gRNAs
The following MED16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu04977 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_005481.2 , NM_005481.3 | |
| Accession Version | NM_005481.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-30 | |
| Organism | Homo sapiens(human) | |
| Product | mediator of RNA polymerase II transcription subunit 16 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU098434.1, BI461485.1, BC017282.2, CB050542.1, BQ015734.1 and AL137732.1. On or before Mar 4, 2006 this sequence version replaced XM_939640.1, NM_005481.1. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.225385.1, SRR1803617.154811.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGTGTGATT TGCGGCGGCC AGCGGCAGGT GGGATGATGG ACTTGGCCTA CGTCTGTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_005472.2 |
| CDS | 152..2785 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_005481.2 |
1 | ATGTGTGATT TGCGGCGGCC AGCGGCAGGT GGGATGATGG ACTTGGCCTA CGTCTGTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu04977 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_005481.2 , NM_005481.3 | |
| Accession Version | NM_005481.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-09 | |
| Organism | Homo sapiens(human) | |
| Product | mediator of RNA polymerase II transcription subunit 16 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI461485.1, BC017282.2, CB050542.1, BQ015734.1 and AL137732.1. On Nov 23, 2018 this sequence version replaced NM_005481.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.881826.1, SRR1803613.225385.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000325464.6/ ENSP00000325612.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGTGTGATT TGCGGCGGCC AGCGGCAGGT GGGATGATGG ACTTGGCCTA CGTCTGTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_005472.2 |
| CDS | 121..2754 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_005481.3 |
1 | ATGTGTGATT TGCGGCGGCC AGCGGCAGGT GGGATGATGG ACTTGGCCTA CGTCTGTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu88103 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017026121.1 | |
| Accession Version | XM_017026121.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | mediator of RNA polymerase II transcription subunit 16 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGGGCT GGATCGCGGT GACGGTCAGC GGCCTGGTCA CCGTGTCCCT GCTGAAGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016881610.1 |
| CDS | 517..2625 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017026121.1 |
1 | ATGGAGGGCT GGATCGCGGT GACGGTCAGC GGCCTGGTCA CCGTGTCCCT GCTGAAGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu88102 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017026120.2 , XM_017026120.1 | |
| Accession Version | XM_017026120.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | mediator of RNA polymerase II transcription subunit 16 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017026120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTGTGATT TGCGGCGGCC AGCGGCAGGT GGGATGATGG ACTTGGCCTA CGTCTGTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016881609.1 |
| CDS | 142..2568 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017026120.2 |
1 | ATGTGTGATT TGCGGCGGCC AGCGGCAGGT GGGATGATGG ACTTGGCCTA CGTCTGTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu88102 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017026120.2 , XM_017026120.1 | |
| Accession Version | XM_017026120.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-05 | |
| Organism | Homo sapiens(human) | |
| Product | mediator of RNA polymerase II transcription subunit 16 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTGTGATT TGCGGCGGCC AGCGGCAGGT GGGATGATGG ACTTGGCCTA CGTCTGTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016881609.1 |
| CDS | 147..2573 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017026120.1 |
1 | ATGTGTGATT TGCGGCGGCC AGCGGCAGGT GGGATGATGG ACTTGGCCTA CGTCTGTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Homozygous MED25 mutation implicated in eye-intellectual disability syndrome. |
Subunit architecture and functional modular rearrangements of the transcriptional mediator complex. |
Defining the membrane proteome of NK cells. |
A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. |
A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. |
