
    CAGGAGCTGT ACAACGGCAC AGCCGACATT ACACTGCGCT TTACCTGGAA TTTCCAAAGG
    GACCTGGCCA AGGGTGGCAC TGTGGAGTAT ACTAATGAGA AGCACACCTT GGAGCTGGCC
    CCCAACAGTA CGGCACGAAG GCAGCTGGCC CAACTGCTCG AGGGCAGACC TGACCAGTCA
    GTGGTCATTC CCCATCTCTT CCCCAAGTAC ATTCGTGCTC CCAATGGGCC TGAAGCCAAC
    CCTGTGAAGC AGCTGCAGCC AGATGAGGAA GAGGACTACC TTGGTGTGCG CATCCAGCTG
    CGGAGGGAGC AAGTGGGCAC AGGGGCCTCT GGGGAGCAAG CGGGCACCAA GGCCTCCGAC
    TTCCTCGAGT GGTGGGTCAT CGAGCTGCAG GACTGCAAGG CTGACTGCAA CCTGCTGCCC
    ATGGTCATCT TCAGTGACAA GGTCAGCCCA CCTAGCCTGG GCTTCCTGGC CGGCTACGGG
    ATTGTGGGGC TGTACGTCTC CATCGTGCTG GTGGTTGGCA AGTTTGTGCG GGGCTTCTTC
    AGCGAGATCT CTCACTCCAT CATGTTCGAG GAACTGCCGT GTGTGGACCG CATCCTCAAG
    CTGTGCCAGG ACATCTTCTT GGTGCGCGAG ACCCGGGAGC TGGAGCTGGA GGAGGAGCTA
    TACGCCAAGC TCATCTTCCT GTACCGATCT CCAGAGACCA TGATTAAGTG GACACGTGAG
    AGGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu18271
    Clone ID Related Accession (Same CDS sequence) NM_001037298.1
    Accession Version NM_001037298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114917.2. On Sep 9, 2009 this sequence version replaced XM_001481316.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTGCCCTG CACGCTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGCTGGTCT ATTTGCTGTT TCTACTGCTG
    CTGCCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTC
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ACCTTGCCTT TCAGATATGC
    CTACACACCG TGCCTCACCT GGACCAGTTT CTGGGACAAA ACGGTAGCCT TTGGGTGAAG
    GTGTCTCAAC ACATAGGGGT TACGAGGCTG GACCTGAAGG ACATCTTTAA CACCACCAGG
    CTGGTAGCAC CTGACCTGGG AGTGCTGCTG GCGTCCTCCC TTTGCCTTGG CCTCTGTGGA
    CGCCTCACGA GGAAAGCCCG GCAGAGTCGG CGCACCCAGG AGCTGGATGA TGACGATGAC
    GATGATGACG ACGATGAAGA CATAGATGCT GCCCCAGCCG TGGGGCTGAA GGGAGCCCCT
    GCCCTGGCAA CCAAACGCAG GCTGTGGCTG GCCTCCCGCT TCCGGGTCAC GGCCCACTGG
    CTGCTGATGA CCTCTGGACG GACGCTGGTC ATTGTGTTGC TGGCCCTGGC AGGCATAGCC
    CACCCTTCGG CCTTCTCCAG CGTCTACCTG GTGGTGTTCC TGGCCATCTG CACCTGGTGG
    TCCTGTCACT TTCCTCTCAG CCCCCTGGGC TTTAACACCC TCTGTGTCAT GGTGAGCTGT
    TTTGGTGCCG GCCATCTCAT TTGCCTATAC TGCTATCAGA CACCATTTAT CCAGGACATG
    TTACCCCCTG GGAACATCTG GGCCAGGCTA TTTGGTCTCA AGAACTTCGT AGACCTCCCT
    AACTACTCCA GCCCCAACGC CCTGGTACTC AACACTAAGC ACGCCTGGCC CATCTATGTG
    AGTCCCGGAA TCCTGCTGCT GCTATATTAC ACAGCCACCT CTCTCCTGAA GCTCCACAAG
    AGCTGTCCCT CAGAGCTGAG GAAGGAGACA CCCAGGGAGG ATGAGGAGCA TGAGCTGGAA
    CTGGACCACC TGGAGCCAGA GCCCCAGGCT AGGGACGCCA CCCAGGGTGA GATGCCCATG
    ACCACGGAAC CTGACCTTGA CAACTGCACC GTGCATGTAC TAACCAGCCA GAGCCCTGTC
    CGCCAGCGTC CAGTTCGCCC CAGGCTGGCT GAGCTGAAAG AGATGTCACC GCTACATGGC
    CTGGGCCACC TCATCATGGA CCAGAGCTAT GTGTGTGCCC TGATTGCCAT GATGGTGTGG
    AGCATCATGT ACCACAGCTG GCTGACCTTC GTCCTGCTGC TCTGGGCCTG CCTCATCTGG
    ACGGTGCGTA GCCGCCACCA GCTGGCTATG CTCTGCTCGC CCTGCATCCT GCTGTATGGG
    CTGACGCTCT GCTGCCTGAG GTACGTGTGG GCCATGGAAC TTCCTGAGCT GCCCACCACC
    CTGGGCCCTG TCAGCCTGCA CCAGTTGGGA CTGGAACACA CACGCTACCC TTGCCTGGAC
    CTCGGTGCCA TGCTCCTCTA TCTGCTCACA TTCTGGCTCC TTCTGCGTCA GTTCGTGAAG
    GAGAAGCTGC TGAAGAAGCA AAAGGTGCCT GCGGCGCTTT TGGAGGTCAC GGTGGCCGAC
    ACTGAGCCCA CACAGACCCA GACGCTGCTG CGGAGCCTGG GGGAGCTGGT CACCGGCATC
    TACGTCAAAT ACTGGATCTA TGTGTGCGCC GGCATGTTCA TTGTGGTCAG CTTCGCCGGC
    CGCCTGGTGG TCTACAAAAT CGTCTACATG TTCCTCTTCC TGCTGTGCCT CACCTTGTTC
    CAGGTCTACT ACACCCTGTG GAGGAAGCTG CTGCGTGTCT TCTGGTGGCT CGTGGTGGCC
    TATACAATGC TCGTGCTCAT CGCTGTGTAC ACCTTCCAGT TCCAGGACTT CCCCACCTAT
    TGGCGCAACC TCACGGGCTT CACGGACGAG CAGTTGGGCG ACCTGGGCCT GGAGCAGTTC
    AGTGTGTCGG AGCTCTTTTC CAGTATCCTC ATCCCTGGCT TCTTCCTGCT GGCCTGCATC
    CTGCAGCTGC ACTACTTCCA CAGACCGTTC ATGCAGCTCA CTGACCTGGA GCACGTGCCG
    CCACCAGGCA CCCGCCACCC TCGATGGGCT CACAGGCAGG ATGCAGTGAG CGAGGCCCCT
    CTGCTTGAGC ATCAGGAGGA AGAGGAAGTC TTCAGGGAAG ATGGGCAGAG CATGGATGGG
    CCCCACCAGG CCACACAGGT CCCTGAGGGT ACGGCCAGCA AGTGGGGCCT GGTGGCTGAC
    CGGCTGCTGG ACCTAGCGGC CAGCTTCTCG GCTGTCCTCA CCCGAATCCA GGTGTTCGTT
    CGGCGCTTGC TAGAACTTCA CGTCTTCAAG CTGGTGGCCC TCTACACTGT CTGGGTGGCC
    CTGAAAGAAG TGTCTGTGAT GAACCTGCTG CTGGTGGTGC TATGGGCCTT CGCCTTGCCC
    TATCCGCGCT TCCGGCCCAT GGCTTCCTGC CTGTCCACCG TGTGGACCTG TATCATCATT
    GTATGCAAGA TGCTCTATCA GCTCAAGATT GTCAACCCGC ATGAGTACTC CAGCAACTGC
    ACTGAGCCCT TCCCCAACAA TACCAACTTG CAGCCTTTGG AGATCAACCA GTCTTTGCTG
    TACCGTGGCC CTGTTGACCC TGCCAACTGG TTTGGGGTGC GGAAGGGTTA CCCCAACTTG
    GGCTATATCC AGAACCACCT GCAGATCCTT CTGTTGCTGG TGTTTGAGGC CGTGGTGTAC
    CGGCGCCAAG AGCACTACCG CCGGCAGCAC CAGCAGGCCC CTCTGCCCGC CCAGGCTGTG
    TGCGCAGATG GCACCCGCCA GAGGTTGGAC CAGGACCTAC TTAGCTGCCT CAAGTATTTC
    ATCAACTTCT TCTTCTACAA ATTCGGGCTG GAGATCTGCT TCTTGATGGC CGTGAATGTG
    ATTGGGCAGC GTATGAACTT CATGGTGATC TTACACGGTT GCTGGTTGGT GGCCATCCTT
    ACACGCCGGC GCCGTGAGGC CATCGCCCGC CTCTGGCCTA ACTACTGTCT GTTCCTCACG
    CTGTTCCTGC TGTACCAGTA CCTGCTGTGT TTGGGCATGC CCCCTGCACT GTGCATTGAC
    TATCCATGGC GCTGGAGCAA GGCCATCCCC ATGAATTCCG CCCTCATCAA GTGGCTGTAC
    CTACCTGACT TCTTCAGAGC CCCCAACTCC ACCAACCTTA TCAGTGACTT CCTCCTGCTG
    CTTTGCGCCT CCCAGCAGTG GCAGGTCTTC TCAGCGGAGC GAACGGAGGA GTGGCAACGC
    ATGGCGGGCA TCAACACTGA CCACCTGGAG CCCCTGCGTG GGGAGCCCAA CCCTATACCC
    AACTTCATCC ACTGCAGGTC CTATCTGGAT ATGCTGAAGG TGGCCGTCTT CCGCTACCTG
    TTCTGGCTGG TGCTCGTTGT GGTGTTTGTT GCGGGGGCCA CCCGCATAAG CATCTTCGGG
    CTGGGGTACC TGCTAGCCTG CTTCTACCTG CTGCTGTTTG GCACTACCCT GCTGCAGAAG
    GACACGCGAG CCCAGCTCGT GCTGTGGGAC TGCCTCATCC TCTATAATGT CACTGTCATC
    ATCTCTAAGA ATATGCTGTC GCTCCTGTCC TGTGTCTTCG TGGAGCAAAT GCAGAGCAAC
    TTCTGCTGGG TCATCCAGCT CTTCAGCCTC GTGTGCACAG TCAAAGGCTA CTATGATCCC
    AAAGAGATGA TGACCAGGGA CCGGGACTGC CTGCTGCCTG TGGAGGAGGC CGGGATCATC
    TGGGACAGTA TCTGCTTCTT CTTCCTGCTC TTGCAACGGC GCATCTTTCT CAGCCACTAC
    TTCCTGCATG TCAGCGCTGA CCTGAAAGCC ACAGCCCTGC AGGCATCCAG GGGCTTTGCC
    CTCTACAATG CAGCCAACCT GAAGAGCATC AACTTCCATC GCCAGATTGA GGAGAAGTCC
    CTGGCCCAGC TGAAAAGACA GATGAAGCGC ATCCGTGCCA AACAGGAGAA GTACAGGCAG
    AGCCAGGCAA GTCGTGGCCA ACTCCAGTCC AAAGACCCTC AGGATCCCAG CCAGGAGCCA
    GGGCCTGACA GCCCAGGGGG CTCCTCCCCG CCACGGAGAC AGTGGTGGCG CCCCTGGCTG
    GACCACGCCA CAGTCATCCA CTCTGGCGAC TACTTCCTGT TTGAGTCAGA TAGCGAGGAG
    GAAGAGGAGG CCCTACCTGA GGACCCCAGG CCTGCAGCTC AGAGTGCCTT CCAGATGGCA
    TACCAGGCAT GGGTAACCAA TGCCCAGACA GTGCTGAGGC AGCGTCGGGA GCGGGCACGG
    CAGGAGCGGG CAGAGCAGCT GGCTTCTGGA GGTGACTTGA ACCCAGATGT GGAACCAGTA
    GATGTCCCAG AAGATGAGAT GGCAGGCCGT AGCCACATGA TGCAGCGTGT GCTAAGCACC
    ATGCAGTTCC TGTGGGTGCT GGGCCAGGCC ACGGTAGACG GGCTGACGCG CTGGCTGCGT
    GCATTCACGA AGCACCACCG CACCATGAGC GATGTGCTGT GCGCAGAGCG CTACCTGCTC
    ACCCAGGAGC TTCTTCGGGT TGGAGAGGTA CGCCGAGGTG TGCTGGACCA GCTTTATGTG
    GGTGAAGATG AGGCCACATT GTCAGGTCCC ATGGAGACCC GGGATGGACC CAGCACAGCC
    TCAAGTGGGC TGGGAGCCGA AGAGCCTTTG AGTAGCATGA CAGACGACAC CAGCAGCCCC
    CTGAGCACAG GCTATAACAC CCGCAGTGGC AGTGAGGAGA TTGTCACCGA CGCTGGGGAC
    CTCCAGGCTG GGACCTCCCT GCACGGCTCC CAAGAGCTTT TAGCCAATGC TCGTACCCGG
    ATGCGCACGG CCAGCGAGCT GCTACTGGAT AGGCGCCTGC ATATCCCTGA GCTGGAGGAG
    GCCGAGCGGT TTGAGGCACA GCAGGGCCGG ACTCTGCGGC TGCTCAGGGC TGGGTACCAG
    TGCGTGGCGG CACACTCGGA GCTGCTCTGT TACTTCATCA TCATCCTTAA CCACATGGTG
    ACAGCCTCGG CTGCCTCCCT GGTGCTGCCC GTGCTTGTGT TCCTGTGGGC CATGCTGACC
    ATCCCGAGGC CTAGCAAGCG CTTTTGGATG ACAGCTATCG TCTTCACTGA GGTCATGGTG
    GTCACCAAAT ACCTGTTCCA GTTCGGCTTC TTCCCCTGGA ACAGCTACGT TGTGCTGCGG
    CGCTATGAGA ACAAGCCCTA CTTCCCTCCG CGAATCCTGG GCCTTGAGAA AACGGACAGC
    TACATCAAGT ATGACCTGGT GCAGCTCATG GCCCTCTTCT TCCACCGCTC GCAGCTACTG
    TGTTATGGCC TCTGGGACCA TGAGGAGGAT CGCTATCCCA AGGACCATTG CAGGAGTAGT
    GTGAAGGACC GGGAGGCCAA GGAAGAGCCA GAAGCTAAGC TGGAATCGCA GTCTGAGACA
    GGCACTGGGC ATCCCAAGGA GCCAGTGTTG GCCGGTACTC CCAGGGACCA CATCCAAGGG
    AAAGGAAGTA TTAGATCCAA GGATGTTATC CAAGATCCCC CAGAGGACCT TAAGCCCCGG
    CACACGAGGC ACATCAGCAT ACGCTTCAGG AGGCGCAAGG AGACTCCAGG ACCCAAAGGA
    ACAGCAGTCA TGGAGACTGA GCACGAGGAG GGAGAAGGAA AAGAAACTAC AGAGAGAAAG
    AGGCCGCGTC ACACTCAAGA AAAATCGAAG TTTCGGGAGA GAATGAAGGC AGCTGGGCGC
    CGGCTGCAGA GCTTCTGTGT GTCACTGGCC CAGAGCTTCT ACCAACCCTT GCAGCGTTTC
    TTCCATGACA TTCTGCACAC AAAGTACCGG GCGGCCACCG ACGTCTACGC CCTCATGTTC
    CTGGCCGATA TTGTCGACAT CATCATCATC ATCTTTGGTT TTTGGGCTTT TGGGAAGCAC
    TCTGCAGCCA CAGACATTGC ATCCTCGCTG TCAGATGACC AGGTGCCACA GGCCTTCCTG
    TTCATGCTGC TGGTCCAGTT TGGCACCATG GTCATCGACC GTGCCCTCTA CCTGCGCAAG
    ACTGTCCTGG GAAAGCTGGC CTTTCAGGTG GTCCTGGTGG TGGCGATTCA CATCTGGATG
    TTCTTTATCT TACCGGCTGT CACTGAGAGG ATGTTCAGCC AGAATGCGGT GGCACAGCTG
    TGGTACTTCG TCAAGTGCAT TTACTTTGCC CTGTCCGCCT ACCAGATCCG CTGTGGCTAC
    CCCACCCGTA TCTTGGGCAA CTTCCTCACC AAGAAATACA ACCATCTAAA CCTCTTCCTT
    TTCCAGGGGT TCCGTCTAGT GCCGTTCCTG GTGGAGCTGC GGGCCGTCAT GGACTGGGTG
    TGGACCGACA CCACGCTGTC CCTGTCCAAC TGGATGTGTG TGGAAGACAT CTATGCCAAC
    ATCTTTATCA TCAAGTGCAG CCGAGAGACA GAGAAGAAAT ACCCCCAGCC CAAGGGGCAG
    AAGAAGAAGA AAATTGTCAA GTATGGTATG GGAGGCCTCA TTATCCTCTT CCTCATCGCC
    ATCATCTGGT TCCCTCTGCT CTTCATGTCA CTGATCCGCT CTGTGGTCGG GGTCGTCAAC
    CAGCCCATTG ATGTCACCGT CACCCTCAAG CTAGGCGGCT ATGAGCCACT GTTCACCATG
    AGCGCCCAGC AGCCATCCAT TGTGCCATTC ACACCCCAGG CCTACGAGGA GCTGTCCCAG
    CAGTTTGACC CCTATCCACT AGCCATGCAG TTCATTAGCC AGTACAGTCC TGAGGACATC
    GTCACTGCAC AGATCGAGGG CAGCTCGGGG GCGCTGTGGC GCATCAGCCC ACCCAGCCGA
    GCCCAGATGA AGCAGGAGCT GTACAACGGC ACAGCCGACA TTACACTGCG CTTTACCTGG
    AATTTCCAAA GGGACCTGGC CAAGGGTGGC ACTGTGGAGT ATACTAATGA GAAGCACACC
    TTGGAGCTGG CCCCCAACAG TACGGCACGA AGGCAGCTGG CCCAACTGCT CGAGGGCAGA
    CCTGACCAGT CAGTGGTCAT TCCCCATCTC TTCCCCAAGT ACATTCGTGC TCCCAATGGG
    CCTGAAGCCA ACCCTGTGAA GCAGCTGCAG CCAGATGAGG AAGAGGACTA CCTTGGTGTG
    CGCATCCAGC TGCGGAGGGA GCAAGTGGGC ACAGGGGCCT CTGGGGAGCA AGCGGGCACC
    AAGGCCTCCG ACTTCCTCGA GTGGTGGGTC ATCGAGCTGC AGGACTGCAA GGCTGACTGC
    AACCTGCTGC CCATGGTCAT CTTCAGTGAC AAGGTCAGCC CACCTAGCCT GGGCTTCCTG
    GCCGGCTACG GGATTGTGGG GCTGTACGTC TCCATCGTGC TGGTGGTTGG CAAGTTTGTG
    CGGGGCTTCT TCAGCGAGAT CTCTCACTCC ATCATGTTCG AGGAACTGCC GTGTGTGGAC
    CGCATCCTCA AGCTGTGCCA GGACATCTTC TTGGTGCGCG AGACCCGGGA GCTGGAGCTG
    GAGGAGGAGC TATACGCCAA GCTCATCTTC CTGTACCGAT CTCCAGAGAC CATGATTAAG
    TGGACACGTG AGAGGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001032375.1
    CDS292..7932
    Translation

    Target ORF information:

    RefSeq Version NM_001037298.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001037298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTGCCCTG CACGCTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGCTGGTCT ATTTGCTGTT TCTACTGCTG
    CTGCCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTC
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ACCTTGCCTT TCAGATATGC
    CTACACACCG TGCCTCACCT GGACCAGTTT CTGGGACAAA ACGGTAGCCT TTGGGTGAAG
    GTGTCTCAAC ACATAGGGGT TACGAGGCTG GACCTGAAGG ACATCTTTAA CACCACCAGG
    CTGGTAGCAC CTGACCTGGG AGTGCTGCTG GCGTCCTCCC TTTGCCTTGG CCTCTGTGGA
    CGCCTCACGA GGAAAGCCCG GCAGAGTCGG CGCACCCAGG AGCTGGATGA TGACGATGAC
    GATGATGACG ACGATGAAGA CATAGATGCT GCCCCAGCCG TGGGGCTGAA GGGAGCCCCT
    GCCCTGGCAA CCAAACGCAG GCTGTGGCTG GCCTCCCGCT TCCGGGTCAC GGCCCACTGG
    CTGCTGATGA CCTCTGGACG GACGCTGGTC ATTGTGTTGC TGGCCCTGGC AGGCATAGCC
    CACCCTTCGG CCTTCTCCAG CGTCTACCTG GTGGTGTTCC TGGCCATCTG CACCTGGTGG
    TCCTGTCACT TTCCTCTCAG CCCCCTGGGC TTTAACACCC TCTGTGTCAT GGTGAGCTGT
    TTTGGTGCCG GCCATCTCAT TTGCCTATAC TGCTATCAGA CACCATTTAT CCAGGACATG
    TTACCCCCTG GGAACATCTG GGCCAGGCTA TTTGGTCTCA AGAACTTCGT AGACCTCCCT
    AACTACTCCA GCCCCAACGC CCTGGTACTC AACACTAAGC ACGCCTGGCC CATCTATGTG
    AGTCCCGGAA TCCTGCTGCT GCTATATTAC ACAGCCACCT CTCTCCTGAA GCTCCACAAG
    AGCTGTCCCT CAGAGCTGAG GAAGGAGACA CCCAGGGAGG ATGAGGAGCA TGAGCTGGAA
    CTGGACCACC TGGAGCCAGA GCCCCAGGCT AGGGACGCCA CCCAGGGTGA GATGCCCATG
    ACCACGGAAC CTGACCTTGA CAACTGCACC GTGCATGTAC TAACCAGCCA GAGCCCTGTC
    CGCCAGCGTC CAGTTCGCCC CAGGCTGGCT GAGCTGAAAG AGATGTCACC GCTACATGGC
    CTGGGCCACC TCATCATGGA CCAGAGCTAT GTGTGTGCCC TGATTGCCAT GATGGTGTGG
    AGCATCATGT ACCACAGCTG GCTGACCTTC GTCCTGCTGC TCTGGGCCTG CCTCATCTGG
    ACGGTGCGTA GCCGCCACCA GCTGGCTATG CTCTGCTCGC CCTGCATCCT GCTGTATGGG
    CTGACGCTCT GCTGCCTGAG GTACGTGTGG GCCATGGAAC TTCCTGAGCT GCCCACCACC
    CTGGGCCCTG TCAGCCTGCA CCAGTTGGGA CTGGAACACA CACGCTACCC TTGCCTGGAC
    CTCGGTGCCA TGCTCCTCTA TCTGCTCACA TTCTGGCTCC TTCTGCGTCA GTTCGTGAAG
    GAGAAGCTGC TGAAGAAGCA AAAGGTGCCT GCGGCGCTTT TGGAGGTCAC GGTGGCCGAC
    ACTGAGCCCA CACAGACCCA GACGCTGCTG CGGAGCCTGG GGGAGCTGGT CACCGGCATC
    TACGTCAAAT ACTGGATCTA TGTGTGCGCC GGCATGTTCA TTGTGGTCAG CTTCGCCGGC
    CGCCTGGTGG TCTACAAAAT CGTCTACATG TTCCTCTTCC TGCTGTGCCT CACCTTGTTC
    CAGGTCTACT ACACCCTGTG GAGGAAGCTG CTGCGTGTCT TCTGGTGGCT CGTGGTGGCC
    TATACAATGC TCGTGCTCAT CGCTGTGTAC ACCTTCCAGT TCCAGGACTT CCCCACCTAT
    TGGCGCAACC TCACGGGCTT CACGGACGAG CAGTTGGGCG ACCTGGGCCT GGAGCAGTTC
    AGTGTGTCGG AGCTCTTTTC CAGTATCCTC ATCCCTGGCT TCTTCCTGCT GGCCTGCATC
    CTGCAGCTGC ACTACTTCCA CAGACCGTTC ATGCAGCTCA CTGACCTGGA GCACGTGCCG
    CCACCAGGCA CCCGCCACCC TCGATGGGCT CACAGGCAGG ATGCAGTGAG CGAGGCCCCT
    CTGCTTGAGC ATCAGGAGGA AGAGGAAGTC TTCAGGGAAG ATGGGCAGAG CATGGATGGG
    CCCCACCAGG CCACACAGGT CCCTGAGGGT ACGGCCAGCA AGTGGGGCCT GGTGGCTGAC
    CGGCTGCTGG ACCTAGCGGC CAGCTTCTCG GCTGTCCTCA CCCGAATCCA GGTGTTCGTT
    CGGCGCTTGC TAGAACTTCA CGTCTTCAAG CTGGTGGCCC TCTACACTGT CTGGGTGGCC
    CTGAAAGAAG TGTCTGTGAT GAACCTGCTG CTGGTGGTGC TATGGGCCTT CGCCTTGCCC
    TATCCGCGCT TCCGGCCCAT GGCTTCCTGC CTGTCCACCG TGTGGACCTG TATCATCATT
    GTATGCAAGA TGCTCTATCA GCTCAAGATT GTCAACCCGC ATGAGTACTC CAGCAACTGC
    ACTGAGCCCT TCCCCAACAA TACCAACTTG CAGCCTTTGG AGATCAACCA GTCTTTGCTG
    TACCGTGGCC CTGTTGACCC TGCCAACTGG TTTGGGGTGC GGAAGGGTTA CCCCAACTTG
    GGCTATATCC AGAACCACCT GCAGATCCTT CTGTTGCTGG TGTTTGAGGC CGTGGTGTAC
    CGGCGCCAAG AGCACTACCG CCGGCAGCAC CAGCAGGCCC CTCTGCCCGC CCAGGCTGTG
    TGCGCAGATG GCACCCGCCA GAGGTTGGAC CAGGACCTAC TTAGCTGCCT CAAGTATTTC
    ATCAACTTCT TCTTCTACAA ATTCGGGCTG GAGATCTGCT TCTTGATGGC CGTGAATGTG
    ATTGGGCAGC GTATGAACTT CATGGTGATC TTACACGGTT GCTGGTTGGT GGCCATCCTT
    ACACGCCGGC GCCGTGAGGC CATCGCCCGC CTCTGGCCTA ACTACTGTCT GTTCCTCACG
    CTGTTCCTGC TGTACCAGTA CCTGCTGTGT TTGGGCATGC CCCCTGCACT GTGCATTGAC
    TATCCATGGC GCTGGAGCAA GGCCATCCCC ATGAATTCCG CCCTCATCAA GTGGCTGTAC
    CTACCTGACT TCTTCAGAGC CCCCAACTCC ACCAACCTTA TCAGTGACTT CCTCCTGCTG
    CTTTGCGCCT CCCAGCAGTG GCAGGTCTTC TCAGCGGAGC GAACGGAGGA GTGGCAACGC
    ATGGCGGGCA TCAACACTGA CCACCTGGAG CCCCTGCGTG GGGAGCCCAA CCCTATACCC
    AACTTCATCC ACTGCAGGTC CTATCTGGAT ATGCTGAAGG TGGCCGTCTT CCGCTACCTG
    TTCTGGCTGG TGCTCGTTGT GGTGTTTGTT GCGGGGGCCA CCCGCATAAG CATCTTCGGG
    CTGGGGTACC TGCTAGCCTG CTTCTACCTG CTGCTGTTTG GCACTACCCT GCTGCAGAAG
    GACACGCGAG CCCAGCTCGT GCTGTGGGAC TGCCTCATCC TCTATAATGT CACTGTCATC
    ATCTCTAAGA ATATGCTGTC GCTCCTGTCC TGTGTCTTCG TGGAGCAAAT GCAGAGCAAC
    TTCTGCTGGG TCATCCAGCT CTTCAGCCTC GTGTGCACAG TCAAAGGCTA CTATGATCCC
    AAAGAGATGA TGACCAGGGA CCGGGACTGC CTGCTGCCTG TGGAGGAGGC CGGGATCATC
    TGGGACAGTA TCTGCTTCTT CTTCCTGCTC TTGCAACGGC GCATCTTTCT CAGCCACTAC
    TTCCTGCATG TCAGCGCTGA CCTGAAAGCC ACAGCCCTGC AGGCATCCAG GGGCTTTGCC
    CTCTACAATG CAGCCAACCT GAAGAGCATC AACTTCCATC GCCAGATTGA GGAGAAGTCC
    CTGGCCCAGC TGAAAAGACA GATGAAGCGC ATCCGTGCCA AACAGGAGAA GTACAGGCAG
    AGCCAGGCAA GTCGTGGCCA ACTCCAGTCC AAAGACCCTC AGGATCCCAG CCAGGAGCCA
    GGGCCTGACA GCCCAGGGGG CTCCTCCCCG CCACGGAGAC AGTGGTGGCG CCCCTGGCTG
    GACCACGCCA CAGTCATCCA CTCTGGCGAC TACTTCCTGT TTGAGTCAGA TAGCGAGGAG
    GAAGAGGAGG CCCTACCTGA GGACCCCAGG CCTGCAGCTC AGAGTGCCTT CCAGATGGCA
    TACCAGGCAT GGGTAACCAA TGCCCAGACA GTGCTGAGGC AGCGTCGGGA GCGGGCACGG
    CAGGAGCGGG CAGAGCAGCT GGCTTCTGGA GGTGACTTGA ACCCAGATGT GGAACCAGTA
    GATGTCCCAG AAGATGAGAT GGCAGGCCGT AGCCACATGA TGCAGCGTGT GCTAAGCACC
    ATGCAGTTCC TGTGGGTGCT GGGCCAGGCC ACGGTAGACG GGCTGACGCG CTGGCTGCGT
    GCATTCACGA AGCACCACCG CACCATGAGC GATGTGCTGT GCGCAGAGCG CTACCTGCTC
    ACCCAGGAGC TTCTTCGGGT TGGAGAGGTA CGCCGAGGTG TGCTGGACCA GCTTTATGTG
    GGTGAAGATG AGGCCACATT GTCAGGTCCC ATGGAGACCC GGGATGGACC CAGCACAGCC
    TCAAGTGGGC TGGGAGCCGA AGAGCCTTTG AGTAGCATGA CAGACGACAC CAGCAGCCCC
    CTGAGCACAG GCTATAACAC CCGCAGTGGC AGTGAGGAGA TTGTCACCGA CGCTGGGGAC
    CTCCAGGCTG GGACCTCCCT GCACGGCTCC CAAGAGCTTT TAGCCAATGC TCGTACCCGG
    ATGCGCACGG CCAGCGAGCT GCTACTGGAT AGGCGCCTGC ATATCCCTGA GCTGGAGGAG
    GCCGAGCGGT TTGAGGCACA GCAGGGCCGG ACTCTGCGGC TGCTCAGGGC TGGGTACCAG
    TGCGTGGCGG CACACTCGGA GCTGCTCTGT TACTTCATCA TCATCCTTAA CCACATGGTG
    ACAGCCTCGG CTGCCTCCCT GGTGCTGCCC GTGCTTGTGT TCCTGTGGGC CATGCTGACC
    ATCCCGAGGC CTAGCAAGCG CTTTTGGATG ACAGCTATCG TCTTCACTGA GGTCATGGTG
    GTCACCAAAT ACCTGTTCCA GTTCGGCTTC TTCCCCTGGA ACAGCTACGT TGTGCTGCGG
    CGCTATGAGA ACAAGCCCTA CTTCCCTCCG CGAATCCTGG GCCTTGAGAA AACGGACAGC
    TACATCAAGT ATGACCTGGT GCAGCTCATG GCCCTCTTCT TCCACCGCTC GCAGCTACTG
    TGTTATGGCC TCTGGGACCA TGAGGAGGAT CGCTATCCCA AGGACCATTG CAGGAGTAGT
    GTGAAGGACC GGGAGGCCAA GGAAGAGCCA GAAGCTAAGC TGGAATCGCA GTCTGAGACA
    GGCACTGGGC ATCCCAAGGA GCCAGTGTTG GCCGGTACTC CCAGGGACCA CATCCAAGGG
    AAAGGAAGTA TTAGATCCAA GGATGTTATC CAAGATCCCC CAGAGGACCT TAAGCCCCGG
    CACACGAGGC ACATCAGCAT ACGCTTCAGG AGGCGCAAGG AGACTCCAGG ACCCAAAGGA
    ACAGCAGTCA TGGAGACTGA GCACGAGGAG GGAGAAGGAA AAGAAACTAC AGAGAGAAAG
    AGGCCGCGTC ACACTCAAGA AAAATCGAAG TTTCGGGAGA GAATGAAGGC AGCTGGGCGC
    CGGCTGCAGA GCTTCTGTGT GTCACTGGCC CAGAGCTTCT ACCAACCCTT GCAGCGTTTC
    TTCCATGACA TTCTGCACAC AAAGTACCGG GCGGCCACCG ACGTCTACGC CCTCATGTTC
    CTGGCCGATA TTGTCGACAT CATCATCATC ATCTTTGGTT TTTGGGCTTT TGGGAAGCAC
    TCTGCAGCCA CAGACATTGC ATCCTCGCTG TCAGATGACC AGGTGCCACA GGCCTTCCTG
    TTCATGCTGC TGGTCCAGTT TGGCACCATG GTCATCGACC GTGCCCTCTA CCTGCGCAAG
    ACTGTCCTGG GAAAGCTGGC CTTTCAGGTG GTCCTGGTGG TGGCGATTCA CATCTGGATG
    TTCTTTATCT TACCGGCTGT CACTGAGAGG ATGTTCAGCC AGAATGCGGT GGCACAGCTG
    TGGTACTTCG TCAAGTGCAT TTACTTTGCC CTGTCCGCCT ACCAGATCCG CTGTGGCTAC
    CCCACCCGTA TCTTGGGCAA CTTCCTCACC AAGAAATACA ACCATCTAAA CCTCTTCCTT
    TTCCAGGGGT TCCGTCTAGT GCCGTTCCTG GTGGAGCTGC GGGCCGTCAT GGACTGGGTG
    TGGACCGACA CCACGCTGTC CCTGTCCAAC TGGATGTGTG TGGAAGACAT CTATGCCAAC
    ATCTTTATCA TCAAGTGCAG CCGAGAGACA GAGAAGAAAT ACCCCCAGCC CAAGGGGCAG
    AAGAAGAAGA AAATTGTCAA GTATGGTATG GGAGGCCTCA TTATCCTCTT CCTCATCGCC
    ATCATCTGGT TCCCTCTGCT CTTCATGTCA CTGATCCGCT CTGTGGTCGG GGTCGTCAAC
    CAGCCCATTG ATGTCACCGT CACCCTCAAG CTAGGCGGCT ATGAGCCACT GTTCACCATG
    AGCGCCCAGC AGCCATCCAT TGTGCCATTC ACACCCCAGG CCTACGAGGA GCTGTCCCAG
    CAGTTTGACC CCTATCCACT AGCCATGCAG TTCATTAGCC AGTACAGTCC TGAGGACATC
    GTCACTGCAC AGATCGAGGG CAGCTCGGGG GCGCTGTGGC GCATCAGCCC ACCCAGCCGA
    GCCCAGATGA AGCAGGAGCT GTACAACGGC ACAGCCGACA TTACACTGCG CTTTACCTGG
    AATTTCCAAA GGGACCTGGC CAAGGGTGGC ACTGTGGAGT ATACTAATGA GAAGCACACC
    TTGGAGCTGG CCCCCAACAG TACGGCACGA AGGCAGCTGG CCCAACTGCT CGAGGGCAGA
    CCTGACCAGT CAGTGGTCAT TCCCCATCTC TTCCCCAAGT ACATTCGTGC TCCCAATGGG
    CCTGAAGCCA ACCCTGTGAA GCAGCTGCAG CCAGATGAGG AAGAGGACTA CCTTGGTGTG
    CGCATCCAGC TGCGGAGGGA GCAAGTGGGC ACAGGGGCCT CTGGGGAGCA AGCGGGCACC
    AAGGCCTCCG ACTTCCTCGA GTGGTGGGTC ATCGAGCTGC AGGACTGCAA GGCTGACTGC
    AACCTGCTGC CCATGGTCAT CTTCAGTGAC AAGGTCAGCC CACCTAGCCT GGGCTTCCTG
    GCCGGCTACG GGATTGTGGG GCTGTACGTC TCCATCGTGC TGGTGGTTGG CAAGTTTGTG
    CGGGGCTTCT TCAGCGAGAT CTCTCACTCC ATCATGTTCG AGGAACTGCC GTGTGTGGAC
    CGCATCCTCA AGCTGTGCCA GGACATCTTC TTGGTGCGCG AGACCCGGGA GCTGGAGCTG
    GAGGAGGAGC TATACGCCAA GCTCATCTTC CTGTACCGAT CTCCAGAGAC CATGATTAAG
    TGGACACGTG AGAGGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu41422
    Clone ID Related Accession (Same CDS sequence) NM_001357349.1 , XM_006530955.2
    Accession Version NM_001357349.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114917.2. On Oct 26, 2017 this sequence version replaced XM_006530955.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HQ215520.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTGCCCTG CACGCTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGCTGGTCT ATTTGCTGTT TCTACTGCTG
    CTGCCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTC
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ACCTTGCCTT TCAGATATGC
    CTACACACCG TGCCTCACCT GGACCAGTTT CTGGGACAAA ACGGTAGCCT TTGGGTGAAG
    GTGTCTCAAC ACATAGGGGT TACGAGGCTG GACCTGAAGG ACATCTTTAA CACCACCAGG
    CTGGTAGCAC CTGACCTGGG AGTGCTGCTG GCGTCCTCCC TTTGCCTTGG CCTCTGTGGA
    CGCCTCACGA GGAAAGCCCG GCAGAGTCGG CGCACCCAGG AGCTGCAGGA TGATGACGAT
    GACGATGATG ACGACGATGA AGACATAGAT GCTGCCCCAG CCGTGGGGCT GAAGGGAGCC
    CCTGCCCTGG CAACCAAACG CAGGCTGTGG CTGGCCTCCC GCTTCCGGGT CACGGCCCAC
    TGGCTGCTGA TGACCTCTGG ACGGACGCTG GTCATTGTGT TGCTGGCCCT GGCAGGCATA
    GCCCACCCTT CGGCCTTCTC CAGCGTCTAC CTGGTGGTGT TCCTGGCCAT CTGCACCTGG
    TGGTCCTGTC ACTTTCCTCT CAGCCCCCTG GGCTTTAACA CCCTCTGTGT CATGGTGAGC
    TGTTTTGGTG CCGGCCATCT CATTTGCCTA TACTGCTATC AGACACCATT TATCCAGGAC
    ATGTTACCCC CTGGGAACAT CTGGGCCAGG CTATTTGGTC TCAAGAACTT CGTAGACCTC
    CCTAACTACT CCAGCCCCAA CGCCCTGGTA CTCAACACTA AGCACGCCTG GCCCATCTAT
    GTGAGTCCCG GAATCCTGCT GCTGCTATAT TACACAGCCA CCTCTCTCCT GAAGCTCCAC
    AAGAGCTGTC CCTCAGAGCT GAGGAAGGAG ACACCCAGGG AGGATGAGGA GCATGAGCTG
    GAACTGGACC ACCTGGAGCC AGAGCCCCAG GCTAGGGACG CCACCCAGGG TGAGATGCCC
    ATGACCACGG AACCTGACCT TGACAACTGC ACCGTGCATG TACTAACCAG CCAGAGCCCT
    GTCCGCCAGC GTCCAGTTCG CCCCAGGCTG GCTGAGCTGA AAGAGATGTC ACCGCTACAT
    GGCCTGGGCC ACCTCATCAT GGACCAGAGC TATGTGTGTG CCCTGATTGC CATGATGGTG
    TGGAGCATCA TGTACCACAG CTGGCTGACC TTCGTCCTGC TGCTCTGGGC CTGCCTCATC
    TGGACGGTGC GTAGCCGCCA CCAGCTGGCT ATGCTCTGCT CGCCCTGCAT CCTGCTGTAT
    GGGCTGACGC TCTGCTGCCT GAGGTACGTG TGGGCCATGG AACTTCCTGA GCTGCCCACC
    ACCCTGGGCC CTGTCAGCCT GCACCAGTTG GGACTGGAAC ACACACGCTA CCCTTGCCTG
    GACCTCGGTG CCATGCTCCT CTATCTGCTC ACATTCTGGC TCCTTCTGCG TCAGTTCGTG
    AAGGAGAAGC TGCTGAAGAA GCAAAAGGTG CCTGCGGCGC TTTTGGAGGT CACGGTGGCC
    GACACTGAGC CCACACAGAC CCAGACGCTG CTGCGGAGCC TGGGGGAGCT GGTCACCGGC
    ATCTACGTCA AATACTGGAT CTATGTGTGC GCCGGCATGT TCATTGTGGT CAGCTTCGCC
    GGCCGCCTGG TGGTCTACAA AATCGTCTAC ATGTTCCTCT TCCTGCTGTG CCTCACCTTG
    TTCCAGGTCT ACTACACCCT GTGGAGGAAG CTGCTGCGTG TCTTCTGGTG GCTCGTGGTG
    GCCTATACAA TGCTCGTGCT CATCGCTGTG TACACCTTCC AGTTCCAGGA CTTCCCCACC
    TATTGGCGCA ACCTCACGGG CTTCACGGAC GAGCAGTTGG GCGACCTGGG CCTGGAGCAG
    TTCAGTGTGT CGGAGCTCTT TTCCAGTATC CTCATCCCTG GCTTCTTCCT GCTGGCCTGC
    ATCCTGCAGC TGCACTACTT CCACAGACCG TTCATGCAGC TCACTGACCT GGAGCACGTG
    CCGCCACCAG GCACCCGCCA CCCTCGATGG GCTCACAGGC AGGATGCAGT GAGCGAGGCC
    CCTCTGCTTG AGCATCAGGA GGAAGAGGAA GTCTTCAGGG AAGATGGGCA GAGCATGGAT
    GGGCCCCACC AGGCCACACA GGTCCCTGAG GGTACGGCCA GCAAGTGGGG CCTGGTGGCT
    GACCGGCTGC TGGACCTAGC GGCCAGCTTC TCGGCTGTCC TCACCCGAAT CCAGGTGTTC
    GTTCGGCGCT TGCTAGAACT TCACGTCTTC AAGCTGGTGG CCCTCTACAC TGTCTGGGTG
    GCCCTGAAAG AAGTGTCTGT GATGAACCTG CTGCTGGTGG TGCTATGGGC CTTCGCCTTG
    CCCTATCCGC GCTTCCGGCC CATGGCTTCC TGCCTGTCCA CCGTGTGGAC CTGTATCATC
    ATTGTATGCA AGATGCTCTA TCAGCTCAAG ATTGTCAACC CGCATGAGTA CTCCAGCAAC
    TGCACTGAGC CCTTCCCCAA CAATACCAAC TTGCAGCCTT TGGAGATCAA CCAGTCTTTG
    CTGTACCGTG GCCCTGTTGA CCCTGCCAAC TGGTTTGGGG TGCGGAAGGG TTACCCCAAC
    TTGGGCTATA TCCAGAACCA CCTGCAGATC CTTCTGTTGC TGGTGTTTGA GGCCGTGGTG
    TACCGGCGCC AAGAGCACTA CCGCCGGCAG CACCAGCAGG CCCCTCTGCC CGCCCAGGCT
    GTGTGCGCAG ATGGCACCCG CCAGAGGTTG GACCAGGACC TACTTAGCTG CCTCAAGTAT
    TTCATCAACT TCTTCTTCTA CAAATTCGGG CTGGAGATCT GCTTCTTGAT GGCCGTGAAT
    GTGATTGGGC AGCGTATGAA CTTCATGGTG ATCTTACACG GTTGCTGGTT GGTGGCCATC
    CTTACACGCC GGCGCCGTGA GGCCATCGCC CGCCTCTGGC CTAACTACTG TCTGTTCCTC
    ACGCTGTTCC TGCTGTACCA GTACCTGCTG TGTTTGGGCA TGCCCCCTGC ACTGTGCATT
    GACTATCCAT GGCGCTGGAG CAAGGCCATC CCCATGAATT CCGCCCTCAT CAAGTGGCTG
    TACCTACCTG ACTTCTTCAG AGCCCCCAAC TCCACCAACC TTATCAGTGA CTTCCTCCTG
    CTGCTTTGCG CCTCCCAGCA GTGGCAGGTC TTCTCAGCGG AGCGAACGGA GGAGTGGCAA
    CGCATGGCGG GCATCAACAC TGACCACCTG GAGCCCCTGC GTGGGGAGCC CAACCCTATA
    CCCAACTTCA TCCACTGCAG GTCCTATCTG GATATGCTGA AGGTGGCCGT CTTCCGCTAC
    CTGTTCTGGC TGGTGCTCGT TGTGGTGTTT GTTGCGGGGG CCACCCGCAT AAGCATCTTC
    GGGCTGGGGT ACCTGCTAGC CTGCTTCTAC CTGCTGCTGT TTGGCACTAC CCTGCTGCAG
    AAGGACACGC GAGCCCAGCT CGTGCTGTGG GACTGCCTCA TCCTCTATAA TGTCACTGTC
    ATCATCTCTA AGAATATGCT GTCGCTCCTG TCCTGTGTCT TCGTGGAGCA AATGCAGAGC
    AACTTCTGCT GGGTCATCCA GCTCTTCAGC CTCGTGTGCA CAGTCAAAGG CTACTATGAT
    CCCAAAGAGA TGATGACCAG GGACCGGGAC TGCCTGCTGC CTGTGGAGGA GGCCGGGATC
    ATCTGGGACA GTATCTGCTT CTTCTTCCTG CTCTTGCAAC GGCGCATCTT TCTCAGCCAC
    TACTTCCTGC ATGTCAGCGC TGACCTGAAA GCCACAGCCC TGCAGGCATC CAGGGGCTTT
    GCCCTCTACA ATGCAGCCAA CCTGAAGAGC ATCAACTTCC ATCGCCAGAT TGAGGAGAAG
    TCCCTGGCCC AGCTGAAAAG ACAGATGAAG CGCATCCGTG CCAAACAGGA GAAGTACAGG
    CAGAGCCAGG CAAGTCGTGG CCAACTCCAG TCCAAAGACC CTCAGGATCC CAGCCAGGAG
    CCAGGGCCTG ACAGCCCAGG GGGCTCCTCC CCGCCACGGA GACAGTGGTG GCGCCCCTGG
    CTGGACCACG CCACAGTCAT CCACTCTGGC GACTACTTCC TGTTTGAGTC AGATAGCGAG
    GAGGAAGAGG AGGCCCTACC TGAGGACCCC AGGCCTGCAG CTCAGAGTGC CTTCCAGATG
    GCATACCAGG CATGGGTAAC CAATGCCCAG ACAGTGCTGA GGCAGCGTCG GGAGCGGGCA
    CGGCAGGAGC GGGCAGAGCA GCTGGCTTCT GGAGGTGACT TGAACCCAGA TGTGGAACCA
    GTAGATGTCC CAGAAGATGA GATGGCAGGC CGTAGCCACA TGATGCAGCG TGTGCTAAGC
    ACCATGCAGT TCCTGTGGGT GCTGGGCCAG GCCACGGTAG ACGGGCTGAC GCGCTGGCTG
    CGTGCATTCA CGAAGCACCA CCGCACCATG AGCGATGTGC TGTGCGCAGA GCGCTACCTG
    CTCACCCAGG AGCTTCTTCG GGTTGGAGAG GTACGCCGAG GTGTGCTGGA CCAGCTTTAT
    GTGGGTGAAG ATGAGGCCAC ATTGTCAGGT CCCATGGAGA CCCGGGATGG ACCCAGCACA
    GCCTCAAGTG GGCTGGGAGC CGAAGAGCCT TTGAGTAGCA TGACAGACGA CACCAGCAGC
    CCCCTGAGCA CAGGCTATAA CACCCGCAGT GGCAGTGAGG AGATTGTCAC CGACGCTGGG
    GACCTCCAGG CTGGGACCTC CCTGCACGGC TCCCAAGAGC TTTTAGCCAA TGCTCGTACC
    CGGATGCGCA CGGCCAGCGA GCTGCTACTG GATAGGCGCC TGCATATCCC TGAGCTGGAG
    GAGGCCGAGC GGTTTGAGGC ACAGCAGGGC CGGACTCTGC GGCTGCTCAG GGCTGGGTAC
    CAGTGCGTGG CGGCACACTC GGAGCTGCTC TGTTACTTCA TCATCATCCT TAACCACATG
    GTGACAGCCT CGGCTGCCTC CCTGGTGCTG CCCGTGCTTG TGTTCCTGTG GGCCATGCTG
    ACCATCCCGA GGCCTAGCAA GCGCTTTTGG ATGACAGCTA TCGTCTTCAC TGAGGTCATG
    GTGGTCACCA AATACCTGTT CCAGTTCGGC TTCTTCCCCT GGAACAGCTA CGTTGTGCTG
    CGGCGCTATG AGAACAAGCC CTACTTCCCT CCGCGAATCC TGGGCCTTGA GAAAACGGAC
    AGCTACATCA AGTATGACCT GGTGCAGCTC ATGGCCCTCT TCTTCCACCG CTCGCAGCTA
    CTGTGTTATG GCCTCTGGGA CCATGAGGAG GATCGCTATC CCAAGGACCA TTGCAGGAGT
    AGTGTGAAGG ACCGGGAGGC CAAGGAAGAG CCAGAAGCTA AGCTGGAATC GCAGTCTGAG
    ACAGGCACTG GGCATCCCAA GGAGCCAGTG TTGGCCGGTA CTCCCAGGGA CCACATCCAA
    GGGAAAGGAA GTATTAGATC CAAGGATGTT ATCCAAGATC CCCCAGAGGA CCTTAAGCCC
    CGGCACACGA GGCACATCAG CATACGCTTC AGGAGGCGCA AGGAGACTCC AGGACCCAAA
    GGAACAGCAG TCATGGAGAC TGAGCACGAG GAGGGAGAAG GAAAAGAAAC TACAGAGAGA
    AAGAGGCCGC GTCACACTCA AGAAAAATCG AAGTTTCGGG AGAGAATGAA GGCAGCTGGG
    CGCCGGCTGC AGAGCTTCTG TGTGTCACTG GCCCAGAGCT TCTACCAACC CTTGCAGCGT
    TTCTTCCATG ACATTCTGCA CACAAAGTAC CGGGCGGCCA CCGACGTCTA CGCCCTCATG
    TTCCTGGCCG ATATTGTCGA CATCATCATC ATCATCTTTG GTTTTTGGGC TTTTGGGAAG
    CACTCTGCAG CCACAGACAT TGCATCCTCG CTGTCAGATG ACCAGGTGCC ACAGGCCTTC
    CTGTTCATGC TGCTGGTCCA GTTTGGCACC ATGGTCATCG ACCGTGCCCT CTACCTGCGC
    AAGACTGTCC TGGGAAAGCT GGCCTTTCAG GTGGTCCTGG TGGTGGCGAT TCACATCTGG
    ATGTTCTTTA TCTTACCGGC TGTCACTGAG AGGATGTTCA GCCAGAATGC GGTGGCACAG
    CTGTGGTACT TCGTCAAGTG CATTTACTTT GCCCTGTCCG CCTACCAGAT CCGCTGTGGC
    TACCCCACCC GTATCTTGGG CAACTTCCTC ACCAAGAAAT ACAACCATCT AAACCTCTTC
    CTTTTCCAGG GGTTCCGTCT AGTGCCGTTC CTGGTGGAGC TGCGGGCCGT CATGGACTGG
    GTGTGGACCG ACACCACGCT GTCCCTGTCC AACTGGATGT GTGTGGAAGA CATCTATGCC
    AACATCTTTA TCATCAAGTG CAGCCGAGAG ACAGAGAAGA AATACCCCCA GCCCAAGGGG
    CAGAAGAAGA AGAAAATTGT CAAGTATGGT ATGGGAGGCC TCATTATCCT CTTCCTCATC
    GCCATCATCT GGTTCCCTCT GCTCTTCATG TCACTGATCC GCTCTGTGGT CGGGGTCGTC
    AACCAGCCCA TTGATGTCAC CGTCACCCTC AAGCTAGGCG GCTATGAGCC ACTGTTCACC
    ATGAGCGCCC AGCAGCCATC CATTGTGCCA TTCACACCCC AGGCCTACGA GGAGCTGTCC
    CAGCAGTTTG ACCCCTATCC ACTAGCCATG CAGTTCATTA GCCAGTACAG TCCTGAGGAC
    ATCGTCACTG CACAGATCGA GGGCAGCTCG GGGGCGCTGT GGCGCATCAG CCCACCCAGC
    CGAGCCCAGA TGAAGCAGGA GCTGTACAAC GGCACAGCCG ACATTACACT GCGCTTTACC
    TGGAATTTCC AAAGGGACCT GGCCAAGGGT GGCACTGTGG AGTATACTAA TGAGAAGCAC
    ACCTTGGAGC TGGCCCCCAA CAGTACGGCA CGAAGGCAGC TGGCCCAACT GCTCGAGGGC
    AGACCTGACC AGTCAGTGGT CATTCCCCAT CTCTTCCCCA AGTACATTCG TGCTCCCAAT
    GGGCCTGAAG CCAACCCTGT GAAGCAGCTG CAGCCAGATG AGGAAGAGGA CTACCTTGGT
    GTGCGCATCC AGCTGCGGAG GGAGCAAGTG GGCACAGGGG CCTCTGGGGA GCAAGCGGGC
    ACCAAGGCCT CCGACTTCCT CGAGTGGTGG GTCATCGAGC TGCAGGACTG CAAGGCTGAC
    TGCAACCTGC TGCCCATGGT CATCTTCAGT GACAAGGTCA GCCCACCTAG CCTGGGCTTC
    CTGGCCGGCT ACGGGATTGT GGGGCTGTAC GTCTCCATCG TGCTGGTGGT TGGCAAGTTT
    GTGCGGGGCT TCTTCAGCGA GATCTCTCAC TCCATCATGT TCGAGGAACT GCCGTGTGTG
    GACCGCATCC TCAAGCTGTG CCAGGACATC TTCTTGGTGC GCGAGACCCG GGAGCTGGAG
    CTGGAGGAGG AGCTATACGC CAAGCTCATC TTCCTGTACC GATCTCCAGA GACCATGATT
    AAGTGGACAC GTGAGAGGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001344278.1
    CDS292..7935
    Translation

    Target ORF information:

    RefSeq Version NM_001357349.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001357349.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTGCCCTG CACGCTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGCTGGTCT ATTTGCTGTT TCTACTGCTG
    CTGCCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTC
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ACCTTGCCTT TCAGATATGC
    CTACACACCG TGCCTCACCT GGACCAGTTT CTGGGACAAA ACGGTAGCCT TTGGGTGAAG
    GTGTCTCAAC ACATAGGGGT TACGAGGCTG GACCTGAAGG ACATCTTTAA CACCACCAGG
    CTGGTAGCAC CTGACCTGGG AGTGCTGCTG GCGTCCTCCC TTTGCCTTGG CCTCTGTGGA
    CGCCTCACGA GGAAAGCCCG GCAGAGTCGG CGCACCCAGG AGCTGCAGGA TGATGACGAT
    GACGATGATG ACGACGATGA AGACATAGAT GCTGCCCCAG CCGTGGGGCT GAAGGGAGCC
    CCTGCCCTGG CAACCAAACG CAGGCTGTGG CTGGCCTCCC GCTTCCGGGT CACGGCCCAC
    TGGCTGCTGA TGACCTCTGG ACGGACGCTG GTCATTGTGT TGCTGGCCCT GGCAGGCATA
    GCCCACCCTT CGGCCTTCTC CAGCGTCTAC CTGGTGGTGT TCCTGGCCAT CTGCACCTGG
    TGGTCCTGTC ACTTTCCTCT CAGCCCCCTG GGCTTTAACA CCCTCTGTGT CATGGTGAGC
    TGTTTTGGTG CCGGCCATCT CATTTGCCTA TACTGCTATC AGACACCATT TATCCAGGAC
    ATGTTACCCC CTGGGAACAT CTGGGCCAGG CTATTTGGTC TCAAGAACTT CGTAGACCTC
    CCTAACTACT CCAGCCCCAA CGCCCTGGTA CTCAACACTA AGCACGCCTG GCCCATCTAT
    GTGAGTCCCG GAATCCTGCT GCTGCTATAT TACACAGCCA CCTCTCTCCT GAAGCTCCAC
    AAGAGCTGTC CCTCAGAGCT GAGGAAGGAG ACACCCAGGG AGGATGAGGA GCATGAGCTG
    GAACTGGACC ACCTGGAGCC AGAGCCCCAG GCTAGGGACG CCACCCAGGG TGAGATGCCC
    ATGACCACGG AACCTGACCT TGACAACTGC ACCGTGCATG TACTAACCAG CCAGAGCCCT
    GTCCGCCAGC GTCCAGTTCG CCCCAGGCTG GCTGAGCTGA AAGAGATGTC ACCGCTACAT
    GGCCTGGGCC ACCTCATCAT GGACCAGAGC TATGTGTGTG CCCTGATTGC CATGATGGTG
    TGGAGCATCA TGTACCACAG CTGGCTGACC TTCGTCCTGC TGCTCTGGGC CTGCCTCATC
    TGGACGGTGC GTAGCCGCCA CCAGCTGGCT ATGCTCTGCT CGCCCTGCAT CCTGCTGTAT
    GGGCTGACGC TCTGCTGCCT GAGGTACGTG TGGGCCATGG AACTTCCTGA GCTGCCCACC
    ACCCTGGGCC CTGTCAGCCT GCACCAGTTG GGACTGGAAC ACACACGCTA CCCTTGCCTG
    GACCTCGGTG CCATGCTCCT CTATCTGCTC ACATTCTGGC TCCTTCTGCG TCAGTTCGTG
    AAGGAGAAGC TGCTGAAGAA GCAAAAGGTG CCTGCGGCGC TTTTGGAGGT CACGGTGGCC
    GACACTGAGC CCACACAGAC CCAGACGCTG CTGCGGAGCC TGGGGGAGCT GGTCACCGGC
    ATCTACGTCA AATACTGGAT CTATGTGTGC GCCGGCATGT TCATTGTGGT CAGCTTCGCC
    GGCCGCCTGG TGGTCTACAA AATCGTCTAC ATGTTCCTCT TCCTGCTGTG CCTCACCTTG
    TTCCAGGTCT ACTACACCCT GTGGAGGAAG CTGCTGCGTG TCTTCTGGTG GCTCGTGGTG
    GCCTATACAA TGCTCGTGCT CATCGCTGTG TACACCTTCC AGTTCCAGGA CTTCCCCACC
    TATTGGCGCA ACCTCACGGG CTTCACGGAC GAGCAGTTGG GCGACCTGGG CCTGGAGCAG
    TTCAGTGTGT CGGAGCTCTT TTCCAGTATC CTCATCCCTG GCTTCTTCCT GCTGGCCTGC
    ATCCTGCAGC TGCACTACTT CCACAGACCG TTCATGCAGC TCACTGACCT GGAGCACGTG
    CCGCCACCAG GCACCCGCCA CCCTCGATGG GCTCACAGGC AGGATGCAGT GAGCGAGGCC
    CCTCTGCTTG AGCATCAGGA GGAAGAGGAA GTCTTCAGGG AAGATGGGCA GAGCATGGAT
    GGGCCCCACC AGGCCACACA GGTCCCTGAG GGTACGGCCA GCAAGTGGGG CCTGGTGGCT
    GACCGGCTGC TGGACCTAGC GGCCAGCTTC TCGGCTGTCC TCACCCGAAT CCAGGTGTTC
    GTTCGGCGCT TGCTAGAACT TCACGTCTTC AAGCTGGTGG CCCTCTACAC TGTCTGGGTG
    GCCCTGAAAG AAGTGTCTGT GATGAACCTG CTGCTGGTGG TGCTATGGGC CTTCGCCTTG
    CCCTATCCGC GCTTCCGGCC CATGGCTTCC TGCCTGTCCA CCGTGTGGAC CTGTATCATC
    ATTGTATGCA AGATGCTCTA TCAGCTCAAG ATTGTCAACC CGCATGAGTA CTCCAGCAAC
    TGCACTGAGC CCTTCCCCAA CAATACCAAC TTGCAGCCTT TGGAGATCAA CCAGTCTTTG
    CTGTACCGTG GCCCTGTTGA CCCTGCCAAC TGGTTTGGGG TGCGGAAGGG TTACCCCAAC
    TTGGGCTATA TCCAGAACCA CCTGCAGATC CTTCTGTTGC TGGTGTTTGA GGCCGTGGTG
    TACCGGCGCC AAGAGCACTA CCGCCGGCAG CACCAGCAGG CCCCTCTGCC CGCCCAGGCT
    GTGTGCGCAG ATGGCACCCG CCAGAGGTTG GACCAGGACC TACTTAGCTG CCTCAAGTAT
    TTCATCAACT TCTTCTTCTA CAAATTCGGG CTGGAGATCT GCTTCTTGAT GGCCGTGAAT
    GTGATTGGGC AGCGTATGAA CTTCATGGTG ATCTTACACG GTTGCTGGTT GGTGGCCATC
    CTTACACGCC GGCGCCGTGA GGCCATCGCC CGCCTCTGGC CTAACTACTG TCTGTTCCTC
    ACGCTGTTCC TGCTGTACCA GTACCTGCTG TGTTTGGGCA TGCCCCCTGC ACTGTGCATT
    GACTATCCAT GGCGCTGGAG CAAGGCCATC CCCATGAATT CCGCCCTCAT CAAGTGGCTG
    TACCTACCTG ACTTCTTCAG AGCCCCCAAC TCCACCAACC TTATCAGTGA CTTCCTCCTG
    CTGCTTTGCG CCTCCCAGCA GTGGCAGGTC TTCTCAGCGG AGCGAACGGA GGAGTGGCAA
    CGCATGGCGG GCATCAACAC TGACCACCTG GAGCCCCTGC GTGGGGAGCC CAACCCTATA
    CCCAACTTCA TCCACTGCAG GTCCTATCTG GATATGCTGA AGGTGGCCGT CTTCCGCTAC
    CTGTTCTGGC TGGTGCTCGT TGTGGTGTTT GTTGCGGGGG CCACCCGCAT AAGCATCTTC
    GGGCTGGGGT ACCTGCTAGC CTGCTTCTAC CTGCTGCTGT TTGGCACTAC CCTGCTGCAG
    AAGGACACGC GAGCCCAGCT CGTGCTGTGG GACTGCCTCA TCCTCTATAA TGTCACTGTC
    ATCATCTCTA AGAATATGCT GTCGCTCCTG TCCTGTGTCT TCGTGGAGCA AATGCAGAGC
    AACTTCTGCT GGGTCATCCA GCTCTTCAGC CTCGTGTGCA CAGTCAAAGG CTACTATGAT
    CCCAAAGAGA TGATGACCAG GGACCGGGAC TGCCTGCTGC CTGTGGAGGA GGCCGGGATC
    ATCTGGGACA GTATCTGCTT CTTCTTCCTG CTCTTGCAAC GGCGCATCTT TCTCAGCCAC
    TACTTCCTGC ATGTCAGCGC TGACCTGAAA GCCACAGCCC TGCAGGCATC CAGGGGCTTT
    GCCCTCTACA ATGCAGCCAA CCTGAAGAGC ATCAACTTCC ATCGCCAGAT TGAGGAGAAG
    TCCCTGGCCC AGCTGAAAAG ACAGATGAAG CGCATCCGTG CCAAACAGGA GAAGTACAGG
    CAGAGCCAGG CAAGTCGTGG CCAACTCCAG TCCAAAGACC CTCAGGATCC CAGCCAGGAG
    CCAGGGCCTG ACAGCCCAGG GGGCTCCTCC CCGCCACGGA GACAGTGGTG GCGCCCCTGG
    CTGGACCACG CCACAGTCAT CCACTCTGGC GACTACTTCC TGTTTGAGTC AGATAGCGAG
    GAGGAAGAGG AGGCCCTACC TGAGGACCCC AGGCCTGCAG CTCAGAGTGC CTTCCAGATG
    GCATACCAGG CATGGGTAAC CAATGCCCAG ACAGTGCTGA GGCAGCGTCG GGAGCGGGCA
    CGGCAGGAGC GGGCAGAGCA GCTGGCTTCT GGAGGTGACT TGAACCCAGA TGTGGAACCA
    GTAGATGTCC CAGAAGATGA GATGGCAGGC CGTAGCCACA TGATGCAGCG TGTGCTAAGC
    ACCATGCAGT TCCTGTGGGT GCTGGGCCAG GCCACGGTAG ACGGGCTGAC GCGCTGGCTG
    CGTGCATTCA CGAAGCACCA CCGCACCATG AGCGATGTGC TGTGCGCAGA GCGCTACCTG
    CTCACCCAGG AGCTTCTTCG GGTTGGAGAG GTACGCCGAG GTGTGCTGGA CCAGCTTTAT
    GTGGGTGAAG ATGAGGCCAC ATTGTCAGGT CCCATGGAGA CCCGGGATGG ACCCAGCACA
    GCCTCAAGTG GGCTGGGAGC CGAAGAGCCT TTGAGTAGCA TGACAGACGA CACCAGCAGC
    CCCCTGAGCA CAGGCTATAA CACCCGCAGT GGCAGTGAGG AGATTGTCAC CGACGCTGGG
    GACCTCCAGG CTGGGACCTC CCTGCACGGC TCCCAAGAGC TTTTAGCCAA TGCTCGTACC
    CGGATGCGCA CGGCCAGCGA GCTGCTACTG GATAGGCGCC TGCATATCCC TGAGCTGGAG
    GAGGCCGAGC GGTTTGAGGC ACAGCAGGGC CGGACTCTGC GGCTGCTCAG GGCTGGGTAC
    CAGTGCGTGG CGGCACACTC GGAGCTGCTC TGTTACTTCA TCATCATCCT TAACCACATG
    GTGACAGCCT CGGCTGCCTC CCTGGTGCTG CCCGTGCTTG TGTTCCTGTG GGCCATGCTG
    ACCATCCCGA GGCCTAGCAA GCGCTTTTGG ATGACAGCTA TCGTCTTCAC TGAGGTCATG
    GTGGTCACCA AATACCTGTT CCAGTTCGGC TTCTTCCCCT GGAACAGCTA CGTTGTGCTG
    CGGCGCTATG AGAACAAGCC CTACTTCCCT CCGCGAATCC TGGGCCTTGA GAAAACGGAC
    AGCTACATCA AGTATGACCT GGTGCAGCTC ATGGCCCTCT TCTTCCACCG CTCGCAGCTA
    CTGTGTTATG GCCTCTGGGA CCATGAGGAG GATCGCTATC CCAAGGACCA TTGCAGGAGT
    AGTGTGAAGG ACCGGGAGGC CAAGGAAGAG CCAGAAGCTA AGCTGGAATC GCAGTCTGAG
    ACAGGCACTG GGCATCCCAA GGAGCCAGTG TTGGCCGGTA CTCCCAGGGA CCACATCCAA
    GGGAAAGGAA GTATTAGATC CAAGGATGTT ATCCAAGATC CCCCAGAGGA CCTTAAGCCC
    CGGCACACGA GGCACATCAG CATACGCTTC AGGAGGCGCA AGGAGACTCC AGGACCCAAA
    GGAACAGCAG TCATGGAGAC TGAGCACGAG GAGGGAGAAG GAAAAGAAAC TACAGAGAGA
    AAGAGGCCGC GTCACACTCA AGAAAAATCG AAGTTTCGGG AGAGAATGAA GGCAGCTGGG
    CGCCGGCTGC AGAGCTTCTG TGTGTCACTG GCCCAGAGCT TCTACCAACC CTTGCAGCGT
    TTCTTCCATG ACATTCTGCA CACAAAGTAC CGGGCGGCCA CCGACGTCTA CGCCCTCATG
    TTCCTGGCCG ATATTGTCGA CATCATCATC ATCATCTTTG GTTTTTGGGC TTTTGGGAAG
    CACTCTGCAG CCACAGACAT TGCATCCTCG CTGTCAGATG ACCAGGTGCC ACAGGCCTTC
    CTGTTCATGC TGCTGGTCCA GTTTGGCACC ATGGTCATCG ACCGTGCCCT CTACCTGCGC
    AAGACTGTCC TGGGAAAGCT GGCCTTTCAG GTGGTCCTGG TGGTGGCGAT TCACATCTGG
    ATGTTCTTTA TCTTACCGGC TGTCACTGAG AGGATGTTCA GCCAGAATGC GGTGGCACAG
    CTGTGGTACT TCGTCAAGTG CATTTACTTT GCCCTGTCCG CCTACCAGAT CCGCTGTGGC
    TACCCCACCC GTATCTTGGG CAACTTCCTC ACCAAGAAAT ACAACCATCT AAACCTCTTC
    CTTTTCCAGG GGTTCCGTCT AGTGCCGTTC CTGGTGGAGC TGCGGGCCGT CATGGACTGG
    GTGTGGACCG ACACCACGCT GTCCCTGTCC AACTGGATGT GTGTGGAAGA CATCTATGCC
    AACATCTTTA TCATCAAGTG CAGCCGAGAG ACAGAGAAGA AATACCCCCA GCCCAAGGGG
    CAGAAGAAGA AGAAAATTGT CAAGTATGGT ATGGGAGGCC TCATTATCCT CTTCCTCATC
    GCCATCATCT GGTTCCCTCT GCTCTTCATG TCACTGATCC GCTCTGTGGT CGGGGTCGTC
    AACCAGCCCA TTGATGTCAC CGTCACCCTC AAGCTAGGCG GCTATGAGCC ACTGTTCACC
    ATGAGCGCCC AGCAGCCATC CATTGTGCCA TTCACACCCC AGGCCTACGA GGAGCTGTCC
    CAGCAGTTTG ACCCCTATCC ACTAGCCATG CAGTTCATTA GCCAGTACAG TCCTGAGGAC
    ATCGTCACTG CACAGATCGA GGGCAGCTCG GGGGCGCTGT GGCGCATCAG CCCACCCAGC
    CGAGCCCAGA TGAAGCAGGA GCTGTACAAC GGCACAGCCG ACATTACACT GCGCTTTACC
    TGGAATTTCC AAAGGGACCT GGCCAAGGGT GGCACTGTGG AGTATACTAA TGAGAAGCAC
    ACCTTGGAGC TGGCCCCCAA CAGTACGGCA CGAAGGCAGC TGGCCCAACT GCTCGAGGGC
    AGACCTGACC AGTCAGTGGT CATTCCCCAT CTCTTCCCCA AGTACATTCG TGCTCCCAAT
    GGGCCTGAAG CCAACCCTGT GAAGCAGCTG CAGCCAGATG AGGAAGAGGA CTACCTTGGT
    GTGCGCATCC AGCTGCGGAG GGAGCAAGTG GGCACAGGGG CCTCTGGGGA GCAAGCGGGC
    ACCAAGGCCT CCGACTTCCT CGAGTGGTGG GTCATCGAGC TGCAGGACTG CAAGGCTGAC
    TGCAACCTGC TGCCCATGGT CATCTTCAGT GACAAGGTCA GCCCACCTAG CCTGGGCTTC
    CTGGCCGGCT ACGGGATTGT GGGGCTGTAC GTCTCCATCG TGCTGGTGGT TGGCAAGTTT
    GTGCGGGGCT TCTTCAGCGA GATCTCTCAC TCCATCATGT TCGAGGAACT GCCGTGTGTG
    GACCGCATCC TCAAGCTGTG CCAGGACATC TTCTTGGTGC GCGAGACCCG GGAGCTGGAG
    CTGGAGGAGG AGCTATACGC CAAGCTCATC TTCCTGTACC GATCTCCAGA GACCATGATT
    AAGTGGACAC GTGAGAGGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu41422
    Clone ID Related Accession (Same CDS sequence) NM_001357349.1 , XM_006530955.2
    Accession Version XM_006530955.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product piezo-type mechanosensitive ion channel component 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTGCCCTG CACGCTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGCTGGTCT ATTTGCTGTT TCTACTGCTG
    CTGCCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTC
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ACCTTGCCTT TCAGATATGC
    CTACACACCG TGCCTCACCT GGACCAGTTT CTGGGACAAA ACGGTAGCCT TTGGGTGAAG
    GTGTCTCAAC ACATAGGGGT TACGAGGCTG GACCTGAAGG ACATCTTTAA CACCACCAGG
    CTGGTAGCAC CTGACCTGGG AGTGCTGCTG GCGTCCTCCC TTTGCCTTGG CCTCTGTGGA
    CGCCTCACGA GGAAAGCCCG GCAGAGTCGG CGCACCCAGG AGCTGCAGGA TGATGACGAT
    GACGATGATG ACGACGATGA AGACATAGAT GCTGCCCCAG CCGTGGGGCT GAAGGGAGCC
    CCTGCCCTGG CAACCAAACG CAGGCTGTGG CTGGCCTCCC GCTTCCGGGT CACGGCCCAC
    TGGCTGCTGA TGACCTCTGG ACGGACGCTG GTCATTGTGT TGCTGGCCCT GGCAGGCATA
    GCCCACCCTT CGGCCTTCTC CAGCGTCTAC CTGGTGGTGT TCCTGGCCAT CTGCACCTGG
    TGGTCCTGTC ACTTTCCTCT CAGCCCCCTG GGCTTTAACA CCCTCTGTGT CATGGTGAGC
    TGTTTTGGTG CCGGCCATCT CATTTGCCTA TACTGCTATC AGACACCATT TATCCAGGAC
    ATGTTACCCC CTGGGAACAT CTGGGCCAGG CTATTTGGTC TCAAGAACTT CGTAGACCTC
    CCTAACTACT CCAGCCCCAA CGCCCTGGTA CTCAACACTA AGCACGCCTG GCCCATCTAT
    GTGAGTCCCG GAATCCTGCT GCTGCTATAT TACACAGCCA CCTCTCTCCT GAAGCTCCAC
    AAGAGCTGTC CCTCAGAGCT GAGGAAGGAG ACACCCAGGG AGGATGAGGA GCATGAGCTG
    GAACTGGACC ACCTGGAGCC AGAGCCCCAG GCTAGGGACG CCACCCAGGG TGAGATGCCC
    ATGACCACGG AACCTGACCT TGACAACTGC ACCGTGCATG TACTAACCAG CCAGAGCCCT
    GTCCGCCAGC GTCCAGTTCG CCCCAGGCTG GCTGAGCTGA AAGAGATGTC ACCGCTACAT
    GGCCTGGGCC ACCTCATCAT GGACCAGAGC TATGTGTGTG CCCTGATTGC CATGATGGTG
    TGGAGCATCA TGTACCACAG CTGGCTGACC TTCGTCCTGC TGCTCTGGGC CTGCCTCATC
    TGGACGGTGC GTAGCCGCCA CCAGCTGGCT ATGCTCTGCT CGCCCTGCAT CCTGCTGTAT
    GGGCTGACGC TCTGCTGCCT GAGGTACGTG TGGGCCATGG AACTTCCTGA GCTGCCCACC
    ACCCTGGGCC CTGTCAGCCT GCACCAGTTG GGACTGGAAC ACACACGCTA CCCTTGCCTG
    GACCTCGGTG CCATGCTCCT CTATCTGCTC ACATTCTGGC TCCTTCTGCG TCAGTTCGTG
    AAGGAGAAGC TGCTGAAGAA GCAAAAGGTG CCTGCGGCGC TTTTGGAGGT CACGGTGGCC
    GACACTGAGC CCACACAGAC CCAGACGCTG CTGCGGAGCC TGGGGGAGCT GGTCACCGGC
    ATCTACGTCA AATACTGGAT CTATGTGTGC GCCGGCATGT TCATTGTGGT CAGCTTCGCC
    GGCCGCCTGG TGGTCTACAA AATCGTCTAC ATGTTCCTCT TCCTGCTGTG CCTCACCTTG
    TTCCAGGTCT ACTACACCCT GTGGAGGAAG CTGCTGCGTG TCTTCTGGTG GCTCGTGGTG
    GCCTATACAA TGCTCGTGCT CATCGCTGTG TACACCTTCC AGTTCCAGGA CTTCCCCACC
    TATTGGCGCA ACCTCACGGG CTTCACGGAC GAGCAGTTGG GCGACCTGGG CCTGGAGCAG
    TTCAGTGTGT CGGAGCTCTT TTCCAGTATC CTCATCCCTG GCTTCTTCCT GCTGGCCTGC
    ATCCTGCAGC TGCACTACTT CCACAGACCG TTCATGCAGC TCACTGACCT GGAGCACGTG
    CCGCCACCAG GCACCCGCCA CCCTCGATGG GCTCACAGGC AGGATGCAGT GAGCGAGGCC
    CCTCTGCTTG AGCATCAGGA GGAAGAGGAA GTCTTCAGGG AAGATGGGCA GAGCATGGAT
    GGGCCCCACC AGGCCACACA GGTCCCTGAG GGTACGGCCA GCAAGTGGGG CCTGGTGGCT
    GACCGGCTGC TGGACCTAGC GGCCAGCTTC TCGGCTGTCC TCACCCGAAT CCAGGTGTTC
    GTTCGGCGCT TGCTAGAACT TCACGTCTTC AAGCTGGTGG CCCTCTACAC TGTCTGGGTG
    GCCCTGAAAG AAGTGTCTGT GATGAACCTG CTGCTGGTGG TGCTATGGGC CTTCGCCTTG
    CCCTATCCGC GCTTCCGGCC CATGGCTTCC TGCCTGTCCA CCGTGTGGAC CTGTATCATC
    ATTGTATGCA AGATGCTCTA TCAGCTCAAG ATTGTCAACC CGCATGAGTA CTCCAGCAAC
    TGCACTGAGC CCTTCCCCAA CAATACCAAC TTGCAGCCTT TGGAGATCAA CCAGTCTTTG
    CTGTACCGTG GCCCTGTTGA CCCTGCCAAC TGGTTTGGGG TGCGGAAGGG TTACCCCAAC
    TTGGGCTATA TCCAGAACCA CCTGCAGATC CTTCTGTTGC TGGTGTTTGA GGCCGTGGTG
    TACCGGCGCC AAGAGCACTA CCGCCGGCAG CACCAGCAGG CCCCTCTGCC CGCCCAGGCT
    GTGTGCGCAG ATGGCACCCG CCAGAGGTTG GACCAGGACC TACTTAGCTG CCTCAAGTAT
    TTCATCAACT TCTTCTTCTA CAAATTCGGG CTGGAGATCT GCTTCTTGAT GGCCGTGAAT
    GTGATTGGGC AGCGTATGAA CTTCATGGTG ATCTTACACG GTTGCTGGTT GGTGGCCATC
    CTTACACGCC GGCGCCGTGA GGCCATCGCC CGCCTCTGGC CTAACTACTG TCTGTTCCTC
    ACGCTGTTCC TGCTGTACCA GTACCTGCTG TGTTTGGGCA TGCCCCCTGC ACTGTGCATT
    GACTATCCAT GGCGCTGGAG CAAGGCCATC CCCATGAATT CCGCCCTCAT CAAGTGGCTG
    TACCTACCTG ACTTCTTCAG AGCCCCCAAC TCCACCAACC TTATCAGTGA CTTCCTCCTG
    CTGCTTTGCG CCTCCCAGCA GTGGCAGGTC TTCTCAGCGG AGCGAACGGA GGAGTGGCAA
    CGCATGGCGG GCATCAACAC TGACCACCTG GAGCCCCTGC GTGGGGAGCC CAACCCTATA
    CCCAACTTCA TCCACTGCAG GTCCTATCTG GATATGCTGA AGGTGGCCGT CTTCCGCTAC
    CTGTTCTGGC TGGTGCTCGT TGTGGTGTTT GTTGCGGGGG CCACCCGCAT AAGCATCTTC
    GGGCTGGGGT ACCTGCTAGC CTGCTTCTAC CTGCTGCTGT TTGGCACTAC CCTGCTGCAG
    AAGGACACGC GAGCCCAGCT CGTGCTGTGG GACTGCCTCA TCCTCTATAA TGTCACTGTC
    ATCATCTCTA AGAATATGCT GTCGCTCCTG TCCTGTGTCT TCGTGGAGCA AATGCAGAGC
    AACTTCTGCT GGGTCATCCA GCTCTTCAGC CTCGTGTGCA CAGTCAAAGG CTACTATGAT
    CCCAAAGAGA TGATGACCAG GGACCGGGAC TGCCTGCTGC CTGTGGAGGA GGCCGGGATC
    ATCTGGGACA GTATCTGCTT CTTCTTCCTG CTCTTGCAAC GGCGCATCTT TCTCAGCCAC
    TACTTCCTGC ATGTCAGCGC TGACCTGAAA GCCACAGCCC TGCAGGCATC CAGGGGCTTT
    GCCCTCTACA ATGCAGCCAA CCTGAAGAGC ATCAACTTCC ATCGCCAGAT TGAGGAGAAG
    TCCCTGGCCC AGCTGAAAAG ACAGATGAAG CGCATCCGTG CCAAACAGGA GAAGTACAGG
    CAGAGCCAGG CAAGTCGTGG CCAACTCCAG TCCAAAGACC CTCAGGATCC CAGCCAGGAG
    CCAGGGCCTG ACAGCCCAGG GGGCTCCTCC CCGCCACGGA GACAGTGGTG GCGCCCCTGG
    CTGGACCACG CCACAGTCAT CCACTCTGGC GACTACTTCC TGTTTGAGTC AGATAGCGAG
    GAGGAAGAGG AGGCCCTACC TGAGGACCCC AGGCCTGCAG CTCAGAGTGC CTTCCAGATG
    GCATACCAGG CATGGGTAAC CAATGCCCAG ACAGTGCTGA GGCAGCGTCG GGAGCGGGCA
    CGGCAGGAGC GGGCAGAGCA GCTGGCTTCT GGAGGTGACT TGAACCCAGA TGTGGAACCA
    GTAGATGTCC CAGAAGATGA GATGGCAGGC CGTAGCCACA TGATGCAGCG TGTGCTAAGC
    ACCATGCAGT TCCTGTGGGT GCTGGGCCAG GCCACGGTAG ACGGGCTGAC GCGCTGGCTG
    CGTGCATTCA CGAAGCACCA CCGCACCATG AGCGATGTGC TGTGCGCAGA GCGCTACCTG
    CTCACCCAGG AGCTTCTTCG GGTTGGAGAG GTACGCCGAG GTGTGCTGGA CCAGCTTTAT
    GTGGGTGAAG ATGAGGCCAC ATTGTCAGGT CCCATGGAGA CCCGGGATGG ACCCAGCACA
    GCCTCAAGTG GGCTGGGAGC CGAAGAGCCT TTGAGTAGCA TGACAGACGA CACCAGCAGC
    CCCCTGAGCA CAGGCTATAA CACCCGCAGT GGCAGTGAGG AGATTGTCAC CGACGCTGGG
    GACCTCCAGG CTGGGACCTC CCTGCACGGC TCCCAAGAGC TTTTAGCCAA TGCTCGTACC
    CGGATGCGCA CGGCCAGCGA GCTGCTACTG GATAGGCGCC TGCATATCCC TGAGCTGGAG
    GAGGCCGAGC GGTTTGAGGC ACAGCAGGGC CGGACTCTGC GGCTGCTCAG GGCTGGGTAC
    CAGTGCGTGG CGGCACACTC GGAGCTGCTC TGTTACTTCA TCATCATCCT TAACCACATG
    GTGACAGCCT CGGCTGCCTC CCTGGTGCTG CCCGTGCTTG TGTTCCTGTG GGCCATGCTG
    ACCATCCCGA GGCCTAGCAA GCGCTTTTGG ATGACAGCTA TCGTCTTCAC TGAGGTCATG
    GTGGTCACCA AATACCTGTT CCAGTTCGGC TTCTTCCCCT GGAACAGCTA CGTTGTGCTG
    CGGCGCTATG AGAACAAGCC CTACTTCCCT CCGCGAATCC TGGGCCTTGA GAAAACGGAC
    AGCTACATCA AGTATGACCT GGTGCAGCTC ATGGCCCTCT TCTTCCACCG CTCGCAGCTA
    CTGTGTTATG GCCTCTGGGA CCATGAGGAG GATCGCTATC CCAAGGACCA TTGCAGGAGT
    AGTGTGAAGG ACCGGGAGGC CAAGGAAGAG CCAGAAGCTA AGCTGGAATC GCAGTCTGAG
    ACAGGCACTG GGCATCCCAA GGAGCCAGTG TTGGCCGGTA CTCCCAGGGA CCACATCCAA
    GGGAAAGGAA GTATTAGATC CAAGGATGTT ATCCAAGATC CCCCAGAGGA CCTTAAGCCC
    CGGCACACGA GGCACATCAG CATACGCTTC AGGAGGCGCA AGGAGACTCC AGGACCCAAA
    GGAACAGCAG TCATGGAGAC TGAGCACGAG GAGGGAGAAG GAAAAGAAAC TACAGAGAGA
    AAGAGGCCGC GTCACACTCA AGAAAAATCG AAGTTTCGGG AGAGAATGAA GGCAGCTGGG
    CGCCGGCTGC AGAGCTTCTG TGTGTCACTG GCCCAGAGCT TCTACCAACC CTTGCAGCGT
    TTCTTCCATG ACATTCTGCA CACAAAGTAC CGGGCGGCCA CCGACGTCTA CGCCCTCATG
    TTCCTGGCCG ATATTGTCGA CATCATCATC ATCATCTTTG GTTTTTGGGC TTTTGGGAAG
    CACTCTGCAG CCACAGACAT TGCATCCTCG CTGTCAGATG ACCAGGTGCC ACAGGCCTTC
    CTGTTCATGC TGCTGGTCCA GTTTGGCACC ATGGTCATCG ACCGTGCCCT CTACCTGCGC
    AAGACTGTCC TGGGAAAGCT GGCCTTTCAG GTGGTCCTGG TGGTGGCGAT TCACATCTGG
    ATGTTCTTTA TCTTACCGGC TGTCACTGAG AGGATGTTCA GCCAGAATGC GGTGGCACAG
    CTGTGGTACT TCGTCAAGTG CATTTACTTT GCCCTGTCCG CCTACCAGAT CCGCTGTGGC
    TACCCCACCC GTATCTTGGG CAACTTCCTC ACCAAGAAAT ACAACCATCT AAACCTCTTC
    CTTTTCCAGG GGTTCCGTCT AGTGCCGTTC CTGGTGGAGC TGCGGGCCGT CATGGACTGG
    GTGTGGACCG ACACCACGCT GTCCCTGTCC AACTGGATGT GTGTGGAAGA CATCTATGCC
    AACATCTTTA TCATCAAGTG CAGCCGAGAG ACAGAGAAGA AATACCCCCA GCCCAAGGGG
    CAGAAGAAGA AGAAAATTGT CAAGTATGGT ATGGGAGGCC TCATTATCCT CTTCCTCATC
    GCCATCATCT GGTTCCCTCT GCTCTTCATG TCACTGATCC GCTCTGTGGT CGGGGTCGTC
    AACCAGCCCA TTGATGTCAC CGTCACCCTC AAGCTAGGCG GCTATGAGCC ACTGTTCACC
    ATGAGCGCCC AGCAGCCATC CATTGTGCCA TTCACACCCC AGGCCTACGA GGAGCTGTCC
    CAGCAGTTTG ACCCCTATCC ACTAGCCATG CAGTTCATTA GCCAGTACAG TCCTGAGGAC
    ATCGTCACTG CACAGATCGA GGGCAGCTCG GGGGCGCTGT GGCGCATCAG CCCACCCAGC
    CGAGCCCAGA TGAAGCAGGA GCTGTACAAC GGCACAGCCG ACATTACACT GCGCTTTACC
    TGGAATTTCC AAAGGGACCT GGCCAAGGGT GGCACTGTGG AGTATACTAA TGAGAAGCAC
    ACCTTGGAGC TGGCCCCCAA CAGTACGGCA CGAAGGCAGC TGGCCCAACT GCTCGAGGGC
    AGACCTGACC AGTCAGTGGT CATTCCCCAT CTCTTCCCCA AGTACATTCG TGCTCCCAAT
    GGGCCTGAAG CCAACCCTGT GAAGCAGCTG CAGCCAGATG AGGAAGAGGA CTACCTTGGT
    GTGCGCATCC AGCTGCGGAG GGAGCAAGTG GGCACAGGGG CCTCTGGGGA GCAAGCGGGC
    ACCAAGGCCT CCGACTTCCT CGAGTGGTGG GTCATCGAGC TGCAGGACTG CAAGGCTGAC
    TGCAACCTGC TGCCCATGGT CATCTTCAGT GACAAGGTCA GCCCACCTAG CCTGGGCTTC
    CTGGCCGGCT ACGGGATTGT GGGGCTGTAC GTCTCCATCG TGCTGGTGGT TGGCAAGTTT
    GTGCGGGGCT TCTTCAGCGA GATCTCTCAC TCCATCATGT TCGAGGAACT GCCGTGTGTG
    GACCGCATCC TCAAGCTGTG CCAGGACATC TTCTTGGTGC GCGAGACCCG GGAGCTGGAG
    CTGGAGGAGG AGCTATACGC CAAGCTCATC TTCCTGTACC GATCTCCAGA GACCATGATT
    AAGTGGACAC GTGAGAGGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006531018.1
    CDS620..8263
    Translation

    Target ORF information:

    RefSeq Version XM_006530955.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530955.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTGCCCTG CACGCTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGCTGGTCT ATTTGCTGTT TCTACTGCTG
    CTGCCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTC
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ACCTTGCCTT TCAGATATGC
    CTACACACCG TGCCTCACCT GGACCAGTTT CTGGGACAAA ACGGTAGCCT TTGGGTGAAG
    GTGTCTCAAC ACATAGGGGT TACGAGGCTG GACCTGAAGG ACATCTTTAA CACCACCAGG
    CTGGTAGCAC CTGACCTGGG AGTGCTGCTG GCGTCCTCCC TTTGCCTTGG CCTCTGTGGA
    CGCCTCACGA GGAAAGCCCG GCAGAGTCGG CGCACCCAGG AGCTGCAGGA TGATGACGAT
    GACGATGATG ACGACGATGA AGACATAGAT GCTGCCCCAG CCGTGGGGCT GAAGGGAGCC
    CCTGCCCTGG CAACCAAACG CAGGCTGTGG CTGGCCTCCC GCTTCCGGGT CACGGCCCAC
    TGGCTGCTGA TGACCTCTGG ACGGACGCTG GTCATTGTGT TGCTGGCCCT GGCAGGCATA
    GCCCACCCTT CGGCCTTCTC CAGCGTCTAC CTGGTGGTGT TCCTGGCCAT CTGCACCTGG
    TGGTCCTGTC ACTTTCCTCT CAGCCCCCTG GGCTTTAACA CCCTCTGTGT CATGGTGAGC
    TGTTTTGGTG CCGGCCATCT CATTTGCCTA TACTGCTATC AGACACCATT TATCCAGGAC
    ATGTTACCCC CTGGGAACAT CTGGGCCAGG CTATTTGGTC TCAAGAACTT CGTAGACCTC
    CCTAACTACT CCAGCCCCAA CGCCCTGGTA CTCAACACTA AGCACGCCTG GCCCATCTAT
    GTGAGTCCCG GAATCCTGCT GCTGCTATAT TACACAGCCA CCTCTCTCCT GAAGCTCCAC
    AAGAGCTGTC CCTCAGAGCT GAGGAAGGAG ACACCCAGGG AGGATGAGGA GCATGAGCTG
    GAACTGGACC ACCTGGAGCC AGAGCCCCAG GCTAGGGACG CCACCCAGGG TGAGATGCCC
    ATGACCACGG AACCTGACCT TGACAACTGC ACCGTGCATG TACTAACCAG CCAGAGCCCT
    GTCCGCCAGC GTCCAGTTCG CCCCAGGCTG GCTGAGCTGA AAGAGATGTC ACCGCTACAT
    GGCCTGGGCC ACCTCATCAT GGACCAGAGC TATGTGTGTG CCCTGATTGC CATGATGGTG
    TGGAGCATCA TGTACCACAG CTGGCTGACC TTCGTCCTGC TGCTCTGGGC CTGCCTCATC
    TGGACGGTGC GTAGCCGCCA CCAGCTGGCT ATGCTCTGCT CGCCCTGCAT CCTGCTGTAT
    GGGCTGACGC TCTGCTGCCT GAGGTACGTG TGGGCCATGG AACTTCCTGA GCTGCCCACC
    ACCCTGGGCC CTGTCAGCCT GCACCAGTTG GGACTGGAAC ACACACGCTA CCCTTGCCTG
    GACCTCGGTG CCATGCTCCT CTATCTGCTC ACATTCTGGC TCCTTCTGCG TCAGTTCGTG
    AAGGAGAAGC TGCTGAAGAA GCAAAAGGTG CCTGCGGCGC TTTTGGAGGT CACGGTGGCC
    GACACTGAGC CCACACAGAC CCAGACGCTG CTGCGGAGCC TGGGGGAGCT GGTCACCGGC
    ATCTACGTCA AATACTGGAT CTATGTGTGC GCCGGCATGT TCATTGTGGT CAGCTTCGCC
    GGCCGCCTGG TGGTCTACAA AATCGTCTAC ATGTTCCTCT TCCTGCTGTG CCTCACCTTG
    TTCCAGGTCT ACTACACCCT GTGGAGGAAG CTGCTGCGTG TCTTCTGGTG GCTCGTGGTG
    GCCTATACAA TGCTCGTGCT CATCGCTGTG TACACCTTCC AGTTCCAGGA CTTCCCCACC
    TATTGGCGCA ACCTCACGGG CTTCACGGAC GAGCAGTTGG GCGACCTGGG CCTGGAGCAG
    TTCAGTGTGT CGGAGCTCTT TTCCAGTATC CTCATCCCTG GCTTCTTCCT GCTGGCCTGC
    ATCCTGCAGC TGCACTACTT CCACAGACCG TTCATGCAGC TCACTGACCT GGAGCACGTG
    CCGCCACCAG GCACCCGCCA CCCTCGATGG GCTCACAGGC AGGATGCAGT GAGCGAGGCC
    CCTCTGCTTG AGCATCAGGA GGAAGAGGAA GTCTTCAGGG AAGATGGGCA GAGCATGGAT
    GGGCCCCACC AGGCCACACA GGTCCCTGAG GGTACGGCCA GCAAGTGGGG CCTGGTGGCT
    GACCGGCTGC TGGACCTAGC GGCCAGCTTC TCGGCTGTCC TCACCCGAAT CCAGGTGTTC
    GTTCGGCGCT TGCTAGAACT TCACGTCTTC AAGCTGGTGG CCCTCTACAC TGTCTGGGTG
    GCCCTGAAAG AAGTGTCTGT GATGAACCTG CTGCTGGTGG TGCTATGGGC CTTCGCCTTG
    CCCTATCCGC GCTTCCGGCC CATGGCTTCC TGCCTGTCCA CCGTGTGGAC CTGTATCATC
    ATTGTATGCA AGATGCTCTA TCAGCTCAAG ATTGTCAACC CGCATGAGTA CTCCAGCAAC
    TGCACTGAGC CCTTCCCCAA CAATACCAAC TTGCAGCCTT TGGAGATCAA CCAGTCTTTG
    CTGTACCGTG GCCCTGTTGA CCCTGCCAAC TGGTTTGGGG TGCGGAAGGG TTACCCCAAC
    TTGGGCTATA TCCAGAACCA CCTGCAGATC CTTCTGTTGC TGGTGTTTGA GGCCGTGGTG
    TACCGGCGCC AAGAGCACTA CCGCCGGCAG CACCAGCAGG CCCCTCTGCC CGCCCAGGCT
    GTGTGCGCAG ATGGCACCCG CCAGAGGTTG GACCAGGACC TACTTAGCTG CCTCAAGTAT
    TTCATCAACT TCTTCTTCTA CAAATTCGGG CTGGAGATCT GCTTCTTGAT GGCCGTGAAT
    GTGATTGGGC AGCGTATGAA CTTCATGGTG ATCTTACACG GTTGCTGGTT GGTGGCCATC
    CTTACACGCC GGCGCCGTGA GGCCATCGCC CGCCTCTGGC CTAACTACTG TCTGTTCCTC
    ACGCTGTTCC TGCTGTACCA GTACCTGCTG TGTTTGGGCA TGCCCCCTGC ACTGTGCATT
    GACTATCCAT GGCGCTGGAG CAAGGCCATC CCCATGAATT CCGCCCTCAT CAAGTGGCTG
    TACCTACCTG ACTTCTTCAG AGCCCCCAAC TCCACCAACC TTATCAGTGA CTTCCTCCTG
    CTGCTTTGCG CCTCCCAGCA GTGGCAGGTC TTCTCAGCGG AGCGAACGGA GGAGTGGCAA
    CGCATGGCGG GCATCAACAC TGACCACCTG GAGCCCCTGC GTGGGGAGCC CAACCCTATA
    CCCAACTTCA TCCACTGCAG GTCCTATCTG GATATGCTGA AGGTGGCCGT CTTCCGCTAC
    CTGTTCTGGC TGGTGCTCGT TGTGGTGTTT GTTGCGGGGG CCACCCGCAT AAGCATCTTC
    GGGCTGGGGT ACCTGCTAGC CTGCTTCTAC CTGCTGCTGT TTGGCACTAC CCTGCTGCAG
    AAGGACACGC GAGCCCAGCT CGTGCTGTGG GACTGCCTCA TCCTCTATAA TGTCACTGTC
    ATCATCTCTA AGAATATGCT GTCGCTCCTG TCCTGTGTCT TCGTGGAGCA AATGCAGAGC
    AACTTCTGCT GGGTCATCCA GCTCTTCAGC CTCGTGTGCA CAGTCAAAGG CTACTATGAT
    CCCAAAGAGA TGATGACCAG GGACCGGGAC TGCCTGCTGC CTGTGGAGGA GGCCGGGATC
    ATCTGGGACA GTATCTGCTT CTTCTTCCTG CTCTTGCAAC GGCGCATCTT TCTCAGCCAC
    TACTTCCTGC ATGTCAGCGC TGACCTGAAA GCCACAGCCC TGCAGGCATC CAGGGGCTTT
    GCCCTCTACA ATGCAGCCAA CCTGAAGAGC ATCAACTTCC ATCGCCAGAT TGAGGAGAAG
    TCCCTGGCCC AGCTGAAAAG ACAGATGAAG CGCATCCGTG CCAAACAGGA GAAGTACAGG
    CAGAGCCAGG CAAGTCGTGG CCAACTCCAG TCCAAAGACC CTCAGGATCC CAGCCAGGAG
    CCAGGGCCTG ACAGCCCAGG GGGCTCCTCC CCGCCACGGA GACAGTGGTG GCGCCCCTGG
    CTGGACCACG CCACAGTCAT CCACTCTGGC GACTACTTCC TGTTTGAGTC AGATAGCGAG
    GAGGAAGAGG AGGCCCTACC TGAGGACCCC AGGCCTGCAG CTCAGAGTGC CTTCCAGATG
    GCATACCAGG CATGGGTAAC CAATGCCCAG ACAGTGCTGA GGCAGCGTCG GGAGCGGGCA
    CGGCAGGAGC GGGCAGAGCA GCTGGCTTCT GGAGGTGACT TGAACCCAGA TGTGGAACCA
    GTAGATGTCC CAGAAGATGA GATGGCAGGC CGTAGCCACA TGATGCAGCG TGTGCTAAGC
    ACCATGCAGT TCCTGTGGGT GCTGGGCCAG GCCACGGTAG ACGGGCTGAC GCGCTGGCTG
    CGTGCATTCA CGAAGCACCA CCGCACCATG AGCGATGTGC TGTGCGCAGA GCGCTACCTG
    CTCACCCAGG AGCTTCTTCG GGTTGGAGAG GTACGCCGAG GTGTGCTGGA CCAGCTTTAT
    GTGGGTGAAG ATGAGGCCAC ATTGTCAGGT CCCATGGAGA CCCGGGATGG ACCCAGCACA
    GCCTCAAGTG GGCTGGGAGC CGAAGAGCCT TTGAGTAGCA TGACAGACGA CACCAGCAGC
    CCCCTGAGCA CAGGCTATAA CACCCGCAGT GGCAGTGAGG AGATTGTCAC CGACGCTGGG
    GACCTCCAGG CTGGGACCTC CCTGCACGGC TCCCAAGAGC TTTTAGCCAA TGCTCGTACC
    CGGATGCGCA CGGCCAGCGA GCTGCTACTG GATAGGCGCC TGCATATCCC TGAGCTGGAG
    GAGGCCGAGC GGTTTGAGGC ACAGCAGGGC CGGACTCTGC GGCTGCTCAG GGCTGGGTAC
    CAGTGCGTGG CGGCACACTC GGAGCTGCTC TGTTACTTCA TCATCATCCT TAACCACATG
    GTGACAGCCT CGGCTGCCTC CCTGGTGCTG CCCGTGCTTG TGTTCCTGTG GGCCATGCTG
    ACCATCCCGA GGCCTAGCAA GCGCTTTTGG ATGACAGCTA TCGTCTTCAC TGAGGTCATG
    GTGGTCACCA AATACCTGTT CCAGTTCGGC TTCTTCCCCT GGAACAGCTA CGTTGTGCTG
    CGGCGCTATG AGAACAAGCC CTACTTCCCT CCGCGAATCC TGGGCCTTGA GAAAACGGAC
    AGCTACATCA AGTATGACCT GGTGCAGCTC ATGGCCCTCT TCTTCCACCG CTCGCAGCTA
    CTGTGTTATG GCCTCTGGGA CCATGAGGAG GATCGCTATC CCAAGGACCA TTGCAGGAGT
    AGTGTGAAGG ACCGGGAGGC CAAGGAAGAG CCAGAAGCTA AGCTGGAATC GCAGTCTGAG
    ACAGGCACTG GGCATCCCAA GGAGCCAGTG TTGGCCGGTA CTCCCAGGGA CCACATCCAA
    GGGAAAGGAA GTATTAGATC CAAGGATGTT ATCCAAGATC CCCCAGAGGA CCTTAAGCCC
    CGGCACACGA GGCACATCAG CATACGCTTC AGGAGGCGCA AGGAGACTCC AGGACCCAAA
    GGAACAGCAG TCATGGAGAC TGAGCACGAG GAGGGAGAAG GAAAAGAAAC TACAGAGAGA
    AAGAGGCCGC GTCACACTCA AGAAAAATCG AAGTTTCGGG AGAGAATGAA GGCAGCTGGG
    CGCCGGCTGC AGAGCTTCTG TGTGTCACTG GCCCAGAGCT TCTACCAACC CTTGCAGCGT
    TTCTTCCATG ACATTCTGCA CACAAAGTAC CGGGCGGCCA CCGACGTCTA CGCCCTCATG
    TTCCTGGCCG ATATTGTCGA CATCATCATC ATCATCTTTG GTTTTTGGGC TTTTGGGAAG
    CACTCTGCAG CCACAGACAT TGCATCCTCG CTGTCAGATG ACCAGGTGCC ACAGGCCTTC
    CTGTTCATGC TGCTGGTCCA GTTTGGCACC ATGGTCATCG ACCGTGCCCT CTACCTGCGC
    AAGACTGTCC TGGGAAAGCT GGCCTTTCAG GTGGTCCTGG TGGTGGCGAT TCACATCTGG
    ATGTTCTTTA TCTTACCGGC TGTCACTGAG AGGATGTTCA GCCAGAATGC GGTGGCACAG
    CTGTGGTACT TCGTCAAGTG CATTTACTTT GCCCTGTCCG CCTACCAGAT CCGCTGTGGC
    TACCCCACCC GTATCTTGGG CAACTTCCTC ACCAAGAAAT ACAACCATCT AAACCTCTTC
    CTTTTCCAGG GGTTCCGTCT AGTGCCGTTC CTGGTGGAGC TGCGGGCCGT CATGGACTGG
    GTGTGGACCG ACACCACGCT GTCCCTGTCC AACTGGATGT GTGTGGAAGA CATCTATGCC
    AACATCTTTA TCATCAAGTG CAGCCGAGAG ACAGAGAAGA AATACCCCCA GCCCAAGGGG
    CAGAAGAAGA AGAAAATTGT CAAGTATGGT ATGGGAGGCC TCATTATCCT CTTCCTCATC
    GCCATCATCT GGTTCCCTCT GCTCTTCATG TCACTGATCC GCTCTGTGGT CGGGGTCGTC
    AACCAGCCCA TTGATGTCAC CGTCACCCTC AAGCTAGGCG GCTATGAGCC ACTGTTCACC
    ATGAGCGCCC AGCAGCCATC CATTGTGCCA TTCACACCCC AGGCCTACGA GGAGCTGTCC
    CAGCAGTTTG ACCCCTATCC ACTAGCCATG CAGTTCATTA GCCAGTACAG TCCTGAGGAC
    ATCGTCACTG CACAGATCGA GGGCAGCTCG GGGGCGCTGT GGCGCATCAG CCCACCCAGC
    CGAGCCCAGA TGAAGCAGGA GCTGTACAAC GGCACAGCCG ACATTACACT GCGCTTTACC
    TGGAATTTCC AAAGGGACCT GGCCAAGGGT GGCACTGTGG AGTATACTAA TGAGAAGCAC
    ACCTTGGAGC TGGCCCCCAA CAGTACGGCA CGAAGGCAGC TGGCCCAACT GCTCGAGGGC
    AGACCTGACC AGTCAGTGGT CATTCCCCAT CTCTTCCCCA AGTACATTCG TGCTCCCAAT
    GGGCCTGAAG CCAACCCTGT GAAGCAGCTG CAGCCAGATG AGGAAGAGGA CTACCTTGGT
    GTGCGCATCC AGCTGCGGAG GGAGCAAGTG GGCACAGGGG CCTCTGGGGA GCAAGCGGGC
    ACCAAGGCCT CCGACTTCCT CGAGTGGTGG GTCATCGAGC TGCAGGACTG CAAGGCTGAC
    TGCAACCTGC TGCCCATGGT CATCTTCAGT GACAAGGTCA GCCCACCTAG CCTGGGCTTC
    CTGGCCGGCT ACGGGATTGT GGGGCTGTAC GTCTCCATCG TGCTGGTGGT TGGCAAGTTT
    GTGCGGGGCT TCTTCAGCGA GATCTCTCAC TCCATCATGT TCGAGGAACT GCCGTGTGTG
    GACCGCATCC TCAAGCTGTG CCAGGACATC TTCTTGGTGC GCGAGACCCG GGAGCTGGAG
    CTGGAGGAGG AGCTATACGC CAAGCTCATC TTCCTGTACC GATCTCCAGA GACCATGATT
    AAGTGGACAC GTGAGAGGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu73790
    Clone ID Related Accession (Same CDS sequence) XM_006530954.2
    Accession Version XM_006530954.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGGTATTC TGTCTGTGGT GCACAAGCCT TATGTATGCA TGCTTACAGC CTCCCTGTTA 
    CGCTTCAATG CTCTCTCGCT GGTCTATTTG CTGTTTCTAC TGCTGCTGCC CTGGCTTCCA
    GGCCCCTCAA GACACAGCAT ACCAGGTCAC ACAGGTCGCC TGCTCCGTGC ACTACTCTGC
    CTCAGCCTCC TCTTCCTGGT GGCCCACCTT GCCTTTCAGA TATGCCTACA CACCGTGCCT
    CACCTGGACC AGTTTCTGGG ACAAAACGGT AGCCTTTGGG TGAAGGTGTC TCAACACATA
    GGGGTTACGA GGCTGGACCT GAAGGACATC TTTAACACCA CCAGGCTGGT AGCACCTGAC
    CTGGGAGTGC TGCTGGCGTC CTCCCTTTGC CTTGGCCTCT GTGGACGCCT CACGAGGAAA
    GCCCGGCAGA GTCGGCGCAC CCAGGAGCTG GATGATGACG ATGACGATGA TGACGACGAT
    GAAGACATAG ATGCTGCCCC AGCCGTGGGG CTGAAGGGAG CCCCTGCCCT GGCAACCAAA
    CGCAGGCTGT GGCTGGCCTC CCGCTTCCGG GTCACGGCCC ACTGGCTGCT GATGACCTCT
    GGACGGACGC TGGTCATTGT GTTGCTGGCC CTGGCAGGCA TAGCCCACCC TTCGGCCTTC
    TCCAGCGTCT ACCTGGTGGT GTTCCTGGCC ATCTGCACCT GGTGGTCCTG TCACTTTCCT
    CTCAGCCCCC TGGGCTTTAA CACCCTCTGT GTCATGGTGA GCTGTTTTGG TGCCGGCCAT
    CTCATTTGCC TATACTGCTA TCAGACACCA TTTATCCAGG ACATGTTACC CCCTGGGAAC
    ATCTGGGCCA GGCTATTTGG TCTCAAGAAC TTCGTAGACC TCCCTAACTA CTCCAGCCCC
    AACGCCCTGG TACTCAACAC TAAGCACGCC TGGCCCATCT ATGTGAGTCC CGGAATCCTG
    CTGCTGCTAT ATTACACAGC CACCTCTCTC CTGAAGCTCC ACAAGAGCTG TCCCTCAGAG
    CTGAGGAAGG AGACACCCAG GGAGGATGAG GAGCATGAGC TGGAACTGGA CCACCTGGAG
    CCAGAGCCCC AGGCTAGGGA CGCCACCCAG GGTGAGATGC CCATGACCAC GGAACCTGAC
    CTTGACAACT GCACCGTGCA TGTACTAACC AGCCAGAGCC CTGTCCGCCA GCGTCCAGTT
    CGCCCCAGGC TGGCTGAGCT GAAAGAGATG TCACCGCTAC ATGGCCTGGG CCACCTCATC
    ATGGACCAGA GCTATGTGTG TGCCCTGATT GCCATGATGG TGTGGAGCAT CATGTACCAC
    AGCTGGCTGA CCTTCGTCCT GCTGCTCTGG GCCTGCCTCA TCTGGACGGT GCGTAGCCGC
    CACCAGCTGG CTATGCTCTG CTCGCCCTGC ATCCTGCTGT ATGGGCTGAC GCTCTGCTGC
    CTGAGGTACG TGTGGGCCAT GGAACTTCCT GAGCTGCCCA CCACCCTGGG CCCTGTCAGC
    CTGCACCAGT TGGGACTGGA ACACACACGC TACCCTTGCC TGGACCTCGG TGCCATGCTC
    CTCTATCTGC TCACATTCTG GCTCCTTCTG CGTCAGTTCG TGAAGGAGAA GCTGCTGAAG
    AAGCAAAAGG TGCCTGCGGC GCTTTTGGAG GTCACGGTGG CCGACACTGA GCCCACACAG
    ACCCAGACGC TGCTGCGGAG CCTGGGGGAG CTGGTCACCG GCATCTACGT CAAATACTGG
    ATCTATGTGT GCGCCGGCAT GTTCATTGTG GTCAGCTTCG CCGGCCGCCT GGTGGTCTAC
    AAAATCGTCT ACATGTTCCT CTTCCTGCTG TGCCTCACCT TGTTCCAGGT CTACTACACC
    CTGTGGAGGA AGCTGCTGCG TGTCTTCTGG TGGCTCGTGG TGGCCTATAC AATGCTCGTG
    CTCATCGCTG TGTACACCTT CCAGTTCCAG GACTTCCCCA CCTATTGGCG CAACCTCACG
    GGCTTCACGG ACGAGCAGTT GGGCGACCTG GGCCTGGAGC AGTTCAGTGT GTCGGAGCTC
    TTTTCCAGTA TCCTCATCCC TGGCTTCTTC CTGCTGGCCT GCATCCTGCA GCTGCACTAC
    TTCCACAGAC CGTTCATGCA GCTCACTGAC CTGGAGCACG TGCCGCCACC AGGCACCCGC
    CACCCTCGAT GGGCTCACAG GCAGGATGCA GTGAGCGAGG CCCCTCTGCT TGAGCATCAG
    GAGGAAGAGG AAGTCTTCAG GGAAGATGGG CAGAGCATGG ATGGGCCCCA CCAGGCCACA
    CAGGTCCCTG AGGGTACGGC CAGCAAGTGG GGCCTGGTGG CTGACCGGCT GCTGGACCTA
    GCGGCCAGCT TCTCGGCTGT CCTCACCCGA ATCCAGGTGT TCGTTCGGCG CTTGCTAGAA
    CTTCACGTCT TCAAGCTGGT GGCCCTCTAC ACTGTCTGGG TGGCCCTGAA AGAAGTGTCT
    GTGATGAACC TGCTGCTGGT GGTGCTATGG GCCTTCGCCT TGCCCTATCC GCGCTTCCGG
    CCCATGGCTT CCTGCCTGTC CACCGTGTGG ACCTGTATCA TCATTGTATG CAAGATGCTC
    TATCAGCTCA AGATTGTCAA CCCGCATGAG TACTCCAGCA ACTGCACTGA GCCCTTCCCC
    AACAATACCA ACTTGCAGCC TTTGGAGATC AACCAGTCTT TGCTGTACCG TGGCCCTGTT
    GACCCTGCCA ACTGGTTTGG GGTGCGGAAG GGTTACCCCA ACTTGGGCTA TATCCAGAAC
    CACCTGCAGA TCCTTCTGTT GCTGGTGTTT GAGGCCGTGG TGTACCGGCG CCAAGAGCAC
    TACCGCCGGC AGCACCAGCA GGCCCCTCTG CCCGCCCAGG CTGTGTGCGC AGATGGCACC
    CGCCAGAGGT TGGACCAGGA CCTACTTAGC TGCCTCAAGT ATTTCATCAA CTTCTTCTTC
    TACAAATTCG GGCTGGAGAT CTGCTTCTTG ATGGCCGTGA ATGTGATTGG GCAGCGTATG
    AACTTCATGG TGATCTTACA CGGTTGCTGG TTGGTGGCCA TCCTTACACG CCGGCGCCGT
    GAGGCCATCG CCCGCCTCTG GCCTAACTAC TGTCTGTTCC TCACGCTGTT CCTGCTGTAC
    CAGTACCTGC TGTGTTTGGG CATGCCCCCT GCACTGTGCA TTGACTATCC ATGGCGCTGG
    AGCAAGGCCA TCCCCATGAA TTCCGCCCTC ATCAAGTGGC TGTACCTACC TGACTTCTTC
    AGAGCCCCCA ACTCCACCAA CCTTATCAGT GACTTCCTCC TGCTGCTTTG CGCCTCCCAG
    CAGTGGCAGG TCTTCTCAGC GGAGCGAACG GAGGAGTGGC AACGCATGGC GGGCATCAAC
    ACTGACCACC TGGAGCCCCT GCGTGGGGAG CCCAACCCTA TACCCAACTT CATCCACTGC
    AGGTCCTATC TGGATATGCT GAAGGTGGCC GTCTTCCGCT ACCTGTTCTG GCTGGTGCTC
    GTTGTGGTGT TTGTTGCGGG GGCCACCCGC ATAAGCATCT TCGGGCTGGG GTACCTGCTA
    GCCTGCTTCT ACCTGCTGCT GTTTGGCACT ACCCTGCTGC AGAAGGACAC GCGAGCCCAG
    CTCGTGCTGT GGGACTGCCT CATCCTCTAT AATGTCACTG TCATCATCTC TAAGAATATG
    CTGTCGCTCC TGTCCTGTGT CTTCGTGGAG CAAATGCAGA GCAACTTCTG CTGGGTCATC
    CAGCTCTTCA GCCTCGTGTG CACAGTCAAA GGCTACTATG ATCCCAAAGA GATGATGACC
    AGGGACCGGG ACTGCCTGCT GCCTGTGGAG GAGGCCGGGA TCATCTGGGA CAGTATCTGC
    TTCTTCTTCC TGCTCTTGCA ACGGCGCATC TTTCTCAGCC ACTACTTCCT GCATGTCAGC
    GCTGACCTGA AAGCCACAGC CCTGCAGGCA TCCAGGGGCT TTGCCCTCTA CAATGCAGCC
    AACCTGAAGA GCATCAACTT CCATCGCCAG ATTGAGGAGA AGTCCCTGGC CCAGCTGAAA
    AGACAGATGA AGCGCATCCG TGCCAAACAG GAGAAGTACA GGCAGAGCCA GGCAAGTCGT
    GGCCAACTCC AGTCCAAAGA CCCTCAGGAT CCCAGCCAGG AGCCAGGGCC TGACAGCCCA
    GGGGGCTCCT CCCCGCCACG GAGACAGTGG TGGCGCCCCT GGCTGGACCA CGCCACAGTC
    ATCCACTCTG GCGACTACTT CCTGTTTGAG TCAGATAGCG AGGAGGAAGA GGAGGCCCTA
    CCTGAGGACC CCAGGCCTGC AGCTCAGAGT GCCTTCCAGA TGGCATACCA GGCATGGGTA
    ACCAATGCCC AGACAGTGCT GAGGCAGCGT CGGGAGCGGG CACGGCAGGA GCGGGCAGAG
    CAGCTGGCTT CTGGAGGTGA CTTGAACCCA GATGTGGAAC CAGTAGATGT CCCAGAAGAT
    GAGATGGCAG GCCGTAGCCA CATGATGCAG CGTGTGCTAA GCACCATGCA GTTCCTGTGG
    GTGCTGGGCC AGGCCACGGT AGACGGGCTG ACGCGCTGGC TGCGTGCATT CACGAAGCAC
    CACCGCACCA TGAGCGATGT GCTGTGCGCA GAGCGCTACC TGCTCACCCA GGAGCTTCTT
    CGGGTTGGAG AGGTACGCCG AGGTGTGCTG GACCAGCTTT ATGTGGGTGA AGATGAGGCC
    ACATTGTCAG GTCCCATGGA GACCCGGGAT GGACCCAGCA CAGCCTCAAG TGGGCTGGGA
    GCCGAAGAGC CTTTGAGTAG CATGACAGAC GACACCAGCA GCCCCCTGAG CACAGGCTAT
    AACACCCGCA GTGGCAGTGA GGAGATTGTC ACCGACGCTG GGGACCTCCA GGCTGGGACC
    TCCCTGCACG GCTCCCAAGA GCTTTTAGCC AATGCTCGTA CCCGGATGCG CACGGCCAGC
    GAGCTGCTAC TGGATAGGCG CCTGCATATC CCTGAGCTGG AGGAGGCCGA GCGGTTTGAG
    GCACAGCAGG GCCGGACTCT GCGGCTGCTC AGGGCTGGGT ACCAGTGCGT GGCGGCACAC
    TCGGAGCTGC TCTGTTACTT CATCATCATC CTTAACCACA TGGTGACAGC CTCGGCTGCC
    TCCCTGGTGC TGCCCGTGCT TGTGTTCCTG TGGGCCATGC TGACCATCCC GAGGCCTAGC
    AAGCGCTTTT GGATGACAGC TATCGTCTTC ACTGAGGTCA TGGTGGTCAC CAAATACCTG
    TTCCAGTTCG GCTTCTTCCC CTGGAACAGC TACGTTGTGC TGCGGCGCTA TGAGAACAAG
    CCCTACTTCC CTCCGCGAAT CCTGGGCCTT GAGAAAACGG ACAGCTACAT CAAGTATGAC
    CTGGTGCAGC TCATGGCCCT CTTCTTCCAC CGCTCGCAGC TACTGTGTTA TGGCCTCTGG
    GACCATGAGG AGGATCGCTA TCCCAAGGAC CATTGCAGGA GTAGTGTGAA GGACCGGGAG
    GCCAAGGAAG AGCCAGAAGC TAAGCTGGAA TCGCAGTCTG AGACAGGCAC TGGGCATCCC
    AAGGAGCCAG TGTTGGCCGG TACTCCCAGG GACCACATCC AAGGGAAAGG AAGTATTAGA
    TCCAAGGATG TTATCCAAGA TCCCCCAGAG GACCTTAAGC CCCGGCACAC GAGGCACATC
    AGCATACGCT TCAGGAGGCG CAAGGAGACT CCAGGACCCA AAGGAACAGC AGTCATGGAG
    ACTGAGCACG AGGAGGGAGA AGGAAAAGAA ACTACAGAGA GAAAGAGGCC GCGTCACACT
    CAAGAAAAAT CGAAGTTTCG GGAGAGAATG AAGGCAGCTG GGCGCCGGCT GCAGAGCTTC
    TGTGTGTCAC TGGCCCAGAG CTTCTACCAA CCCTTGCAGC GTTTCTTCCA TGACATTCTG
    CACACAAAGT ACCGGGCGGC CACCGACGTC TACGCCCTCA TGTTCCTGGC CGATATTGTC
    GACATCATCA TCATCATCTT TGGTTTTTGG GCTTTTGGGA AGCACTCTGC AGCCACAGAC
    ATTGCATCCT CGCTGTCAGA TGACCAGGTG CCACAGGCCT TCCTGTTCAT GCTGCTGGTC
    CAGTTTGGCA CCATGGTCAT CGACCGTGCC CTCTACCTGC GCAAGACTGT CCTGGGAAAG
    CTGGCCTTTC AGGTGGTCCT GGTGGTGGCG ATTCACATCT GGATGTTCTT TATCTTACCG
    GCTGTCACTG AGAGGATGTT CAGCCAGAAT GCGGTGGCAC AGCTGTGGTA CTTCGTCAAG
    TGCATTTACT TTGCCCTGTC CGCCTACCAG ATCCGCTGTG GCTACCCCAC CCGTATCTTG
    GGCAACTTCC TCACCAAGAA ATACAACCAT CTAAACCTCT TCCTTTTCCA GGGGTTCCGT
    CTAGTGCCGT TCCTGGTGGA GCTGCGGGCC GTCATGGACT GGGTGTGGAC CGACACCACG
    CTGTCCCTGT CCAACTGGAT GTGTGTGGAA GACATCTATG CCAACATCTT TATCATCAAG
    TGCAGCCGAG AGACAGAGAA GAAATACCCC CAGCCCAAGG GGCAGAAGAA GAAGAAAATT
    GTCAAGTATG GTATGGGAGG CCTCATTATC CTCTTCCTCA TCGCCATCAT CTGGTTCCCT
    CTGCTCTTCA TGTCACTGAT CCGCTCTGTG GTCGGGGTCG TCAACCAGCC CATTGATGTC
    ACCGTCACCC TCAAGCTAGG CGGCTATGAG CCACTGTTCA CCATGAGCGC CCAGCAGCCA
    TCCATTGTGC CATTCACACC CCAGGCCTAC GAGGAGCTGT CCCAGCAGTT TGACCCCTAT
    CCACTAGCCA TGCAGTTCAT TAGCCAGTAC AGTCCTGAGG ACATCGTCAC TGCACAGATC
    GAGGGCAGCT CGGGGGCGCT GTGGCGCATC AGCCCACCCA GCCGAGCCCA GATGAAGCAG
    GAGCTGTACA ACGGCACAGC CGACATTACA CTGCGCTTTA CCTGGAATTT CCAAAGGGAC
    CTGGCCAAGG GTGGCACTGT GGAGTATACT AATGAGAAGC ACACCTTGGA GCTGGCCCCC
    AACAGTACGG CACGAAGGCA GCTGGCCCAA CTGCTCGAGG GCAGACCTGA CCAGTCAGTG
    GTCATTCCCC ATCTCTTCCC CAAGTACATT CGTGCTCCCA ATGGGCCTGA AGCCAACCCT
    GTGAAGCAGC TGCAGCCAGA TGAGGAAGAG GACTACCTTG GTGTGCGCAT CCAGCTGCGG
    AGGGAGCAAG TGGGCACAGG GGCCTCTGGG GAGCAAGCGG GCACCAAGGC CTCCGACTTC
    CTCGAGTGGT GGGTCATCGA GCTGCAGGAC TGCAAGGCTG ACTGCAACCT GCTGCCCATG
    GTCATCTTCA GTGACAAGGT CAGCCCACCT AGCCTGGGCT TCCTGGCCGG CTACGGGATT
    GTGGGGCTGT ACGTCTCCAT CGTGCTGGTG GTTGGCAAGT TTGTGCGGGG CTTCTTCAGC
    GAGATCTCTC ACTCCATCAT GTTCGAGGAA CTGCCGTGTG TGGACCGCAT CCTCAAGCTG
    TGCCAGGACA TCTTCTTGGT GCGCGAGACC CGGGAGCTGG AGCTGGAGGA GGAGCTATAC
    GCCAAGCTCA TCTTCCTGTA CCGATCTCCA GAGACCATGA TTAAGTGGAC ACGTGAGAGG
    GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006531017.2
    CDS16..7641
    Translation

    Target ORF information:

    RefSeq Version XM_006530954.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530954.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGGTATTC TGTCTGTGGT GCACAAGCCT TATGTATGCA TGCTTACAGC CTCCCTGTTA 
    CGCTTCAATG CTCTCTCGCT GGTCTATTTG CTGTTTCTAC TGCTGCTGCC CTGGCTTCCA
    GGCCCCTCAA GACACAGCAT ACCAGGTCAC ACAGGTCGCC TGCTCCGTGC ACTACTCTGC
    CTCAGCCTCC TCTTCCTGGT GGCCCACCTT GCCTTTCAGA TATGCCTACA CACCGTGCCT
    CACCTGGACC AGTTTCTGGG ACAAAACGGT AGCCTTTGGG TGAAGGTGTC TCAACACATA
    GGGGTTACGA GGCTGGACCT GAAGGACATC TTTAACACCA CCAGGCTGGT AGCACCTGAC
    CTGGGAGTGC TGCTGGCGTC CTCCCTTTGC CTTGGCCTCT GTGGACGCCT CACGAGGAAA
    GCCCGGCAGA GTCGGCGCAC CCAGGAGCTG GATGATGACG ATGACGATGA TGACGACGAT
    GAAGACATAG ATGCTGCCCC AGCCGTGGGG CTGAAGGGAG CCCCTGCCCT GGCAACCAAA
    CGCAGGCTGT GGCTGGCCTC CCGCTTCCGG GTCACGGCCC ACTGGCTGCT GATGACCTCT
    GGACGGACGC TGGTCATTGT GTTGCTGGCC CTGGCAGGCA TAGCCCACCC TTCGGCCTTC
    TCCAGCGTCT ACCTGGTGGT GTTCCTGGCC ATCTGCACCT GGTGGTCCTG TCACTTTCCT
    CTCAGCCCCC TGGGCTTTAA CACCCTCTGT GTCATGGTGA GCTGTTTTGG TGCCGGCCAT
    CTCATTTGCC TATACTGCTA TCAGACACCA TTTATCCAGG ACATGTTACC CCCTGGGAAC
    ATCTGGGCCA GGCTATTTGG TCTCAAGAAC TTCGTAGACC TCCCTAACTA CTCCAGCCCC
    AACGCCCTGG TACTCAACAC TAAGCACGCC TGGCCCATCT ATGTGAGTCC CGGAATCCTG
    CTGCTGCTAT ATTACACAGC CACCTCTCTC CTGAAGCTCC ACAAGAGCTG TCCCTCAGAG
    CTGAGGAAGG AGACACCCAG GGAGGATGAG GAGCATGAGC TGGAACTGGA CCACCTGGAG
    CCAGAGCCCC AGGCTAGGGA CGCCACCCAG GGTGAGATGC CCATGACCAC GGAACCTGAC
    CTTGACAACT GCACCGTGCA TGTACTAACC AGCCAGAGCC CTGTCCGCCA GCGTCCAGTT
    CGCCCCAGGC TGGCTGAGCT GAAAGAGATG TCACCGCTAC ATGGCCTGGG CCACCTCATC
    ATGGACCAGA GCTATGTGTG TGCCCTGATT GCCATGATGG TGTGGAGCAT CATGTACCAC
    AGCTGGCTGA CCTTCGTCCT GCTGCTCTGG GCCTGCCTCA TCTGGACGGT GCGTAGCCGC
    CACCAGCTGG CTATGCTCTG CTCGCCCTGC ATCCTGCTGT ATGGGCTGAC GCTCTGCTGC
    CTGAGGTACG TGTGGGCCAT GGAACTTCCT GAGCTGCCCA CCACCCTGGG CCCTGTCAGC
    CTGCACCAGT TGGGACTGGA ACACACACGC TACCCTTGCC TGGACCTCGG TGCCATGCTC
    CTCTATCTGC TCACATTCTG GCTCCTTCTG CGTCAGTTCG TGAAGGAGAA GCTGCTGAAG
    AAGCAAAAGG TGCCTGCGGC GCTTTTGGAG GTCACGGTGG CCGACACTGA GCCCACACAG
    ACCCAGACGC TGCTGCGGAG CCTGGGGGAG CTGGTCACCG GCATCTACGT CAAATACTGG
    ATCTATGTGT GCGCCGGCAT GTTCATTGTG GTCAGCTTCG CCGGCCGCCT GGTGGTCTAC
    AAAATCGTCT ACATGTTCCT CTTCCTGCTG TGCCTCACCT TGTTCCAGGT CTACTACACC
    CTGTGGAGGA AGCTGCTGCG TGTCTTCTGG TGGCTCGTGG TGGCCTATAC AATGCTCGTG
    CTCATCGCTG TGTACACCTT CCAGTTCCAG GACTTCCCCA CCTATTGGCG CAACCTCACG
    GGCTTCACGG ACGAGCAGTT GGGCGACCTG GGCCTGGAGC AGTTCAGTGT GTCGGAGCTC
    TTTTCCAGTA TCCTCATCCC TGGCTTCTTC CTGCTGGCCT GCATCCTGCA GCTGCACTAC
    TTCCACAGAC CGTTCATGCA GCTCACTGAC CTGGAGCACG TGCCGCCACC AGGCACCCGC
    CACCCTCGAT GGGCTCACAG GCAGGATGCA GTGAGCGAGG CCCCTCTGCT TGAGCATCAG
    GAGGAAGAGG AAGTCTTCAG GGAAGATGGG CAGAGCATGG ATGGGCCCCA CCAGGCCACA
    CAGGTCCCTG AGGGTACGGC CAGCAAGTGG GGCCTGGTGG CTGACCGGCT GCTGGACCTA
    GCGGCCAGCT TCTCGGCTGT CCTCACCCGA ATCCAGGTGT TCGTTCGGCG CTTGCTAGAA
    CTTCACGTCT TCAAGCTGGT GGCCCTCTAC ACTGTCTGGG TGGCCCTGAA AGAAGTGTCT
    GTGATGAACC TGCTGCTGGT GGTGCTATGG GCCTTCGCCT TGCCCTATCC GCGCTTCCGG
    CCCATGGCTT CCTGCCTGTC CACCGTGTGG ACCTGTATCA TCATTGTATG CAAGATGCTC
    TATCAGCTCA AGATTGTCAA CCCGCATGAG TACTCCAGCA ACTGCACTGA GCCCTTCCCC
    AACAATACCA ACTTGCAGCC TTTGGAGATC AACCAGTCTT TGCTGTACCG TGGCCCTGTT
    GACCCTGCCA ACTGGTTTGG GGTGCGGAAG GGTTACCCCA ACTTGGGCTA TATCCAGAAC
    CACCTGCAGA TCCTTCTGTT GCTGGTGTTT GAGGCCGTGG TGTACCGGCG CCAAGAGCAC
    TACCGCCGGC AGCACCAGCA GGCCCCTCTG CCCGCCCAGG CTGTGTGCGC AGATGGCACC
    CGCCAGAGGT TGGACCAGGA CCTACTTAGC TGCCTCAAGT ATTTCATCAA CTTCTTCTTC
    TACAAATTCG GGCTGGAGAT CTGCTTCTTG ATGGCCGTGA ATGTGATTGG GCAGCGTATG
    AACTTCATGG TGATCTTACA CGGTTGCTGG TTGGTGGCCA TCCTTACACG CCGGCGCCGT
    GAGGCCATCG CCCGCCTCTG GCCTAACTAC TGTCTGTTCC TCACGCTGTT CCTGCTGTAC
    CAGTACCTGC TGTGTTTGGG CATGCCCCCT GCACTGTGCA TTGACTATCC ATGGCGCTGG
    AGCAAGGCCA TCCCCATGAA TTCCGCCCTC ATCAAGTGGC TGTACCTACC TGACTTCTTC
    AGAGCCCCCA ACTCCACCAA CCTTATCAGT GACTTCCTCC TGCTGCTTTG CGCCTCCCAG
    CAGTGGCAGG TCTTCTCAGC GGAGCGAACG GAGGAGTGGC AACGCATGGC GGGCATCAAC
    ACTGACCACC TGGAGCCCCT GCGTGGGGAG CCCAACCCTA TACCCAACTT CATCCACTGC
    AGGTCCTATC TGGATATGCT GAAGGTGGCC GTCTTCCGCT ACCTGTTCTG GCTGGTGCTC
    GTTGTGGTGT TTGTTGCGGG GGCCACCCGC ATAAGCATCT TCGGGCTGGG GTACCTGCTA
    GCCTGCTTCT ACCTGCTGCT GTTTGGCACT ACCCTGCTGC AGAAGGACAC GCGAGCCCAG
    CTCGTGCTGT GGGACTGCCT CATCCTCTAT AATGTCACTG TCATCATCTC TAAGAATATG
    CTGTCGCTCC TGTCCTGTGT CTTCGTGGAG CAAATGCAGA GCAACTTCTG CTGGGTCATC
    CAGCTCTTCA GCCTCGTGTG CACAGTCAAA GGCTACTATG ATCCCAAAGA GATGATGACC
    AGGGACCGGG ACTGCCTGCT GCCTGTGGAG GAGGCCGGGA TCATCTGGGA CAGTATCTGC
    TTCTTCTTCC TGCTCTTGCA ACGGCGCATC TTTCTCAGCC ACTACTTCCT GCATGTCAGC
    GCTGACCTGA AAGCCACAGC CCTGCAGGCA TCCAGGGGCT TTGCCCTCTA CAATGCAGCC
    AACCTGAAGA GCATCAACTT CCATCGCCAG ATTGAGGAGA AGTCCCTGGC CCAGCTGAAA
    AGACAGATGA AGCGCATCCG TGCCAAACAG GAGAAGTACA GGCAGAGCCA GGCAAGTCGT
    GGCCAACTCC AGTCCAAAGA CCCTCAGGAT CCCAGCCAGG AGCCAGGGCC TGACAGCCCA
    GGGGGCTCCT CCCCGCCACG GAGACAGTGG TGGCGCCCCT GGCTGGACCA CGCCACAGTC
    ATCCACTCTG GCGACTACTT CCTGTTTGAG TCAGATAGCG AGGAGGAAGA GGAGGCCCTA
    CCTGAGGACC CCAGGCCTGC AGCTCAGAGT GCCTTCCAGA TGGCATACCA GGCATGGGTA
    ACCAATGCCC AGACAGTGCT GAGGCAGCGT CGGGAGCGGG CACGGCAGGA GCGGGCAGAG
    CAGCTGGCTT CTGGAGGTGA CTTGAACCCA GATGTGGAAC CAGTAGATGT CCCAGAAGAT
    GAGATGGCAG GCCGTAGCCA CATGATGCAG CGTGTGCTAA GCACCATGCA GTTCCTGTGG
    GTGCTGGGCC AGGCCACGGT AGACGGGCTG ACGCGCTGGC TGCGTGCATT CACGAAGCAC
    CACCGCACCA TGAGCGATGT GCTGTGCGCA GAGCGCTACC TGCTCACCCA GGAGCTTCTT
    CGGGTTGGAG AGGTACGCCG AGGTGTGCTG GACCAGCTTT ATGTGGGTGA AGATGAGGCC
    ACATTGTCAG GTCCCATGGA GACCCGGGAT GGACCCAGCA CAGCCTCAAG TGGGCTGGGA
    GCCGAAGAGC CTTTGAGTAG CATGACAGAC GACACCAGCA GCCCCCTGAG CACAGGCTAT
    AACACCCGCA GTGGCAGTGA GGAGATTGTC ACCGACGCTG GGGACCTCCA GGCTGGGACC
    TCCCTGCACG GCTCCCAAGA GCTTTTAGCC AATGCTCGTA CCCGGATGCG CACGGCCAGC
    GAGCTGCTAC TGGATAGGCG CCTGCATATC CCTGAGCTGG AGGAGGCCGA GCGGTTTGAG
    GCACAGCAGG GCCGGACTCT GCGGCTGCTC AGGGCTGGGT ACCAGTGCGT GGCGGCACAC
    TCGGAGCTGC TCTGTTACTT CATCATCATC CTTAACCACA TGGTGACAGC CTCGGCTGCC
    TCCCTGGTGC TGCCCGTGCT TGTGTTCCTG TGGGCCATGC TGACCATCCC GAGGCCTAGC
    AAGCGCTTTT GGATGACAGC TATCGTCTTC ACTGAGGTCA TGGTGGTCAC CAAATACCTG
    TTCCAGTTCG GCTTCTTCCC CTGGAACAGC TACGTTGTGC TGCGGCGCTA TGAGAACAAG
    CCCTACTTCC CTCCGCGAAT CCTGGGCCTT GAGAAAACGG ACAGCTACAT CAAGTATGAC
    CTGGTGCAGC TCATGGCCCT CTTCTTCCAC CGCTCGCAGC TACTGTGTTA TGGCCTCTGG
    GACCATGAGG AGGATCGCTA TCCCAAGGAC CATTGCAGGA GTAGTGTGAA GGACCGGGAG
    GCCAAGGAAG AGCCAGAAGC TAAGCTGGAA TCGCAGTCTG AGACAGGCAC TGGGCATCCC
    AAGGAGCCAG TGTTGGCCGG TACTCCCAGG GACCACATCC AAGGGAAAGG AAGTATTAGA
    TCCAAGGATG TTATCCAAGA TCCCCCAGAG GACCTTAAGC CCCGGCACAC GAGGCACATC
    AGCATACGCT TCAGGAGGCG CAAGGAGACT CCAGGACCCA AAGGAACAGC AGTCATGGAG
    ACTGAGCACG AGGAGGGAGA AGGAAAAGAA ACTACAGAGA GAAAGAGGCC GCGTCACACT
    CAAGAAAAAT CGAAGTTTCG GGAGAGAATG AAGGCAGCTG GGCGCCGGCT GCAGAGCTTC
    TGTGTGTCAC TGGCCCAGAG CTTCTACCAA CCCTTGCAGC GTTTCTTCCA TGACATTCTG
    CACACAAAGT ACCGGGCGGC CACCGACGTC TACGCCCTCA TGTTCCTGGC CGATATTGTC
    GACATCATCA TCATCATCTT TGGTTTTTGG GCTTTTGGGA AGCACTCTGC AGCCACAGAC
    ATTGCATCCT CGCTGTCAGA TGACCAGGTG CCACAGGCCT TCCTGTTCAT GCTGCTGGTC
    CAGTTTGGCA CCATGGTCAT CGACCGTGCC CTCTACCTGC GCAAGACTGT CCTGGGAAAG
    CTGGCCTTTC AGGTGGTCCT GGTGGTGGCG ATTCACATCT GGATGTTCTT TATCTTACCG
    GCTGTCACTG AGAGGATGTT CAGCCAGAAT GCGGTGGCAC AGCTGTGGTA CTTCGTCAAG
    TGCATTTACT TTGCCCTGTC CGCCTACCAG ATCCGCTGTG GCTACCCCAC CCGTATCTTG
    GGCAACTTCC TCACCAAGAA ATACAACCAT CTAAACCTCT TCCTTTTCCA GGGGTTCCGT
    CTAGTGCCGT TCCTGGTGGA GCTGCGGGCC GTCATGGACT GGGTGTGGAC CGACACCACG
    CTGTCCCTGT CCAACTGGAT GTGTGTGGAA GACATCTATG CCAACATCTT TATCATCAAG
    TGCAGCCGAG AGACAGAGAA GAAATACCCC CAGCCCAAGG GGCAGAAGAA GAAGAAAATT
    GTCAAGTATG GTATGGGAGG CCTCATTATC CTCTTCCTCA TCGCCATCAT CTGGTTCCCT
    CTGCTCTTCA TGTCACTGAT CCGCTCTGTG GTCGGGGTCG TCAACCAGCC CATTGATGTC
    ACCGTCACCC TCAAGCTAGG CGGCTATGAG CCACTGTTCA CCATGAGCGC CCAGCAGCCA
    TCCATTGTGC CATTCACACC CCAGGCCTAC GAGGAGCTGT CCCAGCAGTT TGACCCCTAT
    CCACTAGCCA TGCAGTTCAT TAGCCAGTAC AGTCCTGAGG ACATCGTCAC TGCACAGATC
    GAGGGCAGCT CGGGGGCGCT GTGGCGCATC AGCCCACCCA GCCGAGCCCA GATGAAGCAG
    GAGCTGTACA ACGGCACAGC CGACATTACA CTGCGCTTTA CCTGGAATTT CCAAAGGGAC
    CTGGCCAAGG GTGGCACTGT GGAGTATACT AATGAGAAGC ACACCTTGGA GCTGGCCCCC
    AACAGTACGG CACGAAGGCA GCTGGCCCAA CTGCTCGAGG GCAGACCTGA CCAGTCAGTG
    GTCATTCCCC ATCTCTTCCC CAAGTACATT CGTGCTCCCA ATGGGCCTGA AGCCAACCCT
    GTGAAGCAGC TGCAGCCAGA TGAGGAAGAG GACTACCTTG GTGTGCGCAT CCAGCTGCGG
    AGGGAGCAAG TGGGCACAGG GGCCTCTGGG GAGCAAGCGG GCACCAAGGC CTCCGACTTC
    CTCGAGTGGT GGGTCATCGA GCTGCAGGAC TGCAAGGCTG ACTGCAACCT GCTGCCCATG
    GTCATCTTCA GTGACAAGGT CAGCCCACCT AGCCTGGGCT TCCTGGCCGG CTACGGGATT
    GTGGGGCTGT ACGTCTCCAT CGTGCTGGTG GTTGGCAAGT TTGTGCGGGG CTTCTTCAGC
    GAGATCTCTC ACTCCATCAT GTTCGAGGAA CTGCCGTGTG TGGACCGCAT CCTCAAGCTG
    TGCCAGGACA TCTTCTTGGT GCGCGAGACC CGGGAGCTGG AGCTGGAGGA GGAGCTATAC
    GCCAAGCTCA TCTTCCTGTA CCGATCTCCA GAGACCATGA TTAAGTGGAC ACGTGAGAGG
    GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu73791
    Clone ID Related Accession (Same CDS sequence) XM_006530956.2
    Accession Version XM_006530956.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGGTATTC TGTCTGTGGT GCACAAGCCT TATGTATGCA TGCTTACAGC CTCCCTGTTA 
    CGCTTCAATG CTCTCTCGCT GGTCTATTTG CTGTTTCTAC TGCTGCTGCC CTGGCTTCCA
    GGCCCCTCAA GACACAGCAT ACCAGGTCAC ACAGGTCGCC TGCTCCGTGC ACTACTCTGC
    CTCAGCCTCC TCTTCCTGGT GGCCCACCTT GCCTTTCAGA TATGCCTACA CACCGTGCCT
    CACCTGGACC AGTTTCTGGG ACAAAACGGT AGCCTTTGGG TGAAGGTGTC TCAACACATA
    GGGGTTACGA GGCTGGACCT GAAGGACATC TTTAACACCA CCAGGCTGGT AGCACCTGAC
    CTGGGAGTGC TGCTGGCGTC CTCCCTTTGC CTTGGCCTCT GTGGACGCCT CACGAGGAAA
    GCCCGGCAGA GTCGGCGCAC CCAGGAGCTG CAGGATGATG ACGATGACGA TGATGACGAC
    GATGAAGACA TAGATGCTGC CCCAGCCGTG GGGCTGAAGG GAGCCCCTGC CCTGGCAACC
    AAACGCAGGC TGTGGCTGGC CTCCCGCTTC CGGGTCACGG CCCACTGGCT GCTGATGACC
    TCTGGACGGA CGCTGGTCAT TGTGTTGCTG GCCCTGGCAG GCATAGCCCA CCCTTCGGCC
    TTCTCCAGCG TCTACCTGGT GGTGTTCCTG GCCATCTGCA CCTGGTGGTC CTGTCACTTT
    CCTCTCAGCC CCCTGGGCTT TAACACCCTC TGTGTCATGG TGAGCTGTTT TGGTGCCGGC
    CATCTCATTT GCCTATACTG CTATCAGACA CCATTTATCC AGGACATGTT ACCCCCTGGG
    AACATCTGGG CCAGGCTATT TGGTCTCAAG AACTTCGTAG ACCTCCCTAA CTACTCCAGC
    CCCAACGCCC TGGTACTCAA CACTAAGCAC GCCTGGCCCA TCTATGTGAG TCCCGGAATC
    CTGCTGCTGC TATATTACAC AGCCACCTCT CTCCTGAAGC TCCACAAGAG CTGTCCCTCA
    GAGCTGAGGA AGGAGACACC CAGGGAGGAT GAGGAGCATG AGCTGGAACT GGACCACCTG
    GAGCCAGAGC CCCAGGCTAG GGACGCCACC CAGGGTGAGA TGCCCATGAC CACGGAACCT
    GACCTTGACA ACTGCACCGT GCATGTACTA ACCAGCCAGA GCCCTGTCCG CCAGCGTCCA
    GTTCGCCCCA GGCTGGCTGA GCTGAAAGAG ATGTCACCGC TACATGGCCT GGGCCACCTC
    ATCATGGACC AGAGCTATGT GTGTGCCCTG ATTGCCATGA TGGTGTGGAG CATCATGTAC
    CACAGCTGGC TGACCTTCGT CCTGCTGCTC TGGGCCTGCC TCATCTGGAC GGTGCGTAGC
    CGCCACCAGC TGGCTATGCT CTGCTCGCCC TGCATCCTGC TGTATGGGCT GACGCTCTGC
    TGCCTGAGGT ACGTGTGGGC CATGGAACTT CCTGAGCTGC CCACCACCCT GGGCCCTGTC
    AGCCTGCACC AGTTGGGACT GGAACACACA CGCTACCCTT GCCTGGACCT CGGTGCCATG
    CTCCTCTATC TGCTCACATT CTGGCTCCTT CTGCGTCAGT TCGTGAAGGA GAAGCTGCTG
    AAGAAGCAAA AGGTGCCTGC GGCGCTTTTG GAGGTCACGG TGGCCGACAC TGAGCCCACA
    CAGACCCAGA CGCTGCTGCG GAGCCTGGGG GAGCTGGTCA CCGGCATCTA CGTCAAATAC
    TGGATCTATG TGTGCGCCGG CATGTTCATT GTGGTCAGCT TCGCCGGCCG CCTGGTGGTC
    TACAAAATCG TCTACATGTT CCTCTTCCTG CTGTGCCTCA CCTTGTTCCA GGTCTACTAC
    ACCCTGTGGA GGAAGCTGCT GCGTGTCTTC TGGTGGCTCG TGGTGGCCTA TACAATGCTC
    GTGCTCATCG CTGTGTACAC CTTCCAGTTC CAGGACTTCC CCACCTATTG GCGCAACCTC
    ACGGGCTTCA CGGACGAGCA GTTGGGCGAC CTGGGCCTGG AGCAGTTCAG TGTGTCGGAG
    CTCTTTTCCA GTATCCTCAT CCCTGGCTTC TTCCTGCTGG CCTGCATCCT GCAGCTGCAC
    TACTTCCACA GACCGTTCAT GCAGCTCACT GACCTGGAGC ACGTGCCGCC ACCAGGCACC
    CGCCACCCTC GATGGGCTCA CAGGCAGGAT GCAGTGAGCG AGGCCCCTCT GCTTGAGCAT
    CAGGAGGAAG AGGAAGTCTT CAGGGAAGAT GGGCAGAGCA TGGATGGGCC CCACCAGGCC
    ACACAGGTCC CTGAGGGTAC GGCCAGCAAG TGGGGCCTGG TGGCTGACCG GCTGCTGGAC
    CTAGCGGCCA GCTTCTCGGC TGTCCTCACC CGAATCCAGG TGTTCGTTCG GCGCTTGCTA
    GAACTTCACG TCTTCAAGCT GGTGGCCCTC TACACTGTCT GGGTGGCCCT GAAAGAAGTG
    TCTGTGATGA ACCTGCTGCT GGTGGTGCTA TGGGCCTTCG CCTTGCCCTA TCCGCGCTTC
    CGGCCCATGG CTTCCTGCCT GTCCACCGTG TGGACCTGTA TCATCATTGT ATGCAAGATG
    CTCTATCAGC TCAAGATTGT CAACCCGCAT GAGTACTCCA GCAACTGCAC TGAGCCCTTC
    CCCAACAATA CCAACTTGCA GCCTTTGGAG ATCAACCAGT CTTTGCTGTA CCGTGGCCCT
    GTTGACCCTG CCAACTGGTT TGGGGTGCGG AAGGGTTACC CCAACTTGGG CTATATCCAG
    AACCACCTGC AGATCCTTCT GTTGCTGGTG TTTGAGGCCG TGGTGTACCG GCGCCAAGAG
    CACTACCGCC GGCAGCACCA GCAGGCCCCT CTGCCCGCCC AGGCTGTGTG CGCAGATGGC
    ACCCGCCAGA GGTTGGACCA GGACCTACTT AGCTGCCTCA AGTATTTCAT CAACTTCTTC
    TTCTACAAAT TCGGGCTGGA GATCTGCTTC TTGATGGCCG TGAATGTGAT TGGGCAGCGT
    ATGAACTTCA TGGTGATCTT ACACGGTTGC TGGTTGGTGG CCATCCTTAC ACGCCGGCGC
    CGTGAGGCCA TCGCCCGCCT CTGGCCTAAC TACTGTCTGT TCCTCACGCT GTTCCTGCTG
    TACCAGTACC TGCTGTGTTT GGGCATGCCC CCTGCACTGT GCATTGACTA TCCATGGCGC
    TGGAGCAAGG CCATCCCCAT GAATTCCGCC CTCATCAAGT GGCTGTACCT ACCTGACTTC
    TTCAGAGCCC CCAACTCCAC CAACCTTATC AGTGACTTCC TCCTGCTGCT TTGCGCCTCC
    CAGCAGTGGC AGGTCTTCTC AGCGGAGCGA ACGGAGGAGT GGCAACGCAT GGCGGGCATC
    AACACTGACC ACCTGGAGCC CCTGCGTGGG GAGCCCAACC CTATACCCAA CTTCATCCAC
    TGCAGGTCCT ATCTGGATAT GCTGAAGGTG GCCGTCTTCC GCTACCTGTT CTGGCTGGTG
    CTCGTTGTGG TGTTTGTTGC GGGGGCCACC CGCATAAGCA TCTTCGGGCT GGGGTACCTG
    CTAGCCTGCT TCTACCTGCT GCTGTTTGGC ACTACCCTGC TGCAGAAGGA CACGCGAGCC
    CAGCTCGTGC TGTGGGACTG CCTCATCCTC TATAATGTCA CTGTCATCAT CTCTAAGAAT
    ATGCTGTCGC TCCTGTCCTG TGTCTTCGTG GAGCAAATGC AGAGCAACTT CTGCTGGGTC
    ATCCAGCTCT TCAGCCTCGT GTGCACAGTC AAAGGCTACT ATGATCCCAA AGAGATGATG
    ACCAGGGACC GGGACTGCCT GCTGCCTGTG GAGGAGGCCG GGATCATCTG GGACAGTATC
    TGCTTCTTCT TCCTGCTCTT GCAACGGCGC ATCTTTCTCA GCCACTACTT CCTGCATGTC
    AGCGCTGACC TGAAAGCCAC AGCCCTGCAG GCATCCAGGG GCTTTGCCCT CTACAATGCA
    GCCAACCTGA AGAGCATCAA CTTCCATCGC CAGATTGAGG AGAAGTCCCT GGCCCAGCTG
    AAAAGACAGA TGAAGCGCAT CCGTGCCAAA CAGGAGAAGT ACAGGCAGAG CCAGGCAAGT
    CGTGGCCAAC TCCAGTCCAA AGACCCTCAG GATCCCAGCC AGGAGCCAGT CATCCACTCT
    GGCGACTACT TCCTGTTTGA GTCAGATAGC GAGGAGGAAG AGGAGGCCCT ACCTGAGGAC
    CCCAGGCCTG CAGCTCAGAG TGCCTTCCAG ATGGCATACC AGGCATGGGT AACCAATGCC
    CAGACAGTGC TGAGGCAGCG TCGGGAGCGG GCACGGCAGG AGCGGGCAGA GCAGCTGGCT
    TCTGGAGGTG ACTTGAACCC AGATGTGGAA CCAGTAGATG TCCCAGAAGA TGAGATGGCA
    GGCCGTAGCC ACATGATGCA GCGTGTGCTA AGCACCATGC AGTTCCTGTG GGTGCTGGGC
    CAGGCCACGG TAGACGGGCT GACGCGCTGG CTGCGTGCAT TCACGAAGCA CCACCGCACC
    ATGAGCGATG TGCTGTGCGC AGAGCGCTAC CTGCTCACCC AGGAGCTTCT TCGGGTTGGA
    GAGGTACGCC GAGGTGTGCT GGACCAGCTT TATGTGGGTG AAGATGAGGC CACATTGTCA
    GGTCCCATGG AGACCCGGGA TGGACCCAGC ACAGCCTCAA GTGGGCTGGG AGCCGAAGAG
    CCTTTGAGTA GCATGACAGA CGACACCAGC AGCCCCCTGA GCACAGGCTA TAACACCCGC
    AGTGGCAGTG AGGAGATTGT CACCGACGCT GGGGACCTCC AGGCTGGGAC CTCCCTGCAC
    GGCTCCCAAG AGCTTTTAGC CAATGCTCGT ACCCGGATGC GCACGGCCAG CGAGCTGCTA
    CTGGATAGGC GCCTGCATAT CCCTGAGCTG GAGGAGGCCG AGCGGTTTGA GGCACAGCAG
    GGCCGGACTC TGCGGCTGCT CAGGGCTGGG TACCAGTGCG TGGCGGCACA CTCGGAGCTG
    CTCTGTTACT TCATCATCAT CCTTAACCAC ATGGTGACAG CCTCGGCTGC CTCCCTGGTG
    CTGCCCGTGC TTGTGTTCCT GTGGGCCATG CTGACCATCC CGAGGCCTAG CAAGCGCTTT
    TGGATGACAG CTATCGTCTT CACTGAGGTC ATGGTGGTCA CCAAATACCT GTTCCAGTTC
    GGCTTCTTCC CCTGGAACAG CTACGTTGTG CTGCGGCGCT ATGAGAACAA GCCCTACTTC
    CCTCCGCGAA TCCTGGGCCT TGAGAAAACG GACAGCTACA TCAAGTATGA CCTGGTGCAG
    CTCATGGCCC TCTTCTTCCA CCGCTCGCAG CTACTGTGTT ATGGCCTCTG GGACCATGAG
    GAGGATCGCT ATCCCAAGGA CCATTGCAGG AGTAGTGTGA AGGACCGGGA GGCCAAGGAA
    GAGCCAGAAG CTAAGCTGGA ATCGCAGTCT GAGACAGGCA CTGGGCATCC CAAGGAGCCA
    GTGTTGGCCG GTACTCCCAG GGACCACATC CAAGGGAAAG GAAGTATTAG ATCCAAGGAT
    GTTATCCAAG ATCCCCCAGA GGACCTTAAG CCCCGGCACA CGAGGCACAT CAGCATACGC
    TTCAGGAGGC GCAAGGAGAC TCCAGGACCC AAAGGAACAG CAGTCATGGA GACTGAGCAC
    GAGGAGGGAG AAGGAAAAGA AACTACAGAG AGAAAGAGGC CGCGTCACAC TCAAGAAAAA
    TCGAAGTTTC GGGAGAGAAT GAAGGCAGCT GGGCGCCGGC TGCAGAGCTT CTGTGTGTCA
    CTGGCCCAGA GCTTCTACCA ACCCTTGCAG CGTTTCTTCC ATGACATTCT GCACACAAAG
    TACCGGGCGG CCACCGACGT CTACGCCCTC ATGTTCCTGG CCGATATTGT CGACATCATC
    ATCATCATCT TTGGTTTTTG GGCTTTTGGG AAGCACTCTG CAGCCACAGA CATTGCATCC
    TCGCTGTCAG ATGACCAGGT GCCACAGGCC TTCCTGTTCA TGCTGCTGGT CCAGTTTGGC
    ACCATGGTCA TCGACCGTGC CCTCTACCTG CGCAAGACTG TCCTGGGAAA GCTGGCCTTT
    CAGGTGGTCC TGGTGGTGGC GATTCACATC TGGATGTTCT TTATCTTACC GGCTGTCACT
    GAGAGGATGT TCAGCCAGAA TGCGGTGGCA CAGCTGTGGT ACTTCGTCAA GTGCATTTAC
    TTTGCCCTGT CCGCCTACCA GATCCGCTGT GGCTACCCCA CCCGTATCTT GGGCAACTTC
    CTCACCAAGA AATACAACCA TCTAAACCTC TTCCTTTTCC AGGGGTTCCG TCTAGTGCCG
    TTCCTGGTGG AGCTGCGGGC CGTCATGGAC TGGGTGTGGA CCGACACCAC GCTGTCCCTG
    TCCAACTGGA TGTGTGTGGA AGACATCTAT GCCAACATCT TTATCATCAA GTGCAGCCGA
    GAGACAGAGA AGAAATACCC CCAGCCCAAG GGGCAGAAGA AGAAGAAAAT TGTCAAGTAT
    GGTATGGGAG GCCTCATTAT CCTCTTCCTC ATCGCCATCA TCTGGTTCCC TCTGCTCTTC
    ATGTCACTGA TCCGCTCTGT GGTCGGGGTC GTCAACCAGC CCATTGATGT CACCGTCACC
    CTCAAGCTAG GCGGCTATGA GCCACTGTTC ACCATGAGCG CCCAGCAGCC ATCCATTGTG
    CCATTCACAC CCCAGGCCTA CGAGGAGCTG TCCCAGCAGT TTGACCCCTA TCCACTAGCC
    ATGCAGTTCA TTAGCCAGTA CAGTCCTGAG GACATCGTCA CTGCACAGAT CGAGGGCAGC
    TCGGGGGCGC TGTGGCGCAT CAGCCCACCC AGCCGAGCCC AGATGAAGCA GGAGCTGTAC
    AACGGCACAG CCGACATTAC ACTGCGCTTT ACCTGGAATT TCCAAAGGGA CCTGGCCAAG
    GGTGGCACTG TGGAGTATAC TAATGAGAAG CACACCTTGG AGCTGGCCCC CAACAGTACG
    GCACGAAGGC AGCTGGCCCA ACTGCTCGAG GGCAGACCTG ACCAGTCAGT GGTCATTCCC
    CATCTCTTCC CCAAGTACAT TCGTGCTCCC AATGGGCCTG AAGCCAACCC TGTGAAGCAG
    CTGCAGCCAG ATGAGGAAGA GGACTACCTT GGTGTGCGCA TCCAGCTGCG GAGGGAGCAA
    GTGGGCACAG GGGCCTCTGG GGAGCAAGCG GGCACCAAGG CCTCCGACTT CCTCGAGTGG
    TGGGTCATCG AGCTGCAGGA CTGCAAGGCT GACTGCAACC TGCTGCCCAT GGTCATCTTC
    AGTGACAAGG TCAGCCCACC TAGCCTGGGC TTCCTGGCCG GCTACGGGAT TGTGGGGCTG
    TACGTCTCCA TCGTGCTGGT GGTTGGCAAG TTTGTGCGGG GCTTCTTCAG CGAGATCTCT
    CACTCCATCA TGTTCGAGGA ACTGCCGTGT GTGGACCGCA TCCTCAAGCT GTGCCAGGAC
    ATCTTCTTGG TGCGCGAGAC CCGGGAGCTG GAGCTGGAGG AGGAGCTATA CGCCAAGCTC
    ATCTTCCTGT ACCGATCTCC AGAGACCATG ATTAAGTGGA CACGTGAGAG GGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006531019.2
    CDS16..7572
    Translation

    Target ORF information:

    RefSeq Version XM_006530956.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530956.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGGTATTC TGTCTGTGGT GCACAAGCCT TATGTATGCA TGCTTACAGC CTCCCTGTTA 
    CGCTTCAATG CTCTCTCGCT GGTCTATTTG CTGTTTCTAC TGCTGCTGCC CTGGCTTCCA
    GGCCCCTCAA GACACAGCAT ACCAGGTCAC ACAGGTCGCC TGCTCCGTGC ACTACTCTGC
    CTCAGCCTCC TCTTCCTGGT GGCCCACCTT GCCTTTCAGA TATGCCTACA CACCGTGCCT
    CACCTGGACC AGTTTCTGGG ACAAAACGGT AGCCTTTGGG TGAAGGTGTC TCAACACATA
    GGGGTTACGA GGCTGGACCT GAAGGACATC TTTAACACCA CCAGGCTGGT AGCACCTGAC
    CTGGGAGTGC TGCTGGCGTC CTCCCTTTGC CTTGGCCTCT GTGGACGCCT CACGAGGAAA
    GCCCGGCAGA GTCGGCGCAC CCAGGAGCTG CAGGATGATG ACGATGACGA TGATGACGAC
    GATGAAGACA TAGATGCTGC CCCAGCCGTG GGGCTGAAGG GAGCCCCTGC CCTGGCAACC
    AAACGCAGGC TGTGGCTGGC CTCCCGCTTC CGGGTCACGG CCCACTGGCT GCTGATGACC
    TCTGGACGGA CGCTGGTCAT TGTGTTGCTG GCCCTGGCAG GCATAGCCCA CCCTTCGGCC
    TTCTCCAGCG TCTACCTGGT GGTGTTCCTG GCCATCTGCA CCTGGTGGTC CTGTCACTTT
    CCTCTCAGCC CCCTGGGCTT TAACACCCTC TGTGTCATGG TGAGCTGTTT TGGTGCCGGC
    CATCTCATTT GCCTATACTG CTATCAGACA CCATTTATCC AGGACATGTT ACCCCCTGGG
    AACATCTGGG CCAGGCTATT TGGTCTCAAG AACTTCGTAG ACCTCCCTAA CTACTCCAGC
    CCCAACGCCC TGGTACTCAA CACTAAGCAC GCCTGGCCCA TCTATGTGAG TCCCGGAATC
    CTGCTGCTGC TATATTACAC AGCCACCTCT CTCCTGAAGC TCCACAAGAG CTGTCCCTCA
    GAGCTGAGGA AGGAGACACC CAGGGAGGAT GAGGAGCATG AGCTGGAACT GGACCACCTG
    GAGCCAGAGC CCCAGGCTAG GGACGCCACC CAGGGTGAGA TGCCCATGAC CACGGAACCT
    GACCTTGACA ACTGCACCGT GCATGTACTA ACCAGCCAGA GCCCTGTCCG CCAGCGTCCA
    GTTCGCCCCA GGCTGGCTGA GCTGAAAGAG ATGTCACCGC TACATGGCCT GGGCCACCTC
    ATCATGGACC AGAGCTATGT GTGTGCCCTG ATTGCCATGA TGGTGTGGAG CATCATGTAC
    CACAGCTGGC TGACCTTCGT CCTGCTGCTC TGGGCCTGCC TCATCTGGAC GGTGCGTAGC
    CGCCACCAGC TGGCTATGCT CTGCTCGCCC TGCATCCTGC TGTATGGGCT GACGCTCTGC
    TGCCTGAGGT ACGTGTGGGC CATGGAACTT CCTGAGCTGC CCACCACCCT GGGCCCTGTC
    AGCCTGCACC AGTTGGGACT GGAACACACA CGCTACCCTT GCCTGGACCT CGGTGCCATG
    CTCCTCTATC TGCTCACATT CTGGCTCCTT CTGCGTCAGT TCGTGAAGGA GAAGCTGCTG
    AAGAAGCAAA AGGTGCCTGC GGCGCTTTTG GAGGTCACGG TGGCCGACAC TGAGCCCACA
    CAGACCCAGA CGCTGCTGCG GAGCCTGGGG GAGCTGGTCA CCGGCATCTA CGTCAAATAC
    TGGATCTATG TGTGCGCCGG CATGTTCATT GTGGTCAGCT TCGCCGGCCG CCTGGTGGTC
    TACAAAATCG TCTACATGTT CCTCTTCCTG CTGTGCCTCA CCTTGTTCCA GGTCTACTAC
    ACCCTGTGGA GGAAGCTGCT GCGTGTCTTC TGGTGGCTCG TGGTGGCCTA TACAATGCTC
    GTGCTCATCG CTGTGTACAC CTTCCAGTTC CAGGACTTCC CCACCTATTG GCGCAACCTC
    ACGGGCTTCA CGGACGAGCA GTTGGGCGAC CTGGGCCTGG AGCAGTTCAG TGTGTCGGAG
    CTCTTTTCCA GTATCCTCAT CCCTGGCTTC TTCCTGCTGG CCTGCATCCT GCAGCTGCAC
    TACTTCCACA GACCGTTCAT GCAGCTCACT GACCTGGAGC ACGTGCCGCC ACCAGGCACC
    CGCCACCCTC GATGGGCTCA CAGGCAGGAT GCAGTGAGCG AGGCCCCTCT GCTTGAGCAT
    CAGGAGGAAG AGGAAGTCTT CAGGGAAGAT GGGCAGAGCA TGGATGGGCC CCACCAGGCC
    ACACAGGTCC CTGAGGGTAC GGCCAGCAAG TGGGGCCTGG TGGCTGACCG GCTGCTGGAC
    CTAGCGGCCA GCTTCTCGGC TGTCCTCACC CGAATCCAGG TGTTCGTTCG GCGCTTGCTA
    GAACTTCACG TCTTCAAGCT GGTGGCCCTC TACACTGTCT GGGTGGCCCT GAAAGAAGTG
    TCTGTGATGA ACCTGCTGCT GGTGGTGCTA TGGGCCTTCG CCTTGCCCTA TCCGCGCTTC
    CGGCCCATGG CTTCCTGCCT GTCCACCGTG TGGACCTGTA TCATCATTGT ATGCAAGATG
    CTCTATCAGC TCAAGATTGT CAACCCGCAT GAGTACTCCA GCAACTGCAC TGAGCCCTTC
    CCCAACAATA CCAACTTGCA GCCTTTGGAG ATCAACCAGT CTTTGCTGTA CCGTGGCCCT
    GTTGACCCTG CCAACTGGTT TGGGGTGCGG AAGGGTTACC CCAACTTGGG CTATATCCAG
    AACCACCTGC AGATCCTTCT GTTGCTGGTG TTTGAGGCCG TGGTGTACCG GCGCCAAGAG
    CACTACCGCC GGCAGCACCA GCAGGCCCCT CTGCCCGCCC AGGCTGTGTG CGCAGATGGC
    ACCCGCCAGA GGTTGGACCA GGACCTACTT AGCTGCCTCA AGTATTTCAT CAACTTCTTC
    TTCTACAAAT TCGGGCTGGA GATCTGCTTC TTGATGGCCG TGAATGTGAT TGGGCAGCGT
    ATGAACTTCA TGGTGATCTT ACACGGTTGC TGGTTGGTGG CCATCCTTAC ACGCCGGCGC
    CGTGAGGCCA TCGCCCGCCT CTGGCCTAAC TACTGTCTGT TCCTCACGCT GTTCCTGCTG
    TACCAGTACC TGCTGTGTTT GGGCATGCCC CCTGCACTGT GCATTGACTA TCCATGGCGC
    TGGAGCAAGG CCATCCCCAT GAATTCCGCC CTCATCAAGT GGCTGTACCT ACCTGACTTC
    TTCAGAGCCC CCAACTCCAC CAACCTTATC AGTGACTTCC TCCTGCTGCT TTGCGCCTCC
    CAGCAGTGGC AGGTCTTCTC AGCGGAGCGA ACGGAGGAGT GGCAACGCAT GGCGGGCATC
    AACACTGACC ACCTGGAGCC CCTGCGTGGG GAGCCCAACC CTATACCCAA CTTCATCCAC
    TGCAGGTCCT ATCTGGATAT GCTGAAGGTG GCCGTCTTCC GCTACCTGTT CTGGCTGGTG
    CTCGTTGTGG TGTTTGTTGC GGGGGCCACC CGCATAAGCA TCTTCGGGCT GGGGTACCTG
    CTAGCCTGCT TCTACCTGCT GCTGTTTGGC ACTACCCTGC TGCAGAAGGA CACGCGAGCC
    CAGCTCGTGC TGTGGGACTG CCTCATCCTC TATAATGTCA CTGTCATCAT CTCTAAGAAT
    ATGCTGTCGC TCCTGTCCTG TGTCTTCGTG GAGCAAATGC AGAGCAACTT CTGCTGGGTC
    ATCCAGCTCT TCAGCCTCGT GTGCACAGTC AAAGGCTACT ATGATCCCAA AGAGATGATG
    ACCAGGGACC GGGACTGCCT GCTGCCTGTG GAGGAGGCCG GGATCATCTG GGACAGTATC
    TGCTTCTTCT TCCTGCTCTT GCAACGGCGC ATCTTTCTCA GCCACTACTT CCTGCATGTC
    AGCGCTGACC TGAAAGCCAC AGCCCTGCAG GCATCCAGGG GCTTTGCCCT CTACAATGCA
    GCCAACCTGA AGAGCATCAA CTTCCATCGC CAGATTGAGG AGAAGTCCCT GGCCCAGCTG
    AAAAGACAGA TGAAGCGCAT CCGTGCCAAA CAGGAGAAGT ACAGGCAGAG CCAGGCAAGT
    CGTGGCCAAC TCCAGTCCAA AGACCCTCAG GATCCCAGCC AGGAGCCAGT CATCCACTCT
    GGCGACTACT TCCTGTTTGA GTCAGATAGC GAGGAGGAAG AGGAGGCCCT ACCTGAGGAC
    CCCAGGCCTG CAGCTCAGAG TGCCTTCCAG ATGGCATACC AGGCATGGGT AACCAATGCC
    CAGACAGTGC TGAGGCAGCG TCGGGAGCGG GCACGGCAGG AGCGGGCAGA GCAGCTGGCT
    TCTGGAGGTG ACTTGAACCC AGATGTGGAA CCAGTAGATG TCCCAGAAGA TGAGATGGCA
    GGCCGTAGCC ACATGATGCA GCGTGTGCTA AGCACCATGC AGTTCCTGTG GGTGCTGGGC
    CAGGCCACGG TAGACGGGCT GACGCGCTGG CTGCGTGCAT TCACGAAGCA CCACCGCACC
    ATGAGCGATG TGCTGTGCGC AGAGCGCTAC CTGCTCACCC AGGAGCTTCT TCGGGTTGGA
    GAGGTACGCC GAGGTGTGCT GGACCAGCTT TATGTGGGTG AAGATGAGGC CACATTGTCA
    GGTCCCATGG AGACCCGGGA TGGACCCAGC ACAGCCTCAA GTGGGCTGGG AGCCGAAGAG
    CCTTTGAGTA GCATGACAGA CGACACCAGC AGCCCCCTGA GCACAGGCTA TAACACCCGC
    AGTGGCAGTG AGGAGATTGT CACCGACGCT GGGGACCTCC AGGCTGGGAC CTCCCTGCAC
    GGCTCCCAAG AGCTTTTAGC CAATGCTCGT ACCCGGATGC GCACGGCCAG CGAGCTGCTA
    CTGGATAGGC GCCTGCATAT CCCTGAGCTG GAGGAGGCCG AGCGGTTTGA GGCACAGCAG
    GGCCGGACTC TGCGGCTGCT CAGGGCTGGG TACCAGTGCG TGGCGGCACA CTCGGAGCTG
    CTCTGTTACT TCATCATCAT CCTTAACCAC ATGGTGACAG CCTCGGCTGC CTCCCTGGTG
    CTGCCCGTGC TTGTGTTCCT GTGGGCCATG CTGACCATCC CGAGGCCTAG CAAGCGCTTT
    TGGATGACAG CTATCGTCTT CACTGAGGTC ATGGTGGTCA CCAAATACCT GTTCCAGTTC
    GGCTTCTTCC CCTGGAACAG CTACGTTGTG CTGCGGCGCT ATGAGAACAA GCCCTACTTC
    CCTCCGCGAA TCCTGGGCCT TGAGAAAACG GACAGCTACA TCAAGTATGA CCTGGTGCAG
    CTCATGGCCC TCTTCTTCCA CCGCTCGCAG CTACTGTGTT ATGGCCTCTG GGACCATGAG
    GAGGATCGCT ATCCCAAGGA CCATTGCAGG AGTAGTGTGA AGGACCGGGA GGCCAAGGAA
    GAGCCAGAAG CTAAGCTGGA ATCGCAGTCT GAGACAGGCA CTGGGCATCC CAAGGAGCCA
    GTGTTGGCCG GTACTCCCAG GGACCACATC CAAGGGAAAG GAAGTATTAG ATCCAAGGAT
    GTTATCCAAG ATCCCCCAGA GGACCTTAAG CCCCGGCACA CGAGGCACAT CAGCATACGC
    TTCAGGAGGC GCAAGGAGAC TCCAGGACCC AAAGGAACAG CAGTCATGGA GACTGAGCAC
    GAGGAGGGAG AAGGAAAAGA AACTACAGAG AGAAAGAGGC CGCGTCACAC TCAAGAAAAA
    TCGAAGTTTC GGGAGAGAAT GAAGGCAGCT GGGCGCCGGC TGCAGAGCTT CTGTGTGTCA
    CTGGCCCAGA GCTTCTACCA ACCCTTGCAG CGTTTCTTCC ATGACATTCT GCACACAAAG
    TACCGGGCGG CCACCGACGT CTACGCCCTC ATGTTCCTGG CCGATATTGT CGACATCATC
    ATCATCATCT TTGGTTTTTG GGCTTTTGGG AAGCACTCTG CAGCCACAGA CATTGCATCC
    TCGCTGTCAG ATGACCAGGT GCCACAGGCC TTCCTGTTCA TGCTGCTGGT CCAGTTTGGC
    ACCATGGTCA TCGACCGTGC CCTCTACCTG CGCAAGACTG TCCTGGGAAA GCTGGCCTTT
    CAGGTGGTCC TGGTGGTGGC GATTCACATC TGGATGTTCT TTATCTTACC GGCTGTCACT
    GAGAGGATGT TCAGCCAGAA TGCGGTGGCA CAGCTGTGGT ACTTCGTCAA GTGCATTTAC
    TTTGCCCTGT CCGCCTACCA GATCCGCTGT GGCTACCCCA CCCGTATCTT GGGCAACTTC
    CTCACCAAGA AATACAACCA TCTAAACCTC TTCCTTTTCC AGGGGTTCCG TCTAGTGCCG
    TTCCTGGTGG AGCTGCGGGC CGTCATGGAC TGGGTGTGGA CCGACACCAC GCTGTCCCTG
    TCCAACTGGA TGTGTGTGGA AGACATCTAT GCCAACATCT TTATCATCAA GTGCAGCCGA
    GAGACAGAGA AGAAATACCC CCAGCCCAAG GGGCAGAAGA AGAAGAAAAT TGTCAAGTAT
    GGTATGGGAG GCCTCATTAT CCTCTTCCTC ATCGCCATCA TCTGGTTCCC TCTGCTCTTC
    ATGTCACTGA TCCGCTCTGT GGTCGGGGTC GTCAACCAGC CCATTGATGT CACCGTCACC
    CTCAAGCTAG GCGGCTATGA GCCACTGTTC ACCATGAGCG CCCAGCAGCC ATCCATTGTG
    CCATTCACAC CCCAGGCCTA CGAGGAGCTG TCCCAGCAGT TTGACCCCTA TCCACTAGCC
    ATGCAGTTCA TTAGCCAGTA CAGTCCTGAG GACATCGTCA CTGCACAGAT CGAGGGCAGC
    TCGGGGGCGC TGTGGCGCAT CAGCCCACCC AGCCGAGCCC AGATGAAGCA GGAGCTGTAC
    AACGGCACAG CCGACATTAC ACTGCGCTTT ACCTGGAATT TCCAAAGGGA CCTGGCCAAG
    GGTGGCACTG TGGAGTATAC TAATGAGAAG CACACCTTGG AGCTGGCCCC CAACAGTACG
    GCACGAAGGC AGCTGGCCCA ACTGCTCGAG GGCAGACCTG ACCAGTCAGT GGTCATTCCC
    CATCTCTTCC CCAAGTACAT TCGTGCTCCC AATGGGCCTG AAGCCAACCC TGTGAAGCAG
    CTGCAGCCAG ATGAGGAAGA GGACTACCTT GGTGTGCGCA TCCAGCTGCG GAGGGAGCAA
    GTGGGCACAG GGGCCTCTGG GGAGCAAGCG GGCACCAAGG CCTCCGACTT CCTCGAGTGG
    TGGGTCATCG AGCTGCAGGA CTGCAAGGCT GACTGCAACC TGCTGCCCAT GGTCATCTTC
    AGTGACAAGG TCAGCCCACC TAGCCTGGGC TTCCTGGCCG GCTACGGGAT TGTGGGGCTG
    TACGTCTCCA TCGTGCTGGT GGTTGGCAAG TTTGTGCGGG GCTTCTTCAG CGAGATCTCT
    CACTCCATCA TGTTCGAGGA ACTGCCGTGT GTGGACCGCA TCCTCAAGCT GTGCCAGGAC
    ATCTTCTTGG TGCGCGAGAC CCGGGAGCTG GAGCTGGAGG AGGAGCTATA CGCCAAGCTC
    ATCTTCCTGT ACCGATCTCC AGAGACCATG ATTAAGTGGA CACGTGAGAG GGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu73792
    Clone ID Related Accession (Same CDS sequence) XM_006530957.3
    Accession Version XM_006530957.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product piezo-type mechanosensitive ion channel component 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530957.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGGTATTC TGTCTGTGGT GCACAAGCCT TATGTATGCA TGCTTACAGC CTCCCTGTTA 
    CGCTTCAATG CTCTCTCGCT GGTCTATTTG CTGTTTCTAC TGCTGCTGCC CTGGCTTCCA
    GGCCCCTCAA GACACAGCAT ACCAGGTCAC ACAGGTCGCC TGCTCCGTGC ACTACTCTGC
    CTCAGCCTCC TCTTCCTGGT GGCCCACCTT GCCTTTCAGA TATGCCTACA CACCGTGCCT
    CACCTGGACC AGTTTCTGGG ACAAAACGGT AGCCTTTGGG TGAAGGTGTC TCAACACATA
    GGGGTTACGA GGCTGGACCT GAAGGACATC TTTAACACCA CCAGGCTGGT AGCACCTGAC
    CTGGGAGTGC TGCTGGCGTC CTCCCTTTGC CTTGGCCTCT GTGGACGCCT CACGAGGAAA
    GCCCGGCAGA GTCGGCGCAC CCAGGAGCTG CAGGATGATG ACGATGACGA TGATGACGAC
    GATGAAGACA TAGATGCTGC CCCAGCCGTG GGGCTGAAGG GAGCCCCTGC CCTGGCAACC
    AAACGCAGGC TGTGGCTGGC CTCCCGCTTC CGGGTCACGG CCCACTGGCT GCTGATGACC
    TCTGGACGGA CGCTGGTCAT TGTGTTGCTG GCCCTGGCAG GCATAGCCCA CCCTTCGGCC
    TTCTCCAGCG TCTACCTGGT GGTGTTCCTG GCCATCTGCA CCTGGTGGTC CTGTCACTTT
    CCTCTCAGCC CCCTGGGCTT TAACACCCTC TGTGTCATGG TGAGCTGTTT TGGTGCCGGC
    CATCTCATTT GCCTATACTG CTATCAGACA CCATTTATCC AGGACATGTT ACCCCCTGGG
    AACATCTGGG CCAGGCTATT TGGTCTCAAG AACTTCGTAG ACCTCCCTAA CTACTCCAGC
    CCCAACGCCC TGGTACTCAA CACTAAGCAC GCCTGGCCCA TCTATGTGAG TCCCGGAATC
    CTGCTGCTGC TATATTACAC AGCCACCTCT CTCCTGAAGC TCCACAAGAG CTGTCCCTCA
    GAGCTGAGGA AGGAGACACC CAGGGAGGAT GAGGAGCATG AGCTGGAACT GGACCACCTG
    GAGCCAGAGC CCCAGGCTAG GGACGCCACC CAGGGTGAGA TGCCCATGAC CACGGAACCT
    GACCTTGACA ACTGCACCGT GCATGTACTA ACCAGCCAGA GCCCTGTCCG CCAGCGTCCA
    GTTCGCCCCA GGCTGGCTGA GCTGAAAGAG ATGTCACCGC TACATGGCCT GGGCCACCTC
    ATCATGGACC AGAGCTATGT GTGTGCCCTG ATTGCCATGA TGGTGTGGAG CATCATGTAC
    CACAGCTGGC TGACCTTCGT CCTGCTGCTC TGGGCCTGCC TCATCTGGAC GGTGCGTAGC
    CGCCACCAGC TGGCTATGCT CTGCTCGCCC TGCATCCTGC TGTATGGGCT GACGCTCTGC
    TGCCTGAGGT ACGTGTGGGC CATGGAACTT CCTGAGCTGC CCACCACCCT GGGCCCTGTC
    AGCCTGCACC AGTTGGGACT GGAACACACA CGCTACCCTT GCCTGGACCT CGGTGCCATG
    CTCCTCTATC TGCTCACATT CTGGCTCCTT CTGCGTCAGT TCGTGAAGGA GAAGCTGCTG
    AAGAAGCAAA AGGTGCCTGC GGCGCTTTTG GAGGTCACGG TGGCCGACAC TGAGCCCACA
    CAGACCCAGA CGCTGCTGCG GAGCCTGGGG GAGCTGGTCA CCGGCATCTA CGTCAAATAC
    TGGATCTATG TGTGCGCCGG CATGTTCATT GTGGTCAGCT TCGCCGGCCG CCTGGTGGTC
    TACAAAATCG TCTACATGTT CCTCTTCCTG CTGTGCCTCA CCTTGTTCCA GGTCTACTAC
    ACCCTGTGGA GGAAGCTGCT GCGTGTCTTC TGGTGGCTCG TGGTGGCCTA TACAATGCTC
    GTGCTCATCG CTGTGTACAC CTTCCAGTTC CAGGACTTCC CCACCTATTG GCGCAACCTC
    ACGGGCTTCA CGGACGAGCA GTTGGGCGAC CTGGGCCTGG AGCAGTTCAG TGTGTCGGAG
    CTCTTTTCCA GTATCCTCAT CCCTGGCTTC TTCCTGCTGG CCTGCATCCT GCAGCTGCAC
    TACTTCCACA GACCGTTCAT GCAGCTCACT GACCTGGAGC ACGTGCCGCC ACCAGGCACC
    CGCCACCCTC GATGGGCTCA CAGGCAGGAT GCAGTGAGCG AGGCCCCTCT GCTTGAGCAT
    CAGGAGGAAG AGGAAGTCTT CAGGGAAGAT GGGCAGAGCA TGGATGGGCC CCACCAGGCC
    ACACAGGTCC CTGAGGGTAC GGCCAGCAAG TGGGGCCTGG TGGCTGACCG GCTGCTGGAC
    CTAGCGGCCA GCTTCTCGGC TGTCCTCACC CGAATCCAGG TGTTCGTTCG GCGCTTGCTA
    GAACTTCACG TCTTCAAGCT GGTGGCCCTC TACACTGTCT GGGTGGCCCT GAAAGAAGTG
    TCTGTGATGA ACCTGCTGCT GGTGGTGCTA TGGGCCTTCG CCTTGCCCTA TCCGCGCTTC
    CGGCCCATGG CTTCCTGCCT GTCCACCGTG TGGACCTGTA TCATCATTGT ATGCAAGATG
    CTCTATCAGC TCAAGATTGT CAACCCGCAT GAGTACTCCA GCAACTGCAC TGAGCCCTTC
    CCCAACAATA CCAACTTGCA GCCTTTGGAG ATCAACCAGT CTTTGCTGTA CCGTGGCCCT
    GTTGACCCTG CCAACTGGTT TGGGGTGCGG AAGGGTTACC CCAACTTGGG CTATATCCAG
    AACCACCTGC AGATCCTTCT GTTGCTGGTG TTTGAGGCCG TGGTGTACCG GCGCCAAGAG
    CACTACCGCC GGCAGCACCA GCAGGCCCCT CTGCCCGCCC AGGCTGTGTG CGCAGATGGC
    ACCCGCCAGA GGTTGGACCA GGACCTACTT AGCTGCCTCA AGTATTTCAT CAACTTCTTC
    TTCTACAAAT TCGGGCTGGA GATCTGCTTC TTGATGGCCG TGAATGTGAT TGGGCAGCGT
    ATGAACTTCA TGGTGATCTT ACACGGTTGC TGGTTGGTGG CCATCCTTAC ACGCCGGCGC
    CGTGAGGCCA TCGCCCGCCT CTGGCCTAAC TACTGTCTGT TCCTCACGCT GTTCCTGCTG
    TACCAGTACC TGCTGTGTTT GGGCATGCCC CCTGCACTGT GCATTGACTA TCCATGGCGC
    TGGAGCAAGG CCATCCCCAT GAATTCCGCC CTCATCAAGT GGCTGTACCT ACCTGACTTC
    TTCAGAGCCC CCAACTCCAC CAACCTTATC AGTGACTTCC TCCTGCTGCT TTGCGCCTCC
    CAGCAGTGGC AGGTCTTCTC AGCGGAGCGA ACGGAGGAGT GGCAACGCAT GGCGGGCATC
    AACACTGACC ACCTGGAGCC CCTGCGTGGG GAGCCCAACC CTATACCCAA CTTCATCCAC
    TGCAGGTCCT ATCTGGATAT GCTGAAGGTG GCCGTCTTCC GCTACCTGTT CTGGCTGGTG
    CTCGTTGTGG TGTTTGTTGC GGGGGCCACC CGCATAAGCA TCTTCGGGCT GGGGTACCTG
    CTAGCCTGCT TCTACCTGCT GCTGTTTGGC ACTACCCTGC TGCAGAAGGA CACGCGAGCC
    CAGCTCGTGC TGTGGGACTG CCTCATCCTC TATAATGTCA CTGTCATCAT CTCTAAGAAT
    ATGCTGTCGC TCCTGTCCTG TGTCTTCGTG GAGCAAATGC AGAGCAACTT CTGCTGGGTC
    ATCCAGCTCT TCAGCCTCGT GTGCACAGTC AAAGGCTACT ATGATCCCAA AGAGATGATG
    ACCAGGGACC GGGACTGCCT GCTGCCTGTG GAGGAGGCCG GGATCATCTG GGACAGTATC
    TGCTTCTTCT TCCTGCTCTT GCAACGGCGC ATCTTTCTCA GCCACTACTT CCTGCATGTC
    AGCGCTGACC TGAAAGCCAC AGCCCTGCAG GCATCCAGGG GCTTTGCCCT CTACAATGCA
    GCCAACCTGA AGAGCATCAA CTTCCATCGC CAGATTGAGG AGAAGTCCCT GGCCCAGCTG
    AAAAGACAGA TGAAGCGCAT CCGTGCCAAA CAGGAGAAGT ACAGGCAGAG CCAGGCAAGT
    CGTGGCCAAC TCCAGTCCAA AGACCCTCAG GATCCCAGCC AGGAGCCAGG GCCTGACAGC
    CCAGGGGGCT CCTCCCCGCC ACGGAGACAG TGGTGGCGCC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGCGACTA CTTCCTGTTT GAGTCAGATA GCGAGGAGGA AGAGGAGGCC
    CTACCTGAGG ACCCCAGGCC TGCAGCTCAG AGTGCCTTCC AGATGGCATA CCAGGCATGG
    GTAACCAATG CCCAGACAGT GCTGAGGCAG CGTCGGGAGC GGGCACGGCA GGAGCGGGCA
    GAGCAGCTGG CTTCTGGAGG TGACTTGAAC CCAGATGTGG AACCAGTAGA TGTCCCAGAA
    GATGAGATGG CAGGCCGTAG CCACATGATG CAGCGTGTGC TAAGCACCAT GCAGTTCCTG
    TGGGTGCTGG GCCAGGCCAC GGTAGACGGG CTGACGCGCT GGCTGCGTGC ATTCACGAAG
    CACCACCGCA CCATGAGCGA TGTGCTGTGC GCAGAGCGCT ACCTGCTCAC CCAGGAGCTT
    CTTCGGGTTG GAGAGGTACG CCGAGGTGTG CTGGACCAGC TTTATGTGGG TGAAGATGAG
    GCCACATTGT CAGGTCCCAT GGAGACCCGG GATGGACCCA GCACAGCCTC AAGTGGGCTG
    GGAGCCGAAG AGCCTTTGAG TAGCATGACA GACGACACCA GCAGCCCCCT GAGCACAGGC
    TATAACACCC GCAGTGGCAG TGAGGAGATT GTCACCGACG CTGGGGACCT CCAGGCTGGG
    ACCTCCCTGC ACGGCTCCCA AGAGCTTTTA GCCAATGCTC GTACCCGGAT GCGCACGGCC
    AGCGAGCTGC TACTGGATAG GCGCCTGCAT ATCCCTGAGC TGGAGGAGGC CGAGCGGTTT
    GAGGCACAGC AGGGCCGGAC TCTGCGGCTG CTCAGGGCTG GGTACCAGTG CGTGGCGGCA
    CACTCGGAGC TGCTCTGTTA CTTCATCATC ATCCTTAACC ACATGGTGAC AGCCTCGGCT
    GCCTCCCTGG TGCTGCCCGT GCTTGTGTTC CTGTGGGCCA TGCTGACCAT CCCGAGGCCT
    AGCAAGCGCT TTTGGATGAC AGCTATCGTC TTCACTGAGG TCATGGTGGT CACCAAATAC
    CTGTTCCAGT TCGGCTTCTT CCCCTGGAAC AGCTACGTTG TGCTGCGGCG CTATGAGAAC
    AAGCCCTACT TCCCTCCGCG AATCCTGGGC CTTGAGAAAA CGGACAGCTA CATCAAGTAT
    GACCTGGTGC AGCTCATGGC CCTCTTCTTC CACCGCTCGC AGCTACTGTG TTATGGCCTC
    TGGGACCATG AGGAGGATCG CTATCCCAAG GACCATTGCA GGAGTAGTGT GAAGGACCGG
    GAGGCCAAGG AAGAGCCAGA AGCTAAGCTG GAATCGCAGT CTGAGACAGG CACTGGGCAT
    CCCAAGGAGC CAGTGTTGGC CGGTACTCCC AGGGACCACA TCCAAGGGAA AGGAAGTATT
    AGATCCAAGG ATGTTATCCA AGATCCCCCA GAGGACCTTA AGCCCCGGCA CACGAGGCAC
    ATCAGCATAC GCTTCAGGAG GCGCAAGGAG ACTCCAGGAC CCAAAGGAAC AGCAGTCATG
    GAGACTGAGC ACGAGGAGGG AGAAGGAAAA GAAACTACAG AGAGAAAGAG GCCGCGTCAC
    ACTCAAGAAA AATCGAAGTT TCGGGAGAGA ATGAAGGCAG CTGGGCGCCG GCTGCAGAGC
    TTCTGTGTGT CACTGGCCCA GAGCTTCTAC CAACCCTTGC AGCGTTTCTT CCATGACATT
    CTGCACACAA AGTACCGGGC GGCCACCGAC GTCTACGCCC TCATGTTCCT GGCCGATATT
    GTCGACATCA TCATCATCAT CTTTGGTTTT TGGGCTTTTG GGAAGCACTC TGCAGCCACA
    GACATTGCAT CCTCGCTGTC AGATGACCAG GTGCCACAGG CCTTCCTGTT CATGCTGCTG
    GTCCAGTTTG GCACCATGGT CATCGACCGT GCCCTCTACC TGCGCAAGAC TGTCCTGGGA
    AAGCTGGCCT TTCAGGTGGT CCTGGTGGTG GCGATTCACA TCTGGATGTT CTTTATCTTA
    CCGGCTGTCA CTGAGAGGAT GTTCAGCCAG AATGCGGTGG CACAGCTGTG GTACTTCGTC
    AAGTGCATTT ACTTTGCCCT GTCCGCCTAC CAGATCCGCT GTGGCTACCC CACCCGTATC
    TTGGGCAACT TCCTCACCAA GAAATACAAC CATCTAAACC TCTTCCTTTT CCAGGGGTTC
    CGTCTAGTGC CGTTCCTGGT GGAGCTGCGG GCCGTCATGG ACTGGGTGTG GACCGACACC
    ACGCTGTCCC TGTCCAACTG GATGTGTGTG GAAGACATCT ATGCCAACAT CTTTATCATC
    AAGTGCAGCC GAGAGACAGA GAAGGCTTCC TCGCCTCAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006531020.2
    CDS16..6540
    Translation

    Target ORF information:

    RefSeq Version XM_006530957.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530957.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGGTATTC TGTCTGTGGT GCACAAGCCT TATGTATGCA TGCTTACAGC CTCCCTGTTA 
    CGCTTCAATG CTCTCTCGCT GGTCTATTTG CTGTTTCTAC TGCTGCTGCC CTGGCTTCCA
    GGCCCCTCAA GACACAGCAT ACCAGGTCAC ACAGGTCGCC TGCTCCGTGC ACTACTCTGC
    CTCAGCCTCC TCTTCCTGGT GGCCCACCTT GCCTTTCAGA TATGCCTACA CACCGTGCCT
    CACCTGGACC AGTTTCTGGG ACAAAACGGT AGCCTTTGGG TGAAGGTGTC TCAACACATA
    GGGGTTACGA GGCTGGACCT GAAGGACATC TTTAACACCA CCAGGCTGGT AGCACCTGAC
    CTGGGAGTGC TGCTGGCGTC CTCCCTTTGC CTTGGCCTCT GTGGACGCCT CACGAGGAAA
    GCCCGGCAGA GTCGGCGCAC CCAGGAGCTG CAGGATGATG ACGATGACGA TGATGACGAC
    GATGAAGACA TAGATGCTGC CCCAGCCGTG GGGCTGAAGG GAGCCCCTGC CCTGGCAACC
    AAACGCAGGC TGTGGCTGGC CTCCCGCTTC CGGGTCACGG CCCACTGGCT GCTGATGACC
    TCTGGACGGA CGCTGGTCAT TGTGTTGCTG GCCCTGGCAG GCATAGCCCA CCCTTCGGCC
    TTCTCCAGCG TCTACCTGGT GGTGTTCCTG GCCATCTGCA CCTGGTGGTC CTGTCACTTT
    CCTCTCAGCC CCCTGGGCTT TAACACCCTC TGTGTCATGG TGAGCTGTTT TGGTGCCGGC
    CATCTCATTT GCCTATACTG CTATCAGACA CCATTTATCC AGGACATGTT ACCCCCTGGG
    AACATCTGGG CCAGGCTATT TGGTCTCAAG AACTTCGTAG ACCTCCCTAA CTACTCCAGC
    CCCAACGCCC TGGTACTCAA CACTAAGCAC GCCTGGCCCA TCTATGTGAG TCCCGGAATC
    CTGCTGCTGC TATATTACAC AGCCACCTCT CTCCTGAAGC TCCACAAGAG CTGTCCCTCA
    GAGCTGAGGA AGGAGACACC CAGGGAGGAT GAGGAGCATG AGCTGGAACT GGACCACCTG
    GAGCCAGAGC CCCAGGCTAG GGACGCCACC CAGGGTGAGA TGCCCATGAC CACGGAACCT
    GACCTTGACA ACTGCACCGT GCATGTACTA ACCAGCCAGA GCCCTGTCCG CCAGCGTCCA
    GTTCGCCCCA GGCTGGCTGA GCTGAAAGAG ATGTCACCGC TACATGGCCT GGGCCACCTC
    ATCATGGACC AGAGCTATGT GTGTGCCCTG ATTGCCATGA TGGTGTGGAG CATCATGTAC
    CACAGCTGGC TGACCTTCGT CCTGCTGCTC TGGGCCTGCC TCATCTGGAC GGTGCGTAGC
    CGCCACCAGC TGGCTATGCT CTGCTCGCCC TGCATCCTGC TGTATGGGCT GACGCTCTGC
    TGCCTGAGGT ACGTGTGGGC CATGGAACTT CCTGAGCTGC CCACCACCCT GGGCCCTGTC
    AGCCTGCACC AGTTGGGACT GGAACACACA CGCTACCCTT GCCTGGACCT CGGTGCCATG
    CTCCTCTATC TGCTCACATT CTGGCTCCTT CTGCGTCAGT TCGTGAAGGA GAAGCTGCTG
    AAGAAGCAAA AGGTGCCTGC GGCGCTTTTG GAGGTCACGG TGGCCGACAC TGAGCCCACA
    CAGACCCAGA CGCTGCTGCG GAGCCTGGGG GAGCTGGTCA CCGGCATCTA CGTCAAATAC
    TGGATCTATG TGTGCGCCGG CATGTTCATT GTGGTCAGCT TCGCCGGCCG CCTGGTGGTC
    TACAAAATCG TCTACATGTT CCTCTTCCTG CTGTGCCTCA CCTTGTTCCA GGTCTACTAC
    ACCCTGTGGA GGAAGCTGCT GCGTGTCTTC TGGTGGCTCG TGGTGGCCTA TACAATGCTC
    GTGCTCATCG CTGTGTACAC CTTCCAGTTC CAGGACTTCC CCACCTATTG GCGCAACCTC
    ACGGGCTTCA CGGACGAGCA GTTGGGCGAC CTGGGCCTGG AGCAGTTCAG TGTGTCGGAG
    CTCTTTTCCA GTATCCTCAT CCCTGGCTTC TTCCTGCTGG CCTGCATCCT GCAGCTGCAC
    TACTTCCACA GACCGTTCAT GCAGCTCACT GACCTGGAGC ACGTGCCGCC ACCAGGCACC
    CGCCACCCTC GATGGGCTCA CAGGCAGGAT GCAGTGAGCG AGGCCCCTCT GCTTGAGCAT
    CAGGAGGAAG AGGAAGTCTT CAGGGAAGAT GGGCAGAGCA TGGATGGGCC CCACCAGGCC
    ACACAGGTCC CTGAGGGTAC GGCCAGCAAG TGGGGCCTGG TGGCTGACCG GCTGCTGGAC
    CTAGCGGCCA GCTTCTCGGC TGTCCTCACC CGAATCCAGG TGTTCGTTCG GCGCTTGCTA
    GAACTTCACG TCTTCAAGCT GGTGGCCCTC TACACTGTCT GGGTGGCCCT GAAAGAAGTG
    TCTGTGATGA ACCTGCTGCT GGTGGTGCTA TGGGCCTTCG CCTTGCCCTA TCCGCGCTTC
    CGGCCCATGG CTTCCTGCCT GTCCACCGTG TGGACCTGTA TCATCATTGT ATGCAAGATG
    CTCTATCAGC TCAAGATTGT CAACCCGCAT GAGTACTCCA GCAACTGCAC TGAGCCCTTC
    CCCAACAATA CCAACTTGCA GCCTTTGGAG ATCAACCAGT CTTTGCTGTA CCGTGGCCCT
    GTTGACCCTG CCAACTGGTT TGGGGTGCGG AAGGGTTACC CCAACTTGGG CTATATCCAG
    AACCACCTGC AGATCCTTCT GTTGCTGGTG TTTGAGGCCG TGGTGTACCG GCGCCAAGAG
    CACTACCGCC GGCAGCACCA GCAGGCCCCT CTGCCCGCCC AGGCTGTGTG CGCAGATGGC
    ACCCGCCAGA GGTTGGACCA GGACCTACTT AGCTGCCTCA AGTATTTCAT CAACTTCTTC
    TTCTACAAAT TCGGGCTGGA GATCTGCTTC TTGATGGCCG TGAATGTGAT TGGGCAGCGT
    ATGAACTTCA TGGTGATCTT ACACGGTTGC TGGTTGGTGG CCATCCTTAC ACGCCGGCGC
    CGTGAGGCCA TCGCCCGCCT CTGGCCTAAC TACTGTCTGT TCCTCACGCT GTTCCTGCTG
    TACCAGTACC TGCTGTGTTT GGGCATGCCC CCTGCACTGT GCATTGACTA TCCATGGCGC
    TGGAGCAAGG CCATCCCCAT GAATTCCGCC CTCATCAAGT GGCTGTACCT ACCTGACTTC
    TTCAGAGCCC CCAACTCCAC CAACCTTATC AGTGACTTCC TCCTGCTGCT TTGCGCCTCC
    CAGCAGTGGC AGGTCTTCTC AGCGGAGCGA ACGGAGGAGT GGCAACGCAT GGCGGGCATC
    AACACTGACC ACCTGGAGCC CCTGCGTGGG GAGCCCAACC CTATACCCAA CTTCATCCAC
    TGCAGGTCCT ATCTGGATAT GCTGAAGGTG GCCGTCTTCC GCTACCTGTT CTGGCTGGTG
    CTCGTTGTGG TGTTTGTTGC GGGGGCCACC CGCATAAGCA TCTTCGGGCT GGGGTACCTG
    CTAGCCTGCT TCTACCTGCT GCTGTTTGGC ACTACCCTGC TGCAGAAGGA CACGCGAGCC
    CAGCTCGTGC TGTGGGACTG CCTCATCCTC TATAATGTCA CTGTCATCAT CTCTAAGAAT
    ATGCTGTCGC TCCTGTCCTG TGTCTTCGTG GAGCAAATGC AGAGCAACTT CTGCTGGGTC
    ATCCAGCTCT TCAGCCTCGT GTGCACAGTC AAAGGCTACT ATGATCCCAA AGAGATGATG
    ACCAGGGACC GGGACTGCCT GCTGCCTGTG GAGGAGGCCG GGATCATCTG GGACAGTATC
    TGCTTCTTCT TCCTGCTCTT GCAACGGCGC ATCTTTCTCA GCCACTACTT CCTGCATGTC
    AGCGCTGACC TGAAAGCCAC AGCCCTGCAG GCATCCAGGG GCTTTGCCCT CTACAATGCA
    GCCAACCTGA AGAGCATCAA CTTCCATCGC CAGATTGAGG AGAAGTCCCT GGCCCAGCTG
    AAAAGACAGA TGAAGCGCAT CCGTGCCAAA CAGGAGAAGT ACAGGCAGAG CCAGGCAAGT
    CGTGGCCAAC TCCAGTCCAA AGACCCTCAG GATCCCAGCC AGGAGCCAGG GCCTGACAGC
    CCAGGGGGCT CCTCCCCGCC ACGGAGACAG TGGTGGCGCC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGCGACTA CTTCCTGTTT GAGTCAGATA GCGAGGAGGA AGAGGAGGCC
    CTACCTGAGG ACCCCAGGCC TGCAGCTCAG AGTGCCTTCC AGATGGCATA CCAGGCATGG
    GTAACCAATG CCCAGACAGT GCTGAGGCAG CGTCGGGAGC GGGCACGGCA GGAGCGGGCA
    GAGCAGCTGG CTTCTGGAGG TGACTTGAAC CCAGATGTGG AACCAGTAGA TGTCCCAGAA
    GATGAGATGG CAGGCCGTAG CCACATGATG CAGCGTGTGC TAAGCACCAT GCAGTTCCTG
    TGGGTGCTGG GCCAGGCCAC GGTAGACGGG CTGACGCGCT GGCTGCGTGC ATTCACGAAG
    CACCACCGCA CCATGAGCGA TGTGCTGTGC GCAGAGCGCT ACCTGCTCAC CCAGGAGCTT
    CTTCGGGTTG GAGAGGTACG CCGAGGTGTG CTGGACCAGC TTTATGTGGG TGAAGATGAG
    GCCACATTGT CAGGTCCCAT GGAGACCCGG GATGGACCCA GCACAGCCTC AAGTGGGCTG
    GGAGCCGAAG AGCCTTTGAG TAGCATGACA GACGACACCA GCAGCCCCCT GAGCACAGGC
    TATAACACCC GCAGTGGCAG TGAGGAGATT GTCACCGACG CTGGGGACCT CCAGGCTGGG
    ACCTCCCTGC ACGGCTCCCA AGAGCTTTTA GCCAATGCTC GTACCCGGAT GCGCACGGCC
    AGCGAGCTGC TACTGGATAG GCGCCTGCAT ATCCCTGAGC TGGAGGAGGC CGAGCGGTTT
    GAGGCACAGC AGGGCCGGAC TCTGCGGCTG CTCAGGGCTG GGTACCAGTG CGTGGCGGCA
    CACTCGGAGC TGCTCTGTTA CTTCATCATC ATCCTTAACC ACATGGTGAC AGCCTCGGCT
    GCCTCCCTGG TGCTGCCCGT GCTTGTGTTC CTGTGGGCCA TGCTGACCAT CCCGAGGCCT
    AGCAAGCGCT TTTGGATGAC AGCTATCGTC TTCACTGAGG TCATGGTGGT CACCAAATAC
    CTGTTCCAGT TCGGCTTCTT CCCCTGGAAC AGCTACGTTG TGCTGCGGCG CTATGAGAAC
    AAGCCCTACT TCCCTCCGCG AATCCTGGGC CTTGAGAAAA CGGACAGCTA CATCAAGTAT
    GACCTGGTGC AGCTCATGGC CCTCTTCTTC CACCGCTCGC AGCTACTGTG TTATGGCCTC
    TGGGACCATG AGGAGGATCG CTATCCCAAG GACCATTGCA GGAGTAGTGT GAAGGACCGG
    GAGGCCAAGG AAGAGCCAGA AGCTAAGCTG GAATCGCAGT CTGAGACAGG CACTGGGCAT
    CCCAAGGAGC CAGTGTTGGC CGGTACTCCC AGGGACCACA TCCAAGGGAA AGGAAGTATT
    AGATCCAAGG ATGTTATCCA AGATCCCCCA GAGGACCTTA AGCCCCGGCA CACGAGGCAC
    ATCAGCATAC GCTTCAGGAG GCGCAAGGAG ACTCCAGGAC CCAAAGGAAC AGCAGTCATG
    GAGACTGAGC ACGAGGAGGG AGAAGGAAAA GAAACTACAG AGAGAAAGAG GCCGCGTCAC
    ACTCAAGAAA AATCGAAGTT TCGGGAGAGA ATGAAGGCAG CTGGGCGCCG GCTGCAGAGC
    TTCTGTGTGT CACTGGCCCA GAGCTTCTAC CAACCCTTGC AGCGTTTCTT CCATGACATT
    CTGCACACAA AGTACCGGGC GGCCACCGAC GTCTACGCCC TCATGTTCCT GGCCGATATT
    GTCGACATCA TCATCATCAT CTTTGGTTTT TGGGCTTTTG GGAAGCACTC TGCAGCCACA
    GACATTGCAT CCTCGCTGTC AGATGACCAG GTGCCACAGG CCTTCCTGTT CATGCTGCTG
    GTCCAGTTTG GCACCATGGT CATCGACCGT GCCCTCTACC TGCGCAAGAC TGTCCTGGGA
    AAGCTGGCCT TTCAGGTGGT CCTGGTGGTG GCGATTCACA TCTGGATGTT CTTTATCTTA
    CCGGCTGTCA CTGAGAGGAT GTTCAGCCAG AATGCGGTGG CACAGCTGTG GTACTTCGTC
    AAGTGCATTT ACTTTGCCCT GTCCGCCTAC CAGATCCGCT GTGGCTACCC CACCCGTATC
    TTGGGCAACT TCCTCACCAA GAAATACAAC CATCTAAACC TCTTCCTTTT CCAGGGGTTC
    CGTCTAGTGC CGTTCCTGGT GGAGCTGCGG GCCGTCATGG ACTGGGTGTG GACCGACACC
    ACGCTGTCCC TGTCCAACTG GATGTGTGTG GAAGACATCT ATGCCAACAT CTTTATCATC
    AAGTGCAGCC GAGAGACAGA GAAGGCTTCC TCGCCTCAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.