Nipa1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nipa1
Entrez Gene ID	233280
Full Name	non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human)
Synonyms	1110027G09Rik,A830014A18Rik,FSP3,Spg6
General protein information	Preferred Names non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human) Names magnesium transporter NIPA1 non-imprinted in Prader-Willi/Angelman syndrome 1 non-imprinted in Prader-Willi/Angelman syndrome region protein 1 homolog spastic paraplegia 6 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_153578.3	NP_705806.1	magnesium transporter NIPA1
NM_153578.2	NP_705806.1	magnesium transporter NIPA1

Pathways from BioSystems

Homologies

	LOC710236	XP_001106326.1
Danio rerio (zebrafish)	nipa1	NP_001006062.1
Homo sapiens (human)	NIPA1	NP_653200.2
Mus musculus (house mouse)	Nipa1	NP_705806.1
Rattus norvegicus (Norway rat)	Nipa1	NP_001100989.1
Gallus gallus (chicken)	NIPA1	XP_003640574.2
Xenopus tropicalis (tropical clawed frog)	nipa1	XP_002937031.1
Canis lupus familiaris (dog)	NIPA1	XP_545798.2
Bos taurus (cattle)	NIPA1	XP_002685238.3

Gene Ontology
Bibliography

Related articles in PubMed

Histopathology reveals correlative and unique phenotypes in a high-throughput mouse phenotyping screen.
Adissu HA, Estabel J, Sunter D, Tuck E, Hooks Y, Carragher DM, Clarke K, Karp NA, , Newbigging S, Jones N, Morikawa L, White JK, McKerlie C
Disease models & mechanisms7(5)515-24(2014 May)

The effect of HSP-causing mutations in SPG3A and NIPA1 on the assembly, trafficking, and interaction between atlastin-1 and NIPA1.
Botzolakis EJ, Zhao J, Gurba KN, Macdonald RL, Hedera P
Molecular and cellular neurosciences46(1)122-35(2011 Jan)

A co-segregating microduplication of chromosome 15q11.2 pinpoints two risk genes for autism spectrum disorder.
van der Zwaag B, Staal WG, Hochstenbach R, Poot M, Spierenburg HA, de Jonge MV, Verbeek NE, van 't Slot R, van Es MA, Staal FJ, Freitag CM, Buizer-Voskamp JE, Nelen MR, van den Berg LH, van Amstel HK, van Engeland H, Burbach JP
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics153B(4)960-6(2010 Jun)

Hereditary spastic paraplegia-associated mutations in the NIPA1 gene and its Caenorhabditis elegans homolog trigger neural degeneration in vitro and in vivo through a gain-of-function mechanism.
Zhao J, Matthies DS, Botzolakis EJ, Macdonald RL, Blakely RD, Hedera P
The Journal of neuroscience : the official journal of the Society for Neuroscience28(51)13938-51(2008 Dec)

NIPA1(SPG6), the basis for autosomal dominant form of hereditary spastic paraplegia, encodes a functional Mg2+ transporter.
Goytain A, Hines RM, El-Husseini A, Quamme GA
The Journal of biological chemistry282(11)8060-8(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

indentification on mouse chromosome 7C
Title: Identification of four highly conserved genes between breakpoint hotspots BP1 and BP2 of the Prader-Willi/Angelman syndromes deletion region that have undergone evolutionary transposition mediated by flanking duplicons.

The following Nipa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nipa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02913	NM_153578.3 Latest version!	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human) (Nipa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OMu02913	NM_153578.2	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human) (Nipa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nipa1 ( NM_153578.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705806.1 Mus musculus non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human) (Nipa1), mRNA.

Price & Availability↑

CloneID	OMu02913
Clone ID Related Accession (Same CDS sequence)	NM_153578.3 , NM_153578.2
Accession Version	NM_153578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	magnesium transporter NIPA1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102121.7. On Jun 9, 2018 this sequence version replaced NM_153578.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC055828.1, AK046619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACTG CGGCGGCGGC TGCGGCGGCC GGGGAGGGGG CGCGCGGCCC GAGCCCCGCC 
GCCGTGTCGC TCGGCCTGGG CGTGGCTGTG GTGTCCAGCC TAGTGAACGG GTCCACGTTC 
GTGCTGCAGA AGAAGGGCAT TGTGCGCGCT AAAAGGCGAG GTACTTCCTA CCTAACAGAC 
ATTGTGTGGT GGGCTGGCAC AATTGCAATG GCTGTCGGCC AGATTGGAAA CTTTCTAGCT 
TACACTGCGG TCCCTACAGT CCTGGTGACT CCCTTAGGTG CCCTTGGAGT ACCGTTTGGG 
TCCATTTTAG CTTCTTACCT TCTGAAGGAA AAGCTCAACA TCTTGGGCAA GTTGGGCTGT 
CTGCTAAGCT GCGCAGGTTC TGTCGTGCTG ATCATCCATT CCCCGAAGTC TGAGAGTGTG 
ACCACGCAGG CCGAGTTGGA GGAGAAGCTG ACCAATCCAG TGTTTGTGGG CTACCTGTGC 
ATCGTGCTGC TCATGCTGCT GCTGTTGATC TTCTGGATCG CACCAGCCCA CGGGCCCACC 
AACATCATGG TCTACATCAG CATCTGCTCC TTGCTGGGGA GTTTCACTGT GCCTTCCACC 
AAGGGCATTG GGCTGGCAGC CCAAGACATC TTGCACAACA ATCCATCCAG TCAGAGAGCC 
CTCTGCCTGT GCCTGGTGCT CCTGGCTGTC CTGGGCTGCA GCATCATTGT CCAATTCAGG 
TACATCAACA AGGCCCTGGA GTGCTTCGAC TCCTCTGTAT TTGGGGCCAT CTACTACGTT 
GTGTTTACCA CGCTGGTCCT ACTGGCTTCA GCCATCCTCT TCCGGGAGTG GAGCAATGTG 
GGCCTGGTGG ACTTCTTGGG CATGGCCTGT GGATTTACAA CTGTCTCCGT GGGAATTGTC 
CTCATACAAG TGTTCAAAGA ATTCAACTTC AACCTTGGAG AGATGAACAA ATCCAATATG 
AAAACAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705806.1
CDS	33..1004
Translation	MGTAAAAAAAGEGARGPSPAAVSLGLGVAVVSSLVNGSTFVLQKKGIVRAKRRGTSYLTDIVWWAGTIAMAVGQIGNFLAYTAVPTVLVTPLGALGVPFGSILASYLLKEKLNILGKLGCLLSCAGSVVLIIHSPKSESVTTQAELEEKLTNPVFVGYLCIVLLMLLLLIFWIAPAHGPTNIMVYISICSLLGSFTVPSTKGIGLAAQDILHNNPSSQRALCLCLVLLAVLGCSIIVQFRYINKALECFDSSVFGAIYYVVFTTLVLLASAILFREWSNVGLVDFLGMACGFTTVSVGIVLIQVFKEFNFNLGEMNKSNMKTD

Target ORF information:

RefSeq Version	NM_153578.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human) (Nipa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153578.3

ORF Insert Sequence:

ATGGGGACTG CGGCGGCGGC TGCGGCGGCC GGGGAGGGGG CGCGCGGCCC GAGCCCCGCC 
GCCGTGTCGC TCGGCCTGGG CGTGGCTGTG GTGTCCAGCC TAGTGAACGG GTCCACGTTC 
GTGCTGCAGA AGAAGGGCAT TGTGCGCGCT AAAAGGCGAG GTACTTCCTA CCTAACAGAC 
ATTGTGTGGT GGGCTGGCAC AATTGCAATG GCTGTCGGCC AGATTGGAAA CTTTCTAGCT 
TACACTGCGG TCCCTACAGT CCTGGTGACT CCCTTAGGTG CCCTTGGAGT ACCGTTTGGG 
TCCATTTTAG CTTCTTACCT TCTGAAGGAA AAGCTCAACA TCTTGGGCAA GTTGGGCTGT 
CTGCTAAGCT GCGCAGGTTC TGTCGTGCTG ATCATCCATT CCCCGAAGTC TGAGAGTGTG 
ACCACGCAGG CCGAGTTGGA GGAGAAGCTG ACCAATCCAG TGTTTGTGGG CTACCTGTGC 
ATCGTGCTGC TCATGCTGCT GCTGTTGATC TTCTGGATCG CACCAGCCCA CGGGCCCACC 
AACATCATGG TCTACATCAG CATCTGCTCC TTGCTGGGGA GTTTCACTGT GCCTTCCACC 
AAGGGCATTG GGCTGGCAGC CCAAGACATC TTGCACAACA ATCCATCCAG TCAGAGAGCC 
CTCTGCCTGT GCCTGGTGCT CCTGGCTGTC CTGGGCTGCA GCATCATTGT CCAATTCAGG 
TACATCAACA AGGCCCTGGA GTGCTTCGAC TCCTCTGTAT TTGGGGCCAT CTACTACGTT 
GTGTTTACCA CGCTGGTCCT ACTGGCTTCA GCCATCCTCT TCCGGGAGTG GAGCAATGTG 
GGCCTGGTGG ACTTCTTGGG CATGGCCTGT GGATTTACAA CTGTCTCCGT GGGAATTGTC 
CTCATACAAG TGTTCAAAGA ATTCAACTTC AACCTTGGAG AGATGAACAA ATCCAATATG 
AAAACAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipa1 ( NM_153578.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705806.1 Mus musculus non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human) (Nipa1), mRNA.

Price & Availability↑

CloneID	OMu02913
Clone ID Related Accession (Same CDS sequence)	NM_153578.3 , NM_153578.2
Accession Version	NM_153578.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-27
Organism	Mus musculus(house mouse)
Product	magnesium transporter NIPA1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC102121.7. On Nov 17, 2006 this sequence version replaced NM_153578.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC055828.1, AY098645.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGGGACTG CGGCGGCGGC TGCGGCGGCC GGGGAGGGGG CGCGCGGCCC GAGCCCCGCC 
GCCGTGTCGC TCGGCCTGGG CGTGGCTGTG GTGTCCAGCC TAGTGAACGG GTCCACGTTC 
GTGCTGCAGA AGAAGGGCAT TGTGCGCGCT AAAAGGCGAG GTACTTCCTA CCTAACAGAC 
ATTGTGTGGT GGGCTGGCAC AATTGCAATG GCTGTCGGCC AGATTGGAAA CTTTCTAGCT 
TACACTGCGG TCCCTACAGT CCTGGTGACT CCCTTAGGTG CCCTTGGAGT ACCGTTTGGG 
TCCATTTTAG CTTCTTACCT TCTGAAGGAA AAGCTCAACA TCTTGGGCAA GTTGGGCTGT 
CTGCTAAGCT GCGCAGGTTC TGTCGTGCTG ATCATCCATT CCCCGAAGTC TGAGAGTGTG 
ACCACGCAGG CCGAGTTGGA GGAGAAGCTG ACCAATCCAG TGTTTGTGGG CTACCTGTGC 
ATCGTGCTGC TCATGCTGCT GCTGTTGATC TTCTGGATCG CACCAGCCCA CGGGCCCACC 
AACATCATGG TCTACATCAG CATCTGCTCC TTGCTGGGGA GTTTCACTGT GCCTTCCACC 
AAGGGCATTG GGCTGGCAGC CCAAGACATC TTGCACAACA ATCCATCCAG TCAGAGAGCC 
CTCTGCCTGT GCCTGGTGCT CCTGGCTGTC CTGGGCTGCA GCATCATTGT CCAATTCAGG 
TACATCAACA AGGCCCTGGA GTGCTTCGAC TCCTCTGTAT TTGGGGCCAT CTACTACGTT 
GTGTTTACCA CGCTGGTCCT ACTGGCTTCA GCCATCCTCT TCCGGGAGTG GAGCAATGTG 
GGCCTGGTGG ACTTCTTGGG CATGGCCTGT GGATTTACAA CTGTCTCCGT GGGAATTGTC 
CTCATACAAG TGTTCAAAGA ATTCAACTTC AACCTTGGAG AGATGAACAA ATCCAATATG 
AAAACAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705806.1
CDS	6..977
Translation	MGTAAAAAAAGEGARGPSPAAVSLGLGVAVVSSLVNGSTFVLQKKGIVRAKRRGTSYLTDIVWWAGTIAMAVGQIGNFLAYTAVPTVLVTPLGALGVPFGSILASYLLKEKLNILGKLGCLLSCAGSVVLIIHSPKSESVTTQAELEEKLTNPVFVGYLCIVLLMLLLLIFWIAPAHGPTNIMVYISICSLLGSFTVPSTKGIGLAAQDILHNNPSSQRALCLCLVLLAVLGCSIIVQFRYINKALECFDSSVFGAIYYVVFTTLVLLASAILFREWSNVGLVDFLGMACGFTTVSVGIVLIQVFKEFNFNLGEMNKSNMKTD

Target ORF information:

RefSeq Version	NM_153578.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human) (Nipa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153578.2

ORF Insert Sequence:

ATGGGGACTG CGGCGGCGGC TGCGGCGGCC GGGGAGGGGG CGCGCGGCCC GAGCCCCGCC 
GCCGTGTCGC TCGGCCTGGG CGTGGCTGTG GTGTCCAGCC TAGTGAACGG GTCCACGTTC 
GTGCTGCAGA AGAAGGGCAT TGTGCGCGCT AAAAGGCGAG GTACTTCCTA CCTAACAGAC 
ATTGTGTGGT GGGCTGGCAC AATTGCAATG GCTGTCGGCC AGATTGGAAA CTTTCTAGCT 
TACACTGCGG TCCCTACAGT CCTGGTGACT CCCTTAGGTG CCCTTGGAGT ACCGTTTGGG 
TCCATTTTAG CTTCTTACCT TCTGAAGGAA AAGCTCAACA TCTTGGGCAA GTTGGGCTGT 
CTGCTAAGCT GCGCAGGTTC TGTCGTGCTG ATCATCCATT CCCCGAAGTC TGAGAGTGTG 
ACCACGCAGG CCGAGTTGGA GGAGAAGCTG ACCAATCCAG TGTTTGTGGG CTACCTGTGC 
ATCGTGCTGC TCATGCTGCT GCTGTTGATC TTCTGGATCG CACCAGCCCA CGGGCCCACC 
AACATCATGG TCTACATCAG CATCTGCTCC TTGCTGGGGA GTTTCACTGT GCCTTCCACC 
AAGGGCATTG GGCTGGCAGC CCAAGACATC TTGCACAACA ATCCATCCAG TCAGAGAGCC 
CTCTGCCTGT GCCTGGTGCT CCTGGCTGTC CTGGGCTGCA GCATCATTGT CCAATTCAGG 
TACATCAACA AGGCCCTGGA GTGCTTCGAC TCCTCTGTAT TTGGGGCCAT CTACTACGTT 
GTGTTTACCA CGCTGGTCCT ACTGGCTTCA GCCATCCTCT TCCGGGAGTG GAGCAATGTG 
GGCCTGGTGG ACTTCTTGGG CATGGCCTGT GGATTTACAA CTGTCTCCGT GGGAATTGTC 
CTCATACAAG TGTTCAAAGA ATTCAACTTC AACCTTGGAG AGATGAACAA ATCCAATATG 
AAAACAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histopathology reveals correlative and unique phenotypes in a high-throughput mouse phenotyping screen.
Disease models & mechanisms7(5)515-24(2014 May)
Adissu HA,Estabel J,Sunter D,Tuck E,Hooks Y,Carragher DM,Clarke K,Karp NA,,Newbigging S,Jones N,Morikawa L,White JK,McKerlie C

The effect of HSP-causing mutations in SPG3A and NIPA1 on the assembly, trafficking, and interaction between atlastin-1 and NIPA1.
Molecular and cellular neurosciences46(1)122-35(2011 Jan)
Botzolakis EJ,Zhao J,Gurba KN,Macdonald RL,Hedera P

A co-segregating microduplication of chromosome 15q11.2 pinpoints two risk genes for autism spectrum disorder.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics153B(4)960-6(2010 Jun)
van der Zwaag B,Staal WG,Hochstenbach R,Poot M,Spierenburg HA,de Jonge MV,Verbeek NE,van 't Slot R,van Es MA,Staal FJ,Freitag CM,Buizer-Voskamp JE,Nelen MR,van den Berg LH,van Amstel HK,van Engeland H,Burbach JP

Hereditary spastic paraplegia-associated mutations in the NIPA1 gene and its Caenorhabditis elegans homolog trigger neural degeneration in vitro and in vivo through a gain-of-function mechanism.
The Journal of neuroscience : the official journal of the Society for Neuroscience28(51)13938-51(2008 Dec)
Zhao J,Matthies DS,Botzolakis EJ,Macdonald RL,Blakely RD,Hedera P

NIPA1(SPG6), the basis for autosomal dominant form of hereditary spastic paraplegia, encodes a functional Mg2+ transporter.
The Journal of biological chemistry282(11)8060-8(2007 Mar)
Goytain A,Hines RM,El-Husseini A,Quamme GA