Ampd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ampd1
Entrez Gene ID	229665
Full Name	adenosine monophosphate deaminase 1
Synonyms	AI553520,Ampd-1
General protein information	Preferred Names adenosine monophosphate deaminase 1 Names AMP deaminase 1 adenosine monophosphate deaminase 1 (isoform M) myoadenylate deaminase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 F2.2\|3 45.25 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006501401.2	XP_006501464.1	AMP deaminase 1 isoform X2
NM_001033303.2	NP_001028475.2	AMP deaminase 1
XM_006501401.3	XP_006501464.1	AMP deaminase 1 isoform X2
XM_006501400.3	XP_006501463.1	AMP deaminase 1 isoform X1
XM_006501400.4	XP_006501463.1	AMP deaminase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	AMPD1	NP_000027.2
Danio rerio (zebrafish)	ampd1	NP_957187.1
	AMPD1	XP_001111578.2
Xenopus tropicalis (tropical clawed frog)	ampd1	XP_002935821.2
Pan troglodytes (chimpanzee)	AMPD1	XP_513671.3
Mus musculus (house mouse)	Ampd1	NP_001028475.2
Gallus gallus (chicken)	AMPD1	XP_003642776.1
Bos taurus (cattle)	AMPD1	NP_001093819.1
Rattus norvegicus (Norway rat)	Ampd1	NP_620231.1
Canis lupus familiaris (dog)	AMPD1	XP_848536.2

Gene Ontology
Bibliography

Related articles in PubMed

Insertion of a knockout-first cassette in Ampd1 gene leads to neonatal death by disruption of neighboring genes expression.
Pan Y, Zhang L, Liu Q, Li Y, Guo H, Peng Y, Peng H, Tang B, Hu Z, Zhao J, Xia K, Li JD
Scientific reports635970(2016 Oct)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

AMPD1 regulates mTORC1-p70 S6 kinase axis in the control of insulin sensitivity in skeletal muscle.
Tandelilin AA, Hirase T, Hudoyo AW, Cheng J, Toyama K, Morisaki H, Morisaki T
BMC endocrine disorders1511(2015 Mar)

AMPD1: a novel therapeutic target for reversing insulin resistance.
Cheng J, Morisaki H, Toyama K, Sugimoto N, Shintani T, Tandelilin A, Hirase T, Holmes EW, Morisaki T
BMC endocrine disorders1496(2014 Dec)

Effect of isolated AMP deaminase deficiency on skeletal muscle function.
Cheng J, Morisaki H, Sugimoto N, Dohi A, Shintani T, Kimura E, Toyama K, Ikawa M, Okabe M, Higuchi I, Matsuo S, Kawai Y, Hisatome I, Sugama T, Holmes EW, Morisaki T
Molecular genetics and metabolism reports151-59(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AMPD1 deficiency activates AMPK/Akt/mTORC1/p70 S6 kinase axis in skeletal muscle after high fat diet challenge, but not in normal chow diet. These changes may contribute to improve insulin resistance.
Title: AMPD1 regulates mTORC1-p70 S6 kinase axis in the control of insulin sensitivity in skeletal muscle.

Disruption of the AMPD1 gene leads to a less severe state of insulin resistance, improved glucose tolerance and enhanced insulin clearance in mice fed a high-fat diet. Data suggest that AMPD may be a new drug target for reversing insulin resistance.
Title: AMPD1: a novel therapeutic target for reversing insulin resistance.

AMPD1 deficiency is acquired prior to overt muscle inflammation and is responsible, at least in part, for the muscle weakness that occurs in the mouse model of myositis.
Title: The molecular basis of skeletal muscle weakness in a mouse model of inflammatory myopathy.

The following Ampd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ampd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu31858	XM_006501401.3 Latest version!	Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu20693	NM_001033303.2 Latest version!	Mus musculus adenosine monophosphate deaminase 1 (Ampd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu31857	XM_006501400.4 Latest version!	Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu31858	XM_006501401.2	Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu31857	XM_006501400.3	Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ampd1 ( XM_006501401.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501464.1 Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu31858
Clone ID Related Accession (Same CDS sequence)	XM_006501401.2 , XM_006501401.3
Accession Version	XM_006501401.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	AMP deaminase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA AGCACCTGCT GCGCTTCATT AAGAAATCTT ACCATATTGA CGCCGACAGG 
GTGGTCTACA GCACTAAAGA GAAGAGCCTG ACTCTGAAGG AACTTTTCGC TAAGTTGAAT 
ATGCATCCCT ATGACCTGAC TGTTGACTCT CTGGATGTTC ATGCTGGACG CCAGACGTTC 
CAACGTTTTG ATAAGTTCAA TGACAAATAC AATCCCGTGG GTGCAAGTGA GCTTCGGGAC 
CTCTACCTAA AAACAGACAA CTACATTAAT GGGGAGTATT TTGCCACTAT CATCAAGGAG 
GTGGGTGCAG ACCTGGTGGA GGCCAAGTAT CAGCATGCAG AGCCTCGCTT ATCCATCTAC 
GGTCGCAGTC CAGATGAGTG GAACAAACTC TCCTCTTGGT TTGTCTGCAA TCGCATCTAT 
TGCCCCAACA TGACGTGGAT GATCCAGGTC CCCAGGATCT ATGATGTGTT CCGATCCAAG 
AATTTCCTGC CACACTTCGG AAAGATGCTG GAGAATATTT TCCTTCCAGT GTTTGAGGCC 
ACCATCAACC CCCAAGCTCA CCCAGACCTC AGTGTCTTCC TCAAGCACAT CACTGGCTTT 
GACAGTGTGG ATGATGAATC CAAACACAGT GGTCACATGT TTTCCTCCAA GAGTCCCAAA 
CCCGAGGAGT GGACAATGGA AAACAACCCG TCTTATACTT ACTATGCCTA CTACATGTAT 
GCAAACATCA CGGTGCTCAA CAGCCTGAGA AAGGAACGAG GCATGAATAC ATTTCTGTTT 
CGTCCCCACT GTGGGGAGGC TGGGGCTCTC ACTCACCTCA TGACAGCCTT CATGATAGCA 
GACAATATTT CTCATGGACT GAATTTAAAG AAGAGTCCTG TGCTACAGTA CTTGTTTTTC 
CTAGCCCAGA TTCCCATCGC CATGTCACCG CTGAGTAACA ACAGTCTGTT CCTCGAATAT 
GCAAAGAATC CTTTCTTAGA TTTCCTCCAG AAAGGCCTGA TGATCTCACT GTCGACCGAT 
GACCCGATGC AGTTCCACTT CACCAAGGAG CCCCTGATGG AAGAATACGC CATCGCTGCG 
CAAGTCTTCA AGCTGAGCAC GTGTGACATG TGTGAGGTGG CAAGGAACAG TGTCCTGCAG 
TGTGGGATTT CTCATGAGGA AAAAGCAAAG TTTCTGGGCA ACAATTACCT TGAGGAAGGC 
CCTGTTGGAA ATGACATCCG GAGGACAAAT GTCGCTCAGA TTCGCATGGC CTATCGTTAT 
GAAACTTGGT GTTATGAACT CAATTTAATT GCTGAGGGTC TTAAAGCAAC AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501464.1
CDS	130..1446
Translation	MNQKHLLRFIKKSYHIDADRVVYSTKEKSLTLKELFAKLNMHPYDLTVDSLDVHAGRQTFQRFDKFNDKYNPVGASELRDLYLKTDNYINGEYFATIIKEVGADLVEAKYQHAEPRLSIYGRSPDEWNKLSSWFVCNRIYCPNMTWMIQVPRIYDVFRSKNFLPHFGKMLENIFLPVFEATINPQAHPDLSVFLKHITGFDSVDDESKHSGHMFSSKSPKPEEWTMENNPSYTYYAYYMYANITVLNSLRKERGMNTFLFRPHCGEAGALTHLMTAFMIADNISHGLNLKKSPVLQYLFFLAQIPIAMSPLSNNSLFLEYAKNPFLDFLQKGLMISLSTDDPMQFHFTKEPLMEEYAIAAQVFKLSTCDMCEVARNSVLQCGISHEEKAKFLGNNYLEEGPVGNDIRRTNVAQIRMAYRYETWCYELNLIAEGLKATE

Target ORF information:

RefSeq Version	XM_006501401.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501401.2

ORF Insert Sequence:

ATGAACCAGA AGCACCTGCT GCGCTTCATT AAGAAATCTT ACCATATTGA CGCCGACAGG 
GTGGTCTACA GCACTAAAGA GAAGAGCCTG ACTCTGAAGG AACTTTTCGC TAAGTTGAAT 
ATGCATCCCT ATGACCTGAC TGTTGACTCT CTGGATGTTC ATGCTGGACG CCAGACGTTC 
CAACGTTTTG ATAAGTTCAA TGACAAATAC AATCCCGTGG GTGCAAGTGA GCTTCGGGAC 
CTCTACCTAA AAACAGACAA CTACATTAAT GGGGAGTATT TTGCCACTAT CATCAAGGAG 
GTGGGTGCAG ACCTGGTGGA GGCCAAGTAT CAGCATGCAG AGCCTCGCTT ATCCATCTAC 
GGTCGCAGTC CAGATGAGTG GAACAAACTC TCCTCTTGGT TTGTCTGCAA TCGCATCTAT 
TGCCCCAACA TGACGTGGAT GATCCAGGTC CCCAGGATCT ATGATGTGTT CCGATCCAAG 
AATTTCCTGC CACACTTCGG AAAGATGCTG GAGAATATTT TCCTTCCAGT GTTTGAGGCC 
ACCATCAACC CCCAAGCTCA CCCAGACCTC AGTGTCTTCC TCAAGCACAT CACTGGCTTT 
GACAGTGTGG ATGATGAATC CAAACACAGT GGTCACATGT TTTCCTCCAA GAGTCCCAAA 
CCCGAGGAGT GGACAATGGA AAACAACCCG TCTTATACTT ACTATGCCTA CTACATGTAT 
GCAAACATCA CGGTGCTCAA CAGCCTGAGA AAGGAACGAG GCATGAATAC ATTTCTGTTT 
CGTCCCCACT GTGGGGAGGC TGGGGCTCTC ACTCACCTCA TGACAGCCTT CATGATAGCA 
GACAATATTT CTCATGGACT GAATTTAAAG AAGAGTCCTG TGCTACAGTA CTTGTTTTTC 
CTAGCCCAGA TTCCCATCGC CATGTCACCG CTGAGTAACA ACAGTCTGTT CCTCGAATAT 
GCAAAGAATC CTTTCTTAGA TTTCCTCCAG AAAGGCCTGA TGATCTCACT GTCGACCGAT 
GACCCGATGC AGTTCCACTT CACCAAGGAG CCCCTGATGG AAGAATACGC CATCGCTGCG 
CAAGTCTTCA AGCTGAGCAC GTGTGACATG TGTGAGGTGG CAAGGAACAG TGTCCTGCAG 
TGTGGGATTT CTCATGAGGA AAAAGCAAAG TTTCTGGGCA ACAATTACCT TGAGGAAGGC 
CCTGTTGGAA ATGACATCCG GAGGACAAAT GTCGCTCAGA TTCGCATGGC CTATCGTTAT 
GAAACTTGGT GTTATGAACT CAATTTAATT GCTGAGGGTC TTAAAGCAAC AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ampd1 ( XM_006501401.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501464.1 Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu31858
Clone ID Related Accession (Same CDS sequence)	XM_006501401.2 , XM_006501401.3
Accession Version	XM_006501401.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AMP deaminase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501401.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA AGCACCTGCT GCGCTTCATT AAGAAATCTT ACCATATTGA CGCCGACAGG 
GTGGTCTACA GCACTAAAGA GAAGAGCCTG ACTCTGAAGG AACTTTTCGC TAAGTTGAAT 
ATGCATCCCT ATGACCTGAC TGTTGACTCT CTGGATGTTC ATGCTGGACG CCAGACGTTC 
CAACGTTTTG ATAAGTTCAA TGACAAATAC AATCCCGTGG GTGCAAGTGA GCTTCGGGAC 
CTCTACCTAA AAACAGACAA CTACATTAAT GGGGAGTATT TTGCCACTAT CATCAAGGAG 
GTGGGTGCAG ACCTGGTGGA GGCCAAGTAT CAGCATGCAG AGCCTCGCTT ATCCATCTAC 
GGTCGCAGTC CAGATGAGTG GAACAAACTC TCCTCTTGGT TTGTCTGCAA TCGCATCTAT 
TGCCCCAACA TGACGTGGAT GATCCAGGTC CCCAGGATCT ATGATGTGTT CCGATCCAAG 
AATTTCCTGC CACACTTCGG AAAGATGCTG GAGAATATTT TCCTTCCAGT GTTTGAGGCC 
ACCATCAACC CCCAAGCTCA CCCAGACCTC AGTGTCTTCC TCAAGCACAT CACTGGCTTT 
GACAGTGTGG ATGATGAATC CAAACACAGT GGTCACATGT TTTCCTCCAA GAGTCCCAAA 
CCCGAGGAGT GGACAATGGA AAACAACCCG TCTTATACTT ACTATGCCTA CTACATGTAT 
GCAAACATCA CGGTGCTCAA CAGCCTGAGA AAGGAACGAG GCATGAATAC ATTTCTGTTT 
CGTCCCCACT GTGGGGAGGC TGGGGCTCTC ACTCACCTCA TGACAGCCTT CATGATAGCA 
GACAATATTT CTCATGGACT GAATTTAAAG AAGAGTCCTG TGCTACAGTA CTTGTTTTTC 
CTAGCCCAGA TTCCCATCGC CATGTCACCG CTGAGTAACA ACAGTCTGTT CCTCGAATAT 
GCAAAGAATC CTTTCTTAGA TTTCCTCCAG AAAGGCCTGA TGATCTCACT GTCGACCGAT 
GACCCGATGC AGTTCCACTT CACCAAGGAG CCCCTGATGG AAGAATACGC CATCGCTGCG 
CAAGTCTTCA AGCTGAGCAC GTGTGACATG TGTGAGGTGG CAAGGAACAG TGTCCTGCAG 
TGTGGGATTT CTCATGAGGA AAAAGCAAAG TTTCTGGGCA ACAATTACCT TGAGGAAGGC 
CCTGTTGGAA ATGACATCCG GAGGACAAAT GTCGCTCAGA TTCGCATGGC CTATCGTTAT 
GAAACTTGGT GTTATGAACT CAATTTAATT GCTGAGGGTC TTAAAGCAAC AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501464.1
CDS	161..1477
Translation	MNQKHLLRFIKKSYHIDADRVVYSTKEKSLTLKELFAKLNMHPYDLTVDSLDVHAGRQTFQRFDKFNDKYNPVGASELRDLYLKTDNYINGEYFATIIKEVGADLVEAKYQHAEPRLSIYGRSPDEWNKLSSWFVCNRIYCPNMTWMIQVPRIYDVFRSKNFLPHFGKMLENIFLPVFEATINPQAHPDLSVFLKHITGFDSVDDESKHSGHMFSSKSPKPEEWTMENNPSYTYYAYYMYANITVLNSLRKERGMNTFLFRPHCGEAGALTHLMTAFMIADNISHGLNLKKSPVLQYLFFLAQIPIAMSPLSNNSLFLEYAKNPFLDFLQKGLMISLSTDDPMQFHFTKEPLMEEYAIAAQVFKLSTCDMCEVARNSVLQCGISHEEKAKFLGNNYLEEGPVGNDIRRTNVAQIRMAYRYETWCYELNLIAEGLKATE

Target ORF information:

RefSeq Version	XM_006501401.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501401.3

ORF Insert Sequence:

ATGAACCAGA AGCACCTGCT GCGCTTCATT AAGAAATCTT ACCATATTGA CGCCGACAGG 
GTGGTCTACA GCACTAAAGA GAAGAGCCTG ACTCTGAAGG AACTTTTCGC TAAGTTGAAT 
ATGCATCCCT ATGACCTGAC TGTTGACTCT CTGGATGTTC ATGCTGGACG CCAGACGTTC 
CAACGTTTTG ATAAGTTCAA TGACAAATAC AATCCCGTGG GTGCAAGTGA GCTTCGGGAC 
CTCTACCTAA AAACAGACAA CTACATTAAT GGGGAGTATT TTGCCACTAT CATCAAGGAG 
GTGGGTGCAG ACCTGGTGGA GGCCAAGTAT CAGCATGCAG AGCCTCGCTT ATCCATCTAC 
GGTCGCAGTC CAGATGAGTG GAACAAACTC TCCTCTTGGT TTGTCTGCAA TCGCATCTAT 
TGCCCCAACA TGACGTGGAT GATCCAGGTC CCCAGGATCT ATGATGTGTT CCGATCCAAG 
AATTTCCTGC CACACTTCGG AAAGATGCTG GAGAATATTT TCCTTCCAGT GTTTGAGGCC 
ACCATCAACC CCCAAGCTCA CCCAGACCTC AGTGTCTTCC TCAAGCACAT CACTGGCTTT 
GACAGTGTGG ATGATGAATC CAAACACAGT GGTCACATGT TTTCCTCCAA GAGTCCCAAA 
CCCGAGGAGT GGACAATGGA AAACAACCCG TCTTATACTT ACTATGCCTA CTACATGTAT 
GCAAACATCA CGGTGCTCAA CAGCCTGAGA AAGGAACGAG GCATGAATAC ATTTCTGTTT 
CGTCCCCACT GTGGGGAGGC TGGGGCTCTC ACTCACCTCA TGACAGCCTT CATGATAGCA 
GACAATATTT CTCATGGACT GAATTTAAAG AAGAGTCCTG TGCTACAGTA CTTGTTTTTC 
CTAGCCCAGA TTCCCATCGC CATGTCACCG CTGAGTAACA ACAGTCTGTT CCTCGAATAT 
GCAAAGAATC CTTTCTTAGA TTTCCTCCAG AAAGGCCTGA TGATCTCACT GTCGACCGAT 
GACCCGATGC AGTTCCACTT CACCAAGGAG CCCCTGATGG AAGAATACGC CATCGCTGCG 
CAAGTCTTCA AGCTGAGCAC GTGTGACATG TGTGAGGTGG CAAGGAACAG TGTCCTGCAG 
TGTGGGATTT CTCATGAGGA AAAAGCAAAG TTTCTGGGCA ACAATTACCT TGAGGAAGGC 
CCTGTTGGAA ATGACATCCG GAGGACAAAT GTCGCTCAGA TTCGCATGGC CTATCGTTAT 
GAAACTTGGT GTTATGAACT CAATTTAATT GCTGAGGGTC TTAAAGCAAC AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ampd1 ( NM_001033303.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028475.2 Mus musculus adenosine monophosphate deaminase 1 (Ampd1), mRNA.

Price & Availability↑

CloneID	OMu20693
Clone ID Related Accession (Same CDS sequence)	NM_001033303.2
Accession Version	NM_001033303.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	AMP deaminase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163297.4 and AC150894.4. On Sep 15, 2006 this sequence version replaced NM_001033303.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132524.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCTAT TCAAACTAAC AGGTCAAGGA AAACAAATTG ATGATGCAAT GCGTAGCTTT 
GCTGAAAAAG TGTTTGCCTC TGAAGTCAAA GATGAGGGAG GTCGGCACGA GATCTCCCCC 
TTCGACGTGG ATGAGATCTG CCCAATTTCC CTTCATGAGA TGCAGGCCCA CATATTCCAC 
ATGGAGAACC TGTCCATGGA TGGCAGGAGA AAAAGGCGTT TCCAAGGACG GAAGACTGTT 
AATTTGTCCA TTCCACAAAG TGAAACATCT TCTACCAAAC TGTCCCACAT CGAAGAATTT 
ATTTCTTCAT CCCCAACCTA CGAGAGTGTG CCTGACTTCC AGAGGGTGCA GATCACTGGA 
GACTATGCCT CGGGGGTAAC CGTTGAAGAC TTTGAGGTGG TTTGTAAAGG TCTCTATCGG 
GCATTGTGTA TACGAGAGAA ATACATGCAG AAGTCATTCC AGAGGTTCCC CAAGACCCCC 
TCCAAGTACT TGAGGAACAT TGATGGCGAA GCCTTGGTAG GAAACGAGAG CTTCTACCCA 
GTATTTACCC CTCCTCCGAA GAAGGGTGAA GACCCCTTTC GCACAGAGGA CCTTCCTGCA 
AACCTGGGCT ATCACCTCAA GATGAAAGCT GGTGTGATTT ACATCTACCC TGATGAAGCG 
GCAGCCAACA GAGATGAGCC CAAGCCCTAC CCTTACCCAA ATCTGGATGA CTTCCTGGAT 
GACATGAATT TTTTGCTTGC TCTAATTGCA CAAGGGCCTG TTAAGACTTA TGCTCACCGC 
CGCCTGAAGT TCCTCTCCTC CAAGTTCCAG GTCCATCAGA TGCTCAACGA GATGGATGAG 
CTGAAGGAGC TGAAGAACAA CCCCCACCGG GACTTTTATA ACTGCAGGAA GGTGGATACT 
CACATCCATG CAGCTGCCTG CATGAACCAG AAGCACCTGC TGCGCTTCAT TAAGAAATCT 
TACCATATTG ACGCCGACAG GGTGGTCTAC AGCACTAAAG AGAAGAGCCT GACTCTGAAG 
GAACTTTTCG CTAAGTTGAA TATGCATCCC TATGACCTGA CTGTTGACTC TCTGGATGTT 
CATGCTGGAC GCCAGACGTT CCAACGTTTT GATAAGTTCA ATGACAAATA CAATCCCGTG 
GGTGCAAGTG AGCTTCGGGA CCTCTACCTA AAAACAGACA ACTACATTAA TGGGGAGTAT 
TTTGCCACTA TCATCAAGGA GGTGGGTGCA GACCTGGTGG AGGCCAAGTA TCAGCATGCA 
GAGCCTCGCT TATCCATCTA CGGTCGCAGT CCAGATGAGT GGAACAAACT CTCCTCTTGG 
TTTGTCTGCA ATCGCATCTA TTGCCCCAAC ATGACGTGGA TGATCCAGGT CCCCAGGATC 
TATGATGTGT TCCGATCCAA GAATTTCCTG CCACACTTCG GAAAGATGCT GGAGAATATT 
TTCCTTCCAG TGTTTGAGGC CACCATCAAC CCCCAAGCTC ACCCAGACCT CAGTGTCTTC 
CTCAAGCACA TCACTGGCTT TGACAGTGTG GATGATGAAT CCAAACACAG TGGTCACATG 
TTTTCCTCCA AGAGTCCCAA ACCCGAGGAG TGGACAATGG AAAACAACCC GTCTTATACT 
TACTATGCCT ACTACATGTA TGCAAACATC ACGGTGCTCA ACAGCCTGAG AAAGGAACGA 
GGCATGAATA CATTTCTGTT TCGTCCCCAC TGTGGGGAGG CTGGGGCTCT CACTCACCTC 
ATGACAGCCT TCATGATAGC AGACAATATT TCTCATGGAC TGAATTTAAA GAAGAGTCCT 
GTGCTACAGT ACTTGTTTTT CCTAGCCCAG ATTCCCATCG CCATGTCACC GCTGAGTAAC 
AACAGTCTGT TCCTCGAATA TGCAAAGAAT CCTTTCTTAG ATTTCCTCCA GAAAGGCCTG 
ATGATCTCAC TGTCGACCGA TGACCCGATG CAGTTCCACT TCACCAAGGA GCCCCTGATG 
GAAGAATACG CCATCGCTGC GCAAGTCTTC AAGCTGAGCA CGTGTGACAT GTGTGAGGTG 
GCAAGGAACA GTGTCCTGCA GTGTGGGATT TCTCATGAGG AAAAAGCAAA GTTTCTGGGC 
AACAATTACC TTGAGGAAGG CCCTGTTGGA AATGACATCC GGAGGACAAA TGTCGCTCAG 
ATTCGCATGG CCTATCGTTA TGAAACTTGG TGTTATGAAC TCAATTTAAT TGCTGAGGGT 
CTTAAAGCAA CAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028475.2
CDS	83..2320
Translation	MPLFKLTGQGKQIDDAMRSFAEKVFASEVKDEGGRHEISPFDVDEICPISLHEMQAHIFHMENLSMDGRRKRRFQGRKTVNLSIPQSETSSTKLSHIEEFISSSPTYESVPDFQRVQITGDYASGVTVEDFEVVCKGLYRALCIREKYMQKSFQRFPKTPSKYLRNIDGEALVGNESFYPVFTPPPKKGEDPFRTEDLPANLGYHLKMKAGVIYIYPDEAAANRDEPKPYPYPNLDDFLDDMNFLLALIAQGPVKTYAHRRLKFLSSKFQVHQMLNEMDELKELKNNPHRDFYNCRKVDTHIHAAACMNQKHLLRFIKKSYHIDADRVVYSTKEKSLTLKELFAKLNMHPYDLTVDSLDVHAGRQTFQRFDKFNDKYNPVGASELRDLYLKTDNYINGEYFATIIKEVGADLVEAKYQHAEPRLSIYGRSPDEWNKLSSWFVCNRIYCPNMTWMIQVPRIYDVFRSKNFLPHFGKMLENIFLPVFEATINPQAHPDLSVFLKHITGFDSVDDESKHSGHMFSSKSPKPEEWTMENNPSYTYYAYYMYANITVLNSLRKERGMNTFLFRPHCGEAGALTHLMTAFMIADNISHGLNLKKSPVLQYLFFLAQIPIAMSPLSNNSLFLEYAKNPFLDFLQKGLMISLSTDDPMQFHFTKEPLMEEYAIAAQVFKLSTCDMCEVARNSVLQCGISHEEKAKFLGNNYLEEGPVGNDIRRTNVAQIRMAYRYETWCYELNLIAEGLKATE

Target ORF information:

RefSeq Version	NM_001033303.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenosine monophosphate deaminase 1 (Ampd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033303.2

ORF Insert Sequence:

ATGCCTCTAT TCAAACTAAC AGGTCAAGGA AAACAAATTG ATGATGCAAT GCGTAGCTTT 
GCTGAAAAAG TGTTTGCCTC TGAAGTCAAA GATGAGGGAG GTCGGCACGA GATCTCCCCC 
TTCGACGTGG ATGAGATCTG CCCAATTTCC CTTCATGAGA TGCAGGCCCA CATATTCCAC 
ATGGAGAACC TGTCCATGGA TGGCAGGAGA AAAAGGCGTT TCCAAGGACG GAAGACTGTT 
AATTTGTCCA TTCCACAAAG TGAAACATCT TCTACCAAAC TGTCCCACAT CGAAGAATTT 
ATTTCTTCAT CCCCAACCTA CGAGAGTGTG CCTGACTTCC AGAGGGTGCA GATCACTGGA 
GACTATGCCT CGGGGGTAAC CGTTGAAGAC TTTGAGGTGG TTTGTAAAGG TCTCTATCGG 
GCATTGTGTA TACGAGAGAA ATACATGCAG AAGTCATTCC AGAGGTTCCC CAAGACCCCC 
TCCAAGTACT TGAGGAACAT TGATGGCGAA GCCTTGGTAG GAAACGAGAG CTTCTACCCA 
GTATTTACCC CTCCTCCGAA GAAGGGTGAA GACCCCTTTC GCACAGAGGA CCTTCCTGCA 
AACCTGGGCT ATCACCTCAA GATGAAAGCT GGTGTGATTT ACATCTACCC TGATGAAGCG 
GCAGCCAACA GAGATGAGCC CAAGCCCTAC CCTTACCCAA ATCTGGATGA CTTCCTGGAT 
GACATGAATT TTTTGCTTGC TCTAATTGCA CAAGGGCCTG TTAAGACTTA TGCTCACCGC 
CGCCTGAAGT TCCTCTCCTC CAAGTTCCAG GTCCATCAGA TGCTCAACGA GATGGATGAG 
CTGAAGGAGC TGAAGAACAA CCCCCACCGG GACTTTTATA ACTGCAGGAA GGTGGATACT 
CACATCCATG CAGCTGCCTG CATGAACCAG AAGCACCTGC TGCGCTTCAT TAAGAAATCT 
TACCATATTG ACGCCGACAG GGTGGTCTAC AGCACTAAAG AGAAGAGCCT GACTCTGAAG 
GAACTTTTCG CTAAGTTGAA TATGCATCCC TATGACCTGA CTGTTGACTC TCTGGATGTT 
CATGCTGGAC GCCAGACGTT CCAACGTTTT GATAAGTTCA ATGACAAATA CAATCCCGTG 
GGTGCAAGTG AGCTTCGGGA CCTCTACCTA AAAACAGACA ACTACATTAA TGGGGAGTAT 
TTTGCCACTA TCATCAAGGA GGTGGGTGCA GACCTGGTGG AGGCCAAGTA TCAGCATGCA 
GAGCCTCGCT TATCCATCTA CGGTCGCAGT CCAGATGAGT GGAACAAACT CTCCTCTTGG 
TTTGTCTGCA ATCGCATCTA TTGCCCCAAC ATGACGTGGA TGATCCAGGT CCCCAGGATC 
TATGATGTGT TCCGATCCAA GAATTTCCTG CCACACTTCG GAAAGATGCT GGAGAATATT 
TTCCTTCCAG TGTTTGAGGC CACCATCAAC CCCCAAGCTC ACCCAGACCT CAGTGTCTTC 
CTCAAGCACA TCACTGGCTT TGACAGTGTG GATGATGAAT CCAAACACAG TGGTCACATG 
TTTTCCTCCA AGAGTCCCAA ACCCGAGGAG TGGACAATGG AAAACAACCC GTCTTATACT 
TACTATGCCT ACTACATGTA TGCAAACATC ACGGTGCTCA ACAGCCTGAG AAAGGAACGA 
GGCATGAATA CATTTCTGTT TCGTCCCCAC TGTGGGGAGG CTGGGGCTCT CACTCACCTC 
ATGACAGCCT TCATGATAGC AGACAATATT TCTCATGGAC TGAATTTAAA GAAGAGTCCT 
GTGCTACAGT ACTTGTTTTT CCTAGCCCAG ATTCCCATCG CCATGTCACC GCTGAGTAAC 
AACAGTCTGT TCCTCGAATA TGCAAAGAAT CCTTTCTTAG ATTTCCTCCA GAAAGGCCTG 
ATGATCTCAC TGTCGACCGA TGACCCGATG CAGTTCCACT TCACCAAGGA GCCCCTGATG 
GAAGAATACG CCATCGCTGC GCAAGTCTTC AAGCTGAGCA CGTGTGACAT GTGTGAGGTG 
GCAAGGAACA GTGTCCTGCA GTGTGGGATT TCTCATGAGG AAAAAGCAAA GTTTCTGGGC 
AACAATTACC TTGAGGAAGG CCCTGTTGGA AATGACATCC GGAGGACAAA TGTCGCTCAG 
ATTCGCATGG CCTATCGTTA TGAAACTTGG TGTTATGAAC TCAATTTAAT TGCTGAGGGT 
CTTAAAGCAA CAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ampd1 ( XM_006501400.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501463.1 Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31857
Clone ID Related Accession (Same CDS sequence)	XM_006501400.3 , XM_006501400.4
Accession Version	XM_006501400.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	AMP deaminase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501400.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTCC TGGTATTTCA GAGAGTCATT CCGCCATCCC CACAGTCTCT CTCCTTCCTC 
TGCTGTGCTA TCCTAGAATC AAGGATCCCA GCCACAATGC CTCTATTCAA ACTAACAGAA 
ATTGATGATG CAATGCGTAG CTTTGCTGAA AAAGTGTTTG CCTCTGAAGT CAAAGATGAG 
GGAGGTCGGC ACGAGATCTC CCCCTTCGAC GTGGATGAGA TCTGCCCAAT TTCCCTTCAT 
GAGATGCAGG CCCACATATT CCACATGGAG AACCTGTCCA TGGATGGCAG GAGAAAAAGG 
CGTTTCCAAG GACGGAAGAC TGTTAATTTG TCCATTCCAC AAAGTGAAAC ATCTTCTACC 
AAACTGTCCC ACATCGAAGA ATTTATTTCT TCATCCCCAA CCTACGAGAG TGTGCCTGAC 
TTCCAGAGGG TGCAGATCAC TGGAGACTAT GCCTCGGGGG TAACCGTTGA AGACTTTGAG 
GTGGTTTGTA AAGGTCTCTA TCGGGCATTG TGTATACGAG AGAAATACAT GCAGAAGTCA 
TTCCAGAGGT TCCCCAAGAC CCCCTCCAAG TACTTGAGGA ACATTGATGG CGAAGCCTTG 
GTAGGAAACG AGAGCTTCTA CCCAGTATTT ACCCCTCCTC CGAAGAAGGG TGAAGACCCC 
TTTCGCACAG AGGACCTTCC TGCAAACCTG GGCTATCACC TCAAGATGAA AGCTGGTGTG 
ATTTACATCT ACCCTGATGA AGCGGCAGCC AACAGAGATG AGCCCAAGCC CTACCCTTAC 
CCAAATCTGG ATGACTTCCT GGATGACATG AATTTTTTGC TTGCTCTAAT TGCACAAGGG 
CCTGTTAAGA CTTATGCTCA CCGCCGCCTG AAGTTCCTCT CCTCCAAGTT CCAGGTCCAT 
CAGATGCTCA ACGAGATGGA TGAGCTGAAG GAGCTGAAGA ACAACCCCCA CCGGGACTTT 
TATAACTGCA GGAAGGTGGA TACTCACATC CATGCAGCTG CCTGCATGAA CCAGAAGCAC 
CTGCTGCGCT TCATTAAGAA ATCTTACCAT ATTGACGCCG ACAGGGTGGT CTACAGCACT 
AAAGAGAAGA GCCTGACTCT GAAGGAACTT TTCGCTAAGT TGAATATGCA TCCCTATGAC 
CTGACTGTTG ACTCTCTGGA TGTTCATGCT GGACGCCAGA CGTTCCAACG TTTTGATAAG 
TTCAATGACA AATACAATCC CGTGGGTGCA AGTGAGCTTC GGGACCTCTA CCTAAAAACA 
GACAACTACA TTAATGGGGA GTATTTTGCC ACTATCATCA AGGAGGTGGG TGCAGACCTG 
GTGGAGGCCA AGTATCAGCA TGCAGAGCCT CGCTTATCCA TCTACGGTCG CAGTCCAGAT 
GAGTGGAACA AACTCTCCTC TTGGTTTGTC TGCAATCGCA TCTATTGCCC CAACATGACG 
TGGATGATCC AGGTCCCCAG GATCTATGAT GTGTTCCGAT CCAAGAATTT CCTGCCACAC 
TTCGGAAAGA TGCTGGAGAA TATTTTCCTT CCAGTGTTTG AGGCCACCAT CAACCCCCAA 
GCTCACCCAG ACCTCAGTGT CTTCCTCAAG CACATCACTG GCTTTGACAG TGTGGATGAT 
GAATCCAAAC ACAGTGGTCA CATGTTTTCC TCCAAGAGTC CCAAACCCGA GGAGTGGACA 
ATGGAAAACA ACCCGTCTTA TACTTACTAT GCCTACTACA TGTATGCAAA CATCACGGTG 
CTCAACAGCC TGAGAAAGGA ACGAGGCATG AATACATTTC TGTTTCGTCC CCACTGTGGG 
GAGGCTGGGG CTCTCACTCA CCTCATGACA GCCTTCATGA TAGCAGACAA TATTTCTCAT 
GGACTGAATT TAAAGAAGAG TCCTGTGCTA CAGTACTTGT TTTTCCTAGC CCAGATTCCC 
ATCGCCATGT CACCGCTGAG TAACAACAGT CTGTTCCTCG AATATGCAAA GAATCCTTTC 
TTAGATTTCC TCCAGAAAGG CCTGATGATC TCACTGTCGA CCGATGACCC GATGCAGTTC 
CACTTCACCA AGGAGCCCCT GATGGAAGAA TACGCCATCG CTGCGCAAGT CTTCAAGCTG 
AGCACGTGTG ACATGTGTGA GGTGGCAAGG AACAGTGTCC TGCAGTGTGG GATTTCTCAT 
GAGGAAAAAG CAAAGTTTCT GGGCAACAAT TACCTTGAGG AAGGCCCTGT TGGAAATGAC 
ATCCGGAGGA CAAATGTCGC TCAGATTCGC ATGGCCTATC GTTATGAAAC TTGGTGTTAT 
GAACTCAATT TAATTGCTGA GGGTCTTAAA GCAACAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501463.1
CDS	433..2754
Translation	MSVLVFQRVIPPSPQSLSFLCCAILESRIPATMPLFKLTEIDDAMRSFAEKVFASEVKDEGGRHEISPFDVDEICPISLHEMQAHIFHMENLSMDGRRKRRFQGRKTVNLSIPQSETSSTKLSHIEEFISSSPTYESVPDFQRVQITGDYASGVTVEDFEVVCKGLYRALCIREKYMQKSFQRFPKTPSKYLRNIDGEALVGNESFYPVFTPPPKKGEDPFRTEDLPANLGYHLKMKAGVIYIYPDEAAANRDEPKPYPYPNLDDFLDDMNFLLALIAQGPVKTYAHRRLKFLSSKFQVHQMLNEMDELKELKNNPHRDFYNCRKVDTHIHAAACMNQKHLLRFIKKSYHIDADRVVYSTKEKSLTLKELFAKLNMHPYDLTVDSLDVHAGRQTFQRFDKFNDKYNPVGASELRDLYLKTDNYINGEYFATIIKEVGADLVEAKYQHAEPRLSIYGRSPDEWNKLSSWFVCNRIYCPNMTWMIQVPRIYDVFRSKNFLPHFGKMLENIFLPVFEATINPQAHPDLSVFLKHITGFDSVDDESKHSGHMFSSKSPKPEEWTMENNPSYTYYAYYMYANITVLNSLRKERGMNTFLFRPHCGEAGALTHLMTAFMIADNISHGLNLKKSPVLQYLFFLAQIPIAMSPLSNNSLFLEYAKNPFLDFLQKGLMISLSTDDPMQFHFTKEPLMEEYAIAAQVFKLSTCDMCEVARNSVLQCGISHEEKAKFLGNNYLEEGPVGNDIRRTNVAQIRMAYRYETWCYELNLIAEGLKATE

Target ORF information:

RefSeq Version	XM_006501400.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501400.3

ORF Insert Sequence:

ATGAGTGTCC TGGTATTTCA GAGAGTCATT CCGCCATCCC CACAGTCTCT CTCCTTCCTC 
TGCTGTGCTA TCCTAGAATC AAGGATCCCA GCCACAATGC CTCTATTCAA ACTAACAGAA 
ATTGATGATG CAATGCGTAG CTTTGCTGAA AAAGTGTTTG CCTCTGAAGT CAAAGATGAG 
GGAGGTCGGC ACGAGATCTC CCCCTTCGAC GTGGATGAGA TCTGCCCAAT TTCCCTTCAT 
GAGATGCAGG CCCACATATT CCACATGGAG AACCTGTCCA TGGATGGCAG GAGAAAAAGG 
CGTTTCCAAG GACGGAAGAC TGTTAATTTG TCCATTCCAC AAAGTGAAAC ATCTTCTACC 
AAACTGTCCC ACATCGAAGA ATTTATTTCT TCATCCCCAA CCTACGAGAG TGTGCCTGAC 
TTCCAGAGGG TGCAGATCAC TGGAGACTAT GCCTCGGGGG TAACCGTTGA AGACTTTGAG 
GTGGTTTGTA AAGGTCTCTA TCGGGCATTG TGTATACGAG AGAAATACAT GCAGAAGTCA 
TTCCAGAGGT TCCCCAAGAC CCCCTCCAAG TACTTGAGGA ACATTGATGG CGAAGCCTTG 
GTAGGAAACG AGAGCTTCTA CCCAGTATTT ACCCCTCCTC CGAAGAAGGG TGAAGACCCC 
TTTCGCACAG AGGACCTTCC TGCAAACCTG GGCTATCACC TCAAGATGAA AGCTGGTGTG 
ATTTACATCT ACCCTGATGA AGCGGCAGCC AACAGAGATG AGCCCAAGCC CTACCCTTAC 
CCAAATCTGG ATGACTTCCT GGATGACATG AATTTTTTGC TTGCTCTAAT TGCACAAGGG 
CCTGTTAAGA CTTATGCTCA CCGCCGCCTG AAGTTCCTCT CCTCCAAGTT CCAGGTCCAT 
CAGATGCTCA ACGAGATGGA TGAGCTGAAG GAGCTGAAGA ACAACCCCCA CCGGGACTTT 
TATAACTGCA GGAAGGTGGA TACTCACATC CATGCAGCTG CCTGCATGAA CCAGAAGCAC 
CTGCTGCGCT TCATTAAGAA ATCTTACCAT ATTGACGCCG ACAGGGTGGT CTACAGCACT 
AAAGAGAAGA GCCTGACTCT GAAGGAACTT TTCGCTAAGT TGAATATGCA TCCCTATGAC 
CTGACTGTTG ACTCTCTGGA TGTTCATGCT GGACGCCAGA CGTTCCAACG TTTTGATAAG 
TTCAATGACA AATACAATCC CGTGGGTGCA AGTGAGCTTC GGGACCTCTA CCTAAAAACA 
GACAACTACA TTAATGGGGA GTATTTTGCC ACTATCATCA AGGAGGTGGG TGCAGACCTG 
GTGGAGGCCA AGTATCAGCA TGCAGAGCCT CGCTTATCCA TCTACGGTCG CAGTCCAGAT 
GAGTGGAACA AACTCTCCTC TTGGTTTGTC TGCAATCGCA TCTATTGCCC CAACATGACG 
TGGATGATCC AGGTCCCCAG GATCTATGAT GTGTTCCGAT CCAAGAATTT CCTGCCACAC 
TTCGGAAAGA TGCTGGAGAA TATTTTCCTT CCAGTGTTTG AGGCCACCAT CAACCCCCAA 
GCTCACCCAG ACCTCAGTGT CTTCCTCAAG CACATCACTG GCTTTGACAG TGTGGATGAT 
GAATCCAAAC ACAGTGGTCA CATGTTTTCC TCCAAGAGTC CCAAACCCGA GGAGTGGACA 
ATGGAAAACA ACCCGTCTTA TACTTACTAT GCCTACTACA TGTATGCAAA CATCACGGTG 
CTCAACAGCC TGAGAAAGGA ACGAGGCATG AATACATTTC TGTTTCGTCC CCACTGTGGG 
GAGGCTGGGG CTCTCACTCA CCTCATGACA GCCTTCATGA TAGCAGACAA TATTTCTCAT 
GGACTGAATT TAAAGAAGAG TCCTGTGCTA CAGTACTTGT TTTTCCTAGC CCAGATTCCC 
ATCGCCATGT CACCGCTGAG TAACAACAGT CTGTTCCTCG AATATGCAAA GAATCCTTTC 
TTAGATTTCC TCCAGAAAGG CCTGATGATC TCACTGTCGA CCGATGACCC GATGCAGTTC 
CACTTCACCA AGGAGCCCCT GATGGAAGAA TACGCCATCG CTGCGCAAGT CTTCAAGCTG 
AGCACGTGTG ACATGTGTGA GGTGGCAAGG AACAGTGTCC TGCAGTGTGG GATTTCTCAT 
GAGGAAAAAG CAAAGTTTCT GGGCAACAAT TACCTTGAGG AAGGCCCTGT TGGAAATGAC 
ATCCGGAGGA CAAATGTCGC TCAGATTCGC ATGGCCTATC GTTATGAAAC TTGGTGTTAT 
GAACTCAATT TAATTGCTGA GGGTCTTAAA GCAACAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ampd1 ( XM_006501400.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501463.1 Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31857
Clone ID Related Accession (Same CDS sequence)	XM_006501400.3 , XM_006501400.4
Accession Version	XM_006501400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AMP deaminase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501400.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTCC TGGTATTTCA GAGAGTCATT CCGCCATCCC CACAGTCTCT CTCCTTCCTC 
TGCTGTGCTA TCCTAGAATC AAGGATCCCA GCCACAATGC CTCTATTCAA ACTAACAGAA 
ATTGATGATG CAATGCGTAG CTTTGCTGAA AAAGTGTTTG CCTCTGAAGT CAAAGATGAG 
GGAGGTCGGC ACGAGATCTC CCCCTTCGAC GTGGATGAGA TCTGCCCAAT TTCCCTTCAT 
GAGATGCAGG CCCACATATT CCACATGGAG AACCTGTCCA TGGATGGCAG GAGAAAAAGG 
CGTTTCCAAG GACGGAAGAC TGTTAATTTG TCCATTCCAC AAAGTGAAAC ATCTTCTACC 
AAACTGTCCC ACATCGAAGA ATTTATTTCT TCATCCCCAA CCTACGAGAG TGTGCCTGAC 
TTCCAGAGGG TGCAGATCAC TGGAGACTAT GCCTCGGGGG TAACCGTTGA AGACTTTGAG 
GTGGTTTGTA AAGGTCTCTA TCGGGCATTG TGTATACGAG AGAAATACAT GCAGAAGTCA 
TTCCAGAGGT TCCCCAAGAC CCCCTCCAAG TACTTGAGGA ACATTGATGG CGAAGCCTTG 
GTAGGAAACG AGAGCTTCTA CCCAGTATTT ACCCCTCCTC CGAAGAAGGG TGAAGACCCC 
TTTCGCACAG AGGACCTTCC TGCAAACCTG GGCTATCACC TCAAGATGAA AGCTGGTGTG 
ATTTACATCT ACCCTGATGA AGCGGCAGCC AACAGAGATG AGCCCAAGCC CTACCCTTAC 
CCAAATCTGG ATGACTTCCT GGATGACATG AATTTTTTGC TTGCTCTAAT TGCACAAGGG 
CCTGTTAAGA CTTATGCTCA CCGCCGCCTG AAGTTCCTCT CCTCCAAGTT CCAGGTCCAT 
CAGATGCTCA ACGAGATGGA TGAGCTGAAG GAGCTGAAGA ACAACCCCCA CCGGGACTTT 
TATAACTGCA GGAAGGTGGA TACTCACATC CATGCAGCTG CCTGCATGAA CCAGAAGCAC 
CTGCTGCGCT TCATTAAGAA ATCTTACCAT ATTGACGCCG ACAGGGTGGT CTACAGCACT 
AAAGAGAAGA GCCTGACTCT GAAGGAACTT TTCGCTAAGT TGAATATGCA TCCCTATGAC 
CTGACTGTTG ACTCTCTGGA TGTTCATGCT GGACGCCAGA CGTTCCAACG TTTTGATAAG 
TTCAATGACA AATACAATCC CGTGGGTGCA AGTGAGCTTC GGGACCTCTA CCTAAAAACA 
GACAACTACA TTAATGGGGA GTATTTTGCC ACTATCATCA AGGAGGTGGG TGCAGACCTG 
GTGGAGGCCA AGTATCAGCA TGCAGAGCCT CGCTTATCCA TCTACGGTCG CAGTCCAGAT 
GAGTGGAACA AACTCTCCTC TTGGTTTGTC TGCAATCGCA TCTATTGCCC CAACATGACG 
TGGATGATCC AGGTCCCCAG GATCTATGAT GTGTTCCGAT CCAAGAATTT CCTGCCACAC 
TTCGGAAAGA TGCTGGAGAA TATTTTCCTT CCAGTGTTTG AGGCCACCAT CAACCCCCAA 
GCTCACCCAG ACCTCAGTGT CTTCCTCAAG CACATCACTG GCTTTGACAG TGTGGATGAT 
GAATCCAAAC ACAGTGGTCA CATGTTTTCC TCCAAGAGTC CCAAACCCGA GGAGTGGACA 
ATGGAAAACA ACCCGTCTTA TACTTACTAT GCCTACTACA TGTATGCAAA CATCACGGTG 
CTCAACAGCC TGAGAAAGGA ACGAGGCATG AATACATTTC TGTTTCGTCC CCACTGTGGG 
GAGGCTGGGG CTCTCACTCA CCTCATGACA GCCTTCATGA TAGCAGACAA TATTTCTCAT 
GGACTGAATT TAAAGAAGAG TCCTGTGCTA CAGTACTTGT TTTTCCTAGC CCAGATTCCC 
ATCGCCATGT CACCGCTGAG TAACAACAGT CTGTTCCTCG AATATGCAAA GAATCCTTTC 
TTAGATTTCC TCCAGAAAGG CCTGATGATC TCACTGTCGA CCGATGACCC GATGCAGTTC 
CACTTCACCA AGGAGCCCCT GATGGAAGAA TACGCCATCG CTGCGCAAGT CTTCAAGCTG 
AGCACGTGTG ACATGTGTGA GGTGGCAAGG AACAGTGTCC TGCAGTGTGG GATTTCTCAT 
GAGGAAAAAG CAAAGTTTCT GGGCAACAAT TACCTTGAGG AAGGCCCTGT TGGAAATGAC 
ATCCGGAGGA CAAATGTCGC TCAGATTCGC ATGGCCTATC GTTATGAAAC TTGGTGTTAT 
GAACTCAATT TAATTGCTGA GGGTCTTAAA GCAACAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501463.1
CDS	104..2425
Translation	MSVLVFQRVIPPSPQSLSFLCCAILESRIPATMPLFKLTEIDDAMRSFAEKVFASEVKDEGGRHEISPFDVDEICPISLHEMQAHIFHMENLSMDGRRKRRFQGRKTVNLSIPQSETSSTKLSHIEEFISSSPTYESVPDFQRVQITGDYASGVTVEDFEVVCKGLYRALCIREKYMQKSFQRFPKTPSKYLRNIDGEALVGNESFYPVFTPPPKKGEDPFRTEDLPANLGYHLKMKAGVIYIYPDEAAANRDEPKPYPYPNLDDFLDDMNFLLALIAQGPVKTYAHRRLKFLSSKFQVHQMLNEMDELKELKNNPHRDFYNCRKVDTHIHAAACMNQKHLLRFIKKSYHIDADRVVYSTKEKSLTLKELFAKLNMHPYDLTVDSLDVHAGRQTFQRFDKFNDKYNPVGASELRDLYLKTDNYINGEYFATIIKEVGADLVEAKYQHAEPRLSIYGRSPDEWNKLSSWFVCNRIYCPNMTWMIQVPRIYDVFRSKNFLPHFGKMLENIFLPVFEATINPQAHPDLSVFLKHITGFDSVDDESKHSGHMFSSKSPKPEEWTMENNPSYTYYAYYMYANITVLNSLRKERGMNTFLFRPHCGEAGALTHLMTAFMIADNISHGLNLKKSPVLQYLFFLAQIPIAMSPLSNNSLFLEYAKNPFLDFLQKGLMISLSTDDPMQFHFTKEPLMEEYAIAAQVFKLSTCDMCEVARNSVLQCGISHEEKAKFLGNNYLEEGPVGNDIRRTNVAQIRMAYRYETWCYELNLIAEGLKATE

Target ORF information:

RefSeq Version	XM_006501400.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenosine monophosphate deaminase 1 (Ampd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501400.4

ORF Insert Sequence:

ATGAGTGTCC TGGTATTTCA GAGAGTCATT CCGCCATCCC CACAGTCTCT CTCCTTCCTC 
TGCTGTGCTA TCCTAGAATC AAGGATCCCA GCCACAATGC CTCTATTCAA ACTAACAGAA 
ATTGATGATG CAATGCGTAG CTTTGCTGAA AAAGTGTTTG CCTCTGAAGT CAAAGATGAG 
GGAGGTCGGC ACGAGATCTC CCCCTTCGAC GTGGATGAGA TCTGCCCAAT TTCCCTTCAT 
GAGATGCAGG CCCACATATT CCACATGGAG AACCTGTCCA TGGATGGCAG GAGAAAAAGG 
CGTTTCCAAG GACGGAAGAC TGTTAATTTG TCCATTCCAC AAAGTGAAAC ATCTTCTACC 
AAACTGTCCC ACATCGAAGA ATTTATTTCT TCATCCCCAA CCTACGAGAG TGTGCCTGAC 
TTCCAGAGGG TGCAGATCAC TGGAGACTAT GCCTCGGGGG TAACCGTTGA AGACTTTGAG 
GTGGTTTGTA AAGGTCTCTA TCGGGCATTG TGTATACGAG AGAAATACAT GCAGAAGTCA 
TTCCAGAGGT TCCCCAAGAC CCCCTCCAAG TACTTGAGGA ACATTGATGG CGAAGCCTTG 
GTAGGAAACG AGAGCTTCTA CCCAGTATTT ACCCCTCCTC CGAAGAAGGG TGAAGACCCC 
TTTCGCACAG AGGACCTTCC TGCAAACCTG GGCTATCACC TCAAGATGAA AGCTGGTGTG 
ATTTACATCT ACCCTGATGA AGCGGCAGCC AACAGAGATG AGCCCAAGCC CTACCCTTAC 
CCAAATCTGG ATGACTTCCT GGATGACATG AATTTTTTGC TTGCTCTAAT TGCACAAGGG 
CCTGTTAAGA CTTATGCTCA CCGCCGCCTG AAGTTCCTCT CCTCCAAGTT CCAGGTCCAT 
CAGATGCTCA ACGAGATGGA TGAGCTGAAG GAGCTGAAGA ACAACCCCCA CCGGGACTTT 
TATAACTGCA GGAAGGTGGA TACTCACATC CATGCAGCTG CCTGCATGAA CCAGAAGCAC 
CTGCTGCGCT TCATTAAGAA ATCTTACCAT ATTGACGCCG ACAGGGTGGT CTACAGCACT 
AAAGAGAAGA GCCTGACTCT GAAGGAACTT TTCGCTAAGT TGAATATGCA TCCCTATGAC 
CTGACTGTTG ACTCTCTGGA TGTTCATGCT GGACGCCAGA CGTTCCAACG TTTTGATAAG 
TTCAATGACA AATACAATCC CGTGGGTGCA AGTGAGCTTC GGGACCTCTA CCTAAAAACA 
GACAACTACA TTAATGGGGA GTATTTTGCC ACTATCATCA AGGAGGTGGG TGCAGACCTG 
GTGGAGGCCA AGTATCAGCA TGCAGAGCCT CGCTTATCCA TCTACGGTCG CAGTCCAGAT 
GAGTGGAACA AACTCTCCTC TTGGTTTGTC TGCAATCGCA TCTATTGCCC CAACATGACG 
TGGATGATCC AGGTCCCCAG GATCTATGAT GTGTTCCGAT CCAAGAATTT CCTGCCACAC 
TTCGGAAAGA TGCTGGAGAA TATTTTCCTT CCAGTGTTTG AGGCCACCAT CAACCCCCAA 
GCTCACCCAG ACCTCAGTGT CTTCCTCAAG CACATCACTG GCTTTGACAG TGTGGATGAT 
GAATCCAAAC ACAGTGGTCA CATGTTTTCC TCCAAGAGTC CCAAACCCGA GGAGTGGACA 
ATGGAAAACA ACCCGTCTTA TACTTACTAT GCCTACTACA TGTATGCAAA CATCACGGTG 
CTCAACAGCC TGAGAAAGGA ACGAGGCATG AATACATTTC TGTTTCGTCC CCACTGTGGG 
GAGGCTGGGG CTCTCACTCA CCTCATGACA GCCTTCATGA TAGCAGACAA TATTTCTCAT 
GGACTGAATT TAAAGAAGAG TCCTGTGCTA CAGTACTTGT TTTTCCTAGC CCAGATTCCC 
ATCGCCATGT CACCGCTGAG TAACAACAGT CTGTTCCTCG AATATGCAAA GAATCCTTTC 
TTAGATTTCC TCCAGAAAGG CCTGATGATC TCACTGTCGA CCGATGACCC GATGCAGTTC 
CACTTCACCA AGGAGCCCCT GATGGAAGAA TACGCCATCG CTGCGCAAGT CTTCAAGCTG 
AGCACGTGTG ACATGTGTGA GGTGGCAAGG AACAGTGTCC TGCAGTGTGG GATTTCTCAT 
GAGGAAAAAG CAAAGTTTCT GGGCAACAAT TACCTTGAGG AAGGCCCTGT TGGAAATGAC 
ATCCGGAGGA CAAATGTCGC TCAGATTCGC ATGGCCTATC GTTATGAAAC TTGGTGTTAT 
GAACTCAATT TAATTGCTGA GGGTCTTAAA GCAACAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insertion of a knockout-first cassette in Ampd1 gene leads to neonatal death by disruption of neighboring genes expression.
Scientific reports635970(2016 Oct)
Pan Y,Zhang L,Liu Q,Li Y,Guo H,Peng Y,Peng H,Tang B,Hu Z,Zhao J,Xia K,Li JD

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

AMPD1 regulates mTORC1-p70 S6 kinase axis in the control of insulin sensitivity in skeletal muscle.
BMC endocrine disorders1511(2015 Mar)
Tandelilin AA,Hirase T,Hudoyo AW,Cheng J,Toyama K,Morisaki H,Morisaki T

AMPD1: a novel therapeutic target for reversing insulin resistance.
BMC endocrine disorders1496(2014 Dec)
Cheng J,Morisaki H,Toyama K,Sugimoto N,Shintani T,Tandelilin A,Hirase T,Holmes EW,Morisaki T

Effect of isolated AMP deaminase deficiency on skeletal muscle function.
Molecular genetics and metabolism reports151-59(2014)
Cheng J,Morisaki H,Sugimoto N,Dohi A,Shintani T,Kimura E,Toyama K,Ikawa M,Okabe M,Higuchi I,Matsuo S,Kawai Y,Hisatome I,Sugama T,Holmes EW,Morisaki T

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Ampd1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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