synm cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	synm
Entrez Gene ID	558800
Full Name	synemin, intermediate filament protein
Synonyms	dmn,si:dkey-25f3.3
General protein information	Preferred Names synemin, intermediate filament protein Names synemin desmuslin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021467662.1	XP_021323337.1	synemin isoform X1
NM_001044875.2	NP_001038340.1	synemin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SYNM	XP_003314921.1
	SYNM	XP_001108469.2
Homo sapiens (human)	SYNM	NP_663780.2
Bos taurus (cattle)	SYNM	NP_001178382.1
Danio rerio (zebrafish)	synm	XP_005159213.1
Rattus norvegicus (Norway rat)	Synm	NP_001128330.1
Xenopus tropicalis (tropical clawed frog)	synm	XP_002939315.2
Mus musculus (house mouse)	Synm	NP_964001.2
Gallus gallus (chicken)	SYNM	NP_990140.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Extracellular component hyaluronic acid and its receptor Hmmr are required for epicardial EMT during heart regeneration.
Missinato MA, Tobita K, Romano N, Carroll JA, Tsang M
Cardiovascular research107(4)487-98(2015 Sep)

Consequences of lineage-specific gene loss on functional evolution of surviving paralogs: ALDH1A and retinoic acid signaling in vertebrate genomes.
Ca?estro C, Catchen JM, Rodr?guez-Mar? A, Yokoi H, Postlethwait JH
PLoS genetics5(5)e1000496(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following synm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the synm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa149865	XM_021467662.1 Latest version!	Danio rerio synemin, intermediate filament protein (synm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa23336	NM_001044875.2 Latest version!	Danio rerio synemin, intermediate filament protein (synm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

synm ( XM_021467662.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323337.1 Danio rerio synemin, intermediate filament protein (synm), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa149865
Clone ID Related Accession (Same CDS sequence)	XM_021467662.1
Accession Version	XM_021467662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synemin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTA ATCAGCATTC AAGAGAAAGA ATTATAGATA TTAAAACACC AGCCCATGTT 
TACACTCCAA GAGTCTCTGT TAATTCTGCA AGTCGGCCGG ATACAAGAAA AAAAGCCTTC 
ACAGGATATG ATGTTAAATA TATGGAGCCT GTTTCCAGCA TGAGATCCTC ATCAACATCC 
AGTCAGTTTC ATTCTTACGA ACCCTCTAGG ATTGTGCCCA TCAGTGTATC AAATCGTGCC 
CAGCAGAGTC CTGATTCTAG AAGGGACATG ATTTCATTCA CCAAAGCAGC TCAAGCAGCT 
GCTTCCGGTA CCTCAAAACC TGGAGTTTCT TCTGTAGAGG TAAAAAGAGA GGAGATAGAT 
CAGAGATATC TGAGAAAAGA GGTCTCTCAG CACCCTTCAA AAAGCTCACC TGATCTTCCC 
CCCACTAGAA TCGAAAGTAC ACCAAGCTCA ACCATGTCAA AAATAGAGAA ACCAAAGTCA 
GTGAATGTAA TATCACCTAC TGTGAGCTTG AGCAAGACCA TCACAGAAGA AAGCCGAGAG 
AAAGATGTTA AACTGAAAGA AAAGAAAATG GATTTCAAAC TAGATCAAGA CAAATCTGAG 
GCATCAATAA AAGATAAAAG AGGGGATGTG AAAGAATCAA TCGCCAAAGA TGTGAGAGAG 
TCTGAACATG TGGGGCATAA GGTATTTGTA GGTGAGGAGA AAATGTTAGA TGCTGTTTCT 
ATGGAGGAAA TCATTCAGAA GGTCATGAAA CCAGCCGGTT TAGATCCTAA GGTCAGCTCA 
TCCCCTGACT CAAAGGTCAC ATATCACGTA GAGAAAACAG AACAAGAGGA TGGAACCACC 
AAGACTCAGA TTGTCTTACA ATCGAAAGTT GAGGAGGAGC TGGACCTGTC TGAAGATTCT 
GCCCTAGAGG AGCTTCTCAG CCAGGGAGTC AAGAAGGTCA CTCTGGAGAA CATCAAAGGA 
ACCCCAACGG GAGCCATGAT TGAGAACCTG TTGAGTCTTG GCCTGCAAGG TGAGAGTTTG 
GAAAATAAGT TGGTGAATGT GGAGGTAATC GAGGAACCGG TGGATTCTCA GAGTGATGAG 
GAGAGTGAAA TTGAAATAGA GGAGACTGTG GAGATTAAGT CTAAACAGCC AAGCATCAGT 
CCCTCATCAA TGTCCTTCCA AATCGAGGAG CTAGAGAGTG ACCCTAAGAC CACAAAACCC 
TACGAGACTG ATTTTGCTAA ATCCTCACGT TATGGGAACA GTGGGTCTAT GCAGTTTCAG 
GATGTTTCCA AAGAAGAAAG TCTACCCTAC TTCTCACATG GTAAAGACTC ACAAGAATAC 
TTCGTCTCCA CTCCTGACGA GAACCTGTCA GAGTCCGAGG AAAAAGACAA TGAGTCGGAG 
GATGGCGGAA GGTTTATGTC ATATAGCCAT TACGGAGTTG TGGATGATCT GTCTGATGAA 
AGATATTACC AGGAAGATGA TCCAAAACGA CCCACTGCTG AAGGTCACAG TTACAGAGAC 
TCACCTGATT ATGGTGACCG CTCGTTTCTG AGAGACAATA TCCAAGACTG CATAATTGAA 
GAGGAGGTGC ACGTTTCTCC CACTCTGCAA CAGTCCATAG TGGGGATCCT GAGAGAGGAC 
TCACTAGACC CCGAACAGCA GCTCAGAGGA GCATTAGAGC AAATCCAAGA CACTGTTTCT 
GGAGCCCTCA AGGAAGAGCT GGCATTTTTC ACGAAAGGTA GAGAGACTCC AGAGAATGTC 
TCGGTGGACA TCAAAAAAAT AGAACAAGTT GCAGACAATG GAACCATGAC TATTGTGGCG 
GAGCTCAATG TGTCCCAGAC GTTGGAGGAG TCTGGACTGC TGGAAGAAGC AGAAGATGAT 
CCATCTGAAG AGCAGATCAT GGCGGCGCTG AGCTCATCTC ATCCAATGCT CCAGCAGGCC 
ATAGGTGGAG CTGCCGGTAC AGGATACACC ATGAAAATTT CCAAAGAGGA GTTTGAAACG 
GACGAAGTGC CATGGATGAC CACCGATGAA GAGTTCCAAC AGTGGAGCTC AACCGATGGG 
TTTGGCAAGA CAGAAAAACA CATCAAGCTC GGCCCTACTG AGAGATCTTT TACTTTTCAG 
ATGGATGTGA ACAACGGCTC TACTGCATCA GTGAGTGAAG GAGCCATCGA AGTGCAAAGC 
AGCTCTTCTG AATTCTCACA GACTCAGATG ATTGAGCCAC ACTTAAAGGT CTGTCATGAG 
AAAAGAGTCG CAACTGTTTA TCTTGAAAGC CCCAAAGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323337.1
CDS	288..2552
Translation	MWSNQHSRERIIDIKTPAHVYTPRVSVNSASRPDTRKKAFTGYDVKYMEPVSSMRSSSTSSQFHSYEPSRIVPISVSNRAQQSPDSRRDMISFTKAAQAAASGTSKPGVSSVEVKREEIDQRYLRKEVSQHPSKSSPDLPPTRIESTPSSTMSKIEKPKSVNVISPTVSLSKTITEESREKDVKLKEKKMDFKLDQDKSEASIKDKRGDVKESIAKDVRESEHVGHKVFVGEEKMLDAVSMEEIIQKVMKPAGLDPKVSSSPDSKVTYHVEKTEQEDGTTKTQIVLQSKVEEELDLSEDSALEELLSQGVKKVTLENIKGTPTGAMIENLLSLGLQGESLENKLVNVEVIEEPVDSQSDEESEIEIEETVEIKSKQPSISPSSMSFQIEELESDPKTTKPYETDFAKSSRYGNSGSMQFQDVSKEESLPYFSHGKDSQEYFVSTPDENLSESEEKDNESEDGGRFMSYSHYGVVDDLSDERYYQEDDPKRPTAEGHSYRDSPDYGDRSFLRDNIQDCIIEEEVHVSPTLQQSIVGILREDSLDPEQQLRGALEQIQDTVSGALKEELAFFTKGRETPENVSVDIKKIEQVADNGTMTIVAELNVSQTLEESGLLEEAEDDPSEEQIMAALSSSHPMLQQAIGGAAGTGYTMKISKEEFETDEVPWMTTDEEFQQWSSTDGFGKTEKHIKLGPTERSFTFQMDVNNGSTASVSEGAIEVQSSSSEFSQTQMIEPHLKVCHEKRVATVYLESPKDE

Target ORF information:

RefSeq Version	XM_021467662.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synemin, intermediate filament protein (synm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467662.1

ORF Insert Sequence:

ATGTGGTCTA ATCAGCATTC AAGAGAAAGA ATTATAGATA TTAAAACACC AGCCCATGTT 
TACACTCCAA GAGTCTCTGT TAATTCTGCA AGTCGGCCGG ATACAAGAAA AAAAGCCTTC 
ACAGGATATG ATGTTAAATA TATGGAGCCT GTTTCCAGCA TGAGATCCTC ATCAACATCC 
AGTCAGTTTC ATTCTTACGA ACCCTCTAGG ATTGTGCCCA TCAGTGTATC AAATCGTGCC 
CAGCAGAGTC CTGATTCTAG AAGGGACATG ATTTCATTCA CCAAAGCAGC TCAAGCAGCT 
GCTTCCGGTA CCTCAAAACC TGGAGTTTCT TCTGTAGAGG TAAAAAGAGA GGAGATAGAT 
CAGAGATATC TGAGAAAAGA GGTCTCTCAG CACCCTTCAA AAAGCTCACC TGATCTTCCC 
CCCACTAGAA TCGAAAGTAC ACCAAGCTCA ACCATGTCAA AAATAGAGAA ACCAAAGTCA 
GTGAATGTAA TATCACCTAC TGTGAGCTTG AGCAAGACCA TCACAGAAGA AAGCCGAGAG 
AAAGATGTTA AACTGAAAGA AAAGAAAATG GATTTCAAAC TAGATCAAGA CAAATCTGAG 
GCATCAATAA AAGATAAAAG AGGGGATGTG AAAGAATCAA TCGCCAAAGA TGTGAGAGAG 
TCTGAACATG TGGGGCATAA GGTATTTGTA GGTGAGGAGA AAATGTTAGA TGCTGTTTCT 
ATGGAGGAAA TCATTCAGAA GGTCATGAAA CCAGCCGGTT TAGATCCTAA GGTCAGCTCA 
TCCCCTGACT CAAAGGTCAC ATATCACGTA GAGAAAACAG AACAAGAGGA TGGAACCACC 
AAGACTCAGA TTGTCTTACA ATCGAAAGTT GAGGAGGAGC TGGACCTGTC TGAAGATTCT 
GCCCTAGAGG AGCTTCTCAG CCAGGGAGTC AAGAAGGTCA CTCTGGAGAA CATCAAAGGA 
ACCCCAACGG GAGCCATGAT TGAGAACCTG TTGAGTCTTG GCCTGCAAGG TGAGAGTTTG 
GAAAATAAGT TGGTGAATGT GGAGGTAATC GAGGAACCGG TGGATTCTCA GAGTGATGAG 
GAGAGTGAAA TTGAAATAGA GGAGACTGTG GAGATTAAGT CTAAACAGCC AAGCATCAGT 
CCCTCATCAA TGTCCTTCCA AATCGAGGAG CTAGAGAGTG ACCCTAAGAC CACAAAACCC 
TACGAGACTG ATTTTGCTAA ATCCTCACGT TATGGGAACA GTGGGTCTAT GCAGTTTCAG 
GATGTTTCCA AAGAAGAAAG TCTACCCTAC TTCTCACATG GTAAAGACTC ACAAGAATAC 
TTCGTCTCCA CTCCTGACGA GAACCTGTCA GAGTCCGAGG AAAAAGACAA TGAGTCGGAG 
GATGGCGGAA GGTTTATGTC ATATAGCCAT TACGGAGTTG TGGATGATCT GTCTGATGAA 
AGATATTACC AGGAAGATGA TCCAAAACGA CCCACTGCTG AAGGTCACAG TTACAGAGAC 
TCACCTGATT ATGGTGACCG CTCGTTTCTG AGAGACAATA TCCAAGACTG CATAATTGAA 
GAGGAGGTGC ACGTTTCTCC CACTCTGCAA CAGTCCATAG TGGGGATCCT GAGAGAGGAC 
TCACTAGACC CCGAACAGCA GCTCAGAGGA GCATTAGAGC AAATCCAAGA CACTGTTTCT 
GGAGCCCTCA AGGAAGAGCT GGCATTTTTC ACGAAAGGTA GAGAGACTCC AGAGAATGTC 
TCGGTGGACA TCAAAAAAAT AGAACAAGTT GCAGACAATG GAACCATGAC TATTGTGGCG 
GAGCTCAATG TGTCCCAGAC GTTGGAGGAG TCTGGACTGC TGGAAGAAGC AGAAGATGAT 
CCATCTGAAG AGCAGATCAT GGCGGCGCTG AGCTCATCTC ATCCAATGCT CCAGCAGGCC 
ATAGGTGGAG CTGCCGGTAC AGGATACACC ATGAAAATTT CCAAAGAGGA GTTTGAAACG 
GACGAAGTGC CATGGATGAC CACCGATGAA GAGTTCCAAC AGTGGAGCTC AACCGATGGG 
TTTGGCAAGA CAGAAAAACA CATCAAGCTC GGCCCTACTG AGAGATCTTT TACTTTTCAG 
ATGGATGTGA ACAACGGCTC TACTGCATCA GTGAGTGAAG GAGCCATCGA AGTGCAAAGC 
AGCTCTTCTG AATTCTCACA GACTCAGATG ATTGAGCCAC ACTTAAAGGT CTGTCATGAG 
AAAAGAGTCG CAACTGTTTA TCTTGAAAGC CCCAAAGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

synm ( NM_001044875.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038340.1 Danio rerio synemin, intermediate filament protein (synm), mRNA.

Price & Availability↑

CloneID	ODa23336
Clone ID Related Accession (Same CDS sequence)	NM_001044875.2
Accession Version	NM_001044875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	synemin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX470160.9. On or before Oct 24, 2015 this sequence version replaced XM_005159156.2, NM_001044875.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC171396.1, EB926821.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTTTCGAA TGAGGGAACC GTTTGAAAGC GAGAAGCTCC AGCTGCAAGA GCTCAACCAA 
AGACTCAGCC ACTACCTAAT GCGCTCGAAA CAGTTGGAAC AAGAAAACAC CTGTCTGATT 
AACGAAATAA GCTCAATCAG GCAGAACAGG TCTGGAGAAT GGGAAAGCAA GCACATGTCC 
GAGCTGCGAG AAATGAGGAG ACTGGTGGAG CGTTTATCTT TCGAGAAATG CAAAGCGGAG 
ATGGAGCGAC AGAAGCTGCG CAATGAACTT CAGGCGCTGC AGGCGATGCG TTCAGATGAA 
GCCTCGGTCA GCAAAAGCAT CGGGACTGAG CTTAAAGGCT GCGAGAGGCA ACTTCAGAAC 
GCTATTCAGG GCAACGGTGC TTTGGAGAGT CGTCTCTTTG AGCTGGAAAC GGAATACAAG 
TTTTTGGAAG ATGCCCACAG AAAGGAAGTG GCCCACCTGA GGGACCAGGT GCGCTCCAGA 
ACTGTGCGAG TCGTGACTCA AACGCGTCCC GCTCCTCCAG CGGTTACTAT GGAGGAGGTG 
CAGGAGTACG CAAACAACTT CACCGAAAAC TGGCAGGGCA CCTTGGAGAT GTACCGCCTG 
CAGGTGGAGG AAATCGAAGA GTCGATAAAA GCAGACCACG CGAGGCTGGA GGAGATTCAG 
AGGGAAAAGA AGGAACACGC TTCGCAGTTC AAGAGGCTGC GTGACGAGAT TGAGAAACAA 
ACACAAGTGC AGATGAATCT CGAGGAGCAG CTCGTGAACA TGCAAGATCA GTTCAGAGCT 
GAAATCACTC AGTATCAGGC TATTATTGAG GAACTAGAGT ATGAGCGCAG AATGCTGTCC 
AGCAACATCT CAGATAAGCT GAAGGACCAT CAAGATCTAT TACAGGTCAA GATGGGTCTC 
GCTCTGGAAG TGGCAGCATA CAGGGCGCTC TTGGAAGAAG AAGGAAGACA TGCTAAAATG 
TGGTCTAATC AGCATTCAAG AGAAAGAATT ATAGATATTA AAACACCAGC CCATGTTTAC 
ACTCCAAGAG TCTCTGTTAA TTCTGCAAGT CGGCCGGATA CAAGAAAAAA AGCCTTCACA 
GGATATGATG TTAAATATAT GGAGCCTGTT TCCAGCATGA GATCCTCATC AACATCCAGT 
CAGTTTCATT CTTACGAACC CTCTAGGATT GTGCCCATCA GTGTATCAAA TCGTGCCCAG 
CAGAGTCCTG ATTCTAGAAG GGACATGATT TCATTCACCA AAGCAGCTCA AGCAGCTGCT 
TCCGGTACCT CAAAACCTGG AGTTTCTTCT GTAGAGGTAA AAAGAGAGGA GATAGATCAG 
AGATATCTGA GAAAAGAGGT CTCTCAGCAC CCTTCAAAAA GCTCACCTGA TCTTCCCCCC 
ACTAGAATCG AAAGTACACC AAGCTCAACC ATGTCAAAAA TAGAGAAACC AAAGTCAGTG 
AATGTAATAT CACCTACTGT GAGCTTGAGC AAGACCATCA CAGAAGAAAG CCGAGAGAAA 
GATGTTAAAC TGAAAGAAAA GAAAATGGAT TTCAAACTAG ATCAAGACAA ATCTGAGGCA 
TCAATAAAAG ATAAAAGAGG GGATGTGAAA GAATCAATCG CCAAAGATGT GAGAGAGTCT 
GAACATGTGG GGCATAAGGT ATTTGTAGGT GAGGAGAAAA TGTTAGATGC TGTTTCTATG 
GAGGAAATCA TTCAGAAGGT CATGAAACCA GCCGGTTTAG ATCCTAAGGT CAGCTCATCC 
CCTGACTCAA AGGTCACATA TCACGTAGAG AAAACAGAAC AAGAGGATGG AACCACCAAG 
ACTCAGATTG TCTTACAATC GAAAGTTGAG GAGGAGCTGG ACCTGTCTGA AGATTCTGCC 
CTAGAGGAGC TTCTCAGCCA GGGAGTCAAG AAGGTCACTC TGGAGAACAT CAAAGGAACC 
CCAACGGGAG CCATGATTGA GAACCTGTTG AGTCTTGGCC TGCAAGGTGA GAGTTTGGAA 
AATAAGTTGG TGAATGTGGA GGTAATCGAG GAACCGGTGG ATTCTCAGAG TGATGAGGAG 
AGTGAAATTG AAATAGAGGA GACTGTGGAG ATTAAGTCTA AACAGCCAAG CATCAGTCCC 
TCATCAATGT CCTTCCAAAT CGAGGAGCTA GAGAGTGACC CTAAGACCAC AAAACCCTAC 
GAGACTGATT TTGCTAAATC CTCACGTTAT GGGAACAGTG GGTCTATGCA GTTTCAGGAT 
GTTTCCAAAG AAGAAAGTCT ACCCTACTTC TCACATGGTA AAGACTCACA AGAATACTTC 
GTCTCCACTC CTGACGAGAA CCTGTCAGAG TCCGAGGAAA AAGACAATGA GTCGGAGGAT 
GGCGGAAGGT TTATGTCATA TAGCCATTAC GGAGTTGTGG ATGATCTGTC TGATGAAAGA 
TATTACCAGG AAGATGATCC AAAACGACCC ACTGCTGAAG GTCACAGTTA CAGAGACTCA 
CCTGATTATG GTGACCGCTC GTTTCTGAGA GACAATATCC AAGACTGCAT AATTGAAGAG 
GAGGTGCACG TTTCTCCCAC TCTGCAACAG TCCATAGTGG GGATCCTGAG AGAGGACTCA 
CTAGACCCCG AACAGCAGCT CAGAGGAGCA TTAGAGCAAA TCCAAGACAC TGTTTCTGGA 
GCCCTCAAGG AAGAGCTGGC ATTTTTCACG AAAGGTAGAG AGACTCCAGA GAATGTCTCG 
GTGGACATCA AAAAAATAGA ACAAGTTGCA GACAATGGAA CCATGACTAT TGTGGCGGAG 
CTCAATGTGT CCCAGACGTT GGAGGAGTCT GGACTGCTGG AAGAAGCAGA AGATGATCCA 
TCTGAAGAGC AGATCATGGC GGCGCTGAGC TCATCTCATC CAATGCTCCA GCAGGCCATA 
GGTGGAGCTG CCGGTACAGG ATACACCATG AAAATTTCCA AAGAGGAGTT TGAAACGGAC 
GAAGTGCCAT GGATGACCAC CGATGAAGAG TTCCAACAGT GGAGCTCAAC CGATGGGTTT 
GGCAAGACAG AAAAACACAT CAAGCTCGGC CCTACTGAGA GATCTTTTAC TTTTCAGATG 
GATGTGAACA ACGGCTCTAC TGCATCAGTG AGTGAAGGAG CCATCGAAGT GCAAAGCAGC 
TCTTCTGAAT TCTCACAGAC TCAGATGATT GAGCCACACT TAAAGGTCTG TCATGAGAAA 
AGAGTCGCAA CTGTTTATCT TGAAAGCCCC AAAGATGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038340.1
CDS	93..3314
Translation	MFRMREPFESEKLQLQELNQRLSHYLMRSKQLEQENTCLINEISSIRQNRSGEWESKHMSELREMRRLVERLSFEKCKAEMERQKLRNELQALQAMRSDEASVSKSIGTELKGCERQLQNAIQGNGALESRLFELETEYKFLEDAHRKEVAHLRDQVRSRTVRVVTQTRPAPPAVTMEEVQEYANNFTENWQGTLEMYRLQVEEIEESIKADHARLEEIQREKKEHASQFKRLRDEIEKQTQVQMNLEEQLVNMQDQFRAEITQYQAIIEELEYERRMLSSNISDKLKDHQDLLQVKMGLALEVAAYRALLEEEGRHAKMWSNQHSRERIIDIKTPAHVYTPRVSVNSASRPDTRKKAFTGYDVKYMEPVSSMRSSSTSSQFHSYEPSRIVPISVSNRAQQSPDSRRDMISFTKAAQAAASGTSKPGVSSVEVKREEIDQRYLRKEVSQHPSKSSPDLPPTRIESTPSSTMSKIEKPKSVNVISPTVSLSKTITEESREKDVKLKEKKMDFKLDQDKSEASIKDKRGDVKESIAKDVRESEHVGHKVFVGEEKMLDAVSMEEIIQKVMKPAGLDPKVSSSPDSKVTYHVEKTEQEDGTTKTQIVLQSKVEEELDLSEDSALEELLSQGVKKVTLENIKGTPTGAMIENLLSLGLQGESLENKLVNVEVIEEPVDSQSDEESEIEIEETVEIKSKQPSISPSSMSFQIEELESDPKTTKPYETDFAKSSRYGNSGSMQFQDVSKEESLPYFSHGKDSQEYFVSTPDENLSESEEKDNESEDGGRFMSYSHYGVVDDLSDERYYQEDDPKRPTAEGHSYRDSPDYGDRSFLRDNIQDCIIEEEVHVSPTLQQSIVGILREDSLDPEQQLRGALEQIQDTVSGALKEELAFFTKGRETPENVSVDIKKIEQVADNGTMTIVAELNVSQTLEESGLLEEAEDDPSEEQIMAALSSSHPMLQQAIGGAAGTGYTMKISKEEFETDEVPWMTTDEEFQQWSSTDGFGKTEKHIKLGPTERSFTFQMDVNNGSTASVSEGAIEVQSSSSEFSQTQMIEPHLKVCHEKRVATVYLESPKDE

Target ORF information:

RefSeq Version	NM_001044875.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio synemin, intermediate filament protein (synm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044875.2

ORF Insert Sequence:

ATGTTTCGAA TGAGGGAACC GTTTGAAAGC GAGAAGCTCC AGCTGCAAGA GCTCAACCAA 
AGACTCAGCC ACTACCTAAT GCGCTCGAAA CAGTTGGAAC AAGAAAACAC CTGTCTGATT 
AACGAAATAA GCTCAATCAG GCAGAACAGG TCTGGAGAAT GGGAAAGCAA GCACATGTCC 
GAGCTGCGAG AAATGAGGAG ACTGGTGGAG CGTTTATCTT TCGAGAAATG CAAAGCGGAG 
ATGGAGCGAC AGAAGCTGCG CAATGAACTT CAGGCGCTGC AGGCGATGCG TTCAGATGAA 
GCCTCGGTCA GCAAAAGCAT CGGGACTGAG CTTAAAGGCT GCGAGAGGCA ACTTCAGAAC 
GCTATTCAGG GCAACGGTGC TTTGGAGAGT CGTCTCTTTG AGCTGGAAAC GGAATACAAG 
TTTTTGGAAG ATGCCCACAG AAAGGAAGTG GCCCACCTGA GGGACCAGGT GCGCTCCAGA 
ACTGTGCGAG TCGTGACTCA AACGCGTCCC GCTCCTCCAG CGGTTACTAT GGAGGAGGTG 
CAGGAGTACG CAAACAACTT CACCGAAAAC TGGCAGGGCA CCTTGGAGAT GTACCGCCTG 
CAGGTGGAGG AAATCGAAGA GTCGATAAAA GCAGACCACG CGAGGCTGGA GGAGATTCAG 
AGGGAAAAGA AGGAACACGC TTCGCAGTTC AAGAGGCTGC GTGACGAGAT TGAGAAACAA 
ACACAAGTGC AGATGAATCT CGAGGAGCAG CTCGTGAACA TGCAAGATCA GTTCAGAGCT 
GAAATCACTC AGTATCAGGC TATTATTGAG GAACTAGAGT ATGAGCGCAG AATGCTGTCC 
AGCAACATCT CAGATAAGCT GAAGGACCAT CAAGATCTAT TACAGGTCAA GATGGGTCTC 
GCTCTGGAAG TGGCAGCATA CAGGGCGCTC TTGGAAGAAG AAGGAAGACA TGCTAAAATG 
TGGTCTAATC AGCATTCAAG AGAAAGAATT ATAGATATTA AAACACCAGC CCATGTTTAC 
ACTCCAAGAG TCTCTGTTAA TTCTGCAAGT CGGCCGGATA CAAGAAAAAA AGCCTTCACA 
GGATATGATG TTAAATATAT GGAGCCTGTT TCCAGCATGA GATCCTCATC AACATCCAGT 
CAGTTTCATT CTTACGAACC CTCTAGGATT GTGCCCATCA GTGTATCAAA TCGTGCCCAG 
CAGAGTCCTG ATTCTAGAAG GGACATGATT TCATTCACCA AAGCAGCTCA AGCAGCTGCT 
TCCGGTACCT CAAAACCTGG AGTTTCTTCT GTAGAGGTAA AAAGAGAGGA GATAGATCAG 
AGATATCTGA GAAAAGAGGT CTCTCAGCAC CCTTCAAAAA GCTCACCTGA TCTTCCCCCC 
ACTAGAATCG AAAGTACACC AAGCTCAACC ATGTCAAAAA TAGAGAAACC AAAGTCAGTG 
AATGTAATAT CACCTACTGT GAGCTTGAGC AAGACCATCA CAGAAGAAAG CCGAGAGAAA 
GATGTTAAAC TGAAAGAAAA GAAAATGGAT TTCAAACTAG ATCAAGACAA ATCTGAGGCA 
TCAATAAAAG ATAAAAGAGG GGATGTGAAA GAATCAATCG CCAAAGATGT GAGAGAGTCT 
GAACATGTGG GGCATAAGGT ATTTGTAGGT GAGGAGAAAA TGTTAGATGC TGTTTCTATG 
GAGGAAATCA TTCAGAAGGT CATGAAACCA GCCGGTTTAG ATCCTAAGGT CAGCTCATCC 
CCTGACTCAA AGGTCACATA TCACGTAGAG AAAACAGAAC AAGAGGATGG AACCACCAAG 
ACTCAGATTG TCTTACAATC GAAAGTTGAG GAGGAGCTGG ACCTGTCTGA AGATTCTGCC 
CTAGAGGAGC TTCTCAGCCA GGGAGTCAAG AAGGTCACTC TGGAGAACAT CAAAGGAACC 
CCAACGGGAG CCATGATTGA GAACCTGTTG AGTCTTGGCC TGCAAGGTGA GAGTTTGGAA 
AATAAGTTGG TGAATGTGGA GGTAATCGAG GAACCGGTGG ATTCTCAGAG TGATGAGGAG 
AGTGAAATTG AAATAGAGGA GACTGTGGAG ATTAAGTCTA AACAGCCAAG CATCAGTCCC 
TCATCAATGT CCTTCCAAAT CGAGGAGCTA GAGAGTGACC CTAAGACCAC AAAACCCTAC 
GAGACTGATT TTGCTAAATC CTCACGTTAT GGGAACAGTG GGTCTATGCA GTTTCAGGAT 
GTTTCCAAAG AAGAAAGTCT ACCCTACTTC TCACATGGTA AAGACTCACA AGAATACTTC 
GTCTCCACTC CTGACGAGAA CCTGTCAGAG TCCGAGGAAA AAGACAATGA GTCGGAGGAT 
GGCGGAAGGT TTATGTCATA TAGCCATTAC GGAGTTGTGG ATGATCTGTC TGATGAAAGA 
TATTACCAGG AAGATGATCC AAAACGACCC ACTGCTGAAG GTCACAGTTA CAGAGACTCA 
CCTGATTATG GTGACCGCTC GTTTCTGAGA GACAATATCC AAGACTGCAT AATTGAAGAG 
GAGGTGCACG TTTCTCCCAC TCTGCAACAG TCCATAGTGG GGATCCTGAG AGAGGACTCA 
CTAGACCCCG AACAGCAGCT CAGAGGAGCA TTAGAGCAAA TCCAAGACAC TGTTTCTGGA 
GCCCTCAAGG AAGAGCTGGC ATTTTTCACG AAAGGTAGAG AGACTCCAGA GAATGTCTCG 
GTGGACATCA AAAAAATAGA ACAAGTTGCA GACAATGGAA CCATGACTAT TGTGGCGGAG 
CTCAATGTGT CCCAGACGTT GGAGGAGTCT GGACTGCTGG AAGAAGCAGA AGATGATCCA 
TCTGAAGAGC AGATCATGGC GGCGCTGAGC TCATCTCATC CAATGCTCCA GCAGGCCATA 
GGTGGAGCTG CCGGTACAGG ATACACCATG AAAATTTCCA AAGAGGAGTT TGAAACGGAC 
GAAGTGCCAT GGATGACCAC CGATGAAGAG TTCCAACAGT GGAGCTCAAC CGATGGGTTT 
GGCAAGACAG AAAAACACAT CAAGCTCGGC CCTACTGAGA GATCTTTTAC TTTTCAGATG 
GATGTGAACA ACGGCTCTAC TGCATCAGTG AGTGAAGGAG CCATCGAAGT GCAAAGCAGC 
TCTTCTGAAT TCTCACAGAC TCAGATGATT GAGCCACACT TAAAGGTCTG TCATGAGAAA 
AGAGTCGCAA CTGTTTATCT TGAAAGCCCC AAAGATGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Extracellular component hyaluronic acid and its receptor Hmmr are required for epicardial EMT during heart regeneration.
Cardiovascular research107(4)487-98(2015 Sep)
Missinato MA,Tobita K,Romano N,Carroll JA,Tsang M

Consequences of lineage-specific gene loss on functional evolution of surviving paralogs: ALDH1A and retinoic acid signaling in vertebrate genomes.
PLoS genetics5(5)e1000496(2009 May)
Ca?estro C,Catchen JM,Rodr?guez-Mar? A,Yokoi H,Postlethwait JH

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synm cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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