Piezo1 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Piezo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Piezo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa39814 NM_001077200.2
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa47912 XM_008772626.2
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa47913 XM_008772627.2
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa47914 XM_017601325.1
Latest version!
Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa39814
    Clone ID Related Accession (Same CDS sequence) NM_001077200.2
    Accession Version NM_001077200.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7608bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC134009.3, AB161229.1 and BQ196181.1. On Sep 3, 2009 this sequence version replaced NM_001077200.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG
    CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC
    CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGTAACCT TTGGGCAAAC
    GTGTCTCAAC ACATAGGGGT TACAAGGCTG GACCTGAAGG ACATCTTTAA CACCACCAGA
    CTGGTGGCAC CTGACCTGGG AGTGCTGGTG GCATCTTCCC TTTGCCTTGG CCTCTGTGGA
    CGCCTCACAA GGAAAGCCCG GCAGAGCCAG CGCACTCAGG AGCTGGAGGA GGATGACATA
    GATGCTGCCC CCGCCGCAGG GCTGCAGGGA GCCCCCACCC TGGCAACCAA ACGCAGGCTG
    TGGCTGGCCC CTCGCTTCCG GATCACGGCG CACTGGCTGC TGGTGACCTC CGGACGGATG
    CTGGTCATCG TGCTACTGGC ACTCGCAGGC ATAGCCCATC CTTCGGCCTT CTCCAGCGTC
    TACCTGATGG TGTTCCTGGC CATCTGCACC TGGTGGTCTT GCCACTTTCC TCTCAGCTCC
    CTGGGCTTTA ACACCCTCTG CGTCATGGTG AGCTGCTTTG GCGCCGGCCA TCTTGTTTGT
    TTATACTGCT ATCAGACACC ATTTGTCCAG AGCGTGCTAC TTCCTGGCAG CCTCTGGGCC
    AGGCTATTTG GTCTCAAGAA CTTCGTAGAC ATCCCTAACT GCTCCAGCCC CAACGTGCTG
    GTACTCAACA CTAAGCATGC TTGGCCCATC TACGTGAGCC CCGGTATCCT GCTGCTGTTG
    TACTACACAG CCACCTCTCT CCTGAAGCTC CGCAAGGGCC GCTTCTCAGA GCTGAGGAAG
    GAGATCCCGA GGGAGGATGA GGAGCATGAA CTGGAACTGG ACCAGCTGGA GCCAGAGCCC
    CAGGCTAGGG GCACCACCCA GGGTGCGACG CCCACGACCA CTGGACCCGA CATTGACAAC
    TGCACCGTGC ATGTACTAAC CAGCCAGAGC CCCGTCCGCC AGCGTCCTGT ACGCCCCAGG
    CTGGCTGAGC TGAAAGAGAT GTCACCACTA CATGGCCTGG GCCACCTCAT CCTGGACCAG
    AGCTATGTGT GTGCTCTGAT CGCCATGATG GTGTGGAGCA TCATGTACCA CAGCTGGCTG
    ACCTTCGTCT TGCTGCTCTG GGCCTGCCTC ATCTGGACCG TGCGCAGCCG CCACCAGTTG
    GCTATGCTGT GCTCACCCTG CATCCTGCTG TACGGACTGA CACTCTGCTG CCTGCGGTAC
    GTGTGGGCCA TGGAGCTCCC CGAGCTGCCC ACCACCCTGG GCCCTGTCAG TCTACATCAG
    TTGGGATTGG AGCATACACG CTACCCCTGC CTGGACCTTG GTGCCATGCT GCTGTATCTG
    CTCACGTTCT GGCTGCTCCT GCGTCAGTTC GTGAAGGAGA AGCTGCTGAA GAAGAGAAAG
    GCACCTTCGA CGCTGCTGGA GGTCACAGTG TCCGACACAG AGCCCACACA GACCCAGACA
    CTGCTGAGGA GCCTGGGGGA GCTGGTCACG GGCATCTACG TCAAGTACTG GATCTACGTG
    TGCGCTGGGA TGTTCATTGT GGTCAGCTTC GCTGGCCGCC TGGTGGTCTA CAAAATCGTC
    TACATGTTCC TTTTCCTGCT GTGCCTCACG TTGTTCCAGG TCTACTACAC TCTGTGGAGG
    AAGCTGCTCC GAGTTTTCTG GTGGCTCGTG GTGGCCTACA CCATGCTCGT GCTCATCGCC
    GTGTACACTT TCCAGTTCCA GGACTTCCCC ACCTACTGGC GCAACCTCAC GGGCTTCACA
    GATGAACAGT TGGGCGACCT GGGCCTGGAG CAGTTCAGCG TGTCGGAGCT CTTTTCCAGC
    ATCCTCATCC CTGGCTTCTT CCTGCTGGCC TGCATCCTGC AGCTGCACTA CTTCCACAGG
    CCGTTCATGC AGCTCACCGA CCTGGAGCAC GTGCCCCCGC CAGGCACACG CCGCCTTCGC
    TGGGCTCACA GGCAGGATAC AGTGAGCGAG GCCCCTCTGC TTCAGCATCA GGAGGAGGAG
    GAAGTCTTCA GGGATGACGG GCAGAGCATG GATGGGCCCC ACCAGACCAC ACAGGTCCCT
    GAGGGTACAG CCAGCAAGTG GGGCCTGGTG GCCGACCGGC TGCTGGACCT AGCGTCCAGC
    TTCTCCGCCG TCCTCACCCG CATCCAGGTG TTCGTACGAT GTTTGCTCGA GCTTCACGTC
    TTCAAGCTGG TGGCTCTCTA CACTGTCTGG GTGGCCCTGA AAGAAGTGTC CGTGATGAAC
    CTGCTGCTGG TGGTGCTGTG GGCCTTCGCC CTGCCCTACC CGCGCTTCCG GCCCATGGCC
    TCCTGCCTGT CCACCGTGTG GACCTGTATC ATCATCGTGT GCAAGATGCT GTATCAGCTG
    AAGATTGTCA ACCCACACGA GTACTCCAGT AACTGCACCG AGCCCTTTCC CAACAATACC
    AACTTGCAGC CCTTGGAGAT CAGCCAGTCT CTGCTGTACC GTGGCCCCGT TGACCCTGCC
    AACTGGTTTG GGGTGCGGAA GGGTTACCCA AACTTGGGCT ATATCCAGAA CCACCTGCAG
    ATCCTTCTGC TGCTGGTGTT TGAGGCTGTG GTGTACCGGC GCCAAGAGCA CTACCGCCGG
    CAGCACCAGC AGGCCCCTCT GCCCGCCCAG GCTTTGTGCG CTGATGGCAC CCGCCAGAGG
    CTGGACCAGG ACCTACTCAG CTGCCTCAAA TATTTCATCA ACTTCTTCTT CTACAAATTC
    GGGCTGGAGA TCTGCTTCTT GATGGCCGTG AATGTCATCG GACAACGCAT GAACTTCATG
    GTGATCTTAC ACGGCTGCTG GTTGGTGGCC ATCCTTACAC GCCGGCGCCG TGAGGCCATC
    GCCCGCCTCT GGCCCAATTA CTGTCTGTTC CTCACGCTGT TCCTGCTGTA CCAGTACCTG
    CTGTGCCTGG GCATGCCTCC CGCACTGTGC ATCGACTACC CGTGGCGCTG GAGCCAGGCC
    ATCCCCATGA ATTCTGCGCT CATCAAGTGG CTGTACCTCC CTGACTTCTT CAGGGCCCCC
    AACTCCACCA ACCTTATCAG TGACTTCCTC CTGCTACTCT GCGCCTCCCA ACAGTGGCAG
    GTGTTCTCCG CAGAGCAAAC GGAAGAATGG CAGCGCATGG CTGGCGTTAA CACTGACCAC
    CTGGAGCCCC TGCGTGGGGA GCCCAACCCT ATCCCCAACT TCATCCACTG CAGGTCCTAT
    CTGGATATGC TGAAGGTGGC CGTCTTCCGC TACCTGTTCT GGCTGGTGCT GGTGGTCGTG
    TTTGTCACAG GGGCCACCCG CATCAGCATC TTTGGGCTGG GGTACCTGCT GGCCTGCTTC
    TACCTGCTGC TCTTTGGCAC CACCCTGCTG CAGAAGGACA CACGAGCCCA GCTCGTGCTG
    TGGGACTGCC TCATCCTCTA CAACGTCACC GTCATCATCT CTAAGAATAT GTTGTCGCTC
    CTGTCCTGTG TCTTCGTGGA GCAAATGCAG AGCAACTTCT GCTGGGTCAT CCAGCTCTTC
    AGCCTCGTGT GCACAGTCAA AGGCTACTAT GACCCCAAAG AGATGAAGAC CAGGGACCGG
    GATTGCCTGC TGCCTGTGGA GGAGGCCGGG ATCATCTGGG ACAGCATCTG CTTCTTCTTC
    CTGCTCCTAC AACGGCGCGT CTTTCTCAGC CACTACTTCC TGCACGTCAG TGCTGACCTG
    AAGGCCACGG CCCTGCAGGC CTCCAGGGGT TTTGCTCTCT ACAATGCGGC CAACATAAAG
    AACATCAACT TCCACCGTCA GACCGAGGAG AGGTCCCTGG CCCAGCTGAA AAGACAGATG
    AAGCGCATCC GTGCCAAACA GGAGAAATAC CGGCAGAGCC AGGCAAGCCG TGGTCAACTC
    CAGTCCACAG ACCCTCAGGA GCCAGGGCCT GACAGCCCAG GGGGTTCCTC CCCGCCACGG
    ACACAGTGGT GGCGCCCCTG GCAGGACCAC GCCACAGTCA TCCACTCTGG TGACTACTTC
    CTGTTTGAGT CGGACAGTGA GGAGGAAGAG GAGGCCCTGC CTGAGGACCC CAGGCCTGCA
    GCTCAGAGTG CCTTCCAGAT GGCATACCAG GCATGGGTAA CCAACGCCCA GACAGTGCTA
    AGGCAGCGTC GTGAACAGGC ACGGCGGGAT CGGGCAGAAC AGCTGGCTTC TGGAGGCGAC
    TTGAGTCCTG AGGTGGAGCT GGTAGACGTC CCAGAGAATG AGATGGCAGG CCATAGCCAT
    GTGATGCAGC GCGTGCTGAG TACCATGCAG TTCCTGTGGG TGCTGGGCCA GGCCACAGTG
    GACGGGCTGA CGCGCTGGTT GCGTACGTTC ACGAAGGACC ACCGCACCAT GAGTGATGTG
    CTGTGTGCAG AGCGTTACCT GCTCACCCAG GAGCTTCTTC GGGGTGGAGA GGTACACCGA
    GGGGTACTGG ACCAGCTTTA CGTGAGCGAA GATGAGATCG CACTGTCAGG TCCTGTGGAG
    AACCGTGATG GACCCAGCAC AGCCTCTAGC GGGCTGGGAG CTGAAGAGCC TTTGAGTAGC
    ATGACAGACG ACACCGGCAG CCCCCTTAGC ACTGGCTACA ACACTCGCAG TGGTAGTGAG
    GAGATTATCA CCGACACTGG GGGCCTCCAG GCTGGGACCT CCCTGCACGG CTCCCAAGAG
    CTTCTAGCCA ATGCTCGTAC CCGGATGCGC ACAGCCAGTG AGCTGCTGCT GGACAGGCGC
    CTGCGTATCC CTGAGCTGGA GGAGGCCGAG CAGTTTGAGG CACAGCAGGG CCGGACGCTG
    CGGCTGCTCA GAGCCATGTA CCAGTGTGTG GCGGCCCACT CAGAGCTTCT CTGTTACTTC
    ATCATCATCC TTAACCACAT GGTGACAGCC TCGGCTGCCT CCCTGGTGCT GCCTGTGCTT
    GTGTTCCTCT GGGCCATGCT GACCATCCCG AGGCCTAGCA AGCGCTTCTG GATGACAGCT
    ATCGTCTTCA CTGAGGTCAT GGTGGTCACC AAATACTTGT TCCAGTTTGG CTTCTTCCCC
    TGGAACAGCT ACATAGTGCT TCGACGCTAC GAGAACAAGC CCTACTTCCC TCCGCGCATC
    CTGGGCCTCG AGAAAACAGA CAGCTATATC AAGTACGACC TGGTGCAACT CATGGCCCTC
    TTCTTCCACC GCTCGCAGTT ACTGTGCTAT GGCCTCTGGG ACCATGAGGA GGATGGTGTC
    CCCAAGGACC ATTGCAGGAG TAGCGAAAAG GACCAGGAAG CTGAGGAAGA GTCAGAGGCT
    AAGCTGGAAT CGCAGCCTGA GACGGGCACT GGGCATCCTG AGGAGCCCGT GTTGACTGGT
    ACCCCCAAGG ACCACATCCA AGGGAAAGGA AGTGTTAGGT CCAAGGATGA GATCCAAGAT
    CCCCCAGAGG ACCTTAAGCC CCAGCACAGG AGACACATCA GCATACGCTT CAGGAGGAGG
    AAGGAGACTC AAGGACCCAA AGGAGCAGCA GTCGTGGAGG CTGAGCACGA GGAGGGAGAG
    GAAGGAAGAG AAGCTGCAGG GAGAAAGAGG CTGCGTCGCC CTCGAGAAGG GTTGAAGATT
    CGGGAGAAAA TGAAGGCAGC TGGACGCCGG CTGCAGAGCT TCTGTCTGTC ACTGGCCCAG
    AGCTTTTACC AACCCCTGCG GCGTTTCTTC CATGACATTC TGCACACCAA GTACCGAGCG
    GCCACCGACG TCTATGCCCT CATGTTCCTG GCTGACATTG TCGACATTGT CGTCATCATC
    TTCGGTTTTT GGGCTTTTGG GAAGCACTCC GCGGCCACGG ACATTGCATC CTCACTGTCA
    GATGACCAGG TGCCACAGGC CTTCCTGTTC ATGCTGCTGG TCCAGTTCGG CACCATGGTC
    ATAGACCGCG CCCTCTACCT GCGTAAGACT GTACTGGGCA ACCTGGCCTT TCAGGTGGTC
    CTGGTGGTGG CCATTCACCT CTGGATGTTT TTCATCTTAC CGGCTGTCAC CGAGAGGATG
    TTCAGACAGA ACGCGGTGGC CCAGCTGTGG TACTTCGTGA AGTGCATTTA CTTTGCCCTG
    TCCGCCTACC AGATCCGCTG TGGCTACCCC ACCCGTATCT TGGGCAACTT CCTCACCAAG
    AAGTACAATC ATCTAAACCT CTTCCTTTTC CAGGGGTTCC GTCTAGTGCC ATTCCTGGTG
    GAGCTGCGGG CCGTCATGGA CTGGGTATGG ACTGACACCA CGCTGTCCCT GTCCAACTGG
    ATGTGTGTGG AAGACATCTA TGCCAACATC TTTATCATCA AGTGCAGCCG AGAGACAGAG
    AAGAAATACC CCCAGCCCAA GGGACAGAAG AAGAAGAAAA TCGTCAAGTA TGGCATGGGA
    GGTCTCATCA TCCTCTTCCT CATTGCCATC ATCTGGTTCC CTCTGCTCTT CATGTCACTG
    ATCCGCTCTG TGGTTGGTGT CGTCAACCAG CCCATTGATG TCACTGTTAC CCTCAAGTTA
    GGTGGCTACG AGCCACTGTT CACCATGAGC GCCCAGCAGC CATCCATAGT GCCATTCACT
    CCCGAGGACT ATGAGGAGCT GTCCCAGCAG TTTGACCCCT ACCCACTAGC CATGCAGTTC
    ATTAGCCAGT ACAGTCCTGA GGACATCGTC ACTGCACAGA TTGAGGGCAG CTCGGGGGCC
    CTGTGGCGCA TCAGCCCGCC CAGCCGGGCC CAGATGAAGC ACGAGCTGTA CAACGGCACA
    GCCGACATCA CACTGCGCTT CACCTGGAAT TTCCAAAGGG ACCTGGCCAA GGGCGGCTCT
    GTGGAGTATA CCAACGAGAA GCACACCTTG GAGCTGGCCC CCAACAGTAC GGCACGAAGG
    CAGCTGGCCC AACTGCTCGA GGGCAGACCT GACCAGTCAG TGGTCATTCC CCACCTCTTC
    CCCAAGTACA TTCGTGCACC CAATGGGCCT GAAGCCAATC CTGTGAAGCA ACTGCAGCCA
    GACGAGGAAG AGGACTACCT TGGTGTGCGT ATCCAGCTGC GGAGAGAGCA AGTGGGCACA
    GGAACCTCTG GGGAGCAAGC AGGCACAAAG GCCTCCGACT TCCTCGAGTG GTGGGTGATT
    GAGCTGCAGG ACTGCCAGGC CGAGTGCAAC CTGCTGCCCA TGGTCATCTT CAGTGACAAG
    GTCAGCCCGC CTAGCCTGGG CTTCCTGGCC GGCTACGGGA TTGTGGGGCT CTACGTCTCT
    ATCGTGCTGG TCGTTGGCAA GTTTGTGCGG GGCTTCTTCA GTGATATCTC ACACTCCATC
    ATGTTCGAGG AGCTGCCGTG CGTGGACCGC ATCCTCAAGC TGTGCCAGGA CATCTTCTTG
    GTACGGGAGA CCCGCGAGCT GGAGCTGGAG GAGGAACTCT ATGCCAAGCT CATATTCCTG
    TACCGATCAC CAGAGACCAT GATCAAGTGG ACGCGTGAGA AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001070668.2
    CDS291..7898
    Translation

    Target ORF information:

    RefSeq Version NM_001077200.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001077200.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG
    CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC
    CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGTAACCT TTGGGCAAAC
    GTGTCTCAAC ACATAGGGGT TACAAGGCTG GACCTGAAGG ACATCTTTAA CACCACCAGA
    CTGGTGGCAC CTGACCTGGG AGTGCTGGTG GCATCTTCCC TTTGCCTTGG CCTCTGTGGA
    CGCCTCACAA GGAAAGCCCG GCAGAGCCAG CGCACTCAGG AGCTGGAGGA GGATGACATA
    GATGCTGCCC CCGCCGCAGG GCTGCAGGGA GCCCCCACCC TGGCAACCAA ACGCAGGCTG
    TGGCTGGCCC CTCGCTTCCG GATCACGGCG CACTGGCTGC TGGTGACCTC CGGACGGATG
    CTGGTCATCG TGCTACTGGC ACTCGCAGGC ATAGCCCATC CTTCGGCCTT CTCCAGCGTC
    TACCTGATGG TGTTCCTGGC CATCTGCACC TGGTGGTCTT GCCACTTTCC TCTCAGCTCC
    CTGGGCTTTA ACACCCTCTG CGTCATGGTG AGCTGCTTTG GCGCCGGCCA TCTTGTTTGT
    TTATACTGCT ATCAGACACC ATTTGTCCAG AGCGTGCTAC TTCCTGGCAG CCTCTGGGCC
    AGGCTATTTG GTCTCAAGAA CTTCGTAGAC ATCCCTAACT GCTCCAGCCC CAACGTGCTG
    GTACTCAACA CTAAGCATGC TTGGCCCATC TACGTGAGCC CCGGTATCCT GCTGCTGTTG
    TACTACACAG CCACCTCTCT CCTGAAGCTC CGCAAGGGCC GCTTCTCAGA GCTGAGGAAG
    GAGATCCCGA GGGAGGATGA GGAGCATGAA CTGGAACTGG ACCAGCTGGA GCCAGAGCCC
    CAGGCTAGGG GCACCACCCA GGGTGCGACG CCCACGACCA CTGGACCCGA CATTGACAAC
    TGCACCGTGC ATGTACTAAC CAGCCAGAGC CCCGTCCGCC AGCGTCCTGT ACGCCCCAGG
    CTGGCTGAGC TGAAAGAGAT GTCACCACTA CATGGCCTGG GCCACCTCAT CCTGGACCAG
    AGCTATGTGT GTGCTCTGAT CGCCATGATG GTGTGGAGCA TCATGTACCA CAGCTGGCTG
    ACCTTCGTCT TGCTGCTCTG GGCCTGCCTC ATCTGGACCG TGCGCAGCCG CCACCAGTTG
    GCTATGCTGT GCTCACCCTG CATCCTGCTG TACGGACTGA CACTCTGCTG CCTGCGGTAC
    GTGTGGGCCA TGGAGCTCCC CGAGCTGCCC ACCACCCTGG GCCCTGTCAG TCTACATCAG
    TTGGGATTGG AGCATACACG CTACCCCTGC CTGGACCTTG GTGCCATGCT GCTGTATCTG
    CTCACGTTCT GGCTGCTCCT GCGTCAGTTC GTGAAGGAGA AGCTGCTGAA GAAGAGAAAG
    GCACCTTCGA CGCTGCTGGA GGTCACAGTG TCCGACACAG AGCCCACACA GACCCAGACA
    CTGCTGAGGA GCCTGGGGGA GCTGGTCACG GGCATCTACG TCAAGTACTG GATCTACGTG
    TGCGCTGGGA TGTTCATTGT GGTCAGCTTC GCTGGCCGCC TGGTGGTCTA CAAAATCGTC
    TACATGTTCC TTTTCCTGCT GTGCCTCACG TTGTTCCAGG TCTACTACAC TCTGTGGAGG
    AAGCTGCTCC GAGTTTTCTG GTGGCTCGTG GTGGCCTACA CCATGCTCGT GCTCATCGCC
    GTGTACACTT TCCAGTTCCA GGACTTCCCC ACCTACTGGC GCAACCTCAC GGGCTTCACA
    GATGAACAGT TGGGCGACCT GGGCCTGGAG CAGTTCAGCG TGTCGGAGCT CTTTTCCAGC
    ATCCTCATCC CTGGCTTCTT CCTGCTGGCC TGCATCCTGC AGCTGCACTA CTTCCACAGG
    CCGTTCATGC AGCTCACCGA CCTGGAGCAC GTGCCCCCGC CAGGCACACG CCGCCTTCGC
    TGGGCTCACA GGCAGGATAC AGTGAGCGAG GCCCCTCTGC TTCAGCATCA GGAGGAGGAG
    GAAGTCTTCA GGGATGACGG GCAGAGCATG GATGGGCCCC ACCAGACCAC ACAGGTCCCT
    GAGGGTACAG CCAGCAAGTG GGGCCTGGTG GCCGACCGGC TGCTGGACCT AGCGTCCAGC
    TTCTCCGCCG TCCTCACCCG CATCCAGGTG TTCGTACGAT GTTTGCTCGA GCTTCACGTC
    TTCAAGCTGG TGGCTCTCTA CACTGTCTGG GTGGCCCTGA AAGAAGTGTC CGTGATGAAC
    CTGCTGCTGG TGGTGCTGTG GGCCTTCGCC CTGCCCTACC CGCGCTTCCG GCCCATGGCC
    TCCTGCCTGT CCACCGTGTG GACCTGTATC ATCATCGTGT GCAAGATGCT GTATCAGCTG
    AAGATTGTCA ACCCACACGA GTACTCCAGT AACTGCACCG AGCCCTTTCC CAACAATACC
    AACTTGCAGC CCTTGGAGAT CAGCCAGTCT CTGCTGTACC GTGGCCCCGT TGACCCTGCC
    AACTGGTTTG GGGTGCGGAA GGGTTACCCA AACTTGGGCT ATATCCAGAA CCACCTGCAG
    ATCCTTCTGC TGCTGGTGTT TGAGGCTGTG GTGTACCGGC GCCAAGAGCA CTACCGCCGG
    CAGCACCAGC AGGCCCCTCT GCCCGCCCAG GCTTTGTGCG CTGATGGCAC CCGCCAGAGG
    CTGGACCAGG ACCTACTCAG CTGCCTCAAA TATTTCATCA ACTTCTTCTT CTACAAATTC
    GGGCTGGAGA TCTGCTTCTT GATGGCCGTG AATGTCATCG GACAACGCAT GAACTTCATG
    GTGATCTTAC ACGGCTGCTG GTTGGTGGCC ATCCTTACAC GCCGGCGCCG TGAGGCCATC
    GCCCGCCTCT GGCCCAATTA CTGTCTGTTC CTCACGCTGT TCCTGCTGTA CCAGTACCTG
    CTGTGCCTGG GCATGCCTCC CGCACTGTGC ATCGACTACC CGTGGCGCTG GAGCCAGGCC
    ATCCCCATGA ATTCTGCGCT CATCAAGTGG CTGTACCTCC CTGACTTCTT CAGGGCCCCC
    AACTCCACCA ACCTTATCAG TGACTTCCTC CTGCTACTCT GCGCCTCCCA ACAGTGGCAG
    GTGTTCTCCG CAGAGCAAAC GGAAGAATGG CAGCGCATGG CTGGCGTTAA CACTGACCAC
    CTGGAGCCCC TGCGTGGGGA GCCCAACCCT ATCCCCAACT TCATCCACTG CAGGTCCTAT
    CTGGATATGC TGAAGGTGGC CGTCTTCCGC TACCTGTTCT GGCTGGTGCT GGTGGTCGTG
    TTTGTCACAG GGGCCACCCG CATCAGCATC TTTGGGCTGG GGTACCTGCT GGCCTGCTTC
    TACCTGCTGC TCTTTGGCAC CACCCTGCTG CAGAAGGACA CACGAGCCCA GCTCGTGCTG
    TGGGACTGCC TCATCCTCTA CAACGTCACC GTCATCATCT CTAAGAATAT GTTGTCGCTC
    CTGTCCTGTG TCTTCGTGGA GCAAATGCAG AGCAACTTCT GCTGGGTCAT CCAGCTCTTC
    AGCCTCGTGT GCACAGTCAA AGGCTACTAT GACCCCAAAG AGATGAAGAC CAGGGACCGG
    GATTGCCTGC TGCCTGTGGA GGAGGCCGGG ATCATCTGGG ACAGCATCTG CTTCTTCTTC
    CTGCTCCTAC AACGGCGCGT CTTTCTCAGC CACTACTTCC TGCACGTCAG TGCTGACCTG
    AAGGCCACGG CCCTGCAGGC CTCCAGGGGT TTTGCTCTCT ACAATGCGGC CAACATAAAG
    AACATCAACT TCCACCGTCA GACCGAGGAG AGGTCCCTGG CCCAGCTGAA AAGACAGATG
    AAGCGCATCC GTGCCAAACA GGAGAAATAC CGGCAGAGCC AGGCAAGCCG TGGTCAACTC
    CAGTCCACAG ACCCTCAGGA GCCAGGGCCT GACAGCCCAG GGGGTTCCTC CCCGCCACGG
    ACACAGTGGT GGCGCCCCTG GCAGGACCAC GCCACAGTCA TCCACTCTGG TGACTACTTC
    CTGTTTGAGT CGGACAGTGA GGAGGAAGAG GAGGCCCTGC CTGAGGACCC CAGGCCTGCA
    GCTCAGAGTG CCTTCCAGAT GGCATACCAG GCATGGGTAA CCAACGCCCA GACAGTGCTA
    AGGCAGCGTC GTGAACAGGC ACGGCGGGAT CGGGCAGAAC AGCTGGCTTC TGGAGGCGAC
    TTGAGTCCTG AGGTGGAGCT GGTAGACGTC CCAGAGAATG AGATGGCAGG CCATAGCCAT
    GTGATGCAGC GCGTGCTGAG TACCATGCAG TTCCTGTGGG TGCTGGGCCA GGCCACAGTG
    GACGGGCTGA CGCGCTGGTT GCGTACGTTC ACGAAGGACC ACCGCACCAT GAGTGATGTG
    CTGTGTGCAG AGCGTTACCT GCTCACCCAG GAGCTTCTTC GGGGTGGAGA GGTACACCGA
    GGGGTACTGG ACCAGCTTTA CGTGAGCGAA GATGAGATCG CACTGTCAGG TCCTGTGGAG
    AACCGTGATG GACCCAGCAC AGCCTCTAGC GGGCTGGGAG CTGAAGAGCC TTTGAGTAGC
    ATGACAGACG ACACCGGCAG CCCCCTTAGC ACTGGCTACA ACACTCGCAG TGGTAGTGAG
    GAGATTATCA CCGACACTGG GGGCCTCCAG GCTGGGACCT CCCTGCACGG CTCCCAAGAG
    CTTCTAGCCA ATGCTCGTAC CCGGATGCGC ACAGCCAGTG AGCTGCTGCT GGACAGGCGC
    CTGCGTATCC CTGAGCTGGA GGAGGCCGAG CAGTTTGAGG CACAGCAGGG CCGGACGCTG
    CGGCTGCTCA GAGCCATGTA CCAGTGTGTG GCGGCCCACT CAGAGCTTCT CTGTTACTTC
    ATCATCATCC TTAACCACAT GGTGACAGCC TCGGCTGCCT CCCTGGTGCT GCCTGTGCTT
    GTGTTCCTCT GGGCCATGCT GACCATCCCG AGGCCTAGCA AGCGCTTCTG GATGACAGCT
    ATCGTCTTCA CTGAGGTCAT GGTGGTCACC AAATACTTGT TCCAGTTTGG CTTCTTCCCC
    TGGAACAGCT ACATAGTGCT TCGACGCTAC GAGAACAAGC CCTACTTCCC TCCGCGCATC
    CTGGGCCTCG AGAAAACAGA CAGCTATATC AAGTACGACC TGGTGCAACT CATGGCCCTC
    TTCTTCCACC GCTCGCAGTT ACTGTGCTAT GGCCTCTGGG ACCATGAGGA GGATGGTGTC
    CCCAAGGACC ATTGCAGGAG TAGCGAAAAG GACCAGGAAG CTGAGGAAGA GTCAGAGGCT
    AAGCTGGAAT CGCAGCCTGA GACGGGCACT GGGCATCCTG AGGAGCCCGT GTTGACTGGT
    ACCCCCAAGG ACCACATCCA AGGGAAAGGA AGTGTTAGGT CCAAGGATGA GATCCAAGAT
    CCCCCAGAGG ACCTTAAGCC CCAGCACAGG AGACACATCA GCATACGCTT CAGGAGGAGG
    AAGGAGACTC AAGGACCCAA AGGAGCAGCA GTCGTGGAGG CTGAGCACGA GGAGGGAGAG
    GAAGGAAGAG AAGCTGCAGG GAGAAAGAGG CTGCGTCGCC CTCGAGAAGG GTTGAAGATT
    CGGGAGAAAA TGAAGGCAGC TGGACGCCGG CTGCAGAGCT TCTGTCTGTC ACTGGCCCAG
    AGCTTTTACC AACCCCTGCG GCGTTTCTTC CATGACATTC TGCACACCAA GTACCGAGCG
    GCCACCGACG TCTATGCCCT CATGTTCCTG GCTGACATTG TCGACATTGT CGTCATCATC
    TTCGGTTTTT GGGCTTTTGG GAAGCACTCC GCGGCCACGG ACATTGCATC CTCACTGTCA
    GATGACCAGG TGCCACAGGC CTTCCTGTTC ATGCTGCTGG TCCAGTTCGG CACCATGGTC
    ATAGACCGCG CCCTCTACCT GCGTAAGACT GTACTGGGCA ACCTGGCCTT TCAGGTGGTC
    CTGGTGGTGG CCATTCACCT CTGGATGTTT TTCATCTTAC CGGCTGTCAC CGAGAGGATG
    TTCAGACAGA ACGCGGTGGC CCAGCTGTGG TACTTCGTGA AGTGCATTTA CTTTGCCCTG
    TCCGCCTACC AGATCCGCTG TGGCTACCCC ACCCGTATCT TGGGCAACTT CCTCACCAAG
    AAGTACAATC ATCTAAACCT CTTCCTTTTC CAGGGGTTCC GTCTAGTGCC ATTCCTGGTG
    GAGCTGCGGG CCGTCATGGA CTGGGTATGG ACTGACACCA CGCTGTCCCT GTCCAACTGG
    ATGTGTGTGG AAGACATCTA TGCCAACATC TTTATCATCA AGTGCAGCCG AGAGACAGAG
    AAGAAATACC CCCAGCCCAA GGGACAGAAG AAGAAGAAAA TCGTCAAGTA TGGCATGGGA
    GGTCTCATCA TCCTCTTCCT CATTGCCATC ATCTGGTTCC CTCTGCTCTT CATGTCACTG
    ATCCGCTCTG TGGTTGGTGT CGTCAACCAG CCCATTGATG TCACTGTTAC CCTCAAGTTA
    GGTGGCTACG AGCCACTGTT CACCATGAGC GCCCAGCAGC CATCCATAGT GCCATTCACT
    CCCGAGGACT ATGAGGAGCT GTCCCAGCAG TTTGACCCCT ACCCACTAGC CATGCAGTTC
    ATTAGCCAGT ACAGTCCTGA GGACATCGTC ACTGCACAGA TTGAGGGCAG CTCGGGGGCC
    CTGTGGCGCA TCAGCCCGCC CAGCCGGGCC CAGATGAAGC ACGAGCTGTA CAACGGCACA
    GCCGACATCA CACTGCGCTT CACCTGGAAT TTCCAAAGGG ACCTGGCCAA GGGCGGCTCT
    GTGGAGTATA CCAACGAGAA GCACACCTTG GAGCTGGCCC CCAACAGTAC GGCACGAAGG
    CAGCTGGCCC AACTGCTCGA GGGCAGACCT GACCAGTCAG TGGTCATTCC CCACCTCTTC
    CCCAAGTACA TTCGTGCACC CAATGGGCCT GAAGCCAATC CTGTGAAGCA ACTGCAGCCA
    GACGAGGAAG AGGACTACCT TGGTGTGCGT ATCCAGCTGC GGAGAGAGCA AGTGGGCACA
    GGAACCTCTG GGGAGCAAGC AGGCACAAAG GCCTCCGACT TCCTCGAGTG GTGGGTGATT
    GAGCTGCAGG ACTGCCAGGC CGAGTGCAAC CTGCTGCCCA TGGTCATCTT CAGTGACAAG
    GTCAGCCCGC CTAGCCTGGG CTTCCTGGCC GGCTACGGGA TTGTGGGGCT CTACGTCTCT
    ATCGTGCTGG TCGTTGGCAA GTTTGTGCGG GGCTTCTTCA GTGATATCTC ACACTCCATC
    ATGTTCGAGG AGCTGCCGTG CGTGGACCGC ATCCTCAAGC TGTGCCAGGA CATCTTCTTG
    GTACGGGAGA CCCGCGAGCT GGAGCTGGAG GAGGAACTCT ATGCCAAGCT CATATTCCTG
    TACCGATCAC CAGAGACCAT GATCAAGTGG ACGCGTGAGA AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa47912
    Clone ID Related Accession (Same CDS sequence) XM_008772626.2
    Accession Version XM_008772626.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG
    CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC
    CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGCAGCAG CCTTTGGGCA
    AACGTGTCTC AACACATAGG GGTTACAAGG CTGGACCTGA AGGACATCTT TAACACCACC
    AGACTGGTGG CACCTGACCT GGGAGTGCTG GTGGCATCTT CCCTTTGCCT TGGCCTCTGT
    GGACGCCTCA CAAGGAAAGC CCGGCAGAGC CAGCGCACTC AGGAGCTGCA GGAGGAGGAT
    GACATAGATG CTGCCCCCGC CGCAGGGCTG CAGGGAGCCC CCACCCTGGC AACCAAACGC
    AGGCTGTGGC TGGCCCCTCG CTTCCGGATC ACGGCGCACT GGCTGCTGGT GACCTCCGGA
    CGGATGCTGG TCATCGTGCT ACTGGCACTC GCAGGCATAG CCCATCCTTC GGCCTTCTCC
    AGCGTCTACC TGATGGTGTT CCTGGCCATC TGCACCTGGT GGTCTTGCCA CTTTCCTCTC
    AGCTCCCTGG GCTTTAACAC CCTCTGCGTC ATGGTGAGCT GCTTTGGCGC CGGCCATCTT
    GTTTGTTTAT ACTGCTATCA GACACCATTT GTCCAGAGCG TGCTACTTCC TGGCAGCCTC
    TGGGCCAGGC TATTTGGTCT CAAGAACTTC GTAGACATCC CTAACTGCTC CAGCCCCAAC
    GTGCTGGTAC TCAACACTAA GCATGCTTGG CCCATCTACG TGAGCCCCGG TATCCTGCTG
    CTGTTGTACT ACACAGCCAC CTCTCTCCTG AAGCTCCGCA AGGGCCGCTT CTCAGAGCTG
    AGGAAGGAGA TCCCGAGGGA GGATGAGGAG CATGAACTGG AACTGGACCA GCTGGAGCCA
    GAGCCCCAGG CTAGGGGCAC CACCCAGGGT GCGACGCCCA CGACCACTGG ACCCGACATT
    GACAACTGCA CCGTGCATGT ACTAACCAGC CAGAGCCCCG TCCGCCAGCG TCCTGTACGC
    CCCAGGCTGG CTGAGCTGAA AGAGATGTCA CCACTACATG GCCTGGGCCA CCTCATCCTG
    GACCAGAGCT ATGTGTGTGC TCTGATCGCC ATGATGGTGT GGAGCATCAT GTACCACAGC
    TGGCTGACCT TCGTCTTGCT GCTCTGGGCC TGCCTCATCT GGACCGTGCG CAGCCGCCAC
    CAGTTGGCTA TGCTGTGCTC ACCCTGCATC CTGCTGTACG GACTGACACT CTGCTGCCTG
    CGGTACGTGT GGGCCATGGA GCTCCCCGAG CTGCCCACCA CCCTGGGCCC TGTCAGTCTA
    CATCAGTTGG GATTGGAGCA TACACGCTAC CCCTGCCTGG ACCTTGGTGC CATGCTGCTG
    TATCTGCTCA CGTTCTGGCT GCTCCTGCGT CAGTTCGTGA AGGAGAAGCT GCTGAAGAAG
    AGAAAGGCAC CTTCGACGCT GCTGGAGGTC ACAGTGTCCG ACACAGAGCC CACACAGACC
    CAGACACTGC TGAGGAGCCT GGGGGAGCTG GTCACGGGCA TCTACGTCAA GTACTGGATC
    TACGTGTGCG CTGGGATGTT CATTGTGGTC AGCTTCGCTG GCCGCCTGGT GGTCTACAAA
    ATCGTCTACA TGTTCCTTTT CCTGCTGTGC CTCACGTTGT TCCAGGTCTA CTACACTCTG
    TGGAGGAAGC TGCTCCGAGT TTTCTGGTGG CTCGTGGTGG CCTACACCAT GCTCGTGCTC
    ATCGCCGTGT ACACTTTCCA GTTCCAGGAC TTCCCCACCT ACTGGCGCAA CCTCACGGGC
    TTCACAGATG AACAGTTGGG CGACCTGGGC CTGGAGCAGT TCAGCGTGTC GGAGCTCTTT
    TCCAGCATCC TCATCCCTGG CTTCTTCCTG CTGGCCTGCA TCCTGCAGCT GCACTACTTC
    CACAGGCCGT TCATGCAGCT CACCGACCTG GAGCACGTGC CCCCGCCAGG CACACGCCGC
    CTTCGCTGGG CTCACAGGCA GGATACAGTG AGCGAGGCCC CTCTGCTTCA GCATCAGGAG
    GAGGAGGAAG TCTTCAGGGA TGACGGGCAG AGCATGGATG GGCCCCACCA GACCACACAG
    GTCCCTGAGG GTACAGCCAG CAAGTGGGGC CTGGTGGCCG ACCGGCTGCT GGACCTAGCG
    TCCAGCTTCT CCGCCGTCCT CACCCGCATC CAGGTGTTCG TACGATGTTT GCTCGAGCTT
    CACGTCTTCA AGCTGGTGGC TCTCTACACT GTCTGGGTGG CCCTGAAAGA AGTGTCCGTG
    ATGAACCTGC TGCTGGTGGT GCTGTGGGCC TTCGCCCTGC CCTACCCGCG CTTCCGGCCC
    ATGGCCTCCT GCCTGTCCAC CGTGTGGACC TGTATCATCA TCGTGTGCAA GATGCTGTAT
    CAGCTGAAGA TTGTCAACCC ACACGAGTAC TCCAGTAACT GCACCGAGCC CTTTCCCAAC
    AATACCAACT TGCAGCCCTT GGAGATCAGC CAGTCTCTGC TGTACCGTGG CCCCGTTGAC
    CCTGCCAACT GGTTTGGGGT GCGGAAGGGT TACCCAAACT TGGGCTATAT CCAGAACCAC
    CTGCAGATCC TTCTGCTGCT GGTGTTTGAG GCTGTGGTGT ACCGGCGCCA AGAGCACTAC
    CGCCGGCAGC ACCAGCAGGC CCCTCTGCCC GCCCAGGCTT TGTGCGCTGA TGGCACCCGC
    CAGAGGCTGG ACCAGGACCT ACTCAGCTGC CTCAAATATT TCATCAACTT CTTCTTCTAC
    AAATTCGGGC TGGAGATCTG CTTCTTGATG GCCGTGAATG TCATCGGACA ACGCATGAAC
    TTCATGGTGA TCTTACACGG CTGCTGGTTG GTGGCCATCC TTACACGCCG GCGCCGTGAG
    GCCATCGCCC GCCTCTGGCC CAATTACTGT CTGTTCCTCA CGCTGTTCCT GCTGTACCAG
    TACCTGCTGT GCCTGGGCAT GCCTCCCGCA CTGTGCATCG ACTACCCGTG GCGCTGGAGC
    CAGGCCATCC CCATGAATTC TGCGCTCATC AAGTGGCTGT ACCTCCCTGA CTTCTTCAGG
    GCCCCCAACT CCACCAACCT TATCAGTGAC TTCCTCCTGC TACTCTGCGC CTCCCAACAG
    TGGCAGGTGT TCTCCGCAGA GCAAACGGAA GAATGGCAGC GCATGGCTGG CGTTAACACT
    GACCACCTGG AGCCCCTGCG TGGGGAGCCC AACCCTATCC CCAACTTCAT CCACTGCAGG
    TCCTATCTGG ATATGCTGAA GGTGGCCGTC TTCCGCTACC TGTTCTGGCT GGTGCTGGTG
    GTCGTGTTTG TCACAGGGGC CACCCGCATC AGCATCTTTG GGCTGGGGTA CCTGCTGGCC
    TGCTTCTACC TGCTGCTCTT TGGCACCACC CTGCTGCAGA AGGACACACG AGCCCAGCTC
    GTGCTGTGGG ACTGCCTCAT CCTCTACAAC GTCACCGTCA TCATCTCTAA GAATATGTTG
    TCGCTCCTGT CCTGTGTCTT CGTGGAGCAA ATGCAGAGCA ACTTCTGCTG GGTCATCCAG
    CTCTTCAGCC TCGTGTGCAC AGTCAAAGGC TACTATGACC CCAAAGAGAT GAAGACCAGG
    GACCGGGATT GCCTGCTGCC TGTGGAGGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC
    TTCTTCCTGC TCCTACAACG GCGCGTCTTT CTCAGCCACT ACTTCCTGCA CGTCAGTGCT
    GACCTGAAGG CCACGGCCCT GCAGGCCTCC AGGGGTTTTG CTCTCTACAA TGCGGCCAAC
    ATAAAGAACA TCAACTTCCA CCGTCAGACC GAGGAGAGGT CCCTGGCCCA GCTGAAAAGA
    CAGATGAAGC GCATCCGTGC CAAACAGGAG AAATACCGGC AGAGCCAGGC AAGCCGTGGT
    CAACTCCAGT CCACAGACCC TCAGGAGCCA GGGCCTGACA GCCCAGGGGG TTCCTCCCCG
    CCACGGACAC AGTGGTGGCG CCCCTGGCAG GACCACGCCA CAGTCATCCA CTCTGGTGAC
    TACTTCCTGT TTGAGTCGGA CAGTGAGGAG GAAGAGGAGG CCCTGCCTGA GGACCCCAGG
    CCTGCAGCTC AGAGTGCCTT CCAGATGGCA TACCAGGCAT GGGTAACCAA CGCCCAGACA
    GTGCTAAGGC AGCGTCGTGA ACAGGCACGG CGGGATCGGG CAGAACAGCT GGCTTCTGGA
    GGCGACTTGA GTCCTGAGGT GGAGCCGGTA GACGTCCCAG AGAATGAGAT GGCAGGCCGT
    AGCCATGTGA TGCAGCGCGT GCTGAGTACC ATGCAGTTCC TGTGGGTGCT GGGCCAGGCC
    ACAGTGGACG GGCTGACGCG CTGGTTGCGT ACGTTCACGA AGGACCACCG CACCATGAGT
    GATGTGCTGT GTGCAGAGCG TTACCTGCTC ACCCAGGAGC TTCTTCGGGG TGGAGAGGTA
    CACCGAGGGG TACTGGACCA GCTTTACGTG AGCGAAGATG AGATCGCACT GTCAGGTCCT
    GTGGAGAACC GTGATGGACC CAGCACAGCC TCTAGCGGGC TGGGAGCTGA AGAGCCTTTG
    AGTAGCATGA CAGACGACAC CGGCAGCCCC CTTAGCACTG GCTACAACAC TCGCAGTGGT
    AGTGAGGAGA TTATCACCGA CACTGGGGGC CTCCAGGCTG GGACCTCCCT GCACGGCTCC
    CAAGAGCTTC TAGCCAATGC TCGTACCCGG ATGCGCACAG CCAGTGAGCT GCTGCTGGAC
    AGGCGCCTGC GTATCCCTGA GCTGGAGGAG GCCGAGCAGT TTGAGGCACA GCAGGGCCGG
    ACGCTGCGGC TGCTCAGAGC CATGTACCAG TGTGTGGCGG CCCACTCAGA GCTTCTCTGT
    TACTTCATCA TCATCCTTAA CCACATGGTG ACAGCCTCGG CTGCCTCCCT GGTGCTGCCT
    GTGCTTGTGT TCCTCTGGGC CATGCTGACC ATCCCGAGGC CTAGCAAGCG CTTCTGGATG
    ACAGCTATCG TCTTCACTGA GGTCATGGTG GTCACCAAAT ACTTGTTCCA GTTTGGCTTC
    TTCCCCTGGA ACAGCTACAT AGTGCTTCGA CGCTACGAGA ACAAGCCCTA CTTCCCTCCG
    CGCATCCTGG GCCTCGAGAA AACGGACAGC TATATCAAGT ACGACCTGGT GCAACTCATG
    GCCCTCTTCT TCCACCGCTC GCAGTTACTG TGCTATGGCC TCTGGGACCA TGAGGAGGAT
    GGTGTCCCCA AGGACCATTG CAGGAGTAGC GAAAAGGACC AAGAAGCTGA GGAAGAGTCA
    GAGGCTAAGC TGGAATCGCA GCCTGAGACG GGCACTGGGC ATCCTGAGGA GCCCGTGTTG
    ACTGGTACCC CCAAGGACCA CATCCAAGGG AAAGGAAGTG TTAGGTCCAA GGATGAGATC
    CAAGATCCCC CAGAGGACCT TAAGCCCCAG CACAGGAGAC ACATCAGCAT ACGCTTCAGG
    AGGAGGAAGG AGACTCAAGG ACCCAAAGGA GCAGCAGTCG TGGAGGCTGA GCACGAGGAG
    GGAGAGGAAG GAAGAGAAGC TGCAGGGAGA AAGAGGCTGC GTCGCCCTCG AGAAGGGTTG
    AAGATTCGGG AGAAAATGAA GGCAGCTGGA CGCCGGCTGC AGAGCTTCTG TCTGTCACTG
    GCCCAGAGCT TTTACCAACC CCTGCGGCGT TTCTTCCATG ACATTCTGCA CACCAAGTAC
    CGAGCGGCCA CCGACGTCTA TGCCCTCATG TTCCTGGCTG ACATTGTCGA CATTGTCGTC
    ATCATCTTCG GTTTTTGGGC TTTTGGGAAG CACTCCGCGG CCACGGACAT TGCATCCTCA
    CTGTCAGATG ACCAGGTGCC ACAGGCCTTC CTGTTCATGC TGCTGGTCCA GTTCGGCACC
    ATGGTCATAG ACCGCGCCCT CTACCTGCGT AAGACTGTAC TGGGCAAGCT GGCCTTTCAG
    GTGGTCCTGG TGGTGGCCAT TCACCTCTGG ATGTTTTTCA TCTTACCGGC TGTCACCGAG
    AGGATGTTCA GACAGAACGC GGTGGCCCAG CTGTGGTACT TCGTGAAGTG CATTTACTTT
    GCCCTGTCCG CCTACCAGAT CCGCTGTGGC TACCCCACCC GTATCTTGGG CAACTTCCTC
    ACCAAGAAGT ACAATCATCT AAACCTCTTC CTTTTCCAGG GGTTCCGTCT AGTGCCATTC
    CTGGTGGAGC TGCGGGCCGT CATGGACTGG GTATGGACTG ACACCACGCT GTCCCTGTCC
    AACTGGATGT GTGTGGAAGA CATCTATGCC AACATCTTTA TCATCAAGTG CAGCCGAGAG
    ACAGAGAAGA AATACCCCCA GCCCAAGGGA CAGAAGAAGA AGAAAATCGT CAAGTATGGC
    ATGGGAGGTC TCATCATCCT CTTCCTCATT GCCATCATCT GGTTCCCTCT GCTCTTCATG
    TCACTGATCC GCTCTGTGGT TGGTGTCGTC AACCAGCCCA TTGATGTCAC TGTTACCCTC
    AAGTTAGGTG GCTACGAGCC ACTGTTCACC ATGAGCGCCC AGCAGCCATC CATAGTGCCA
    TTCACTCCCG AGGACTATGA GGAGCTGTCC CAGCAGTTTG ACCCCTACCC ACTAGCCATG
    CAGTTCATTA GCCAGTACAG TCCTGAGGAC ATCGTCACTG CACAGATTGA GGGCAGCTCG
    GGGGCCCTGT GGCGCATCAG CCCGCCCAGC CGGGCCCAGA TGAAGCACGA GCTGTACAAC
    GGCACAGCCG ACATCACACT GCGCTTCACC TGGAATTTCC AAAGGGACCT GGCCAAGGGC
    GGCTCTGTGG AGTATACCAA CGAGAAGCAC ACCTTGGAGC TGGCCCCCAA CAGTACGGCA
    CGAAGGCAGC TGGCCCAACT GCTCGAGGGC AGACCTGACC AGTCAGTGGT CATTCCCCAC
    CTCTTCCCCA AGTACATTCG TGCACCCAAT GGGCCTGAAG CCAATCCTGT GAAGCAACTG
    CAGCCAGACG AGGAAGAGGA CTACCTTGGT GTGCGTATCC AGCTGCGGAG AGAGCAAGTG
    GGCACAGGAA CCTCTGGGGA GCAAGCAGGC ACAAAGGCCT CCGACTTCCT CGAGTGGTGG
    GTGATTGAGC TGCAGGACTG CCAGGCCGAG TGCAACCTGC TGCCCATGGT CATCTTCAGT
    GACAAGGTCA GCCCGCCTAG CCTGGGCTTC CTGGCCGGCT ACGGGATTGT GGGGCTCTAC
    GTCTCTATCG TGCTGGTCGT TGGCAAGTTT GTGCGGGGCT TCTTCAGTGA TATCTCACAC
    TCCATCATGT TCGAGGAGCT GCCGTGCGTG GACCGCATCC TCAAGCTGTG CCAGGACATC
    TTCTTGGTAC GGGAGACCCG AGAGCTGGAG CTGGAGGAGG AACTCTATGC CAAGCTCATA
    TTCCTGTACC GATCACCAGA GACCATGATC AAGTGGACGC GTGAGAAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008770848.1
    CDS618..8231
    Translation

    Target ORF information:

    RefSeq Version XM_008772626.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008772626.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG
    CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC
    CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGCAGCAG CCTTTGGGCA
    AACGTGTCTC AACACATAGG GGTTACAAGG CTGGACCTGA AGGACATCTT TAACACCACC
    AGACTGGTGG CACCTGACCT GGGAGTGCTG GTGGCATCTT CCCTTTGCCT TGGCCTCTGT
    GGACGCCTCA CAAGGAAAGC CCGGCAGAGC CAGCGCACTC AGGAGCTGCA GGAGGAGGAT
    GACATAGATG CTGCCCCCGC CGCAGGGCTG CAGGGAGCCC CCACCCTGGC AACCAAACGC
    AGGCTGTGGC TGGCCCCTCG CTTCCGGATC ACGGCGCACT GGCTGCTGGT GACCTCCGGA
    CGGATGCTGG TCATCGTGCT ACTGGCACTC GCAGGCATAG CCCATCCTTC GGCCTTCTCC
    AGCGTCTACC TGATGGTGTT CCTGGCCATC TGCACCTGGT GGTCTTGCCA CTTTCCTCTC
    AGCTCCCTGG GCTTTAACAC CCTCTGCGTC ATGGTGAGCT GCTTTGGCGC CGGCCATCTT
    GTTTGTTTAT ACTGCTATCA GACACCATTT GTCCAGAGCG TGCTACTTCC TGGCAGCCTC
    TGGGCCAGGC TATTTGGTCT CAAGAACTTC GTAGACATCC CTAACTGCTC CAGCCCCAAC
    GTGCTGGTAC TCAACACTAA GCATGCTTGG CCCATCTACG TGAGCCCCGG TATCCTGCTG
    CTGTTGTACT ACACAGCCAC CTCTCTCCTG AAGCTCCGCA AGGGCCGCTT CTCAGAGCTG
    AGGAAGGAGA TCCCGAGGGA GGATGAGGAG CATGAACTGG AACTGGACCA GCTGGAGCCA
    GAGCCCCAGG CTAGGGGCAC CACCCAGGGT GCGACGCCCA CGACCACTGG ACCCGACATT
    GACAACTGCA CCGTGCATGT ACTAACCAGC CAGAGCCCCG TCCGCCAGCG TCCTGTACGC
    CCCAGGCTGG CTGAGCTGAA AGAGATGTCA CCACTACATG GCCTGGGCCA CCTCATCCTG
    GACCAGAGCT ATGTGTGTGC TCTGATCGCC ATGATGGTGT GGAGCATCAT GTACCACAGC
    TGGCTGACCT TCGTCTTGCT GCTCTGGGCC TGCCTCATCT GGACCGTGCG CAGCCGCCAC
    CAGTTGGCTA TGCTGTGCTC ACCCTGCATC CTGCTGTACG GACTGACACT CTGCTGCCTG
    CGGTACGTGT GGGCCATGGA GCTCCCCGAG CTGCCCACCA CCCTGGGCCC TGTCAGTCTA
    CATCAGTTGG GATTGGAGCA TACACGCTAC CCCTGCCTGG ACCTTGGTGC CATGCTGCTG
    TATCTGCTCA CGTTCTGGCT GCTCCTGCGT CAGTTCGTGA AGGAGAAGCT GCTGAAGAAG
    AGAAAGGCAC CTTCGACGCT GCTGGAGGTC ACAGTGTCCG ACACAGAGCC CACACAGACC
    CAGACACTGC TGAGGAGCCT GGGGGAGCTG GTCACGGGCA TCTACGTCAA GTACTGGATC
    TACGTGTGCG CTGGGATGTT CATTGTGGTC AGCTTCGCTG GCCGCCTGGT GGTCTACAAA
    ATCGTCTACA TGTTCCTTTT CCTGCTGTGC CTCACGTTGT TCCAGGTCTA CTACACTCTG
    TGGAGGAAGC TGCTCCGAGT TTTCTGGTGG CTCGTGGTGG CCTACACCAT GCTCGTGCTC
    ATCGCCGTGT ACACTTTCCA GTTCCAGGAC TTCCCCACCT ACTGGCGCAA CCTCACGGGC
    TTCACAGATG AACAGTTGGG CGACCTGGGC CTGGAGCAGT TCAGCGTGTC GGAGCTCTTT
    TCCAGCATCC TCATCCCTGG CTTCTTCCTG CTGGCCTGCA TCCTGCAGCT GCACTACTTC
    CACAGGCCGT TCATGCAGCT CACCGACCTG GAGCACGTGC CCCCGCCAGG CACACGCCGC
    CTTCGCTGGG CTCACAGGCA GGATACAGTG AGCGAGGCCC CTCTGCTTCA GCATCAGGAG
    GAGGAGGAAG TCTTCAGGGA TGACGGGCAG AGCATGGATG GGCCCCACCA GACCACACAG
    GTCCCTGAGG GTACAGCCAG CAAGTGGGGC CTGGTGGCCG ACCGGCTGCT GGACCTAGCG
    TCCAGCTTCT CCGCCGTCCT CACCCGCATC CAGGTGTTCG TACGATGTTT GCTCGAGCTT
    CACGTCTTCA AGCTGGTGGC TCTCTACACT GTCTGGGTGG CCCTGAAAGA AGTGTCCGTG
    ATGAACCTGC TGCTGGTGGT GCTGTGGGCC TTCGCCCTGC CCTACCCGCG CTTCCGGCCC
    ATGGCCTCCT GCCTGTCCAC CGTGTGGACC TGTATCATCA TCGTGTGCAA GATGCTGTAT
    CAGCTGAAGA TTGTCAACCC ACACGAGTAC TCCAGTAACT GCACCGAGCC CTTTCCCAAC
    AATACCAACT TGCAGCCCTT GGAGATCAGC CAGTCTCTGC TGTACCGTGG CCCCGTTGAC
    CCTGCCAACT GGTTTGGGGT GCGGAAGGGT TACCCAAACT TGGGCTATAT CCAGAACCAC
    CTGCAGATCC TTCTGCTGCT GGTGTTTGAG GCTGTGGTGT ACCGGCGCCA AGAGCACTAC
    CGCCGGCAGC ACCAGCAGGC CCCTCTGCCC GCCCAGGCTT TGTGCGCTGA TGGCACCCGC
    CAGAGGCTGG ACCAGGACCT ACTCAGCTGC CTCAAATATT TCATCAACTT CTTCTTCTAC
    AAATTCGGGC TGGAGATCTG CTTCTTGATG GCCGTGAATG TCATCGGACA ACGCATGAAC
    TTCATGGTGA TCTTACACGG CTGCTGGTTG GTGGCCATCC TTACACGCCG GCGCCGTGAG
    GCCATCGCCC GCCTCTGGCC CAATTACTGT CTGTTCCTCA CGCTGTTCCT GCTGTACCAG
    TACCTGCTGT GCCTGGGCAT GCCTCCCGCA CTGTGCATCG ACTACCCGTG GCGCTGGAGC
    CAGGCCATCC CCATGAATTC TGCGCTCATC AAGTGGCTGT ACCTCCCTGA CTTCTTCAGG
    GCCCCCAACT CCACCAACCT TATCAGTGAC TTCCTCCTGC TACTCTGCGC CTCCCAACAG
    TGGCAGGTGT TCTCCGCAGA GCAAACGGAA GAATGGCAGC GCATGGCTGG CGTTAACACT
    GACCACCTGG AGCCCCTGCG TGGGGAGCCC AACCCTATCC CCAACTTCAT CCACTGCAGG
    TCCTATCTGG ATATGCTGAA GGTGGCCGTC TTCCGCTACC TGTTCTGGCT GGTGCTGGTG
    GTCGTGTTTG TCACAGGGGC CACCCGCATC AGCATCTTTG GGCTGGGGTA CCTGCTGGCC
    TGCTTCTACC TGCTGCTCTT TGGCACCACC CTGCTGCAGA AGGACACACG AGCCCAGCTC
    GTGCTGTGGG ACTGCCTCAT CCTCTACAAC GTCACCGTCA TCATCTCTAA GAATATGTTG
    TCGCTCCTGT CCTGTGTCTT CGTGGAGCAA ATGCAGAGCA ACTTCTGCTG GGTCATCCAG
    CTCTTCAGCC TCGTGTGCAC AGTCAAAGGC TACTATGACC CCAAAGAGAT GAAGACCAGG
    GACCGGGATT GCCTGCTGCC TGTGGAGGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC
    TTCTTCCTGC TCCTACAACG GCGCGTCTTT CTCAGCCACT ACTTCCTGCA CGTCAGTGCT
    GACCTGAAGG CCACGGCCCT GCAGGCCTCC AGGGGTTTTG CTCTCTACAA TGCGGCCAAC
    ATAAAGAACA TCAACTTCCA CCGTCAGACC GAGGAGAGGT CCCTGGCCCA GCTGAAAAGA
    CAGATGAAGC GCATCCGTGC CAAACAGGAG AAATACCGGC AGAGCCAGGC AAGCCGTGGT
    CAACTCCAGT CCACAGACCC TCAGGAGCCA GGGCCTGACA GCCCAGGGGG TTCCTCCCCG
    CCACGGACAC AGTGGTGGCG CCCCTGGCAG GACCACGCCA CAGTCATCCA CTCTGGTGAC
    TACTTCCTGT TTGAGTCGGA CAGTGAGGAG GAAGAGGAGG CCCTGCCTGA GGACCCCAGG
    CCTGCAGCTC AGAGTGCCTT CCAGATGGCA TACCAGGCAT GGGTAACCAA CGCCCAGACA
    GTGCTAAGGC AGCGTCGTGA ACAGGCACGG CGGGATCGGG CAGAACAGCT GGCTTCTGGA
    GGCGACTTGA GTCCTGAGGT GGAGCCGGTA GACGTCCCAG AGAATGAGAT GGCAGGCCGT
    AGCCATGTGA TGCAGCGCGT GCTGAGTACC ATGCAGTTCC TGTGGGTGCT GGGCCAGGCC
    ACAGTGGACG GGCTGACGCG CTGGTTGCGT ACGTTCACGA AGGACCACCG CACCATGAGT
    GATGTGCTGT GTGCAGAGCG TTACCTGCTC ACCCAGGAGC TTCTTCGGGG TGGAGAGGTA
    CACCGAGGGG TACTGGACCA GCTTTACGTG AGCGAAGATG AGATCGCACT GTCAGGTCCT
    GTGGAGAACC GTGATGGACC CAGCACAGCC TCTAGCGGGC TGGGAGCTGA AGAGCCTTTG
    AGTAGCATGA CAGACGACAC CGGCAGCCCC CTTAGCACTG GCTACAACAC TCGCAGTGGT
    AGTGAGGAGA TTATCACCGA CACTGGGGGC CTCCAGGCTG GGACCTCCCT GCACGGCTCC
    CAAGAGCTTC TAGCCAATGC TCGTACCCGG ATGCGCACAG CCAGTGAGCT GCTGCTGGAC
    AGGCGCCTGC GTATCCCTGA GCTGGAGGAG GCCGAGCAGT TTGAGGCACA GCAGGGCCGG
    ACGCTGCGGC TGCTCAGAGC CATGTACCAG TGTGTGGCGG CCCACTCAGA GCTTCTCTGT
    TACTTCATCA TCATCCTTAA CCACATGGTG ACAGCCTCGG CTGCCTCCCT GGTGCTGCCT
    GTGCTTGTGT TCCTCTGGGC CATGCTGACC ATCCCGAGGC CTAGCAAGCG CTTCTGGATG
    ACAGCTATCG TCTTCACTGA GGTCATGGTG GTCACCAAAT ACTTGTTCCA GTTTGGCTTC
    TTCCCCTGGA ACAGCTACAT AGTGCTTCGA CGCTACGAGA ACAAGCCCTA CTTCCCTCCG
    CGCATCCTGG GCCTCGAGAA AACGGACAGC TATATCAAGT ACGACCTGGT GCAACTCATG
    GCCCTCTTCT TCCACCGCTC GCAGTTACTG TGCTATGGCC TCTGGGACCA TGAGGAGGAT
    GGTGTCCCCA AGGACCATTG CAGGAGTAGC GAAAAGGACC AAGAAGCTGA GGAAGAGTCA
    GAGGCTAAGC TGGAATCGCA GCCTGAGACG GGCACTGGGC ATCCTGAGGA GCCCGTGTTG
    ACTGGTACCC CCAAGGACCA CATCCAAGGG AAAGGAAGTG TTAGGTCCAA GGATGAGATC
    CAAGATCCCC CAGAGGACCT TAAGCCCCAG CACAGGAGAC ACATCAGCAT ACGCTTCAGG
    AGGAGGAAGG AGACTCAAGG ACCCAAAGGA GCAGCAGTCG TGGAGGCTGA GCACGAGGAG
    GGAGAGGAAG GAAGAGAAGC TGCAGGGAGA AAGAGGCTGC GTCGCCCTCG AGAAGGGTTG
    AAGATTCGGG AGAAAATGAA GGCAGCTGGA CGCCGGCTGC AGAGCTTCTG TCTGTCACTG
    GCCCAGAGCT TTTACCAACC CCTGCGGCGT TTCTTCCATG ACATTCTGCA CACCAAGTAC
    CGAGCGGCCA CCGACGTCTA TGCCCTCATG TTCCTGGCTG ACATTGTCGA CATTGTCGTC
    ATCATCTTCG GTTTTTGGGC TTTTGGGAAG CACTCCGCGG CCACGGACAT TGCATCCTCA
    CTGTCAGATG ACCAGGTGCC ACAGGCCTTC CTGTTCATGC TGCTGGTCCA GTTCGGCACC
    ATGGTCATAG ACCGCGCCCT CTACCTGCGT AAGACTGTAC TGGGCAAGCT GGCCTTTCAG
    GTGGTCCTGG TGGTGGCCAT TCACCTCTGG ATGTTTTTCA TCTTACCGGC TGTCACCGAG
    AGGATGTTCA GACAGAACGC GGTGGCCCAG CTGTGGTACT TCGTGAAGTG CATTTACTTT
    GCCCTGTCCG CCTACCAGAT CCGCTGTGGC TACCCCACCC GTATCTTGGG CAACTTCCTC
    ACCAAGAAGT ACAATCATCT AAACCTCTTC CTTTTCCAGG GGTTCCGTCT AGTGCCATTC
    CTGGTGGAGC TGCGGGCCGT CATGGACTGG GTATGGACTG ACACCACGCT GTCCCTGTCC
    AACTGGATGT GTGTGGAAGA CATCTATGCC AACATCTTTA TCATCAAGTG CAGCCGAGAG
    ACAGAGAAGA AATACCCCCA GCCCAAGGGA CAGAAGAAGA AGAAAATCGT CAAGTATGGC
    ATGGGAGGTC TCATCATCCT CTTCCTCATT GCCATCATCT GGTTCCCTCT GCTCTTCATG
    TCACTGATCC GCTCTGTGGT TGGTGTCGTC AACCAGCCCA TTGATGTCAC TGTTACCCTC
    AAGTTAGGTG GCTACGAGCC ACTGTTCACC ATGAGCGCCC AGCAGCCATC CATAGTGCCA
    TTCACTCCCG AGGACTATGA GGAGCTGTCC CAGCAGTTTG ACCCCTACCC ACTAGCCATG
    CAGTTCATTA GCCAGTACAG TCCTGAGGAC ATCGTCACTG CACAGATTGA GGGCAGCTCG
    GGGGCCCTGT GGCGCATCAG CCCGCCCAGC CGGGCCCAGA TGAAGCACGA GCTGTACAAC
    GGCACAGCCG ACATCACACT GCGCTTCACC TGGAATTTCC AAAGGGACCT GGCCAAGGGC
    GGCTCTGTGG AGTATACCAA CGAGAAGCAC ACCTTGGAGC TGGCCCCCAA CAGTACGGCA
    CGAAGGCAGC TGGCCCAACT GCTCGAGGGC AGACCTGACC AGTCAGTGGT CATTCCCCAC
    CTCTTCCCCA AGTACATTCG TGCACCCAAT GGGCCTGAAG CCAATCCTGT GAAGCAACTG
    CAGCCAGACG AGGAAGAGGA CTACCTTGGT GTGCGTATCC AGCTGCGGAG AGAGCAAGTG
    GGCACAGGAA CCTCTGGGGA GCAAGCAGGC ACAAAGGCCT CCGACTTCCT CGAGTGGTGG
    GTGATTGAGC TGCAGGACTG CCAGGCCGAG TGCAACCTGC TGCCCATGGT CATCTTCAGT
    GACAAGGTCA GCCCGCCTAG CCTGGGCTTC CTGGCCGGCT ACGGGATTGT GGGGCTCTAC
    GTCTCTATCG TGCTGGTCGT TGGCAAGTTT GTGCGGGGCT TCTTCAGTGA TATCTCACAC
    TCCATCATGT TCGAGGAGCT GCCGTGCGTG GACCGCATCC TCAAGCTGTG CCAGGACATC
    TTCTTGGTAC GGGAGACCCG AGAGCTGGAG CTGGAGGAGG AACTCTATGC CAAGCTCATA
    TTCCTGTACC GATCACCAGA GACCATGATC AAGTGGACGC GTGAGAAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa47913
    Clone ID Related Accession (Same CDS sequence) XM_008772627.2
    Accession Version XM_008772627.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG
    CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC
    CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGCAGCAG CCTTTGGGCA
    AACGTGTCTC AACACATAGG GGTTACAAGG CTGGACCTGA AGGACATCTT TAACACCACC
    AGACTGGTGG CACCTGACCT GGGAGTGCTG GTGGCATCTT CCCTTTGCCT TGGCCTCTGT
    GGACGCCTCA CAAGGAAAGC CCGGCAGAGC CAGCGCACTC AGGAGCTGCA GGAGGAGGAT
    GACATAGATG CTGCCCCCGC CGCAGGGCTG CAGGGAGCCC CCACCCTGGC AACCAAACGC
    AGGCTGTGGC TGGCCCCTCG CTTCCGGATC ACGGCGCACT GGCTGCTGGT GACCTCCGGA
    CGGATGCTGG TCATCGTGCT ACTGGCACTC GCAGGCATAG CCCATCCTTC GGCCTTCTCC
    AGCGTCTACC TGATGGTGTT CCTGGCCATC TGCACCTGGT GGTCTTGCCA CTTTCCTCTC
    AGCTCCCTGG GCTTTAACAC CCTCTGCGTC ATGGTGAGCT GCTTTGGCGC CGGCCATCTT
    GTTTGTTTAT ACTGCTATCA GACACCATTT GTCCAGAGCG TGCTACTTCC TGGCAGCCTC
    TGGGCCAGGC TATTTGGTCT CAAGAACTTC GTAGACATCC CTAACTGCTC CAGCCCCAAC
    GTGCTGGTAC TCAACACTAA GCATGCTTGG CCCATCTACG TGAGCCCCGG TATCCTGCTG
    CTGTTGTACT ACACAGCCAC CTCTCTCCTG AAGCTCCGCA AGGGCCGCTT CTCAGAGCTG
    AGGAAGGAGA TCCCGAGGGA GGATGAGGAG CATGAACTGG AACTGGACCA GCTGGAGCCA
    GAGCCCCAGG CTAGGGGCAC CACCCAGGGT GCGACGCCCA CGACCACTGG ACCCGACATT
    GACAACTGCA CCGTGCATGT ACTAACCAGC CAGAGCCCCG TCCGCCAGCG TCCTGTACGC
    CCCAGGCTGG CTGAGCTGAA AGAGATGTCA CCACTACATG GCCTGGGCCA CCTCATCCTG
    GACCAGAGCT ATGTGTGTGC TCTGATCGCC ATGATGGTGT GGAGCATCAT GTACCACAGC
    TGGCTGACCT TCGTCTTGCT GCTCTGGGCC TGCCTCATCT GGACCGTGCG CAGCCGCCAC
    CAGTTGGCTA TGCTGTGCTC ACCCTGCATC CTGCTGTACG GACTGACACT CTGCTGCCTG
    CGGTACGTGT GGGCCATGGA GCTCCCCGAG CTGCCCACCA CCCTGGGCCC TGTCAGTCTA
    CATCAGTTGG GATTGGAGCA TACACGCTAC CCCTGCCTGG ACCTTGGTGC CATGCTGCTG
    TATCTGCTCA CGTTCTGGCT GCTCCTGCGT CAGTTCGTGA AGGAGAAGCT GCTGAAGAAG
    AGAAAGGCAC CTTCGACGCT GCTGGAGGTC ACAGTGTCCG ACACAGAGCC CACACAGACC
    CAGACACTGC TGAGGAGCCT GGGGGAGCTG GTCACGGGCA TCTACGTCAA GTACTGGATC
    TACGTGTGCG CTGGGATGTT CATTGTGGTC AGCTTCGCTG GCCGCCTGGT GGTCTACAAA
    ATCGTCTACA TGTTCCTTTT CCTGCTGTGC CTCACGTTGT TCCAGGTCTA CTACACTCTG
    TGGAGGAAGC TGCTCCGAGT TTTCTGGTGG CTCGTGGTGG CCTACACCAT GCTCGTGCTC
    ATCGCCGTGT ACACTTTCCA GTTCCAGGAC TTCCCCACCT ACTGGCGCAA CCTCACGGGC
    TTCACAGATG AACAGTTGGG CGACCTGGGC CTGGAGCAGT TCAGCGTGTC GGAGCTCTTT
    TCCAGCATCC TCATCCCTGG CTTCTTCCTG CTGGCCTGCA TCCTGCAGCT GCACTACTTC
    CACAGGCCGT TCATGCAGCT CACCGACCTG GAGCACGTGC CCCCGCCAGG CACACGCCGC
    CTTCGCTGGG CTCACAGGCA GGATACAGTG AGCGAGGCCC CTCTGCTTCA GCATCAGGAG
    GAGGAGGAAG TCTTCAGGGA TGACGGGCAG AGCATGGATG GGCCCCACCA GACCACACAG
    GTCCCTGAGG GTACAGCCAG CAAGTGGGGC CTGGTGGCCG ACCGGCTGCT GGACCTAGCG
    TCCAGCTTCT CCGCCGTCCT CACCCGCATC CAGGTGTTCG TACGATGTTT GCTCGAGCTT
    CACGTCTTCA AGCTGGTGGC TCTCTACACT GTCTGGGTGG CCCTGAAAGA AGTGTCCGTG
    ATGAACCTGC TGCTGGTGGT GCTGTGGGCC TTCGCCCTGC CCTACCCGCG CTTCCGGCCC
    ATGGCCTCCT GCCTGTCCAC CGTGTGGACC TGTATCATCA TCGTGTGCAA GATGCTGTAT
    CAGCTGAAGA TTGTCAACCC ACACGAGTAC TCCAGTAACT GCACCGAGCC CTTTCCCAAC
    AATACCAACT TGCAGCCCTT GGAGATCAGC CAGTCTCTGC TGTACCGTGG CCCCGTTGAC
    CCTGCCAACT GGTTTGGGGT GCGGAAGGGT TACCCAAACT TGGGCTATAT CCAGAACCAC
    CTGCAGATCC TTCTGCTGCT GGTGTTTGAG GCTGTGGTGT ACCGGCGCCA AGAGCACTAC
    CGCCGGCAGC ACCAGCAGGC CCCTCTGCCC GCCCAGGCTT TGTGCGCTGA TGGCACCCGC
    CAGAGGCTGG ACCAGGACCT ACTCAGCTGC CTCAAATATT TCATCAACTT CTTCTTCTAC
    AAATTCGGGC TGGAGATCTG CTTCTTGATG GCCGTGAATG TCATCGGACA ACGCATGAAC
    TTCATGGTGA TCTTACACGG CTGCTGGTTG GTGGCCATCC TTACACGCCG GCGCCGTGAG
    GCCATCGCCC GCCTCTGGCC CAATTACTGT CTGTTCCTCA CGCTGTTCCT GCTGTACCAG
    TACCTGCTGT GCCTGGGCAT GCCTCCCGCA CTGTGCATCG ACTACCCGTG GCGCTGGAGC
    CAGGCCATCC CCATGAATTC TGCGCTCATC AAGTGGCTGT ACCTCCCTGA CTTCTTCAGG
    GCCCCCAACT CCACCAACCT TATCAGTGAC TTCCTCCTGC TACTCTGCGC CTCCCAACAG
    TGGCAGGTGT TCTCCGCAGA GCAAACGGAA GAATGGCAGC GCATGGCTGG CGTTAACACT
    GACCACCTGG AGCCCCTGCG TGGGGAGCCC AACCCTATCC CCAACTTCAT CCACTGCAGG
    TCCTATCTGG ATATGCTGAA GGTGGCCGTC TTCCGCTACC TGTTCTGGCT GGTGCTGGTG
    GTCGTGTTTG TCACAGGGGC CACCCGCATC AGCATCTTTG GGCTGGGGTA CCTGCTGGCC
    TGCTTCTACC TGCTGCTCTT TGGCACCACC CTGCTGCAGA AGGACACACG AGCCCAGCTC
    GTGCTGTGGG ACTGCCTCAT CCTCTACAAC GTCACCGTCA TCATCTCTAA GAATATGTTG
    TCGCTCCTGT CCTGTGTCTT CGTGGAGCAA ATGCAGAGCA ACTTCTGCTG GGTCATCCAG
    CTCTTCAGCC TCGTGTGCAC AGTCAAAGGC TACTATGACC CCAAAGAGAT GAAGACCAGG
    GACCGGGATT GCCTGCTGCC TGTGGAGGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC
    TTCTTCCTGC TCCTACAACG GCGCGTCTTT CTCAGCCACT ACTTCCTGCA CGTCAGTGCT
    GACCTGAAGG CCACGGCCCT GCAGGCCTCC AGGGGTTTTG CTCTCTACAA TGCGGCCAAC
    ATAAAGAACA TCAACTTCCA CCGTCAGACC GAGGAGAGGT CCCTGGCCCA GCTGAAAAGA
    CAGATGAAGC GCATCCGTGC CAAACAGGAG AAATACCGGC AGAGCCAGGC AAGCCGTGGT
    CAACTCCAGT CCACAGACCC TCAGGAGCCA GTCATCCACT CTGGTGACTA CTTCCTGTTT
    GAGTCGGACA GTGAGGAGGA AGAGGAGGCC CTGCCTGAGG ACCCCAGGCC TGCAGCTCAG
    AGTGCCTTCC AGATGGCATA CCAGGCATGG GTAACCAACG CCCAGACAGT GCTAAGGCAG
    CGTCGTGAAC AGGCACGGCG GGATCGGGCA GAACAGCTGG CTTCTGGAGG CGACTTGAGT
    CCTGAGGTGG AGCCGGTAGA CGTCCCAGAG AATGAGATGG CAGGCCGTAG CCATGTGATG
    CAGCGCGTGC TGAGTACCAT GCAGTTCCTG TGGGTGCTGG GCCAGGCCAC AGTGGACGGG
    CTGACGCGCT GGTTGCGTAC GTTCACGAAG GACCACCGCA CCATGAGTGA TGTGCTGTGT
    GCAGAGCGTT ACCTGCTCAC CCAGGAGCTT CTTCGGGGTG GAGAGGTACA CCGAGGGGTA
    CTGGACCAGC TTTACGTGAG CGAAGATGAG ATCGCACTGT CAGGTCCTGT GGAGAACCGT
    GATGGACCCA GCACAGCCTC TAGCGGGCTG GGAGCTGAAG AGCCTTTGAG TAGCATGACA
    GACGACACCG GCAGCCCCCT TAGCACTGGC TACAACACTC GCAGTGGTAG TGAGGAGATT
    ATCACCGACA CTGGGGGCCT CCAGGCTGGG ACCTCCCTGC ACGGCTCCCA AGAGCTTCTA
    GCCAATGCTC GTACCCGGAT GCGCACAGCC AGTGAGCTGC TGCTGGACAG GCGCCTGCGT
    ATCCCTGAGC TGGAGGAGGC CGAGCAGTTT GAGGCACAGC AGGGCCGGAC GCTGCGGCTG
    CTCAGAGCCA TGTACCAGTG TGTGGCGGCC CACTCAGAGC TTCTCTGTTA CTTCATCATC
    ATCCTTAACC ACATGGTGAC AGCCTCGGCT GCCTCCCTGG TGCTGCCTGT GCTTGTGTTC
    CTCTGGGCCA TGCTGACCAT CCCGAGGCCT AGCAAGCGCT TCTGGATGAC AGCTATCGTC
    TTCACTGAGG TCATGGTGGT CACCAAATAC TTGTTCCAGT TTGGCTTCTT CCCCTGGAAC
    AGCTACATAG TGCTTCGACG CTACGAGAAC AAGCCCTACT TCCCTCCGCG CATCCTGGGC
    CTCGAGAAAA CGGACAGCTA TATCAAGTAC GACCTGGTGC AACTCATGGC CCTCTTCTTC
    CACCGCTCGC AGTTACTGTG CTATGGCCTC TGGGACCATG AGGAGGATGG TGTCCCCAAG
    GACCATTGCA GGAGTAGCGA AAAGGACCAA GAAGCTGAGG AAGAGTCAGA GGCTAAGCTG
    GAATCGCAGC CTGAGACGGG CACTGGGCAT CCTGAGGAGC CCGTGTTGAC TGGTACCCCC
    AAGGACCACA TCCAAGGGAA AGGAAGTGTT AGGTCCAAGG ATGAGATCCA AGATCCCCCA
    GAGGACCTTA AGCCCCAGCA CAGGAGACAC ATCAGCATAC GCTTCAGGAG GAGGAAGGAG
    ACTCAAGGAC CCAAAGGAGC AGCAGTCGTG GAGGCTGAGC ACGAGGAGGG AGAGGAAGGA
    AGAGAAGCTG CAGGGAGAAA GAGGCTGCGT CGCCCTCGAG AAGGGTTGAA GATTCGGGAG
    AAAATGAAGG CAGCTGGACG CCGGCTGCAG AGCTTCTGTC TGTCACTGGC CCAGAGCTTT
    TACCAACCCC TGCGGCGTTT CTTCCATGAC ATTCTGCACA CCAAGTACCG AGCGGCCACC
    GACGTCTATG CCCTCATGTT CCTGGCTGAC ATTGTCGACA TTGTCGTCAT CATCTTCGGT
    TTTTGGGCTT TTGGGAAGCA CTCCGCGGCC ACGGACATTG CATCCTCACT GTCAGATGAC
    CAGGTGCCAC AGGCCTTCCT GTTCATGCTG CTGGTCCAGT TCGGCACCAT GGTCATAGAC
    CGCGCCCTCT ACCTGCGTAA GACTGTACTG GGCAAGCTGG CCTTTCAGGT GGTCCTGGTG
    GTGGCCATTC ACCTCTGGAT GTTTTTCATC TTACCGGCTG TCACCGAGAG GATGTTCAGA
    CAGAACGCGG TGGCCCAGCT GTGGTACTTC GTGAAGTGCA TTTACTTTGC CCTGTCCGCC
    TACCAGATCC GCTGTGGCTA CCCCACCCGT ATCTTGGGCA ACTTCCTCAC CAAGAAGTAC
    AATCATCTAA ACCTCTTCCT TTTCCAGGGG TTCCGTCTAG TGCCATTCCT GGTGGAGCTG
    CGGGCCGTCA TGGACTGGGT ATGGACTGAC ACCACGCTGT CCCTGTCCAA CTGGATGTGT
    GTGGAAGACA TCTATGCCAA CATCTTTATC ATCAAGTGCA GCCGAGAGAC AGAGAAGAAA
    TACCCCCAGC CCAAGGGACA GAAGAAGAAG AAAATCGTCA AGTATGGCAT GGGAGGTCTC
    ATCATCCTCT TCCTCATTGC CATCATCTGG TTCCCTCTGC TCTTCATGTC ACTGATCCGC
    TCTGTGGTTG GTGTCGTCAA CCAGCCCATT GATGTCACTG TTACCCTCAA GTTAGGTGGC
    TACGAGCCAC TGTTCACCAT GAGCGCCCAG CAGCCATCCA TAGTGCCATT CACTCCCGAG
    GACTATGAGG AGCTGTCCCA GCAGTTTGAC CCCTACCCAC TAGCCATGCA GTTCATTAGC
    CAGTACAGTC CTGAGGACAT CGTCACTGCA CAGATTGAGG GCAGCTCGGG GGCCCTGTGG
    CGCATCAGCC CGCCCAGCCG GGCCCAGATG AAGCACGAGC TGTACAACGG CACAGCCGAC
    ATCACACTGC GCTTCACCTG GAATTTCCAA AGGGACCTGG CCAAGGGCGG CTCTGTGGAG
    TATACCAACG AGAAGCACAC CTTGGAGCTG GCCCCCAACA GTACGGCACG AAGGCAGCTG
    GCCCAACTGC TCGAGGGCAG ACCTGACCAG TCAGTGGTCA TTCCCCACCT CTTCCCCAAG
    TACATTCGTG CACCCAATGG GCCTGAAGCC AATCCTGTGA AGCAACTGCA GCCAGACGAG
    GAAGAGGACT ACCTTGGTGT GCGTATCCAG CTGCGGAGAG AGCAAGTGGG CACAGGAACC
    TCTGGGGAGC AAGCAGGCAC AAAGGCCTCC GACTTCCTCG AGTGGTGGGT GATTGAGCTG
    CAGGACTGCC AGGCCGAGTG CAACCTGCTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCGCCTAGCC TGGGCTTCCT GGCCGGCTAC GGGATTGTGG GGCTCTACGT CTCTATCGTG
    CTGGTCGTTG GCAAGTTTGT GCGGGGCTTC TTCAGTGATA TCTCACACTC CATCATGTTC
    GAGGAGCTGC CGTGCGTGGA CCGCATCCTC AAGCTGTGCC AGGACATCTT CTTGGTACGG
    GAGACCCGAG AGCTGGAGCT GGAGGAGGAA CTCTATGCCA AGCTCATATT CCTGTACCGA
    TCACCAGAGA CCATGATCAA GTGGACGCGT GAGAAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008770849.1
    CDS618..8159
    Translation

    Target ORF information:

    RefSeq Version XM_008772627.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008772627.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
    GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG
    CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT
    CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC
    CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGCAGCAG CCTTTGGGCA
    AACGTGTCTC AACACATAGG GGTTACAAGG CTGGACCTGA AGGACATCTT TAACACCACC
    AGACTGGTGG CACCTGACCT GGGAGTGCTG GTGGCATCTT CCCTTTGCCT TGGCCTCTGT
    GGACGCCTCA CAAGGAAAGC CCGGCAGAGC CAGCGCACTC AGGAGCTGCA GGAGGAGGAT
    GACATAGATG CTGCCCCCGC CGCAGGGCTG CAGGGAGCCC CCACCCTGGC AACCAAACGC
    AGGCTGTGGC TGGCCCCTCG CTTCCGGATC ACGGCGCACT GGCTGCTGGT GACCTCCGGA
    CGGATGCTGG TCATCGTGCT ACTGGCACTC GCAGGCATAG CCCATCCTTC GGCCTTCTCC
    AGCGTCTACC TGATGGTGTT CCTGGCCATC TGCACCTGGT GGTCTTGCCA CTTTCCTCTC
    AGCTCCCTGG GCTTTAACAC CCTCTGCGTC ATGGTGAGCT GCTTTGGCGC CGGCCATCTT
    GTTTGTTTAT ACTGCTATCA GACACCATTT GTCCAGAGCG TGCTACTTCC TGGCAGCCTC
    TGGGCCAGGC TATTTGGTCT CAAGAACTTC GTAGACATCC CTAACTGCTC CAGCCCCAAC
    GTGCTGGTAC TCAACACTAA GCATGCTTGG CCCATCTACG TGAGCCCCGG TATCCTGCTG
    CTGTTGTACT ACACAGCCAC CTCTCTCCTG AAGCTCCGCA AGGGCCGCTT CTCAGAGCTG
    AGGAAGGAGA TCCCGAGGGA GGATGAGGAG CATGAACTGG AACTGGACCA GCTGGAGCCA
    GAGCCCCAGG CTAGGGGCAC CACCCAGGGT GCGACGCCCA CGACCACTGG ACCCGACATT
    GACAACTGCA CCGTGCATGT ACTAACCAGC CAGAGCCCCG TCCGCCAGCG TCCTGTACGC
    CCCAGGCTGG CTGAGCTGAA AGAGATGTCA CCACTACATG GCCTGGGCCA CCTCATCCTG
    GACCAGAGCT ATGTGTGTGC TCTGATCGCC ATGATGGTGT GGAGCATCAT GTACCACAGC
    TGGCTGACCT TCGTCTTGCT GCTCTGGGCC TGCCTCATCT GGACCGTGCG CAGCCGCCAC
    CAGTTGGCTA TGCTGTGCTC ACCCTGCATC CTGCTGTACG GACTGACACT CTGCTGCCTG
    CGGTACGTGT GGGCCATGGA GCTCCCCGAG CTGCCCACCA CCCTGGGCCC TGTCAGTCTA
    CATCAGTTGG GATTGGAGCA TACACGCTAC CCCTGCCTGG ACCTTGGTGC CATGCTGCTG
    TATCTGCTCA CGTTCTGGCT GCTCCTGCGT CAGTTCGTGA AGGAGAAGCT GCTGAAGAAG
    AGAAAGGCAC CTTCGACGCT GCTGGAGGTC ACAGTGTCCG ACACAGAGCC CACACAGACC
    CAGACACTGC TGAGGAGCCT GGGGGAGCTG GTCACGGGCA TCTACGTCAA GTACTGGATC
    TACGTGTGCG CTGGGATGTT CATTGTGGTC AGCTTCGCTG GCCGCCTGGT GGTCTACAAA
    ATCGTCTACA TGTTCCTTTT CCTGCTGTGC CTCACGTTGT TCCAGGTCTA CTACACTCTG
    TGGAGGAAGC TGCTCCGAGT TTTCTGGTGG CTCGTGGTGG CCTACACCAT GCTCGTGCTC
    ATCGCCGTGT ACACTTTCCA GTTCCAGGAC TTCCCCACCT ACTGGCGCAA CCTCACGGGC
    TTCACAGATG AACAGTTGGG CGACCTGGGC CTGGAGCAGT TCAGCGTGTC GGAGCTCTTT
    TCCAGCATCC TCATCCCTGG CTTCTTCCTG CTGGCCTGCA TCCTGCAGCT GCACTACTTC
    CACAGGCCGT TCATGCAGCT CACCGACCTG GAGCACGTGC CCCCGCCAGG CACACGCCGC
    CTTCGCTGGG CTCACAGGCA GGATACAGTG AGCGAGGCCC CTCTGCTTCA GCATCAGGAG
    GAGGAGGAAG TCTTCAGGGA TGACGGGCAG AGCATGGATG GGCCCCACCA GACCACACAG
    GTCCCTGAGG GTACAGCCAG CAAGTGGGGC CTGGTGGCCG ACCGGCTGCT GGACCTAGCG
    TCCAGCTTCT CCGCCGTCCT CACCCGCATC CAGGTGTTCG TACGATGTTT GCTCGAGCTT
    CACGTCTTCA AGCTGGTGGC TCTCTACACT GTCTGGGTGG CCCTGAAAGA AGTGTCCGTG
    ATGAACCTGC TGCTGGTGGT GCTGTGGGCC TTCGCCCTGC CCTACCCGCG CTTCCGGCCC
    ATGGCCTCCT GCCTGTCCAC CGTGTGGACC TGTATCATCA TCGTGTGCAA GATGCTGTAT
    CAGCTGAAGA TTGTCAACCC ACACGAGTAC TCCAGTAACT GCACCGAGCC CTTTCCCAAC
    AATACCAACT TGCAGCCCTT GGAGATCAGC CAGTCTCTGC TGTACCGTGG CCCCGTTGAC
    CCTGCCAACT GGTTTGGGGT GCGGAAGGGT TACCCAAACT TGGGCTATAT CCAGAACCAC
    CTGCAGATCC TTCTGCTGCT GGTGTTTGAG GCTGTGGTGT ACCGGCGCCA AGAGCACTAC
    CGCCGGCAGC ACCAGCAGGC CCCTCTGCCC GCCCAGGCTT TGTGCGCTGA TGGCACCCGC
    CAGAGGCTGG ACCAGGACCT ACTCAGCTGC CTCAAATATT TCATCAACTT CTTCTTCTAC
    AAATTCGGGC TGGAGATCTG CTTCTTGATG GCCGTGAATG TCATCGGACA ACGCATGAAC
    TTCATGGTGA TCTTACACGG CTGCTGGTTG GTGGCCATCC TTACACGCCG GCGCCGTGAG
    GCCATCGCCC GCCTCTGGCC CAATTACTGT CTGTTCCTCA CGCTGTTCCT GCTGTACCAG
    TACCTGCTGT GCCTGGGCAT GCCTCCCGCA CTGTGCATCG ACTACCCGTG GCGCTGGAGC
    CAGGCCATCC CCATGAATTC TGCGCTCATC AAGTGGCTGT ACCTCCCTGA CTTCTTCAGG
    GCCCCCAACT CCACCAACCT TATCAGTGAC TTCCTCCTGC TACTCTGCGC CTCCCAACAG
    TGGCAGGTGT TCTCCGCAGA GCAAACGGAA GAATGGCAGC GCATGGCTGG CGTTAACACT
    GACCACCTGG AGCCCCTGCG TGGGGAGCCC AACCCTATCC CCAACTTCAT CCACTGCAGG
    TCCTATCTGG ATATGCTGAA GGTGGCCGTC TTCCGCTACC TGTTCTGGCT GGTGCTGGTG
    GTCGTGTTTG TCACAGGGGC CACCCGCATC AGCATCTTTG GGCTGGGGTA CCTGCTGGCC
    TGCTTCTACC TGCTGCTCTT TGGCACCACC CTGCTGCAGA AGGACACACG AGCCCAGCTC
    GTGCTGTGGG ACTGCCTCAT CCTCTACAAC GTCACCGTCA TCATCTCTAA GAATATGTTG
    TCGCTCCTGT CCTGTGTCTT CGTGGAGCAA ATGCAGAGCA ACTTCTGCTG GGTCATCCAG
    CTCTTCAGCC TCGTGTGCAC AGTCAAAGGC TACTATGACC CCAAAGAGAT GAAGACCAGG
    GACCGGGATT GCCTGCTGCC TGTGGAGGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC
    TTCTTCCTGC TCCTACAACG GCGCGTCTTT CTCAGCCACT ACTTCCTGCA CGTCAGTGCT
    GACCTGAAGG CCACGGCCCT GCAGGCCTCC AGGGGTTTTG CTCTCTACAA TGCGGCCAAC
    ATAAAGAACA TCAACTTCCA CCGTCAGACC GAGGAGAGGT CCCTGGCCCA GCTGAAAAGA
    CAGATGAAGC GCATCCGTGC CAAACAGGAG AAATACCGGC AGAGCCAGGC AAGCCGTGGT
    CAACTCCAGT CCACAGACCC TCAGGAGCCA GTCATCCACT CTGGTGACTA CTTCCTGTTT
    GAGTCGGACA GTGAGGAGGA AGAGGAGGCC CTGCCTGAGG ACCCCAGGCC TGCAGCTCAG
    AGTGCCTTCC AGATGGCATA CCAGGCATGG GTAACCAACG CCCAGACAGT GCTAAGGCAG
    CGTCGTGAAC AGGCACGGCG GGATCGGGCA GAACAGCTGG CTTCTGGAGG CGACTTGAGT
    CCTGAGGTGG AGCCGGTAGA CGTCCCAGAG AATGAGATGG CAGGCCGTAG CCATGTGATG
    CAGCGCGTGC TGAGTACCAT GCAGTTCCTG TGGGTGCTGG GCCAGGCCAC AGTGGACGGG
    CTGACGCGCT GGTTGCGTAC GTTCACGAAG GACCACCGCA CCATGAGTGA TGTGCTGTGT
    GCAGAGCGTT ACCTGCTCAC CCAGGAGCTT CTTCGGGGTG GAGAGGTACA CCGAGGGGTA
    CTGGACCAGC TTTACGTGAG CGAAGATGAG ATCGCACTGT CAGGTCCTGT GGAGAACCGT
    GATGGACCCA GCACAGCCTC TAGCGGGCTG GGAGCTGAAG AGCCTTTGAG TAGCATGACA
    GACGACACCG GCAGCCCCCT TAGCACTGGC TACAACACTC GCAGTGGTAG TGAGGAGATT
    ATCACCGACA CTGGGGGCCT CCAGGCTGGG ACCTCCCTGC ACGGCTCCCA AGAGCTTCTA
    GCCAATGCTC GTACCCGGAT GCGCACAGCC AGTGAGCTGC TGCTGGACAG GCGCCTGCGT
    ATCCCTGAGC TGGAGGAGGC CGAGCAGTTT GAGGCACAGC AGGGCCGGAC GCTGCGGCTG
    CTCAGAGCCA TGTACCAGTG TGTGGCGGCC CACTCAGAGC TTCTCTGTTA CTTCATCATC
    ATCCTTAACC ACATGGTGAC AGCCTCGGCT GCCTCCCTGG TGCTGCCTGT GCTTGTGTTC
    CTCTGGGCCA TGCTGACCAT CCCGAGGCCT AGCAAGCGCT TCTGGATGAC AGCTATCGTC
    TTCACTGAGG TCATGGTGGT CACCAAATAC TTGTTCCAGT TTGGCTTCTT CCCCTGGAAC
    AGCTACATAG TGCTTCGACG CTACGAGAAC AAGCCCTACT TCCCTCCGCG CATCCTGGGC
    CTCGAGAAAA CGGACAGCTA TATCAAGTAC GACCTGGTGC AACTCATGGC CCTCTTCTTC
    CACCGCTCGC AGTTACTGTG CTATGGCCTC TGGGACCATG AGGAGGATGG TGTCCCCAAG
    GACCATTGCA GGAGTAGCGA AAAGGACCAA GAAGCTGAGG AAGAGTCAGA GGCTAAGCTG
    GAATCGCAGC CTGAGACGGG CACTGGGCAT CCTGAGGAGC CCGTGTTGAC TGGTACCCCC
    AAGGACCACA TCCAAGGGAA AGGAAGTGTT AGGTCCAAGG ATGAGATCCA AGATCCCCCA
    GAGGACCTTA AGCCCCAGCA CAGGAGACAC ATCAGCATAC GCTTCAGGAG GAGGAAGGAG
    ACTCAAGGAC CCAAAGGAGC AGCAGTCGTG GAGGCTGAGC ACGAGGAGGG AGAGGAAGGA
    AGAGAAGCTG CAGGGAGAAA GAGGCTGCGT CGCCCTCGAG AAGGGTTGAA GATTCGGGAG
    AAAATGAAGG CAGCTGGACG CCGGCTGCAG AGCTTCTGTC TGTCACTGGC CCAGAGCTTT
    TACCAACCCC TGCGGCGTTT CTTCCATGAC ATTCTGCACA CCAAGTACCG AGCGGCCACC
    GACGTCTATG CCCTCATGTT CCTGGCTGAC ATTGTCGACA TTGTCGTCAT CATCTTCGGT
    TTTTGGGCTT TTGGGAAGCA CTCCGCGGCC ACGGACATTG CATCCTCACT GTCAGATGAC
    CAGGTGCCAC AGGCCTTCCT GTTCATGCTG CTGGTCCAGT TCGGCACCAT GGTCATAGAC
    CGCGCCCTCT ACCTGCGTAA GACTGTACTG GGCAAGCTGG CCTTTCAGGT GGTCCTGGTG
    GTGGCCATTC ACCTCTGGAT GTTTTTCATC TTACCGGCTG TCACCGAGAG GATGTTCAGA
    CAGAACGCGG TGGCCCAGCT GTGGTACTTC GTGAAGTGCA TTTACTTTGC CCTGTCCGCC
    TACCAGATCC GCTGTGGCTA CCCCACCCGT ATCTTGGGCA ACTTCCTCAC CAAGAAGTAC
    AATCATCTAA ACCTCTTCCT TTTCCAGGGG TTCCGTCTAG TGCCATTCCT GGTGGAGCTG
    CGGGCCGTCA TGGACTGGGT ATGGACTGAC ACCACGCTGT CCCTGTCCAA CTGGATGTGT
    GTGGAAGACA TCTATGCCAA CATCTTTATC ATCAAGTGCA GCCGAGAGAC AGAGAAGAAA
    TACCCCCAGC CCAAGGGACA GAAGAAGAAG AAAATCGTCA AGTATGGCAT GGGAGGTCTC
    ATCATCCTCT TCCTCATTGC CATCATCTGG TTCCCTCTGC TCTTCATGTC ACTGATCCGC
    TCTGTGGTTG GTGTCGTCAA CCAGCCCATT GATGTCACTG TTACCCTCAA GTTAGGTGGC
    TACGAGCCAC TGTTCACCAT GAGCGCCCAG CAGCCATCCA TAGTGCCATT CACTCCCGAG
    GACTATGAGG AGCTGTCCCA GCAGTTTGAC CCCTACCCAC TAGCCATGCA GTTCATTAGC
    CAGTACAGTC CTGAGGACAT CGTCACTGCA CAGATTGAGG GCAGCTCGGG GGCCCTGTGG
    CGCATCAGCC CGCCCAGCCG GGCCCAGATG AAGCACGAGC TGTACAACGG CACAGCCGAC
    ATCACACTGC GCTTCACCTG GAATTTCCAA AGGGACCTGG CCAAGGGCGG CTCTGTGGAG
    TATACCAACG AGAAGCACAC CTTGGAGCTG GCCCCCAACA GTACGGCACG AAGGCAGCTG
    GCCCAACTGC TCGAGGGCAG ACCTGACCAG TCAGTGGTCA TTCCCCACCT CTTCCCCAAG
    TACATTCGTG CACCCAATGG GCCTGAAGCC AATCCTGTGA AGCAACTGCA GCCAGACGAG
    GAAGAGGACT ACCTTGGTGT GCGTATCCAG CTGCGGAGAG AGCAAGTGGG CACAGGAACC
    TCTGGGGAGC AAGCAGGCAC AAAGGCCTCC GACTTCCTCG AGTGGTGGGT GATTGAGCTG
    CAGGACTGCC AGGCCGAGTG CAACCTGCTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCGCCTAGCC TGGGCTTCCT GGCCGGCTAC GGGATTGTGG GGCTCTACGT CTCTATCGTG
    CTGGTCGTTG GCAAGTTTGT GCGGGGCTTC TTCAGTGATA TCTCACACTC CATCATGTTC
    GAGGAGCTGC CGTGCGTGGA CCGCATCCTC AAGCTGTGCC AGGACATCTT CTTGGTACGG
    GAGACCCGAG AGCTGGAGCT GGAGGAGGAA CTCTATGCCA AGCTCATATT CCTGTACCGA
    TCACCAGAGA CCATGATCAA GTGGACGCGT GAGAAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa47914
    Clone ID Related Accession (Same CDS sequence) XM_017601325.1
    Accession Version XM_017601325.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product piezo-type mechanosensitive ion channel component 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCCTCGCC TGAACCAGCT TCTGGGACAA AACTGCAGCA GCCTTTGGGC AAACGTGTCT 
    CAACACATAG GGGTTACAAG GCTGGACCTG AAGGACATCT TTAACACCAC CAGACTGGTG
    GCACCTGACC TGGGAGTGCT GGTGGCATCT TCCCTTTGCC TTGGCCTCTG TGGACGCCTC
    ACAAGGAAAG CCCGGCAGAG CCAGCGCACT CAGGAGCTGC AGGAGGAGGA TGACATAGAT
    GCTGCCCCCG CCGCAGGGCT GCAGGGAGCC CCCACCCTGG CAACCAAACG CAGGCTGTGG
    CTGGCCCCTC GCTTCCGGAT CACGGCGCAC TGGCTGCTGG TGACCTCCGG ACGGATGCTG
    GTCATCGTGC TACTGGCACT CGCAGGCATA GCCCATCCTT CGGCCTTCTC CAGCGTCTAC
    CTGATGGTGT TCCTGGCCAT CTGCACCTGG TGGTCTTGCC ACTTTCCTCT CAGCTCCCTG
    GGCTTTAACA CCCTCTGCGT CATGGTGAGC TGCTTTGGCG CCGGCCATCT TGTTTGTTTA
    TACTGCTATC AGACACCATT TGTCCAGAGC GTGCTACTTC CTGGCAGCCT CTGGGCCAGG
    CTATTTGGTC TCAAGAACTT CGTAGACATC CCTAACTGCT CCAGCCCCAA CGTGCTGGTA
    CTCAACACTA AGCATGCTTG GCCCATCTAC GTGAGCCCCG GTATCCTGCT GCTGTTGTAC
    TACACAGCCA CCTCTCTCCT GAAGCTCCGC AAGGGCCGCT TCTCAGAGCT GAGGAAGGAG
    ATCCCGAGGG AGGATGAGGA GCATGAACTG GAACTGGACC AGCTGGAGCC AGAGCCCCAG
    GCTAGGGGCA CCACCCAGGG TGCGACGCCC ACGACCACTG GACCCGACAT TGACAACTGC
    ACCGTGCATG TACTAACCAG CCAGAGCCCC GTCCGCCAGC GTCCTGTACG CCCCAGGCTG
    GCTGAGCTGA AAGAGATGTC ACCACTACAT GGCCTGGGCC ACCTCATCCT GGACCAGAGC
    TATGTGTGTG CTCTGATCGC CATGATGGTG TGGAGCATCA TGTACCACAG CTGGCTGACC
    TTCGTCTTGC TGCTCTGGGC CTGCCTCATC TGGACCGTGC GCAGCCGCCA CCAGTTGGCT
    ATGCTGTGCT CACCCTGCAT CCTGCTGTAC GGACTGACAC TCTGCTGCCT GCGGTACGTG
    TGGGCCATGG AGCTCCCCGA GCTGCCCACC ACCCTGGGCC CTGTCAGTCT ACATCAGTTG
    GGATTGGAGC ATACACGCTA CCCCTGCCTG GACCTTGGTG CCATGCTGCT GTATCTGCTC
    ACGTTCTGGC TGCTCCTGCG TCAGTTCGTG AAGGAGAAGC TGCTGAAGAA GAGAAAGGCA
    CCTTCGACGC TGCTGGAGGT CACAGTGTCC GACACAGAGC CCACACAGAC CCAGACACTG
    CTGAGGAGCC TGGGGGAGCT GGTCACGGGC ATCTACGTCA AGTACTGGAT CTACGTGTGC
    GCTGGGATGT TCATTGTGGT CAGCTTCGCT GGCCGCCTGG TGGTCTACAA AATCGTCTAC
    ATGTTCCTTT TCCTGCTGTG CCTCACGTTG TTCCAGGTCT ACTACACTCT GTGGAGGAAG
    CTGCTCCGAG TTTTCTGGTG GCTCGTGGTG GCCTACACCA TGCTCGTGCT CATCGCCGTG
    TACACTTTCC AGTTCCAGGA CTTCCCCACC TACTGGCGCA ACCTCACGGG CTTCACAGAT
    GAACAGTTGG GCGACCTGGG CCTGGAGCAG TTCAGCGTGT CGGAGCTCTT TTCCAGCATC
    CTCATCCCTG GCTTCTTCCT GCTGGCCTGC ATCCTGCAGC TGCACTACTT CCACAGGCCG
    TTCATGCAGC TCACCGACCT GGAGCACGTG CCCCCGCCAG GCACACGCCG CCTTCGCTGG
    GCTCACAGGC AGGATACAGT GAGCGAGGCC CCTCTGCTTC AGCATCAGGA GGAGGAGGAA
    GTCTTCAGGG ATGACGGGCA GAGCATGGAT GGGCCCCACC AGACCACACA GGTCCCTGAG
    GGTACAGCCA GCAAGTGGGG CCTGGTGGCC GACCGGCTGC TGGACCTAGC GTCCAGCTTC
    TCCGCCGTCC TCACCCGCAT CCAGGTGTTC GTACGATGTT TGCTCGAGCT TCACGTCTTC
    AAGCTGGTGG CTCTCTACAC TGTCTGGGTG GCCCTGAAAG AAGTGTCCGT GATGAACCTG
    CTGCTGGTGG TGCTGTGGGC CTTCGCCCTG CCCTACCCGC GCTTCCGGCC CATGGCCTCC
    TGCCTGTCCA CCGTGTGGAC CTGTATCATC ATCGTGTGCA AGATGCTGTA TCAGCTGAAG
    ATTGTCAACC CACACGAGTA CTCCAGTAAC TGCACCGAGC CCTTTCCCAA CAATACCAAC
    TTGCAGCCCT TGGAGATCAG CCAGTCTCTG CTGTACCGTG GCCCCGTTGA CCCTGCCAAC
    TGGTTTGGGG TGCGGAAGGG TTACCCAAAC TTGGGCTATA TCCAGAACCA CCTGCAGATC
    CTTCTGCTGC TGGTGTTTGA GGCTGTGGTG TACCGGCGCC AAGAGCACTA CCGCCGGCAG
    CACCAGCAGG CCCCTCTGCC CGCCCAGGCT TTGTGCGCTG ATGGCACCCG CCAGAGGCTG
    GACCAGGACC TACTCAGCTG CCTCAAATAT TTCATCAACT TCTTCTTCTA CAAATTCGGG
    CTGGAGATCT GCTTCTTGAT GGCCGTGAAT GTCATCGGAC AACGCATGAA CTTCATGGTG
    ATCTTACACG GCTGCTGGTT GGTGGCCATC CTTACACGCC GGCGCCGTGA GGCCATCGCC
    CGCCTCTGGC CCAATTACTG TCTGTTCCTC ACGCTGTTCC TGCTGTACCA GTACCTGCTG
    TGCCTGGGCA TGCCTCCCGC ACTGTGCATC GACTACCCGT GGCGCTGGAG CCAGGCCATC
    CCCATGAATT CTGCGCTCAT CAAGTGGCTG TACCTCCCTG ACTTCTTCAG GGCCCCCAAC
    TCCACCAACC TTATCAGTGA CTTCCTCCTG CTACTCTGCG CCTCCCAACA GTGGCAGGTG
    TTCTCCGCAG AGCAAACGGA AGAATGGCAG CGCATGGCTG GCGTTAACAC TGACCACCTG
    GAGCCCCTGC GTGGGGAGCC CAACCCTATC CCCAACTTCA TCCACTGCAG GTCCTATCTG
    GATATGCTGA AGGTGGCCGT CTTCCGCTAC CTGTTCTGGC TGGTGCTGGT GGTCGTGTTT
    GTCACAGGGG CCACCCGCAT CAGCATCTTT GGGCTGGGGT ACCTGCTGGC CTGCTTCTAC
    CTGCTGCTCT TTGGCACCAC CCTGCTGCAG AAGGACACAC GAGCCCAGCT CGTGCTGTGG
    GACTGCCTCA TCCTCTACAA CGTCACCGTC ATCATCTCTA AGAATATGTT GTCGCTCCTG
    TCCTGTGTCT TCGTGGAGCA AATGCAGAGC AACTTCTGCT GGGTCATCCA GCTCTTCAGC
    CTCGTGTGCA CAGTCAAAGG CTACTATGAC CCCAAAGAGA TGAAGACCAG GGACCGGGAT
    TGCCTGCTGC CTGTGGAGGA GGCCGGGATC ATCTGGGACA GCATCTGCTT CTTCTTCCTG
    CTCCTACAAC GGCGCGTCTT TCTCAGCCAC TACTTCCTGC ACGTCAGTGC TGACCTGAAG
    GCCACGGCCC TGCAGGCCTC CAGGGGTTTT GCTCTCTACA ATGCGGCCAA CATAAAGAAC
    ATCAACTTCC ACCGTCAGAC CGAGGAGAGG TCCCTGGCCC AGCTGAAAAG ACAGATGAAG
    CGCATCCGTG CCAAACAGGA GAAATACCGG CAGAGCCAGG CAAGCCGTGG TCAACTCCAG
    TCCACAGACC CTCAGGAGCC AGGGCCTGAC AGCCCAGGGG GTTCCTCCCC GCCACGGACA
    CAGTGGTGGC GCCCCTGGCA GGACCACGCC ACAGTCATCC ACTCTGGTGA CTACTTCCTG
    TTTGAGTCGG ACAGTGAGGA GGAAGAGGAG GCCCTGCCTG AGGACCCCAG GCCTGCAGCT
    CAGAGTGCCT TCCAGATGGC ATACCAGGCA TGGGTAACCA ACGCCCAGAC AGTGCTAAGG
    CAGCGTCGTG AACAGGCACG GCGGGATCGG GCAGAACAGC TGGCTTCTGG AGGCGACTTG
    AGTCCTGAGG TGGAGCCGGT AGACGTCCCA GAGAATGAGA TGGCAGGCCG TAGCCATGTG
    ATGCAGCGCG TGCTGAGTAC CATGCAGTTC CTGTGGGTGC TGGGCCAGGC CACAGTGGAC
    GGGCTGACGC GCTGGTTGCG TACGTTCACG AAGGACCACC GCACCATGAG TGATGTGCTG
    TGTGCAGAGC GTTACCTGCT CACCCAGGAG CTTCTTCGGG GTGGAGAGGT ACACCGAGGG
    GTACTGGACC AGCTTTACGT GAGCGAAGAT GAGATCGCAC TGTCAGGTCC TGTGGAGAAC
    CGTGATGGAC CCAGCACAGC CTCTAGCGGG CTGGGAGCTG AAGAGCCTTT GAGTAGCATG
    ACAGACGACA CCGGCAGCCC CCTTAGCACT GGCTACAACA CTCGCAGTGG TAGTGAGGAG
    ATTATCACCG ACACTGGGGG CCTCCAGGCT GGGACCTCCC TGCACGGCTC CCAAGAGCTT
    CTAGCCAATG CTCGTACCCG GATGCGCACA GCCAGTGAGC TGCTGCTGGA CAGGCGCCTG
    CGTATCCCTG AGCTGGAGGA GGCCGAGCAG TTTGAGGCAC AGCAGGGCCG GACGCTGCGG
    CTGCTCAGAG CCATGTACCA GTGTGTGGCG GCCCACTCAG AGCTTCTCTG TTACTTCATC
    ATCATCCTTA ACCACATGGT GACAGCCTCG GCTGCCTCCC TGGTGCTGCC TGTGCTTGTG
    TTCCTCTGGG CCATGCTGAC CATCCCGAGG CCTAGCAAGC GCTTCTGGAT GACAGCTATC
    GTCTTCACTG AGGTCATGGT GGTCACCAAA TACTTGTTCC AGTTTGGCTT CTTCCCCTGG
    AACAGCTACA TAGTGCTTCG ACGCTACGAG AACAAGCCCT ACTTCCCTCC GCGCATCCTG
    GGCCTCGAGA AAACGGACAG CTATATCAAG TACGACCTGG TGCAACTCAT GGCCCTCTTC
    TTCCACCGCT CGCAGTTACT GTGCTATGGC CTCTGGGACC ATGAGGAGGA TGGTGTCCCC
    AAGGACCATT GCAGGAGTAG CGAAAAGGAC CAAGAAGCTG AGGAAGAGTC AGAGGCTAAG
    CTGGAATCGC AGCCTGAGAC GGGCACTGGG CATCCTGAGG AGCCCGTGTT GACTGGTACC
    CCCAAGGACC ACATCCAAGG GAAAGGAAGT GTTAGGTCCA AGGATGAGAT CCAAGATCCC
    CCAGAGGACC TTAAGCCCCA GCACAGGAGA CACATCAGCA TACGCTTCAG GAGGAGGAAG
    GAGACTCAAG GACCCAAAGG AGCAGCAGTC GTGGAGGCTG AGCACGAGGA GGGAGAGGAA
    GGAAGAGAAG CTGCAGGGAG AAAGAGGCTG CGTCGCCCTC GAGAAGGGTT GAAGATTCGG
    GAGAAAATGA AGGCAGCTGG ACGCCGGCTG CAGAGCTTCT GTCTGTCACT GGCCCAGAGC
    TTTTACCAAC CCCTGCGGCG TTTCTTCCAT GACATTCTGC ACACCAAGTA CCGAGCGGCC
    ACCGACGTCT ATGCCCTCAT GTTCCTGGCT GACATTGTCG ACATTGTCGT CATCATCTTC
    GGTTTTTGGG CTTTTGGGAA GCACTCCGCG GCCACGGACA TTGCATCCTC ACTGTCAGAT
    GACCAGGTGC CACAGGCCTT CCTGTTCATG CTGCTGGTCC AGTTCGGCAC CATGGTCATA
    GACCGCGCCC TCTACCTGCG TAAGACTGTA CTGGGCAAGC TGGCCTTTCA GGTGGTCCTG
    GTGGTGGCCA TTCACCTCTG GATGTTTTTC ATCTTACCGG CTGTCACCGA GAGGATGTTC
    AGACAGAACG CGGTGGCCCA GCTGTGGTAC TTCGTGAAGT GCATTTACTT TGCCCTGTCC
    GCCTACCAGA TCCGCTGTGG CTACCCCACC CGTATCTTGG GCAACTTCCT CACCAAGAAG
    TACAATCATC TAAACCTCTT CCTTTTCCAG GGGTTCCGTC TAGTGCCATT CCTGGTGGAG
    CTGCGGGCCG TCATGGACTG GGTATGGACT GACACCACGC TGTCCCTGTC CAACTGGATG
    TGTGTGGAAG ACATCTATGC CAACATCTTT ATCATCAAGT GCAGCCGAGA GACAGAGAAG
    AAATACCCCC AGCCCAAGGG ACAGAAGAAG AAGAAAATCG TCAAGTATGG CATGGGAGGT
    CTCATCATCC TCTTCCTCAT TGCCATCATC TGGTTCCCTC TGCTCTTCAT GTCACTGATC
    CGCTCTGTGG TTGGTGTCGT CAACCAGCCC ATTGATGTCA CTGTTACCCT CAAGTTAGGT
    GGCTACGAGC CACTGTTCAC CATGAGCGCC CAGCAGCCAT CCATAGTGCC ATTCACTCCC
    GAGGACTATG AGGAGCTGTC CCAGCAGTTT GACCCCTACC CACTAGCCAT GCAGTTCATT
    AGCCAGTACA GTCCTGAGGA CATCGTCACT GCACAGATTG AGGGCAGCTC GGGGGCCCTG
    TGGCGCATCA GCCCGCCCAG CCGGGCCCAG ATGAAGCACG AGCTGTACAA CGGCACAGCC
    GACATCACAC TGCGCTTCAC CTGGAATTTC CAAAGGGACC TGGCCAAGGG CGGCTCTGTG
    GAGTATACCA ACGAGAAGCA CACCTTGGAG CTGGCCCCCA ACAGTACGGC ACGAAGGCAG
    CTGGCCCAAC TGCTCGAGGG CAGACCTGAC CAGTCAGTGG TCATTCCCCA CCTCTTCCCC
    AAGTACATTC GTGCACCCAA TGGGCCTGAA GCCAATCCTG TGAAGCAACT GCAGCCAGAC
    GAGGAAGAGG ACTACCTTGG TGTGCGTATC CAGCTGCGGA GAGAGCAAGT GGGCACAGGA
    ACCTCTGGGG AGCAAGCAGG CACAAAGGCC TCCGACTTCC TCGAGTGGTG GGTGATTGAG
    CTGCAGGACT GCCAGGCCGA GTGCAACCTG CTGCCCATGG TCATCTTCAG TGACAAGGTC
    AGCCCGCCTA GCCTGGGCTT CCTGGCCGGC TACGGGATTG TGGGGCTCTA CGTCTCTATC
    GTGCTGGTCG TTGGCAAGTT TGTGCGGGGC TTCTTCAGTG ATATCTCACA CTCCATCATG
    TTCGAGGAGC TGCCGTGCGT GGACCGCATC CTCAAGCTGT GCCAGGACAT CTTCTTGGTA
    CGGGAGACCC GAGAGCTGGA GCTGGAGGAG GAACTCTATG CCAAGCTCAT ATTCCTGTAC
    CGATCACCAG AGACCATGAT CAAGTGGACG CGTGAGAAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017456814.1
    CDS6322..13686
    Translation

    Target ORF information:

    RefSeq Version XM_017601325.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017601325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCCTCGCC TGAACCAGCT TCTGGGACAA AACTGCAGCA GCCTTTGGGC AAACGTGTCT 
    CAACACATAG GGGTTACAAG GCTGGACCTG AAGGACATCT TTAACACCAC CAGACTGGTG
    GCACCTGACC TGGGAGTGCT GGTGGCATCT TCCCTTTGCC TTGGCCTCTG TGGACGCCTC
    ACAAGGAAAG CCCGGCAGAG CCAGCGCACT CAGGAGCTGC AGGAGGAGGA TGACATAGAT
    GCTGCCCCCG CCGCAGGGCT GCAGGGAGCC CCCACCCTGG CAACCAAACG CAGGCTGTGG
    CTGGCCCCTC GCTTCCGGAT CACGGCGCAC TGGCTGCTGG TGACCTCCGG ACGGATGCTG
    GTCATCGTGC TACTGGCACT CGCAGGCATA GCCCATCCTT CGGCCTTCTC CAGCGTCTAC
    CTGATGGTGT TCCTGGCCAT CTGCACCTGG TGGTCTTGCC ACTTTCCTCT CAGCTCCCTG
    GGCTTTAACA CCCTCTGCGT CATGGTGAGC TGCTTTGGCG CCGGCCATCT TGTTTGTTTA
    TACTGCTATC AGACACCATT TGTCCAGAGC GTGCTACTTC CTGGCAGCCT CTGGGCCAGG
    CTATTTGGTC TCAAGAACTT CGTAGACATC CCTAACTGCT CCAGCCCCAA CGTGCTGGTA
    CTCAACACTA AGCATGCTTG GCCCATCTAC GTGAGCCCCG GTATCCTGCT GCTGTTGTAC
    TACACAGCCA CCTCTCTCCT GAAGCTCCGC AAGGGCCGCT TCTCAGAGCT GAGGAAGGAG
    ATCCCGAGGG AGGATGAGGA GCATGAACTG GAACTGGACC AGCTGGAGCC AGAGCCCCAG
    GCTAGGGGCA CCACCCAGGG TGCGACGCCC ACGACCACTG GACCCGACAT TGACAACTGC
    ACCGTGCATG TACTAACCAG CCAGAGCCCC GTCCGCCAGC GTCCTGTACG CCCCAGGCTG
    GCTGAGCTGA AAGAGATGTC ACCACTACAT GGCCTGGGCC ACCTCATCCT GGACCAGAGC
    TATGTGTGTG CTCTGATCGC CATGATGGTG TGGAGCATCA TGTACCACAG CTGGCTGACC
    TTCGTCTTGC TGCTCTGGGC CTGCCTCATC TGGACCGTGC GCAGCCGCCA CCAGTTGGCT
    ATGCTGTGCT CACCCTGCAT CCTGCTGTAC GGACTGACAC TCTGCTGCCT GCGGTACGTG
    TGGGCCATGG AGCTCCCCGA GCTGCCCACC ACCCTGGGCC CTGTCAGTCT ACATCAGTTG
    GGATTGGAGC ATACACGCTA CCCCTGCCTG GACCTTGGTG CCATGCTGCT GTATCTGCTC
    ACGTTCTGGC TGCTCCTGCG TCAGTTCGTG AAGGAGAAGC TGCTGAAGAA GAGAAAGGCA
    CCTTCGACGC TGCTGGAGGT CACAGTGTCC GACACAGAGC CCACACAGAC CCAGACACTG
    CTGAGGAGCC TGGGGGAGCT GGTCACGGGC ATCTACGTCA AGTACTGGAT CTACGTGTGC
    GCTGGGATGT TCATTGTGGT CAGCTTCGCT GGCCGCCTGG TGGTCTACAA AATCGTCTAC
    ATGTTCCTTT TCCTGCTGTG CCTCACGTTG TTCCAGGTCT ACTACACTCT GTGGAGGAAG
    CTGCTCCGAG TTTTCTGGTG GCTCGTGGTG GCCTACACCA TGCTCGTGCT CATCGCCGTG
    TACACTTTCC AGTTCCAGGA CTTCCCCACC TACTGGCGCA ACCTCACGGG CTTCACAGAT
    GAACAGTTGG GCGACCTGGG CCTGGAGCAG TTCAGCGTGT CGGAGCTCTT TTCCAGCATC
    CTCATCCCTG GCTTCTTCCT GCTGGCCTGC ATCCTGCAGC TGCACTACTT CCACAGGCCG
    TTCATGCAGC TCACCGACCT GGAGCACGTG CCCCCGCCAG GCACACGCCG CCTTCGCTGG
    GCTCACAGGC AGGATACAGT GAGCGAGGCC CCTCTGCTTC AGCATCAGGA GGAGGAGGAA
    GTCTTCAGGG ATGACGGGCA GAGCATGGAT GGGCCCCACC AGACCACACA GGTCCCTGAG
    GGTACAGCCA GCAAGTGGGG CCTGGTGGCC GACCGGCTGC TGGACCTAGC GTCCAGCTTC
    TCCGCCGTCC TCACCCGCAT CCAGGTGTTC GTACGATGTT TGCTCGAGCT TCACGTCTTC
    AAGCTGGTGG CTCTCTACAC TGTCTGGGTG GCCCTGAAAG AAGTGTCCGT GATGAACCTG
    CTGCTGGTGG TGCTGTGGGC CTTCGCCCTG CCCTACCCGC GCTTCCGGCC CATGGCCTCC
    TGCCTGTCCA CCGTGTGGAC CTGTATCATC ATCGTGTGCA AGATGCTGTA TCAGCTGAAG
    ATTGTCAACC CACACGAGTA CTCCAGTAAC TGCACCGAGC CCTTTCCCAA CAATACCAAC
    TTGCAGCCCT TGGAGATCAG CCAGTCTCTG CTGTACCGTG GCCCCGTTGA CCCTGCCAAC
    TGGTTTGGGG TGCGGAAGGG TTACCCAAAC TTGGGCTATA TCCAGAACCA CCTGCAGATC
    CTTCTGCTGC TGGTGTTTGA GGCTGTGGTG TACCGGCGCC AAGAGCACTA CCGCCGGCAG
    CACCAGCAGG CCCCTCTGCC CGCCCAGGCT TTGTGCGCTG ATGGCACCCG CCAGAGGCTG
    GACCAGGACC TACTCAGCTG CCTCAAATAT TTCATCAACT TCTTCTTCTA CAAATTCGGG
    CTGGAGATCT GCTTCTTGAT GGCCGTGAAT GTCATCGGAC AACGCATGAA CTTCATGGTG
    ATCTTACACG GCTGCTGGTT GGTGGCCATC CTTACACGCC GGCGCCGTGA GGCCATCGCC
    CGCCTCTGGC CCAATTACTG TCTGTTCCTC ACGCTGTTCC TGCTGTACCA GTACCTGCTG
    TGCCTGGGCA TGCCTCCCGC ACTGTGCATC GACTACCCGT GGCGCTGGAG CCAGGCCATC
    CCCATGAATT CTGCGCTCAT CAAGTGGCTG TACCTCCCTG ACTTCTTCAG GGCCCCCAAC
    TCCACCAACC TTATCAGTGA CTTCCTCCTG CTACTCTGCG CCTCCCAACA GTGGCAGGTG
    TTCTCCGCAG AGCAAACGGA AGAATGGCAG CGCATGGCTG GCGTTAACAC TGACCACCTG
    GAGCCCCTGC GTGGGGAGCC CAACCCTATC CCCAACTTCA TCCACTGCAG GTCCTATCTG
    GATATGCTGA AGGTGGCCGT CTTCCGCTAC CTGTTCTGGC TGGTGCTGGT GGTCGTGTTT
    GTCACAGGGG CCACCCGCAT CAGCATCTTT GGGCTGGGGT ACCTGCTGGC CTGCTTCTAC
    CTGCTGCTCT TTGGCACCAC CCTGCTGCAG AAGGACACAC GAGCCCAGCT CGTGCTGTGG
    GACTGCCTCA TCCTCTACAA CGTCACCGTC ATCATCTCTA AGAATATGTT GTCGCTCCTG
    TCCTGTGTCT TCGTGGAGCA AATGCAGAGC AACTTCTGCT GGGTCATCCA GCTCTTCAGC
    CTCGTGTGCA CAGTCAAAGG CTACTATGAC CCCAAAGAGA TGAAGACCAG GGACCGGGAT
    TGCCTGCTGC CTGTGGAGGA GGCCGGGATC ATCTGGGACA GCATCTGCTT CTTCTTCCTG
    CTCCTACAAC GGCGCGTCTT TCTCAGCCAC TACTTCCTGC ACGTCAGTGC TGACCTGAAG
    GCCACGGCCC TGCAGGCCTC CAGGGGTTTT GCTCTCTACA ATGCGGCCAA CATAAAGAAC
    ATCAACTTCC ACCGTCAGAC CGAGGAGAGG TCCCTGGCCC AGCTGAAAAG ACAGATGAAG
    CGCATCCGTG CCAAACAGGA GAAATACCGG CAGAGCCAGG CAAGCCGTGG TCAACTCCAG
    TCCACAGACC CTCAGGAGCC AGGGCCTGAC AGCCCAGGGG GTTCCTCCCC GCCACGGACA
    CAGTGGTGGC GCCCCTGGCA GGACCACGCC ACAGTCATCC ACTCTGGTGA CTACTTCCTG
    TTTGAGTCGG ACAGTGAGGA GGAAGAGGAG GCCCTGCCTG AGGACCCCAG GCCTGCAGCT
    CAGAGTGCCT TCCAGATGGC ATACCAGGCA TGGGTAACCA ACGCCCAGAC AGTGCTAAGG
    CAGCGTCGTG AACAGGCACG GCGGGATCGG GCAGAACAGC TGGCTTCTGG AGGCGACTTG
    AGTCCTGAGG TGGAGCCGGT AGACGTCCCA GAGAATGAGA TGGCAGGCCG TAGCCATGTG
    ATGCAGCGCG TGCTGAGTAC CATGCAGTTC CTGTGGGTGC TGGGCCAGGC CACAGTGGAC
    GGGCTGACGC GCTGGTTGCG TACGTTCACG AAGGACCACC GCACCATGAG TGATGTGCTG
    TGTGCAGAGC GTTACCTGCT CACCCAGGAG CTTCTTCGGG GTGGAGAGGT ACACCGAGGG
    GTACTGGACC AGCTTTACGT GAGCGAAGAT GAGATCGCAC TGTCAGGTCC TGTGGAGAAC
    CGTGATGGAC CCAGCACAGC CTCTAGCGGG CTGGGAGCTG AAGAGCCTTT GAGTAGCATG
    ACAGACGACA CCGGCAGCCC CCTTAGCACT GGCTACAACA CTCGCAGTGG TAGTGAGGAG
    ATTATCACCG ACACTGGGGG CCTCCAGGCT GGGACCTCCC TGCACGGCTC CCAAGAGCTT
    CTAGCCAATG CTCGTACCCG GATGCGCACA GCCAGTGAGC TGCTGCTGGA CAGGCGCCTG
    CGTATCCCTG AGCTGGAGGA GGCCGAGCAG TTTGAGGCAC AGCAGGGCCG GACGCTGCGG
    CTGCTCAGAG CCATGTACCA GTGTGTGGCG GCCCACTCAG AGCTTCTCTG TTACTTCATC
    ATCATCCTTA ACCACATGGT GACAGCCTCG GCTGCCTCCC TGGTGCTGCC TGTGCTTGTG
    TTCCTCTGGG CCATGCTGAC CATCCCGAGG CCTAGCAAGC GCTTCTGGAT GACAGCTATC
    GTCTTCACTG AGGTCATGGT GGTCACCAAA TACTTGTTCC AGTTTGGCTT CTTCCCCTGG
    AACAGCTACA TAGTGCTTCG ACGCTACGAG AACAAGCCCT ACTTCCCTCC GCGCATCCTG
    GGCCTCGAGA AAACGGACAG CTATATCAAG TACGACCTGG TGCAACTCAT GGCCCTCTTC
    TTCCACCGCT CGCAGTTACT GTGCTATGGC CTCTGGGACC ATGAGGAGGA TGGTGTCCCC
    AAGGACCATT GCAGGAGTAG CGAAAAGGAC CAAGAAGCTG AGGAAGAGTC AGAGGCTAAG
    CTGGAATCGC AGCCTGAGAC GGGCACTGGG CATCCTGAGG AGCCCGTGTT GACTGGTACC
    CCCAAGGACC ACATCCAAGG GAAAGGAAGT GTTAGGTCCA AGGATGAGAT CCAAGATCCC
    CCAGAGGACC TTAAGCCCCA GCACAGGAGA CACATCAGCA TACGCTTCAG GAGGAGGAAG
    GAGACTCAAG GACCCAAAGG AGCAGCAGTC GTGGAGGCTG AGCACGAGGA GGGAGAGGAA
    GGAAGAGAAG CTGCAGGGAG AAAGAGGCTG CGTCGCCCTC GAGAAGGGTT GAAGATTCGG
    GAGAAAATGA AGGCAGCTGG ACGCCGGCTG CAGAGCTTCT GTCTGTCACT GGCCCAGAGC
    TTTTACCAAC CCCTGCGGCG TTTCTTCCAT GACATTCTGC ACACCAAGTA CCGAGCGGCC
    ACCGACGTCT ATGCCCTCAT GTTCCTGGCT GACATTGTCG ACATTGTCGT CATCATCTTC
    GGTTTTTGGG CTTTTGGGAA GCACTCCGCG GCCACGGACA TTGCATCCTC ACTGTCAGAT
    GACCAGGTGC CACAGGCCTT CCTGTTCATG CTGCTGGTCC AGTTCGGCAC CATGGTCATA
    GACCGCGCCC TCTACCTGCG TAAGACTGTA CTGGGCAAGC TGGCCTTTCA GGTGGTCCTG
    GTGGTGGCCA TTCACCTCTG GATGTTTTTC ATCTTACCGG CTGTCACCGA GAGGATGTTC
    AGACAGAACG CGGTGGCCCA GCTGTGGTAC TTCGTGAAGT GCATTTACTT TGCCCTGTCC
    GCCTACCAGA TCCGCTGTGG CTACCCCACC CGTATCTTGG GCAACTTCCT CACCAAGAAG
    TACAATCATC TAAACCTCTT CCTTTTCCAG GGGTTCCGTC TAGTGCCATT CCTGGTGGAG
    CTGCGGGCCG TCATGGACTG GGTATGGACT GACACCACGC TGTCCCTGTC CAACTGGATG
    TGTGTGGAAG ACATCTATGC CAACATCTTT ATCATCAAGT GCAGCCGAGA GACAGAGAAG
    AAATACCCCC AGCCCAAGGG ACAGAAGAAG AAGAAAATCG TCAAGTATGG CATGGGAGGT
    CTCATCATCC TCTTCCTCAT TGCCATCATC TGGTTCCCTC TGCTCTTCAT GTCACTGATC
    CGCTCTGTGG TTGGTGTCGT CAACCAGCCC ATTGATGTCA CTGTTACCCT CAAGTTAGGT
    GGCTACGAGC CACTGTTCAC CATGAGCGCC CAGCAGCCAT CCATAGTGCC ATTCACTCCC
    GAGGACTATG AGGAGCTGTC CCAGCAGTTT GACCCCTACC CACTAGCCAT GCAGTTCATT
    AGCCAGTACA GTCCTGAGGA CATCGTCACT GCACAGATTG AGGGCAGCTC GGGGGCCCTG
    TGGCGCATCA GCCCGCCCAG CCGGGCCCAG ATGAAGCACG AGCTGTACAA CGGCACAGCC
    GACATCACAC TGCGCTTCAC CTGGAATTTC CAAAGGGACC TGGCCAAGGG CGGCTCTGTG
    GAGTATACCA ACGAGAAGCA CACCTTGGAG CTGGCCCCCA ACAGTACGGC ACGAAGGCAG
    CTGGCCCAAC TGCTCGAGGG CAGACCTGAC CAGTCAGTGG TCATTCCCCA CCTCTTCCCC
    AAGTACATTC GTGCACCCAA TGGGCCTGAA GCCAATCCTG TGAAGCAACT GCAGCCAGAC
    GAGGAAGAGG ACTACCTTGG TGTGCGTATC CAGCTGCGGA GAGAGCAAGT GGGCACAGGA
    ACCTCTGGGG AGCAAGCAGG CACAAAGGCC TCCGACTTCC TCGAGTGGTG GGTGATTGAG
    CTGCAGGACT GCCAGGCCGA GTGCAACCTG CTGCCCATGG TCATCTTCAG TGACAAGGTC
    AGCCCGCCTA GCCTGGGCTT CCTGGCCGGC TACGGGATTG TGGGGCTCTA CGTCTCTATC
    GTGCTGGTCG TTGGCAAGTT TGTGCGGGGC TTCTTCAGTG ATATCTCACA CTCCATCATG
    TTCGAGGAGC TGCCGTGCGT GGACCGCATC CTCAAGCTGT GCCAGGACAT CTTCTTGGTA
    CGGGAGACCC GAGAGCTGGA GCTGGAGGAG GAACTCTATG CCAAGCTCAT ATTCCTGTAC
    CGATCACCAG AGACCATGAT CAAGTGGACG CGTGAGAAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.