Med16 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Med16
Entrez Gene ID	216154
Full Name	mediator complex subunit 16
Synonyms	95kDa,A630083L04,DRIP92,Thrap5,Trap95
General protein information	Preferred Names mediator complex subunit 16 Names mediator of RNA polymerase II transcription subunit 16 thyroid hormone receptor associated protein 5 thyroid hormone receptor-associated protein complex 95 kDa component thyroid hormone receptor-associated protein, 95-kD subunit vitamin D3 receptor-interacting protein complex 92 kDa component
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_198107.2	NP_932775.2	mediator of RNA polymerase II transcription subunit 16 isoform 1
NM_001163276.1	NP_001156748.1	mediator of RNA polymerase II transcription subunit 16 isoform 2
XM_006513488.3	XP_006513551.1	mediator of RNA polymerase II transcription subunit 16 isoform X2
NM_001359629.1	NP_001346558.1	mediator of RNA polymerase II transcription subunit 16 isoform 3
XM_006513487.4	XP_006513550.1	mediator of RNA polymerase II transcription subunit 16 isoform X1
XM_006513487.3	XP_006513550.1	mediator of RNA polymerase II transcription subunit 16 isoform X2
XM_011243402.2	XP_011241704.1	mediator of RNA polymerase II transcription subunit 16 isoform X1
XM_006513488.4	XP_006513551.1	mediator of RNA polymerase II transcription subunit 16 isoform X1

Pathways from BioSystems

Homologies

	MED16	XP_001117192.2
Canis lupus familiaris (dog)	MED16	XP_005633219.1
Mus musculus (house mouse)	Med16	NP_932775.2
Xenopus tropicalis (tropical clawed frog)	med16	NP_001072641.1
Homo sapiens (human)	MED16	NP_005472.2
Pan troglodytes (chimpanzee)	MED16	XP_512222.3
Bos taurus (cattle)	MED16	XP_002689212.2
Rattus norvegicus (Norway rat)	Med16	NP_001100238.1
Gallus gallus (chicken)	MED16	XP_418220.2
Drosophila melanogaster (fruit fly)	MED16	NP_611650.2
Danio rerio (zebrafish)	med16	NP_001186801.1

Gene Ontology
Bibliography

Related articles in PubMed

The Mediator Subunit MED16 Transduces NRF2-Activating Signals into Antioxidant Gene Expression.
Sekine H, Okazaki K, Ota N, Shima H, Katoh Y, Suzuki N, Igarashi K, Ito M, Motohashi H, Yamamoto M
Molecular and cellular biology36(3)407-20(2015 Nov)

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
Krebs AR, Demmers J, Karmodiya K, Chang NC, Chang AC, Tora L
EMBO reports11(7)541-7(2010 Jul)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Diversification of stem cell molecular repertoire by alternative splicing.
Pritsker M, Doniger TT, Kramer LC, Westcot SE, Lemischka IR
Proceedings of the National Academy of Sciences of the United States of America102(40)14290-5(2005 Oct)

Structural and functional organization of TRAP220, the TRAP/mediator subunit that is targeted by nuclear receptors.
Malik S, Guermah M, Yuan CX, Wu W, Yamamura S, Roeder RG
Molecular and cellular biology24(18)8244-54(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NRF2-dependent phosphorylation of the RNA polymerase II C-terminal domain was absent in Med16-deficient cells, suggesting that MED16 serves as a conduit to transmit NRF2-activating signals to RNA polymerase II.
Title: The Mediator Subunit MED16 Transduces NRF2-Activating Signals into Antioxidant Gene Expression.

The following Med16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu44386	XM_006513487.4 Latest version!	Mus musculus mediator complex subunit 16 (Med16), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu44386	XM_006513488.4 Latest version!	Mus musculus mediator complex subunit 16 (Med16), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu08693	NM_198107.2 Latest version!	Mus musculus mediator complex subunit 16 (Med16), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OMu08852	NM_001163276.1 Latest version!	Mus musculus mediator complex subunit 16 (Med16), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu72948	NM_001359629.1 Latest version!	Mus musculus mediator complex subunit 16 (Med16), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu72948	XM_011243402.2 Latest version!	Mus musculus mediator complex subunit 16 (Med16), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OMu44386	XM_006513487.3	Mus musculus mediator complex subunit 16 (Med16), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu44386	XM_006513488.3	Mus musculus mediator complex subunit 16 (Med16), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med16 ( XM_006513488.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513551.1 Mus musculus mediator complex subunit 16 (Med16), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu44386
Clone ID Related Accession (Same CDS sequence)	XM_006513488.3 , XM_006513487.4 , XM_006513487.3 , XM_006513488.4
Accession Version	XM_006513488.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 16 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513488.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACC TGGCCTATGT GTGCGAATGG GAGAAGTGGG CCAAGAGCAC GTACTGCCCG 
TCGCTGCCCC TGGCTTGTGC CTGGTCCTGC AGGAACCTCA TTGCCTTCAC CACAGACCTA 
CGCAATGATG ACCAGGATCT GACACACATG ATCCATATCC TAGACACGGA ACACCCCTGG 
GAAGTGCACT CTGTCAGCTC TGGCCACAGC GAGGCCATCA CCTGCCTGGA GTGGGACCAG 
TCAGGGTCCC GGCTACTGTC TGCTGATGCT GATGGACAGA TCAAATGCTG GAGCATGGCT 
GACCACCTGG CCAACAGCTG GGAGAGCTCG GTGGGCAGCC AGGTGGAGGG GGACCCCATC 
GTGGCCCTGT CCTGGCTGCA CAACGGTGTA AAGCTGGCCC TGCATGTGGA GAAGTCTGGT 
GCCTCTAGCT TCGGAGAGAA GTTCTCACGT GTGAAGTTCT CCCCGTCCCT CACGCTGTTC 
GGTGGCAAGC CAATGGAGGG TTGGATTGCA GTGACAGTCA GTGGCCTGGT CACTGTGTCC 
CTTCTGAAGC CCAGCGGGCA GGTGCTGACG TCCACGGAGA GCCTGTGCCG GCTGCGTGGT 
AGAGTGGCGC TGGCTGACAT CGCCTTCACG GGCGGTGGGA ACATTGTGGT GGCTGCTGCA 
GATGGCAGCA GCGCGTCACC CGTGAAGTTC TATAAGGTGT GCGTCAGCGT GGTCAGCGAG 
AAGTGCCGCA TCGACACGGA GATCCTGCCC TCACTGTTTA TGCGCTGCAC CACCGACCCC 
AACCGCAAAG ACAGGTTCCC CGCCATCACA CACCTCAAGT TCCTGGCGCG AGACATGTCT 
GAGCAGGTGC TCCTGTGTGC GTCCAGCCAG ACCAGCAGCC TGGTGGAATG CTGGTCCCTT 
CGGAAAGAGG GCCTTCCTGT GAACAATATC TTCCAGCAGA TCTCGCCTGT GGTTGGTGAC 
AAACAGCCCA TGATCCTCAA GTGGCGAATC CTGTCGGCCA CCAATGACCT GGACCGTGTA 
TCCGCGGTGG CACTGCCGAA ACTGCCCATC TCACTCACCA ACACTGACCT CAAGGTGGCC 
AGTGACACCC AGTTCTATCC AGGCCTTGGC TTAGCGCTGG CCTTCCAGGA TGGCAGCGTG 
CACATGGTGC ACCGACTGTC CCTGCAGACC ATGGCGGTGT TCTACAGCTC AGCCCCGCGC 
TCGCTGGATG AGCCGGCCCT AAAGCGCCCA CGCACCACAT GCCCTGCCGT ACACTTTAAA 
GCCATGCAGC TGTCCTGGAC TTCGCTGGCC CTCGTGGGCA TCGATAACCA TGGGAAGCTC 
AGCATGCTGC GAATCTCTCC ATCCCTGGGC CACCCACTGG AGCCCAAGCT GGCCCTGCAG 
CACCTGCTTT TCCTGCTGGA ATACTGCATG GTGACCGGCT ATGACTGGTG GGACATCCTG 
CTACACGTGC AGCCCGGCAT GGTGCAGAGC CTGGTGGAGC GGCTGCATGA GGAGTACACT 
CGCCAGAAGC CCGCCCTGCA ACAGGTCCTC TCCACTCGGA TCCTGGCCAT GAAGGCTTCA 
CTGTGCAAGC TGTCACCCTG CACAGTGGCT CGTGTGTGTG ACTACCACAC CAAGCTGTTC 
CTCATGGCCA TCACGTCCAC CCTAAAGTCG CTGCTGCGCC CACACTTCCT CAACACCCCT 
GACAAGAGCC CTGGGGACCG CCTGGCCGAG ATCTGCGCCA AGATCACCGA TGTTGACATC 
GACAAAGTCA TGATCAACCT GAAGACTGAA GAGTTCGTCC TTGACATGAA CACTTTGCAG 
GCGCTGCAGC AGTTGCTACA GTGGGTGGGA GACTTCGTGC TCTACCTCCT GGTCAGCCTG 
CCCAACCAGG GCTCCCCGCT AAGGCCGGGC CACAGCTTCC TCCGAGATGG TACCTCCCTG 
GGCATGCTGC GAGAGTTGAT GGTTGTCATC CGAATCTGGG GCCTGCTGAA GCCCAGCTGT 
CTGCCCGTCT ACACAGCCAC CTCGGACACC CAGGACAGCA TGTCCCTGCT CTTCCGACTG 
CTCACCAAAC TGTGGATCTG CTGCCGTGAT GAGGGCGCAG CCAGTGAACC AGATGAGGGT 
TTGGTGGATG AATGCTGCCT GCTGCCTAGC CAGCTGCTGG TCCCCAACTT GGACTGGCTT 
CCCGCCAGTG ATGGCCTGGT CAGTCGCCTG CAGCCCAAAC AGCCCCTGCG CCTGCGCTTC 
GGCAGAGCGC CCACTCTGCC CAGCAGCACC TCCACCCTGC AGCTGGACGG CCTCACCAGG 
GCCCCTGGCC AGCCCAAGAT CGACCACCTC CGGAGGCTAC ACCTGGGCGC CTACCCCACC 
GAGGAGTGCA AAGCCTGCAC CAGGTGTGGC TGTGTCACCA TGCTGAAGTC TCCCAACAAG 
ACAACCGCTG TGACACAGTG GGAACAGCGG TGGATCAAGA ATTGCCTGTG TGGTGGGCTG 
TGGCGGCGAG TGCCTCTCAG CTGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513551.1
CDS	130..2619
Translation	MMDLAYVCEWEKWAKSTYCPSLPLACAWSCRNLIAFTTDLRNDDQDLTHMIHILDTEHPWEVHSVSSGHSEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWESSVGSQVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVAAADGSSASPVKFYKVCVSVVSEKCRIDTEILPSLFMRCTTDPNRKDRFPAITHLKFLARDMSEQVLLCASSQTSSLVECWSLRKEGLPVNNIFQQISPVVGDKQPMILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFQDGSVHMVHRLSLQTMAVFYSSAPRSLDEPALKRPRTTCPAVHFKAMQLSWTSLALVGIDNHGKLSMLRISPSLGHPLEPKLALQHLLFLLEYCMVTGYDWWDILLHVQPGMVQSLVERLHEEYTRQKPALQQVLSTRILAMKASLCKLSPCTVARVCDYHTKLFLMAITSTLKSLLRPHFLNTPDKSPGDRLAEICAKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLVSLPNQGSPLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICCRDEGAASEPDEGLVDECCLLPSQLLVPNLDWLPASDGLVSRLQPKQPLRLRFGRAPTLPSSTSTLQLDGLTRAPGQPKIDHLRRLHLGAYPTEECKACTRCGCVTMLKSPNKTTAVTQWEQRWIKNCLCGGLWRRVPLSCS

Target ORF information:

RefSeq Version	XM_006513488.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 16 (Med16), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513488.3

ORF Insert Sequence:

ATGATGGACC TGGCCTATGT GTGCGAATGG GAGAAGTGGG CCAAGAGCAC GTACTGCCCG 
TCGCTGCCCC TGGCTTGTGC CTGGTCCTGC AGGAACCTCA TTGCCTTCAC CACAGACCTA 
CGCAATGATG ACCAGGATCT GACACACATG ATCCATATCC TAGACACGGA ACACCCCTGG 
GAAGTGCACT CTGTCAGCTC TGGCCACAGC GAGGCCATCA CCTGCCTGGA GTGGGACCAG 
TCAGGGTCCC GGCTACTGTC TGCTGATGCT GATGGACAGA TCAAATGCTG GAGCATGGCT 
GACCACCTGG CCAACAGCTG GGAGAGCTCG GTGGGCAGCC AGGTGGAGGG GGACCCCATC 
GTGGCCCTGT CCTGGCTGCA CAACGGTGTA AAGCTGGCCC TGCATGTGGA GAAGTCTGGT 
GCCTCTAGCT TCGGAGAGAA GTTCTCACGT GTGAAGTTCT CCCCGTCCCT CACGCTGTTC 
GGTGGCAAGC CAATGGAGGG TTGGATTGCA GTGACAGTCA GTGGCCTGGT CACTGTGTCC 
CTTCTGAAGC CCAGCGGGCA GGTGCTGACG TCCACGGAGA GCCTGTGCCG GCTGCGTGGT 
AGAGTGGCGC TGGCTGACAT CGCCTTCACG GGCGGTGGGA ACATTGTGGT GGCTGCTGCA 
GATGGCAGCA GCGCGTCACC CGTGAAGTTC TATAAGGTGT GCGTCAGCGT GGTCAGCGAG 
AAGTGCCGCA TCGACACGGA GATCCTGCCC TCACTGTTTA TGCGCTGCAC CACCGACCCC 
AACCGCAAAG ACAGGTTCCC CGCCATCACA CACCTCAAGT TCCTGGCGCG AGACATGTCT 
GAGCAGGTGC TCCTGTGTGC GTCCAGCCAG ACCAGCAGCC TGGTGGAATG CTGGTCCCTT 
CGGAAAGAGG GCCTTCCTGT GAACAATATC TTCCAGCAGA TCTCGCCTGT GGTTGGTGAC 
AAACAGCCCA TGATCCTCAA GTGGCGAATC CTGTCGGCCA CCAATGACCT GGACCGTGTA 
TCCGCGGTGG CACTGCCGAA ACTGCCCATC TCACTCACCA ACACTGACCT CAAGGTGGCC 
AGTGACACCC AGTTCTATCC AGGCCTTGGC TTAGCGCTGG CCTTCCAGGA TGGCAGCGTG 
CACATGGTGC ACCGACTGTC CCTGCAGACC ATGGCGGTGT TCTACAGCTC AGCCCCGCGC 
TCGCTGGATG AGCCGGCCCT AAAGCGCCCA CGCACCACAT GCCCTGCCGT ACACTTTAAA 
GCCATGCAGC TGTCCTGGAC TTCGCTGGCC CTCGTGGGCA TCGATAACCA TGGGAAGCTC 
AGCATGCTGC GAATCTCTCC ATCCCTGGGC CACCCACTGG AGCCCAAGCT GGCCCTGCAG 
CACCTGCTTT TCCTGCTGGA ATACTGCATG GTGACCGGCT ATGACTGGTG GGACATCCTG 
CTACACGTGC AGCCCGGCAT GGTGCAGAGC CTGGTGGAGC GGCTGCATGA GGAGTACACT 
CGCCAGAAGC CCGCCCTGCA ACAGGTCCTC TCCACTCGGA TCCTGGCCAT GAAGGCTTCA 
CTGTGCAAGC TGTCACCCTG CACAGTGGCT CGTGTGTGTG ACTACCACAC CAAGCTGTTC 
CTCATGGCCA TCACGTCCAC CCTAAAGTCG CTGCTGCGCC CACACTTCCT CAACACCCCT 
GACAAGAGCC CTGGGGACCG CCTGGCCGAG ATCTGCGCCA AGATCACCGA TGTTGACATC 
GACAAAGTCA TGATCAACCT GAAGACTGAA GAGTTCGTCC TTGACATGAA CACTTTGCAG 
GCGCTGCAGC AGTTGCTACA GTGGGTGGGA GACTTCGTGC TCTACCTCCT GGTCAGCCTG 
CCCAACCAGG GCTCCCCGCT AAGGCCGGGC CACAGCTTCC TCCGAGATGG TACCTCCCTG 
GGCATGCTGC GAGAGTTGAT GGTTGTCATC CGAATCTGGG GCCTGCTGAA GCCCAGCTGT 
CTGCCCGTCT ACACAGCCAC CTCGGACACC CAGGACAGCA TGTCCCTGCT CTTCCGACTG 
CTCACCAAAC TGTGGATCTG CTGCCGTGAT GAGGGCGCAG CCAGTGAACC AGATGAGGGT 
TTGGTGGATG AATGCTGCCT GCTGCCTAGC CAGCTGCTGG TCCCCAACTT GGACTGGCTT 
CCCGCCAGTG ATGGCCTGGT CAGTCGCCTG CAGCCCAAAC AGCCCCTGCG CCTGCGCTTC 
GGCAGAGCGC CCACTCTGCC CAGCAGCACC TCCACCCTGC AGCTGGACGG CCTCACCAGG 
GCCCCTGGCC AGCCCAAGAT CGACCACCTC CGGAGGCTAC ACCTGGGCGC CTACCCCACC 
GAGGAGTGCA AAGCCTGCAC CAGGTGTGGC TGTGTCACCA TGCTGAAGTC TCCCAACAAG 
ACAACCGCTG TGACACAGTG GGAACAGCGG TGGATCAAGA ATTGCCTGTG TGGTGGGCTG 
TGGCGGCGAG TGCCTCTCAG CTGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med16 ( XM_006513487.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513550.1 Mus musculus mediator complex subunit 16 (Med16), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu44386
Clone ID Related Accession (Same CDS sequence)	XM_006513488.3 , XM_006513487.4 , XM_006513487.3 , XM_006513488.4
Accession Version	XM_006513487.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 16 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACC TGGCCTATGT GTGCGAATGG GAGAAGTGGG CCAAGAGCAC GTACTGCCCG 
TCGCTGCCCC TGGCTTGTGC CTGGTCCTGC AGGAACCTCA TTGCCTTCAC CACAGACCTA 
CGCAATGATG ACCAGGATCT GACACACATG ATCCATATCC TAGACACGGA ACACCCCTGG 
GAAGTGCACT CTGTCAGCTC TGGCCACAGC GAGGCCATCA CCTGCCTGGA GTGGGACCAG 
TCAGGGTCCC GGCTACTGTC TGCTGATGCT GATGGACAGA TCAAATGCTG GAGCATGGCT 
GACCACCTGG CCAACAGCTG GGAGAGCTCG GTGGGCAGCC AGGTGGAGGG GGACCCCATC 
GTGGCCCTGT CCTGGCTGCA CAACGGTGTA AAGCTGGCCC TGCATGTGGA GAAGTCTGGT 
GCCTCTAGCT TCGGAGAGAA GTTCTCACGT GTGAAGTTCT CCCCGTCCCT CACGCTGTTC 
GGTGGCAAGC CAATGGAGGG TTGGATTGCA GTGACAGTCA GTGGCCTGGT CACTGTGTCC 
CTTCTGAAGC CCAGCGGGCA GGTGCTGACG TCCACGGAGA GCCTGTGCCG GCTGCGTGGT 
AGAGTGGCGC TGGCTGACAT CGCCTTCACG GGCGGTGGGA ACATTGTGGT GGCTGCTGCA 
GATGGCAGCA GCGCGTCACC CGTGAAGTTC TATAAGGTGT GCGTCAGCGT GGTCAGCGAG 
AAGTGCCGCA TCGACACGGA GATCCTGCCC TCACTGTTTA TGCGCTGCAC CACCGACCCC 
AACCGCAAAG ACAGGTTCCC CGCCATCACA CACCTCAAGT TCCTGGCGCG AGACATGTCT 
GAGCAGGTGC TCCTGTGTGC GTCCAGCCAG ACCAGCAGCC TGGTGGAATG CTGGTCCCTT 
CGGAAAGAGG GCCTTCCTGT GAACAATATC TTCCAGCAGA TCTCGCCTGT GGTTGGTGAC 
AAACAGCCCA TGATCCTCAA GTGGCGAATC CTGTCGGCCA CCAATGACCT GGACCGTGTA 
TCCGCGGTGG CACTGCCGAA ACTGCCCATC TCACTCACCA ACACTGACCT CAAGGTGGCC 
AGTGACACCC AGTTCTATCC AGGCCTTGGC TTAGCGCTGG CCTTCCAGGA TGGCAGCGTG 
CACATGGTGC ACCGACTGTC CCTGCAGACC ATGGCGGTGT TCTACAGCTC AGCCCCGCGC 
TCGCTGGATG AGCCGGCCCT AAAGCGCCCA CGCACCACAT GCCCTGCCGT ACACTTTAAA 
GCCATGCAGC TGTCCTGGAC TTCGCTGGCC CTCGTGGGCA TCGATAACCA TGGGAAGCTC 
AGCATGCTGC GAATCTCTCC ATCCCTGGGC CACCCACTGG AGCCCAAGCT GGCCCTGCAG 
CACCTGCTTT TCCTGCTGGA ATACTGCATG GTGACCGGCT ATGACTGGTG GGACATCCTG 
CTACACGTGC AGCCCGGCAT GGTGCAGAGC CTGGTGGAGC GGCTGCATGA GGAGTACACT 
CGCCAGAAGC CCGCCCTGCA ACAGGTCCTC TCCACTCGGA TCCTGGCCAT GAAGGCTTCA 
CTGTGCAAGC TGTCACCCTG CACAGTGGCT CGTGTGTGTG ACTACCACAC CAAGCTGTTC 
CTCATGGCCA TCACGTCCAC CCTAAAGTCG CTGCTGCGCC CACACTTCCT CAACACCCCT 
GACAAGAGCC CTGGGGACCG CCTGGCCGAG ATCTGCGCCA AGATCACCGA TGTTGACATC 
GACAAAGTCA TGATCAACCT GAAGACTGAA GAGTTCGTCC TTGACATGAA CACTTTGCAG 
GCGCTGCAGC AGTTGCTACA GTGGGTGGGA GACTTCGTGC TCTACCTCCT GGTCAGCCTG 
CCCAACCAGG GCTCCCCGCT AAGGCCGGGC CACAGCTTCC TCCGAGATGG TACCTCCCTG 
GGCATGCTGC GAGAGTTGAT GGTTGTCATC CGAATCTGGG GCCTGCTGAA GCCCAGCTGT 
CTGCCCGTCT ACACAGCCAC CTCGGACACC CAGGACAGCA TGTCCCTGCT CTTCCGACTG 
CTCACCAAAC TGTGGATCTG CTGCCGTGAT GAGGGCGCAG CCAGTGAACC AGATGAGGGT 
TTGGTGGATG AATGCTGCCT GCTGCCTAGC CAGCTGCTGG TCCCCAACTT GGACTGGCTT 
CCCGCCAGTG ATGGCCTGGT CAGTCGCCTG CAGCCCAAAC AGCCCCTGCG CCTGCGCTTC 
GGCAGAGCGC CCACTCTGCC CAGCAGCACC TCCACCCTGC AGCTGGACGG CCTCACCAGG 
GCCCCTGGCC AGCCCAAGAT CGACCACCTC CGGAGGCTAC ACCTGGGCGC CTACCCCACC 
GAGGAGTGCA AAGCCTGCAC CAGGTGTGGC TGTGTCACCA TGCTGAAGTC TCCCAACAAG 
ACAACCGCTG TGACACAGTG GGAACAGCGG TGGATCAAGA ATTGCCTGTG TGGTGGGCTG 
TGGCGGCGAG TGCCTCTCAG CTGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513550.1
CDS	116..2605
Translation	MMDLAYVCEWEKWAKSTYCPSLPLACAWSCRNLIAFTTDLRNDDQDLTHMIHILDTEHPWEVHSVSSGHSEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWESSVGSQVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVAAADGSSASPVKFYKVCVSVVSEKCRIDTEILPSLFMRCTTDPNRKDRFPAITHLKFLARDMSEQVLLCASSQTSSLVECWSLRKEGLPVNNIFQQISPVVGDKQPMILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFQDGSVHMVHRLSLQTMAVFYSSAPRSLDEPALKRPRTTCPAVHFKAMQLSWTSLALVGIDNHGKLSMLRISPSLGHPLEPKLALQHLLFLLEYCMVTGYDWWDILLHVQPGMVQSLVERLHEEYTRQKPALQQVLSTRILAMKASLCKLSPCTVARVCDYHTKLFLMAITSTLKSLLRPHFLNTPDKSPGDRLAEICAKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLVSLPNQGSPLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICCRDEGAASEPDEGLVDECCLLPSQLLVPNLDWLPASDGLVSRLQPKQPLRLRFGRAPTLPSSTSTLQLDGLTRAPGQPKIDHLRRLHLGAYPTEECKACTRCGCVTMLKSPNKTTAVTQWEQRWIKNCLCGGLWRRVPLSCS

Target ORF information:

RefSeq Version	XM_006513487.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 16 (Med16), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513487.3

ORF Insert Sequence:

ATGATGGACC TGGCCTATGT GTGCGAATGG GAGAAGTGGG CCAAGAGCAC GTACTGCCCG 
TCGCTGCCCC TGGCTTGTGC CTGGTCCTGC AGGAACCTCA TTGCCTTCAC CACAGACCTA 
CGCAATGATG ACCAGGATCT GACACACATG ATCCATATCC TAGACACGGA ACACCCCTGG 
GAAGTGCACT CTGTCAGCTC TGGCCACAGC GAGGCCATCA CCTGCCTGGA GTGGGACCAG 
TCAGGGTCCC GGCTACTGTC TGCTGATGCT GATGGACAGA TCAAATGCTG GAGCATGGCT 
GACCACCTGG CCAACAGCTG GGAGAGCTCG GTGGGCAGCC AGGTGGAGGG GGACCCCATC 
GTGGCCCTGT CCTGGCTGCA CAACGGTGTA AAGCTGGCCC TGCATGTGGA GAAGTCTGGT 
GCCTCTAGCT TCGGAGAGAA GTTCTCACGT GTGAAGTTCT CCCCGTCCCT CACGCTGTTC 
GGTGGCAAGC CAATGGAGGG TTGGATTGCA GTGACAGTCA GTGGCCTGGT CACTGTGTCC 
CTTCTGAAGC CCAGCGGGCA GGTGCTGACG TCCACGGAGA GCCTGTGCCG GCTGCGTGGT 
AGAGTGGCGC TGGCTGACAT CGCCTTCACG GGCGGTGGGA ACATTGTGGT GGCTGCTGCA 
GATGGCAGCA GCGCGTCACC CGTGAAGTTC TATAAGGTGT GCGTCAGCGT GGTCAGCGAG 
AAGTGCCGCA TCGACACGGA GATCCTGCCC TCACTGTTTA TGCGCTGCAC CACCGACCCC 
AACCGCAAAG ACAGGTTCCC CGCCATCACA CACCTCAAGT TCCTGGCGCG AGACATGTCT 
GAGCAGGTGC TCCTGTGTGC GTCCAGCCAG ACCAGCAGCC TGGTGGAATG CTGGTCCCTT 
CGGAAAGAGG GCCTTCCTGT GAACAATATC TTCCAGCAGA TCTCGCCTGT GGTTGGTGAC 
AAACAGCCCA TGATCCTCAA GTGGCGAATC CTGTCGGCCA CCAATGACCT GGACCGTGTA 
TCCGCGGTGG CACTGCCGAA ACTGCCCATC TCACTCACCA ACACTGACCT CAAGGTGGCC 
AGTGACACCC AGTTCTATCC AGGCCTTGGC TTAGCGCTGG CCTTCCAGGA TGGCAGCGTG 
CACATGGTGC ACCGACTGTC CCTGCAGACC ATGGCGGTGT TCTACAGCTC AGCCCCGCGC 
TCGCTGGATG AGCCGGCCCT AAAGCGCCCA CGCACCACAT GCCCTGCCGT ACACTTTAAA 
GCCATGCAGC TGTCCTGGAC TTCGCTGGCC CTCGTGGGCA TCGATAACCA TGGGAAGCTC 
AGCATGCTGC GAATCTCTCC ATCCCTGGGC CACCCACTGG AGCCCAAGCT GGCCCTGCAG 
CACCTGCTTT TCCTGCTGGA ATACTGCATG GTGACCGGCT ATGACTGGTG GGACATCCTG 
CTACACGTGC AGCCCGGCAT GGTGCAGAGC CTGGTGGAGC GGCTGCATGA GGAGTACACT 
CGCCAGAAGC CCGCCCTGCA ACAGGTCCTC TCCACTCGGA TCCTGGCCAT GAAGGCTTCA 
CTGTGCAAGC TGTCACCCTG CACAGTGGCT CGTGTGTGTG ACTACCACAC CAAGCTGTTC 
CTCATGGCCA TCACGTCCAC CCTAAAGTCG CTGCTGCGCC CACACTTCCT CAACACCCCT 
GACAAGAGCC CTGGGGACCG CCTGGCCGAG ATCTGCGCCA AGATCACCGA TGTTGACATC 
GACAAAGTCA TGATCAACCT GAAGACTGAA GAGTTCGTCC TTGACATGAA CACTTTGCAG 
GCGCTGCAGC AGTTGCTACA GTGGGTGGGA GACTTCGTGC TCTACCTCCT GGTCAGCCTG 
CCCAACCAGG GCTCCCCGCT AAGGCCGGGC CACAGCTTCC TCCGAGATGG TACCTCCCTG 
GGCATGCTGC GAGAGTTGAT GGTTGTCATC CGAATCTGGG GCCTGCTGAA GCCCAGCTGT 
CTGCCCGTCT ACACAGCCAC CTCGGACACC CAGGACAGCA TGTCCCTGCT CTTCCGACTG 
CTCACCAAAC TGTGGATCTG CTGCCGTGAT GAGGGCGCAG CCAGTGAACC AGATGAGGGT 
TTGGTGGATG AATGCTGCCT GCTGCCTAGC CAGCTGCTGG TCCCCAACTT GGACTGGCTT 
CCCGCCAGTG ATGGCCTGGT CAGTCGCCTG CAGCCCAAAC AGCCCCTGCG CCTGCGCTTC 
GGCAGAGCGC CCACTCTGCC CAGCAGCACC TCCACCCTGC AGCTGGACGG CCTCACCAGG 
GCCCCTGGCC AGCCCAAGAT CGACCACCTC CGGAGGCTAC ACCTGGGCGC CTACCCCACC 
GAGGAGTGCA AAGCCTGCAC CAGGTGTGGC TGTGTCACCA TGCTGAAGTC TCCCAACAAG 
ACAACCGCTG TGACACAGTG GGAACAGCGG TGGATCAAGA ATTGCCTGTG TGGTGGGCTG 
TGGCGGCGAG TGCCTCTCAG CTGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med16 ( XM_006513487.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513550.1 Mus musculus mediator complex subunit 16 (Med16), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu44386
Clone ID Related Accession (Same CDS sequence)	XM_006513488.3 , XM_006513487.4 , XM_006513487.3 , XM_006513488.4
Accession Version	XM_006513487.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513487.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACC TGGCCTATGT GTGCGAATGG GAGAAGTGGG CCAAGAGCAC GTACTGCCCG 
TCGCTGCCCC TGGCTTGTGC CTGGTCCTGC AGGAACCTCA TTGCCTTCAC CACAGACCTA 
CGCAATGATG ACCAGGATCT GACACACATG ATCCATATCC TAGACACGGA ACACCCCTGG 
GAAGTGCACT CTGTCAGCTC TGGCCACAGC GAGGCCATCA CCTGCCTGGA GTGGGACCAG 
TCAGGGTCCC GGCTACTGTC TGCTGATGCT GATGGACAGA TCAAATGCTG GAGCATGGCT 
GACCACCTGG CCAACAGCTG GGAGAGCTCG GTGGGCAGCC AGGTGGAGGG GGACCCCATC 
GTGGCCCTGT CCTGGCTGCA CAACGGTGTA AAGCTGGCCC TGCATGTGGA GAAGTCTGGT 
GCCTCTAGCT TCGGAGAGAA GTTCTCACGT GTGAAGTTCT CCCCGTCCCT CACGCTGTTC 
GGTGGCAAGC CAATGGAGGG TTGGATTGCA GTGACAGTCA GTGGCCTGGT CACTGTGTCC 
CTTCTGAAGC CCAGCGGGCA GGTGCTGACG TCCACGGAGA GCCTGTGCCG GCTGCGTGGT 
AGAGTGGCGC TGGCTGACAT CGCCTTCACG GGCGGTGGGA ACATTGTGGT GGCTGCTGCA 
GATGGCAGCA GCGCGTCACC CGTGAAGTTC TATAAGGTGT GCGTCAGCGT GGTCAGCGAG 
AAGTGCCGCA TCGACACGGA GATCCTGCCC TCACTGTTTA TGCGCTGCAC CACCGACCCC 
AACCGCAAAG ACAGGTTCCC CGCCATCACA CACCTCAAGT TCCTGGCGCG AGACATGTCT 
GAGCAGGTGC TCCTGTGTGC GTCCAGCCAG ACCAGCAGCC TGGTGGAATG CTGGTCCCTT 
CGGAAAGAGG GCCTTCCTGT GAACAATATC TTCCAGCAGA TCTCGCCTGT GGTTGGTGAC 
AAACAGCCCA TGATCCTCAA GTGGCGAATC CTGTCGGCCA CCAATGACCT GGACCGTGTA 
TCCGCGGTGG CACTGCCGAA ACTGCCCATC TCACTCACCA ACACTGACCT CAAGGTGGCC 
AGTGACACCC AGTTCTATCC AGGCCTTGGC TTAGCGCTGG CCTTCCAGGA TGGCAGCGTG 
CACATGGTGC ACCGACTGTC CCTGCAGACC ATGGCGGTGT TCTACAGCTC AGCCCCGCGC 
TCGCTGGATG AGCCGGCCCT AAAGCGCCCA CGCACCACAT GCCCTGCCGT ACACTTTAAA 
GCCATGCAGC TGTCCTGGAC TTCGCTGGCC CTCGTGGGCA TCGATAACCA TGGGAAGCTC 
AGCATGCTGC GAATCTCTCC ATCCCTGGGC CACCCACTGG AGCCCAAGCT GGCCCTGCAG 
CACCTGCTTT TCCTGCTGGA ATACTGCATG GTGACCGGCT ATGACTGGTG GGACATCCTG 
CTACACGTGC AGCCCGGCAT GGTGCAGAGC CTGGTGGAGC GGCTGCATGA GGAGTACACT 
CGCCAGAAGC CCGCCCTGCA ACAGGTCCTC TCCACTCGGA TCCTGGCCAT GAAGGCTTCA 
CTGTGCAAGC TGTCACCCTG CACAGTGGCT CGTGTGTGTG ACTACCACAC CAAGCTGTTC 
CTCATGGCCA TCACGTCCAC CCTAAAGTCG CTGCTGCGCC CACACTTCCT CAACACCCCT 
GACAAGAGCC CTGGGGACCG CCTGGCCGAG ATCTGCGCCA AGATCACCGA TGTTGACATC 
GACAAAGTCA TGATCAACCT GAAGACTGAA GAGTTCGTCC TTGACATGAA CACTTTGCAG 
GCGCTGCAGC AGTTGCTACA GTGGGTGGGA GACTTCGTGC TCTACCTCCT GGTCAGCCTG 
CCCAACCAGG GCTCCCCGCT AAGGCCGGGC CACAGCTTCC TCCGAGATGG TACCTCCCTG 
GGCATGCTGC GAGAGTTGAT GGTTGTCATC CGAATCTGGG GCCTGCTGAA GCCCAGCTGT 
CTGCCCGTCT ACACAGCCAC CTCGGACACC CAGGACAGCA TGTCCCTGCT CTTCCGACTG 
CTCACCAAAC TGTGGATCTG CTGCCGTGAT GAGGGCGCAG CCAGTGAACC AGATGAGGGT 
TTGGTGGATG AATGCTGCCT GCTGCCTAGC CAGCTGCTGG TCCCCAACTT GGACTGGCTT 
CCCGCCAGTG ATGGCCTGGT CAGTCGCCTG CAGCCCAAAC AGCCCCTGCG CCTGCGCTTC 
GGCAGAGCGC CCACTCTGCC CAGCAGCACC TCCACCCTGC AGCTGGACGG CCTCACCAGG 
GCCCCTGGCC AGCCCAAGAT CGACCACCTC CGGAGGCTAC ACCTGGGCGC CTACCCCACC 
GAGGAGTGCA AAGCCTGCAC CAGGTGTGGC TGTGTCACCA TGCTGAAGTC TCCCAACAAG 
ACAACCGCTG TGACACAGTG GGAACAGCGG TGGATCAAGA ATTGCCTGTG TGGTGGGCTG 
TGGCGGCGAG TGCCTCTCAG CTGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513550.1
CDS	116..2605
Translation	MMDLAYVCEWEKWAKSTYCPSLPLACAWSCRNLIAFTTDLRNDDQDLTHMIHILDTEHPWEVHSVSSGHSEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWESSVGSQVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVAAADGSSASPVKFYKVCVSVVSEKCRIDTEILPSLFMRCTTDPNRKDRFPAITHLKFLARDMSEQVLLCASSQTSSLVECWSLRKEGLPVNNIFQQISPVVGDKQPMILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFQDGSVHMVHRLSLQTMAVFYSSAPRSLDEPALKRPRTTCPAVHFKAMQLSWTSLALVGIDNHGKLSMLRISPSLGHPLEPKLALQHLLFLLEYCMVTGYDWWDILLHVQPGMVQSLVERLHEEYTRQKPALQQVLSTRILAMKASLCKLSPCTVARVCDYHTKLFLMAITSTLKSLLRPHFLNTPDKSPGDRLAEICAKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLVSLPNQGSPLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICCRDEGAASEPDEGLVDECCLLPSQLLVPNLDWLPASDGLVSRLQPKQPLRLRFGRAPTLPSSTSTLQLDGLTRAPGQPKIDHLRRLHLGAYPTEECKACTRCGCVTMLKSPNKTTAVTQWEQRWIKNCLCGGLWRRVPLSCS

Target ORF information:

RefSeq Version	XM_006513487.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 16 (Med16), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513487.4

ORF Insert Sequence:

ATGATGGACC TGGCCTATGT GTGCGAATGG GAGAAGTGGG CCAAGAGCAC GTACTGCCCG 
TCGCTGCCCC TGGCTTGTGC CTGGTCCTGC AGGAACCTCA TTGCCTTCAC CACAGACCTA 
CGCAATGATG ACCAGGATCT GACACACATG ATCCATATCC TAGACACGGA ACACCCCTGG 
GAAGTGCACT CTGTCAGCTC TGGCCACAGC GAGGCCATCA CCTGCCTGGA GTGGGACCAG 
TCAGGGTCCC GGCTACTGTC TGCTGATGCT GATGGACAGA TCAAATGCTG GAGCATGGCT 
GACCACCTGG CCAACAGCTG GGAGAGCTCG GTGGGCAGCC AGGTGGAGGG GGACCCCATC 
GTGGCCCTGT CCTGGCTGCA CAACGGTGTA AAGCTGGCCC TGCATGTGGA GAAGTCTGGT 
GCCTCTAGCT TCGGAGAGAA GTTCTCACGT GTGAAGTTCT CCCCGTCCCT CACGCTGTTC 
GGTGGCAAGC CAATGGAGGG TTGGATTGCA GTGACAGTCA GTGGCCTGGT CACTGTGTCC 
CTTCTGAAGC CCAGCGGGCA GGTGCTGACG TCCACGGAGA GCCTGTGCCG GCTGCGTGGT 
AGAGTGGCGC TGGCTGACAT CGCCTTCACG GGCGGTGGGA ACATTGTGGT GGCTGCTGCA 
GATGGCAGCA GCGCGTCACC CGTGAAGTTC TATAAGGTGT GCGTCAGCGT GGTCAGCGAG 
AAGTGCCGCA TCGACACGGA GATCCTGCCC TCACTGTTTA TGCGCTGCAC CACCGACCCC 
AACCGCAAAG ACAGGTTCCC CGCCATCACA CACCTCAAGT TCCTGGCGCG AGACATGTCT 
GAGCAGGTGC TCCTGTGTGC GTCCAGCCAG ACCAGCAGCC TGGTGGAATG CTGGTCCCTT 
CGGAAAGAGG GCCTTCCTGT GAACAATATC TTCCAGCAGA TCTCGCCTGT GGTTGGTGAC 
AAACAGCCCA TGATCCTCAA GTGGCGAATC CTGTCGGCCA CCAATGACCT GGACCGTGTA 
TCCGCGGTGG CACTGCCGAA ACTGCCCATC TCACTCACCA ACACTGACCT CAAGGTGGCC 
AGTGACACCC AGTTCTATCC AGGCCTTGGC TTAGCGCTGG CCTTCCAGGA TGGCAGCGTG 
CACATGGTGC ACCGACTGTC CCTGCAGACC ATGGCGGTGT TCTACAGCTC AGCCCCGCGC 
TCGCTGGATG AGCCGGCCCT AAAGCGCCCA CGCACCACAT GCCCTGCCGT ACACTTTAAA 
GCCATGCAGC TGTCCTGGAC TTCGCTGGCC CTCGTGGGCA TCGATAACCA TGGGAAGCTC 
AGCATGCTGC GAATCTCTCC ATCCCTGGGC CACCCACTGG AGCCCAAGCT GGCCCTGCAG 
CACCTGCTTT TCCTGCTGGA ATACTGCATG GTGACCGGCT ATGACTGGTG GGACATCCTG 
CTACACGTGC AGCCCGGCAT GGTGCAGAGC CTGGTGGAGC GGCTGCATGA GGAGTACACT 
CGCCAGAAGC CCGCCCTGCA ACAGGTCCTC TCCACTCGGA TCCTGGCCAT GAAGGCTTCA 
CTGTGCAAGC TGTCACCCTG CACAGTGGCT CGTGTGTGTG ACTACCACAC CAAGCTGTTC 
CTCATGGCCA TCACGTCCAC CCTAAAGTCG CTGCTGCGCC CACACTTCCT CAACACCCCT 
GACAAGAGCC CTGGGGACCG CCTGGCCGAG ATCTGCGCCA AGATCACCGA TGTTGACATC 
GACAAAGTCA TGATCAACCT GAAGACTGAA GAGTTCGTCC TTGACATGAA CACTTTGCAG 
GCGCTGCAGC AGTTGCTACA GTGGGTGGGA GACTTCGTGC TCTACCTCCT GGTCAGCCTG 
CCCAACCAGG GCTCCCCGCT AAGGCCGGGC CACAGCTTCC TCCGAGATGG TACCTCCCTG 
GGCATGCTGC GAGAGTTGAT GGTTGTCATC CGAATCTGGG GCCTGCTGAA GCCCAGCTGT 
CTGCCCGTCT ACACAGCCAC CTCGGACACC CAGGACAGCA TGTCCCTGCT CTTCCGACTG 
CTCACCAAAC TGTGGATCTG CTGCCGTGAT GAGGGCGCAG CCAGTGAACC AGATGAGGGT 
TTGGTGGATG AATGCTGCCT GCTGCCTAGC CAGCTGCTGG TCCCCAACTT GGACTGGCTT 
CCCGCCAGTG ATGGCCTGGT CAGTCGCCTG CAGCCCAAAC AGCCCCTGCG CCTGCGCTTC 
GGCAGAGCGC CCACTCTGCC CAGCAGCACC TCCACCCTGC AGCTGGACGG CCTCACCAGG 
GCCCCTGGCC AGCCCAAGAT CGACCACCTC CGGAGGCTAC ACCTGGGCGC CTACCCCACC 
GAGGAGTGCA AAGCCTGCAC CAGGTGTGGC TGTGTCACCA TGCTGAAGTC TCCCAACAAG 
ACAACCGCTG TGACACAGTG GGAACAGCGG TGGATCAAGA ATTGCCTGTG TGGTGGGCTG 
TGGCGGCGAG TGCCTCTCAG CTGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med16 ( XM_006513488.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513551.1 Mus musculus mediator complex subunit 16 (Med16), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu44386
Clone ID Related Accession (Same CDS sequence)	XM_006513488.3 , XM_006513487.4 , XM_006513487.3 , XM_006513488.4
Accession Version	XM_006513488.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513488.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACC TGGCCTATGT GTGCGAATGG GAGAAGTGGG CCAAGAGCAC GTACTGCCCG 
TCGCTGCCCC TGGCTTGTGC CTGGTCCTGC AGGAACCTCA TTGCCTTCAC CACAGACCTA 
CGCAATGATG ACCAGGATCT GACACACATG ATCCATATCC TAGACACGGA ACACCCCTGG 
GAAGTGCACT CTGTCAGCTC TGGCCACAGC GAGGCCATCA CCTGCCTGGA GTGGGACCAG 
TCAGGGTCCC GGCTACTGTC TGCTGATGCT GATGGACAGA TCAAATGCTG GAGCATGGCT 
GACCACCTGG CCAACAGCTG GGAGAGCTCG GTGGGCAGCC AGGTGGAGGG GGACCCCATC 
GTGGCCCTGT CCTGGCTGCA CAACGGTGTA AAGCTGGCCC TGCATGTGGA GAAGTCTGGT 
GCCTCTAGCT TCGGAGAGAA GTTCTCACGT GTGAAGTTCT CCCCGTCCCT CACGCTGTTC 
GGTGGCAAGC CAATGGAGGG TTGGATTGCA GTGACAGTCA GTGGCCTGGT CACTGTGTCC 
CTTCTGAAGC CCAGCGGGCA GGTGCTGACG TCCACGGAGA GCCTGTGCCG GCTGCGTGGT 
AGAGTGGCGC TGGCTGACAT CGCCTTCACG GGCGGTGGGA ACATTGTGGT GGCTGCTGCA 
GATGGCAGCA GCGCGTCACC CGTGAAGTTC TATAAGGTGT GCGTCAGCGT GGTCAGCGAG 
AAGTGCCGCA TCGACACGGA GATCCTGCCC TCACTGTTTA TGCGCTGCAC CACCGACCCC 
AACCGCAAAG ACAGGTTCCC CGCCATCACA CACCTCAAGT TCCTGGCGCG AGACATGTCT 
GAGCAGGTGC TCCTGTGTGC GTCCAGCCAG ACCAGCAGCC TGGTGGAATG CTGGTCCCTT 
CGGAAAGAGG GCCTTCCTGT GAACAATATC TTCCAGCAGA TCTCGCCTGT GGTTGGTGAC 
AAACAGCCCA TGATCCTCAA GTGGCGAATC CTGTCGGCCA CCAATGACCT GGACCGTGTA 
TCCGCGGTGG CACTGCCGAA ACTGCCCATC TCACTCACCA ACACTGACCT CAAGGTGGCC 
AGTGACACCC AGTTCTATCC AGGCCTTGGC TTAGCGCTGG CCTTCCAGGA TGGCAGCGTG 
CACATGGTGC ACCGACTGTC CCTGCAGACC ATGGCGGTGT TCTACAGCTC AGCCCCGCGC 
TCGCTGGATG AGCCGGCCCT AAAGCGCCCA CGCACCACAT GCCCTGCCGT ACACTTTAAA 
GCCATGCAGC TGTCCTGGAC TTCGCTGGCC CTCGTGGGCA TCGATAACCA TGGGAAGCTC 
AGCATGCTGC GAATCTCTCC ATCCCTGGGC CACCCACTGG AGCCCAAGCT GGCCCTGCAG 
CACCTGCTTT TCCTGCTGGA ATACTGCATG GTGACCGGCT ATGACTGGTG GGACATCCTG 
CTACACGTGC AGCCCGGCAT GGTGCAGAGC CTGGTGGAGC GGCTGCATGA GGAGTACACT 
CGCCAGAAGC CCGCCCTGCA ACAGGTCCTC TCCACTCGGA TCCTGGCCAT GAAGGCTTCA 
CTGTGCAAGC TGTCACCCTG CACAGTGGCT CGTGTGTGTG ACTACCACAC CAAGCTGTTC 
CTCATGGCCA TCACGTCCAC CCTAAAGTCG CTGCTGCGCC CACACTTCCT CAACACCCCT 
GACAAGAGCC CTGGGGACCG CCTGGCCGAG ATCTGCGCCA AGATCACCGA TGTTGACATC 
GACAAAGTCA TGATCAACCT GAAGACTGAA GAGTTCGTCC TTGACATGAA CACTTTGCAG 
GCGCTGCAGC AGTTGCTACA GTGGGTGGGA GACTTCGTGC TCTACCTCCT GGTCAGCCTG 
CCCAACCAGG GCTCCCCGCT AAGGCCGGGC CACAGCTTCC TCCGAGATGG TACCTCCCTG 
GGCATGCTGC GAGAGTTGAT GGTTGTCATC CGAATCTGGG GCCTGCTGAA GCCCAGCTGT 
CTGCCCGTCT ACACAGCCAC CTCGGACACC CAGGACAGCA TGTCCCTGCT CTTCCGACTG 
CTCACCAAAC TGTGGATCTG CTGCCGTGAT GAGGGCGCAG CCAGTGAACC AGATGAGGGT 
TTGGTGGATG AATGCTGCCT GCTGCCTAGC CAGCTGCTGG TCCCCAACTT GGACTGGCTT 
CCCGCCAGTG ATGGCCTGGT CAGTCGCCTG CAGCCCAAAC AGCCCCTGCG CCTGCGCTTC 
GGCAGAGCGC CCACTCTGCC CAGCAGCACC TCCACCCTGC AGCTGGACGG CCTCACCAGG 
GCCCCTGGCC AGCCCAAGAT CGACCACCTC CGGAGGCTAC ACCTGGGCGC CTACCCCACC 
GAGGAGTGCA AAGCCTGCAC CAGGTGTGGC TGTGTCACCA TGCTGAAGTC TCCCAACAAG 
ACAACCGCTG TGACACAGTG GGAACAGCGG TGGATCAAGA ATTGCCTGTG TGGTGGGCTG 
TGGCGGCGAG TGCCTCTCAG CTGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513551.1
CDS	150..2639
Translation	MMDLAYVCEWEKWAKSTYCPSLPLACAWSCRNLIAFTTDLRNDDQDLTHMIHILDTEHPWEVHSVSSGHSEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWESSVGSQVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVAAADGSSASPVKFYKVCVSVVSEKCRIDTEILPSLFMRCTTDPNRKDRFPAITHLKFLARDMSEQVLLCASSQTSSLVECWSLRKEGLPVNNIFQQISPVVGDKQPMILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFQDGSVHMVHRLSLQTMAVFYSSAPRSLDEPALKRPRTTCPAVHFKAMQLSWTSLALVGIDNHGKLSMLRISPSLGHPLEPKLALQHLLFLLEYCMVTGYDWWDILLHVQPGMVQSLVERLHEEYTRQKPALQQVLSTRILAMKASLCKLSPCTVARVCDYHTKLFLMAITSTLKSLLRPHFLNTPDKSPGDRLAEICAKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLVSLPNQGSPLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICCRDEGAASEPDEGLVDECCLLPSQLLVPNLDWLPASDGLVSRLQPKQPLRLRFGRAPTLPSSTSTLQLDGLTRAPGQPKIDHLRRLHLGAYPTEECKACTRCGCVTMLKSPNKTTAVTQWEQRWIKNCLCGGLWRRVPLSCS

Target ORF information:

RefSeq Version	XM_006513488.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 16 (Med16), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513488.4

ORF Insert Sequence:

ATGATGGACC TGGCCTATGT GTGCGAATGG GAGAAGTGGG CCAAGAGCAC GTACTGCCCG 
TCGCTGCCCC TGGCTTGTGC CTGGTCCTGC AGGAACCTCA TTGCCTTCAC CACAGACCTA 
CGCAATGATG ACCAGGATCT GACACACATG ATCCATATCC TAGACACGGA ACACCCCTGG 
GAAGTGCACT CTGTCAGCTC TGGCCACAGC GAGGCCATCA CCTGCCTGGA GTGGGACCAG 
TCAGGGTCCC GGCTACTGTC TGCTGATGCT GATGGACAGA TCAAATGCTG GAGCATGGCT 
GACCACCTGG CCAACAGCTG GGAGAGCTCG GTGGGCAGCC AGGTGGAGGG GGACCCCATC 
GTGGCCCTGT CCTGGCTGCA CAACGGTGTA AAGCTGGCCC TGCATGTGGA GAAGTCTGGT 
GCCTCTAGCT TCGGAGAGAA GTTCTCACGT GTGAAGTTCT CCCCGTCCCT CACGCTGTTC 
GGTGGCAAGC CAATGGAGGG TTGGATTGCA GTGACAGTCA GTGGCCTGGT CACTGTGTCC 
CTTCTGAAGC CCAGCGGGCA GGTGCTGACG TCCACGGAGA GCCTGTGCCG GCTGCGTGGT 
AGAGTGGCGC TGGCTGACAT CGCCTTCACG GGCGGTGGGA ACATTGTGGT GGCTGCTGCA 
GATGGCAGCA GCGCGTCACC CGTGAAGTTC TATAAGGTGT GCGTCAGCGT GGTCAGCGAG 
AAGTGCCGCA TCGACACGGA GATCCTGCCC TCACTGTTTA TGCGCTGCAC CACCGACCCC 
AACCGCAAAG ACAGGTTCCC CGCCATCACA CACCTCAAGT TCCTGGCGCG AGACATGTCT 
GAGCAGGTGC TCCTGTGTGC GTCCAGCCAG ACCAGCAGCC TGGTGGAATG CTGGTCCCTT 
CGGAAAGAGG GCCTTCCTGT GAACAATATC TTCCAGCAGA TCTCGCCTGT GGTTGGTGAC 
AAACAGCCCA TGATCCTCAA GTGGCGAATC CTGTCGGCCA CCAATGACCT GGACCGTGTA 
TCCGCGGTGG CACTGCCGAA ACTGCCCATC TCACTCACCA ACACTGACCT CAAGGTGGCC 
AGTGACACCC AGTTCTATCC AGGCCTTGGC TTAGCGCTGG CCTTCCAGGA TGGCAGCGTG 
CACATGGTGC ACCGACTGTC CCTGCAGACC ATGGCGGTGT TCTACAGCTC AGCCCCGCGC 
TCGCTGGATG AGCCGGCCCT AAAGCGCCCA CGCACCACAT GCCCTGCCGT ACACTTTAAA 
GCCATGCAGC TGTCCTGGAC TTCGCTGGCC CTCGTGGGCA TCGATAACCA TGGGAAGCTC 
AGCATGCTGC GAATCTCTCC ATCCCTGGGC CACCCACTGG AGCCCAAGCT GGCCCTGCAG 
CACCTGCTTT TCCTGCTGGA ATACTGCATG GTGACCGGCT ATGACTGGTG GGACATCCTG 
CTACACGTGC AGCCCGGCAT GGTGCAGAGC CTGGTGGAGC GGCTGCATGA GGAGTACACT 
CGCCAGAAGC CCGCCCTGCA ACAGGTCCTC TCCACTCGGA TCCTGGCCAT GAAGGCTTCA 
CTGTGCAAGC TGTCACCCTG CACAGTGGCT CGTGTGTGTG ACTACCACAC CAAGCTGTTC 
CTCATGGCCA TCACGTCCAC CCTAAAGTCG CTGCTGCGCC CACACTTCCT CAACACCCCT 
GACAAGAGCC CTGGGGACCG CCTGGCCGAG ATCTGCGCCA AGATCACCGA TGTTGACATC 
GACAAAGTCA TGATCAACCT GAAGACTGAA GAGTTCGTCC TTGACATGAA CACTTTGCAG 
GCGCTGCAGC AGTTGCTACA GTGGGTGGGA GACTTCGTGC TCTACCTCCT GGTCAGCCTG 
CCCAACCAGG GCTCCCCGCT AAGGCCGGGC CACAGCTTCC TCCGAGATGG TACCTCCCTG 
GGCATGCTGC GAGAGTTGAT GGTTGTCATC CGAATCTGGG GCCTGCTGAA GCCCAGCTGT 
CTGCCCGTCT ACACAGCCAC CTCGGACACC CAGGACAGCA TGTCCCTGCT CTTCCGACTG 
CTCACCAAAC TGTGGATCTG CTGCCGTGAT GAGGGCGCAG CCAGTGAACC AGATGAGGGT 
TTGGTGGATG AATGCTGCCT GCTGCCTAGC CAGCTGCTGG TCCCCAACTT GGACTGGCTT 
CCCGCCAGTG ATGGCCTGGT CAGTCGCCTG CAGCCCAAAC AGCCCCTGCG CCTGCGCTTC 
GGCAGAGCGC CCACTCTGCC CAGCAGCACC TCCACCCTGC AGCTGGACGG CCTCACCAGG 
GCCCCTGGCC AGCCCAAGAT CGACCACCTC CGGAGGCTAC ACCTGGGCGC CTACCCCACC 
GAGGAGTGCA AAGCCTGCAC CAGGTGTGGC TGTGTCACCA TGCTGAAGTC TCCCAACAAG 
ACAACCGCTG TGACACAGTG GGAACAGCGG TGGATCAAGA ATTGCCTGTG TGGTGGGCTG 
TGGCGGCGAG TGCCTCTCAG CTGTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med16 ( NM_198107.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_932775.2 Mus musculus mediator complex subunit 16 (Med16), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08693
Clone ID Related Accession (Same CDS sequence)	NM_198107.2
Accession Version	NM_198107.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 16 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY019373.1, AK042340.1, DT908554.1, AK149345.1 and BB731333.1. On Jul 15, 2009 this sequence version replaced NM_198107.1. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: BC057056.1, SRR1660817.229923.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGT CGGATCCCGG GCGTTGGGGC GAGTGTGGAC CGCGGCCGCC GGGCCGACTG 
GGCGCCCGGG GCGGACAGGA GCCAGGGACC GTGCTGGGTG CGGTGGGTAT GATGGACCTG 
GCCTATGTGT GCGAATGGGA GAAGTGGGCC AAGAGCACGT ACTGCCCGTC GCTGCCCCTG 
GCTTGTGCCT GGTCCTGCAG GAACCTCATT GCCTTCACCA CAGACCTACG CAATGATGAC 
CAGGATCTGA CACACATGAT CCATATCCTA GACACGGAAC ACCCCTGGGA AGTGCACTCT 
GTCAGCTCTG GCCACAGCGA GGCCATCACC TGCCTGGAGT GGGACCAGTC AGGGTCCCGG 
CTACTGTCTG CTGATGCTGA TGGACAGATC AAATGCTGGA GCATGGCTGA CCACCTGGCC 
AACAGCTGGG AGAGCTCGGT GGGCAGCCAG GTGGAGGGGG ACCCCATCGT GGCCCTGTCC 
TGGCTGCACA ACGGTGTAAA GCTGGCCCTG CATGTGGAGA AGTCTGGTGC CTCTAGCTTC 
GGAGAGAAGT TCTCACGTGT GAAGTTCTCC CCGTCCCTCA CGCTGTTCGG TGGCAAGCCA 
ATGGAGGGTT GGATTGCAGT GACAGTCAGT GGCCTGGTCA CTGTGTCCCT TCTGAAGCCC 
AGCGGGCAGG TGCTGACGTC CACGGAGAGC CTGTGCCGGC TGCGTGGTAG AGTGGCGCTG 
GCTGACATCG CCTTCACGGG CGGTGGGAAC ATTGTGGTGG CTGCTGCAGA TGGCAGCAGC 
GCGTCACCCG TGAAGTTCTA TAAGGTGTGC GTCAGCGTGG TCAGCGAGAA GTGCCGCATC 
GACACGGAGA TCCTGCCCTC ACTGTTTATG CGCTGCACCA CCGACCCCAA CCGCAAAGAC 
AGGTTCCCCG CCATCACACA CCTCAAGTTC CTGGCGCGAG ACATGTCTGA GCAGGTGCTC 
CTGTGTGCGT CCAGCCAGAC CAGCAGCCTG GTGGAATGCT GGTCCCTTCG GAAAGAGGGC 
CTTCCTGTGA ACAATATCTT CCAGCAGATC TCGCCTGTGG TTGGTGACAA ACAGCCCATG 
ATCCTCAAGT GGCGAATCCT GTCGGCCACC AATGACCTGG ACCGTGTATC CGCGGTGGCA 
CTGCCGAAAC TGCCCATCTC ACTCACCAAC ACTGACCTCA AGGTGGCCAG TGACACCCAG 
TTCTATCCAG GCCTTGGCTT AGCGCTGGCC TTCCAGGATG GCAGCGTGCA CATGGTGCAC 
CGACTGTCCC TGCAGACCAT GGCGGTGTTC TACAGCTCAG CCCCGCGCTC GCTGGATGAG 
CCGGCCCTAA AGCGCCCACG CACCACATGC CCTGCCGTAC ACTTTAAAGC CATGCAGCTG 
TCCTGGACTT CGCTGGCCCT CGTGGGCATC GATAACCATG GGAAGCTCAG CATGCTGCGA 
ATCTCTCCAT CCCTGGGCCA CCCACTGGAG CCCAAGCTGG CCCTGCAGCA CCTGCTTTTC 
CTGCTGGAAT ACTGCATGGT GACCGGCTAT GACTGGTGGG ACATCCTGCT ACACGTGCAG 
CCCGGCATGG TGCAGAGCCT GGTGGAGCGG CTGCATGAGG AGTACACTCG CCAGAAGCCC 
GCCCTGCAAC AGGTCCTCTC CACTCGGATC CTGGCCATGA AGGCTTCACT GTGCAAGCTG 
TCACCCTGCA CAGTGGCTCG TGTGTGTGAC TACCACACCA AGCTGTTCCT CATGGCCATC 
ACGTCCACCC TAAAGTCGCT GCTGCGCCCA CACTTCCTCA ACACCCCTGA CAAGAGCCCT 
GGGGACCGCC TGGCCGAGAT CTGCGCCAAG ATCACCGATG TTGACATCGA CAAAGTCATG 
ATCAACCTGA AGACTGAAGA GTTCGTCCTT GACATGAACA CTTTGCAGGC GCTGCAGCAG 
TTGCTACAGT GGGTGGGAGA CTTCGTGCTC TACCTCCTGG TCAGCCTGCC CAACCAGGGC 
TCCCCGCTAA GGCCGGGCCA CAGCTTCCTC CGAGATGGTA CCTCCCTGGG CATGCTGCGA 
GAGTTGATGG TTGTCATCCG AATCTGGGGC CTGCTGAAGC CCAGCTGTCT GCCCGTCTAC 
ACAGCCACCT CGGACACCCA GGACAGCATG TCCCTGCTCT TCCGACTGCT CACCAAACTG 
TGGATCTGCT GCCGTGATGA GGGCGCAGCC AGTGAACCAG ATGAGGGTTT GGTGGATGAA 
TGCTGCCTGC TGCCTAGCCA GCTGCTGGTC CCCAACTTGG ACTGGCTTCC CGCCAGTGAT 
GGCCTGGTCA GTCGCCTGCA GCCCAAACAG CCCCTGCGCC TGCGCTTCGG CAGAGCGCCC 
ACTCTGCCCA GCAGCACCTC CACCCTGCAG CTGGACGGCC TCACCAGGGC CCCTGGCCAG 
CCCAAGATCG ACCACCTCCG GAGGCTACAC CTGGGCGCCT ACCCCACCGA GGAGTGCAAA 
GCCTGCACCA GGTGTGGCTG TGTCACCATG CTGAAGTCTC CCAACAAGAC AACCGCTGTG 
ACACAGTGGG AACAGCGGTG GATCAAGAAT TGCCTGTGTG GTGGGCTGTG GCGGCGAGTG 
CCTCTCAGCT GTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_932775.2
CDS	44..2641
Translation	MAASDPGRWGECGPRPPGRLGARGGQEPGTVLGAVGMMDLAYVCEWEKWAKSTYCPSLPLACAWSCRNLIAFTTDLRNDDQDLTHMIHILDTEHPWEVHSVSSGHSEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWESSVGSQVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVAAADGSSASPVKFYKVCVSVVSEKCRIDTEILPSLFMRCTTDPNRKDRFPAITHLKFLARDMSEQVLLCASSQTSSLVECWSLRKEGLPVNNIFQQISPVVGDKQPMILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFQDGSVHMVHRLSLQTMAVFYSSAPRSLDEPALKRPRTTCPAVHFKAMQLSWTSLALVGIDNHGKLSMLRISPSLGHPLEPKLALQHLLFLLEYCMVTGYDWWDILLHVQPGMVQSLVERLHEEYTRQKPALQQVLSTRILAMKASLCKLSPCTVARVCDYHTKLFLMAITSTLKSLLRPHFLNTPDKSPGDRLAEICAKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLVSLPNQGSPLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICCRDEGAASEPDEGLVDECCLLPSQLLVPNLDWLPASDGLVSRLQPKQPLRLRFGRAPTLPSSTSTLQLDGLTRAPGQPKIDHLRRLHLGAYPTEECKACTRCGCVTMLKSPNKTTAVTQWEQRWIKNCLCGGLWRRVPLSCS

Target ORF information:

RefSeq Version	NM_198107.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 16 (Med16), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198107.2

ORF Insert Sequence:

ATGGCGGCGT CGGATCCCGG GCGTTGGGGC GAGTGTGGAC CGCGGCCGCC GGGCCGACTG 
GGCGCCCGGG GCGGACAGGA GCCAGGGACC GTGCTGGGTG CGGTGGGTAT GATGGACCTG 
GCCTATGTGT GCGAATGGGA GAAGTGGGCC AAGAGCACGT ACTGCCCGTC GCTGCCCCTG 
GCTTGTGCCT GGTCCTGCAG GAACCTCATT GCCTTCACCA CAGACCTACG CAATGATGAC 
CAGGATCTGA CACACATGAT CCATATCCTA GACACGGAAC ACCCCTGGGA AGTGCACTCT 
GTCAGCTCTG GCCACAGCGA GGCCATCACC TGCCTGGAGT GGGACCAGTC AGGGTCCCGG 
CTACTGTCTG CTGATGCTGA TGGACAGATC AAATGCTGGA GCATGGCTGA CCACCTGGCC 
AACAGCTGGG AGAGCTCGGT GGGCAGCCAG GTGGAGGGGG ACCCCATCGT GGCCCTGTCC 
TGGCTGCACA ACGGTGTAAA GCTGGCCCTG CATGTGGAGA AGTCTGGTGC CTCTAGCTTC 
GGAGAGAAGT TCTCACGTGT GAAGTTCTCC CCGTCCCTCA CGCTGTTCGG TGGCAAGCCA 
ATGGAGGGTT GGATTGCAGT GACAGTCAGT GGCCTGGTCA CTGTGTCCCT TCTGAAGCCC 
AGCGGGCAGG TGCTGACGTC CACGGAGAGC CTGTGCCGGC TGCGTGGTAG AGTGGCGCTG 
GCTGACATCG CCTTCACGGG CGGTGGGAAC ATTGTGGTGG CTGCTGCAGA TGGCAGCAGC 
GCGTCACCCG TGAAGTTCTA TAAGGTGTGC GTCAGCGTGG TCAGCGAGAA GTGCCGCATC 
GACACGGAGA TCCTGCCCTC ACTGTTTATG CGCTGCACCA CCGACCCCAA CCGCAAAGAC 
AGGTTCCCCG CCATCACACA CCTCAAGTTC CTGGCGCGAG ACATGTCTGA GCAGGTGCTC 
CTGTGTGCGT CCAGCCAGAC CAGCAGCCTG GTGGAATGCT GGTCCCTTCG GAAAGAGGGC 
CTTCCTGTGA ACAATATCTT CCAGCAGATC TCGCCTGTGG TTGGTGACAA ACAGCCCATG 
ATCCTCAAGT GGCGAATCCT GTCGGCCACC AATGACCTGG ACCGTGTATC CGCGGTGGCA 
CTGCCGAAAC TGCCCATCTC ACTCACCAAC ACTGACCTCA AGGTGGCCAG TGACACCCAG 
TTCTATCCAG GCCTTGGCTT AGCGCTGGCC TTCCAGGATG GCAGCGTGCA CATGGTGCAC 
CGACTGTCCC TGCAGACCAT GGCGGTGTTC TACAGCTCAG CCCCGCGCTC GCTGGATGAG 
CCGGCCCTAA AGCGCCCACG CACCACATGC CCTGCCGTAC ACTTTAAAGC CATGCAGCTG 
TCCTGGACTT CGCTGGCCCT CGTGGGCATC GATAACCATG GGAAGCTCAG CATGCTGCGA 
ATCTCTCCAT CCCTGGGCCA CCCACTGGAG CCCAAGCTGG CCCTGCAGCA CCTGCTTTTC 
CTGCTGGAAT ACTGCATGGT GACCGGCTAT GACTGGTGGG ACATCCTGCT ACACGTGCAG 
CCCGGCATGG TGCAGAGCCT GGTGGAGCGG CTGCATGAGG AGTACACTCG CCAGAAGCCC 
GCCCTGCAAC AGGTCCTCTC CACTCGGATC CTGGCCATGA AGGCTTCACT GTGCAAGCTG 
TCACCCTGCA CAGTGGCTCG TGTGTGTGAC TACCACACCA AGCTGTTCCT CATGGCCATC 
ACGTCCACCC TAAAGTCGCT GCTGCGCCCA CACTTCCTCA ACACCCCTGA CAAGAGCCCT 
GGGGACCGCC TGGCCGAGAT CTGCGCCAAG ATCACCGATG TTGACATCGA CAAAGTCATG 
ATCAACCTGA AGACTGAAGA GTTCGTCCTT GACATGAACA CTTTGCAGGC GCTGCAGCAG 
TTGCTACAGT GGGTGGGAGA CTTCGTGCTC TACCTCCTGG TCAGCCTGCC CAACCAGGGC 
TCCCCGCTAA GGCCGGGCCA CAGCTTCCTC CGAGATGGTA CCTCCCTGGG CATGCTGCGA 
GAGTTGATGG TTGTCATCCG AATCTGGGGC CTGCTGAAGC CCAGCTGTCT GCCCGTCTAC 
ACAGCCACCT CGGACACCCA GGACAGCATG TCCCTGCTCT TCCGACTGCT CACCAAACTG 
TGGATCTGCT GCCGTGATGA GGGCGCAGCC AGTGAACCAG ATGAGGGTTT GGTGGATGAA 
TGCTGCCTGC TGCCTAGCCA GCTGCTGGTC CCCAACTTGG ACTGGCTTCC CGCCAGTGAT 
GGCCTGGTCA GTCGCCTGCA GCCCAAACAG CCCCTGCGCC TGCGCTTCGG CAGAGCGCCC 
ACTCTGCCCA GCAGCACCTC CACCCTGCAG CTGGACGGCC TCACCAGGGC CCCTGGCCAG 
CCCAAGATCG ACCACCTCCG GAGGCTACAC CTGGGCGCCT ACCCCACCGA GGAGTGCAAA 
GCCTGCACCA GGTGTGGCTG TGTCACCATG CTGAAGTCTC CCAACAAGAC AACCGCTGTG 
ACACAGTGGG AACAGCGGTG GATCAAGAAT TGCCTGTGTG GTGGGCTGTG GCGGCGAGTG 
CCTCTCAGCT GTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med16 ( NM_001163276.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156748.1 Mus musculus mediator complex subunit 16 (Med16), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu08852
Clone ID Related Accession (Same CDS sequence)	NM_001163276.1
Accession Version	NM_001163276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 16 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY019373.1, AK149345.1, BY480428.1 and BB731333.1. Transcript Variant: This variant (2) uses a different splice site in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter by 1 aa when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK149345.1, SRR1660811.42318.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGT CGGATCCCGG GCGTTGGGGC GAGTGTGGAC CGCGGCCGCC GGGCCGACTG 
GGCGCCCGGG GCGGACAGCC AGGGACCGTG CTGGGTGCGG TGGGTATGAT GGACCTGGCC 
TATGTGTGCG AATGGGAGAA GTGGGCCAAG AGCACGTACT GCCCGTCGCT GCCCCTGGCT 
TGTGCCTGGT CCTGCAGGAA CCTCATTGCC TTCACCACAG ACCTACGCAA TGATGACCAG 
GATCTGACAC ACATGATCCA TATCCTAGAC ACGGAACACC CCTGGGAAGT GCACTCTGTC 
AGCTCTGGCC ACAGCGAGGC CATCACCTGC CTGGAGTGGG ACCAGTCAGG GTCCCGGCTA 
CTGTCTGCTG ATGCTGATGG ACAGATCAAA TGCTGGAGCA TGGCTGACCA CCTGGCCAAC 
AGCTGGGAGA GCTCGGTGGG CAGCCAGGTG GAGGGGGACC CCATCGTGGC CCTGTCCTGG 
CTGCACAACG GTGTAAAGCT GGCCCTGCAT GTGGAGAAGT CTGGTGCCTC TAGCTTCGGA 
GAGAAGTTCT CACGTGTGAA GTTCTCCCCG TCCCTCACGC TGTTCGGTGG CAAGCCAATG 
GAGGGTTGGA TTGCAGTGAC AGTCAGTGGC CTGGTCACTG TGTCCCTTCT GAAGCCCAGC 
GGGCAGGTGC TGACGTCCAC GGAGAGCCTG TGCCGGCTGC GTGGTAGAGT GGCGCTGGCT 
GACATCGCCT TCACGGGCGG TGGGAACATT GTGGTGGCTG CTGCAGATGG CAGCAGCGCG 
TCACCCGTGA AGTTCTATAA GGTGTGCGTC AGCGTGGTCA GCGAGAAGTG CCGCATCGAC 
ACGGAGATCC TGCCCTCACT GTTTATGCGC TGCACCACCG ACCCCAACCG CAAAGACAGG 
TTCCCCGCCA TCACACACCT CAAGTTCCTG GCGCGAGACA TGTCTGAGCA GGTGCTCCTG 
TGTGCGTCCA GCCAGACCAG CAGCCTGGTG GAATGCTGGT CCCTTCGGAA AGAGGGCCTT 
CCTGTGAACA ATATCTTCCA GCAGATCTCG CCTGTGGTTG GTGACAAACA GCCCATGATC 
CTCAAGTGGC GAATCCTGTC GGCCACCAAT GACCTGGACC GTGTATCCGC GGTGGCACTG 
CCGAAACTGC CCATCTCACT CACCAACACT GACCTCAAGG TGGCCAGTGA CACCCAGTTC 
TATCCAGGCC TTGGCTTAGC GCTGGCCTTC CAGGATGGCA GCGTGCACAT GGTGCACCGA 
CTGTCCCTGC AGACCATGGC GGTGTTCTAC AGCTCAGCCC CGCGCTCGCT GGATGAGCCG 
GCCCTAAAGC GCCCACGCAC CACATGCCCT GCCGTACACT TTAAAGCCAT GCAGCTGTCC 
TGGACTTCGC TGGCCCTCGT GGGCATCGAT AACCATGGGA AGCTCAGCAT GCTGCGAATC 
TCTCCATCCC TGGGCCACCC ACTGGAGCCC AAGCTGGCCC TGCAGCACCT GCTTTTCCTG 
CTGGAATACT GCATGGTGAC CGGCTATGAC TGGTGGGACA TCCTGCTACA CGTGCAGCCC 
GGCATGGTGC AGAGCCTGGT GGAGCGGCTG CATGAGGAGT ACACTCGCCA GAAGCCCGCC 
CTGCAACAGG TCCTCTCCAC TCGGATCCTG GCCATGAAGG CTTCACTGTG CAAGCTGTCA 
CCCTGCACAG TGGCTCGTGT GTGTGACTAC CACACCAAGC TGTTCCTCAT GGCCATCACG 
TCCACCCTAA AGTCGCTGCT GCGCCCACAC TTCCTCAACA CCCCTGACAA GAGCCCTGGG 
GACCGCCTGG CCGAGATCTG CGCCAAGATC ACCGATGTTG ACATCGACAA AGTCATGATC 
AACCTGAAGA CTGAAGAGTT CGTCCTTGAC ATGAACACTT TGCAGGCGCT GCAGCAGTTG 
CTACAGTGGG TGGGAGACTT CGTGCTCTAC CTCCTGGTCA GCCTGCCCAA CCAGGGCTCC 
CCGCTAAGGC CGGGCCACAG CTTCCTCCGA GATGGTACCT CCCTGGGCAT GCTGCGAGAG 
TTGATGGTTG TCATCCGAAT CTGGGGCCTG CTGAAGCCCA GCTGTCTGCC CGTCTACACA 
GCCACCTCGG ACACCCAGGA CAGCATGTCC CTGCTCTTCC GACTGCTCAC CAAACTGTGG 
ATCTGCTGCC GTGATGAGGG CGCAGCCAGT GAACCAGATG AGGGTTTGGT GGATGAATGC 
TGCCTGCTGC CTAGCCAGCT GCTGGTCCCC AACTTGGACT GGCTTCCCGC CAGTGATGGC 
CTGGTCAGTC GCCTGCAGCC CAAACAGCCC CTGCGCCTGC GCTTCGGCAG AGCGCCCACT 
CTGCCCAGCA GCACCTCCAC CCTGCAGCTG GACGGCCTCA CCAGGGCCCC TGGCCAGCCC 
AAGATCGACC ACCTCCGGAG GCTACACCTG GGCGCCTACC CCACCGAGGA GTGCAAAGCC 
TGCACCAGGT GTGGCTGTGT CACCATGCTG AAGTCTCCCA ACAAGACAAC CGCTGTGACA 
CAGTGGGAAC AGCGGTGGAT CAAGAATTGC CTGTGTGGTG GGCTGTGGCG GCGAGTGCCT 
CTCAGCTGTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156748.1
CDS	44..2638
Translation	MAASDPGRWGECGPRPPGRLGARGGQPGTVLGAVGMMDLAYVCEWEKWAKSTYCPSLPLACAWSCRNLIAFTTDLRNDDQDLTHMIHILDTEHPWEVHSVSSGHSEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWESSVGSQVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVAAADGSSASPVKFYKVCVSVVSEKCRIDTEILPSLFMRCTTDPNRKDRFPAITHLKFLARDMSEQVLLCASSQTSSLVECWSLRKEGLPVNNIFQQISPVVGDKQPMILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFQDGSVHMVHRLSLQTMAVFYSSAPRSLDEPALKRPRTTCPAVHFKAMQLSWTSLALVGIDNHGKLSMLRISPSLGHPLEPKLALQHLLFLLEYCMVTGYDWWDILLHVQPGMVQSLVERLHEEYTRQKPALQQVLSTRILAMKASLCKLSPCTVARVCDYHTKLFLMAITSTLKSLLRPHFLNTPDKSPGDRLAEICAKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLVSLPNQGSPLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICCRDEGAASEPDEGLVDECCLLPSQLLVPNLDWLPASDGLVSRLQPKQPLRLRFGRAPTLPSSTSTLQLDGLTRAPGQPKIDHLRRLHLGAYPTEECKACTRCGCVTMLKSPNKTTAVTQWEQRWIKNCLCGGLWRRVPLSCS

Target ORF information:

RefSeq Version	NM_001163276.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 16 (Med16), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163276.1

ORF Insert Sequence:

ATGGCGGCGT CGGATCCCGG GCGTTGGGGC GAGTGTGGAC CGCGGCCGCC GGGCCGACTG 
GGCGCCCGGG GCGGACAGCC AGGGACCGTG CTGGGTGCGG TGGGTATGAT GGACCTGGCC 
TATGTGTGCG AATGGGAGAA GTGGGCCAAG AGCACGTACT GCCCGTCGCT GCCCCTGGCT 
TGTGCCTGGT CCTGCAGGAA CCTCATTGCC TTCACCACAG ACCTACGCAA TGATGACCAG 
GATCTGACAC ACATGATCCA TATCCTAGAC ACGGAACACC CCTGGGAAGT GCACTCTGTC 
AGCTCTGGCC ACAGCGAGGC CATCACCTGC CTGGAGTGGG ACCAGTCAGG GTCCCGGCTA 
CTGTCTGCTG ATGCTGATGG ACAGATCAAA TGCTGGAGCA TGGCTGACCA CCTGGCCAAC 
AGCTGGGAGA GCTCGGTGGG CAGCCAGGTG GAGGGGGACC CCATCGTGGC CCTGTCCTGG 
CTGCACAACG GTGTAAAGCT GGCCCTGCAT GTGGAGAAGT CTGGTGCCTC TAGCTTCGGA 
GAGAAGTTCT CACGTGTGAA GTTCTCCCCG TCCCTCACGC TGTTCGGTGG CAAGCCAATG 
GAGGGTTGGA TTGCAGTGAC AGTCAGTGGC CTGGTCACTG TGTCCCTTCT GAAGCCCAGC 
GGGCAGGTGC TGACGTCCAC GGAGAGCCTG TGCCGGCTGC GTGGTAGAGT GGCGCTGGCT 
GACATCGCCT TCACGGGCGG TGGGAACATT GTGGTGGCTG CTGCAGATGG CAGCAGCGCG 
TCACCCGTGA AGTTCTATAA GGTGTGCGTC AGCGTGGTCA GCGAGAAGTG CCGCATCGAC 
ACGGAGATCC TGCCCTCACT GTTTATGCGC TGCACCACCG ACCCCAACCG CAAAGACAGG 
TTCCCCGCCA TCACACACCT CAAGTTCCTG GCGCGAGACA TGTCTGAGCA GGTGCTCCTG 
TGTGCGTCCA GCCAGACCAG CAGCCTGGTG GAATGCTGGT CCCTTCGGAA AGAGGGCCTT 
CCTGTGAACA ATATCTTCCA GCAGATCTCG CCTGTGGTTG GTGACAAACA GCCCATGATC 
CTCAAGTGGC GAATCCTGTC GGCCACCAAT GACCTGGACC GTGTATCCGC GGTGGCACTG 
CCGAAACTGC CCATCTCACT CACCAACACT GACCTCAAGG TGGCCAGTGA CACCCAGTTC 
TATCCAGGCC TTGGCTTAGC GCTGGCCTTC CAGGATGGCA GCGTGCACAT GGTGCACCGA 
CTGTCCCTGC AGACCATGGC GGTGTTCTAC AGCTCAGCCC CGCGCTCGCT GGATGAGCCG 
GCCCTAAAGC GCCCACGCAC CACATGCCCT GCCGTACACT TTAAAGCCAT GCAGCTGTCC 
TGGACTTCGC TGGCCCTCGT GGGCATCGAT AACCATGGGA AGCTCAGCAT GCTGCGAATC 
TCTCCATCCC TGGGCCACCC ACTGGAGCCC AAGCTGGCCC TGCAGCACCT GCTTTTCCTG 
CTGGAATACT GCATGGTGAC CGGCTATGAC TGGTGGGACA TCCTGCTACA CGTGCAGCCC 
GGCATGGTGC AGAGCCTGGT GGAGCGGCTG CATGAGGAGT ACACTCGCCA GAAGCCCGCC 
CTGCAACAGG TCCTCTCCAC TCGGATCCTG GCCATGAAGG CTTCACTGTG CAAGCTGTCA 
CCCTGCACAG TGGCTCGTGT GTGTGACTAC CACACCAAGC TGTTCCTCAT GGCCATCACG 
TCCACCCTAA AGTCGCTGCT GCGCCCACAC TTCCTCAACA CCCCTGACAA GAGCCCTGGG 
GACCGCCTGG CCGAGATCTG CGCCAAGATC ACCGATGTTG ACATCGACAA AGTCATGATC 
AACCTGAAGA CTGAAGAGTT CGTCCTTGAC ATGAACACTT TGCAGGCGCT GCAGCAGTTG 
CTACAGTGGG TGGGAGACTT CGTGCTCTAC CTCCTGGTCA GCCTGCCCAA CCAGGGCTCC 
CCGCTAAGGC CGGGCCACAG CTTCCTCCGA GATGGTACCT CCCTGGGCAT GCTGCGAGAG 
TTGATGGTTG TCATCCGAAT CTGGGGCCTG CTGAAGCCCA GCTGTCTGCC CGTCTACACA 
GCCACCTCGG ACACCCAGGA CAGCATGTCC CTGCTCTTCC GACTGCTCAC CAAACTGTGG 
ATCTGCTGCC GTGATGAGGG CGCAGCCAGT GAACCAGATG AGGGTTTGGT GGATGAATGC 
TGCCTGCTGC CTAGCCAGCT GCTGGTCCCC AACTTGGACT GGCTTCCCGC CAGTGATGGC 
CTGGTCAGTC GCCTGCAGCC CAAACAGCCC CTGCGCCTGC GCTTCGGCAG AGCGCCCACT 
CTGCCCAGCA GCACCTCCAC CCTGCAGCTG GACGGCCTCA CCAGGGCCCC TGGCCAGCCC 
AAGATCGACC ACCTCCGGAG GCTACACCTG GGCGCCTACC CCACCGAGGA GTGCAAAGCC 
TGCACCAGGT GTGGCTGTGT CACCATGCTG AAGTCTCCCA ACAAGACAAC CGCTGTGACA 
CAGTGGGAAC AGCGGTGGAT CAAGAATTGC CTGTGTGGTG GGCTGTGGCG GCGAGTGCCT 
CTCAGCTGTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med16 ( NM_001359629.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346558.1 Mus musculus mediator complex subunit 16 (Med16), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu72948
Clone ID Related Accession (Same CDS sequence)	NM_001359629.1 , XM_011243402.2
Accession Version	NM_001359629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 16 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151846.3, BY133011.1, AK042340.1, BC057056.1 and CR520085.1. On Jan 13, 2018 this sequence version replaced XM_011243402.2. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting protein (isoform 3), has a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.121252.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGT CGGATCCCGG GCGTTGGGGC GAGTGTGGAC CGCGGCCGCC GGGCCGACTG 
GGCGCCCGGG GCGGACAGGA GCCAGGGACC GTGCTGGGTG CGGTGGGTAT GATGGACCTG 
GCCTATGTGT GCGAATGGGA GAAGTGGGCC AAGAGCACGT ACTGCCCGTC GCTGCCCCTG 
GCTTGTGCCT GGTCCTGCAG GAACCTCATT GCCTTCACCA CAGACCTACG CAATGATGAC 
CAGGATCTGA CACACATGAT CCATATCCTA GACACGGAAC ACCCCTGGGA AGTGCACTCT 
GTCAGCTCTG GCCACAGCGA GGCCATCACC TGCCTGGAGT GGGACCAGTC AGGGTCCCGG 
CTACTGTCTG CTGATGCTGA TGGACAGATC AAATGCTGGA GCATGGCTGA CCACCTGGCC 
AACAGCTGGG AGAGCTCGGT GGGCAGCCAG GTGGAGGGGG ACCCCATCGT GGCCCTGTCC 
TGGCTGCACA ACGGTGTAAA GCTGGCCCTG CATGTGGAGA AGTCTGGTGC CTCTAGCTTC 
GGAGAGAAGT TCTCACGTGT GAAGTTCTCC CCGTCCCTCA CGCTGTTCGG TGGCAAGCCA 
ATGGAGGGTT GGATTGCAGT GACAGTCAGT GGCCTGGTCA CTGTGTCCCT TCTGAAGCCC 
AGCGGGCAGG TGCTGACGTC CACGGAGAGC CTGTGCCGGC TGCGTGGTAG AGTGGCGCTG 
GCTGACATCG CCTTCACGGG CGGTGGGAAC ATTGTGGTGG CTGCTGCAGA TGGCAGCAGC 
GCGTCACCCG TGAAGTTCTA TAAGGTGTGC GTCAGCGTGG TCAGCGAGAA GTGCCGCATC 
GACACGGAGA TCCTGCCCTC ACTGTTTATG CGCTGCACCA CCGACCCCAA CCGCAAAGAC 
AGGTTCCCCG CCATCACACA CCTCAAGTTC CTGGCGCGAG ACATGTCTGA GCAGGTGCTC 
CTGTGTGCGT CCAGCCAGAC CAGCAGCCTG GTGGAATGCT GGTCCCTTCG GAAAGAGGGC 
CTTCCTGTGA ACAATATCTT CCAGCAGATC TCGCCTGTGG TTGGTGACAA ACAGCCCATG 
ATCCTCAAGT GGCGAATCCT GTCGGCCACC AATGACCTGG ACCGTGTATC CGCGGTGGCA 
CTGCCGAAAC TGCCCATCTC ACTCACCAAC ACTGACCTCA AGGTGGCCAG TGACACCCAG 
TTCTATCCAG GCCTTGGCTT AGCGCTGGCC TTCCAGGATG GCAGCGTGCA CATGGTGCAC 
CGACTGTCCC TGCAGACCAT GGCGGTGTTC TACAGCTCAG CCCCGCGCTC GCTGGATGAG 
CCGGCCCTAA AGCGCCCACG CACCACATGC CCTGCCGTAC ACTTTAAAGC CATGCAGCTG 
TCCTGGACTT CGCTGGCCCT CGTGGGCATC GATAACCATG GGAAGCTCAG CATGCTGCGA 
ATCTCTCCAT CCCTGGGCCA CCCACTGGAG CCCAAGCTGG CCCTGCAGCA CCTGCTTTTC 
CTGCTGGAAT ACTGCATGGT GACCGGCTAT GACTGGTGGG ACATCCTGCT ACACGTGCAG 
CCCGGCATGG TGCAGAGCCT GGTGGAGCGG CTGCATGAGG AGTACACTCG CCAGAAGCCC 
GCCCTGCAAC AGGTCCTCTC CACTCGGATC CTGGCCATGA AGGCTTCACT GTGCAAGCTG 
TCACCCTGCA CAGTGGCTCG TGTGTGTGAC TACCACACCA AGCTGTTCCT CATGGCCATC 
ACGTCCACCC TAAAGTCGCT GCTGCGCCCA CACTTCCTCA ACACCCCTGA CAAGAGCCCT 
GGGGACCGCC TGGCCGAGAT CTGCGCCAAG ATCACCGATG TTGACATCGA CAAAGTCATG 
ATCAACCTGA AGACTGAAGA GTTCGTCCTT GACATGAACA CTTTGCAGGC GCTGCAGCAG 
TTGCTACAGT GGGTGGGAGA CTTCGTGCTC TACCTCCTGG TCAGCCTGCC CAACCAGGGC 
TCCCCGCTAA GGCCGGGCCA CAGCTTCCTC CGAGATGGTA CCTCCCTGGG CATGCTGCGA 
GAGTTGATGG TTGTCATCCG AATCTGGGGC CTGCTGAAGC CCAGCTGTCT GCCCGTCTAC 
ACAGCCACCT CGGACACCCA GGACAGCATG TCCCTGCTCT TCCGACTGCT CACCAAACTG 
TGGATCTGCT GTGAGGCCGT GATGAGGGCG CAGCCAGTGA ACCAGATGAG GGTTTGGTGG 
ATGAATGCTG CCTGCTGCCT AGCCAGCTGC TGGTCCCCAA CTTGGACTGG CTTCCCGCCA 
GTGATGGCCT GGTCAGTCGC CTGCAGCCCA AACAGCCCCT GCGCCTGCGC TTCGGCAGAG 
CGCCCACTCT GCCCAGCAGC ACCTCCACCC TGCAGCTGGA CGGCCTCACC AGGGCCCCTG 
GCCAGCCCAA GATCGACCAC CTCCGGAGGC TACACCTGGG CGCCTACCCC ACCGAGGAGT 
GCAAAGCCTG CACCAGGTGT GGCTGTGTCA CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346558.1
CDS	44..2542
Translation	MAASDPGRWGECGPRPPGRLGARGGQEPGTVLGAVGMMDLAYVCEWEKWAKSTYCPSLPLACAWSCRNLIAFTTDLRNDDQDLTHMIHILDTEHPWEVHSVSSGHSEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWESSVGSQVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVAAADGSSASPVKFYKVCVSVVSEKCRIDTEILPSLFMRCTTDPNRKDRFPAITHLKFLARDMSEQVLLCASSQTSSLVECWSLRKEGLPVNNIFQQISPVVGDKQPMILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFQDGSVHMVHRLSLQTMAVFYSSAPRSLDEPALKRPRTTCPAVHFKAMQLSWTSLALVGIDNHGKLSMLRISPSLGHPLEPKLALQHLLFLLEYCMVTGYDWWDILLHVQPGMVQSLVERLHEEYTRQKPALQQVLSTRILAMKASLCKLSPCTVARVCDYHTKLFLMAITSTLKSLLRPHFLNTPDKSPGDRLAEICAKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLVSLPNQGSPLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICCEAVMRAQPVNQMRVWWMNAACCLASCWSPTWTGFPPVMAWSVACSPNSPCACASAERPLCPAAPPPCSWTASPGPLASPRSTTSGGYTWAPTPPRSAKPAPGVAVSPC

Target ORF information:

RefSeq Version	NM_001359629.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 16 (Med16), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359629.1

ORF Insert Sequence:

ATGGCGGCGT CGGATCCCGG GCGTTGGGGC GAGTGTGGAC CGCGGCCGCC GGGCCGACTG 
GGCGCCCGGG GCGGACAGGA GCCAGGGACC GTGCTGGGTG CGGTGGGTAT GATGGACCTG 
GCCTATGTGT GCGAATGGGA GAAGTGGGCC AAGAGCACGT ACTGCCCGTC GCTGCCCCTG 
GCTTGTGCCT GGTCCTGCAG GAACCTCATT GCCTTCACCA CAGACCTACG CAATGATGAC 
CAGGATCTGA CACACATGAT CCATATCCTA GACACGGAAC ACCCCTGGGA AGTGCACTCT 
GTCAGCTCTG GCCACAGCGA GGCCATCACC TGCCTGGAGT GGGACCAGTC AGGGTCCCGG 
CTACTGTCTG CTGATGCTGA TGGACAGATC AAATGCTGGA GCATGGCTGA CCACCTGGCC 
AACAGCTGGG AGAGCTCGGT GGGCAGCCAG GTGGAGGGGG ACCCCATCGT GGCCCTGTCC 
TGGCTGCACA ACGGTGTAAA GCTGGCCCTG CATGTGGAGA AGTCTGGTGC CTCTAGCTTC 
GGAGAGAAGT TCTCACGTGT GAAGTTCTCC CCGTCCCTCA CGCTGTTCGG TGGCAAGCCA 
ATGGAGGGTT GGATTGCAGT GACAGTCAGT GGCCTGGTCA CTGTGTCCCT TCTGAAGCCC 
AGCGGGCAGG TGCTGACGTC CACGGAGAGC CTGTGCCGGC TGCGTGGTAG AGTGGCGCTG 
GCTGACATCG CCTTCACGGG CGGTGGGAAC ATTGTGGTGG CTGCTGCAGA TGGCAGCAGC 
GCGTCACCCG TGAAGTTCTA TAAGGTGTGC GTCAGCGTGG TCAGCGAGAA GTGCCGCATC 
GACACGGAGA TCCTGCCCTC ACTGTTTATG CGCTGCACCA CCGACCCCAA CCGCAAAGAC 
AGGTTCCCCG CCATCACACA CCTCAAGTTC CTGGCGCGAG ACATGTCTGA GCAGGTGCTC 
CTGTGTGCGT CCAGCCAGAC CAGCAGCCTG GTGGAATGCT GGTCCCTTCG GAAAGAGGGC 
CTTCCTGTGA ACAATATCTT CCAGCAGATC TCGCCTGTGG TTGGTGACAA ACAGCCCATG 
ATCCTCAAGT GGCGAATCCT GTCGGCCACC AATGACCTGG ACCGTGTATC CGCGGTGGCA 
CTGCCGAAAC TGCCCATCTC ACTCACCAAC ACTGACCTCA AGGTGGCCAG TGACACCCAG 
TTCTATCCAG GCCTTGGCTT AGCGCTGGCC TTCCAGGATG GCAGCGTGCA CATGGTGCAC 
CGACTGTCCC TGCAGACCAT GGCGGTGTTC TACAGCTCAG CCCCGCGCTC GCTGGATGAG 
CCGGCCCTAA AGCGCCCACG CACCACATGC CCTGCCGTAC ACTTTAAAGC CATGCAGCTG 
TCCTGGACTT CGCTGGCCCT CGTGGGCATC GATAACCATG GGAAGCTCAG CATGCTGCGA 
ATCTCTCCAT CCCTGGGCCA CCCACTGGAG CCCAAGCTGG CCCTGCAGCA CCTGCTTTTC 
CTGCTGGAAT ACTGCATGGT GACCGGCTAT GACTGGTGGG ACATCCTGCT ACACGTGCAG 
CCCGGCATGG TGCAGAGCCT GGTGGAGCGG CTGCATGAGG AGTACACTCG CCAGAAGCCC 
GCCCTGCAAC AGGTCCTCTC CACTCGGATC CTGGCCATGA AGGCTTCACT GTGCAAGCTG 
TCACCCTGCA CAGTGGCTCG TGTGTGTGAC TACCACACCA AGCTGTTCCT CATGGCCATC 
ACGTCCACCC TAAAGTCGCT GCTGCGCCCA CACTTCCTCA ACACCCCTGA CAAGAGCCCT 
GGGGACCGCC TGGCCGAGAT CTGCGCCAAG ATCACCGATG TTGACATCGA CAAAGTCATG 
ATCAACCTGA AGACTGAAGA GTTCGTCCTT GACATGAACA CTTTGCAGGC GCTGCAGCAG 
TTGCTACAGT GGGTGGGAGA CTTCGTGCTC TACCTCCTGG TCAGCCTGCC CAACCAGGGC 
TCCCCGCTAA GGCCGGGCCA CAGCTTCCTC CGAGATGGTA CCTCCCTGGG CATGCTGCGA 
GAGTTGATGG TTGTCATCCG AATCTGGGGC CTGCTGAAGC CCAGCTGTCT GCCCGTCTAC 
ACAGCCACCT CGGACACCCA GGACAGCATG TCCCTGCTCT TCCGACTGCT CACCAAACTG 
TGGATCTGCT GTGAGGCCGT GATGAGGGCG CAGCCAGTGA ACCAGATGAG GGTTTGGTGG 
ATGAATGCTG CCTGCTGCCT AGCCAGCTGC TGGTCCCCAA CTTGGACTGG CTTCCCGCCA 
GTGATGGCCT GGTCAGTCGC CTGCAGCCCA AACAGCCCCT GCGCCTGCGC TTCGGCAGAG 
CGCCCACTCT GCCCAGCAGC ACCTCCACCC TGCAGCTGGA CGGCCTCACC AGGGCCCCTG 
GCCAGCCCAA GATCGACCAC CTCCGGAGGC TACACCTGGG CGCCTACCCC ACCGAGGAGT 
GCAAAGCCTG CACCAGGTGT GGCTGTGTCA CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med16 ( XM_011243402.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241704.1 Mus musculus mediator complex subunit 16 (Med16), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu72948
Clone ID Related Accession (Same CDS sequence)	NM_001359629.1 , XM_011243402.2
Accession Version	XM_011243402.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243402.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGT CGGATCCCGG GCGTTGGGGC GAGTGTGGAC CGCGGCCGCC GGGCCGACTG 
GGCGCCCGGG GCGGACAGGA GCCAGGGACC GTGCTGGGTG CGGTGGGTAT GATGGACCTG 
GCCTATGTGT GCGAATGGGA GAAGTGGGCC AAGAGCACGT ACTGCCCGTC GCTGCCCCTG 
GCTTGTGCCT GGTCCTGCAG GAACCTCATT GCCTTCACCA CAGACCTACG CAATGATGAC 
CAGGATCTGA CACACATGAT CCATATCCTA GACACGGAAC ACCCCTGGGA AGTGCACTCT 
GTCAGCTCTG GCCACAGCGA GGCCATCACC TGCCTGGAGT GGGACCAGTC AGGGTCCCGG 
CTACTGTCTG CTGATGCTGA TGGACAGATC AAATGCTGGA GCATGGCTGA CCACCTGGCC 
AACAGCTGGG AGAGCTCGGT GGGCAGCCAG GTGGAGGGGG ACCCCATCGT GGCCCTGTCC 
TGGCTGCACA ACGGTGTAAA GCTGGCCCTG CATGTGGAGA AGTCTGGTGC CTCTAGCTTC 
GGAGAGAAGT TCTCACGTGT GAAGTTCTCC CCGTCCCTCA CGCTGTTCGG TGGCAAGCCA 
ATGGAGGGTT GGATTGCAGT GACAGTCAGT GGCCTGGTCA CTGTGTCCCT TCTGAAGCCC 
AGCGGGCAGG TGCTGACGTC CACGGAGAGC CTGTGCCGGC TGCGTGGTAG AGTGGCGCTG 
GCTGACATCG CCTTCACGGG CGGTGGGAAC ATTGTGGTGG CTGCTGCAGA TGGCAGCAGC 
GCGTCACCCG TGAAGTTCTA TAAGGTGTGC GTCAGCGTGG TCAGCGAGAA GTGCCGCATC 
GACACGGAGA TCCTGCCCTC ACTGTTTATG CGCTGCACCA CCGACCCCAA CCGCAAAGAC 
AGGTTCCCCG CCATCACACA CCTCAAGTTC CTGGCGCGAG ACATGTCTGA GCAGGTGCTC 
CTGTGTGCGT CCAGCCAGAC CAGCAGCCTG GTGGAATGCT GGTCCCTTCG GAAAGAGGGC 
CTTCCTGTGA ACAATATCTT CCAGCAGATC TCGCCTGTGG TTGGTGACAA ACAGCCCATG 
ATCCTCAAGT GGCGAATCCT GTCGGCCACC AATGACCTGG ACCGTGTATC CGCGGTGGCA 
CTGCCGAAAC TGCCCATCTC ACTCACCAAC ACTGACCTCA AGGTGGCCAG TGACACCCAG 
TTCTATCCAG GCCTTGGCTT AGCGCTGGCC TTCCAGGATG GCAGCGTGCA CATGGTGCAC 
CGACTGTCCC TGCAGACCAT GGCGGTGTTC TACAGCTCAG CCCCGCGCTC GCTGGATGAG 
CCGGCCCTAA AGCGCCCACG CACCACATGC CCTGCCGTAC ACTTTAAAGC CATGCAGCTG 
TCCTGGACTT CGCTGGCCCT CGTGGGCATC GATAACCATG GGAAGCTCAG CATGCTGCGA 
ATCTCTCCAT CCCTGGGCCA CCCACTGGAG CCCAAGCTGG CCCTGCAGCA CCTGCTTTTC 
CTGCTGGAAT ACTGCATGGT GACCGGCTAT GACTGGTGGG ACATCCTGCT ACACGTGCAG 
CCCGGCATGG TGCAGAGCCT GGTGGAGCGG CTGCATGAGG AGTACACTCG CCAGAAGCCC 
GCCCTGCAAC AGGTCCTCTC CACTCGGATC CTGGCCATGA AGGCTTCACT GTGCAAGCTG 
TCACCCTGCA CAGTGGCTCG TGTGTGTGAC TACCACACCA AGCTGTTCCT CATGGCCATC 
ACGTCCACCC TAAAGTCGCT GCTGCGCCCA CACTTCCTCA ACACCCCTGA CAAGAGCCCT 
GGGGACCGCC TGGCCGAGAT CTGCGCCAAG ATCACCGATG TTGACATCGA CAAAGTCATG 
ATCAACCTGA AGACTGAAGA GTTCGTCCTT GACATGAACA CTTTGCAGGC GCTGCAGCAG 
TTGCTACAGT GGGTGGGAGA CTTCGTGCTC TACCTCCTGG TCAGCCTGCC CAACCAGGGC 
TCCCCGCTAA GGCCGGGCCA CAGCTTCCTC CGAGATGGTA CCTCCCTGGG CATGCTGCGA 
GAGTTGATGG TTGTCATCCG AATCTGGGGC CTGCTGAAGC CCAGCTGTCT GCCCGTCTAC 
ACAGCCACCT CGGACACCCA GGACAGCATG TCCCTGCTCT TCCGACTGCT CACCAAACTG 
TGGATCTGCT GTGAGGCCGT GATGAGGGCG CAGCCAGTGA ACCAGATGAG GGTTTGGTGG 
ATGAATGCTG CCTGCTGCCT AGCCAGCTGC TGGTCCCCAA CTTGGACTGG CTTCCCGCCA 
GTGATGGCCT GGTCAGTCGC CTGCAGCCCA AACAGCCCCT GCGCCTGCGC TTCGGCAGAG 
CGCCCACTCT GCCCAGCAGC ACCTCCACCC TGCAGCTGGA CGGCCTCACC AGGGCCCCTG 
GCCAGCCCAA GATCGACCAC CTCCGGAGGC TACACCTGGG CGCCTACCCC ACCGAGGAGT 
GCAAAGCCTG CACCAGGTGT GGCTGTGTCA CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241704.1
CDS	47..2545
Translation	MAASDPGRWGECGPRPPGRLGARGGQEPGTVLGAVGMMDLAYVCEWEKWAKSTYCPSLPLACAWSCRNLIAFTTDLRNDDQDLTHMIHILDTEHPWEVHSVSSGHSEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWESSVGSQVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVAAADGSSASPVKFYKVCVSVVSEKCRIDTEILPSLFMRCTTDPNRKDRFPAITHLKFLARDMSEQVLLCASSQTSSLVECWSLRKEGLPVNNIFQQISPVVGDKQPMILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFQDGSVHMVHRLSLQTMAVFYSSAPRSLDEPALKRPRTTCPAVHFKAMQLSWTSLALVGIDNHGKLSMLRISPSLGHPLEPKLALQHLLFLLEYCMVTGYDWWDILLHVQPGMVQSLVERLHEEYTRQKPALQQVLSTRILAMKASLCKLSPCTVARVCDYHTKLFLMAITSTLKSLLRPHFLNTPDKSPGDRLAEICAKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLVSLPNQGSPLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICCEAVMRAQPVNQMRVWWMNAACCLASCWSPTWTGFPPVMAWSVACSPNSPCACASAERPLCPAAPPPCSWTASPGPLASPRSTTSGGYTWAPTPPRSAKPAPGVAVSPC

Target ORF information:

RefSeq Version	XM_011243402.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 16 (Med16), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243402.2

ORF Insert Sequence:

ATGGCGGCGT CGGATCCCGG GCGTTGGGGC GAGTGTGGAC CGCGGCCGCC GGGCCGACTG 
GGCGCCCGGG GCGGACAGGA GCCAGGGACC GTGCTGGGTG CGGTGGGTAT GATGGACCTG 
GCCTATGTGT GCGAATGGGA GAAGTGGGCC AAGAGCACGT ACTGCCCGTC GCTGCCCCTG 
GCTTGTGCCT GGTCCTGCAG GAACCTCATT GCCTTCACCA CAGACCTACG CAATGATGAC 
CAGGATCTGA CACACATGAT CCATATCCTA GACACGGAAC ACCCCTGGGA AGTGCACTCT 
GTCAGCTCTG GCCACAGCGA GGCCATCACC TGCCTGGAGT GGGACCAGTC AGGGTCCCGG 
CTACTGTCTG CTGATGCTGA TGGACAGATC AAATGCTGGA GCATGGCTGA CCACCTGGCC 
AACAGCTGGG AGAGCTCGGT GGGCAGCCAG GTGGAGGGGG ACCCCATCGT GGCCCTGTCC 
TGGCTGCACA ACGGTGTAAA GCTGGCCCTG CATGTGGAGA AGTCTGGTGC CTCTAGCTTC 
GGAGAGAAGT TCTCACGTGT GAAGTTCTCC CCGTCCCTCA CGCTGTTCGG TGGCAAGCCA 
ATGGAGGGTT GGATTGCAGT GACAGTCAGT GGCCTGGTCA CTGTGTCCCT TCTGAAGCCC 
AGCGGGCAGG TGCTGACGTC CACGGAGAGC CTGTGCCGGC TGCGTGGTAG AGTGGCGCTG 
GCTGACATCG CCTTCACGGG CGGTGGGAAC ATTGTGGTGG CTGCTGCAGA TGGCAGCAGC 
GCGTCACCCG TGAAGTTCTA TAAGGTGTGC GTCAGCGTGG TCAGCGAGAA GTGCCGCATC 
GACACGGAGA TCCTGCCCTC ACTGTTTATG CGCTGCACCA CCGACCCCAA CCGCAAAGAC 
AGGTTCCCCG CCATCACACA CCTCAAGTTC CTGGCGCGAG ACATGTCTGA GCAGGTGCTC 
CTGTGTGCGT CCAGCCAGAC CAGCAGCCTG GTGGAATGCT GGTCCCTTCG GAAAGAGGGC 
CTTCCTGTGA ACAATATCTT CCAGCAGATC TCGCCTGTGG TTGGTGACAA ACAGCCCATG 
ATCCTCAAGT GGCGAATCCT GTCGGCCACC AATGACCTGG ACCGTGTATC CGCGGTGGCA 
CTGCCGAAAC TGCCCATCTC ACTCACCAAC ACTGACCTCA AGGTGGCCAG TGACACCCAG 
TTCTATCCAG GCCTTGGCTT AGCGCTGGCC TTCCAGGATG GCAGCGTGCA CATGGTGCAC 
CGACTGTCCC TGCAGACCAT GGCGGTGTTC TACAGCTCAG CCCCGCGCTC GCTGGATGAG 
CCGGCCCTAA AGCGCCCACG CACCACATGC CCTGCCGTAC ACTTTAAAGC CATGCAGCTG 
TCCTGGACTT CGCTGGCCCT CGTGGGCATC GATAACCATG GGAAGCTCAG CATGCTGCGA 
ATCTCTCCAT CCCTGGGCCA CCCACTGGAG CCCAAGCTGG CCCTGCAGCA CCTGCTTTTC 
CTGCTGGAAT ACTGCATGGT GACCGGCTAT GACTGGTGGG ACATCCTGCT ACACGTGCAG 
CCCGGCATGG TGCAGAGCCT GGTGGAGCGG CTGCATGAGG AGTACACTCG CCAGAAGCCC 
GCCCTGCAAC AGGTCCTCTC CACTCGGATC CTGGCCATGA AGGCTTCACT GTGCAAGCTG 
TCACCCTGCA CAGTGGCTCG TGTGTGTGAC TACCACACCA AGCTGTTCCT CATGGCCATC 
ACGTCCACCC TAAAGTCGCT GCTGCGCCCA CACTTCCTCA ACACCCCTGA CAAGAGCCCT 
GGGGACCGCC TGGCCGAGAT CTGCGCCAAG ATCACCGATG TTGACATCGA CAAAGTCATG 
ATCAACCTGA AGACTGAAGA GTTCGTCCTT GACATGAACA CTTTGCAGGC GCTGCAGCAG 
TTGCTACAGT GGGTGGGAGA CTTCGTGCTC TACCTCCTGG TCAGCCTGCC CAACCAGGGC 
TCCCCGCTAA GGCCGGGCCA CAGCTTCCTC CGAGATGGTA CCTCCCTGGG CATGCTGCGA 
GAGTTGATGG TTGTCATCCG AATCTGGGGC CTGCTGAAGC CCAGCTGTCT GCCCGTCTAC 
ACAGCCACCT CGGACACCCA GGACAGCATG TCCCTGCTCT TCCGACTGCT CACCAAACTG 
TGGATCTGCT GTGAGGCCGT GATGAGGGCG CAGCCAGTGA ACCAGATGAG GGTTTGGTGG 
ATGAATGCTG CCTGCTGCCT AGCCAGCTGC TGGTCCCCAA CTTGGACTGG CTTCCCGCCA 
GTGATGGCCT GGTCAGTCGC CTGCAGCCCA AACAGCCCCT GCGCCTGCGC TTCGGCAGAG 
CGCCCACTCT GCCCAGCAGC ACCTCCACCC TGCAGCTGGA CGGCCTCACC AGGGCCCCTG 
GCCAGCCCAA GATCGACCAC CTCCGGAGGC TACACCTGGG CGCCTACCCC ACCGAGGAGT 
GCAAAGCCTG CACCAGGTGT GGCTGTGTCA CCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Mediator Subunit MED16 Transduces NRF2-Activating Signals into Antioxidant Gene Expression.
Molecular and cellular biology36(3)407-20(2015 Nov)
Sekine H,Okazaki K,Ota N,Shima H,Katoh Y,Suzuki N,Igarashi K,Ito M,Motohashi H,Yamamoto M

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
EMBO reports11(7)541-7(2010 Jul)
Krebs AR,Demmers J,Karmodiya K,Chang NC,Chang AC,Tora L

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Diversification of stem cell molecular repertoire by alternative splicing.
Proceedings of the National Academy of Sciences of the United States of America102(40)14290-5(2005 Oct)
Pritsker M,Doniger TT,Kramer LC,Westcot SE,Lemischka IR

Structural and functional organization of TRAP220, the TRAP/mediator subunit that is targeted by nuclear receptors.
Molecular and cellular biology24(18)8244-54(2004 Sep)
Malik S,Guermah M,Yuan CX,Wu W,Yamamura S,Roeder RG

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Med16 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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