Synm cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Synm
Entrez Gene ID	233335
Full Name	synemin, intermediate filament protein
Synonyms	4930412K21Rik,AI852401,Dmn,E130104F11,Syn,Synemin
General protein information	Preferred Names synemin, intermediate filament protein Names synemin synm
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_201639.2	NP_964001.2	synemin isoform H
NM_207663.3	NP_997546.2	synemin isoform M
NM_183312.3	NP_899135.3	synemin isoform L

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SYNM	XP_003314921.1
	SYNM	XP_001108469.2
Homo sapiens (human)	SYNM	NP_663780.2
Bos taurus (cattle)	SYNM	NP_001178382.1
Danio rerio (zebrafish)	synm	XP_005159213.1
Rattus norvegicus (Norway rat)	Synm	NP_001128330.1
Xenopus tropicalis (tropical clawed frog)	synm	XP_002939315.2
Mus musculus (house mouse)	Synm	NP_964001.2
Gallus gallus (chicken)	SYNM	NP_990140.1

Gene Ontology
Bibliography

Related articles in PubMed

Absence of synemin in mice causes structural and functional abnormalities in heart.
Garc?a-Pelagio KP, Chen L, Joca HC, Ward C, Jonathan Lederer W, Bloch RJ
Journal of molecular and cellular cardiology114354-363(2017 Dec)

Vimentin knockout results in increased expression of sub-endothelial basement membrane components and carotid stiffness in mice.
Langlois B, Belozertseva E, Parlakian A, Bourhim M, Gao-Li J, Blanc J, Tian L, Coletti D, Labat C, Ramdame-Cherif Z, Challande P, Regnault V, Lacolley P, Zhenlin L
Scientific reports7(1)11628(2017 Sep)

Deficiency of the intermediate filament synemin reduces bone mass in vivo.
Moorer MC, Buo AM, Garcia-Pelagio KP, Stains JP, Bloch RJ
American journal of physiology. Cell physiology311(6)C839-C845(2016 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Myopathic changes in murine skeletal muscle lacking synemin.
Garc?a-Pelagio KP, Muriel J, O'Neill A, Desmond PF, Lovering RM, Lund L, Bond M, Bloch RJ
American journal of physiology. Cell physiology308(6)C448-62(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suggest that synemin contributes to linkage between costameres and the contractile apparatus and that the absence of synemin results in decreased fiber size and increased sarcolemmal deformability and susceptibility to injury.
Title: Myopathic changes in murine skeletal muscle lacking synemin.

alpha-Synemin localizes to the M-band of the sarcomere through interaction with the M10 region of titin
Title: ?-Synemin localizes to the M-band of the sarcomere through interaction with the M10 region of titin.

results show that synemin is necessary to maintain membrane integrity and regulates signalling molecules during muscle hypertrophy.
Title: Synemin acts as a regulator of signalling molecules during skeletal muscle hypertrophy.

These analyses show that synemin transcript and its encoded protein colocalize in the anterior horn cells and that no differences in synemin expression were found in nerve tissue from C57BL/6 or mdx mice.
Title: Expression of synemin in the mouse spinal cord.

Synemin isoforms during mouse development.
Title: Synemin isoforms during mouse development: multiplicity of partners in vascular and neuronal systems.

The following Synm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Synm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09624	NM_201639.2 Latest version!	Mus musculus synemin, intermediate filament protein (Synm), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$916.30 $1309.00
OMu10105	NM_207663.3 Latest version!	Mus musculus synemin, intermediate filament protein (Synm), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu58365	NM_183312.3 Latest version!	Mus musculus synemin, intermediate filament protein (Synm), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Synm ( NM_201639.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_964001.2 Mus musculus synemin, intermediate filament protein (Synm), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09624
Clone ID Related Accession (Same CDS sequence)	NM_201639.2
Accession Version	NM_201639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Mus musculus(house mouse)
Product	synemin isoform H
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143019.1 and AC158296.6. On Jul 25, 2007 this sequence version replaced NM_201639.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (H). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ579700.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133 [ECO:0000348] ##Evidence-Data-END##

ATGCTGTCCT GGCGGCTGCA GACGGGCTCT GAAAAGGCGG AGCTGCAGGA GCTCAACGCC 
CGGCTTTACG ACTACGTGTG CCGGGTGCGA GAGCTGGAGC GCGAGAACCT GCTACTGGAG 
GAGGAGTTGC GCAGCCGGCT GAGCCGGGAG GACCGCTGGG CTGAGGATCA GGCACTCTAC 
GCCGAGGAGG CGCGCAGCCT ACGACAGCAG CTAGATGAAC TGAACTGGTC CACAGCCCTG 
GCCGAGGGCG AGCGCGACGC GCTGCGGAGG GAACTGCTGG AGCTGCAGCG GGAGGGTGTG 
GAAGCCGGCA CTGCCCGCAG CCGCCTGGAT GCCGAGCTGG GAGCGCAACG GCGGGAGCTA 
GAGGAGGCTC TGGGCGCGCG CGCCGCCCTC GAGGCGCTGC TGGGCCGGCT GGAAACTGAG 
CGCCGCGACT TGGACGCTGC CCACGAACGC CAAGTGCGAG ATCTACGAGC CCGCGCTGCC 
AGCCTCACCA TGCACTTCCG AGCCCGCGCC ACCAGCCCCG CCGCGCCCCC GCCGCGCCTG 
CGGGACGTGC ACGACAGCTA CGCCCTGCTG GTGGCCGAGT CGTGGAGGGA GAGCGTGCAG 
CTGTACGAGG ACGAGGTGCG CGAGCTGGAG CAGGCGCTGC GCCGCGGCCA AGAGAGCCGG 
CTCCAGGCGG AGGACGAGGC GCGGCTGTGC GCGCAGGAGG CAGACGCGCT GCGGAACCAG 
GCGCTGGAGT TGGAGCAGCT GCGCGCGAGG CTGGAGGATG AGCTGCTGCG GATGCGCGAG 
GAGTACGGGA TGCAGGCGGA GGAGCGGCAG AGAGTGATTG ACAGCCTGGA GGATGAGAAG 
GAAGCCCTTA CCTTGGCCAT GGCTGACCGG CTGCGGGATT ACCAGGAGCT CCTGCAGGTG 
AAGACTGGAC TCAGCTTGGA GGTAGCCACC TACAGGGCCT TGTTGGAAGG AGAAAGCAAT 
CCGGAGATAT TGATCTGGAC TGAGAACATT GAAAACGTGC CACAAGAGCC CAGAAACACA 
TCCTATCGCT ACACCAACTC AGTATTGCAG AGGAAAAATG AGAAAAATCT CTTTCCAAGG 
CGAAAAACAC CTTGGGCAGC TGTAAATCAC AGCTCGGCCT CGTATTCTAA CTGGCCAGGG 
CACCTTGACT CACAGACAAC CACAGCTGTA GGAAGTGCTG CCAGAAGGGG TCTCCTGACC 
TCCCGCCATT CTTCCTCAGC TACAACCTCA GGGCAGCAAA AGCCCCTGGA GAAAACCATC 
AGCAGCCGAG CCAATTTAAG ACCGGTCACG CCAACCCACG GCTTTCTAAG AAACACTGAT 
GCTCAAATGA AAACACTCCC CCATAGATCC AAAGTCGAAG GCACGGGGGA CACCCACGCA 
CGGCGGGCTA CAGAGTCTGT TATTACCAGA GAGTCATACA GAGGTCACCA GGGCCATGTG 
GCAGCAGGTG CCGTGAGCAG CACTCCGTCA AATGAGAGGA CTGTCATTCT GGGAAAGAAA 
TTAGAAGCGC AAGCCACTAA AGAACAAGAA AGGGACAGAT CAGGGGTCAT CAGAATAAAG 
CCAGAAGAGA AAATGTTTGA TTCTAAAGAG AAGGCTTCAG AGGAAAGAAA CTTGAGGTGG 
GAAGAGCTAA CCAAGTTAGA TAGAGATGCC AGAAAGAGAG AAAGCCGGCA TTTGAGGGAT 
GAGGCCAGAG AGAAGGAAGC ACTGAAGGAG AGAAGTGTAA AAGAAAGAGA GGTGCCCATC 
AGTCTAGAAG TATCCCGGGG CAGCAGAGCA GAGGTGTCCA CCATACACTT GCAGTCACCT 
GGAAGGAAGG ACGTTAGCCA CAGTGGGGGA AGAGAGGCAG AGACCAAAGA GACAAGGTTC 
CGGCTGGATA CCCAGGACAC TGCCAGCTCT CTGCAAAGTG ACTCAACGAC AGAAACCATA 
GCCGAAAGCA TTGTCACCAC CATCCTTAAG CAGTTTACCC AGTCTCCAGG TGCCGAGGAG 
GAGGCCACTT CTTTCCCGGA CACAAAGGTC ACTTATGTGG ACAGGAAAGA GTTTCCTGGT 
GATGGGAAAA CCAAGACAGA AATCGTTGTG GAGTCGAAGC TGACAGATGT TGTTGATGTT 
TCTGATGAAG CAGGCCTGGA CTACCTTCTA AGCAAGGATG TCAAGGAAGT AGGACTGAAG 
GGGAAGTCAA CGGAGACGAT GATCGGAGAG ATGATCAACC TGGGTCTGAA AGGCAGGGAG 
GGGAGAGCTA AAGTCGTAAA TGTGGAGATT GTGGAAGAGC CCATGAGCTA CATAGGTGGT 
GGGAAAATAG ACTTTTCTAC CCCCTTTCAG GTAGAAGAAG TCGACGACGT GTCTCCCAGC 
CCCAAGGGCT TTGTGGAGGA AGAGGATGGT GAAGGAGAGA CCCACATGGC ATTCTCAATG 
CGTCCACATC AGACCAAACA GCCCCAGGGG ACCATCCCTC ACGTGGAAGA AGTGACGGAG 
GCGGGCGACT CAGAGGGGGA GCAGAGCTAT TTTGTGTCTA CCCCAGATGA GTACCCCGGG 
GGGCATGACA GAGAAGATGA TGGCTCAGTG TATGGGCAGA TTCATATCGA GGAAGAGTCT 
ACCATCCGGT ACTCATGGCA AGACGAAATT GCGCAGGGGA CCTGGAGAAG GAAAATGAGA 
GGTGACGTGG GGGGAGAGAA GCCTGTGAAG GTCCTGGAGG TCCCAGCACT TTCCCTGGGT 
GGTGCTATTG GTTCTGCTCA CTTGAAGGAA GAAGCCAGTG GTGAACTCCG TGCTGAACCC 
ACAGTCATTG AGAAAGAGAT CAAAATACCG CATGAATTCC ACACCTCCAT CAAGGGTGTC 
TTCTCCAGTG AGCCCCGGCA CCAGCTGGTG GAGGTCATCG GGCAGCTGGA GGAAACATTG 
CCCGAGCGCA TGAAGGAGGA GCTGTCTGCC CTGACCAGAC AGAGTCAAGG AGAGTCAGGG 
AGCGTTTCTG TGGACGTAAA GAAAGTCCAG AGCGCTGCTG GTGGTTCTGT GACTTTGATG 
GCTGAGGTCA ACCTCTCACA GACTGTAGAT GCTGATCAGT TGGACCTGGA GCAGTTGAGC 
AGAGATGAAG CTGGGGAAAT AGAGAGGGCT GTGGAGTCTG TGGTCAGAGA GAGCTTGGCC 
AAGCGCTCCA GCCCAGTGCC TAGAAGCCCA GACAGGGAAG ATGGAGAGGA GGTGCCTGCT 
GGTGGCATTC TCTTCAAGCG CTGGGCCACC AGGGAGCTGT ATAGCCCATC TGGTGAGAGG 
GATGATGCTG GCCAGGTCTC TCCCAGCTCA GATCAGCGTG TTACCCAGGG CCCAGTGTCA 
GCTACTGTGG AAGTGACCAG CCCAACGGGT TTTGTACAGT CTCATGTGCT GGAGGATGTG 
AGCCAGTCTG TAAGGCATGT TAAACTAGGT CCCACTGAAA TGTGGAGGAC AGAACAGGTG 
ACCTTCGGAG GACCCACCGC ACAGGTGGTG GAGGTAAGTG GAGACTTCAG CGAGGCAGTC 
AGCTCTGAGG GAGCCAGCCG CTCTGTGAGG CACATTACAC TGGGTCCCCA TCAAAGCCAA 
GTGTCCACAG AAGTCATTTT CCGAGGCTCT GTGCCTACCT GGCAGGAAAC AGGAGACACA 
GAAAAGCCTG GCCCAGTGGT ACTGTCTGTG GGAGCTGACA TCTCAGGGAG CGGCAGGATG 
CCTGGCTCTG AGCGGTCTCA CACTGAGAAA GAAATTCGTT TTCAGGGTCC TGTTTCTGGG 
ACAGCCCAGG TTGGTGGTAA CTTTGCAACT GAAGAGTCAG TGGGTAGCCA GACTTTTGTA 
AGGAGTCTCC AGTTAGGCCC CAAAGAAGGG TTCAGAGAGG AAATCCAGTT CATAGCTCCT 
ATCCCAGACA AGGTAGGGTG GGGAGAAGAG GATTCTGAGC ACACCAAAGT GTCGCTAGAG 
AGGGCTACAT CCATCCAGCG TATTGACATT GTGCCCCAGA GGTACCTAGC TTCCAAGCAG 
ATGGCCCCAC AGACATTGGA ATTCAGGGAC TCAGAAGACA TGGTTATGGT GGAAGGTTCA 
GCAGGCACAA TCCAGGCCAC TCACAATTTC ACCTCAGATA GAGAAATCCT CCAGAACAAA 
GAGAACACAT TCCAGAGGGT CATTTCTGGG TCTCCTCCAG ACAGTGTGGG CGACACGGGA 
GCAGAGGTGA CAGCAAACGT CAGCAGGTCC TTTCGGCACA TTCAAATAGG TCCTACAGAG 
GAAGAACCCT CTGAATACTT TGTCACCGGG AGACCCGTAT CTAAAACATT TGTACTTGAT 
GGTTCCGTGG CGTCCCCTGG GCTGGTTGGG GGAGCAGACG GTGGTAGCAC ACCGTGTCGG 
ATTGCACTGG GGCCCAAAGA AACTTCATTT ACTTTCCAGA TGGATCTGAG CGACACGAGA 
GCGATCCGCA GCTGGACCCG AGACACAGGG TCGGAAGTGG AAGCTCACGG CGTGTCTCAC 
CGTGGTGGCT GGAGAATTGC ACACAGTAGG GATGAGCGAG TGGCCAGCAC TGGCTCTGGG 
GCCTCCCCTG GGGATGCACA CCAGGCCCCT GGAGAGAAAG GCACGGAGCA GGCTGGATTT 
GACAAGACGG TACAGTTGCA GAGAATGGTA GACCAAAGGT CGGTGGCCTC AGATGAGAAG 
AAAGTGGCTC TTCTTTATCT AGACAATGAA GAGGAGGAGG AGGAGGAGGG CGAAGGGTGG 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_964001.2
CDS	115..4800
Translation	MLSWRLQTGSEKAELQELNARLYDYVCRVRELERENLLLEEELRSRLSREDRWAEDQALYAEEARSLRQQLDELNWSTALAEGERDALRRELLELQREGVEAGTARSRLDAELGAQRRELEEALGARAALEALLGRLETERRDLDAAHERQVRDLRARAASLTMHFRARATSPAAPPPRLRDVHDSYALLVAESWRESVQLYEDEVRELEQALRRGQESRLQAEDEARLCAQEADALRNQALELEQLRARLEDELLRMREEYGMQAEERQRVIDSLEDEKEALTLAMADRLRDYQELLQVKTGLSLEVATYRALLEGESNPEILIWTENIENVPQEPRNTSYRYTNSVLQRKNEKNLFPRRKTPWAAVNHSSASYSNWPGHLDSQTTTAVGSAARRGLLTSRHSSSATTSGQQKPLEKTISSRANLRPVTPTHGFLRNTDAQMKTLPHRSKVEGTGDTHARRATESVITRESYRGHQGHVAAGAVSSTPSNERTVILGKKLEAQATKEQERDRSGVIRIKPEEKMFDSKEKASEERNLRWEELTKLDRDARKRESRHLRDEAREKEALKERSVKEREVPISLEVSRGSRAEVSTIHLQSPGRKDVSHSGGREAETKETRFRLDTQDTASSLQSDSTTETIAESIVTTILKQFTQSPGAEEEATSFPDTKVTYVDRKEFPGDGKTKTEIVVESKLTDVVDVSDEAGLDYLLSKDVKEVGLKGKSTETMIGEMINLGLKGREGRAKVVNVEIVEEPMSYIGGGKIDFSTPFQVEEVDDVSPSPKGFVEEEDGEGETHMAFSMRPHQTKQPQGTIPHVEEVTEAGDSEGEQSYFVSTPDEYPGGHDREDDGSVYGQIHIEEESTIRYSWQDEIAQGTWRRKMRGDVGGEKPVKVLEVPALSLGGAIGSAHLKEEASGELRAEPTVIEKEIKIPHEFHTSIKGVFSSEPRHQLVEVIGQLEETLPERMKEELSALTRQSQGESGSVSVDVKKVQSAAGGSVTLMAEVNLSQTVDADQLDLEQLSRDEAGEIERAVESVVRESLAKRSSPVPRSPDREDGEEVPAGGILFKRWATRELYSPSGERDDAGQVSPSSDQRVTQGPVSATVEVTSPTGFVQSHVLEDVSQSVRHVKLGPTEMWRTEQVTFGGPTAQVVEVSGDFSEAVSSEGASRSVRHITLGPHQSQVSTEVIFRGSVPTWQETGDTEKPGPVVLSVGADISGSGRMPGSERSHTEKEIRFQGPVSGTAQVGGNFATEESVGSQTFVRSLQLGPKEGFREEIQFIAPIPDKVGWGEEDSEHTKVSLERATSIQRIDIVPQRYLASKQMAPQTLEFRDSEDMVMVEGSAGTIQATHNFTSDREILQNKENTFQRVISGSPPDSVGDTGAEVTANVSRSFRHIQIGPTEEEPSEYFVTGRPVSKTFVLDGSVASPGLVGGADGGSTPCRIALGPKETSFTFQMDLSDTRAIRSWTRDTGSEVEAHGVSHRGGWRIAHSRDERVASTGSGASPGDAHQAPGEKGTEQAGFDKTVQLQRMVDQRSVASDEKKVALLYLDNEEEEEEEGEGWF

Target ORF information:

RefSeq Version	NM_201639.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synemin, intermediate filament protein (Synm), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201639.2

ORF Insert Sequence:

ATGCTGTCCT GGCGGCTGCA GACGGGCTCT GAAAAGGCGG AGCTGCAGGA GCTCAACGCC 
CGGCTTTACG ACTACGTGTG CCGGGTGCGA GAGCTGGAGC GCGAGAACCT GCTACTGGAG 
GAGGAGTTGC GCAGCCGGCT GAGCCGGGAG GACCGCTGGG CTGAGGATCA GGCACTCTAC 
GCCGAGGAGG CGCGCAGCCT ACGACAGCAG CTAGATGAAC TGAACTGGTC CACAGCCCTG 
GCCGAGGGCG AGCGCGACGC GCTGCGGAGG GAACTGCTGG AGCTGCAGCG GGAGGGTGTG 
GAAGCCGGCA CTGCCCGCAG CCGCCTGGAT GCCGAGCTGG GAGCGCAACG GCGGGAGCTA 
GAGGAGGCTC TGGGCGCGCG CGCCGCCCTC GAGGCGCTGC TGGGCCGGCT GGAAACTGAG 
CGCCGCGACT TGGACGCTGC CCACGAACGC CAAGTGCGAG ATCTACGAGC CCGCGCTGCC 
AGCCTCACCA TGCACTTCCG AGCCCGCGCC ACCAGCCCCG CCGCGCCCCC GCCGCGCCTG 
CGGGACGTGC ACGACAGCTA CGCCCTGCTG GTGGCCGAGT CGTGGAGGGA GAGCGTGCAG 
CTGTACGAGG ACGAGGTGCG CGAGCTGGAG CAGGCGCTGC GCCGCGGCCA AGAGAGCCGG 
CTCCAGGCGG AGGACGAGGC GCGGCTGTGC GCGCAGGAGG CAGACGCGCT GCGGAACCAG 
GCGCTGGAGT TGGAGCAGCT GCGCGCGAGG CTGGAGGATG AGCTGCTGCG GATGCGCGAG 
GAGTACGGGA TGCAGGCGGA GGAGCGGCAG AGAGTGATTG ACAGCCTGGA GGATGAGAAG 
GAAGCCCTTA CCTTGGCCAT GGCTGACCGG CTGCGGGATT ACCAGGAGCT CCTGCAGGTG 
AAGACTGGAC TCAGCTTGGA GGTAGCCACC TACAGGGCCT TGTTGGAAGG AGAAAGCAAT 
CCGGAGATAT TGATCTGGAC TGAGAACATT GAAAACGTGC CACAAGAGCC CAGAAACACA 
TCCTATCGCT ACACCAACTC AGTATTGCAG AGGAAAAATG AGAAAAATCT CTTTCCAAGG 
CGAAAAACAC CTTGGGCAGC TGTAAATCAC AGCTCGGCCT CGTATTCTAA CTGGCCAGGG 
CACCTTGACT CACAGACAAC CACAGCTGTA GGAAGTGCTG CCAGAAGGGG TCTCCTGACC 
TCCCGCCATT CTTCCTCAGC TACAACCTCA GGGCAGCAAA AGCCCCTGGA GAAAACCATC 
AGCAGCCGAG CCAATTTAAG ACCGGTCACG CCAACCCACG GCTTTCTAAG AAACACTGAT 
GCTCAAATGA AAACACTCCC CCATAGATCC AAAGTCGAAG GCACGGGGGA CACCCACGCA 
CGGCGGGCTA CAGAGTCTGT TATTACCAGA GAGTCATACA GAGGTCACCA GGGCCATGTG 
GCAGCAGGTG CCGTGAGCAG CACTCCGTCA AATGAGAGGA CTGTCATTCT GGGAAAGAAA 
TTAGAAGCGC AAGCCACTAA AGAACAAGAA AGGGACAGAT CAGGGGTCAT CAGAATAAAG 
CCAGAAGAGA AAATGTTTGA TTCTAAAGAG AAGGCTTCAG AGGAAAGAAA CTTGAGGTGG 
GAAGAGCTAA CCAAGTTAGA TAGAGATGCC AGAAAGAGAG AAAGCCGGCA TTTGAGGGAT 
GAGGCCAGAG AGAAGGAAGC ACTGAAGGAG AGAAGTGTAA AAGAAAGAGA GGTGCCCATC 
AGTCTAGAAG TATCCCGGGG CAGCAGAGCA GAGGTGTCCA CCATACACTT GCAGTCACCT 
GGAAGGAAGG ACGTTAGCCA CAGTGGGGGA AGAGAGGCAG AGACCAAAGA GACAAGGTTC 
CGGCTGGATA CCCAGGACAC TGCCAGCTCT CTGCAAAGTG ACTCAACGAC AGAAACCATA 
GCCGAAAGCA TTGTCACCAC CATCCTTAAG CAGTTTACCC AGTCTCCAGG TGCCGAGGAG 
GAGGCCACTT CTTTCCCGGA CACAAAGGTC ACTTATGTGG ACAGGAAAGA GTTTCCTGGT 
GATGGGAAAA CCAAGACAGA AATCGTTGTG GAGTCGAAGC TGACAGATGT TGTTGATGTT 
TCTGATGAAG CAGGCCTGGA CTACCTTCTA AGCAAGGATG TCAAGGAAGT AGGACTGAAG 
GGGAAGTCAA CGGAGACGAT GATCGGAGAG ATGATCAACC TGGGTCTGAA AGGCAGGGAG 
GGGAGAGCTA AAGTCGTAAA TGTGGAGATT GTGGAAGAGC CCATGAGCTA CATAGGTGGT 
GGGAAAATAG ACTTTTCTAC CCCCTTTCAG GTAGAAGAAG TCGACGACGT GTCTCCCAGC 
CCCAAGGGCT TTGTGGAGGA AGAGGATGGT GAAGGAGAGA CCCACATGGC ATTCTCAATG 
CGTCCACATC AGACCAAACA GCCCCAGGGG ACCATCCCTC ACGTGGAAGA AGTGACGGAG 
GCGGGCGACT CAGAGGGGGA GCAGAGCTAT TTTGTGTCTA CCCCAGATGA GTACCCCGGG 
GGGCATGACA GAGAAGATGA TGGCTCAGTG TATGGGCAGA TTCATATCGA GGAAGAGTCT 
ACCATCCGGT ACTCATGGCA AGACGAAATT GCGCAGGGGA CCTGGAGAAG GAAAATGAGA 
GGTGACGTGG GGGGAGAGAA GCCTGTGAAG GTCCTGGAGG TCCCAGCACT TTCCCTGGGT 
GGTGCTATTG GTTCTGCTCA CTTGAAGGAA GAAGCCAGTG GTGAACTCCG TGCTGAACCC 
ACAGTCATTG AGAAAGAGAT CAAAATACCG CATGAATTCC ACACCTCCAT CAAGGGTGTC 
TTCTCCAGTG AGCCCCGGCA CCAGCTGGTG GAGGTCATCG GGCAGCTGGA GGAAACATTG 
CCCGAGCGCA TGAAGGAGGA GCTGTCTGCC CTGACCAGAC AGAGTCAAGG AGAGTCAGGG 
AGCGTTTCTG TGGACGTAAA GAAAGTCCAG AGCGCTGCTG GTGGTTCTGT GACTTTGATG 
GCTGAGGTCA ACCTCTCACA GACTGTAGAT GCTGATCAGT TGGACCTGGA GCAGTTGAGC 
AGAGATGAAG CTGGGGAAAT AGAGAGGGCT GTGGAGTCTG TGGTCAGAGA GAGCTTGGCC 
AAGCGCTCCA GCCCAGTGCC TAGAAGCCCA GACAGGGAAG ATGGAGAGGA GGTGCCTGCT 
GGTGGCATTC TCTTCAAGCG CTGGGCCACC AGGGAGCTGT ATAGCCCATC TGGTGAGAGG 
GATGATGCTG GCCAGGTCTC TCCCAGCTCA GATCAGCGTG TTACCCAGGG CCCAGTGTCA 
GCTACTGTGG AAGTGACCAG CCCAACGGGT TTTGTACAGT CTCATGTGCT GGAGGATGTG 
AGCCAGTCTG TAAGGCATGT TAAACTAGGT CCCACTGAAA TGTGGAGGAC AGAACAGGTG 
ACCTTCGGAG GACCCACCGC ACAGGTGGTG GAGGTAAGTG GAGACTTCAG CGAGGCAGTC 
AGCTCTGAGG GAGCCAGCCG CTCTGTGAGG CACATTACAC TGGGTCCCCA TCAAAGCCAA 
GTGTCCACAG AAGTCATTTT CCGAGGCTCT GTGCCTACCT GGCAGGAAAC AGGAGACACA 
GAAAAGCCTG GCCCAGTGGT ACTGTCTGTG GGAGCTGACA TCTCAGGGAG CGGCAGGATG 
CCTGGCTCTG AGCGGTCTCA CACTGAGAAA GAAATTCGTT TTCAGGGTCC TGTTTCTGGG 
ACAGCCCAGG TTGGTGGTAA CTTTGCAACT GAAGAGTCAG TGGGTAGCCA GACTTTTGTA 
AGGAGTCTCC AGTTAGGCCC CAAAGAAGGG TTCAGAGAGG AAATCCAGTT CATAGCTCCT 
ATCCCAGACA AGGTAGGGTG GGGAGAAGAG GATTCTGAGC ACACCAAAGT GTCGCTAGAG 
AGGGCTACAT CCATCCAGCG TATTGACATT GTGCCCCAGA GGTACCTAGC TTCCAAGCAG 
ATGGCCCCAC AGACATTGGA ATTCAGGGAC TCAGAAGACA TGGTTATGGT GGAAGGTTCA 
GCAGGCACAA TCCAGGCCAC TCACAATTTC ACCTCAGATA GAGAAATCCT CCAGAACAAA 
GAGAACACAT TCCAGAGGGT CATTTCTGGG TCTCCTCCAG ACAGTGTGGG CGACACGGGA 
GCAGAGGTGA CAGCAAACGT CAGCAGGTCC TTTCGGCACA TTCAAATAGG TCCTACAGAG 
GAAGAACCCT CTGAATACTT TGTCACCGGG AGACCCGTAT CTAAAACATT TGTACTTGAT 
GGTTCCGTGG CGTCCCCTGG GCTGGTTGGG GGAGCAGACG GTGGTAGCAC ACCGTGTCGG 
ATTGCACTGG GGCCCAAAGA AACTTCATTT ACTTTCCAGA TGGATCTGAG CGACACGAGA 
GCGATCCGCA GCTGGACCCG AGACACAGGG TCGGAAGTGG AAGCTCACGG CGTGTCTCAC 
CGTGGTGGCT GGAGAATTGC ACACAGTAGG GATGAGCGAG TGGCCAGCAC TGGCTCTGGG 
GCCTCCCCTG GGGATGCACA CCAGGCCCCT GGAGAGAAAG GCACGGAGCA GGCTGGATTT 
GACAAGACGG TACAGTTGCA GAGAATGGTA GACCAAAGGT CGGTGGCCTC AGATGAGAAG 
AAAGTGGCTC TTCTTTATCT AGACAATGAA GAGGAGGAGG AGGAGGAGGG CGAAGGGTGG 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synm ( NM_207663.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_997546.2 Mus musculus synemin, intermediate filament protein (Synm), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu10105
Clone ID Related Accession (Same CDS sequence)	NM_207663.3
Accession Version	NM_207663.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Mus musculus(house mouse)
Product	synemin isoform M
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143019.1 and AC158296.6. On Jul 25, 2007 this sequence version replaced NM_207663.2. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a shorter protein (isoform M), compared to isoform H. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ579701.1, AK143019.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCTGTCCT GGCGGCTGCA GACGGGCTCT GAAAAGGCGG AGCTGCAGGA GCTCAACGCC 
CGGCTTTACG ACTACGTGTG CCGGGTGCGA GAGCTGGAGC GCGAGAACCT GCTACTGGAG 
GAGGAGTTGC GCAGCCGGCT GAGCCGGGAG GACCGCTGGG CTGAGGATCA GGCACTCTAC 
GCCGAGGAGG CGCGCAGCCT ACGACAGCAG CTAGATGAAC TGAACTGGTC CACAGCCCTG 
GCCGAGGGCG AGCGCGACGC GCTGCGGAGG GAACTGCTGG AGCTGCAGCG GGAGGGTGTG 
GAAGCCGGCA CTGCCCGCAG CCGCCTGGAT GCCGAGCTGG GAGCGCAACG GCGGGAGCTA 
GAGGAGGCTC TGGGCGCGCG CGCCGCCCTC GAGGCGCTGC TGGGCCGGCT GGAAACTGAG 
CGCCGCGACT TGGACGCTGC CCACGAACGC CAAGTGCGAG ATCTACGAGC CCGCGCTGCC 
AGCCTCACCA TGCACTTCCG AGCCCGCGCC ACCAGCCCCG CCGCGCCCCC GCCGCGCCTG 
CGGGACGTGC ACGACAGCTA CGCCCTGCTG GTGGCCGAGT CGTGGAGGGA GAGCGTGCAG 
CTGTACGAGG ACGAGGTGCG CGAGCTGGAG CAGGCGCTGC GCCGCGGCCA AGAGAGCCGG 
CTCCAGGCGG AGGACGAGGC GCGGCTGTGC GCGCAGGAGG CAGACGCGCT GCGGAACCAG 
GCGCTGGAGT TGGAGCAGCT GCGCGCGAGG CTGGAGGATG AGCTGCTGCG GATGCGCGAG 
GAGTACGGGA TGCAGGCGGA GGAGCGGCAG AGAGTGATTG ACAGCCTGGA GGATGAGAAG 
GAAGCCCTTA CCTTGGCCAT GGCTGACCGG CTGCGGGATT ACCAGGAGCT CCTGCAGGTG 
AAGACTGGAC TCAGCTTGGA GGTAGCCACC TACAGGGCCT TGTTGGAAGG AGAAAGCAAT 
CCGGAGATAT TGATCTGGAC TGAGAACATT GAAAACGTGC CACAAGAGCC CAGAAACACA 
TCCTATCGCT ACACCAACTC AGTATTGCAG AGGAAAAATG AGAAAAATCT CTTTCCAAGG 
CGAAAAACAC CTTGGGCAGC TGTAAATCAC AGCTCGGCCT CGTATTCTAA CTGGCCAGGG 
CACCTTGACT CACAGACAAC CACAGCTGTA GGAAGTGCTG CCAGAAGGGG TCTCCTGACC 
TCCCGCCATT CTTCCTCAGC TACAACCTCA GGGCAGCAAA AGCCCCTGGA GAAAACCATC 
AGCAGCCGAG CCAATTTAAG ACCGGTCACG CCAACCCACG GCTTTCTAAG AAACACTGAT 
GCTCAAATGA AAACACTCCC CCATAGATCC AAAGTCGAAG GCACGGGGGA CACCCACGCA 
CGGCGGGCTA CAGAGTCTGT TATTACCAGA GAGTCATACA GAGGTCACCA GGGCCATGTG 
GCAGCAGGTG CCGTGAGCAG CACTCCGTCA AATGAGAGGA CTGTCATTCT GGGAAAGAAA 
TTAGAAGCGC AAGCCACTAA AGAACAAGAA AGGGACAGAT CAGGGGTCAT CAGAATAAAG 
CCAGAAGAGA AAATGTTTGA TTCTAAAGAG AAGGCTTCAG AGGAAAGAAA CTTGAGGTGG 
GAAGAGCTAA CCAAGTTAGA TAGAGATGCC AGAAAGAGAG AAAGCCGGCA TTTGAGGGAT 
GAGGCCAGAG AGAAGGAAGC ACTGAAGGAG AGAAGTGTAA AAGAAAGAGA GGTGCCCATC 
AGTCTAGAAG TATCCCGGGG CAGCAGAGCA GAGGTGTCCA CCATACACTT GCAGTCACCT 
GGAAGGAAGG ACGTTAGCCA CAGTGGGGGA AGAGAGGCAG AGACCAAAGA GACAAGGTTC 
CGGCTGGATA CCCAGGACAC TGCCAGCTCT CTGCAAAGTG ACTCAACGAC AGAAACCATA 
GCCGAAAGCA TTGTCACCAC CATCCTTAAG CAGTTTACCC AGTCTCCAGG TGCCGAGGAG 
GAGGCCACTT CTTTCCCGGA CACAAAGGTC ACTTATGTGG ACAGGAAAGA GTTTCCTGGT 
GATGGGAAAA CCAAGACAGA AATCGTTGTG GAGTCGAAGC TGACAGATGT TGTTGATGTT 
TCTGATGAAG CAGGCCTGGA CTACCTTCTA AGCAAGGATG TCAAGGAAGT AGGACTGAAG 
GGGAAGTCAA CGGAGACGAT GATCGGAGAG ATGATCAACC TGGGTCTGAA AGGCAGGGAG 
GGGAGAGCTA AAGTCGTAAA TGTGGAGATT GTGGAAGAGC CCATGAGCTA CATAGGTGGT 
GGGAAAATAG ACTTTTCTAC CCCCTTTCAG GTAGAAGAAG TCGACGACGT GTCTCCCAGC 
CCCAAGGGCT TTGTGGAGGA AGAGGATGGT GAAGGAGAGA CCCACATGGC ATTCTCAATG 
CGTCCACATC AGACCAAACA GCCCCAGGGG ACCATCCCTC ACGTGGAAGA AGTGACGGAG 
GCGGGCGACT CAGAGGGGGA GCAGAGCTAT TTTGTGTCTA CCCCAGATGA GTACCCCGGG 
GGGCATGACA GAGAAGATGA TGGCTCAGTG TATGGGCAGA TTCATATCGA GGAAGAGTCT 
ACCATCCGGT ACTCATGGCA AGACGAAATT GCGCAGGGGA CCTGGAGAAG GAAAATGAGA 
GGTGACGTGG GGGGAGAGAA GCCTGTGAAG GTCCTGGAGG TCCCAGCACT TTCCCTGGGT 
GGTGCTATTG GTTCTGCTCA CTTGAAGGAA GAAGCCAGTG GTGAACTCCG TGCTGAACCC 
ACAGTCATTG AGAAAGAGAT CAAAATACCG CATGAATTCC ACACCTCCAT CAAGGGTGTC 
TTCTCCAGTG AGCCCCGGCA CCAGCTGGTG GAGGTCATCG GGCAGCTGGA GGAAACATTG 
CCCGAGCGCA TGAAGGAGGA GCTGTCTGCC CTGACCAGAC AGAGTCAAGG AGAGTCAGGG 
AGCGTTTCTG TGGACGTAAA GAAAGTCCAG AGCGCTGCTG GTGGTTCTGT GACTTTGATG 
GCTGAGGTCA ACCTCTCACA GACTGTAGAT GCTGATCAGT TGGACCTGGA GCAGTTGAGC 
AGAGATGAAG CTGGGGAAAT AGAGAGGGCT GTGGAGTCTG TGGTCAGAGA GAGCTTGGCC 
AAGCGCTCCA GCCCAGTGCC TAGAAGCCCA GACAGGGAAG ATGGAGAGGA GGTGCCTGCT 
GGTGGCATTC TCTTCAAGCG CTGGGCCACC AGGGAGCTGT ATAGCCCATC TGGTGAGAGG 
GATGATGCTG GCCAGGTCTC TCCCAGCTCA GATCAGCGTG TTACCCAGGG CCCAGTGTCA 
GCTACTGTGG AAGTGACCAG CCCAACGGGT TTTGTACAGT CTCATGTGCT GGAGGATGTG 
AGCCAGTCTG TAAGGCATGT TAAACTAGGT CCCACTGAAA TGTGGAGGAC AGAACAGGTG 
ACCTTCGGAG GACCCACCGC ACAGGTGGTG GAGATGGATC TGAGCGACAC GAGAGCGATC 
CGCAGCTGGA CCCGAGACAC AGGGTCGGAA GTGGAAGCTC ACGGCGTGTC TCACCGTGGT 
GGCTGGAGAA TTGCACACAG TAGGGATGAG CGAGTGGCCA GCACTGGCTC TGGGGCCTCC 
CCTGGGGATG CACACCAGGC CCCTGGAGAG AAAGGCACGG AGCAGGCTGG ATTTGACAAG 
ACGGTACAGT TGCAGAGAAT GGTAGACCAA AGGTCGGTGG CCTCAGATGA GAAGAAAGTG 
GCTCTTCTTT ATCTAGACAA TGAAGAGGAG GAGGAGGAGG AGGGCGAAGG GTGGTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997546.2
CDS	115..3894
Translation	MLSWRLQTGSEKAELQELNARLYDYVCRVRELERENLLLEEELRSRLSREDRWAEDQALYAEEARSLRQQLDELNWSTALAEGERDALRRELLELQREGVEAGTARSRLDAELGAQRRELEEALGARAALEALLGRLETERRDLDAAHERQVRDLRARAASLTMHFRARATSPAAPPPRLRDVHDSYALLVAESWRESVQLYEDEVRELEQALRRGQESRLQAEDEARLCAQEADALRNQALELEQLRARLEDELLRMREEYGMQAEERQRVIDSLEDEKEALTLAMADRLRDYQELLQVKTGLSLEVATYRALLEGESNPEILIWTENIENVPQEPRNTSYRYTNSVLQRKNEKNLFPRRKTPWAAVNHSSASYSNWPGHLDSQTTTAVGSAARRGLLTSRHSSSATTSGQQKPLEKTISSRANLRPVTPTHGFLRNTDAQMKTLPHRSKVEGTGDTHARRATESVITRESYRGHQGHVAAGAVSSTPSNERTVILGKKLEAQATKEQERDRSGVIRIKPEEKMFDSKEKASEERNLRWEELTKLDRDARKRESRHLRDEAREKEALKERSVKEREVPISLEVSRGSRAEVSTIHLQSPGRKDVSHSGGREAETKETRFRLDTQDTASSLQSDSTTETIAESIVTTILKQFTQSPGAEEEATSFPDTKVTYVDRKEFPGDGKTKTEIVVESKLTDVVDVSDEAGLDYLLSKDVKEVGLKGKSTETMIGEMINLGLKGREGRAKVVNVEIVEEPMSYIGGGKIDFSTPFQVEEVDDVSPSPKGFVEEEDGEGETHMAFSMRPHQTKQPQGTIPHVEEVTEAGDSEGEQSYFVSTPDEYPGGHDREDDGSVYGQIHIEEESTIRYSWQDEIAQGTWRRKMRGDVGGEKPVKVLEVPALSLGGAIGSAHLKEEASGELRAEPTVIEKEIKIPHEFHTSIKGVFSSEPRHQLVEVIGQLEETLPERMKEELSALTRQSQGESGSVSVDVKKVQSAAGGSVTLMAEVNLSQTVDADQLDLEQLSRDEAGEIERAVESVVRESLAKRSSPVPRSPDREDGEEVPAGGILFKRWATRELYSPSGERDDAGQVSPSSDQRVTQGPVSATVEVTSPTGFVQSHVLEDVSQSVRHVKLGPTEMWRTEQVTFGGPTAQVVEMDLSDTRAIRSWTRDTGSEVEAHGVSHRGGWRIAHSRDERVASTGSGASPGDAHQAPGEKGTEQAGFDKTVQLQRMVDQRSVASDEKKVALLYLDNEEEEEEEGEGWF

Target ORF information:

RefSeq Version	NM_207663.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synemin, intermediate filament protein (Synm), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207663.3

ORF Insert Sequence:

ATGCTGTCCT GGCGGCTGCA GACGGGCTCT GAAAAGGCGG AGCTGCAGGA GCTCAACGCC 
CGGCTTTACG ACTACGTGTG CCGGGTGCGA GAGCTGGAGC GCGAGAACCT GCTACTGGAG 
GAGGAGTTGC GCAGCCGGCT GAGCCGGGAG GACCGCTGGG CTGAGGATCA GGCACTCTAC 
GCCGAGGAGG CGCGCAGCCT ACGACAGCAG CTAGATGAAC TGAACTGGTC CACAGCCCTG 
GCCGAGGGCG AGCGCGACGC GCTGCGGAGG GAACTGCTGG AGCTGCAGCG GGAGGGTGTG 
GAAGCCGGCA CTGCCCGCAG CCGCCTGGAT GCCGAGCTGG GAGCGCAACG GCGGGAGCTA 
GAGGAGGCTC TGGGCGCGCG CGCCGCCCTC GAGGCGCTGC TGGGCCGGCT GGAAACTGAG 
CGCCGCGACT TGGACGCTGC CCACGAACGC CAAGTGCGAG ATCTACGAGC CCGCGCTGCC 
AGCCTCACCA TGCACTTCCG AGCCCGCGCC ACCAGCCCCG CCGCGCCCCC GCCGCGCCTG 
CGGGACGTGC ACGACAGCTA CGCCCTGCTG GTGGCCGAGT CGTGGAGGGA GAGCGTGCAG 
CTGTACGAGG ACGAGGTGCG CGAGCTGGAG CAGGCGCTGC GCCGCGGCCA AGAGAGCCGG 
CTCCAGGCGG AGGACGAGGC GCGGCTGTGC GCGCAGGAGG CAGACGCGCT GCGGAACCAG 
GCGCTGGAGT TGGAGCAGCT GCGCGCGAGG CTGGAGGATG AGCTGCTGCG GATGCGCGAG 
GAGTACGGGA TGCAGGCGGA GGAGCGGCAG AGAGTGATTG ACAGCCTGGA GGATGAGAAG 
GAAGCCCTTA CCTTGGCCAT GGCTGACCGG CTGCGGGATT ACCAGGAGCT CCTGCAGGTG 
AAGACTGGAC TCAGCTTGGA GGTAGCCACC TACAGGGCCT TGTTGGAAGG AGAAAGCAAT 
CCGGAGATAT TGATCTGGAC TGAGAACATT GAAAACGTGC CACAAGAGCC CAGAAACACA 
TCCTATCGCT ACACCAACTC AGTATTGCAG AGGAAAAATG AGAAAAATCT CTTTCCAAGG 
CGAAAAACAC CTTGGGCAGC TGTAAATCAC AGCTCGGCCT CGTATTCTAA CTGGCCAGGG 
CACCTTGACT CACAGACAAC CACAGCTGTA GGAAGTGCTG CCAGAAGGGG TCTCCTGACC 
TCCCGCCATT CTTCCTCAGC TACAACCTCA GGGCAGCAAA AGCCCCTGGA GAAAACCATC 
AGCAGCCGAG CCAATTTAAG ACCGGTCACG CCAACCCACG GCTTTCTAAG AAACACTGAT 
GCTCAAATGA AAACACTCCC CCATAGATCC AAAGTCGAAG GCACGGGGGA CACCCACGCA 
CGGCGGGCTA CAGAGTCTGT TATTACCAGA GAGTCATACA GAGGTCACCA GGGCCATGTG 
GCAGCAGGTG CCGTGAGCAG CACTCCGTCA AATGAGAGGA CTGTCATTCT GGGAAAGAAA 
TTAGAAGCGC AAGCCACTAA AGAACAAGAA AGGGACAGAT CAGGGGTCAT CAGAATAAAG 
CCAGAAGAGA AAATGTTTGA TTCTAAAGAG AAGGCTTCAG AGGAAAGAAA CTTGAGGTGG 
GAAGAGCTAA CCAAGTTAGA TAGAGATGCC AGAAAGAGAG AAAGCCGGCA TTTGAGGGAT 
GAGGCCAGAG AGAAGGAAGC ACTGAAGGAG AGAAGTGTAA AAGAAAGAGA GGTGCCCATC 
AGTCTAGAAG TATCCCGGGG CAGCAGAGCA GAGGTGTCCA CCATACACTT GCAGTCACCT 
GGAAGGAAGG ACGTTAGCCA CAGTGGGGGA AGAGAGGCAG AGACCAAAGA GACAAGGTTC 
CGGCTGGATA CCCAGGACAC TGCCAGCTCT CTGCAAAGTG ACTCAACGAC AGAAACCATA 
GCCGAAAGCA TTGTCACCAC CATCCTTAAG CAGTTTACCC AGTCTCCAGG TGCCGAGGAG 
GAGGCCACTT CTTTCCCGGA CACAAAGGTC ACTTATGTGG ACAGGAAAGA GTTTCCTGGT 
GATGGGAAAA CCAAGACAGA AATCGTTGTG GAGTCGAAGC TGACAGATGT TGTTGATGTT 
TCTGATGAAG CAGGCCTGGA CTACCTTCTA AGCAAGGATG TCAAGGAAGT AGGACTGAAG 
GGGAAGTCAA CGGAGACGAT GATCGGAGAG ATGATCAACC TGGGTCTGAA AGGCAGGGAG 
GGGAGAGCTA AAGTCGTAAA TGTGGAGATT GTGGAAGAGC CCATGAGCTA CATAGGTGGT 
GGGAAAATAG ACTTTTCTAC CCCCTTTCAG GTAGAAGAAG TCGACGACGT GTCTCCCAGC 
CCCAAGGGCT TTGTGGAGGA AGAGGATGGT GAAGGAGAGA CCCACATGGC ATTCTCAATG 
CGTCCACATC AGACCAAACA GCCCCAGGGG ACCATCCCTC ACGTGGAAGA AGTGACGGAG 
GCGGGCGACT CAGAGGGGGA GCAGAGCTAT TTTGTGTCTA CCCCAGATGA GTACCCCGGG 
GGGCATGACA GAGAAGATGA TGGCTCAGTG TATGGGCAGA TTCATATCGA GGAAGAGTCT 
ACCATCCGGT ACTCATGGCA AGACGAAATT GCGCAGGGGA CCTGGAGAAG GAAAATGAGA 
GGTGACGTGG GGGGAGAGAA GCCTGTGAAG GTCCTGGAGG TCCCAGCACT TTCCCTGGGT 
GGTGCTATTG GTTCTGCTCA CTTGAAGGAA GAAGCCAGTG GTGAACTCCG TGCTGAACCC 
ACAGTCATTG AGAAAGAGAT CAAAATACCG CATGAATTCC ACACCTCCAT CAAGGGTGTC 
TTCTCCAGTG AGCCCCGGCA CCAGCTGGTG GAGGTCATCG GGCAGCTGGA GGAAACATTG 
CCCGAGCGCA TGAAGGAGGA GCTGTCTGCC CTGACCAGAC AGAGTCAAGG AGAGTCAGGG 
AGCGTTTCTG TGGACGTAAA GAAAGTCCAG AGCGCTGCTG GTGGTTCTGT GACTTTGATG 
GCTGAGGTCA ACCTCTCACA GACTGTAGAT GCTGATCAGT TGGACCTGGA GCAGTTGAGC 
AGAGATGAAG CTGGGGAAAT AGAGAGGGCT GTGGAGTCTG TGGTCAGAGA GAGCTTGGCC 
AAGCGCTCCA GCCCAGTGCC TAGAAGCCCA GACAGGGAAG ATGGAGAGGA GGTGCCTGCT 
GGTGGCATTC TCTTCAAGCG CTGGGCCACC AGGGAGCTGT ATAGCCCATC TGGTGAGAGG 
GATGATGCTG GCCAGGTCTC TCCCAGCTCA GATCAGCGTG TTACCCAGGG CCCAGTGTCA 
GCTACTGTGG AAGTGACCAG CCCAACGGGT TTTGTACAGT CTCATGTGCT GGAGGATGTG 
AGCCAGTCTG TAAGGCATGT TAAACTAGGT CCCACTGAAA TGTGGAGGAC AGAACAGGTG 
ACCTTCGGAG GACCCACCGC ACAGGTGGTG GAGATGGATC TGAGCGACAC GAGAGCGATC 
CGCAGCTGGA CCCGAGACAC AGGGTCGGAA GTGGAAGCTC ACGGCGTGTC TCACCGTGGT 
GGCTGGAGAA TTGCACACAG TAGGGATGAG CGAGTGGCCA GCACTGGCTC TGGGGCCTCC 
CCTGGGGATG CACACCAGGC CCCTGGAGAG AAAGGCACGG AGCAGGCTGG ATTTGACAAG 
ACGGTACAGT TGCAGAGAAT GGTAGACCAA AGGTCGGTGG CCTCAGATGA GAAGAAAGTG 
GCTCTTCTTT ATCTAGACAA TGAAGAGGAG GAGGAGGAGG AGGGCGAAGG GTGGTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Synm ( NM_183312.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_899135.3 Mus musculus synemin, intermediate filament protein (Synm), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu58365
Clone ID Related Accession (Same CDS sequence)	NM_183312.3
Accession Version	NM_183312.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Mus musculus(house mouse)
Product	synemin isoform L
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143019.1 and AC158296.6. On Jul 25, 2007 this sequence version replaced NM_183312.2. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region that results in a frameshift and early stop codon, compared to variant 1. The encoded protein (isoform L) has a shorter and distinct C-terminus, compared to isoform H. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ579702.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTGTCCT GGCGGCTGCA GACGGGCTCT GAAAAGGCGG AGCTGCAGGA GCTCAACGCC 
CGGCTTTACG ACTACGTGTG CCGGGTGCGA GAGCTGGAGC GCGAGAACCT GCTACTGGAG 
GAGGAGTTGC GCAGCCGGCT GAGCCGGGAG GACCGCTGGG CTGAGGATCA GGCACTCTAC 
GCCGAGGAGG CGCGCAGCCT ACGACAGCAG CTAGATGAAC TGAACTGGTC CACAGCCCTG 
GCCGAGGGCG AGCGCGACGC GCTGCGGAGG GAACTGCTGG AGCTGCAGCG GGAGGGTGTG 
GAAGCCGGCA CTGCCCGCAG CCGCCTGGAT GCCGAGCTGG GAGCGCAACG GCGGGAGCTA 
GAGGAGGCTC TGGGCGCGCG CGCCGCCCTC GAGGCGCTGC TGGGCCGGCT GGAAACTGAG 
CGCCGCGACT TGGACGCTGC CCACGAACGC CAAGTGCGAG ATCTACGAGC CCGCGCTGCC 
AGCCTCACCA TGCACTTCCG AGCCCGCGCC ACCAGCCCCG CCGCGCCCCC GCCGCGCCTG 
CGGGACGTGC ACGACAGCTA CGCCCTGCTG GTGGCCGAGT CGTGGAGGGA GAGCGTGCAG 
CTGTACGAGG ACGAGGTGCG CGAGCTGGAG CAGGCGCTGC GCCGCGGCCA AGAGAGCCGG 
CTCCAGGCGG AGGACGAGGC GCGGCTGTGC GCGCAGGAGG CAGACGCGCT GCGGAACCAG 
GCGCTGGAGT TGGAGCAGCT GCGCGCGAGG CTGGAGGATG AGCTGCTGCG GATGCGCGAG 
GAGTACGGGA TGCAGGCGGA GGAGCGGCAG AGAGTGATTG ACAGCCTGGA GGATGAGAAG 
GAAGCCCTTA CCTTGGCCAT GGCTGACCGG CTGCGGGATT ACCAGGAGCT CCTGCAGGTG 
AAGACTGGAC TCAGCTTGGA GGTAGCCACC TACAGGGCCT TGTTGGAAGG AGAAAGCAAT 
CCGGAGATAT TGATCTGGAC TGAGAACATT GAAAACGTGC CACAAGATGG ATCTGAGCGA 
CACGAGAGCG ATCCGCAGCT GGACCCGAGA CACAGGGTCG GAAGTGGAAG CTCACGGCGT 
GTCTCACCGT GGTGGCTGGA GAATTGCACA CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899135.3
CDS	115..1230
Translation	MLSWRLQTGSEKAELQELNARLYDYVCRVRELERENLLLEEELRSRLSREDRWAEDQALYAEEARSLRQQLDELNWSTALAEGERDALRRELLELQREGVEAGTARSRLDAELGAQRRELEEALGARAALEALLGRLETERRDLDAAHERQVRDLRARAASLTMHFRARATSPAAPPPRLRDVHDSYALLVAESWRESVQLYEDEVRELEQALRRGQESRLQAEDEARLCAQEADALRNQALELEQLRARLEDELLRMREEYGMQAEERQRVIDSLEDEKEALTLAMADRLRDYQELLQVKTGLSLEVATYRALLEGESNPEILIWTENIENVPQDGSERHESDPQLDPRHRVGSGSSRRVSPWWLENCTQ

Target ORF information:

RefSeq Version	NM_183312.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synemin, intermediate filament protein (Synm), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183312.3

ORF Insert Sequence:

ATGCTGTCCT GGCGGCTGCA GACGGGCTCT GAAAAGGCGG AGCTGCAGGA GCTCAACGCC 
CGGCTTTACG ACTACGTGTG CCGGGTGCGA GAGCTGGAGC GCGAGAACCT GCTACTGGAG 
GAGGAGTTGC GCAGCCGGCT GAGCCGGGAG GACCGCTGGG CTGAGGATCA GGCACTCTAC 
GCCGAGGAGG CGCGCAGCCT ACGACAGCAG CTAGATGAAC TGAACTGGTC CACAGCCCTG 
GCCGAGGGCG AGCGCGACGC GCTGCGGAGG GAACTGCTGG AGCTGCAGCG GGAGGGTGTG 
GAAGCCGGCA CTGCCCGCAG CCGCCTGGAT GCCGAGCTGG GAGCGCAACG GCGGGAGCTA 
GAGGAGGCTC TGGGCGCGCG CGCCGCCCTC GAGGCGCTGC TGGGCCGGCT GGAAACTGAG 
CGCCGCGACT TGGACGCTGC CCACGAACGC CAAGTGCGAG ATCTACGAGC CCGCGCTGCC 
AGCCTCACCA TGCACTTCCG AGCCCGCGCC ACCAGCCCCG CCGCGCCCCC GCCGCGCCTG 
CGGGACGTGC ACGACAGCTA CGCCCTGCTG GTGGCCGAGT CGTGGAGGGA GAGCGTGCAG 
CTGTACGAGG ACGAGGTGCG CGAGCTGGAG CAGGCGCTGC GCCGCGGCCA AGAGAGCCGG 
CTCCAGGCGG AGGACGAGGC GCGGCTGTGC GCGCAGGAGG CAGACGCGCT GCGGAACCAG 
GCGCTGGAGT TGGAGCAGCT GCGCGCGAGG CTGGAGGATG AGCTGCTGCG GATGCGCGAG 
GAGTACGGGA TGCAGGCGGA GGAGCGGCAG AGAGTGATTG ACAGCCTGGA GGATGAGAAG 
GAAGCCCTTA CCTTGGCCAT GGCTGACCGG CTGCGGGATT ACCAGGAGCT CCTGCAGGTG 
AAGACTGGAC TCAGCTTGGA GGTAGCCACC TACAGGGCCT TGTTGGAAGG AGAAAGCAAT 
CCGGAGATAT TGATCTGGAC TGAGAACATT GAAAACGTGC CACAAGATGG ATCTGAGCGA 
CACGAGAGCG ATCCGCAGCT GGACCCGAGA CACAGGGTCG GAAGTGGAAG CTCACGGCGT 
GTCTCACCGT GGTGGCTGGA GAATTGCACA CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Absence of synemin in mice causes structural and functional abnormalities in heart.
Journal of molecular and cellular cardiology114354-363(2017 Dec)
Garc?a-Pelagio KP,Chen L,Joca HC,Ward C,Jonathan Lederer W,Bloch RJ

Vimentin knockout results in increased expression of sub-endothelial basement membrane components and carotid stiffness in mice.
Scientific reports7(1)11628(2017 Sep)
Langlois B,Belozertseva E,Parlakian A,Bourhim M,Gao-Li J,Blanc J,Tian L,Coletti D,Labat C,Ramdame-Cherif Z,Challande P,Regnault V,Lacolley P,Zhenlin L

Deficiency of the intermediate filament synemin reduces bone mass in vivo.
American journal of physiology. Cell physiology311(6)C839-C845(2016 Dec)
Moorer MC,Buo AM,Garcia-Pelagio KP,Stains JP,Bloch RJ

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Myopathic changes in murine skeletal muscle lacking synemin.
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Synm cDNA ORF clone, Mus musculus(house mouse)

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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