GTF2B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GTF2B
Entrez Gene ID	2959
Full Name	general transcription factor IIB
Synonyms	TF2B,TFIIB
General protein information	Preferred Names general transcription factor IIB Names transcription initiation factor IIB RNA polymerase II transcription factor IIB S300-II general transcription factor TFIIB
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p22.2
Summary	This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008].
Disorder MIM:	189963

mRNA and Protein(s)

mRNA	Protein	Name
NM_001514.5	NP_001505.1	transcription initiation factor IIB
NM_001514.6	NP_001505.1	transcription initiation factor IIB
XM_011541299.1	XP_011539601.1	transcription initiation factor IIB isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GTF2B	NP_001039607.1
Rattus norvegicus (Norway rat)	Gtf2b	NP_112303.1
Danio rerio (zebrafish)	gtf2b	NP_955991.1
Eremothecium gossypii	AGOS_ADR007C	NP_984103.2
Pan troglodytes (chimpanzee)	GTF2B	XP_001147170.2
	GTF2B	XP_001083435.2
Canis lupus familiaris (dog)	GTF2B	XP_537085.1
Drosophila melanogaster (fruit fly)	TfIIB	NP_001260349.1
Caenorhabditis elegans (roundworm)	ttb-1	NP_503146.1
Kluyveromyces lactis	KLLA0D13640g	XP_453671.1
Gallus gallus (chicken)	GTF2B	XP_422356.3
Schizosaccharomyces pombe (fission yeast)	SPAC16E8.16	NP_594229.1
Neurospora crassa	NCU07289	XP_962143.1
Mus musculus (house mouse)	Gtf2b	NP_663521.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009558	XP_310128.4
Saccharomyces cerevisiae (baker's yeast)	SUA7	NP_015411.1
Arabidopsis thaliana (thale cress)	TFIIB	NP_181694.1
Oryza sativa (rice)	Os09g0534800	NP_001063775.1
Homo sapiens (human)	GTF2B	NP_001505.1
Magnaporthe oryzae (rice blast fungus)	MGG_05370	XP_003710216.1
Arabidopsis thaliana (thale cress)	AT3G10330	NP_187644.1
Xenopus tropicalis (tropical clawed frog)	gtf2b	NP_001016277.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural dissection of an interaction between transcription initiation and termination factors implicated in promoter-terminator crosstalk.
Bratkowski M, Unarta IC, Zhu L, Shubbar M, Huang X, Liu X
The Journal of biological chemistry(2017 Nov)

Near-atomic resolution visualization of human transcription promoter opening.
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature533(7603)359-65(2016 05)

Evaluation of the Intrinsic Zn(II) Affinity of a Cys3His1 Site in the Absence of Protein Folding Effects.
Reddi AR, Pawlowska M, Gibney BR
Inorganic chemistry54(12)5942-8(2015 Jun)

Association of the winged helix motif of the TFIIE? subunit of TFIIE with either the TFIIE? subunit or TFIIB distinguishes its functions in transcription.
Tanaka A, Akimoto Y, Kobayashi S, Hisatake K, Hanaoka F, Ohkuma Y
Genes to cells : devoted to molecular & cellular mechanisms20(3)203-16(2015 Mar)

In vitro fluorescence studies of transcription factor IIB-DNA interaction.
G?recki A, Figiel M, Dziedzicka-Wasylewska M
Acta biochimica Polonica62(3)413-21(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The role of zinc at Cys3His1 binding site in the absence of TFIIB protein folding
Title: Evaluation of the Intrinsic Zn(II) Affinity of a Cys3His1 Site in the Absence of Protein Folding Effects.

Association of the winged helix motif of the TFIIEalpha subunit of TFIIE with either the TFIIEbeta subunit or TFIIB distinguishes its functions in transcription.
Title: Association of the winged helix motif of the TFIIE? subunit of TFIIE with either the TFIIE? subunit or TFIIB distinguishes its functions in transcription.

binding of specific DNA sequences changes the protein structure and dynamics, and TFIIB may exist in two conformational states
Title: In vitro fluorescence studies of transcription factor IIB-DNA interaction.

these results establish a new paradigm for TFIIB functionality in human gene expression, which when downregulated has potent anti-viral effects.
Title: A new paradigm for transcription factor TFIIB functionality.

a mode of phospho-TFIIB-independent transcriptional regulation that prioritizes the transcription of p53-target genes during cellular stress.
Title: TFIIB dephosphorylation links transcription inhibition with the p53-dependent DNA damage response.

The following GTF2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GTF2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02972	NM_001514.5 Latest version!	Homo sapiens general transcription factor IIB (GTF2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu58147	XM_011541299.1 Latest version!	Homo sapiens general transcription factor IIB (GTF2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu107396	NM_001514.6 Latest version!	Homo sapiens general transcription factor IIB (GTF2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GTF2B ( NM_001514.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001505.1 Homo sapiens general transcription factor IIB (GTF2B), mRNA.

Price & Availability↑

CloneID	OHu02972
Clone ID Related Accession (Same CDS sequence)	NM_001514.5
Accession Version	NM_001514.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	transcription initiation factor IIB
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP379342.1, BC020597.1 and AA031701.1. On Mar 4, 2008 this sequence version replaced NM_001514.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.206666.1, SRR1803613.210210.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCTA CCAGCCGTTT GGATGCTCTT CCAAGAGTCA CATGTCCAAA CCATCCAGAT 
GCGATTTTAG TGGAGGACTA CAGAGCCGGT GATATGATCT GTCCTGAATG TGGCTTGGTT 
GTAGGTGACC GGGTTATTGA TGTGGGATCT GAATGGCGAA CTTTCAGCAA TGACAAAGCA 
ACAAAAGATC CATCTCGAGT TGGAGATTCT CAGAATCCTC TTCTGAGTGA TGGAGATTTG 
TCTACCATGA TTGGCAAGGG CACAGGAGCT GCAAGTTTTG ACGAATTTGG CAATTCTAAG 
TACCAGAATC GGAGAACAAT GAGCAGTTCT GATCGGGCAA TGATGAATGC ATTCAAAGAA 
ATCACTACCA TGGCAGACAG AATCAATCTA CCTCGAAATA TAGTTGATCG AACAAATAAT 
TTATTCAAGC AAGTATATGA ACAGAAGAGC CTGAAGGGAA GAGCTAATGA TGCTATAGCT 
TCTGCTTGTC TCTATATTGC CTGTAGACAA GAAGGGGTTC CTAGGACATT TAAAGAAATA 
TGTGCCGTAT CACGAATTTC TAAGAAAGAA ATTGGTCGGT GTTTTAAACT TATTTTGAAA 
GCGCTAGAAA CCAGTGTGGA TTTGATTACA ACTGGGGACT TCATGTCCAG GTTCTGTTCC 
AACCTTTGTC TTCCTAAACA AGTACAGATG GCAGCTACAC ATATAGCCCG TAAAGCTGTG 
GAATTGGACT TGGTTCCTGG GAGGAGCCCC ATCTCTGTGG CAGCGGCAGC TATTTACATG 
GCCTCACAGG CATCAGCTGA AAAGAGGACC CAAAAAGAAA TTGGAGATAT TGCTGGTGTT 
GCTGATGTTA CAATCAGACA GTCCTATAGA CTGATCTATC CTCGAGCCCC AGATCTGTTT 
CCTACAGACT TCAAATTTGA CACCCCAGTG GACAAACTAC CACAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001505.1
CDS	120..1070
Translation	MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL

Target ORF information:

RefSeq Version	NM_001514.5
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIB (GTF2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001514.5

ORF Insert Sequence:

ATGGCGTCTA CCAGCCGTTT GGATGCTCTT CCAAGAGTCA CATGTCCAAA CCATCCAGAT 
GCGATTTTAG TGGAGGACTA CAGAGCCGGT GATATGATCT GTCCTGAATG TGGCTTGGTT 
GTAGGTGACC GGGTTATTGA TGTGGGATCT GAATGGCGAA CTTTCAGCAA TGACAAAGCA 
ACAAAAGATC CATCTCGAGT TGGAGATTCT CAGAATCCTC TTCTGAGTGA TGGAGATTTG 
TCTACCATGA TTGGCAAGGG CACAGGAGCT GCAAGTTTTG ACGAATTTGG CAATTCTAAG 
TACCAGAATC GGAGAACAAT GAGCAGTTCT GATCGGGCAA TGATGAATGC ATTCAAAGAA 
ATCACTACCA TGGCAGACAG AATCAATCTA CCTCGAAATA TAGTTGATCG AACAAATAAT 
TTATTCAAGC AAGTATATGA ACAGAAGAGC CTGAAGGGAA GAGCTAATGA TGCTATAGCT 
TCTGCTTGTC TCTATATTGC CTGTAGACAA GAAGGGGTTC CTAGGACATT TAAAGAAATA 
TGTGCCGTAT CACGAATTTC TAAGAAAGAA ATTGGTCGGT GTTTTAAACT TATTTTGAAA 
GCGCTAGAAA CCAGTGTGGA TTTGATTACA ACTGGGGACT TCATGTCCAG GTTCTGTTCC 
AACCTTTGTC TTCCTAAACA AGTACAGATG GCAGCTACAC ATATAGCCCG TAAAGCTGTG 
GAATTGGACT TGGTTCCTGG GAGGAGCCCC ATCTCTGTGG CAGCGGCAGC TATTTACATG 
GCCTCACAGG CATCAGCTGA AAAGAGGACC CAAAAAGAAA TTGGAGATAT TGCTGGTGTT 
GCTGATGTTA CAATCAGACA GTCCTATAGA CTGATCTATC CTCGAGCCCC AGATCTGTTT 
CCTACAGACT TCAAATTTGA CACCCCAGTG GACAAACTAC CACAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTF2B ( XM_011541299.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539601.1 Homo sapiens general transcription factor IIB (GTF2B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu58147
Clone ID Related Accession (Same CDS sequence)	XM_011541299.1
Accession Version	XM_011541299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription initiation factor IIB isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTC CTGAATGTGG CTTGGTTGTA GGTGACCGGG TTATTGATGT GGGATCTGAA 
TGGCGAACTT TCAGCAATGA CAAAGCAACA AAAGATCCAT CTCGAGTTGG AGATTCTCAG 
AATCCTCTTC TGAGTGATGG AGATTTGTCT ACCATGATTG GCAAGGGCAC AGGAGCTGCA 
AGTTTTGACG AATTTGGCAA TTCTAAGTAC CAGAATCGGA GAACAATGAG CAGTTCTGAT 
CGGGCAATGA TGAATGCATT CAAAGAAATC ACTACCATGG CAGACAGAAT CAATCTACCT 
CGAAATATAG TTGATCGAAC AAATAATTTA TTCAAGCAAG TATATGAACA GAAGAGCCTG 
AAGGGAAGAG CTAATGATGC TATAGCTTCT GCTTGTCTCT ATATTGCCTG TAGACAAGAA 
GGGGTTCCTA GGACATTTAA AGAAATATGT GCCGTATCAC GAATTTCTAA GAAAGAAATT 
GGTCGGTGTT TTAAACTTAT TTTGAAAGCG CTAGAAACCA GTGTGGATTT GATTACAACT 
GGGGACTTCA TGTCCAGGTT CTGTTCCAAC CTTTGTCTTC CTAAACAAGT ACAGATGGCA 
GCTACACATA TAGCCCGTAA AGCTGTGGAA CTGGACTTGG TTCCTGGGAG GAGCCCCATC 
TCTGTGGCAG CGGCAGCTAT TTACATGGCC TCACAGGCAT CAGCTGAAAA GAGGACCCAA 
AAAGAAATTG GAGATATTGC TGGTGTTGCT GATGTTACAA TCAGACAGTC CTATAGACTG 
ATCTATCCTC GAGCCCCAGA TCTGTTTCCT ACAGACTTCA AATTTGACAC CCCAGTGGAC 
AAACTACCAC AGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539601.1
CDS	415..1272
Translation	MICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL

Target ORF information:

RefSeq Version	XM_011541299.1
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIB (GTF2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541299.1

ORF Insert Sequence:

ATGATCTGTC CTGAATGTGG CTTGGTTGTA GGTGACCGGG TTATTGATGT GGGATCTGAA 
TGGCGAACTT TCAGCAATGA CAAAGCAACA AAAGATCCAT CTCGAGTTGG AGATTCTCAG 
AATCCTCTTC TGAGTGATGG AGATTTGTCT ACCATGATTG GCAAGGGCAC AGGAGCTGCA 
AGTTTTGACG AATTTGGCAA TTCTAAGTAC CAGAATCGGA GAACAATGAG CAGTTCTGAT 
CGGGCAATGA TGAATGCATT CAAAGAAATC ACTACCATGG CAGACAGAAT CAATCTACCT 
CGAAATATAG TTGATCGAAC AAATAATTTA TTCAAGCAAG TATATGAACA GAAGAGCCTG 
AAGGGAAGAG CTAATGATGC TATAGCTTCT GCTTGTCTCT ATATTGCCTG TAGACAAGAA 
GGGGTTCCTA GGACATTTAA AGAAATATGT GCCGTATCAC GAATTTCTAA GAAAGAAATT 
GGTCGGTGTT TTAAACTTAT TTTGAAAGCG CTAGAAACCA GTGTGGATTT GATTACAACT 
GGGGACTTCA TGTCCAGGTT CTGTTCCAAC CTTTGTCTTC CTAAACAAGT ACAGATGGCA 
GCTACACATA TAGCCCGTAA AGCTGTGGAA CTGGACTTGG TTCCTGGGAG GAGCCCCATC 
TCTGTGGCAG CGGCAGCTAT TTACATGGCC TCACAGGCAT CAGCTGAAAA GAGGACCCAA 
AAAGAAATTG GAGATATTGC TGGTGTTGCT GATGTTACAA TCAGACAGTC CTATAGACTG 
ATCTATCCTC GAGCCCCAGA TCTGTTTCCT ACAGACTTCA AATTTGACAC CCCAGTGGAC 
AAACTACCAC AGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTF2B ( NM_001514.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_001505.1 Homo sapiens general transcription factor IIB (GTF2B), mRNA.

Price & Availability↑

CloneID	OHu107396
Clone ID Related Accession (Same CDS sequence)	NM_001514.6
Accession Version	NM_001514.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	transcription initiation factor IIB
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL445991.15. On Nov 22, 2018 this sequence version replaced NM_001514.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.635150.1, SRR1660803.151351.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000370500.10/ ENSP00000359531.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTCTA CCAGCCGTTT GGATGCTCTT CCAAGAGTCA CATGTCCAAA CCATCCAGAT 
GCGATTTTAG TGGAGGACTA CAGAGCCGGT GATATGATCT GTCCTGAATG TGGCTTGGTT 
GTAGGTGACC GGGTTATTGA TGTGGGATCT GAATGGCGAA CTTTCAGCAA TGACAAAGCA 
ACAAAAGATC CATCTCGAGT TGGAGATTCT CAGAATCCTC TTCTGAGTGA TGGAGATTTG 
TCTACCATGA TTGGCAAGGG CACAGGAGCT GCAAGTTTTG ACGAATTTGG CAATTCTAAG 
TACCAGAATC GGAGAACAAT GAGCAGTTCT GATCGGGCAA TGATGAATGC ATTCAAAGAA 
ATCACTACCA TGGCAGACAG AATCAATCTA CCTCGAAATA TAGTTGATCG AACAAATAAT 
TTATTCAAGC AAGTATATGA ACAGAAGAGC CTGAAGGGAA GAGCTAATGA TGCTATAGCT 
TCTGCTTGTC TCTATATTGC CTGTAGACAA GAAGGGGTTC CTAGGACATT TAAAGAAATA 
TGTGCCGTAT CACGAATTTC TAAGAAAGAA ATTGGTCGGT GTTTTAAACT TATTTTGAAA 
GCGCTAGAAA CCAGTGTGGA TTTGATTACA ACTGGGGACT TCATGTCCAG GTTCTGTTCC 
AACCTTTGTC TTCCTAAACA AGTACAGATG GCAGCTACAC ATATAGCCCG TAAAGCTGTG 
GAACTGGACT TGGTTCCTGG GAGGAGCCCC ATCTCTGTGG CAGCGGCAGC TATTTACATG 
GCCTCACAGG CATCAGCTGA AAAGAGGACC CAAAAAGAAA TTGGAGATAT TGCTGGTGTT 
GCTGATGTTA CAATCAGACA GTCCTATAGA CTGATCTATC CTCGAGCCCC AGATCTGTTT 
CCTACAGACT TCAAATTTGA CACCCCAGTG GACAAACTAC CACAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001505.1
CDS	69..1019
Translation	MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL

Target ORF information:

RefSeq Version	NM_001514.6
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIB (GTF2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001514.6

ORF Insert Sequence:

ATGGCGTCTA CCAGCCGTTT GGATGCTCTT CCAAGAGTCA CATGTCCAAA CCATCCAGAT 
GCGATTTTAG TGGAGGACTA CAGAGCCGGT GATATGATCT GTCCTGAATG TGGCTTGGTT 
GTAGGTGACC GGGTTATTGA TGTGGGATCT GAATGGCGAA CTTTCAGCAA TGACAAAGCA 
ACAAAAGATC CATCTCGAGT TGGAGATTCT CAGAATCCTC TTCTGAGTGA TGGAGATTTG 
TCTACCATGA TTGGCAAGGG CACAGGAGCT GCAAGTTTTG ACGAATTTGG CAATTCTAAG 
TACCAGAATC GGAGAACAAT GAGCAGTTCT GATCGGGCAA TGATGAATGC ATTCAAAGAA 
ATCACTACCA TGGCAGACAG AATCAATCTA CCTCGAAATA TAGTTGATCG AACAAATAAT 
TTATTCAAGC AAGTATATGA ACAGAAGAGC CTGAAGGGAA GAGCTAATGA TGCTATAGCT 
TCTGCTTGTC TCTATATTGC CTGTAGACAA GAAGGGGTTC CTAGGACATT TAAAGAAATA 
TGTGCCGTAT CACGAATTTC TAAGAAAGAA ATTGGTCGGT GTTTTAAACT TATTTTGAAA 
GCGCTAGAAA CCAGTGTGGA TTTGATTACA ACTGGGGACT TCATGTCCAG GTTCTGTTCC 
AACCTTTGTC TTCCTAAACA AGTACAGATG GCAGCTACAC ATATAGCCCG TAAAGCTGTG 
GAACTGGACT TGGTTCCTGG GAGGAGCCCC ATCTCTGTGG CAGCGGCAGC TATTTACATG 
GCCTCACAGG CATCAGCTGA AAAGAGGACC CAAAAAGAAA TTGGAGATAT TGCTGGTGTT 
GCTGATGTTA CAATCAGACA GTCCTATAGA CTGATCTATC CTCGAGCCCC AGATCTGTTT 
CCTACAGACT TCAAATTTGA CACCCCAGTG GACAAACTAC CACAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural dissection of an interaction between transcription initiation and termination factors implicated in promoter-terminator crosstalk.
The Journal of biological chemistry(2017 Nov)
Bratkowski M,Unarta IC,Zhu L,Shubbar M,Huang X,Liu X

Near-atomic resolution visualization of human transcription promoter opening.
Nature533(7603)359-65(2016 05)
He Y,Yan C,Fang J,Inouye C,Tjian R,Ivanov I,Nogales E

Evaluation of the Intrinsic Zn(II) Affinity of a Cys3His1 Site in the Absence of Protein Folding Effects.
Inorganic chemistry54(12)5942-8(2015 Jun)
Reddi AR,Pawlowska M,Gibney BR

Association of the winged helix motif of the TFIIE? subunit of TFIIE with either the TFIIE? subunit or TFIIB distinguishes its functions in transcription.
Genes to cells : devoted to molecular & cellular mechanisms20(3)203-16(2015 Mar)
Tanaka A,Akimoto Y,Kobayashi S,Hisatake K,Hanaoka F,Ohkuma Y

In vitro fluorescence studies of transcription factor IIB-DNA interaction.
Acta biochimica Polonica62(3)413-21(2015)
G?recki A,Figiel M,Dziedzicka-Wasylewska M

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GTF2B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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