Eny2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eny2
Entrez Gene ID	223527
Full Name	ENY2 transcription and export complex 2 subunit
Synonyms	1810057B09Rik,6720481I12,DC6,Ey2
General protein information	Preferred Names ENY2 transcription and export complex 2 subunit Names transcription and mRNA export factor ENY2 e(y)2 homolog e(y)2 protein enhancer of yellow 2 homolog enhancer of yellow 2 transcription factor homolog enhancer of yellow eye homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 B3.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_175009.3	NP_778174.1	transcription and mRNA export factor ENY2
XM_006520758.2	XP_006520821.1	transcription and mRNA export factor ENY2 isoform X1
XM_006520758.3	XP_006520821.1	transcription and mRNA export factor ENY2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ENY2	XP_532308.1
Danio rerio (zebrafish)	eny2	NP_001002427.1
Homo sapiens (human)	ENY2	NP_064574.1
Arabidopsis thaliana (thale cress)	AT3G27100	NP_189346.2
	ENY2	XP_001087516.2
Oryza sativa (rice)	Os01g0920200	NP_001045218.1
Bos taurus (cattle)	ENY2	NP_001029919.1
Gallus gallus (chicken)	ENY2	NP_001264362.1
Pan troglodytes (chimpanzee)	ENY2	XP_003311939.1
Mus musculus (house mouse)	Eny2	NP_778174.1
Rattus norvegicus (Norway rat)	Eny2	NP_001124052.1
Xenopus tropicalis (tropical clawed frog)	eny2	NP_001123672.1

Gene Ontology
Bibliography

Related articles in PubMed

Suppression of the nuclear factor Eny2 increases insulin secretion in poorly functioning INS-1E insulinoma cells.
Dames P, Weise M, Puff R, G?ke B, Parhofer KG, Seissler J, Lechner A
Experimental diabetes research2012460869(2012)

Evolutionarily conserved E(y)2/Sus1 protein is essential for the barrier activity of Su(Hw)-dependent insulators in Drosophila.
Kurshakova M, Maksimenko O, Golovnin A, Pulina M, Georgieva S, Georgiev P, Krasnov A
Molecular cell27(2)332-8(2007 Jul)

Analysis of gene expression in the developing mouse retina.
D?az E, Yang YH, Ferreira T, Loh KC, Okazaki Y, Hayashizaki Y, Tessier-Lavigne M, Speed TP, Ngai J
Proceedings of the National Academy of Sciences of the United States of America100(9)5491-6(2003 Apr)

The novel transcription factor e(y)2 interacts with TAF(II)40 and potentiates transcription activation on chromatin templates.
Georgieva S, Nabirochkina E, Dilworth FJ, Eickhoff H, Becker P, Tora L, Georgiev P, Soldatov A
Molecular and cellular biology21(15)5223-31(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

there is a link between the nuclear protein Eny2 and insulin secretion
Title: Suppression of the nuclear factor Eny2 increases insulin secretion in poorly functioning INS-1E insulinoma cells.

E(y)2/Sus1, is recruited to the Su(Hw) insulators via binding to the zinc-finger domain of Su(Hw).
Title: Evolutionarily conserved E(y)2/Sus1 protein is essential for the barrier activity of Su(Hw)-dependent insulators in Drosophila.

The following Eny2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eny2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04764	NM_175009.3 Latest version!	Mus musculus ENY2 transcription and export complex 2 subunit (Eny2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu51163	XM_006520758.3 Latest version!	Mus musculus ENY2 transcription and export complex 2 subunit (Eny2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu51163	XM_006520758.2	Mus musculus enhancer of yellow 2 homolog (Drosophila) (Eny2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eny2 ( NM_175009.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_778174.1 Mus musculus ENY2 transcription and export complex 2 subunit (Eny2), mRNA.

Price & Availability↑

CloneID	OMu04764
Clone ID Related Accession (Same CDS sequence)	NM_175009.3
Accession Version	NM_175009.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	transcription and mRNA export factor ENY2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC127312.4. On Nov 17, 2006 this sequence version replaced NM_175009.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK032957.1, SRR1660811.305253.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGTTA GCAAGATGAA CAAAGATGCG CAGATGAGAG CAGCGATTAA CCAAAAGTTA 
ATAGAAACTG GAGAAAGAGA ACGCCTCAAA GAGTTGCTGA GAGCTAAATT AATTGAGTGC 
GGCTGGAAGG ATCAGCTAAA GGCACACTGT AAAGAGGTAA TTAAAGAAAA AGGACTAGAA 
CACGTTACTG TTGATGACTT GGTGGCTGAA ATCACTCCAA AAGGCAGAGC CCTGGTACCT 
GACAGTGTAA AGAAGGAGCT CCTACAGAGA ATAAGAACAT TCCTTGCTCA GCATGCCAGC 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_778174.1
CDS	127..432
Translation	MVVSKMNKDAQMRAAINQKLIETGERERLKELLRAKLIECGWKDQLKAHCKEVIKEKGLEHVTVDDLVAEITPKGRALVPDSVKKELLQRIRTFLAQHASL

Target ORF information:

RefSeq Version	NM_175009.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ENY2 transcription and export complex 2 subunit (Eny2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175009.3

ORF Insert Sequence:

ATGGTGGTTA GCAAGATGAA CAAAGATGCG CAGATGAGAG CAGCGATTAA CCAAAAGTTA 
ATAGAAACTG GAGAAAGAGA ACGCCTCAAA GAGTTGCTGA GAGCTAAATT AATTGAGTGC 
GGCTGGAAGG ATCAGCTAAA GGCACACTGT AAAGAGGTAA TTAAAGAAAA AGGACTAGAA 
CACGTTACTG TTGATGACTT GGTGGCTGAA ATCACTCCAA AAGGCAGAGC CCTGGTACCT 
GACAGTGTAA AGAAGGAGCT CCTACAGAGA ATAAGAACAT TCCTTGCTCA GCATGCCAGC 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eny2 ( XM_006520758.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520821.1 Mus musculus enhancer of yellow 2 homolog (Drosophila) (Eny2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu51163
Clone ID Related Accession (Same CDS sequence)	XM_006520758.2 , XM_006520758.3
Accession Version	XM_006520758.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transcription and mRNA export factor ENY2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006520758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG ATGCGCAGAT GAGAGCAGCG ATTAACCAAA AGTTAATAGA AACTGGAGAA 
AGAGAACGCC TCAAAGAGTT GCTGAGAGCT AAATTAATTG AGTGCGGCTG GAAGGATCAG 
CTAAAGGCAC ACTGTAAAGA GGTAATTAAA GAAAAAGGAC TAGAACACGT TACTGTTGAT 
GACTTGGTGG CTGAAATCAC TCCAAAAGGC AGAGCCCTGG TACCTGACAG TGTAAAGAAG 
GAGCTCCTAC AGAGAATAAG AACATTCCTT GCTCAGCATG CCAGCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520821.1
CDS	100..390
Translation	MNKDAQMRAAINQKLIETGERERLKELLRAKLIECGWKDQLKAHCKEVIKEKGLEHVTVDDLVAEITPKGRALVPDSVKKELLQRIRTFLAQHASL

Target ORF information:

RefSeq Version	XM_006520758.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus enhancer of yellow 2 homolog (Drosophila) (Eny2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520758.2

ORF Insert Sequence:

ATGAACAAAG ATGCGCAGAT GAGAGCAGCG ATTAACCAAA AGTTAATAGA AACTGGAGAA 
AGAGAACGCC TCAAAGAGTT GCTGAGAGCT AAATTAATTG AGTGCGGCTG GAAGGATCAG 
CTAAAGGCAC ACTGTAAAGA GGTAATTAAA GAAAAAGGAC TAGAACACGT TACTGTTGAT 
GACTTGGTGG CTGAAATCAC TCCAAAAGGC AGAGCCCTGG TACCTGACAG TGTAAAGAAG 
GAGCTCCTAC AGAGAATAAG AACATTCCTT GCTCAGCATG CCAGCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eny2 ( XM_006520758.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520821.1 Mus musculus ENY2 transcription and export complex 2 subunit (Eny2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu51163
Clone ID Related Accession (Same CDS sequence)	XM_006520758.2 , XM_006520758.3
Accession Version	XM_006520758.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transcription and mRNA export factor ENY2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520758.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG ATGCGCAGAT GAGAGCAGCG ATTAACCAAA AGTTAATAGA AACTGGAGAA 
AGAGAACGCC TCAAAGAGTT GCTGAGAGCT AAATTAATTG AGTGCGGCTG GAAGGATCAG 
CTAAAGGCAC ACTGTAAAGA GGTAATTAAA GAAAAAGGAC TAGAACACGT TACTGTTGAT 
GACTTGGTGG CTGAAATCAC TCCAAAAGGC AGAGCCCTGG TACCTGACAG TGTAAAGAAG 
GAGCTCCTAC AGAGAATAAG AACATTCCTT GCTCAGCATG CCAGCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520821.1
CDS	99..389
Translation	MNKDAQMRAAINQKLIETGERERLKELLRAKLIECGWKDQLKAHCKEVIKEKGLEHVTVDDLVAEITPKGRALVPDSVKKELLQRIRTFLAQHASL

Target ORF information:

RefSeq Version	XM_006520758.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ENY2 transcription and export complex 2 subunit (Eny2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520758.3

ORF Insert Sequence:

ATGAACAAAG ATGCGCAGAT GAGAGCAGCG ATTAACCAAA AGTTAATAGA AACTGGAGAA 
AGAGAACGCC TCAAAGAGTT GCTGAGAGCT AAATTAATTG AGTGCGGCTG GAAGGATCAG 
CTAAAGGCAC ACTGTAAAGA GGTAATTAAA GAAAAAGGAC TAGAACACGT TACTGTTGAT 
GACTTGGTGG CTGAAATCAC TCCAAAAGGC AGAGCCCTGG TACCTGACAG TGTAAAGAAG 
GAGCTCCTAC AGAGAATAAG AACATTCCTT GCTCAGCATG CCAGCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Suppression of the nuclear factor Eny2 increases insulin secretion in poorly functioning INS-1E insulinoma cells.
Experimental diabetes research2012460869(2012)
Dames P,Weise M,Puff R,G?ke B,Parhofer KG,Seissler J,Lechner A

Evolutionarily conserved E(y)2/Sus1 protein is essential for the barrier activity of Su(Hw)-dependent insulators in Drosophila.
Molecular cell27(2)332-8(2007 Jul)
Kurshakova M,Maksimenko O,Golovnin A,Pulina M,Georgieva S,Georgiev P,Krasnov A

Analysis of gene expression in the developing mouse retina.
Proceedings of the National Academy of Sciences of the United States of America100(9)5491-6(2003 Apr)
D?az E,Yang YH,Ferreira T,Loh KC,Okazaki Y,Hayashizaki Y,Tessier-Lavigne M,Speed TP,Ngai J

The novel transcription factor e(y)2 interacts with TAF(II)40 and potentiates transcription activation on chromatin templates.
Molecular and cellular biology21(15)5223-31(2001 Aug)
Georgieva S,Nabirochkina E,Dilworth FJ,Eickhoff H,Becker P,Tora L,Georgiev P,Soldatov A

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Eny2 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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