Usp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp1
Entrez Gene ID	230484
Full Name	ubiquitin specific peptidase 1
General protein information	Preferred Names ubiquitin specific peptidase 1 Names ubiquitin carboxyl-terminal hydrolase 1 deubiquitinating enzyme 1 ubiquitin specific protease 1 ubiquitin thioesterase 1 ubiquitin thiolesterase 1 ubiquitin-specific-processing protease 1 usp1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 C6
Summary	This gene encodes a member of the ubiquitin-specific peptidase family. The encoded protein acts as a catalytic subunit in a heterodimeric deubiquitinating enzyme complex that deubiquitinates Fanconi anemia, complementation group D2, and plays a role in homologous recombination-mediated DNA repair. Disruption of this gene is associated with a Fanconi anemia-like phenotype and genomic instability. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 3, 12, and 15. [provided by RefSeq, Aug 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_146144.4	NP_666256.2	ubiquitin carboxyl-terminal hydrolase 1 isoform 1
XM_006502939.3	XP_006503002.1	ubiquitin carboxyl-terminal hydrolase 1 isoform X1
NM_001301414.1	NP_001288343.1	ubiquitin carboxyl-terminal hydrolase 1 isoform 1
NM_001356424.1	NP_001343353.1	ubiquitin carboxyl-terminal hydrolase 1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP1	NP_003359.3
	USP1	XP_001085998.2
Canis lupus familiaris (dog)	USP1	XP_005620277.1
Pan troglodytes (chimpanzee)	USP1	XP_003949480.1
Mus musculus (house mouse)	Usp1	NP_666256.2
Rattus norvegicus (Norway rat)	Usp1	NP_001015015.1
Bos taurus (cattle)	USP1	NP_001039689.1
Danio rerio (zebrafish)	usp1	NP_955873.1
Gallus gallus (chicken)	USP1	NP_001026461.1
Xenopus tropicalis (tropical clawed frog)	usp1	NP_001072581.1

Gene Ontology
Bibliography

Related articles in PubMed

The role of USP1 autocleavage in DNA interstrand crosslink repair.
Kim M, Kim JM
FEBS letters590(3)340-8(2016 Feb)

A novel role for the deubiquitinase USP1 in the control of centrosome duplication.
Jung JK, Jang SW, Kim JM
Cell cycle (Georgetown, Tex.)15(4)584-92(2016)

Inactivation of Uaf1 causes defective homologous recombination and early embryonic lethality in mice.
Park E, Kim JM, Primack B, Weinstock DM, Moreau LA, Parmar K, D'Andrea AD
Molecular and cellular biology33(22)4360-70(2013 Nov)

CAPNS1 regulates USP1 stability and maintenance of genome integrity.
Cataldo F, Peche LY, Klaric E, Brancolini C, Myers MP, Demarchi F, Schneider C
Molecular and cellular biology33(12)2485-96(2013 Jun)

FANCD2 activates transcription of TAp63 and suppresses tumorigenesis.
Park E, Kim H, Kim JM, Primack B, Vidal-Cardenas S, Xu Y, Price BD, Mills AA, D'Andrea AD
Molecular cell50(6)908-18(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest Usp1 down-regulation by autocleavage is critical for Usp1 to exert role in DNA interstrand crosslink repair; Usp1 role is de-ubiquitination of Fancd2 (Fanconi anemia complementation group D2) and Pcna (proliferating cell nuclear antigen).
Title: The role of USP1 autocleavage in DNA interstrand crosslink repair.

our results strongly suggest that Usp1 is involved in the regulation of centrosome duplication, at least in part via ID1, and Usp1 may exert its oncogenic activity, partially through inducing centrosome abnormality.
Title: A novel role for the deubiquitinase USP1 in the control of centrosome duplication.

Homozygous Uaf1(-/-) embryos died at embryonic day 7.5 (E7.5). These mutant embryos were small and developmentally retarded.
Title: Inactivation of Uaf1 causes defective homologous recombination and early embryonic lethality in mice.

Autocleaved Usp1 remains a deubiquitylase because its fragments remain associated with Uaf1, an enhancer of Usp1 activity, until the C-terminal fragment is selectively destroyed by the Arg/N-end rule pathway.
Title: The auto-generated fragment of the Usp1 deubiquitylase is a physiological substrate of the N-end rule pathway.

Bone marrow from Fancd2-/- mice and Usp1-/- mice exhibited marked hematopoietic defects.
Title: Hematopoietic stem cell defects in mice with deficiency of Fancd2 or Usp1.

The following Usp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18883	NM_001301414.1 Latest version!	Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu18883	NM_146144.4 Latest version!	Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu33477	NM_001356424.1 Latest version!	Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu33477	XM_006502939.3 Latest version!	Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp1 ( NM_146144.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666256.2 Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18883
Clone ID Related Accession (Same CDS sequence)	NM_146144.4 , NM_001301414.1
Accession Version	NM_146144.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK080882.1 and BG083613.1. On Aug 22, 2014 this sequence version replaced NM_146144.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK080882.1, BC018179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGCG TCATACCTAG TGAAAGTAAT GGGCTTTCAA GAGGCAGTCC ATCCAAGAAA 
AACAGACTGT CTCTGAAGTT TTTTCAGAAA AAGGAAACTA AAAGAGCCTT AGACTTTACT 
GATTCTCAAG AAAATGAAGA AAAAACTTCT GAATATAGAG GATCTGAGAT TGACCAAGTT 
GTTCCTGCTG CACAGTCCTC CCCAGTAAGC TGCGAGAAGA GAGAGAACTT GTTACCATTT 
GTGGGATTGA ATAACCTTGG CAACACTTGT TATCTGAATA GTATTCTTCA GGTGTTATAC 
TTTTGTCCTG GCTTTAAGAC TGGAGTGAAG CACTTATTTA ATATTATTTC AAGGAAGAAA 
GAAGCTCTGA AGGATGACTC TAATCAGAAG GATAAGGGAA GCTGCAAAGA AGAGTCTTTG 
GCAAGTTATG AGCTTATATG TAGTTTACAG TCCTTAATAA TTTCAGTTGA ACAGCTTCAG 
GCTAGTTTTC TCTTAAATCC AGAGAAATAT ACAGATGAAC TTGCTACACA GCCAAGGCGA 
CTGCTGAACA CACTCAGGGA ACTCAACCCT ATGTATGAAG GATTTCTACA GCATGATGCA 
CAGGAAGTGC TACAGTGTAT TCTGGGAAAC ATTCAAGAAA CATGCCAACT CCTAAAAAAA 
GAAGAAATAA AAAACCTGGC TGAATTATCC GGCAAGGTTG AAGAACAATC TCTTCAGAAA 
GAGGAAACAG GTGGAATTAC CAGCACAGAG ATTGACAGTA TGAGGAATAC GGAGGACGTC 
AAAGAGCAGC TCCCCAAAGG GAACTGGAAA AGAAAAAGTG ACAGTGAGTC GAGTAACGTG 
AAGAAAAAGG TTAAACTGTC CAGGGAATCC CAGCCATTGG AAGAAAACCA GAGACAAACC 
AGATCCAAGA GAAAAGCTAT CGGAGACACA CTAGAGGCTG CTCCTAAGAT CATCCCCAAG 
TGTGTTTCTG AAAGTGAGAG TGCAAAGCCC TCACAGAAGA AATCAAAAGT TAAAATAAAT 
TGGTTAAAGC CATCCACTAA GCAACCCAGC ATTCTTTCCA AGTTCTGTAG TCTTGGAAAA 
ATAACAACTA ACCAGCGCTC CAAAGGACAA CCTAAGGAAA AGGAGGGTGA TGTTGAAGAG 
GACTTGGAGA AGTACGGAAG TGACCACACA GCTAATGGTG GACCCGAATC TCCAGGAAGT 
AGCGTCACAC CTGTGGACAG CAGTGAAGCC AAGTCCGGAA ACAAAGGTGC TGAACAGATT 
GGCTTTGAGC TAGTAGAGAA ACTGTTTCAA GGTCAGCTCG TGTTAAGGAC TCGGTGTTTG 
GAATGTGAAA GCTTAACAGA AAGAAGAGAA GATTTCCAGG ATATTAGTGT TCCAGTGCAA 
GAAGATGAGC TTTCCAAGGT CGAGGAGAGC TCTGAAATTT CTCCAGAGCC AAAAACAGAA 
ATGAAGACCT TGAGATGGGC AATTTCACAG TTTGCTTCAG TGGAGAGAAT TGTAGGAGAG 
GATAAATACT TCTGTGAAAA TTGCCATCAT TATACGGAAG CAGAGCGAAG TCTCCTGTTT 
GATAAAATGC CTGAAGTTAT CACTATTCAT CTGAAGTGCT TTGCTGCTAG TGGCTTGGAG 
TTTGACTGTT ACGGTGGTGG ACTTTCCAAG ATCAACACTC CTTTATTGAC ACCTCTTAAA 
CTGTCACTAG AAGAATGGAG CACAAAGCCG ACGAATGACA GCTATGGATT ATTTGCCGTT 
GTGATGCACA GTGGCATTAC TATTAGTAGT GGGCACTATA CCGCATCTGT TAAAGTCACT 
GACCTTAACA GTCTAGAGCT CGATGAGGGG AATTTTGTGG TTGACCAGAT GTGTGAACTA 
GGTAAGCCAG AGCCACTGAC CGAGGAGCAA GCAAGGGGGA CGGCTGGGAA CTACGACGAT 
GAAGTATCAA TTAGAGTTGG TGGAAATGCC CAGCCAAGTA AAGTTTTGAA CAAAAAAAAT 
GTAGAAGGTA TTGGACTTCT CGGTGGACAA AAGAGCAAAG CAGATTATGA GCTGTACAAC 
AAAGCATCTA ATCCTGATAA GGTGGTTGGG ACACCATTCA CCGACAATAG AAATTCTGAA 
ACTAATGATA CTACTAATGG GACCCACGAA TCTGACAGGA ACAAGGAATC CAGTGACCAA 
ACAGGCGTTA ACATGAACGG ACTTGAGAAC AAAATTTCCT ATGTAGTGCA AAGCTTAAAG 
GAGTACGAGG GGAAGTGGTT GCTCTTTGAC GATTCTGAGG TAAAAGTGAC AGAAGAGAAA 
GACTTTCTGA ATTCTCTCTC CCCTTCTACA TCCCCTACGT CTACTCCCTA CTTGCTATTC 
TATAAAAAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666256.2
CDS	485..2839
Translation	MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKTSEYRGSEIDQVVPAAQSSPVSCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEEIKNLAELSGKVEEQSLQKEETGGITSTEIDSMRNTEDVKEQLPKGNWKRKSDSESSNVKKKVKLSRESQPLEENQRQTRSKRKAIGDTLEAAPKIIPKCVSESESAKPSQKKSKVKINWLKPSTKQPSILSKFCSLGKITTNQRSKGQPKEKEGDVEEDLEKYGSDHTANGGPESPGSSVTPVDSSEAKSGNKGAEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDEGNFVVDQMCELGKPEPLTEEQARGTAGNYDDEVSIRVGGNAQPSKVLNKKNVEGIGLLGGQKSKADYELYNKASNPDKVVGTPFTDNRNSETNDTTNGTHESDRNKESSDQTGVNMNGLENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL

Target ORF information:

RefSeq Version	NM_146144.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146144.4

ORF Insert Sequence:

ATGCCTGGCG TCATACCTAG TGAAAGTAAT GGGCTTTCAA GAGGCAGTCC ATCCAAGAAA 
AACAGACTGT CTCTGAAGTT TTTTCAGAAA AAGGAAACTA AAAGAGCCTT AGACTTTACT 
GATTCTCAAG AAAATGAAGA AAAAACTTCT GAATATAGAG GATCTGAGAT TGACCAAGTT 
GTTCCTGCTG CACAGTCCTC CCCAGTAAGC TGCGAGAAGA GAGAGAACTT GTTACCATTT 
GTGGGATTGA ATAACCTTGG CAACACTTGT TATCTGAATA GTATTCTTCA GGTGTTATAC 
TTTTGTCCTG GCTTTAAGAC TGGAGTGAAG CACTTATTTA ATATTATTTC AAGGAAGAAA 
GAAGCTCTGA AGGATGACTC TAATCAGAAG GATAAGGGAA GCTGCAAAGA AGAGTCTTTG 
GCAAGTTATG AGCTTATATG TAGTTTACAG TCCTTAATAA TTTCAGTTGA ACAGCTTCAG 
GCTAGTTTTC TCTTAAATCC AGAGAAATAT ACAGATGAAC TTGCTACACA GCCAAGGCGA 
CTGCTGAACA CACTCAGGGA ACTCAACCCT ATGTATGAAG GATTTCTACA GCATGATGCA 
CAGGAAGTGC TACAGTGTAT TCTGGGAAAC ATTCAAGAAA CATGCCAACT CCTAAAAAAA 
GAAGAAATAA AAAACCTGGC TGAATTATCC GGCAAGGTTG AAGAACAATC TCTTCAGAAA 
GAGGAAACAG GTGGAATTAC CAGCACAGAG ATTGACAGTA TGAGGAATAC GGAGGACGTC 
AAAGAGCAGC TCCCCAAAGG GAACTGGAAA AGAAAAAGTG ACAGTGAGTC GAGTAACGTG 
AAGAAAAAGG TTAAACTGTC CAGGGAATCC CAGCCATTGG AAGAAAACCA GAGACAAACC 
AGATCCAAGA GAAAAGCTAT CGGAGACACA CTAGAGGCTG CTCCTAAGAT CATCCCCAAG 
TGTGTTTCTG AAAGTGAGAG TGCAAAGCCC TCACAGAAGA AATCAAAAGT TAAAATAAAT 
TGGTTAAAGC CATCCACTAA GCAACCCAGC ATTCTTTCCA AGTTCTGTAG TCTTGGAAAA 
ATAACAACTA ACCAGCGCTC CAAAGGACAA CCTAAGGAAA AGGAGGGTGA TGTTGAAGAG 
GACTTGGAGA AGTACGGAAG TGACCACACA GCTAATGGTG GACCCGAATC TCCAGGAAGT 
AGCGTCACAC CTGTGGACAG CAGTGAAGCC AAGTCCGGAA ACAAAGGTGC TGAACAGATT 
GGCTTTGAGC TAGTAGAGAA ACTGTTTCAA GGTCAGCTCG TGTTAAGGAC TCGGTGTTTG 
GAATGTGAAA GCTTAACAGA AAGAAGAGAA GATTTCCAGG ATATTAGTGT TCCAGTGCAA 
GAAGATGAGC TTTCCAAGGT CGAGGAGAGC TCTGAAATTT CTCCAGAGCC AAAAACAGAA 
ATGAAGACCT TGAGATGGGC AATTTCACAG TTTGCTTCAG TGGAGAGAAT TGTAGGAGAG 
GATAAATACT TCTGTGAAAA TTGCCATCAT TATACGGAAG CAGAGCGAAG TCTCCTGTTT 
GATAAAATGC CTGAAGTTAT CACTATTCAT CTGAAGTGCT TTGCTGCTAG TGGCTTGGAG 
TTTGACTGTT ACGGTGGTGG ACTTTCCAAG ATCAACACTC CTTTATTGAC ACCTCTTAAA 
CTGTCACTAG AAGAATGGAG CACAAAGCCG ACGAATGACA GCTATGGATT ATTTGCCGTT 
GTGATGCACA GTGGCATTAC TATTAGTAGT GGGCACTATA CCGCATCTGT TAAAGTCACT 
GACCTTAACA GTCTAGAGCT CGATGAGGGG AATTTTGTGG TTGACCAGAT GTGTGAACTA 
GGTAAGCCAG AGCCACTGAC CGAGGAGCAA GCAAGGGGGA CGGCTGGGAA CTACGACGAT 
GAAGTATCAA TTAGAGTTGG TGGAAATGCC CAGCCAAGTA AAGTTTTGAA CAAAAAAAAT 
GTAGAAGGTA TTGGACTTCT CGGTGGACAA AAGAGCAAAG CAGATTATGA GCTGTACAAC 
AAAGCATCTA ATCCTGATAA GGTGGTTGGG ACACCATTCA CCGACAATAG AAATTCTGAA 
ACTAATGATA CTACTAATGG GACCCACGAA TCTGACAGGA ACAAGGAATC CAGTGACCAA 
ACAGGCGTTA ACATGAACGG ACTTGAGAAC AAAATTTCCT ATGTAGTGCA AAGCTTAAAG 
GAGTACGAGG GGAAGTGGTT GCTCTTTGAC GATTCTGAGG TAAAAGTGAC AGAAGAGAAA 
GACTTTCTGA ATTCTCTCTC CCCTTCTACA TCCCCTACGT CTACTCCCTA CTTGCTATTC 
TATAAAAAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp1 ( NM_001301414.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288343.1 Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18883
Clone ID Related Accession (Same CDS sequence)	NM_146144.4 , NM_001301414.1
Accession Version	NM_001301414.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK080882.1, BY713495.1 and BG083613.1. On Aug 22, 2014 this sequence version replaced XM_006502938.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC020007.1, SRR1660811.244822.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGCG TCATACCTAG TGAAAGTAAT GGGCTTTCAA GAGGCAGTCC ATCCAAGAAA 
AACAGACTGT CTCTGAAGTT TTTTCAGAAA AAGGAAACTA AAAGAGCCTT AGACTTTACT 
GATTCTCAAG AAAATGAAGA AAAAACTTCT GAATATAGAG GATCTGAGAT TGACCAAGTT 
GTTCCTGCTG CACAGTCCTC CCCAGTAAGC TGCGAGAAGA GAGAGAACTT GTTACCATTT 
GTGGGATTGA ATAACCTTGG CAACACTTGT TATCTGAATA GTATTCTTCA GGTGTTATAC 
TTTTGTCCTG GCTTTAAGAC TGGAGTGAAG CACTTATTTA ATATTATTTC AAGGAAGAAA 
GAAGCTCTGA AGGATGACTC TAATCAGAAG GATAAGGGAA GCTGCAAAGA AGAGTCTTTG 
GCAAGTTATG AGCTTATATG TAGTTTACAG TCCTTAATAA TTTCAGTTGA ACAGCTTCAG 
GCTAGTTTTC TCTTAAATCC AGAGAAATAT ACAGATGAAC TTGCTACACA GCCAAGGCGA 
CTGCTGAACA CACTCAGGGA ACTCAACCCT ATGTATGAAG GATTTCTACA GCATGATGCA 
CAGGAAGTGC TACAGTGTAT TCTGGGAAAC ATTCAAGAAA CATGCCAACT CCTAAAAAAA 
GAAGAAATAA AAAACCTGGC TGAATTATCC GGCAAGGTTG AAGAACAATC TCTTCAGAAA 
GAGGAAACAG GTGGAATTAC CAGCACAGAG ATTGACAGTA TGAGGAATAC GGAGGACGTC 
AAAGAGCAGC TCCCCAAAGG GAACTGGAAA AGAAAAAGTG ACAGTGAGTC GAGTAACGTG 
AAGAAAAAGG TTAAACTGTC CAGGGAATCC CAGCCATTGG AAGAAAACCA GAGACAAACC 
AGATCCAAGA GAAAAGCTAT CGGAGACACA CTAGAGGCTG CTCCTAAGAT CATCCCCAAG 
TGTGTTTCTG AAAGTGAGAG TGCAAAGCCC TCACAGAAGA AATCAAAAGT TAAAATAAAT 
TGGTTAAAGC CATCCACTAA GCAACCCAGC ATTCTTTCCA AGTTCTGTAG TCTTGGAAAA 
ATAACAACTA ACCAGCGCTC CAAAGGACAA CCTAAGGAAA AGGAGGGTGA TGTTGAAGAG 
GACTTGGAGA AGTACGGAAG TGACCACACA GCTAATGGTG GACCCGAATC TCCAGGAAGT 
AGCGTCACAC CTGTGGACAG CAGTGAAGCC AAGTCCGGAA ACAAAGGTGC TGAACAGATT 
GGCTTTGAGC TAGTAGAGAA ACTGTTTCAA GGTCAGCTCG TGTTAAGGAC TCGGTGTTTG 
GAATGTGAAA GCTTAACAGA AAGAAGAGAA GATTTCCAGG ATATTAGTGT TCCAGTGCAA 
GAAGATGAGC TTTCCAAGGT CGAGGAGAGC TCTGAAATTT CTCCAGAGCC AAAAACAGAA 
ATGAAGACCT TGAGATGGGC AATTTCACAG TTTGCTTCAG TGGAGAGAAT TGTAGGAGAG 
GATAAATACT TCTGTGAAAA TTGCCATCAT TATACGGAAG CAGAGCGAAG TCTCCTGTTT 
GATAAAATGC CTGAAGTTAT CACTATTCAT CTGAAGTGCT TTGCTGCTAG TGGCTTGGAG 
TTTGACTGTT ACGGTGGTGG ACTTTCCAAG ATCAACACTC CTTTATTGAC ACCTCTTAAA 
CTGTCACTAG AAGAATGGAG CACAAAGCCG ACGAATGACA GCTATGGATT ATTTGCCGTT 
GTGATGCACA GTGGCATTAC TATTAGTAGT GGGCACTATA CCGCATCTGT TAAAGTCACT 
GACCTTAACA GTCTAGAGCT CGATGAGGGG AATTTTGTGG TTGACCAGAT GTGTGAACTA 
GGTAAGCCAG AGCCACTGAC CGAGGAGCAA GCAAGGGGGA CGGCTGGGAA CTACGACGAT 
GAAGTATCAA TTAGAGTTGG TGGAAATGCC CAGCCAAGTA AAGTTTTGAA CAAAAAAAAT 
GTAGAAGGTA TTGGACTTCT CGGTGGACAA AAGAGCAAAG CAGATTATGA GCTGTACAAC 
AAAGCATCTA ATCCTGATAA GGTGGTTGGG ACACCATTCA CCGACAATAG AAATTCTGAA 
ACTAATGATA CTACTAATGG GACCCACGAA TCTGACAGGA ACAAGGAATC CAGTGACCAA 
ACAGGCGTTA ACATGAACGG ACTTGAGAAC AAAATTTCCT ATGTAGTGCA AAGCTTAAAG 
GAGTACGAGG GGAAGTGGTT GCTCTTTGAC GATTCTGAGG TAAAAGTGAC AGAAGAGAAA 
GACTTTCTGA ATTCTCTCTC CCCTTCTACA TCCCCTACGT CTACTCCCTA CTTGCTATTC 
TATAAAAAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288343.1
CDS	504..2858
Translation	MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKTSEYRGSEIDQVVPAAQSSPVSCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEEIKNLAELSGKVEEQSLQKEETGGITSTEIDSMRNTEDVKEQLPKGNWKRKSDSESSNVKKKVKLSRESQPLEENQRQTRSKRKAIGDTLEAAPKIIPKCVSESESAKPSQKKSKVKINWLKPSTKQPSILSKFCSLGKITTNQRSKGQPKEKEGDVEEDLEKYGSDHTANGGPESPGSSVTPVDSSEAKSGNKGAEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDEGNFVVDQMCELGKPEPLTEEQARGTAGNYDDEVSIRVGGNAQPSKVLNKKNVEGIGLLGGQKSKADYELYNKASNPDKVVGTPFTDNRNSETNDTTNGTHESDRNKESSDQTGVNMNGLENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL

Target ORF information:

RefSeq Version	NM_001301414.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301414.1

ORF Insert Sequence:

ATGCCTGGCG TCATACCTAG TGAAAGTAAT GGGCTTTCAA GAGGCAGTCC ATCCAAGAAA 
AACAGACTGT CTCTGAAGTT TTTTCAGAAA AAGGAAACTA AAAGAGCCTT AGACTTTACT 
GATTCTCAAG AAAATGAAGA AAAAACTTCT GAATATAGAG GATCTGAGAT TGACCAAGTT 
GTTCCTGCTG CACAGTCCTC CCCAGTAAGC TGCGAGAAGA GAGAGAACTT GTTACCATTT 
GTGGGATTGA ATAACCTTGG CAACACTTGT TATCTGAATA GTATTCTTCA GGTGTTATAC 
TTTTGTCCTG GCTTTAAGAC TGGAGTGAAG CACTTATTTA ATATTATTTC AAGGAAGAAA 
GAAGCTCTGA AGGATGACTC TAATCAGAAG GATAAGGGAA GCTGCAAAGA AGAGTCTTTG 
GCAAGTTATG AGCTTATATG TAGTTTACAG TCCTTAATAA TTTCAGTTGA ACAGCTTCAG 
GCTAGTTTTC TCTTAAATCC AGAGAAATAT ACAGATGAAC TTGCTACACA GCCAAGGCGA 
CTGCTGAACA CACTCAGGGA ACTCAACCCT ATGTATGAAG GATTTCTACA GCATGATGCA 
CAGGAAGTGC TACAGTGTAT TCTGGGAAAC ATTCAAGAAA CATGCCAACT CCTAAAAAAA 
GAAGAAATAA AAAACCTGGC TGAATTATCC GGCAAGGTTG AAGAACAATC TCTTCAGAAA 
GAGGAAACAG GTGGAATTAC CAGCACAGAG ATTGACAGTA TGAGGAATAC GGAGGACGTC 
AAAGAGCAGC TCCCCAAAGG GAACTGGAAA AGAAAAAGTG ACAGTGAGTC GAGTAACGTG 
AAGAAAAAGG TTAAACTGTC CAGGGAATCC CAGCCATTGG AAGAAAACCA GAGACAAACC 
AGATCCAAGA GAAAAGCTAT CGGAGACACA CTAGAGGCTG CTCCTAAGAT CATCCCCAAG 
TGTGTTTCTG AAAGTGAGAG TGCAAAGCCC TCACAGAAGA AATCAAAAGT TAAAATAAAT 
TGGTTAAAGC CATCCACTAA GCAACCCAGC ATTCTTTCCA AGTTCTGTAG TCTTGGAAAA 
ATAACAACTA ACCAGCGCTC CAAAGGACAA CCTAAGGAAA AGGAGGGTGA TGTTGAAGAG 
GACTTGGAGA AGTACGGAAG TGACCACACA GCTAATGGTG GACCCGAATC TCCAGGAAGT 
AGCGTCACAC CTGTGGACAG CAGTGAAGCC AAGTCCGGAA ACAAAGGTGC TGAACAGATT 
GGCTTTGAGC TAGTAGAGAA ACTGTTTCAA GGTCAGCTCG TGTTAAGGAC TCGGTGTTTG 
GAATGTGAAA GCTTAACAGA AAGAAGAGAA GATTTCCAGG ATATTAGTGT TCCAGTGCAA 
GAAGATGAGC TTTCCAAGGT CGAGGAGAGC TCTGAAATTT CTCCAGAGCC AAAAACAGAA 
ATGAAGACCT TGAGATGGGC AATTTCACAG TTTGCTTCAG TGGAGAGAAT TGTAGGAGAG 
GATAAATACT TCTGTGAAAA TTGCCATCAT TATACGGAAG CAGAGCGAAG TCTCCTGTTT 
GATAAAATGC CTGAAGTTAT CACTATTCAT CTGAAGTGCT TTGCTGCTAG TGGCTTGGAG 
TTTGACTGTT ACGGTGGTGG ACTTTCCAAG ATCAACACTC CTTTATTGAC ACCTCTTAAA 
CTGTCACTAG AAGAATGGAG CACAAAGCCG ACGAATGACA GCTATGGATT ATTTGCCGTT 
GTGATGCACA GTGGCATTAC TATTAGTAGT GGGCACTATA CCGCATCTGT TAAAGTCACT 
GACCTTAACA GTCTAGAGCT CGATGAGGGG AATTTTGTGG TTGACCAGAT GTGTGAACTA 
GGTAAGCCAG AGCCACTGAC CGAGGAGCAA GCAAGGGGGA CGGCTGGGAA CTACGACGAT 
GAAGTATCAA TTAGAGTTGG TGGAAATGCC CAGCCAAGTA AAGTTTTGAA CAAAAAAAAT 
GTAGAAGGTA TTGGACTTCT CGGTGGACAA AAGAGCAAAG CAGATTATGA GCTGTACAAC 
AAAGCATCTA ATCCTGATAA GGTGGTTGGG ACACCATTCA CCGACAATAG AAATTCTGAA 
ACTAATGATA CTACTAATGG GACCCACGAA TCTGACAGGA ACAAGGAATC CAGTGACCAA 
ACAGGCGTTA ACATGAACGG ACTTGAGAAC AAAATTTCCT ATGTAGTGCA AAGCTTAAAG 
GAGTACGAGG GGAAGTGGTT GCTCTTTGAC GATTCTGAGG TAAAAGTGAC AGAAGAGAAA 
GACTTTCTGA ATTCTCTCTC CCCTTCTACA TCCCCTACGT CTACTCCCTA CTTGCTATTC 
TATAAAAAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp1 ( NM_001356424.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343353.1 Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant 3, mRNA.

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CloneID	OMu33477
Clone ID Related Accession (Same CDS sequence)	XM_006502939.3 , NM_001356424.1
Accession Version	NM_001356424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK080882.1 and BG083613.1. On Oct 4, 2017 this sequence version replaced XM_006502939.3. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.69611.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGGTG ACCAAGTTGT TCCTGCTGCA CAGTCCTCCC CAGTAAGCTG CGAGAAGAGA 
GAGAACTTGT TACCATTTGT GGGATTGAAT AACCTTGGCA ACACTTGTTA TCTGAATAGT 
ATTCTTCAGG TGTTATACTT TTGTCCTGGC TTTAAGACTG GAGTGAAGCA CTTATTTAAT 
ATTATTTCAA GGAAGAAAGA AGCTCTGAAG GATGACTCTA ATCAGAAGGA TAAGGGAAGC 
TGCAAAGAAG AGTCTTTGGC AAGTTATGAG CTTATATGTA GTTTACAGTC CTTAATAATT 
TCAGTTGAAC AGCTTCAGGC TAGTTTTCTC TTAAATCCAG AGAAATATAC AGATGAACTT 
GCTACACAGC CAAGGCGACT GCTGAACACA CTCAGGGAAC TCAACCCTAT GTATGAAGGA 
TTTCTACAGC ATGATGCACA GGAAGTGCTA CAGTGTATTC TGGGAAACAT TCAAGAAACA 
TGCCAACTCC TAAAAAAAGA AGAAATAAAA AACCTGGCTG AATTATCCGG CAAGGTTGAA 
GAACAATCTC TTCAGAAAGA GGAAACAGGT GGAATTACCA GCACAGAGAT TGACAGTATG 
AGGAATACGG AGGACGTCAA AGAGCAGCTC CCCAAAGGGA ACTGGAAAAG AAAAAGTGAC 
AGTGAGTCGA GTAACGTGAA GAAAAAGGTT AAACTGTCCA GGGAATCCCA GCCATTGGAA 
GAAAACCAGA GACAAACCAG ATCCAAGAGA AAAGCTATCG GAGACACACT AGAGGCTGCT 
CCTAAGATCA TCCCCAAGTG TGTTTCTGAA AGTGAGAGTG CAAAGCCCTC ACAGAAGAAA 
TCAAAAGTTA AAATAAATTG GTTAAAGCCA TCCACTAAGC AACCCAGCAT TCTTTCCAAG 
TTCTGTAGTC TTGGAAAAAT AACAACTAAC CAGCGCTCCA AAGGACAACC TAAGGAAAAG 
GAGGGTGATG TTGAAGAGGA CTTGGAGAAG TACGGAAGTG ACCACACAGC TAATGGTGGA 
CCCGAATCTC CAGGAAGTAG CGTCACACCT GTGGACAGCA GTGAAGCCAA GTCCGGAAAC 
AAAGGTGCTG AACAGATTGG CTTTGAGCTA GTAGAGAAAC TGTTTCAAGG TCAGCTCGTG 
TTAAGGACTC GGTGTTTGGA ATGTGAAAGC TTAACAGAAA GAAGAGAAGA TTTCCAGGAT 
ATTAGTGTTC CAGTGCAAGA AGATGAGCTT TCCAAGGTCG AGGAGAGCTC TGAAATTTCT 
CCAGAGCCAA AAACAGAAAT GAAGACCTTG AGATGGGCAA TTTCACAGTT TGCTTCAGTG 
GAGAGAATTG TAGGAGAGGA TAAATACTTC TGTGAAAATT GCCATCATTA TACGGAAGCA 
GAGCGAAGTC TCCTGTTTGA TAAAATGCCT GAAGTTATCA CTATTCATCT GAAGTGCTTT 
GCTGCTAGTG GCTTGGAGTT TGACTGTTAC GGTGGTGGAC TTTCCAAGAT CAACACTCCT 
TTATTGACAC CTCTTAAACT GTCACTAGAA GAATGGAGCA CAAAGCCGAC GAATGACAGC 
TATGGATTAT TTGCCGTTGT GATGCACAGT GGCATTACTA TTAGTAGTGG GCACTATACC 
GCATCTGTTA AAGTCACTGA CCTTAACAGT CTAGAGCTCG ATGAGGGGAA TTTTGTGGTT 
GACCAGATGT GTGAACTAGG TAAGCCAGAG CCACTGACCG AGGAGCAAGC AAGGGGGACG 
GCTGGGAACT ACGACGATGA AGTATCAATT AGAGTTGGTG GAAATGCCCA GCCAAGTAAA 
GTTTTGAACA AAAAAAATGT AGAAGGTATT GGACTTCTCG GTGGACAAAA GAGCAAAGCA 
GATTATGAGC TGTACAACAA AGCATCTAAT CCTGATAAGG TGGTTGGGAC ACCATTCACC 
GACAATAGAA ATTCTGAAAC TAATGATACT ACTAATGGGA CCCACGAATC TGACAGGAAC 
AAGGAATCCA GTGACCAAAC AGGCGTTAAC ATGAACGGAC TTGAGAACAA AATTTCCTAT 
GTAGTGCAAA GCTTAAAGGA GTACGAGGGG AAGTGGTTGC TCTTTGACGA TTCTGAGGTA 
AAAGTGACAG AAGAGAAAGA CTTTCTGAAT TCTCTCTCCC CTTCTACATC CCCTACGTCT 
ACTCCCTACT TGCTATTCTA TAAAAAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343353.1
CDS	427..2619
Translation	MTGDQVVPAAQSSPVSCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEEIKNLAELSGKVEEQSLQKEETGGITSTEIDSMRNTEDVKEQLPKGNWKRKSDSESSNVKKKVKLSRESQPLEENQRQTRSKRKAIGDTLEAAPKIIPKCVSESESAKPSQKKSKVKINWLKPSTKQPSILSKFCSLGKITTNQRSKGQPKEKEGDVEEDLEKYGSDHTANGGPESPGSSVTPVDSSEAKSGNKGAEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDEGNFVVDQMCELGKPEPLTEEQARGTAGNYDDEVSIRVGGNAQPSKVLNKKNVEGIGLLGGQKSKADYELYNKASNPDKVVGTPFTDNRNSETNDTTNGTHESDRNKESSDQTGVNMNGLENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL

Target ORF information:

RefSeq Version	NM_001356424.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356424.1

ORF Insert Sequence:

ATGACCGGTG ACCAAGTTGT TCCTGCTGCA CAGTCCTCCC CAGTAAGCTG CGAGAAGAGA 
GAGAACTTGT TACCATTTGT GGGATTGAAT AACCTTGGCA ACACTTGTTA TCTGAATAGT 
ATTCTTCAGG TGTTATACTT TTGTCCTGGC TTTAAGACTG GAGTGAAGCA CTTATTTAAT 
ATTATTTCAA GGAAGAAAGA AGCTCTGAAG GATGACTCTA ATCAGAAGGA TAAGGGAAGC 
TGCAAAGAAG AGTCTTTGGC AAGTTATGAG CTTATATGTA GTTTACAGTC CTTAATAATT 
TCAGTTGAAC AGCTTCAGGC TAGTTTTCTC TTAAATCCAG AGAAATATAC AGATGAACTT 
GCTACACAGC CAAGGCGACT GCTGAACACA CTCAGGGAAC TCAACCCTAT GTATGAAGGA 
TTTCTACAGC ATGATGCACA GGAAGTGCTA CAGTGTATTC TGGGAAACAT TCAAGAAACA 
TGCCAACTCC TAAAAAAAGA AGAAATAAAA AACCTGGCTG AATTATCCGG CAAGGTTGAA 
GAACAATCTC TTCAGAAAGA GGAAACAGGT GGAATTACCA GCACAGAGAT TGACAGTATG 
AGGAATACGG AGGACGTCAA AGAGCAGCTC CCCAAAGGGA ACTGGAAAAG AAAAAGTGAC 
AGTGAGTCGA GTAACGTGAA GAAAAAGGTT AAACTGTCCA GGGAATCCCA GCCATTGGAA 
GAAAACCAGA GACAAACCAG ATCCAAGAGA AAAGCTATCG GAGACACACT AGAGGCTGCT 
CCTAAGATCA TCCCCAAGTG TGTTTCTGAA AGTGAGAGTG CAAAGCCCTC ACAGAAGAAA 
TCAAAAGTTA AAATAAATTG GTTAAAGCCA TCCACTAAGC AACCCAGCAT TCTTTCCAAG 
TTCTGTAGTC TTGGAAAAAT AACAACTAAC CAGCGCTCCA AAGGACAACC TAAGGAAAAG 
GAGGGTGATG TTGAAGAGGA CTTGGAGAAG TACGGAAGTG ACCACACAGC TAATGGTGGA 
CCCGAATCTC CAGGAAGTAG CGTCACACCT GTGGACAGCA GTGAAGCCAA GTCCGGAAAC 
AAAGGTGCTG AACAGATTGG CTTTGAGCTA GTAGAGAAAC TGTTTCAAGG TCAGCTCGTG 
TTAAGGACTC GGTGTTTGGA ATGTGAAAGC TTAACAGAAA GAAGAGAAGA TTTCCAGGAT 
ATTAGTGTTC CAGTGCAAGA AGATGAGCTT TCCAAGGTCG AGGAGAGCTC TGAAATTTCT 
CCAGAGCCAA AAACAGAAAT GAAGACCTTG AGATGGGCAA TTTCACAGTT TGCTTCAGTG 
GAGAGAATTG TAGGAGAGGA TAAATACTTC TGTGAAAATT GCCATCATTA TACGGAAGCA 
GAGCGAAGTC TCCTGTTTGA TAAAATGCCT GAAGTTATCA CTATTCATCT GAAGTGCTTT 
GCTGCTAGTG GCTTGGAGTT TGACTGTTAC GGTGGTGGAC TTTCCAAGAT CAACACTCCT 
TTATTGACAC CTCTTAAACT GTCACTAGAA GAATGGAGCA CAAAGCCGAC GAATGACAGC 
TATGGATTAT TTGCCGTTGT GATGCACAGT GGCATTACTA TTAGTAGTGG GCACTATACC 
GCATCTGTTA AAGTCACTGA CCTTAACAGT CTAGAGCTCG ATGAGGGGAA TTTTGTGGTT 
GACCAGATGT GTGAACTAGG TAAGCCAGAG CCACTGACCG AGGAGCAAGC AAGGGGGACG 
GCTGGGAACT ACGACGATGA AGTATCAATT AGAGTTGGTG GAAATGCCCA GCCAAGTAAA 
GTTTTGAACA AAAAAAATGT AGAAGGTATT GGACTTCTCG GTGGACAAAA GAGCAAAGCA 
GATTATGAGC TGTACAACAA AGCATCTAAT CCTGATAAGG TGGTTGGGAC ACCATTCACC 
GACAATAGAA ATTCTGAAAC TAATGATACT ACTAATGGGA CCCACGAATC TGACAGGAAC 
AAGGAATCCA GTGACCAAAC AGGCGTTAAC ATGAACGGAC TTGAGAACAA AATTTCCTAT 
GTAGTGCAAA GCTTAAAGGA GTACGAGGGG AAGTGGTTGC TCTTTGACGA TTCTGAGGTA 
AAAGTGACAG AAGAGAAAGA CTTTCTGAAT TCTCTCTCCC CTTCTACATC CCCTACGTCT 
ACTCCCTACT TGCTATTCTA TAAAAAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp1 ( XM_006502939.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503002.1 Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant X1, mRNA.

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CloneID	OMu33477
Clone ID Related Accession (Same CDS sequence)	XM_006502939.3 , NM_001356424.1
Accession Version	XM_006502939.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	ubiquitin carboxyl-terminal hydrolase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502939.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGGTG ACCAAGTTGT TCCTGCTGCA CAGTCCTCCC CAGTAAGCTG CGAGAAGAGA 
GAGAACTTGT TACCATTTGT GGGATTGAAT AACCTTGGCA ACACTTGTTA TCTGAATAGT 
ATTCTTCAGG TGTTATACTT TTGTCCTGGC TTTAAGACTG GAGTGAAGCA CTTATTTAAT 
ATTATTTCAA GGAAGAAAGA AGCTCTGAAG GATGACTCTA ATCAGAAGGA TAAGGGAAGC 
TGCAAAGAAG AGTCTTTGGC AAGTTATGAG CTTATATGTA GTTTACAGTC CTTAATAATT 
TCAGTTGAAC AGCTTCAGGC TAGTTTTCTC TTAAATCCAG AGAAATATAC AGATGAACTT 
GCTACACAGC CAAGGCGACT GCTGAACACA CTCAGGGAAC TCAACCCTAT GTATGAAGGA 
TTTCTACAGC ATGATGCACA GGAAGTGCTA CAGTGTATTC TGGGAAACAT TCAAGAAACA 
TGCCAACTCC TAAAAAAAGA AGAAATAAAA AACCTGGCTG AATTATCCGG CAAGGTTGAA 
GAACAATCTC TTCAGAAAGA GGAAACAGGT GGAATTACCA GCACAGAGAT TGACAGTATG 
AGGAATACGG AGGACGTCAA AGAGCAGCTC CCCAAAGGGA ACTGGAAAAG AAAAAGTGAC 
AGTGAGTCGA GTAACGTGAA GAAAAAGGTT AAACTGTCCA GGGAATCCCA GCCATTGGAA 
GAAAACCAGA GACAAACCAG ATCCAAGAGA AAAGCTATCG GAGACACACT AGAGGCTGCT 
CCTAAGATCA TCCCCAAGTG TGTTTCTGAA AGTGAGAGTG CAAAGCCCTC ACAGAAGAAA 
TCAAAAGTTA AAATAAATTG GTTAAAGCCA TCCACTAAGC AACCCAGCAT TCTTTCCAAG 
TTCTGTAGTC TTGGAAAAAT AACAACTAAC CAGCGCTCCA AAGGACAACC TAAGGAAAAG 
GAGGGTGATG TTGAAGAGGA CTTGGAGAAG TACGGAAGTG ACCACACAGC TAATGGTGGA 
CCCGAATCTC CAGGAAGTAG CGTCACACCT GTGGACAGCA GTGAAGCCAA GTCCGGAAAC 
AAAGGTGCTG AACAGATTGG CTTTGAGCTA GTAGAGAAAC TGTTTCAAGG TCAGCTCGTG 
TTAAGGACTC GGTGTTTGGA ATGTGAAAGC TTAACAGAAA GAAGAGAAGA TTTCCAGGAT 
ATTAGTGTTC CAGTGCAAGA AGATGAGCTT TCCAAGGTCG AGGAGAGCTC TGAAATTTCT 
CCAGAGCCAA AAACAGAAAT GAAGACCTTG AGATGGGCAA TTTCACAGTT TGCTTCAGTG 
GAGAGAATTG TAGGAGAGGA TAAATACTTC TGTGAAAATT GCCATCATTA TACGGAAGCA 
GAGCGAAGTC TCCTGTTTGA TAAAATGCCT GAAGTTATCA CTATTCATCT GAAGTGCTTT 
GCTGCTAGTG GCTTGGAGTT TGACTGTTAC GGTGGTGGAC TTTCCAAGAT CAACACTCCT 
TTATTGACAC CTCTTAAACT GTCACTAGAA GAATGGAGCA CAAAGCCGAC GAATGACAGC 
TATGGATTAT TTGCCGTTGT GATGCACAGT GGCATTACTA TTAGTAGTGG GCACTATACC 
GCATCTGTTA AAGTCACTGA CCTTAACAGT CTAGAGCTCG ATGAGGGGAA TTTTGTGGTT 
GACCAGATGT GTGAACTAGG TAAGCCAGAG CCACTGACCG AGGAGCAAGC AAGGGGGACG 
GCTGGGAACT ACGACGATGA AGTATCAATT AGAGTTGGTG GAAATGCCCA GCCAAGTAAA 
GTTTTGAACA AAAAAAATGT AGAAGGTATT GGACTTCTCG GTGGACAAAA GAGCAAAGCA 
GATTATGAGC TGTACAACAA AGCATCTAAT CCTGATAAGG TGGTTGGGAC ACCATTCACC 
GACAATAGAA ATTCTGAAAC TAATGATACT ACTAATGGGA CCCACGAATC TGACAGGAAC 
AAGGAATCCA GTGACCAAAC AGGCGTTAAC ATGAACGGAC TTGAGAACAA AATTTCCTAT 
GTAGTGCAAA GCTTAAAGGA GTACGAGGGG AAGTGGTTGC TCTTTGACGA TTCTGAGGTA 
AAAGTGACAG AAGAGAAAGA CTTTCTGAAT TCTCTCTCCC CTTCTACATC CCCTACGTCT 
ACTCCCTACT TGCTATTCTA TAAAAAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503002.1
CDS	614..2806
Translation	MTGDQVVPAAQSSPVSCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGFLQHDAQEVLQCILGNIQETCQLLKKEEIKNLAELSGKVEEQSLQKEETGGITSTEIDSMRNTEDVKEQLPKGNWKRKSDSESSNVKKKVKLSRESQPLEENQRQTRSKRKAIGDTLEAAPKIIPKCVSESESAKPSQKKSKVKINWLKPSTKQPSILSKFCSLGKITTNQRSKGQPKEKEGDVEEDLEKYGSDHTANGGPESPGSSVTPVDSSEAKSGNKGAEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDEGNFVVDQMCELGKPEPLTEEQARGTAGNYDDEVSIRVGGNAQPSKVLNKKNVEGIGLLGGQKSKADYELYNKASNPDKVVGTPFTDNRNSETNDTTNGTHESDRNKESSDQTGVNMNGLENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL

Target ORF information:

RefSeq Version	XM_006502939.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus ubiquitin specific peptidase 1 (Usp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502939.3

ORF Insert Sequence:

ATGACCGGTG ACCAAGTTGT TCCTGCTGCA CAGTCCTCCC CAGTAAGCTG CGAGAAGAGA 
GAGAACTTGT TACCATTTGT GGGATTGAAT AACCTTGGCA ACACTTGTTA TCTGAATAGT 
ATTCTTCAGG TGTTATACTT TTGTCCTGGC TTTAAGACTG GAGTGAAGCA CTTATTTAAT 
ATTATTTCAA GGAAGAAAGA AGCTCTGAAG GATGACTCTA ATCAGAAGGA TAAGGGAAGC 
TGCAAAGAAG AGTCTTTGGC AAGTTATGAG CTTATATGTA GTTTACAGTC CTTAATAATT 
TCAGTTGAAC AGCTTCAGGC TAGTTTTCTC TTAAATCCAG AGAAATATAC AGATGAACTT 
GCTACACAGC CAAGGCGACT GCTGAACACA CTCAGGGAAC TCAACCCTAT GTATGAAGGA 
TTTCTACAGC ATGATGCACA GGAAGTGCTA CAGTGTATTC TGGGAAACAT TCAAGAAACA 
TGCCAACTCC TAAAAAAAGA AGAAATAAAA AACCTGGCTG AATTATCCGG CAAGGTTGAA 
GAACAATCTC TTCAGAAAGA GGAAACAGGT GGAATTACCA GCACAGAGAT TGACAGTATG 
AGGAATACGG AGGACGTCAA AGAGCAGCTC CCCAAAGGGA ACTGGAAAAG AAAAAGTGAC 
AGTGAGTCGA GTAACGTGAA GAAAAAGGTT AAACTGTCCA GGGAATCCCA GCCATTGGAA 
GAAAACCAGA GACAAACCAG ATCCAAGAGA AAAGCTATCG GAGACACACT AGAGGCTGCT 
CCTAAGATCA TCCCCAAGTG TGTTTCTGAA AGTGAGAGTG CAAAGCCCTC ACAGAAGAAA 
TCAAAAGTTA AAATAAATTG GTTAAAGCCA TCCACTAAGC AACCCAGCAT TCTTTCCAAG 
TTCTGTAGTC TTGGAAAAAT AACAACTAAC CAGCGCTCCA AAGGACAACC TAAGGAAAAG 
GAGGGTGATG TTGAAGAGGA CTTGGAGAAG TACGGAAGTG ACCACACAGC TAATGGTGGA 
CCCGAATCTC CAGGAAGTAG CGTCACACCT GTGGACAGCA GTGAAGCCAA GTCCGGAAAC 
AAAGGTGCTG AACAGATTGG CTTTGAGCTA GTAGAGAAAC TGTTTCAAGG TCAGCTCGTG 
TTAAGGACTC GGTGTTTGGA ATGTGAAAGC TTAACAGAAA GAAGAGAAGA TTTCCAGGAT 
ATTAGTGTTC CAGTGCAAGA AGATGAGCTT TCCAAGGTCG AGGAGAGCTC TGAAATTTCT 
CCAGAGCCAA AAACAGAAAT GAAGACCTTG AGATGGGCAA TTTCACAGTT TGCTTCAGTG 
GAGAGAATTG TAGGAGAGGA TAAATACTTC TGTGAAAATT GCCATCATTA TACGGAAGCA 
GAGCGAAGTC TCCTGTTTGA TAAAATGCCT GAAGTTATCA CTATTCATCT GAAGTGCTTT 
GCTGCTAGTG GCTTGGAGTT TGACTGTTAC GGTGGTGGAC TTTCCAAGAT CAACACTCCT 
TTATTGACAC CTCTTAAACT GTCACTAGAA GAATGGAGCA CAAAGCCGAC GAATGACAGC 
TATGGATTAT TTGCCGTTGT GATGCACAGT GGCATTACTA TTAGTAGTGG GCACTATACC 
GCATCTGTTA AAGTCACTGA CCTTAACAGT CTAGAGCTCG ATGAGGGGAA TTTTGTGGTT 
GACCAGATGT GTGAACTAGG TAAGCCAGAG CCACTGACCG AGGAGCAAGC AAGGGGGACG 
GCTGGGAACT ACGACGATGA AGTATCAATT AGAGTTGGTG GAAATGCCCA GCCAAGTAAA 
GTTTTGAACA AAAAAAATGT AGAAGGTATT GGACTTCTCG GTGGACAAAA GAGCAAAGCA 
GATTATGAGC TGTACAACAA AGCATCTAAT CCTGATAAGG TGGTTGGGAC ACCATTCACC 
GACAATAGAA ATTCTGAAAC TAATGATACT ACTAATGGGA CCCACGAATC TGACAGGAAC 
AAGGAATCCA GTGACCAAAC AGGCGTTAAC ATGAACGGAC TTGAGAACAA AATTTCCTAT 
GTAGTGCAAA GCTTAAAGGA GTACGAGGGG AAGTGGTTGC TCTTTGACGA TTCTGAGGTA 
AAAGTGACAG AAGAGAAAGA CTTTCTGAAT TCTCTCTCCC CTTCTACATC CCCTACGTCT 
ACTCCCTACT TGCTATTCTA TAAAAAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The role of USP1 autocleavage in DNA interstrand crosslink repair.
FEBS letters590(3)340-8(2016 Feb)
Kim M,Kim JM

A novel role for the deubiquitinase USP1 in the control of centrosome duplication.
Cell cycle (Georgetown, Tex.)15(4)584-92(2016)
Jung JK,Jang SW,Kim JM

Inactivation of Uaf1 causes defective homologous recombination and early embryonic lethality in mice.
Molecular and cellular biology33(22)4360-70(2013 Nov)
Park E,Kim JM,Primack B,Weinstock DM,Moreau LA,Parmar K,D'Andrea AD

CAPNS1 regulates USP1 stability and maintenance of genome integrity.
Molecular and cellular biology33(12)2485-96(2013 Jun)
Cataldo F,Peche LY,Klaric E,Brancolini C,Myers MP,Demarchi F,Schneider C

FANCD2 activates transcription of TAp63 and suppresses tumorigenesis.
Molecular cell50(6)908-18(2013 Jun)
Park E,Kim H,Kim JM,Primack B,Vidal-Cardenas S,Xu Y,Price BD,Mills AA,D'Andrea AD

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Usp1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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