Nrd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nrd1
Entrez Gene ID	230598
Full Name	nardilysin, N-arginine dibasic convertase, NRD convertase 1
Synonyms	2600011I06Rik,AI875733,NRD-C,Nrdc
General protein information	Preferred Names nardilysin, N-arginine dibasic convertase, NRD convertase 1 Names nardilysin N-arginine dibasic convertase NRD convertase nardilysin convertase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001347169.1	NP_001334098.1	nardilysin
XM_006502991.3	XP_006503054.1	nardilysin isoform X2
NM_001347169.2	NP_001334098.1	nardilysin isoform 1
NM_001346554.1	NP_001333483.1	nardilysin isoform 3
NM_146150.2	NP_666262.2	nardilysin precursor
NM_146150.3	NP_666262.2	nardilysin isoform 2 precursor
XM_017320144.1	XP_017175633.1	nardilysin isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NRD1	XP_003949467.1
Rattus norvegicus (Norway rat)	Nrd1	NP_037125.1
Oryza sativa (rice)	Os03g0336300	NP_001050040.1
Homo sapiens (human)	NRD1	NP_002516.2
Arabidopsis thaliana (thale cress)	AT1G06900	NP_172173.2
Xenopus tropicalis (tropical clawed frog)	nrd1	XP_002931499.2
Mus musculus (house mouse)	Nrd1	NP_666262.2
Gallus gallus (chicken)	NRD1	NP_001026455.1
Canis lupus familiaris (dog)	NRD1	XP_003639566.1
Bos taurus (cattle)	NRD1	NP_001193920.1
Danio rerio (zebrafish)	si:dkey-193b15.9	XP_694205.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide profiling of nardilysin target genes reveals its role in epigenetic regulation and cell cycle progression.
Morita Y, Ohno M, Nishi K, Hiraoka Y, Saijo S, Matsuda S, Kita T, Kimura T, Nishi E
Scientific reports7(1)14801(2017 Nov)

Nardilysin regulates inflammation, metaplasia, and tumors in murine stomach.
Kimura Y, Ikuta K, Kimura T, Chiba T, Oshima H, Oshima M, Nishi E, Seno H
Scientific reports743052(2017 Feb)

Nardilysin Is Required for Maintaining Pancreatic ?-Cell Function.
Nishi K, Sato Y, Ohno M, Hiraoka Y, Saijo S, Sakamoto J, Chen PM, Morita Y, Matsuda S, Iwasaki K, Sugizaki K, Harada N, Mukumoto Y, Kiyonari H, Furuyama K, Kawaguchi Y, Uemoto S, Kita T, Inagaki N, Kimura T, Nishi E
Diabetes65(10)3015-27(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nardilysin Is Required for Maintaining Pancreatic beta-Cell Function
Title: Nardilysin Is Required for Maintaining Pancreatic ?-Cell Function.

deletion of nardilysin prevents the development of diet-induced steatohepatitis and liver fibrogenesis
Title: Deletion of nardilysin prevents the development of steatohepatitis and liver fibrotic changes.

It controls Amyloid beta plaque formation through the regulation of a-secretase.
Title: Nardilysin prevents amyloid plaque formation by enhancing ?-secretase activity in an Alzheimer's disease mouse model.

critical regulator of body temperature homoeostasis
Title: Critical roles of nardilysin in the maintenance of body temperature homoeostasis.

Identification and characterization of nardilysin as a novel dimethyl H3K4-binding protein involved in transcriptional regulation.
Title: Identification and characterization of nardilysin as a novel dimethyl H3K4-binding protein involved in transcriptional regulation.

The following Nrd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nrd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu80951	XM_017320144.1 Latest version!	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu18832	NM_146150.3 Latest version!	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$678.30 $969.00
OMu33518	NM_001347169.2 Latest version!	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu83486	NM_001346554.1 Latest version!	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu33519	XM_006502991.3 Latest version!	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OMu33518	NM_001347169.1	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu18832	NM_146150.2	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$678.30 $969.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nrd1 ( XM_017320144.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175633.1 Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu80951
Clone ID Related Accession (Same CDS sequence)	XM_017320144.1
Accession Version	XM_017320144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nardilysin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTCA TGGGTAGTTT GAAATACCCT GATGAGAATG GATTTGATGC CTTCCTCAAG 
AAACATGGAG GTAGTGATAA TGCCTCAACT GATTGTGAAC GCACAGTCTT TCAGTTTGAT 
GTCCAGAGAA AGTATTTCAA GGAAGCCCTG GATAGATGGG CCCAGTTCTT TATCCATCCA 
CTGATGATCA GAGATGCAAT TGACCGAGAG GTGGAAGCTG TGGACAGTGA GTATCAGCTT 
GCAAGGCCTT CTGATGCAAA CAGAAAAGAA ATGTTGTTTG GAAGCCTCGC TAGACCGGGA 
CATCCTATGG GGAAGTTTTT TTGGGGAAAT GCTGAGACAC TCAAGCATGA ACCAAAGAAG 
AATAACATTG ATACACATGC CAGACTGAGA GAATTCTGGA TGCGCTACTA CTCTGCTCAT 
TACATGACCT TAGTTGTTCA GTCCAAAGAA ACCCTGGACA CTTTGGAAAA GTGGGTGACG 
GAGATCTTCT CTCAGATACC AAACAATGGG CTACCTAAAC CAAACTTTAG CCATTTAACG 
GATCCTTTTG ACACACCAGC ATTTAACAAA CTTTATAGAG TTGTTCCAAT CAGAAAAATC 
CATGCTCTGA CCATCACATG GGCACTTCCT CCTCAGCAGC AGCACTACAG GGTGAAGCCA 
CTTCATTATA TTTCCTGGCT TGTTGGACAT GAAGGCAAAG GCAGCATTCT TTCTTATCTT 
AGAAAAAAGT GCTGGGCTCT TGCATTGTTT GGTGGAAATG GTGAGACAGG TTTTGAGCAA 
AATTCTACTT ACTCAGTGTT CAGCATTTCT ATTACGCTGA CTGATGAGGG CTATGAGCAT 
TTTTATGAGG TTGCCCACAC TGTCTTTCAG TATTTAAAAA TGCTGCAGAA GCTAGGTCCA 
GAGAAAAGAG TTTTTGAAGA GATTCAGAAA ATTGAGGATA ATGAATTTCA TTATCAAGAA 
CAGACAGATC CAGTCGAGTA TGTGGAGAAC ATGTGTGAGA ACATGCAGCT GTACCCACGT 
CAGGACTTTC TCACCGGAGA TCAGCTTCTG TTTGAGTACA AGCCAGAAGT CATTGCTGAG 
GCCCTTAATC AGCTAGTTCC TCAGAAGGCA AATCTCGTTC TTCTCTCTGG TGCTAATGAG 
GGAAGATGTG ACCTCAAGGA GAAGTGGTTT GGGACTCAGT ATAGCATAGA AGATATTGAA 
AACTCTTGGA CTGAACTATG GAAGAGTAAT TTTGACTTAA ATCCAGACCT TCATCTTCCA 
GCTGAAAATA AGTACATAGC CACGGACTTT ACGTTGAAGG CTTTTGATTG CCCTGAAACT 
GAATACCCTG CTAAAATTGT CAATACCGCA CAAGGCTGCT TGTGGTATAA GAAAGACAAC 
AAATTCAAAA TCCCCAAAGC ATATATACGA TTTCATCTGA TCTCACCTTT GATACAGAAA 
TCTGCAGCAA ATGTTGTCCT CTTTGACATC TTCGTGAATA TTCTTACGCA CAACCTTGCC 
GAACCCGCCT ATGAAGCTGA TGTGGCACAG CTGGAGTATA AACTGGTAGC TGGAGAACAT 
GGGTTAATTA TTCGAGTGAA AGGATTCAAC CACAAACTAC CTCTGCTCTT TCAGCTCATT 
ATTGACTACC TGACTGAGTT CAGTTCCACA CCCGCTGTCT TTACCATGAT AACTGAGCAA 
CTGAAGAAGA CCTACTTCAA CATCCTCATC AAGCCCGAGA CTCTGGCCAA AGATGTGCGG 
CTTTTAATTC TGGAATATTC CCGCTGGTCT ATGATTGACA AGTACCAAGC TTTGATGGAT 
GGCCTTTCCC TTGATTCTCT GCTAAACTTT GTCAAGGATT TTAAATCCCA ACTTTTTGTT 
GAGGGCCTGG TCCAAGGGAA TGTCACCAGC ACGGAATCGA TGGATTTCCT AAAATATGTT 
GTTGACAAGC TGAACTTCGC GCCCCTGGAG CGGGAGATGC CTGTGCAGTT CCAGGTGGTG 
GAGCTGCCGA GCGGCCACCA CCTGTGCAAA GTCAGAGCTC TGAATAAAGG GGATGCCAAC 
TCTGAAGTCA CTGTGTACTA CCAGTCAGGT ACGAGGAGTC TGAGAGAATA TACTCTTATG 
GAGCTGCTTG TGATGCACAT GGAAGAGCCC TGCTTTGACT TCCTTCGCAC CAAGCAGACC 
CTTGGGTACC ATGTCTACCC AACCTGTAGG AACACATCTG GAATTCTAGG ATTTTCTGTA 
ACTGTTGGGA CTCAGGCAAC CAAATACAAC TCCGAAACTG TTGACAAGAA GATAGAGGAA 
TTTCTTTCCA GCTTTGAGGA GAAGATTGAG AACCTCACAG AAGATGCATT CAATACTCAG 
GTCACAGCAC TGATCAAGTT GAAAGAGTGT GAGGACACCC ACCTTGGGGA GGAGGTGGAC 
AGGAACTGGA ATGAAGTGGT GACACAGCAG TATCTCTTTG ACCGCCTTGC CCATGAGATT 
GAAGCCCTGA AGTCTTTCTC AAAGTCAGAC CTGGTCAGCT GGTTCAAGGC TCACAGAGGA 
CCTGGAAGTA AAATGCTCAG CGTTCATGTT GTTGGATATG GGAAGTATGA ACTGGAAGAG 
GATGGTGCCC CTTTTGGTGA GGATTCAAAT TCTCGTGAAG GGATGCAGCT AACCTACCTG 
CCACCCTCTC CTGTCCTGGC AGAATCCACC ACCCCCATTA CTGACATCAG GGCCTTCACA 
GCAACACTCA GCCTCTTCCC CTACCATAAA ATAGTCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175633.1
CDS	1001..3742
Translation	MVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKYFKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLKHEPKKNNIDTHARLREFWMRYYSAHYMTLVVQSKETLDTLEKWVTEIFSQIPNNGLPKPNFSHLTDPFDTPAFNKLYRVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGSILSYLRKKCWALALFGGNGETGFEQNSTYSVFSISITLTDEGYEHFYEVAHTVFQYLKMLQKLGPEKRVFEEIQKIEDNEFHYQEQTDPVEYVENMCENMQLYPRQDFLTGDQLLFEYKPEVIAEALNQLVPQKANLVLLSGANEGRCDLKEKWFGTQYSIEDIENSWTELWKSNFDLNPDLHLPAENKYIATDFTLKAFDCPETEYPAKIVNTAQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNHKLPLLFQLIIDYLTEFSSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYSRWSMIDKYQALMDGLSLDSLLNFVKDFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFAPLEREMPVQFQVVELPSGHHLCKVRALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGYHVYPTCRNTSGILGFSVTVGTQATKYNSETVDKKIEEFLSSFEEKIENLTEDAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFDRLAHEIEALKSFSKSDLVSWFKAHRGPGSKMLSVHVVGYGKYELEEDGAPFGEDSNSREGMQLTYLPPSPVLAESTTPITDIRAFTATLSLFPYHKIVK

Target ORF information:

RefSeq Version	XM_017320144.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320144.1

ORF Insert Sequence:

ATGGTATTCA TGGGTAGTTT GAAATACCCT GATGAGAATG GATTTGATGC CTTCCTCAAG 
AAACATGGAG GTAGTGATAA TGCCTCAACT GATTGTGAAC GCACAGTCTT TCAGTTTGAT 
GTCCAGAGAA AGTATTTCAA GGAAGCCCTG GATAGATGGG CCCAGTTCTT TATCCATCCA 
CTGATGATCA GAGATGCAAT TGACCGAGAG GTGGAAGCTG TGGACAGTGA GTATCAGCTT 
GCAAGGCCTT CTGATGCAAA CAGAAAAGAA ATGTTGTTTG GAAGCCTCGC TAGACCGGGA 
CATCCTATGG GGAAGTTTTT TTGGGGAAAT GCTGAGACAC TCAAGCATGA ACCAAAGAAG 
AATAACATTG ATACACATGC CAGACTGAGA GAATTCTGGA TGCGCTACTA CTCTGCTCAT 
TACATGACCT TAGTTGTTCA GTCCAAAGAA ACCCTGGACA CTTTGGAAAA GTGGGTGACG 
GAGATCTTCT CTCAGATACC AAACAATGGG CTACCTAAAC CAAACTTTAG CCATTTAACG 
GATCCTTTTG ACACACCAGC ATTTAACAAA CTTTATAGAG TTGTTCCAAT CAGAAAAATC 
CATGCTCTGA CCATCACATG GGCACTTCCT CCTCAGCAGC AGCACTACAG GGTGAAGCCA 
CTTCATTATA TTTCCTGGCT TGTTGGACAT GAAGGCAAAG GCAGCATTCT TTCTTATCTT 
AGAAAAAAGT GCTGGGCTCT TGCATTGTTT GGTGGAAATG GTGAGACAGG TTTTGAGCAA 
AATTCTACTT ACTCAGTGTT CAGCATTTCT ATTACGCTGA CTGATGAGGG CTATGAGCAT 
TTTTATGAGG TTGCCCACAC TGTCTTTCAG TATTTAAAAA TGCTGCAGAA GCTAGGTCCA 
GAGAAAAGAG TTTTTGAAGA GATTCAGAAA ATTGAGGATA ATGAATTTCA TTATCAAGAA 
CAGACAGATC CAGTCGAGTA TGTGGAGAAC ATGTGTGAGA ACATGCAGCT GTACCCACGT 
CAGGACTTTC TCACCGGAGA TCAGCTTCTG TTTGAGTACA AGCCAGAAGT CATTGCTGAG 
GCCCTTAATC AGCTAGTTCC TCAGAAGGCA AATCTCGTTC TTCTCTCTGG TGCTAATGAG 
GGAAGATGTG ACCTCAAGGA GAAGTGGTTT GGGACTCAGT ATAGCATAGA AGATATTGAA 
AACTCTTGGA CTGAACTATG GAAGAGTAAT TTTGACTTAA ATCCAGACCT TCATCTTCCA 
GCTGAAAATA AGTACATAGC CACGGACTTT ACGTTGAAGG CTTTTGATTG CCCTGAAACT 
GAATACCCTG CTAAAATTGT CAATACCGCA CAAGGCTGCT TGTGGTATAA GAAAGACAAC 
AAATTCAAAA TCCCCAAAGC ATATATACGA TTTCATCTGA TCTCACCTTT GATACAGAAA 
TCTGCAGCAA ATGTTGTCCT CTTTGACATC TTCGTGAATA TTCTTACGCA CAACCTTGCC 
GAACCCGCCT ATGAAGCTGA TGTGGCACAG CTGGAGTATA AACTGGTAGC TGGAGAACAT 
GGGTTAATTA TTCGAGTGAA AGGATTCAAC CACAAACTAC CTCTGCTCTT TCAGCTCATT 
ATTGACTACC TGACTGAGTT CAGTTCCACA CCCGCTGTCT TTACCATGAT AACTGAGCAA 
CTGAAGAAGA CCTACTTCAA CATCCTCATC AAGCCCGAGA CTCTGGCCAA AGATGTGCGG 
CTTTTAATTC TGGAATATTC CCGCTGGTCT ATGATTGACA AGTACCAAGC TTTGATGGAT 
GGCCTTTCCC TTGATTCTCT GCTAAACTTT GTCAAGGATT TTAAATCCCA ACTTTTTGTT 
GAGGGCCTGG TCCAAGGGAA TGTCACCAGC ACGGAATCGA TGGATTTCCT AAAATATGTT 
GTTGACAAGC TGAACTTCGC GCCCCTGGAG CGGGAGATGC CTGTGCAGTT CCAGGTGGTG 
GAGCTGCCGA GCGGCCACCA CCTGTGCAAA GTCAGAGCTC TGAATAAAGG GGATGCCAAC 
TCTGAAGTCA CTGTGTACTA CCAGTCAGGT ACGAGGAGTC TGAGAGAATA TACTCTTATG 
GAGCTGCTTG TGATGCACAT GGAAGAGCCC TGCTTTGACT TCCTTCGCAC CAAGCAGACC 
CTTGGGTACC ATGTCTACCC AACCTGTAGG AACACATCTG GAATTCTAGG ATTTTCTGTA 
ACTGTTGGGA CTCAGGCAAC CAAATACAAC TCCGAAACTG TTGACAAGAA GATAGAGGAA 
TTTCTTTCCA GCTTTGAGGA GAAGATTGAG AACCTCACAG AAGATGCATT CAATACTCAG 
GTCACAGCAC TGATCAAGTT GAAAGAGTGT GAGGACACCC ACCTTGGGGA GGAGGTGGAC 
AGGAACTGGA ATGAAGTGGT GACACAGCAG TATCTCTTTG ACCGCCTTGC CCATGAGATT 
GAAGCCCTGA AGTCTTTCTC AAAGTCAGAC CTGGTCAGCT GGTTCAAGGC TCACAGAGGA 
CCTGGAAGTA AAATGCTCAG CGTTCATGTT GTTGGATATG GGAAGTATGA ACTGGAAGAG 
GATGGTGCCC CTTTTGGTGA GGATTCAAAT TCTCGTGAAG GGATGCAGCT AACCTACCTG 
CCACCCTCTC CTGTCCTGGC AGAATCCACC ACCCCCATTA CTGACATCAG GGCCTTCACA 
GCAACACTCA GCCTCTTCCC CTACCATAAA ATAGTCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrd1 ( NM_146150.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666262.2 Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), mRNA.

Price & Availability↑

CloneID	OMu18832
Clone ID Related Accession (Same CDS sequence)	NM_146150.2 , NM_146150.3
Accession Version	NM_146150.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Mus musculus(House mouse)
Product	nardilysin precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK031548.1. On Jun 10, 2003 this sequence version replaced NM_146150.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031548.1, SRR1660811.150397.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGTCTGCAG CGGCTCTGTG TGTTGGAGTT GGGAGCTTTG CTGATCCAGA TGACCTGCCT 
GGGCTGGCAC ACTTTTTGGA GCACATGGTA TTCATGGGTA GTTTGAAATA CCCTGATGAG 
AATGGATTTG ATGCCTTCCT CAAGAAACAT GGAGGTAGTG ATAATGCCTC AACTGATTGT 
GAACGCACAG TCTTTCAGTT TGATGTCCAG AGAAAGTATT TCAAGGAAGC CCTGGATAGA 
TGGGCCCAGT TCTTTATCCA TCCACTGATG ATCAGAGATG CAATTGACCG AGAGGTGGAA 
GCTGTGGACA GTGAGTATCA GCTTGCAAGG CCTTCTGATG CAAACAGAAA AGAAATGTTG 
TTTGGAAGCC TCGCTAGACC GGGACATCCT ATGGGGAAGT TTTTTTGGGG AAATGCTGAG 
ACACTCAAGC ATGAACCAAA GAAGAATAAC ATTGATACAC ATGCCAGACT GAGAGAATTC 
TGGATGCGCT ACTACTCTGC TCATTACATG ACCTTAGTTG TTCAGTCCAA AGAAACCCTG 
GACACTTTGG AAAAGTGGGT GACGGAGATC TTCTCTCAGA TACCAAACAA TGGGCTACCT 
AAACCAAACT TTAGCCATTT AACGGATCCT TTTGACACAC CAGCATTTAA CAAACTTTAT 
AGAGTTGTTC CAATCAGAAA AATCCATGCT CTGACCATCA CATGGGCACT TCCTCCTCAG 
CAGCAGCACT ACAGGGTGAA GCCACTTCAT TATATTTCCT GGCTTGTTGG ACATGAAGGC 
AAAGGCAGCA TTCTTTCTTA TCTTAGAAAA AAGTGCTGGG CTCTTGCATT GTTTGGTGGA 
AATGGTGAGA CAGGTTTTGA GCAAAATTCT ACTTACTCAG TGTTCAGCAT TTCTATTACG 
CTGACTGATG AGGGCTATGA GCATTTTTAT GAGGTTGCCC ACACTGTCTT TCAGTATTTA 
AAAATGCTGC AGAAGCTAGG TCCAGAGAAA AGAGTTTTTG AAGAGATTCA GAAAATTGAG 
GATAATGAAT TTCATTATCA AGAACAGACA GATCCAGTCG AGTATGTGGA GAACATGTGT 
GAGAACATGC AGCTGTACCC ACGTCAGGAC TTTCTCACCG GAGATCAGCT TCTGTTTGAG 
TACAAGCCAG AAGTCATTGC TGAGGCCCTT AATCAGCTAG TTCCTCAGAA GGCAAATCTC 
GTTCTTCTCT CTGGTGCTAA TGAGGGAAGA TGTGACCTCA AGGAGAAGTG GTTTGGGACT 
CAGTATAGCA TAGAAGATAT TGAAAACTCT TGGACTGAAC TATGGAAGAG TAATTTTGAC 
TTAAATCCAG ACCTTCATCT TCCAGCTGAA AATAAGTACA TAGCCACGGA CTTTACGTTG 
AAGGCTTTTG ATTGCCCTGA AACTGAATAC CCTGCTAAAA TTGTCAATAC CGCACAAGGC 
TGCTTGTGGT ATAAGAAAGA CAACAAATTC AAAATCCCCA AAGCATATAT ACGATTTCAT 
CTGATCTCAC CTTTGATACA GAAATCTGCA GCAAATGTTG TCCTCTTTGA CATCTTCGTG 
AATATTCTTA CGCACAACCT TGCCGAACCC GCCTATGAAG CTGATGTGGC ACAGCTGGAG 
TATAAACTGG TAGCTGGAGA ACATGGGTTA ATTATTCGAG TGAAAGGATT CAACCACAAA 
CTACCTCTGC TCTTTCAGCT CATTATTGAC TACCTGACTG AGTTCAGTTC CACACCCGCT 
GTCTTTACCA TGATAACTGA GCAACTGAAG AAGACCTACT TCAACATCCT CATCAAGCCC 
GAGACTCTGG CCAAAGATGT GCGGCTTTTA ATTCTGGAAT ATTCCCGCTG GTCTATGATT 
GACAAGTACC AAGCTTTGAT GGATGGCCTT TCCCTTGATT CTCTGCTAAA CTTTGTCAAG 
GATTTTAAAT CCCAACTTTT TGTTGAGGGC CTGGTCCAAG GGAATGTCAC CAGCACGGAA 
TCGATGGATT TCCTAAAATA TGTTGTTGAC AAGCTGAACT TCGCGCCCCT GGAGCGGGAG 
ATGCCTGTGC AGTTCCAGGT GGTGGAGCTG CCGAGCGGCC ACCACCTGTG CAAAGTCAGA 
GCTCTGAATA AAGGGGATGC CAACTCTGAA GTCACTGTGT ACTACCAGTC AGGTACGAGG 
AGTCTGAGAG AATATACTCT TATGGAGCTG CTTGTGATGC ACATGGAAGA GCCCTGCTTT 
GACTTCCTTC GCACCAAGCA GACCCTTGGG TACCATGTCT ACCCAACCTG TAGGAACACA 
TCTGGAATTC TAGGATTTTC TGTAACTGTT GGGACTCAGG CAACCAAATA CAACTCCGAA 
ACTGTTGACA AGAAGATAGA GGAATTTCTT TCCAGCTTTG AGGAGAAGAT TGAGAACCTC 
ACAGAAGATG CATTCAATAC TCAGGTCACA GCACTGATCA AGTTGAAAGA GTGTGAGGAC 
ACCCACCTTG GGGAGGAGGT GGACAGGAAC TGGAATGAAG TGGTGACACA GCAGTATCTC 
TTTGACCGCC TTGCCCATGA GATTGAAGCC CTGAAGTCTT TCTCAAAGTC AGACCTGGTC 
AGCTGGTTCA AGGCTCACAG AGGACCTGGA AGTAAAATGC TCAGCGTTCA TGTTGTTGGA 
TATGGGAAGT ATGAACTGGA AGAGGATGGT GCCCCTTTTG GTGAGGATTC AAATTCTCGT 
GAAGGGATGC AGCTAACCTA CCTGCCACCC TCTCCTGTCC TGGCAGAATC CACCACCCCC 
ATTACTGACA TCAGGGCCTT CACAGCAACA CTCAGCCTCT TCCCCTACCA TAAAATAGTC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666262.2
CDS	29..3514
Translation	MLRRVAVAAVCVTGRKLRCEAGRELTALGRIEARGLCEESSKPFPTLTMPGRNKAKSTCSCPDLQPNGQDLGESGRLARLGADESEEEGRSFSNVGDPEIIKSPSDPKQYRYIKLQNGLQALLISDLSNVEGKTGNATDEEEEEEEEEEEEDDDDDDDDDDDDEDSGAEIQDDDEEGFDDEEEFDDDDDDEHDDDDLENEENELEELEERVEARKKTTEKQSAAALCVGVGSFADPDDLPGLAHFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKYFKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLKHEPKKNNIDTHARLREFWMRYYSAHYMTLVVQSKETLDTLEKWVTEIFSQIPNNGLPKPNFSHLTDPFDTPAFNKLYRVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGSILSYLRKKCWALALFGGNGETGFEQNSTYSVFSISITLTDEGYEHFYEVAHTVFQYLKMLQKLGPEKRVFEEIQKIEDNEFHYQEQTDPVEYVENMCENMQLYPRQDFLTGDQLLFEYKPEVIAEALNQLVPQKANLVLLSGANEGRCDLKEKWFGTQYSIEDIENSWTELWKSNFDLNPDLHLPAENKYIATDFTLKAFDCPETEYPAKIVNTAQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNHKLPLLFQLIIDYLTEFSSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYSRWSMIDKYQALMDGLSLDSLLNFVKDFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFAPLEREMPVQFQVVELPSGHHLCKVRALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGYHVYPTCRNTSGILGFSVTVGTQATKYNSETVDKKIEEFLSSFEEKIENLTEDAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFDRLAHEIEALKSFSKSDLVSWFKAHRGPGSKMLSVHVVGYGKYELEEDGAPFGEDSNSREGMQLTYLPPSPVLAESTTPITDIRAFTATLSLFPYHKIVK

Target ORF information:

RefSeq Version	NM_146150.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146150.2

ORF Insert Sequence:

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGTCTGCAG CGGCTCTGTG TGTTGGAGTT GGGAGCTTTG CTGATCCAGA TGACCTGCCT 
GGGCTGGCAC ACTTTTTGGA GCACATGGTA TTCATGGGTA GTTTGAAATA CCCTGATGAG 
AATGGATTTG ATGCCTTCCT CAAGAAACAT GGAGGTAGTG ATAATGCCTC AACTGATTGT 
GAACGCACAG TCTTTCAGTT TGATGTCCAG AGAAAGTATT TCAAGGAAGC CCTGGATAGA 
TGGGCCCAGT TCTTTATCCA TCCACTGATG ATCAGAGATG CAATTGACCG AGAGGTGGAA 
GCTGTGGACA GTGAGTATCA GCTTGCAAGG CCTTCTGATG CAAACAGAAA AGAAATGTTG 
TTTGGAAGCC TCGCTAGACC GGGACATCCT ATGGGGAAGT TTTTTTGGGG AAATGCTGAG 
ACACTCAAGC ATGAACCAAA GAAGAATAAC ATTGATACAC ATGCCAGACT GAGAGAATTC 
TGGATGCGCT ACTACTCTGC TCATTACATG ACCTTAGTTG TTCAGTCCAA AGAAACCCTG 
GACACTTTGG AAAAGTGGGT GACGGAGATC TTCTCTCAGA TACCAAACAA TGGGCTACCT 
AAACCAAACT TTAGCCATTT AACGGATCCT TTTGACACAC CAGCATTTAA CAAACTTTAT 
AGAGTTGTTC CAATCAGAAA AATCCATGCT CTGACCATCA CATGGGCACT TCCTCCTCAG 
CAGCAGCACT ACAGGGTGAA GCCACTTCAT TATATTTCCT GGCTTGTTGG ACATGAAGGC 
AAAGGCAGCA TTCTTTCTTA TCTTAGAAAA AAGTGCTGGG CTCTTGCATT GTTTGGTGGA 
AATGGTGAGA CAGGTTTTGA GCAAAATTCT ACTTACTCAG TGTTCAGCAT TTCTATTACG 
CTGACTGATG AGGGCTATGA GCATTTTTAT GAGGTTGCCC ACACTGTCTT TCAGTATTTA 
AAAATGCTGC AGAAGCTAGG TCCAGAGAAA AGAGTTTTTG AAGAGATTCA GAAAATTGAG 
GATAATGAAT TTCATTATCA AGAACAGACA GATCCAGTCG AGTATGTGGA GAACATGTGT 
GAGAACATGC AGCTGTACCC ACGTCAGGAC TTTCTCACCG GAGATCAGCT TCTGTTTGAG 
TACAAGCCAG AAGTCATTGC TGAGGCCCTT AATCAGCTAG TTCCTCAGAA GGCAAATCTC 
GTTCTTCTCT CTGGTGCTAA TGAGGGAAGA TGTGACCTCA AGGAGAAGTG GTTTGGGACT 
CAGTATAGCA TAGAAGATAT TGAAAACTCT TGGACTGAAC TATGGAAGAG TAATTTTGAC 
TTAAATCCAG ACCTTCATCT TCCAGCTGAA AATAAGTACA TAGCCACGGA CTTTACGTTG 
AAGGCTTTTG ATTGCCCTGA AACTGAATAC CCTGCTAAAA TTGTCAATAC CGCACAAGGC 
TGCTTGTGGT ATAAGAAAGA CAACAAATTC AAAATCCCCA AAGCATATAT ACGATTTCAT 
CTGATCTCAC CTTTGATACA GAAATCTGCA GCAAATGTTG TCCTCTTTGA CATCTTCGTG 
AATATTCTTA CGCACAACCT TGCCGAACCC GCCTATGAAG CTGATGTGGC ACAGCTGGAG 
TATAAACTGG TAGCTGGAGA ACATGGGTTA ATTATTCGAG TGAAAGGATT CAACCACAAA 
CTACCTCTGC TCTTTCAGCT CATTATTGAC TACCTGACTG AGTTCAGTTC CACACCCGCT 
GTCTTTACCA TGATAACTGA GCAACTGAAG AAGACCTACT TCAACATCCT CATCAAGCCC 
GAGACTCTGG CCAAAGATGT GCGGCTTTTA ATTCTGGAAT ATTCCCGCTG GTCTATGATT 
GACAAGTACC AAGCTTTGAT GGATGGCCTT TCCCTTGATT CTCTGCTAAA CTTTGTCAAG 
GATTTTAAAT CCCAACTTTT TGTTGAGGGC CTGGTCCAAG GGAATGTCAC CAGCACGGAA 
TCGATGGATT TCCTAAAATA TGTTGTTGAC AAGCTGAACT TCGCGCCCCT GGAGCGGGAG 
ATGCCTGTGC AGTTCCAGGT GGTGGAGCTG CCGAGCGGCC ACCACCTGTG CAAAGTCAGA 
GCTCTGAATA AAGGGGATGC CAACTCTGAA GTCACTGTGT ACTACCAGTC AGGTACGAGG 
AGTCTGAGAG AATATACTCT TATGGAGCTG CTTGTGATGC ACATGGAAGA GCCCTGCTTT 
GACTTCCTTC GCACCAAGCA GACCCTTGGG TACCATGTCT ACCCAACCTG TAGGAACACA 
TCTGGAATTC TAGGATTTTC TGTAACTGTT GGGACTCAGG CAACCAAATA CAACTCCGAA 
ACTGTTGACA AGAAGATAGA GGAATTTCTT TCCAGCTTTG AGGAGAAGAT TGAGAACCTC 
ACAGAAGATG CATTCAATAC TCAGGTCACA GCACTGATCA AGTTGAAAGA GTGTGAGGAC 
ACCCACCTTG GGGAGGAGGT GGACAGGAAC TGGAATGAAG TGGTGACACA GCAGTATCTC 
TTTGACCGCC TTGCCCATGA GATTGAAGCC CTGAAGTCTT TCTCAAAGTC AGACCTGGTC 
AGCTGGTTCA AGGCTCACAG AGGACCTGGA AGTAAAATGC TCAGCGTTCA TGTTGTTGGA 
TATGGGAAGT ATGAACTGGA AGAGGATGGT GCCCCTTTTG GTGAGGATTC AAATTCTCGT 
GAAGGGATGC AGCTAACCTA CCTGCCACCC TCTCCTGTCC TGGCAGAATC CACCACCCCC 
ATTACTGACA TCAGGGCCTT CACAGCAACA CTCAGCCTCT TCCCCTACCA TAAAATAGTC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrd1 ( NM_146150.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666262.2 Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant 2, mRNA.

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CloneID	OMu18832
Clone ID Related Accession (Same CDS sequence)	NM_146150.2 , NM_146150.3
Accession Version	NM_146150.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-20
Organism	Mus musculus(house mouse)
Product	nardilysin isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY732391.1, CF168810.1 and AK031548.1. On Oct 4, 2017 this sequence version replaced NM_146150.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031548.1, SRR1660815.276224.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGTCTGCAG CGGCTCTGTG TGTTGGAGTT GGGAGCTTTG CTGATCCAGA TGACCTGCCT 
GGGCTGGCAC ACTTTTTGGA GCACATGGTA TTCATGGGTA GTTTGAAATA CCCTGATGAG 
AATGGATTTG ATGCCTTCCT CAAGAAACAT GGAGGTAGTG ATAATGCCTC AACTGATTGT 
GAACGCACAG TCTTTCAGTT TGATGTCCAG AGAAAGTATT TCAAGGAAGC CCTGGATAGA 
TGGGCCCAGT TCTTTATCCA TCCACTGATG ATCAGAGATG CAATTGACCG AGAGGTGGAA 
GCTGTGGACA GTGAGTATCA GCTTGCAAGG CCTTCTGATG CAAACAGAAA AGAAATGTTG 
TTTGGAAGCC TCGCTAGACC GGGACATCCT ATGGGGAAGT TTTTTTGGGG AAATGCTGAG 
ACACTCAAGC ATGAACCAAA GAAGAATAAC ATTGATACAC ATGCCAGACT GAGAGAATTC 
TGGATGCGCT ACTACTCTGC TCATTACATG ACCTTAGTTG TTCAGTCCAA AGAAACCCTG 
GACACTTTGG AAAAGTGGGT GACGGAGATC TTCTCTCAGA TACCAAACAA TGGGCTACCT 
AAACCAAACT TTAGCCATTT AACGGATCCT TTTGACACAC CAGCATTTAA CAAACTTTAT 
AGAGTTGTTC CAATCAGAAA AATCCATGCT CTGACCATCA CATGGGCACT TCCTCCTCAG 
CAGCAGCACT ACAGGGTGAA GCCACTTCAT TATATTTCCT GGCTTGTTGG ACATGAAGGC 
AAAGGCAGCA TTCTTTCTTA TCTTAGAAAA AAGTGCTGGG CTCTTGCATT GTTTGGTGGA 
AATGGTGAGA CAGGTTTTGA GCAAAATTCT ACTTACTCAG TGTTCAGCAT TTCTATTACG 
CTGACTGATG AGGGCTATGA GCATTTTTAT GAGGTTGCCC ACACTGTCTT TCAGTATTTA 
AAAATGCTGC AGAAGCTAGG TCCAGAGAAA AGAGTTTTTG AAGAGATTCA GAAAATTGAG 
GATAATGAAT TTCATTATCA AGAACAGACA GATCCAGTCG AGTATGTGGA GAACATGTGT 
GAGAACATGC AGCTGTACCC ACGTCAGGAC TTTCTCACCG GAGATCAGCT TCTGTTTGAG 
TACAAGCCAG AAGTCATTGC TGAGGCCCTT AATCAGCTAG TTCCTCAGAA GGCAAATCTC 
GTTCTTCTCT CTGGTGCTAA TGAGGGAAGA TGTGACCTCA AGGAGAAGTG GTTTGGGACT 
CAGTATAGCA TAGAAGATAT TGAAAACTCT TGGACTGAAC TATGGAAGAG TAATTTTGAC 
TTAAATCCAG ACCTTCATCT TCCAGCTGAA AATAAGTACA TAGCCACGGA CTTTACGTTG 
AAGGCTTTTG ATTGCCCTGA AACTGAATAC CCTGCTAAAA TTGTCAATAC CGCACAAGGC 
TGCTTGTGGT ATAAGAAAGA CAACAAATTC AAAATCCCCA AAGCATATAT ACGATTTCAT 
CTGATCTCAC CTTTGATACA GAAATCTGCA GCAAATGTTG TCCTCTTTGA CATCTTCGTG 
AATATTCTTA CGCACAACCT TGCCGAACCC GCCTATGAAG CTGATGTGGC ACAGCTGGAG 
TATAAACTGG TAGCTGGAGA ACATGGGTTA ATTATTCGAG TGAAAGGATT CAACCACAAA 
CTACCTCTGC TCTTTCAGCT CATTATTGAC TACCTGACTG AGTTCAGTTC CACACCCGCT 
GTCTTTACCA TGATAACTGA GCAACTGAAG AAGACCTACT TCAACATCCT CATCAAGCCC 
GAGACTCTGG CCAAAGATGT GCGGCTTTTA ATTCTGGAAT ATTCCCGCTG GTCTATGATT 
GACAAGTACC AAGCTTTGAT GGATGGCCTT TCCCTTGATT CTCTGCTAAA CTTTGTCAAG 
GATTTTAAAT CCCAACTTTT TGTTGAGGGC CTGGTCCAAG GGAATGTCAC CAGCACGGAA 
TCGATGGATT TCCTAAAATA TGTTGTTGAC AAGCTGAACT TCGCGCCCCT GGAGCGGGAG 
ATGCCTGTGC AGTTCCAGGT GGTGGAGCTG CCGAGCGGCC ACCACCTGTG CAAAGTCAGA 
GCTCTGAATA AAGGGGATGC CAACTCTGAA GTCACTGTGT ACTACCAGTC AGGTACGAGG 
AGTCTGAGAG AATATACTCT TATGGAGCTG CTTGTGATGC ACATGGAAGA GCCCTGCTTT 
GACTTCCTTC GCACCAAGCA GACCCTTGGG TACCATGTCT ACCCAACCTG TAGGAACACA 
TCTGGAATTC TAGGATTTTC TGTAACTGTT GGGACTCAGG CAACCAAATA CAACTCCGAA 
ACTGTTGACA AGAAGATAGA GGAATTTCTT TCCAGCTTTG AGGAGAAGAT TGAGAACCTC 
ACAGAAGATG CATTCAATAC TCAGGTCACA GCACTGATCA AGTTGAAAGA GTGTGAGGAC 
ACCCACCTTG GGGAGGAGGT GGACAGGAAC TGGAATGAAG TGGTGACACA GCAGTATCTC 
TTTGACCGCC TTGCCCATGA GATTGAAGCC CTGAAGTCTT TCTCAAAGTC AGACCTGGTC 
AGCTGGTTCA AGGCTCACAG AGGACCTGGA AGTAAAATGC TCAGCGTTCA TGTTGTTGGA 
TATGGGAAGT ATGAACTGGA AGAGGATGGT GCCCCTTTTG GTGAGGATTC AAATTCTCGT 
GAAGGGATGC AGCTAACCTA CCTGCCACCC TCTCCTGTCC TGGCAGAATC CACCACCCCC 
ATTACTGACA TCAGGGCCTT CACAGCAACA CTCAGCCTCT TCCCCTACCA TAAAATAGTC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666262.2
CDS	179..3664
Translation	MLRRVAVAAVCVTGRKLRCEAGRELTALGRIEARGLCEESSKPFPTLTMPGRNKAKSTCSCPDLQPNGQDLGESGRLARLGADESEEEGRSFSNVGDPEIIKSPSDPKQYRYIKLQNGLQALLISDLSNVEGKTGNATDEEEEEEEEEEEEDDDDDDDDDDDDEDSGAEIQDDDEEGFDDEEEFDDDDDDEHDDDDLENEENELEELEERVEARKKTTEKQSAAALCVGVGSFADPDDLPGLAHFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKYFKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLKHEPKKNNIDTHARLREFWMRYYSAHYMTLVVQSKETLDTLEKWVTEIFSQIPNNGLPKPNFSHLTDPFDTPAFNKLYRVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGSILSYLRKKCWALALFGGNGETGFEQNSTYSVFSISITLTDEGYEHFYEVAHTVFQYLKMLQKLGPEKRVFEEIQKIEDNEFHYQEQTDPVEYVENMCENMQLYPRQDFLTGDQLLFEYKPEVIAEALNQLVPQKANLVLLSGANEGRCDLKEKWFGTQYSIEDIENSWTELWKSNFDLNPDLHLPAENKYIATDFTLKAFDCPETEYPAKIVNTAQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNHKLPLLFQLIIDYLTEFSSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYSRWSMIDKYQALMDGLSLDSLLNFVKDFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFAPLEREMPVQFQVVELPSGHHLCKVRALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGYHVYPTCRNTSGILGFSVTVGTQATKYNSETVDKKIEEFLSSFEEKIENLTEDAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFDRLAHEIEALKSFSKSDLVSWFKAHRGPGSKMLSVHVVGYGKYELEEDGAPFGEDSNSREGMQLTYLPPSPVLAESTTPITDIRAFTATLSLFPYHKIVK

Target ORF information:

RefSeq Version	NM_146150.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146150.3

ORF Insert Sequence:

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGTCTGCAG CGGCTCTGTG TGTTGGAGTT GGGAGCTTTG CTGATCCAGA TGACCTGCCT 
GGGCTGGCAC ACTTTTTGGA GCACATGGTA TTCATGGGTA GTTTGAAATA CCCTGATGAG 
AATGGATTTG ATGCCTTCCT CAAGAAACAT GGAGGTAGTG ATAATGCCTC AACTGATTGT 
GAACGCACAG TCTTTCAGTT TGATGTCCAG AGAAAGTATT TCAAGGAAGC CCTGGATAGA 
TGGGCCCAGT TCTTTATCCA TCCACTGATG ATCAGAGATG CAATTGACCG AGAGGTGGAA 
GCTGTGGACA GTGAGTATCA GCTTGCAAGG CCTTCTGATG CAAACAGAAA AGAAATGTTG 
TTTGGAAGCC TCGCTAGACC GGGACATCCT ATGGGGAAGT TTTTTTGGGG AAATGCTGAG 
ACACTCAAGC ATGAACCAAA GAAGAATAAC ATTGATACAC ATGCCAGACT GAGAGAATTC 
TGGATGCGCT ACTACTCTGC TCATTACATG ACCTTAGTTG TTCAGTCCAA AGAAACCCTG 
GACACTTTGG AAAAGTGGGT GACGGAGATC TTCTCTCAGA TACCAAACAA TGGGCTACCT 
AAACCAAACT TTAGCCATTT AACGGATCCT TTTGACACAC CAGCATTTAA CAAACTTTAT 
AGAGTTGTTC CAATCAGAAA AATCCATGCT CTGACCATCA CATGGGCACT TCCTCCTCAG 
CAGCAGCACT ACAGGGTGAA GCCACTTCAT TATATTTCCT GGCTTGTTGG ACATGAAGGC 
AAAGGCAGCA TTCTTTCTTA TCTTAGAAAA AAGTGCTGGG CTCTTGCATT GTTTGGTGGA 
AATGGTGAGA CAGGTTTTGA GCAAAATTCT ACTTACTCAG TGTTCAGCAT TTCTATTACG 
CTGACTGATG AGGGCTATGA GCATTTTTAT GAGGTTGCCC ACACTGTCTT TCAGTATTTA 
AAAATGCTGC AGAAGCTAGG TCCAGAGAAA AGAGTTTTTG AAGAGATTCA GAAAATTGAG 
GATAATGAAT TTCATTATCA AGAACAGACA GATCCAGTCG AGTATGTGGA GAACATGTGT 
GAGAACATGC AGCTGTACCC ACGTCAGGAC TTTCTCACCG GAGATCAGCT TCTGTTTGAG 
TACAAGCCAG AAGTCATTGC TGAGGCCCTT AATCAGCTAG TTCCTCAGAA GGCAAATCTC 
GTTCTTCTCT CTGGTGCTAA TGAGGGAAGA TGTGACCTCA AGGAGAAGTG GTTTGGGACT 
CAGTATAGCA TAGAAGATAT TGAAAACTCT TGGACTGAAC TATGGAAGAG TAATTTTGAC 
TTAAATCCAG ACCTTCATCT TCCAGCTGAA AATAAGTACA TAGCCACGGA CTTTACGTTG 
AAGGCTTTTG ATTGCCCTGA AACTGAATAC CCTGCTAAAA TTGTCAATAC CGCACAAGGC 
TGCTTGTGGT ATAAGAAAGA CAACAAATTC AAAATCCCCA AAGCATATAT ACGATTTCAT 
CTGATCTCAC CTTTGATACA GAAATCTGCA GCAAATGTTG TCCTCTTTGA CATCTTCGTG 
AATATTCTTA CGCACAACCT TGCCGAACCC GCCTATGAAG CTGATGTGGC ACAGCTGGAG 
TATAAACTGG TAGCTGGAGA ACATGGGTTA ATTATTCGAG TGAAAGGATT CAACCACAAA 
CTACCTCTGC TCTTTCAGCT CATTATTGAC TACCTGACTG AGTTCAGTTC CACACCCGCT 
GTCTTTACCA TGATAACTGA GCAACTGAAG AAGACCTACT TCAACATCCT CATCAAGCCC 
GAGACTCTGG CCAAAGATGT GCGGCTTTTA ATTCTGGAAT ATTCCCGCTG GTCTATGATT 
GACAAGTACC AAGCTTTGAT GGATGGCCTT TCCCTTGATT CTCTGCTAAA CTTTGTCAAG 
GATTTTAAAT CCCAACTTTT TGTTGAGGGC CTGGTCCAAG GGAATGTCAC CAGCACGGAA 
TCGATGGATT TCCTAAAATA TGTTGTTGAC AAGCTGAACT TCGCGCCCCT GGAGCGGGAG 
ATGCCTGTGC AGTTCCAGGT GGTGGAGCTG CCGAGCGGCC ACCACCTGTG CAAAGTCAGA 
GCTCTGAATA AAGGGGATGC CAACTCTGAA GTCACTGTGT ACTACCAGTC AGGTACGAGG 
AGTCTGAGAG AATATACTCT TATGGAGCTG CTTGTGATGC ACATGGAAGA GCCCTGCTTT 
GACTTCCTTC GCACCAAGCA GACCCTTGGG TACCATGTCT ACCCAACCTG TAGGAACACA 
TCTGGAATTC TAGGATTTTC TGTAACTGTT GGGACTCAGG CAACCAAATA CAACTCCGAA 
ACTGTTGACA AGAAGATAGA GGAATTTCTT TCCAGCTTTG AGGAGAAGAT TGAGAACCTC 
ACAGAAGATG CATTCAATAC TCAGGTCACA GCACTGATCA AGTTGAAAGA GTGTGAGGAC 
ACCCACCTTG GGGAGGAGGT GGACAGGAAC TGGAATGAAG TGGTGACACA GCAGTATCTC 
TTTGACCGCC TTGCCCATGA GATTGAAGCC CTGAAGTCTT TCTCAAAGTC AGACCTGGTC 
AGCTGGTTCA AGGCTCACAG AGGACCTGGA AGTAAAATGC TCAGCGTTCA TGTTGTTGGA 
TATGGGAAGT ATGAACTGGA AGAGGATGGT GCCCCTTTTG GTGAGGATTC AAATTCTCGT 
GAAGGGATGC AGCTAACCTA CCTGCCACCC TCTCCTGTCC TGGCAGAATC CACCACCCCC 
ATTACTGACA TCAGGGCCTT CACAGCAACA CTCAGCCTCT TCCCCTACCA TAAAATAGTC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrd1 ( NM_001347169.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334098.1 Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), mRNA.

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CloneID	OMu33518
Clone ID Related Accession (Same CDS sequence)	NM_001347169.1 , NM_001347169.2
Accession Version	NM_001347169.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-14
Organism	Mus musculus(House mouse)
Product	nardilysin
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627406.15. On Nov 17, 2016 this sequence version replaced XM_006502990.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.238729.1, SRR1660815.262097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGCAATCGC AGAACCTGTT TTTGCTGTGG TCAAAGCTGA CTGATAGACT GTGGTTTAAG 
TCATCTTATT CAAAAATGTC TTCAACCCTG CTGGTCGAGA CAAGAAATCT TTATGGGGTA 
GTTGGAGCTG AAAGCAGGTC TGCACCTGTT GAGCATTTGG CAGGATGGCA AGTGGAGGAG 
CAGCAGGGTG AAACTGACAC AGTGCTGTCT GCAGCGGCTC TGTGTGTTGG AGTTGGGAGC 
TTTGCTGATC CAGATGACCT GCCTGGGCTG GCACACTTTT TGGAGCACAT GGTATTCATG 
GGTAGTTTGA AATACCCTGA TGAGAATGGA TTTGATGCCT TCCTCAAGAA ACATGGAGGT 
AGTGATAATG CCTCAACTGA TTGTGAACGC ACAGTCTTTC AGTTTGATGT CCAGAGAAAG 
TATTTCAAGG AAGCCCTGGA TAGATGGGCC CAGTTCTTTA TCCATCCACT GATGATCAGA 
GATGCAATTG ACCGAGAGGT GGAAGCTGTG GACAGTGAGT ATCAGCTTGC AAGGCCTTCT 
GATGCAAACA GAAAAGAAAT GTTGTTTGGA AGCCTCGCTA GACCGGGACA TCCTATGGGG 
AAGTTTTTTT GGGGAAATGC TGAGACACTC AAGCATGAAC CAAAGAAGAA TAACATTGAT 
ACACATGCCA GACTGAGAGA ATTCTGGATG CGCTACTACT CTGCTCATTA CATGACCTTA 
GTTGTTCAGT CCAAAGAAAC CCTGGACACT TTGGAAAAGT GGGTGACGGA GATCTTCTCT 
CAGATACCAA ACAATGGGCT ACCTAAACCA AACTTTAGCC ATTTAACGGA TCCTTTTGAC 
ACACCAGCAT TTAACAAACT TTATAGAGTT GTTCCAATCA GAAAAATCCA TGCTCTGACC 
ATCACATGGG CACTTCCTCC TCAGCAGCAG CACTACAGGG TGAAGCCACT TCATTATATT 
TCCTGGCTTG TTGGACATGA AGGCAAAGGC AGCATTCTTT CTTATCTTAG AAAAAAGTGC 
TGGGCTCTTG CATTGTTTGG TGGAAATGGT GAGACAGGTT TTGAGCAAAA TTCTACTTAC 
TCAGTGTTCA GCATTTCTAT TACGCTGACT GATGAGGGCT ATGAGCATTT TTATGAGGTT 
GCCCACACTG TCTTTCAGTA TTTAAAAATG CTGCAGAAGC TAGGTCCAGA GAAAAGAGTT 
TTTGAAGAGA TTCAGAAAAT TGAGGATAAT GAATTTCATT ATCAAGAACA GACAGATCCA 
GTCGAGTATG TGGAGAACAT GTGTGAGAAC ATGCAGCTGT ACCCACGTCA GGACTTTCTC 
ACCGGAGATC AGCTTCTGTT TGAGTACAAG CCAGAAGTCA TTGCTGAGGC CCTTAATCAG 
CTAGTTCCTC AGAAGGCAAA TCTCGTTCTT CTCTCTGGTG CTAATGAGGG AAGATGTGAC 
CTCAAGGAGA AGTGGTTTGG GACTCAGTAT AGCATAGAAG ATATTGAAAA CTCTTGGACT 
GAACTATGGA AGAGTAATTT TGACTTAAAT CCAGACCTTC ATCTTCCAGC TGAAAATAAG 
TACATAGCCA CGGACTTTAC GTTGAAGGCT TTTGATTGCC CTGAAACTGA ATACCCTGCT 
AAAATTGTCA ATACCGCACA AGGCTGCTTG TGGTATAAGA AAGACAACAA ATTCAAAATC 
CCCAAAGCAT ATATACGATT TCATCTGATC TCACCTTTGA TACAGAAATC TGCAGCAAAT 
GTTGTCCTCT TTGACATCTT CGTGAATATT CTTACGCACA ACCTTGCCGA ACCCGCCTAT 
GAAGCTGATG TGGCACAGCT GGAGTATAAA CTGGTAGCTG GAGAACATGG GTTAATTATT 
CGAGTGAAAG GATTCAACCA CAAACTACCT CTGCTCTTTC AGCTCATTAT TGACTACCTG 
ACTGAGTTCA GTTCCACACC CGCTGTCTTT ACCATGATAA CTGAGCAACT GAAGAAGACC 
TACTTCAACA TCCTCATCAA GCCCGAGACT CTGGCCAAAG ATGTGCGGCT TTTAATTCTG 
GAATATTCCC GCTGGTCTAT GATTGACAAG TACCAAGCTT TGATGGATGG CCTTTCCCTT 
GATTCTCTGC TAAACTTTGT CAAGGATTTT AAATCCCAAC TTTTTGTTGA GGGCCTGGTC 
CAAGGGAATG TCACCAGCAC GGAATCGATG GATTTCCTAA AATATGTTGT TGACAAGCTG 
AACTTCGCGC CCCTGGAGCG GGAGATGCCT GTGCAGTTCC AGGTGGTGGA GCTGCCGAGC 
GGCCACCACC TGTGCAAAGT CAGAGCTCTG AATAAAGGGG ATGCCAACTC TGAAGTCACT 
GTGTACTACC AGTCAGGTAC GAGGAGTCTG AGAGAATATA CTCTTATGGA GCTGCTTGTG 
ATGCACATGG AAGAGCCCTG CTTTGACTTC CTTCGCACCA AGCAGACCCT TGGGTACCAT 
GTCTACCCAA CCTGTAGGAA CACATCTGGA ATTCTAGGAT TTTCTGTAAC TGTTGGGACT 
CAGGCAACCA AATACAACTC CGAAACTGTT GACAAGAAGA TAGAGGAATT TCTTTCCAGC 
TTTGAGGAGA AGATTGAGAA CCTCACAGAA GATGCATTCA ATACTCAGGT CACAGCACTG 
ATCAAGTTGA AAGAGTGTGA GGACACCCAC CTTGGGGAGG AGGTGGACAG GAACTGGAAT 
GAAGTGGTGA CACAGCAGTA TCTCTTTGAC CGCCTTGCCC ATGAGATTGA AGCCCTGAAG 
TCTTTCTCAA AGTCAGACCT GGTCAGCTGG TTCAAGGCTC ACAGAGGACC TGGAAGTAAA 
ATGCTCAGCG TTCATGTTGT TGGATATGGG AAGTATGAAC TGGAAGAGGA TGGTGCCCCT 
TTTGGTGAGG ATTCAAATTC TCGTGAAGGG ATGCAGCTAA CCTACCTGCC ACCCTCTCCT 
GTCCTGGCAG AATCCACCAC CCCCATTACT GACATCAGGG CCTTCACAGC AACACTCAGC 
CTCTTCCCCT ACCATAAAAT AGTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334098.1
CDS	196..3885
Translation	MLRRVAVAAVCVTGRKLRCEAGRELTALGRIEARGLCEESSKPFPTLTMPGRNKAKSTCSCPDLQPNGQDLGESGRLARLGADESEEEGRSFSNVGDPEIIKSPSDPKQYRYIKLQNGLQALLISDLSNVEGKTGNATDEEEEEEEEEEEEDDDDDDDDDDDDEDSGAEIQDDDEEGFDDEEEFDDDDDDEHDDDDLENEENELEELEERVEARKKTTEKQQSQNLFLLWSKLTDRLWFKSSYSKMSSTLLVETRNLYGVVGAESRSAPVEHLAGWQVEEQQGETDTVLSAAALCVGVGSFADPDDLPGLAHFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKYFKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLKHEPKKNNIDTHARLREFWMRYYSAHYMTLVVQSKETLDTLEKWVTEIFSQIPNNGLPKPNFSHLTDPFDTPAFNKLYRVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGSILSYLRKKCWALALFGGNGETGFEQNSTYSVFSISITLTDEGYEHFYEVAHTVFQYLKMLQKLGPEKRVFEEIQKIEDNEFHYQEQTDPVEYVENMCENMQLYPRQDFLTGDQLLFEYKPEVIAEALNQLVPQKANLVLLSGANEGRCDLKEKWFGTQYSIEDIENSWTELWKSNFDLNPDLHLPAENKYIATDFTLKAFDCPETEYPAKIVNTAQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNHKLPLLFQLIIDYLTEFSSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYSRWSMIDKYQALMDGLSLDSLLNFVKDFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFAPLEREMPVQFQVVELPSGHHLCKVRALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGYHVYPTCRNTSGILGFSVTVGTQATKYNSETVDKKIEEFLSSFEEKIENLTEDAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFDRLAHEIEALKSFSKSDLVSWFKAHRGPGSKMLSVHVVGYGKYELEEDGAPFGEDSNSREGMQLTYLPPSPVLAESTTPITDIRAFTATLSLFPYHKIVK

Target ORF information:

RefSeq Version	NM_001347169.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347169.1

ORF Insert Sequence:

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGCAATCGC AGAACCTGTT TTTGCTGTGG TCAAAGCTGA CTGATAGACT GTGGTTTAAG 
TCATCTTATT CAAAAATGTC TTCAACCCTG CTGGTCGAGA CAAGAAATCT TTATGGGGTA 
GTTGGAGCTG AAAGCAGGTC TGCACCTGTT GAGCATTTGG CAGGATGGCA AGTGGAGGAG 
CAGCAGGGTG AAACTGACAC AGTGCTGTCT GCAGCGGCTC TGTGTGTTGG AGTTGGGAGC 
TTTGCTGATC CAGATGACCT GCCTGGGCTG GCACACTTTT TGGAGCACAT GGTATTCATG 
GGTAGTTTGA AATACCCTGA TGAGAATGGA TTTGATGCCT TCCTCAAGAA ACATGGAGGT 
AGTGATAATG CCTCAACTGA TTGTGAACGC ACAGTCTTTC AGTTTGATGT CCAGAGAAAG 
TATTTCAAGG AAGCCCTGGA TAGATGGGCC CAGTTCTTTA TCCATCCACT GATGATCAGA 
GATGCAATTG ACCGAGAGGT GGAAGCTGTG GACAGTGAGT ATCAGCTTGC AAGGCCTTCT 
GATGCAAACA GAAAAGAAAT GTTGTTTGGA AGCCTCGCTA GACCGGGACA TCCTATGGGG 
AAGTTTTTTT GGGGAAATGC TGAGACACTC AAGCATGAAC CAAAGAAGAA TAACATTGAT 
ACACATGCCA GACTGAGAGA ATTCTGGATG CGCTACTACT CTGCTCATTA CATGACCTTA 
GTTGTTCAGT CCAAAGAAAC CCTGGACACT TTGGAAAAGT GGGTGACGGA GATCTTCTCT 
CAGATACCAA ACAATGGGCT ACCTAAACCA AACTTTAGCC ATTTAACGGA TCCTTTTGAC 
ACACCAGCAT TTAACAAACT TTATAGAGTT GTTCCAATCA GAAAAATCCA TGCTCTGACC 
ATCACATGGG CACTTCCTCC TCAGCAGCAG CACTACAGGG TGAAGCCACT TCATTATATT 
TCCTGGCTTG TTGGACATGA AGGCAAAGGC AGCATTCTTT CTTATCTTAG AAAAAAGTGC 
TGGGCTCTTG CATTGTTTGG TGGAAATGGT GAGACAGGTT TTGAGCAAAA TTCTACTTAC 
TCAGTGTTCA GCATTTCTAT TACGCTGACT GATGAGGGCT ATGAGCATTT TTATGAGGTT 
GCCCACACTG TCTTTCAGTA TTTAAAAATG CTGCAGAAGC TAGGTCCAGA GAAAAGAGTT 
TTTGAAGAGA TTCAGAAAAT TGAGGATAAT GAATTTCATT ATCAAGAACA GACAGATCCA 
GTCGAGTATG TGGAGAACAT GTGTGAGAAC ATGCAGCTGT ACCCACGTCA GGACTTTCTC 
ACCGGAGATC AGCTTCTGTT TGAGTACAAG CCAGAAGTCA TTGCTGAGGC CCTTAATCAG 
CTAGTTCCTC AGAAGGCAAA TCTCGTTCTT CTCTCTGGTG CTAATGAGGG AAGATGTGAC 
CTCAAGGAGA AGTGGTTTGG GACTCAGTAT AGCATAGAAG ATATTGAAAA CTCTTGGACT 
GAACTATGGA AGAGTAATTT TGACTTAAAT CCAGACCTTC ATCTTCCAGC TGAAAATAAG 
TACATAGCCA CGGACTTTAC GTTGAAGGCT TTTGATTGCC CTGAAACTGA ATACCCTGCT 
AAAATTGTCA ATACCGCACA AGGCTGCTTG TGGTATAAGA AAGACAACAA ATTCAAAATC 
CCCAAAGCAT ATATACGATT TCATCTGATC TCACCTTTGA TACAGAAATC TGCAGCAAAT 
GTTGTCCTCT TTGACATCTT CGTGAATATT CTTACGCACA ACCTTGCCGA ACCCGCCTAT 
GAAGCTGATG TGGCACAGCT GGAGTATAAA CTGGTAGCTG GAGAACATGG GTTAATTATT 
CGAGTGAAAG GATTCAACCA CAAACTACCT CTGCTCTTTC AGCTCATTAT TGACTACCTG 
ACTGAGTTCA GTTCCACACC CGCTGTCTTT ACCATGATAA CTGAGCAACT GAAGAAGACC 
TACTTCAACA TCCTCATCAA GCCCGAGACT CTGGCCAAAG ATGTGCGGCT TTTAATTCTG 
GAATATTCCC GCTGGTCTAT GATTGACAAG TACCAAGCTT TGATGGATGG CCTTTCCCTT 
GATTCTCTGC TAAACTTTGT CAAGGATTTT AAATCCCAAC TTTTTGTTGA GGGCCTGGTC 
CAAGGGAATG TCACCAGCAC GGAATCGATG GATTTCCTAA AATATGTTGT TGACAAGCTG 
AACTTCGCGC CCCTGGAGCG GGAGATGCCT GTGCAGTTCC AGGTGGTGGA GCTGCCGAGC 
GGCCACCACC TGTGCAAAGT CAGAGCTCTG AATAAAGGGG ATGCCAACTC TGAAGTCACT 
GTGTACTACC AGTCAGGTAC GAGGAGTCTG AGAGAATATA CTCTTATGGA GCTGCTTGTG 
ATGCACATGG AAGAGCCCTG CTTTGACTTC CTTCGCACCA AGCAGACCCT TGGGTACCAT 
GTCTACCCAA CCTGTAGGAA CACATCTGGA ATTCTAGGAT TTTCTGTAAC TGTTGGGACT 
CAGGCAACCA AATACAACTC CGAAACTGTT GACAAGAAGA TAGAGGAATT TCTTTCCAGC 
TTTGAGGAGA AGATTGAGAA CCTCACAGAA GATGCATTCA ATACTCAGGT CACAGCACTG 
ATCAAGTTGA AAGAGTGTGA GGACACCCAC CTTGGGGAGG AGGTGGACAG GAACTGGAAT 
GAAGTGGTGA CACAGCAGTA TCTCTTTGAC CGCCTTGCCC ATGAGATTGA AGCCCTGAAG 
TCTTTCTCAA AGTCAGACCT GGTCAGCTGG TTCAAGGCTC ACAGAGGACC TGGAAGTAAA 
ATGCTCAGCG TTCATGTTGT TGGATATGGG AAGTATGAAC TGGAAGAGGA TGGTGCCCCT 
TTTGGTGAGG ATTCAAATTC TCGTGAAGGG ATGCAGCTAA CCTACCTGCC ACCCTCTCCT 
GTCCTGGCAG AATCCACCAC CCCCATTACT GACATCAGGG CCTTCACAGC AACACTCAGC 
CTCTTCCCCT ACCATAAAAT AGTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrd1 ( NM_001347169.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334098.1 Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant 1, mRNA.

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CloneID	OMu33518
Clone ID Related Accession (Same CDS sequence)	NM_001347169.1 , NM_001347169.2
Accession Version	NM_001347169.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-20
Organism	Mus musculus(house mouse)
Product	nardilysin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627406.15 and BE949999.1. On Oct 4, 2017 this sequence version replaced NM_001347169.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.238729.1, SRR1660815.262097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGCAATCGC AGAACCTGTT TTTGCTGTGG TCAAAGCTGA CTGATAGACT GTGGTTTAAG 
TCATCTTATT CAAAAATGTC TTCAACCCTG CTGGTCGAGA CAAGAAATCT TTATGGGGTA 
GTTGGAGCTG AAAGCAGGTC TGCACCTGTT GAGCATTTGG CAGGATGGCA AGTGGAGGAG 
CAGCAGGGTG AAACTGACAC AGTGCTGTCT GCAGCGGCTC TGTGTGTTGG AGTTGGGAGC 
TTTGCTGATC CAGATGACCT GCCTGGGCTG GCACACTTTT TGGAGCACAT GGTATTCATG 
GGTAGTTTGA AATACCCTGA TGAGAATGGA TTTGATGCCT TCCTCAAGAA ACATGGAGGT 
AGTGATAATG CCTCAACTGA TTGTGAACGC ACAGTCTTTC AGTTTGATGT CCAGAGAAAG 
TATTTCAAGG AAGCCCTGGA TAGATGGGCC CAGTTCTTTA TCCATCCACT GATGATCAGA 
GATGCAATTG ACCGAGAGGT GGAAGCTGTG GACAGTGAGT ATCAGCTTGC AAGGCCTTCT 
GATGCAAACA GAAAAGAAAT GTTGTTTGGA AGCCTCGCTA GACCGGGACA TCCTATGGGG 
AAGTTTTTTT GGGGAAATGC TGAGACACTC AAGCATGAAC CAAAGAAGAA TAACATTGAT 
ACACATGCCA GACTGAGAGA ATTCTGGATG CGCTACTACT CTGCTCATTA CATGACCTTA 
GTTGTTCAGT CCAAAGAAAC CCTGGACACT TTGGAAAAGT GGGTGACGGA GATCTTCTCT 
CAGATACCAA ACAATGGGCT ACCTAAACCA AACTTTAGCC ATTTAACGGA TCCTTTTGAC 
ACACCAGCAT TTAACAAACT TTATAGAGTT GTTCCAATCA GAAAAATCCA TGCTCTGACC 
ATCACATGGG CACTTCCTCC TCAGCAGCAG CACTACAGGG TGAAGCCACT TCATTATATT 
TCCTGGCTTG TTGGACATGA AGGCAAAGGC AGCATTCTTT CTTATCTTAG AAAAAAGTGC 
TGGGCTCTTG CATTGTTTGG TGGAAATGGT GAGACAGGTT TTGAGCAAAA TTCTACTTAC 
TCAGTGTTCA GCATTTCTAT TACGCTGACT GATGAGGGCT ATGAGCATTT TTATGAGGTT 
GCCCACACTG TCTTTCAGTA TTTAAAAATG CTGCAGAAGC TAGGTCCAGA GAAAAGAGTT 
TTTGAAGAGA TTCAGAAAAT TGAGGATAAT GAATTTCATT ATCAAGAACA GACAGATCCA 
GTCGAGTATG TGGAGAACAT GTGTGAGAAC ATGCAGCTGT ACCCACGTCA GGACTTTCTC 
ACCGGAGATC AGCTTCTGTT TGAGTACAAG CCAGAAGTCA TTGCTGAGGC CCTTAATCAG 
CTAGTTCCTC AGAAGGCAAA TCTCGTTCTT CTCTCTGGTG CTAATGAGGG AAGATGTGAC 
CTCAAGGAGA AGTGGTTTGG GACTCAGTAT AGCATAGAAG ATATTGAAAA CTCTTGGACT 
GAACTATGGA AGAGTAATTT TGACTTAAAT CCAGACCTTC ATCTTCCAGC TGAAAATAAG 
TACATAGCCA CGGACTTTAC GTTGAAGGCT TTTGATTGCC CTGAAACTGA ATACCCTGCT 
AAAATTGTCA ATACCGCACA AGGCTGCTTG TGGTATAAGA AAGACAACAA ATTCAAAATC 
CCCAAAGCAT ATATACGATT TCATCTGATC TCACCTTTGA TACAGAAATC TGCAGCAAAT 
GTTGTCCTCT TTGACATCTT CGTGAATATT CTTACGCACA ACCTTGCCGA ACCCGCCTAT 
GAAGCTGATG TGGCACAGCT GGAGTATAAA CTGGTAGCTG GAGAACATGG GTTAATTATT 
CGAGTGAAAG GATTCAACCA CAAACTACCT CTGCTCTTTC AGCTCATTAT TGACTACCTG 
ACTGAGTTCA GTTCCACACC CGCTGTCTTT ACCATGATAA CTGAGCAACT GAAGAAGACC 
TACTTCAACA TCCTCATCAA GCCCGAGACT CTGGCCAAAG ATGTGCGGCT TTTAATTCTG 
GAATATTCCC GCTGGTCTAT GATTGACAAG TACCAAGCTT TGATGGATGG CCTTTCCCTT 
GATTCTCTGC TAAACTTTGT CAAGGATTTT AAATCCCAAC TTTTTGTTGA GGGCCTGGTC 
CAAGGGAATG TCACCAGCAC GGAATCGATG GATTTCCTAA AATATGTTGT TGACAAGCTG 
AACTTCGCGC CCCTGGAGCG GGAGATGCCT GTGCAGTTCC AGGTGGTGGA GCTGCCGAGC 
GGCCACCACC TGTGCAAAGT CAGAGCTCTG AATAAAGGGG ATGCCAACTC TGAAGTCACT 
GTGTACTACC AGTCAGGTAC GAGGAGTCTG AGAGAATATA CTCTTATGGA GCTGCTTGTG 
ATGCACATGG AAGAGCCCTG CTTTGACTTC CTTCGCACCA AGCAGACCCT TGGGTACCAT 
GTCTACCCAA CCTGTAGGAA CACATCTGGA ATTCTAGGAT TTTCTGTAAC TGTTGGGACT 
CAGGCAACCA AATACAACTC CGAAACTGTT GACAAGAAGA TAGAGGAATT TCTTTCCAGC 
TTTGAGGAGA AGATTGAGAA CCTCACAGAA GATGCATTCA ATACTCAGGT CACAGCACTG 
ATCAAGTTGA AAGAGTGTGA GGACACCCAC CTTGGGGAGG AGGTGGACAG GAACTGGAAT 
GAAGTGGTGA CACAGCAGTA TCTCTTTGAC CGCCTTGCCC ATGAGATTGA AGCCCTGAAG 
TCTTTCTCAA AGTCAGACCT GGTCAGCTGG TTCAAGGCTC ACAGAGGACC TGGAAGTAAA 
ATGCTCAGCG TTCATGTTGT TGGATATGGG AAGTATGAAC TGGAAGAGGA TGGTGCCCCT 
TTTGGTGAGG ATTCAAATTC TCGTGAAGGG ATGCAGCTAA CCTACCTGCC ACCCTCTCCT 
GTCCTGGCAG AATCCACCAC CCCCATTACT GACATCAGGG CCTTCACAGC AACACTCAGC 
CTCTTCCCCT ACCATAAAAT AGTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334098.1
CDS	179..3868
Translation	MLRRVAVAAVCVTGRKLRCEAGRELTALGRIEARGLCEESSKPFPTLTMPGRNKAKSTCSCPDLQPNGQDLGESGRLARLGADESEEEGRSFSNVGDPEIIKSPSDPKQYRYIKLQNGLQALLISDLSNVEGKTGNATDEEEEEEEEEEEEDDDDDDDDDDDDEDSGAEIQDDDEEGFDDEEEFDDDDDDEHDDDDLENEENELEELEERVEARKKTTEKQQSQNLFLLWSKLTDRLWFKSSYSKMSSTLLVETRNLYGVVGAESRSAPVEHLAGWQVEEQQGETDTVLSAAALCVGVGSFADPDDLPGLAHFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKYFKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLKHEPKKNNIDTHARLREFWMRYYSAHYMTLVVQSKETLDTLEKWVTEIFSQIPNNGLPKPNFSHLTDPFDTPAFNKLYRVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGSILSYLRKKCWALALFGGNGETGFEQNSTYSVFSISITLTDEGYEHFYEVAHTVFQYLKMLQKLGPEKRVFEEIQKIEDNEFHYQEQTDPVEYVENMCENMQLYPRQDFLTGDQLLFEYKPEVIAEALNQLVPQKANLVLLSGANEGRCDLKEKWFGTQYSIEDIENSWTELWKSNFDLNPDLHLPAENKYIATDFTLKAFDCPETEYPAKIVNTAQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNHKLPLLFQLIIDYLTEFSSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYSRWSMIDKYQALMDGLSLDSLLNFVKDFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFAPLEREMPVQFQVVELPSGHHLCKVRALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGYHVYPTCRNTSGILGFSVTVGTQATKYNSETVDKKIEEFLSSFEEKIENLTEDAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFDRLAHEIEALKSFSKSDLVSWFKAHRGPGSKMLSVHVVGYGKYELEEDGAPFGEDSNSREGMQLTYLPPSPVLAESTTPITDIRAFTATLSLFPYHKIVK

Target ORF information:

RefSeq Version	NM_001347169.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347169.2

ORF Insert Sequence:

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGCAATCGC AGAACCTGTT TTTGCTGTGG TCAAAGCTGA CTGATAGACT GTGGTTTAAG 
TCATCTTATT CAAAAATGTC TTCAACCCTG CTGGTCGAGA CAAGAAATCT TTATGGGGTA 
GTTGGAGCTG AAAGCAGGTC TGCACCTGTT GAGCATTTGG CAGGATGGCA AGTGGAGGAG 
CAGCAGGGTG AAACTGACAC AGTGCTGTCT GCAGCGGCTC TGTGTGTTGG AGTTGGGAGC 
TTTGCTGATC CAGATGACCT GCCTGGGCTG GCACACTTTT TGGAGCACAT GGTATTCATG 
GGTAGTTTGA AATACCCTGA TGAGAATGGA TTTGATGCCT TCCTCAAGAA ACATGGAGGT 
AGTGATAATG CCTCAACTGA TTGTGAACGC ACAGTCTTTC AGTTTGATGT CCAGAGAAAG 
TATTTCAAGG AAGCCCTGGA TAGATGGGCC CAGTTCTTTA TCCATCCACT GATGATCAGA 
GATGCAATTG ACCGAGAGGT GGAAGCTGTG GACAGTGAGT ATCAGCTTGC AAGGCCTTCT 
GATGCAAACA GAAAAGAAAT GTTGTTTGGA AGCCTCGCTA GACCGGGACA TCCTATGGGG 
AAGTTTTTTT GGGGAAATGC TGAGACACTC AAGCATGAAC CAAAGAAGAA TAACATTGAT 
ACACATGCCA GACTGAGAGA ATTCTGGATG CGCTACTACT CTGCTCATTA CATGACCTTA 
GTTGTTCAGT CCAAAGAAAC CCTGGACACT TTGGAAAAGT GGGTGACGGA GATCTTCTCT 
CAGATACCAA ACAATGGGCT ACCTAAACCA AACTTTAGCC ATTTAACGGA TCCTTTTGAC 
ACACCAGCAT TTAACAAACT TTATAGAGTT GTTCCAATCA GAAAAATCCA TGCTCTGACC 
ATCACATGGG CACTTCCTCC TCAGCAGCAG CACTACAGGG TGAAGCCACT TCATTATATT 
TCCTGGCTTG TTGGACATGA AGGCAAAGGC AGCATTCTTT CTTATCTTAG AAAAAAGTGC 
TGGGCTCTTG CATTGTTTGG TGGAAATGGT GAGACAGGTT TTGAGCAAAA TTCTACTTAC 
TCAGTGTTCA GCATTTCTAT TACGCTGACT GATGAGGGCT ATGAGCATTT TTATGAGGTT 
GCCCACACTG TCTTTCAGTA TTTAAAAATG CTGCAGAAGC TAGGTCCAGA GAAAAGAGTT 
TTTGAAGAGA TTCAGAAAAT TGAGGATAAT GAATTTCATT ATCAAGAACA GACAGATCCA 
GTCGAGTATG TGGAGAACAT GTGTGAGAAC ATGCAGCTGT ACCCACGTCA GGACTTTCTC 
ACCGGAGATC AGCTTCTGTT TGAGTACAAG CCAGAAGTCA TTGCTGAGGC CCTTAATCAG 
CTAGTTCCTC AGAAGGCAAA TCTCGTTCTT CTCTCTGGTG CTAATGAGGG AAGATGTGAC 
CTCAAGGAGA AGTGGTTTGG GACTCAGTAT AGCATAGAAG ATATTGAAAA CTCTTGGACT 
GAACTATGGA AGAGTAATTT TGACTTAAAT CCAGACCTTC ATCTTCCAGC TGAAAATAAG 
TACATAGCCA CGGACTTTAC GTTGAAGGCT TTTGATTGCC CTGAAACTGA ATACCCTGCT 
AAAATTGTCA ATACCGCACA AGGCTGCTTG TGGTATAAGA AAGACAACAA ATTCAAAATC 
CCCAAAGCAT ATATACGATT TCATCTGATC TCACCTTTGA TACAGAAATC TGCAGCAAAT 
GTTGTCCTCT TTGACATCTT CGTGAATATT CTTACGCACA ACCTTGCCGA ACCCGCCTAT 
GAAGCTGATG TGGCACAGCT GGAGTATAAA CTGGTAGCTG GAGAACATGG GTTAATTATT 
CGAGTGAAAG GATTCAACCA CAAACTACCT CTGCTCTTTC AGCTCATTAT TGACTACCTG 
ACTGAGTTCA GTTCCACACC CGCTGTCTTT ACCATGATAA CTGAGCAACT GAAGAAGACC 
TACTTCAACA TCCTCATCAA GCCCGAGACT CTGGCCAAAG ATGTGCGGCT TTTAATTCTG 
GAATATTCCC GCTGGTCTAT GATTGACAAG TACCAAGCTT TGATGGATGG CCTTTCCCTT 
GATTCTCTGC TAAACTTTGT CAAGGATTTT AAATCCCAAC TTTTTGTTGA GGGCCTGGTC 
CAAGGGAATG TCACCAGCAC GGAATCGATG GATTTCCTAA AATATGTTGT TGACAAGCTG 
AACTTCGCGC CCCTGGAGCG GGAGATGCCT GTGCAGTTCC AGGTGGTGGA GCTGCCGAGC 
GGCCACCACC TGTGCAAAGT CAGAGCTCTG AATAAAGGGG ATGCCAACTC TGAAGTCACT 
GTGTACTACC AGTCAGGTAC GAGGAGTCTG AGAGAATATA CTCTTATGGA GCTGCTTGTG 
ATGCACATGG AAGAGCCCTG CTTTGACTTC CTTCGCACCA AGCAGACCCT TGGGTACCAT 
GTCTACCCAA CCTGTAGGAA CACATCTGGA ATTCTAGGAT TTTCTGTAAC TGTTGGGACT 
CAGGCAACCA AATACAACTC CGAAACTGTT GACAAGAAGA TAGAGGAATT TCTTTCCAGC 
TTTGAGGAGA AGATTGAGAA CCTCACAGAA GATGCATTCA ATACTCAGGT CACAGCACTG 
ATCAAGTTGA AAGAGTGTGA GGACACCCAC CTTGGGGAGG AGGTGGACAG GAACTGGAAT 
GAAGTGGTGA CACAGCAGTA TCTCTTTGAC CGCCTTGCCC ATGAGATTGA AGCCCTGAAG 
TCTTTCTCAA AGTCAGACCT GGTCAGCTGG TTCAAGGCTC ACAGAGGACC TGGAAGTAAA 
ATGCTCAGCG TTCATGTTGT TGGATATGGG AAGTATGAAC TGGAAGAGGA TGGTGCCCCT 
TTTGGTGAGG ATTCAAATTC TCGTGAAGGG ATGCAGCTAA CCTACCTGCC ACCCTCTCCT 
GTCCTGGCAG AATCCACCAC CCCCATTACT GACATCAGGG CCTTCACAGC AACACTCAGC 
CTCTTCCCCT ACCATAAAAT AGTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrd1 ( NM_001346554.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001333483.1 Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant 3, mRNA.

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CloneID	OMu83486
Clone ID Related Accession (Same CDS sequence)	NM_001346554.1
Accession Version	NM_001346554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	nardilysin isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627406.15 and BE949999.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK166470.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGTCTGCAG CGGCTCTGTG TGTTGGAGTT GGGAGCTTTG CTGATCCAGA TGACCTGCCT 
GGGCTGGCAC ACTTTTTGGA GCACATGGTA TTCATGGGTA GTTTGAAATA CCCTGATGAG 
AATGGATTTG ATGCCTTCCT CAAGAAACAT GGAGGTAGTG ATAATGCCTC AACTGATTGT 
GAACGCACAG TCTTTCAGTT TGATGTCCAG AGAAAGTATT TCAAGGAAGC CCTGGATAGA 
TGGGCCCAGT TCTTTATCCA TCCACTGATG ATCAGAGATG CAATTGACCG AGAGGTGGAA 
GCTGTGGACA GTGAGTATCA GCTTGCAAGG CCTTCTGATG CAAACAGAAA AGAAATGTTG 
TTTGGAAGCC TCGCTAGACC GGGACATCCT ATGGGGAAGT TTTTTTGGGG AAATGCTGAG 
ACACTCAAGC ATGAACCAAA GAAGAATAAC ATTGATACAC ATGCCAGACT GAGAGAATTC 
TGGATGCGCT ACTACTCTGC TCATTACATG ACCTTAGTTG TTCAGTCCAA AGTTGTTCCA 
ATCAGAAAAA TCCATGCTCT GACCATCACA TGGGCACTTC CTCCTCAGCA GCAGCACTAC 
AGGGTGAAGC CACTTCATTA TATTTCCTGG CTTGTTGGAC ATGAAGGCAA AGGCAGCATT 
CTTTCTTATC TTAGAAAAAA GTGCTGGGCT CTTGCATTGT TTGGTGGAAA TGGTGAGACA 
GGTTTTGAGC AAAATTCTAC TTACTCAGTG TTCAGCATTT CTATTACGCT GACTGATGAG 
GGCTATGAGC ATTTTTATGA GGTTGCCCAC ACTGTCTTTC AGTATTTAAA AATGCTGCAG 
AAGCTAGGTC CAGAGAAAAG AGTTTTTGAA GAGATTCAGA AAATTGAGGA TAATGAATTT 
CATTATCAAG AACAGACAGA TCCAGTCGAG TATGTGGAGA ACATGTGTGA GAACATGCAG 
CTGTACCCAC GTCAGGACTT TCTCACCGGA GATCAGCTTC TGTTTGAGTA CAAGCCAGAA 
GTCATTGCTG AGGCCCTTAA TCAGCTAGTT CCTCAGAAGG CAAATCTCGT TCTTCTCTCT 
GGTGCTAATG AGGGAAGATG TGACCTCAAG GAGAAGTGGT TTGGGACTCA GTATAGCATA 
GAAGATATTG AAAACTCTTG GACTGAACTA TGGAAGAGTA ATTTTGACTT AAATCCAGAC 
CTTCATCTTC CAGCTGAAAA TAAGTACATA GCCACGGACT TTACGTTGAA GGCTTTTGAT 
TGCCCTGAAA CTGAATACCC TGCTAAAATT GTCAATACCG CACAAGGCTG CTTGTGGTAT 
AAGAAAGACA ACAAATTCAA AATCCCCAAA GCATATATAC GATTTCATCT GATCTCACCT 
TTGATACAGA AATCTGCAGC AAATGTTGTC CTCTTTGACA TCTTCGTGAA TATTCTTACG 
CACAACCTTG CCGAACCCGC CTATGAAGCT GATGTGGCAC AGCTGGAGTA TAAACTGGTA 
GCTGGAGAAC ATGGGTTAAT TATTCGAGTG AAAGGATTCA ACCACAAACT ACCTCTGCTC 
TTTCAGCTCA TTATTGACTA CCTGACTGAG TTCAGTTCCA CACCCGCTGT CTTTACCATG 
ATAACTGAGC AACTGAAGAA GACCTACTTC AACATCCTCA TCAAGCCCGA GACTCTGGCC 
AAAGATGTGC GGCTTTTAAT TCTGGAATAT TCCCGCTGGT CTATGATTGA CAAGTACCAA 
GCTTTGATGG ATGGCCTTTC CCTTGATTCT CTGCTAAACT TTGTCAAGGA TTTTAAATCC 
CAACTTTTTG TTGAGGGCCT GGTCCAAGGG AATGTCACCA GCACGGAATC GATGGATTTC 
CTAAAATATG TTGTTGACAA GCTGAACTTC GCGCCCCTGG AGCGGGAGAT GCCTGTGCAG 
TTCCAGGTGG TGGAGCTGCC GAGCGGCCAC CACCTGTGCA AAGTCAGAGC TCTGAATAAA 
GGGGATGCCA ACTCTGAAGT CACTGTGTAC TACCAGTCAG GTACGAGGAG TCTGAGAGAA 
TATACTCTTA TGGAGCTGCT TGTGATGCAC ATGGAAGAGC CCTGCTTTGA CTTCCTTCGC 
ACCAAGCAGA CCCTTGGGTA CCATGTCTAC CCAACCTGTA GGAACACATC TGGAATTCTA 
GGATTTTCTG TAACTGTTGG GACTCAGGCA ACCAAATACA ACTCCGAAAC TGTTGACAAG 
AAGATAGAGG AATTTCTTTC CAGCTTTGAG GAGAAGATTG AGAACCTCAC AGAAGATGCA 
TTCAATACTC AGGTCACAGC ACTGATCAAG TTGAAAGAGT GTGAGGACAC CCACCTTGGG 
GAGGAGGTGG ACAGGAACTG GAATGAAGTG GTGACACAGC AGTATCTCTT TGACCGCCTT 
GCCCATGAGA TTGAAGCCCT GAAGTCTTTC TCAAAGTCAG ACCTGGTCAG CTGGTTCAAG 
GCTCACAGAG GACCTGGAAG TAAAATGCTC AGCGTTCATG TTGTTGGATA TGGGAAGTAT 
GAACTGGAAG AGGATGGTGC CCCTTTTGGT GAGGATTCAA ATTCTCGTGA AGGGATGCAG 
CTAACCTACC TGCCACCCTC TCCTGTCCTG GCAGAATCCA CCACCCCCAT TACTGACATC 
AGGGCCTTCA CAGCAACACT CAGCCTCTTC CCCTACCATA AAATAGTCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333483.1
CDS	179..3532
Translation	MLRRVAVAAVCVTGRKLRCEAGRELTALGRIEARGLCEESSKPFPTLTMPGRNKAKSTCSCPDLQPNGQDLGESGRLARLGADESEEEGRSFSNVGDPEIIKSPSDPKQYRYIKLQNGLQALLISDLSNVEGKTGNATDEEEEEEEEEEEEDDDDDDDDDDDDEDSGAEIQDDDEEGFDDEEEFDDDDDDEHDDDDLENEENELEELEERVEARKKTTEKQSAAALCVGVGSFADPDDLPGLAHFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKYFKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLKHEPKKNNIDTHARLREFWMRYYSAHYMTLVVQSKVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGSILSYLRKKCWALALFGGNGETGFEQNSTYSVFSISITLTDEGYEHFYEVAHTVFQYLKMLQKLGPEKRVFEEIQKIEDNEFHYQEQTDPVEYVENMCENMQLYPRQDFLTGDQLLFEYKPEVIAEALNQLVPQKANLVLLSGANEGRCDLKEKWFGTQYSIEDIENSWTELWKSNFDLNPDLHLPAENKYIATDFTLKAFDCPETEYPAKIVNTAQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNHKLPLLFQLIIDYLTEFSSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYSRWSMIDKYQALMDGLSLDSLLNFVKDFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFAPLEREMPVQFQVVELPSGHHLCKVRALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGYHVYPTCRNTSGILGFSVTVGTQATKYNSETVDKKIEEFLSSFEEKIENLTEDAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFDRLAHEIEALKSFSKSDLVSWFKAHRGPGSKMLSVHVVGYGKYELEEDGAPFGEDSNSREGMQLTYLPPSPVLAESTTPITDIRAFTATLSLFPYHKIVK

Target ORF information:

RefSeq Version	NM_001346554.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346554.1

ORF Insert Sequence:

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGTCTGCAG CGGCTCTGTG TGTTGGAGTT GGGAGCTTTG CTGATCCAGA TGACCTGCCT 
GGGCTGGCAC ACTTTTTGGA GCACATGGTA TTCATGGGTA GTTTGAAATA CCCTGATGAG 
AATGGATTTG ATGCCTTCCT CAAGAAACAT GGAGGTAGTG ATAATGCCTC AACTGATTGT 
GAACGCACAG TCTTTCAGTT TGATGTCCAG AGAAAGTATT TCAAGGAAGC CCTGGATAGA 
TGGGCCCAGT TCTTTATCCA TCCACTGATG ATCAGAGATG CAATTGACCG AGAGGTGGAA 
GCTGTGGACA GTGAGTATCA GCTTGCAAGG CCTTCTGATG CAAACAGAAA AGAAATGTTG 
TTTGGAAGCC TCGCTAGACC GGGACATCCT ATGGGGAAGT TTTTTTGGGG AAATGCTGAG 
ACACTCAAGC ATGAACCAAA GAAGAATAAC ATTGATACAC ATGCCAGACT GAGAGAATTC 
TGGATGCGCT ACTACTCTGC TCATTACATG ACCTTAGTTG TTCAGTCCAA AGTTGTTCCA 
ATCAGAAAAA TCCATGCTCT GACCATCACA TGGGCACTTC CTCCTCAGCA GCAGCACTAC 
AGGGTGAAGC CACTTCATTA TATTTCCTGG CTTGTTGGAC ATGAAGGCAA AGGCAGCATT 
CTTTCTTATC TTAGAAAAAA GTGCTGGGCT CTTGCATTGT TTGGTGGAAA TGGTGAGACA 
GGTTTTGAGC AAAATTCTAC TTACTCAGTG TTCAGCATTT CTATTACGCT GACTGATGAG 
GGCTATGAGC ATTTTTATGA GGTTGCCCAC ACTGTCTTTC AGTATTTAAA AATGCTGCAG 
AAGCTAGGTC CAGAGAAAAG AGTTTTTGAA GAGATTCAGA AAATTGAGGA TAATGAATTT 
CATTATCAAG AACAGACAGA TCCAGTCGAG TATGTGGAGA ACATGTGTGA GAACATGCAG 
CTGTACCCAC GTCAGGACTT TCTCACCGGA GATCAGCTTC TGTTTGAGTA CAAGCCAGAA 
GTCATTGCTG AGGCCCTTAA TCAGCTAGTT CCTCAGAAGG CAAATCTCGT TCTTCTCTCT 
GGTGCTAATG AGGGAAGATG TGACCTCAAG GAGAAGTGGT TTGGGACTCA GTATAGCATA 
GAAGATATTG AAAACTCTTG GACTGAACTA TGGAAGAGTA ATTTTGACTT AAATCCAGAC 
CTTCATCTTC CAGCTGAAAA TAAGTACATA GCCACGGACT TTACGTTGAA GGCTTTTGAT 
TGCCCTGAAA CTGAATACCC TGCTAAAATT GTCAATACCG CACAAGGCTG CTTGTGGTAT 
AAGAAAGACA ACAAATTCAA AATCCCCAAA GCATATATAC GATTTCATCT GATCTCACCT 
TTGATACAGA AATCTGCAGC AAATGTTGTC CTCTTTGACA TCTTCGTGAA TATTCTTACG 
CACAACCTTG CCGAACCCGC CTATGAAGCT GATGTGGCAC AGCTGGAGTA TAAACTGGTA 
GCTGGAGAAC ATGGGTTAAT TATTCGAGTG AAAGGATTCA ACCACAAACT ACCTCTGCTC 
TTTCAGCTCA TTATTGACTA CCTGACTGAG TTCAGTTCCA CACCCGCTGT CTTTACCATG 
ATAACTGAGC AACTGAAGAA GACCTACTTC AACATCCTCA TCAAGCCCGA GACTCTGGCC 
AAAGATGTGC GGCTTTTAAT TCTGGAATAT TCCCGCTGGT CTATGATTGA CAAGTACCAA 
GCTTTGATGG ATGGCCTTTC CCTTGATTCT CTGCTAAACT TTGTCAAGGA TTTTAAATCC 
CAACTTTTTG TTGAGGGCCT GGTCCAAGGG AATGTCACCA GCACGGAATC GATGGATTTC 
CTAAAATATG TTGTTGACAA GCTGAACTTC GCGCCCCTGG AGCGGGAGAT GCCTGTGCAG 
TTCCAGGTGG TGGAGCTGCC GAGCGGCCAC CACCTGTGCA AAGTCAGAGC TCTGAATAAA 
GGGGATGCCA ACTCTGAAGT CACTGTGTAC TACCAGTCAG GTACGAGGAG TCTGAGAGAA 
TATACTCTTA TGGAGCTGCT TGTGATGCAC ATGGAAGAGC CCTGCTTTGA CTTCCTTCGC 
ACCAAGCAGA CCCTTGGGTA CCATGTCTAC CCAACCTGTA GGAACACATC TGGAATTCTA 
GGATTTTCTG TAACTGTTGG GACTCAGGCA ACCAAATACA ACTCCGAAAC TGTTGACAAG 
AAGATAGAGG AATTTCTTTC CAGCTTTGAG GAGAAGATTG AGAACCTCAC AGAAGATGCA 
TTCAATACTC AGGTCACAGC ACTGATCAAG TTGAAAGAGT GTGAGGACAC CCACCTTGGG 
GAGGAGGTGG ACAGGAACTG GAATGAAGTG GTGACACAGC AGTATCTCTT TGACCGCCTT 
GCCCATGAGA TTGAAGCCCT GAAGTCTTTC TCAAAGTCAG ACCTGGTCAG CTGGTTCAAG 
GCTCACAGAG GACCTGGAAG TAAAATGCTC AGCGTTCATG TTGTTGGATA TGGGAAGTAT 
GAACTGGAAG AGGATGGTGC CCCTTTTGGT GAGGATTCAA ATTCTCGTGA AGGGATGCAG 
CTAACCTACC TGCCACCCTC TCCTGTCCTG GCAGAATCCA CCACCCCCAT TACTGACATC 
AGGGCCTTCA CAGCAACACT CAGCCTCTTC CCCTACCATA AAATAGTCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrd1 ( XM_006502991.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503054.1 Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant X2, mRNA.

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CloneID	OMu33519
Clone ID Related Accession (Same CDS sequence)	XM_006502991.3
Accession Version	XM_006502991.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nardilysin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502991.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGCAATCGC AGAACCTGTT TTTGCTGTGG TCAAAGCTGA CTGATAGACT GTGGTTTAAG 
TCATCTTATT CAAAAATGTC TTCAACCCTG CTGGTCGAGA CAAGAAATCT TTATGGGGTA 
GTTGGAGCTG AAAGCAGGTC TGCACCTGTT GAGCATTTGG CAGGATGGCA AGTGGAGGAG 
CAGCAGGGTG AAACTGACAC AGTGCTGTCT GCAGCGGCTC TGTGTGTTGG AGTTGGGAGC 
TTTGCTGATC CAGATGACCT GCCTGGGCTG GCACACTTTT TGGAGCACAT GGTATTCATG 
GGTAGTTTGA AATACCCTGA TGAGAATGGA TTTGATGCCT TCCTCAAGAA ACATGGAGGT 
AGTGATAATG CCTCAACTGA TTGTGAACGC ACAGTCTTTC AGTTTGATGT CCAGAGAAAG 
TATTTCAAGG AAGCCCTGGA TAGATGGGCC CAGTTCTTTA TCCATCCACT GATGATCAGA 
GATGCAATTG ACCGAGAGGT GGAAGCTGTG GACAGTGAGT ATCAGCTTGC AAGGCCTTCT 
GATGCAAACA GAAAAGAAAT GTTGTTTGGA AGCCTCGCTA GACCGGGACA TCCTATGGGG 
AAGTTTTTTT GGGGAAATGC TGAGACACTC AAGCATGAAC CAAAGAAGAA TAACATTGAT 
ACACATGCCA GACTGAGAGA ATTCTGGATG CGCTACTACT CTGCTCATTA CATGACCTTA 
GTTGTTCAGT CCAAAGAAAC CCTGGACACT TTGGAAAAGT GGGTGACGGA GATCTTCTCT 
CAGATACCAA ACAATGGGCT ACCTAAACCA AACTTTAGCC ATTTAACGGA TCCTTTTGAC 
ACACCAGCAT TTAACAAACT TTATAGAGTT GTTCCAATCA GAAAAATCCA TGCTCTGACC 
ATCACATGGG CACTTCCTCC TCAGCAGCAG CACTACAGGG TGAAGCCACT TCATTATATT 
TCCTGGCTTG TTGGACATGA AGGCAAAGGC AGCATTCTTT CTTATCTTAG AAAAAAGTGC 
TGGGCTCTTG CATTGTTTGG TGGAAATGGT GAGACAGGTT TTGAGCAAAA TTCTACTTAC 
TCAGTGTTCA GCATTTCTAT TACGCTGACT GATGAGGGCT ATGAGCATTT TTATGAGGTT 
GCCCACACTG TCTTTCAGTA TTTAAAAATG CTGCAGAAGC TAGGTCCAGA GAAAAGAGTT 
TTTGAAGAGA TTCAGAAAAT TGAGGATAAT GAATTTCATT ATCAAGAACA GACAGATCCA 
GTCGAGTATG TGGAGAACAT GTGTGAGAAC ATGCAGCTGT ACCCACGTCA GGACTTTCTC 
ACCGGAGATC AGCTTCTGTT TGAGTACAAG CCAGAAGTCA TTGCTGAGGC CCTTAATCAG 
CTAGTTCCTC AGAAGGCAAA TCTCGTTCTT CTCTCTGGTG CTAATGAGGG AAGATGTGAC 
CTCAAGGAGA AGTGGTTTGG GACTCAGTAT AGCATAGAAG ATATTGAAAA CTCTTGGACT 
GAACTATGGA AGAGTAATTT TGACTTAAAT CCAGACCTTC ATCTTCCAGC TGAAAATAAG 
TACATAGCCA CGGACTTTAC GTTGAAGGCT TTTGATTGCC CTGAAACTGA ATACCCTGCT 
AAAATTGTCA ATACCGCACA AGGCTGCTTG TGGTATAAGA AAGACAACAA ATTCAAAATC 
CCCAAAGCAT ATATACGATT TCATCTGATC TCACCTTTGA TACAGAAATC TGCAGCAAAT 
GTTGTCCTCT TTGACATCTT CGTGAATATT CTTACGCACA ACCTTGCCGA ACCCGCCTAT 
GAAGCTGATG TGGCACAGCT GGAGTATAAA CTGGTAGCTG GAGAACATGG GTTAATTATT 
CGAGTGAAAG GATTCAACCA CAAACTACCT CTGCTCTTTC AGCTCATTAT TGACTACCTG 
ACTGAGTTCA GTTCCACACC CGCTGTCTTT ACCATGATAA CTGAGCAACT GAAGAAGACC 
TACTTCAACA TCCTCATCAA GCCCGAGACT CTGGCCAAAG ATGTGCGGCT TTTAATTCTG 
GAATATTCCC GCTGGTCTAT GATTGACAAG TACCAAGCTT TGATGGATGG CCTTTCCCTT 
GATTCTCTGC TAAACTTTGT CAAGGATTTT AAATCCCAAC TTTTTGTTGA GGGCCTGGTC 
CAAGGGAATG TCACCAGCAC GGAATCGATG GATTTCCTAA AATATGTTGT TGACAAGCTG 
AACTTCGCGC CCCTGGAGCG GGAGATGCCT GTGCAGTTCC AGGTGGTGGA GCTGCCGAGC 
GGCCACCACC TGTGCAAAGT CAGAGCTCTG AATAAAGGGG ATGCCAACTC TGAAGTCACT 
GTGTACTACC AGATGCACAT GGAAGAGCCC TGCTTTGACT TCCTTCGCAC CAAGCAGACC 
CTTGGGTACC ATGTCTACCC AACCTGTAGG AACACATCTG GAATTCTAGG ATTTTCTGTA 
ACTGTTGGGA CTCAGGCAAC CAAATACAAC TCCGAAACTG TTGACAAGAA GATAGAGGAA 
TTTCTTTCCA GCTTTGAGGA GAAGATTGAG AACCTCACAG AAGATGCATT CAATACTCAG 
GTCACAGCAC TGATCAAGTT GAAAGAGTGT GAGGACACCC ACCTTGGGGA GGAGGTGGAC 
AGGAACTGGA ATGAAGTGGT GACACAGCAG TATCTCTTTG ACCGCCTTGC CCATGAGATT 
GAAGCCCTGA AGTCTTTCTC AAAGTCAGAC CTGGTCAGCT GGTTCAAGGC TCACAGAGGA 
CCTGGAAGTA AAATGCTCAG CGTTCATGTT GTTGGATATG GGAAGTATGA ACTGGAAGAG 
GATGGTGCCC CTTTTGGTGA GGATTCAAAT TCTCGTGAAG GGATGCAGCT AACCTACCTG 
CCACCCTCTC CTGTCCTGGC AGAATCCACC ACCCCCATTA CTGACATCAG GGCCTTCACA 
GCAACACTCA GCCTCTTCCC CTACCATAAA ATAGTCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503054.1
CDS	218..3859
Translation	MLRRVAVAAVCVTGRKLRCEAGRELTALGRIEARGLCEESSKPFPTLTMPGRNKAKSTCSCPDLQPNGQDLGESGRLARLGADESEEEGRSFSNVGDPEIIKSPSDPKQYRYIKLQNGLQALLISDLSNVEGKTGNATDEEEEEEEEEEEEDDDDDDDDDDDDEDSGAEIQDDDEEGFDDEEEFDDDDDDEHDDDDLENEENELEELEERVEARKKTTEKQQSQNLFLLWSKLTDRLWFKSSYSKMSSTLLVETRNLYGVVGAESRSAPVEHLAGWQVEEQQGETDTVLSAAALCVGVGSFADPDDLPGLAHFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKYFKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLKHEPKKNNIDTHARLREFWMRYYSAHYMTLVVQSKETLDTLEKWVTEIFSQIPNNGLPKPNFSHLTDPFDTPAFNKLYRVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGSILSYLRKKCWALALFGGNGETGFEQNSTYSVFSISITLTDEGYEHFYEVAHTVFQYLKMLQKLGPEKRVFEEIQKIEDNEFHYQEQTDPVEYVENMCENMQLYPRQDFLTGDQLLFEYKPEVIAEALNQLVPQKANLVLLSGANEGRCDLKEKWFGTQYSIEDIENSWTELWKSNFDLNPDLHLPAENKYIATDFTLKAFDCPETEYPAKIVNTAQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNHKLPLLFQLIIDYLTEFSSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYSRWSMIDKYQALMDGLSLDSLLNFVKDFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFAPLEREMPVQFQVVELPSGHHLCKVRALNKGDANSEVTVYYQMHMEEPCFDFLRTKQTLGYHVYPTCRNTSGILGFSVTVGTQATKYNSETVDKKIEEFLSSFEEKIENLTEDAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFDRLAHEIEALKSFSKSDLVSWFKAHRGPGSKMLSVHVVGYGKYELEEDGAPFGEDSNSREGMQLTYLPPSPVLAESTTPITDIRAFTATLSLFPYHKIVK

Target ORF information:

RefSeq Version	XM_006502991.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nardilysin, N-arginine dibasic convertase, NRD convertase 1 (Nrd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502991.3

ORF Insert Sequence:

ATGCTGAGGA GAGTCGCGGT TGCTGCAGTC TGTGTCACCG GGAGGAAGTT GCGGTGTGAG 
GCCGGGCGGG AGCTCACGGC TCTGGGGCGA ATCGAAGCAC GGGGTCTGTG CGAAGAATCA 
TCGAAACCCT TTCCTACGCT GACCATGCCT GGAAGAAACA AGGCGAAGTC CACCTGCAGT 
TGCCCGGACC TGCAGCCCAA TGGACAGGAC TTGGGCGAGA GCGGCCGGCT TGCTCGTCTA 
GGAGCGGATG AATCTGAGGA GGAGGGACGG TCTTTCAGTA ATGTCGGGGA CCCTGAGATC 
ATCAAGTCTC CCAGCGATCC CAAGCAGTAC CGATACATCA AATTACAGAA TGGCTTGCAG 
GCACTTTTGA TTTCAGATCT AAGTAATGTG GAAGGTAAGA CAGGAAATGC AACAGATGAA 
GAGGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGATGATG ATGATGACGA CGACGACGAC 
GATGATGATG AAGACTCTGG AGCTGAGATA CAAGATGATG ATGAGGAAGG TTTTGATGAT 
GAAGAGGAAT TTGATGATGA CGATGATGAT GAACATGATG ATGATGATCT TGAGAATGAG 
GAAAACGAAC TGGAAGAGTT GGAAGAGAGG GTAGAAGCCA GGAAGAAAAC TACTGAGAAA 
CAGCAATCGC AGAACCTGTT TTTGCTGTGG TCAAAGCTGA CTGATAGACT GTGGTTTAAG 
TCATCTTATT CAAAAATGTC TTCAACCCTG CTGGTCGAGA CAAGAAATCT TTATGGGGTA 
GTTGGAGCTG AAAGCAGGTC TGCACCTGTT GAGCATTTGG CAGGATGGCA AGTGGAGGAG 
CAGCAGGGTG AAACTGACAC AGTGCTGTCT GCAGCGGCTC TGTGTGTTGG AGTTGGGAGC 
TTTGCTGATC CAGATGACCT GCCTGGGCTG GCACACTTTT TGGAGCACAT GGTATTCATG 
GGTAGTTTGA AATACCCTGA TGAGAATGGA TTTGATGCCT TCCTCAAGAA ACATGGAGGT 
AGTGATAATG CCTCAACTGA TTGTGAACGC ACAGTCTTTC AGTTTGATGT CCAGAGAAAG 
TATTTCAAGG AAGCCCTGGA TAGATGGGCC CAGTTCTTTA TCCATCCACT GATGATCAGA 
GATGCAATTG ACCGAGAGGT GGAAGCTGTG GACAGTGAGT ATCAGCTTGC AAGGCCTTCT 
GATGCAAACA GAAAAGAAAT GTTGTTTGGA AGCCTCGCTA GACCGGGACA TCCTATGGGG 
AAGTTTTTTT GGGGAAATGC TGAGACACTC AAGCATGAAC CAAAGAAGAA TAACATTGAT 
ACACATGCCA GACTGAGAGA ATTCTGGATG CGCTACTACT CTGCTCATTA CATGACCTTA 
GTTGTTCAGT CCAAAGAAAC CCTGGACACT TTGGAAAAGT GGGTGACGGA GATCTTCTCT 
CAGATACCAA ACAATGGGCT ACCTAAACCA AACTTTAGCC ATTTAACGGA TCCTTTTGAC 
ACACCAGCAT TTAACAAACT TTATAGAGTT GTTCCAATCA GAAAAATCCA TGCTCTGACC 
ATCACATGGG CACTTCCTCC TCAGCAGCAG CACTACAGGG TGAAGCCACT TCATTATATT 
TCCTGGCTTG TTGGACATGA AGGCAAAGGC AGCATTCTTT CTTATCTTAG AAAAAAGTGC 
TGGGCTCTTG CATTGTTTGG TGGAAATGGT GAGACAGGTT TTGAGCAAAA TTCTACTTAC 
TCAGTGTTCA GCATTTCTAT TACGCTGACT GATGAGGGCT ATGAGCATTT TTATGAGGTT 
GCCCACACTG TCTTTCAGTA TTTAAAAATG CTGCAGAAGC TAGGTCCAGA GAAAAGAGTT 
TTTGAAGAGA TTCAGAAAAT TGAGGATAAT GAATTTCATT ATCAAGAACA GACAGATCCA 
GTCGAGTATG TGGAGAACAT GTGTGAGAAC ATGCAGCTGT ACCCACGTCA GGACTTTCTC 
ACCGGAGATC AGCTTCTGTT TGAGTACAAG CCAGAAGTCA TTGCTGAGGC CCTTAATCAG 
CTAGTTCCTC AGAAGGCAAA TCTCGTTCTT CTCTCTGGTG CTAATGAGGG AAGATGTGAC 
CTCAAGGAGA AGTGGTTTGG GACTCAGTAT AGCATAGAAG ATATTGAAAA CTCTTGGACT 
GAACTATGGA AGAGTAATTT TGACTTAAAT CCAGACCTTC ATCTTCCAGC TGAAAATAAG 
TACATAGCCA CGGACTTTAC GTTGAAGGCT TTTGATTGCC CTGAAACTGA ATACCCTGCT 
AAAATTGTCA ATACCGCACA AGGCTGCTTG TGGTATAAGA AAGACAACAA ATTCAAAATC 
CCCAAAGCAT ATATACGATT TCATCTGATC TCACCTTTGA TACAGAAATC TGCAGCAAAT 
GTTGTCCTCT TTGACATCTT CGTGAATATT CTTACGCACA ACCTTGCCGA ACCCGCCTAT 
GAAGCTGATG TGGCACAGCT GGAGTATAAA CTGGTAGCTG GAGAACATGG GTTAATTATT 
CGAGTGAAAG GATTCAACCA CAAACTACCT CTGCTCTTTC AGCTCATTAT TGACTACCTG 
ACTGAGTTCA GTTCCACACC CGCTGTCTTT ACCATGATAA CTGAGCAACT GAAGAAGACC 
TACTTCAACA TCCTCATCAA GCCCGAGACT CTGGCCAAAG ATGTGCGGCT TTTAATTCTG 
GAATATTCCC GCTGGTCTAT GATTGACAAG TACCAAGCTT TGATGGATGG CCTTTCCCTT 
GATTCTCTGC TAAACTTTGT CAAGGATTTT AAATCCCAAC TTTTTGTTGA GGGCCTGGTC 
CAAGGGAATG TCACCAGCAC GGAATCGATG GATTTCCTAA AATATGTTGT TGACAAGCTG 
AACTTCGCGC CCCTGGAGCG GGAGATGCCT GTGCAGTTCC AGGTGGTGGA GCTGCCGAGC 
GGCCACCACC TGTGCAAAGT CAGAGCTCTG AATAAAGGGG ATGCCAACTC TGAAGTCACT 
GTGTACTACC AGATGCACAT GGAAGAGCCC TGCTTTGACT TCCTTCGCAC CAAGCAGACC 
CTTGGGTACC ATGTCTACCC AACCTGTAGG AACACATCTG GAATTCTAGG ATTTTCTGTA 
ACTGTTGGGA CTCAGGCAAC CAAATACAAC TCCGAAACTG TTGACAAGAA GATAGAGGAA 
TTTCTTTCCA GCTTTGAGGA GAAGATTGAG AACCTCACAG AAGATGCATT CAATACTCAG 
GTCACAGCAC TGATCAAGTT GAAAGAGTGT GAGGACACCC ACCTTGGGGA GGAGGTGGAC 
AGGAACTGGA ATGAAGTGGT GACACAGCAG TATCTCTTTG ACCGCCTTGC CCATGAGATT 
GAAGCCCTGA AGTCTTTCTC AAAGTCAGAC CTGGTCAGCT GGTTCAAGGC TCACAGAGGA 
CCTGGAAGTA AAATGCTCAG CGTTCATGTT GTTGGATATG GGAAGTATGA ACTGGAAGAG 
GATGGTGCCC CTTTTGGTGA GGATTCAAAT TCTCGTGAAG GGATGCAGCT AACCTACCTG 
CCACCCTCTC CTGTCCTGGC AGAATCCACC ACCCCCATTA CTGACATCAG GGCCTTCACA 
GCAACACTCA GCCTCTTCCC CTACCATAAA ATAGTCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide profiling of nardilysin target genes reveals its role in epigenetic regulation and cell cycle progression.
Scientific reports7(1)14801(2017 Nov)
Morita Y,Ohno M,Nishi K,Hiraoka Y,Saijo S,Matsuda S,Kita T,Kimura T,Nishi E

Nardilysin regulates inflammation, metaplasia, and tumors in murine stomach.
Scientific reports743052(2017 Feb)
Kimura Y,Ikuta K,Kimura T,Chiba T,Oshima H,Oshima M,Nishi E,Seno H

Nardilysin Is Required for Maintaining Pancreatic ?-Cell Function.
Diabetes65(10)3015-27(2016 10)
Nishi K,Sato Y,Ohno M,Hiraoka Y,Saijo S,Sakamoto J,Chen PM,Morita Y,Matsuda S,Iwasaki K,Sugizaki K,Harada N,Mukumoto Y,Kiyonari H,Furuyama K,Kawaguchi Y,Uemoto S,Kita T,Inagaki N,Kimura T,Nishi E

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Nrd1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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