Gtf2b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gtf2b
Entrez Gene ID	229906
Full Name	general transcription factor IIB
General protein information	Preferred Names general transcription factor IIB Names transcription initiation factor IIB RNA polymerase II alpha initiation factor general transcription factor TFIIB
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 H1

mRNA and Protein(s)

mRNA	Protein	Name
NM_145546.1	NP_663521.1	transcription initiation factor IIB

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GTF2B	NP_001039607.1
Rattus norvegicus (Norway rat)	Gtf2b	NP_112303.1
Danio rerio (zebrafish)	gtf2b	NP_955991.1
Eremothecium gossypii	AGOS_ADR007C	NP_984103.2
Pan troglodytes (chimpanzee)	GTF2B	XP_001147170.2
	GTF2B	XP_001083435.2
Canis lupus familiaris (dog)	GTF2B	XP_537085.1
Drosophila melanogaster (fruit fly)	TfIIB	NP_001260349.1
Caenorhabditis elegans (roundworm)	ttb-1	NP_503146.1
Kluyveromyces lactis	KLLA0D13640g	XP_453671.1
Gallus gallus (chicken)	GTF2B	XP_422356.3
Schizosaccharomyces pombe (fission yeast)	SPAC16E8.16	NP_594229.1
Neurospora crassa	NCU07289	XP_962143.1
Mus musculus (house mouse)	Gtf2b	NP_663521.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009558	XP_310128.4
Saccharomyces cerevisiae (baker's yeast)	SUA7	NP_015411.1
Arabidopsis thaliana (thale cress)	TFIIB	NP_181694.1
Oryza sativa (rice)	Os09g0534800	NP_001063775.1
Homo sapiens (human)	GTF2B	NP_001505.1
Magnaporthe oryzae (rice blast fungus)	MGG_05370	XP_003710216.1
Arabidopsis thaliana (thale cress)	AT3G10330	NP_187644.1
Xenopus tropicalis (tropical clawed frog)	gtf2b	NP_001016277.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The Specification of Cortical Subcerebral Projection Neurons Depends on the Direct Repression of TBR1 by CTIP1/BCL11a.
C?novas J, Berndt FA, Sep?lveda H, Aguilar R, Veloso FA, Montecino M, Oliva C, Maass JC, Sierralta J, Kukuljan M
The Journal of neuroscience : the official journal of the Society for Neuroscience35(19)7552-64(2015 May)

Acute targeting of general transcription factor IIB restricts cardiac hypertrophy via selective inhibition of gene transcription.
Sayed D, Yang Z, He M, Pfleger JM, Abdellatif M
Circulation. Heart failure8(1)138-48(2015 Jan)

A long noncoding RNA protects the heart from pathological hypertrophy.
Han P, Li W, Lin CH, Yang J, Shang C, Nuernberg ST, Jin KK, Xu W, Lin CY, Lin CJ, Xiong Y, Chien H, Zhou B, Ashley E, Bernstein D, Chen PS, Chen HV, Quertermous T, Chang CP
Nature514(7520)102-106(2014 Oct)

TAF4, a subunit of transcription factor II D, directs promoter occupancy of nuclear receptor HNF4A during post-natal hepatocyte differentiation.
Alpern D, Langer D, Ballester B, Le Gras S, Romier C, Mengus G, Davidson I
eLife3e03613(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

by acutely targeting TFIIB, we were able to inhibit selectively the former set of genes and ameliorate pressure overload hypertrophy.
Title: Acute targeting of general transcription factor IIB restricts cardiac hypertrophy via selective inhibition of gene transcription.

TFIIB co-localizes and interacts with alpha-tubulin during oocyte meiosis in the mouse and depletion of TFIIB causes arrest of subsequent embryo development.
Title: TFIIB co-localizes and interacts with ?-tubulin during oocyte meiosis in the mouse and depletion of TFIIB causes arrest of subsequent embryo development.

Study suggest that components of the transcription machinery decreased in accordance with cell differentiation and that TFIIB is specifically and rapidly degraded by the ubiquitin-proteasome pathway.
Title: Rapid proteasomal degradation of transcription factor IIB in accordance with F9 cell differentiation.

The following Gtf2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gtf2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11760	NM_145546.1 Latest version!	Mus musculus general transcription factor IIB (Gtf2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gtf2b ( NM_145546.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663521.1 Mus musculus general transcription factor IIB (Gtf2b), mRNA.

Price & Availability↑

CloneID	OMu11760
Clone ID Related Accession (Same CDS sequence)	NM_145546.1
Accession Version	NM_145546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	transcription initiation factor IIB
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC016637.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016637.1, AK131856.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCGA CCAGCCGTTT GGATGCTCTC CCAAGAGTCA CATGTCCGAA TCATCCAGAT 
GCAATTTTAG TTGAAGACTA CAGAGCTGGC GATATGATCT GTCCCGAATG TGGCCTAGTT 
GTAGGGGACC GAGTTATTGA TGTGGGATCT GAATGGAGAA CTTTCAGCAA TGACAAAGCA 
ACAAAAGACC CATCTAGAGT TGGAGACTCT CAGAATCCTC TTCTGAGTGA TGGAGATTTG 
TCCACCATGA TTGGCAAGGG TACAGGAGCT GCAAGTTTTG ATGAGTTTGG CAATTCTAAG 
TATCAGAACC GGAGAACAAT GAGTAGCTCT GATCGAGCAA TGATGAACGC ATTCAAGGAA 
ATCACGACCA TGGCGGACAG AATCAACCTC CCTCGCAATA TAGTTGATCG AACAAATAAT 
TTATTCAAGC AAGTGTATGA GCAGAAGAGC CTGAAGGGCA GAGCTAATGA CGCGATAGCC 
TCTGCTTGTC TGTACATCGC CTGTAGACAA GAAGGCGTTC CCAGGACATT TAAAGAAATA 
TGTGCTGTGT CTCGAATCTC TAAGAAAGAA ATTGGCCGCT GTTTTAAACT GATTTTGAAA 
GCTCTGGAAA CCAGCGTGGA TCTGATCACA ACTGGGGACT TCATGTCCAG GTTCTGCTCC 
AACCTTTGCC TCCCCAAGCA AGTGCAGATG GCAGCTACAC ACATAGCCCG CAAGGCAGTG 
GAGCTGGACT TGGTTCCTGG CAGGAGCCCG ATCTCTGTGG CGGCAGCAGC TATTTACATG 
GCTTCCCAGG CTTCAGCTGA GAAGCGAACA CAGAAAGAAA TCGGGGATAT TGCTGGTGTT 
GCTGATGTTA CAATCCGACA GTCCTACAGA CTGATCTACC CTCGGGCTCC GGATCTCTTC 
CCTTCAGACT TCAAGTTTGA CACCCCAGTG GACAAATTAC CCCAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663521.1
CDS	26..976
Translation	MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPSDFKFDTPVDKLPQL

Target ORF information:

RefSeq Version	NM_145546.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus general transcription factor IIB (Gtf2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145546.1

ORF Insert Sequence:

ATGGCGTCGA CCAGCCGTTT GGATGCTCTC CCAAGAGTCA CATGTCCGAA TCATCCAGAT 
GCAATTTTAG TTGAAGACTA CAGAGCTGGC GATATGATCT GTCCCGAATG TGGCCTAGTT 
GTAGGGGACC GAGTTATTGA TGTGGGATCT GAATGGAGAA CTTTCAGCAA TGACAAAGCA 
ACAAAAGACC CATCTAGAGT TGGAGACTCT CAGAATCCTC TTCTGAGTGA TGGAGATTTG 
TCCACCATGA TTGGCAAGGG TACAGGAGCT GCAAGTTTTG ATGAGTTTGG CAATTCTAAG 
TATCAGAACC GGAGAACAAT GAGTAGCTCT GATCGAGCAA TGATGAACGC ATTCAAGGAA 
ATCACGACCA TGGCGGACAG AATCAACCTC CCTCGCAATA TAGTTGATCG AACAAATAAT 
TTATTCAAGC AAGTGTATGA GCAGAAGAGC CTGAAGGGCA GAGCTAATGA CGCGATAGCC 
TCTGCTTGTC TGTACATCGC CTGTAGACAA GAAGGCGTTC CCAGGACATT TAAAGAAATA 
TGTGCTGTGT CTCGAATCTC TAAGAAAGAA ATTGGCCGCT GTTTTAAACT GATTTTGAAA 
GCTCTGGAAA CCAGCGTGGA TCTGATCACA ACTGGGGACT TCATGTCCAG GTTCTGCTCC 
AACCTTTGCC TCCCCAAGCA AGTGCAGATG GCAGCTACAC ACATAGCCCG CAAGGCAGTG 
GAGCTGGACT TGGTTCCTGG CAGGAGCCCG ATCTCTGTGG CGGCAGCAGC TATTTACATG 
GCTTCCCAGG CTTCAGCTGA GAAGCGAACA CAGAAAGAAA TCGGGGATAT TGCTGGTGTT 
GCTGATGTTA CAATCCGACA GTCCTACAGA CTGATCTACC CTCGGGCTCC GGATCTCTTC 
CCTTCAGACT TCAAGTTTGA CACCCCAGTG GACAAATTAC CCCAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The Specification of Cortical Subcerebral Projection Neurons Depends on the Direct Repression of TBR1 by CTIP1/BCL11a.
The Journal of neuroscience : the official journal of the Society for Neuroscience35(19)7552-64(2015 May)
C?novas J,Berndt FA,Sep?lveda H,Aguilar R,Veloso FA,Montecino M,Oliva C,Maass JC,Sierralta J,Kukuljan M

Acute targeting of general transcription factor IIB restricts cardiac hypertrophy via selective inhibition of gene transcription.
Circulation. Heart failure8(1)138-48(2015 Jan)
Sayed D,Yang Z,He M,Pfleger JM,Abdellatif M

A long noncoding RNA protects the heart from pathological hypertrophy.
Nature514(7520)102-106(2014 Oct)
Han P,Li W,Lin CH,Yang J,Shang C,Nuernberg ST,Jin KK,Xu W,Lin CY,Lin CJ,Xiong Y,Chien H,Zhou B,Ashley E,Bernstein D,Chen PS,Chen HV,Quertermous T,Chang CP

TAF4, a subunit of transcription factor II D, directs promoter occupancy of nuclear receptor HNF4A during post-natal hepatocyte differentiation.
eLife3e03613(2014 Sep)
Alpern D,Langer D,Ballester B,Le Gras S,Romier C,Mengus G,Davidson I

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Gtf2b cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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