Mylk3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mylk3
Entrez Gene ID	213435
Full Name	myosin light chain kinase 3
Synonyms	D830007F02Rik,MLCK
General protein information	Preferred Names myosin light chain kinase 3 Names myosin light chain kinase 3 cardiac MLCK cardiac-MyBP-C-associated Ca/CaM kinase cardiac-specific myosin light chain kinase putative myosin light chain kinase 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_175441.5	NP_780650.2	myosin light chain kinase 3 isoform 1
XM_006530825.2	XP_006530888.1	myosin light chain kinase 3 isoform X1
NM_001297612.1	NP_001284541.1	myosin light chain kinase 3 isoform 2
XM_006530825.3	XP_006530888.1	myosin light chain kinase 3 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYLK3	XP_003315132.1
	MYLK3	XP_001113601.1
Canis lupus familiaris (dog)	MYLK3	XP_532569.4
Bos taurus (cattle)	MYLK3	XP_005218847.1
Mus musculus (house mouse)	Mylk3	NP_780650.2
Homo sapiens (human)	MYLK3	NP_872299.2
Rattus norvegicus (Norway rat)	Mylk3	NP_001104280.1
Gallus gallus (chicken)	MYLK3	XP_004944251.1
Xenopus tropicalis (tropical clawed frog)	mylk3	XP_002931740.2

Gene Ontology
Bibliography

Related articles in PubMed

Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation.
Song KH, Lee J, Jung HR, Park H, Doh J
Scientific reports7(1)11533(2017 Sep)

Han J, Li JH, Bai G, Shen GS, Chen J, Liu JN, Wang S, Liu XJ
World journal of gastroenterology23(12)2175-2184(2017 Mar)

Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments.
Beach JR, Bruun KS, Shao L, Li D, Swider Z, Remmert K, Zhang Y, Conti MA, Adelstein RS, Rusan NM, Betzig E, Hammer JA
Nature cell biology19(2)85-93(2017 Feb)

Cardiac myosin light chain is phosphorylated by Ca2+/calmodulin-dependent and -independent kinase activities.
Chang AN, Mahajan P, Knapp S, Barton H, Sweeney HL, Kamm KE, Stull JT
Proceedings of the National Academy of Sciences of the United States of America113(27)E3824-33(2016 07)

The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.
G?mez-Del Arco P, Perdiguero E, Yunes-Leites PS, Ac?n-P?rez R, Zeini M, Garcia-Gomez A, Sreenivasan K, Jim?nez-Alc?zar M, Segal?s J, L?pez-Maderuelo D, Orn?s B, Jim?nez-Borreguero LJ, D'Amato G, Enshell-Seijffers D, Morgan B, Georgopoulos K, Islam AB, Braun T, de la Pompa JL, Kim J, Enriquez JA, Ballestar E, Mu?oz-C?noves P, Redondo JM
Cell metabolism23(5)881-92(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Acanthopanax senticosus polysaccharide pretreatment may be associated with inhibition of the NF-kappaB/MLCK pathway and concomitant amelioration of LPS-induced tight junction dysfunction of intestinal epithelium in endotoxemia

that MLCK is competing with centrally active ROCK for a limiting pool of monomer with which to drive lamellar filament assembly
Title: Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments.

This study demonstrated that catchlike property during dynamic performance in mouse hindlimb muscles with (wild-type) and without MLCK(-/-) ) the primary mechanism for PTP (myosin phosphorylation).
Title: Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle.

Ablation of cardiac Mylk3 suggests that Mylk3 plays a pivotal role in the transition from compensated to decompensated hypertrophy via cardiac myocyte atrophy and sarcomeric disorganization.
Title: Acute heart failure with cardiomyocyte atrophy induced in adult mice by ablation of cardiac myosin light chain kinase.

Data suggest that galectin-3 in hepatocellular carcinoma cells promotes cell migration by inducing RhoA GTPase activity, activating Mlck, up-regulating phosphorylation of MLC2 (myosin light chain 2), and inducing actin rearrangement.
Title: Galectin 3 regulates HCC cell invasion by RhoA and MLCK activation.

The following Mylk3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mylk3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23768	NM_001297612.1 Latest version!	Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu23765	NM_175441.5 Latest version!	Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu41328	XM_006530825.3 Latest version!	Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu41328	XM_006530825.2	Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mylk3 ( NM_001297612.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001284541.1 Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23768
Clone ID Related Accession (Same CDS sequence)	NM_001297612.1
Accession Version	NM_001297612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB843262.1, AK165216.1 and AK146683.1. On Jul 4, 2014 this sequence version replaced XM_006530824.1. Transcript Variant: This variant (2) uses an alternate first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK165216.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140 [ECO:0000348] ##Evidence-Data-END##

ATGGGAACTC TGTGCCCTGT CCCAGCACTG TGCAGGCCCA GCACCAGAGG CCAGCAGCGG 
GCATCAGATT ACACCCAGAT ATTTGCGAAC AAGGAGCAAG CAGAAGTTGC TGGAGTGAAG 
CCAAACCATG TACTGACTAC AGGAGGTGTG CAAGCTGACG CCTCTAGGAC GCTGTGGGAA 
GAGAGCCAAA AGGAGGACAT ACCTGTGCGA ACAGTGGAGG GGCTGCCTCT CATCATCAAT 
ACGTCACTGA AGGGAGCTGA CCTAACCCAG GCAGGAGCCT CACTGAGGCA GGGAGTTGAA 
GTTCTTGGCC CAGGCCAAGT ACCCCTACCC ACAGAAGCAG AATCCAGGCT TCCTGAGACA 
GCCAGTGAGA ACACTGGAGC CACCCTGGAA TTGTCCGTAG CAATCGACAG AATCAGCGAG 
GTCCTCACTA GCCTCAAGAT GTCACAAGGT GGTGGTCAAG AAACCTCATC CAGCAAGCCT 
GACTGTTGGC TTTCAGAAGA GGCCATGAGG CTGAGTTCAG GGCCTCTCCC TCAGCCCCTA 
GGACCACTAA CTCCAGACAG TGACATTCAC AGTGGTGATG CACTTCCCAG GATCCCTATC 
AATATGCAAG AGATGGCTAC TCCTGGGGAG TTGCTTGAGA CCCAAAGTGG CAGTCCCATT 
GGCTCTGCAG AAGCTCCAGG CCTTGGGACT GTGTTAGAAG ACCAGATCCC TAAAGGAGCC 
AGACCATTTC CACCCCTGCC AAAGAGGAGC AGCAACAATG GTGGCATGAG TGCAGAGGAG 
GAGATAGGGT CTGGGGCTGA GCCTATGAGA GGACCAAGCT TGGCTACAAG GGACTGGAGA 
GATGAGACTG TTGGGACCAC AGACCTGCAG CAAGGCATAG ACCCAGGAGC AGTGAGCCCT 
GAGCCTGGGA AGGACCACGC AGCCCAGGGC CCAGGAAGAA CTGAAGCTGG AAGGCTATCT 
TCTGCTGCAG AGGCTGCCAT TGTGGTTCTA GATGACAGCG CAGCACCCCC AGCCCCTTTT 
GAACACCGGG TAGTGAGCAT CAAAGATACC CTGATCTCAG CAGGCTACAC GGTATCCCAA 
CATGAAGTCT TAGGAGGGGG TCGGTTTGGC CAGGTGCACA GGTGTACAGA GAGGTCTACA 
GGCCTTGCAC TGGCAGCCAA GATCATCAAA GTGAAGAACG TAAAGGACCG GGAGGATGTG 
AAGAATGAGG TCAACATCAT GAACCAGCTC AGCCACGTAA ACTTGATCCA ACTTTATGAT 
GCGTTTGAGA GCAAGAGCAG CTTCACTCTG ATCATGGAGT ATGTGGATGG AGGCGAACTC 
TTTGACCGGA TCACGGATGA GAAGTACCAC CTCACTGAGT TGGATGTGGT CTTGTTCACG 
AGGCAGATCT GTGAGGGTGT GCATTACCTG CATCAGCACT ATATCCTGCA CCTGGACCTC 
AAGCCTGAGA ACATATTGTG TGTCAGCCAG ACAGGGCATC AAATTAAGAT CATTGACTTT 
GGGCTGGCTA GAAGATACAA GCCTCGGGAG AAGCTAAAGG TGAACTTTGG TACTCCGGAG 
TTCCTGGCCC CAGAAGTTGT TAACTATGAG TTTGTGTCAT TTCCAACAGA CATGTGGAGT 
GTGGGAGTTA TCACCTACAT GCTACTCAGT GGTTTGTCCC CATTTCTAGG GGAGACAGAT 
GCAGAGACCA TGAATTTTAT TGTGAACTGC AGCTGGGATT TCGATGCTGA TACCTTCAAA 
GGGCTGTCGG AGGAAGCCAA GGACTTTGTT TCCCGGTTAC TGGTCAAAGA GAAGAGCTGT 
AGGATGAGCG CCACACAGTG CCTGAAACAC GAGTGGTTAA GTCACCTGCC TGCCAAAGCC 
TCGGGCTCCA ACGTTCGCCT CAGATCCCAA CAACTGCTGC AGAAATATAT GGCTCAGAGT 
AAATGGAAGA AACATTTCCA CGTGGTGACT GCAGTCAACA GGCTACGGAA ATTTCCAACG 
TGTCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284541.1
CDS	97..2085
Translation	MGTLCPVPALCRPSTRGQQRASDYTQIFANKEQAEVAGVKPNHVLTTGGVQADASRTLWEESQKEDIPVRTVEGLPLIINTSLKGADLTQAGASLRQGVEVLGPGQVPLPTEAESRLPETASENTGATLELSVAIDRISEVLTSLKMSQGGGQETSSSKPDCWLSEEAMRLSSGPLPQPLGPLTPDSDIHSGDALPRIPINMQEMATPGELLETQSGSPIGSAEAPGLGTVLEDQIPKGARPFPPLPKRSSNNGGMSAEEEIGSGAEPMRGPSLATRDWRDETVGTTDLQQGIDPGAVSPEPGKDHAAQGPGRTEAGRLSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDTLISAGYTVSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNVKDREDVKNEVNIMNQLSHVNLIQLYDAFESKSSFTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQTGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLSHLPAKASGSNVRLRSQQLLQKYMAQSKWKKHFHVVTAVNRLRKFPTCP

Target ORF information:

RefSeq Version	NM_001297612.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297612.1

ORF Insert Sequence:

ATGGGAACTC TGTGCCCTGT CCCAGCACTG TGCAGGCCCA GCACCAGAGG CCAGCAGCGG 
GCATCAGATT ACACCCAGAT ATTTGCGAAC AAGGAGCAAG CAGAAGTTGC TGGAGTGAAG 
CCAAACCATG TACTGACTAC AGGAGGTGTG CAAGCTGACG CCTCTAGGAC GCTGTGGGAA 
GAGAGCCAAA AGGAGGACAT ACCTGTGCGA ACAGTGGAGG GGCTGCCTCT CATCATCAAT 
ACGTCACTGA AGGGAGCTGA CCTAACCCAG GCAGGAGCCT CACTGAGGCA GGGAGTTGAA 
GTTCTTGGCC CAGGCCAAGT ACCCCTACCC ACAGAAGCAG AATCCAGGCT TCCTGAGACA 
GCCAGTGAGA ACACTGGAGC CACCCTGGAA TTGTCCGTAG CAATCGACAG AATCAGCGAG 
GTCCTCACTA GCCTCAAGAT GTCACAAGGT GGTGGTCAAG AAACCTCATC CAGCAAGCCT 
GACTGTTGGC TTTCAGAAGA GGCCATGAGG CTGAGTTCAG GGCCTCTCCC TCAGCCCCTA 
GGACCACTAA CTCCAGACAG TGACATTCAC AGTGGTGATG CACTTCCCAG GATCCCTATC 
AATATGCAAG AGATGGCTAC TCCTGGGGAG TTGCTTGAGA CCCAAAGTGG CAGTCCCATT 
GGCTCTGCAG AAGCTCCAGG CCTTGGGACT GTGTTAGAAG ACCAGATCCC TAAAGGAGCC 
AGACCATTTC CACCCCTGCC AAAGAGGAGC AGCAACAATG GTGGCATGAG TGCAGAGGAG 
GAGATAGGGT CTGGGGCTGA GCCTATGAGA GGACCAAGCT TGGCTACAAG GGACTGGAGA 
GATGAGACTG TTGGGACCAC AGACCTGCAG CAAGGCATAG ACCCAGGAGC AGTGAGCCCT 
GAGCCTGGGA AGGACCACGC AGCCCAGGGC CCAGGAAGAA CTGAAGCTGG AAGGCTATCT 
TCTGCTGCAG AGGCTGCCAT TGTGGTTCTA GATGACAGCG CAGCACCCCC AGCCCCTTTT 
GAACACCGGG TAGTGAGCAT CAAAGATACC CTGATCTCAG CAGGCTACAC GGTATCCCAA 
CATGAAGTCT TAGGAGGGGG TCGGTTTGGC CAGGTGCACA GGTGTACAGA GAGGTCTACA 
GGCCTTGCAC TGGCAGCCAA GATCATCAAA GTGAAGAACG TAAAGGACCG GGAGGATGTG 
AAGAATGAGG TCAACATCAT GAACCAGCTC AGCCACGTAA ACTTGATCCA ACTTTATGAT 
GCGTTTGAGA GCAAGAGCAG CTTCACTCTG ATCATGGAGT ATGTGGATGG AGGCGAACTC 
TTTGACCGGA TCACGGATGA GAAGTACCAC CTCACTGAGT TGGATGTGGT CTTGTTCACG 
AGGCAGATCT GTGAGGGTGT GCATTACCTG CATCAGCACT ATATCCTGCA CCTGGACCTC 
AAGCCTGAGA ACATATTGTG TGTCAGCCAG ACAGGGCATC AAATTAAGAT CATTGACTTT 
GGGCTGGCTA GAAGATACAA GCCTCGGGAG AAGCTAAAGG TGAACTTTGG TACTCCGGAG 
TTCCTGGCCC CAGAAGTTGT TAACTATGAG TTTGTGTCAT TTCCAACAGA CATGTGGAGT 
GTGGGAGTTA TCACCTACAT GCTACTCAGT GGTTTGTCCC CATTTCTAGG GGAGACAGAT 
GCAGAGACCA TGAATTTTAT TGTGAACTGC AGCTGGGATT TCGATGCTGA TACCTTCAAA 
GGGCTGTCGG AGGAAGCCAA GGACTTTGTT TCCCGGTTAC TGGTCAAAGA GAAGAGCTGT 
AGGATGAGCG CCACACAGTG CCTGAAACAC GAGTGGTTAA GTCACCTGCC TGCCAAAGCC 
TCGGGCTCCA ACGTTCGCCT CAGATCCCAA CAACTGCTGC AGAAATATAT GGCTCAGAGT 
AAATGGAAGA AACATTTCCA CGTGGTGACT GCAGTCAACA GGCTACGGAA ATTTCCAACG 
TGTCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk3 ( XM_006530825.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530888.1 Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu41328
Clone ID Related Accession (Same CDS sequence)	XM_006530825.2 , XM_006530825.3
Accession Version	XM_006530825.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACA AGGAGCAAGC AGAAGTTGCT GGAGTGAAGC CAAACCATGT ACTGACTACA 
GGAGGTGTGC AAGCTGACGC CTCTAGGACG CTGTGGGAAG AGAGCCAAAA GGAGGACATA 
CCTGTGCGAA CAGTGGAGGG GCTGCCTCTC ATCATCAATA CGTCACTGAA GGGAGCTGAC 
CTAACCCAGG CAGGAGCCTC ACTGAGGCAG GGAGTTGAAG TTCTTGGCCC AGGCCAAGTA 
CCCCTACCCA CAGAAGCAGA ATCCAGGCTT CCTGAGACAG CCAGTGAGAA CACTGGAGCC 
ACCCTGGAAT TGTCCGTAGC AATCGACAGA ATCAGCGAGG TCCTCACTAG CCTCAAGATG 
TCACAAGGTG GTGGTCAAGA AACCTCATCC AGCAAGCCTG ACTGTTGGCT TTCAGAAGAG 
GCCATGAGGC TGAGTTCAGG GCCTCTCCCT CAGCCCCTAG GACCACTAAC TCCAGACAGT 
GACATTCACA GTGGTGATGC ACTTCCCAGG ATCCCTATCA ATATGCAAGA GATGGCTACT 
CCTGGGGAGT TGCTTGAGAC CCAAAGTGGC AGTCCCATTG GCTCTGCAGA AGCTCCAGGC 
CTTGGGACTG TGTTAGAAGA CCAGATCCCT AAAGGAGCCA GACCATTTCC ACCCCTGCCA 
AAGAGGAGCA GCAACAATGG TGGCATGAGT GCAGAGGAGG AGATAGGGTC TGGGGCTGAG 
CCTATGAGAG GACCAAGCTT GGCTACAAGG GACTGGAGAG ATGAGACTGT TGGGACCACA 
GACCTGCAGC AAGGCATAGA CCCAGGAGCA GTGAGCCCTG AGCCTGGGAA GGACCACGCA 
GCCCAGGGCC CAGGAAGAAC TGAAGCTGGA AGGCTATCTT CTGCTGCAGA GGCTGCCATT 
GTGGTTCTAG ATGACAGCGC AGCACCCCCA GCCCCTTTTG AACACCGGGT AGTGAGCATC 
AAAGATACCC TGATCTCAGC AGGCTACACG GTATCCCAAC ATGAAGTCTT AGGAGGGGGT 
CGGTTTGGCC AGGTGCACAG GTGTACAGAG AGGTCTACAG GCCTTGCACT GGCAGCCAAG 
ATCATCAAAG TGAAGAACGT AAAGGACCGG GAGGATGTGA AGAATGAGGT CAACATCATG 
AACCAGCTCA GCCACGTAAA CTTGATCCAA CTTTATGATG CGTTTGAGAG CAAGAGCAGC 
TTCACTCTGA TCATGGAGTA TGTGGATGGA GGCGAACTCT TTGACCGGAT CACGGATGAG 
AAGTACCACC TCACTGAGTT GGATGTGGTC TTGTTCACGA GGCAGATCTG TGAGGGTGTG 
CATTACCTGC ATCAGCACTA TATCCTGCAC CTGGACCTCA AGCCTGAGAA CATATTGTGT 
GTCAGCCAGA CAGGGCATCA AATTAAGATC ATTGACTTTG GGCTGGCTAG AAGATACAAG 
CCTCGGGAGA AGCTAAAGGT GAACTTTGGT ACTCCGGAGT TCCTGGCCCC AGAAGTTGTT 
AACTATGAGT TTGTGTCATT TCCAACAGAC ATGTGGAGTG TGGGAGTTAT CACCTACATG 
CTACTCAGTG GTTTGTCCCC ATTTCTAGGG GAGACAGATG CAGAGACCAT GAATTTTATT 
GTGAACTGCA GCTGGGATTT CGATGCTGAT ACCTTCAAAG GGCTGTCGGA GGAAGCCAAG 
GACTTTGTTT CCCGGTTACT GGTCAAAGAG AAGAGCTGTA GGATGAGCGC CACACAGTGC 
CTGAAACACG AGTGGTTAAG TCACCTGCCT GCCAAAGCCT CGGGCTCCAA CGTTCGCCTC 
AGATCCCAAC AACTGCTGCA GAAATATATG GCTCAGAGTA AATGGAAGAA ACATTTCCAC 
GTGGTGACTG CAGTCAACAG GCTACGGAAA TTTCCAACGT GTCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530888.1
CDS	228..2135
Translation	MKNKEQAEVAGVKPNHVLTTGGVQADASRTLWEESQKEDIPVRTVEGLPLIINTSLKGADLTQAGASLRQGVEVLGPGQVPLPTEAESRLPETASENTGATLELSVAIDRISEVLTSLKMSQGGGQETSSSKPDCWLSEEAMRLSSGPLPQPLGPLTPDSDIHSGDALPRIPINMQEMATPGELLETQSGSPIGSAEAPGLGTVLEDQIPKGARPFPPLPKRSSNNGGMSAEEEIGSGAEPMRGPSLATRDWRDETVGTTDLQQGIDPGAVSPEPGKDHAAQGPGRTEAGRLSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDTLISAGYTVSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNVKDREDVKNEVNIMNQLSHVNLIQLYDAFESKSSFTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQTGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLSHLPAKASGSNVRLRSQQLLQKYMAQSKWKKHFHVVTAVNRLRKFPTCP

Target ORF information:

RefSeq Version	XM_006530825.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530825.2

ORF Insert Sequence:

ATGAAGAACA AGGAGCAAGC AGAAGTTGCT GGAGTGAAGC CAAACCATGT ACTGACTACA 
GGAGGTGTGC AAGCTGACGC CTCTAGGACG CTGTGGGAAG AGAGCCAAAA GGAGGACATA 
CCTGTGCGAA CAGTGGAGGG GCTGCCTCTC ATCATCAATA CGTCACTGAA GGGAGCTGAC 
CTAACCCAGG CAGGAGCCTC ACTGAGGCAG GGAGTTGAAG TTCTTGGCCC AGGCCAAGTA 
CCCCTACCCA CAGAAGCAGA ATCCAGGCTT CCTGAGACAG CCAGTGAGAA CACTGGAGCC 
ACCCTGGAAT TGTCCGTAGC AATCGACAGA ATCAGCGAGG TCCTCACTAG CCTCAAGATG 
TCACAAGGTG GTGGTCAAGA AACCTCATCC AGCAAGCCTG ACTGTTGGCT TTCAGAAGAG 
GCCATGAGGC TGAGTTCAGG GCCTCTCCCT CAGCCCCTAG GACCACTAAC TCCAGACAGT 
GACATTCACA GTGGTGATGC ACTTCCCAGG ATCCCTATCA ATATGCAAGA GATGGCTACT 
CCTGGGGAGT TGCTTGAGAC CCAAAGTGGC AGTCCCATTG GCTCTGCAGA AGCTCCAGGC 
CTTGGGACTG TGTTAGAAGA CCAGATCCCT AAAGGAGCCA GACCATTTCC ACCCCTGCCA 
AAGAGGAGCA GCAACAATGG TGGCATGAGT GCAGAGGAGG AGATAGGGTC TGGGGCTGAG 
CCTATGAGAG GACCAAGCTT GGCTACAAGG GACTGGAGAG ATGAGACTGT TGGGACCACA 
GACCTGCAGC AAGGCATAGA CCCAGGAGCA GTGAGCCCTG AGCCTGGGAA GGACCACGCA 
GCCCAGGGCC CAGGAAGAAC TGAAGCTGGA AGGCTATCTT CTGCTGCAGA GGCTGCCATT 
GTGGTTCTAG ATGACAGCGC AGCACCCCCA GCCCCTTTTG AACACCGGGT AGTGAGCATC 
AAAGATACCC TGATCTCAGC AGGCTACACG GTATCCCAAC ATGAAGTCTT AGGAGGGGGT 
CGGTTTGGCC AGGTGCACAG GTGTACAGAG AGGTCTACAG GCCTTGCACT GGCAGCCAAG 
ATCATCAAAG TGAAGAACGT AAAGGACCGG GAGGATGTGA AGAATGAGGT CAACATCATG 
AACCAGCTCA GCCACGTAAA CTTGATCCAA CTTTATGATG CGTTTGAGAG CAAGAGCAGC 
TTCACTCTGA TCATGGAGTA TGTGGATGGA GGCGAACTCT TTGACCGGAT CACGGATGAG 
AAGTACCACC TCACTGAGTT GGATGTGGTC TTGTTCACGA GGCAGATCTG TGAGGGTGTG 
CATTACCTGC ATCAGCACTA TATCCTGCAC CTGGACCTCA AGCCTGAGAA CATATTGTGT 
GTCAGCCAGA CAGGGCATCA AATTAAGATC ATTGACTTTG GGCTGGCTAG AAGATACAAG 
CCTCGGGAGA AGCTAAAGGT GAACTTTGGT ACTCCGGAGT TCCTGGCCCC AGAAGTTGTT 
AACTATGAGT TTGTGTCATT TCCAACAGAC ATGTGGAGTG TGGGAGTTAT CACCTACATG 
CTACTCAGTG GTTTGTCCCC ATTTCTAGGG GAGACAGATG CAGAGACCAT GAATTTTATT 
GTGAACTGCA GCTGGGATTT CGATGCTGAT ACCTTCAAAG GGCTGTCGGA GGAAGCCAAG 
GACTTTGTTT CCCGGTTACT GGTCAAAGAG AAGAGCTGTA GGATGAGCGC CACACAGTGC 
CTGAAACACG AGTGGTTAAG TCACCTGCCT GCCAAAGCCT CGGGCTCCAA CGTTCGCCTC 
AGATCCCAAC AACTGCTGCA GAAATATATG GCTCAGAGTA AATGGAAGAA ACATTTCCAC 
GTGGTGACTG CAGTCAACAG GCTACGGAAA TTTCCAACGT GTCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk3 ( NM_175441.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780650.2 Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23765
Clone ID Related Accession (Same CDS sequence)	NM_175441.5
Accession Version	NM_175441.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC134854.3. On May 2, 2007 this sequence version replaced NM_175441.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146683.1, EU403565.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140, SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGGAG TTTCAGAGGA GGACCCAGAG GGTCTGGCCC CCCAGGGTCT GCCAGCATTG 
GGCGGAGCCT GCTTAGCCAC CATGGACAAA AAACTTAATG TGCTGACTGA GAAGGTCGAC 
AGACTCTTGC ATTTCCAAGA AGATGTCACA GAGAAGCTGC AGTGTGTGTG CCAAGGCATG 
GATCACCTGG AACAAGATCT GCATCGGCTG GAGGCCTCCC GGGAGTTGAG TCTGGCAGGG 
TCTGGCAGCA CTCCCCCAAC CACGGCTCAG GCCGCATGGC CTGAGGTCCT GGAGCTGGTG 
AGGGCTGTGC GGCAGGAGGG TGCCCAGCAT GGTGCCAGGC TCGAAGCCCT CTTCAAGATG 
GTGGTGGCTG TGGACAGGGC TATTACTTTG GTAGGGTCCA CATTCCAGAA TTCCAAGGTG 
GCTGATTTCA TCATGCAAGG AACCGTGCCC GGGAGAAAAG GCAGTCTGGC CGATGGCCCG 
GAGGAGAACA AGGAGCAAGC AGAAGTTGCT GGAGTGAAGC CAAACCATGT ACTGACTACA 
GGAGGTGTGC AAGCTGACGC CTCTAGGACG CTGTGGGAAG AGAGCCAAAA GGAGGACATA 
CCTGTGCGAA CAGTGGAGGG GCTGCCTCTC ATCATCAATA CGTCACTGAA GGGAGCTGAC 
CTAACCCAGG CAGGAGCCTC ACTGAGGCAG GGAGTTGAAG TTCTTGGCCC AGGCCAAGTA 
CCCCTACCCA CAGAAGCAGA ATCCAGGCTT CCTGAGACAG CCAGTGAGAA CACTGGAGCC 
ACCCTGGAAT TGTCCGTAGC AATCGACAGA ATCAGCGAGG TCCTCACTAG CCTCAAGATG 
TCACAAGGTG GTGGTCAAGA AACCTCATCC AGCAAGCCTG ACTGTTGGCT TTCAGAAGAG 
GCCATGAGGC TGAGTTCAGG GCCTCTCCCT CAGCCCCTAG GACCACTAAC TCCAGACAGT 
GACATTCACA GTGGTGATGC ACTTCCCAGG ATCCCTATCA ATATGCAAGA GATGGCTACT 
CCTGGGGAGT TGCTTGAGAC CCAAAGTGGC AGTCCCATTG GCTCTGCAGA AGCTCCAGGC 
CTTGGGACTG TGTTAGAAGA CCAGATCCCT AAAGGAGCCA GACCATTTCC ACCCCTGCCA 
AAGAGGAGCA GCAACAATGG TGGCATGAGT GCAGAGGAGG AGATAGGGTC TGGGGCTGAG 
CCTATGAGAG GACCAAGCTT GGCTACAAGG GACTGGAGAG ATGAGACTGT TGGGACCACA 
GACCTGCAGC AAGGCATAGA CCCAGGAGCA GTGAGCCCTG AGCCTGGGAA GGACCACGCA 
GCCCAGGGCC CAGGAAGAAC TGAAGCTGGA AGGCTATCTT CTGCTGCAGA GGCTGCCATT 
GTGGTTCTAG ATGACAGCGC AGCACCCCCA GCCCCTTTTG AACACCGGGT AGTGAGCATC 
AAAGATACCC TGATCTCAGC AGGCTACACG GTATCCCAAC ATGAAGTCTT AGGAGGGGGT 
CGGTTTGGCC AGGTGCACAG GTGTACAGAG AGGTCTACAG GCCTTGCACT GGCAGCCAAG 
ATCATCAAAG TGAAGAACGT AAAGGACCGG GAGGATGTGA AGAATGAGGT CAACATCATG 
AACCAGCTCA GCCACGTAAA CTTGATCCAA CTTTATGATG CGTTTGAGAG CAAGAGCAGC 
TTCACTCTGA TCATGGAGTA TGTGGATGGA GGCGAACTCT TTGACCGGAT CACGGATGAG 
AAGTACCACC TCACTGAGTT GGATGTGGTC TTGTTCACGA GGCAGATCTG TGAGGGTGTG 
CATTACCTGC ATCAGCACTA TATCCTGCAC CTGGACCTCA AGCCTGAGAA CATATTGTGT 
GTCAGCCAGA CAGGGCATCA AATTAAGATC ATTGACTTTG GGCTGGCTAG AAGATACAAG 
CCTCGGGAGA AGCTAAAGGT GAACTTTGGT ACTCCGGAGT TCCTGGCCCC AGAAGTTGTT 
AACTATGAGT TTGTGTCATT TCCAACAGAC ATGTGGAGTG TGGGAGTTAT CACCTACATG 
CTACTCAGTG GTTTGTCCCC ATTTCTAGGG GAGACAGATG CAGAGACCAT GAATTTTATT 
GTGAACTGCA GCTGGGATTT CGATGCTGAT ACCTTCAAAG GGCTGTCGGA GGAAGCCAAG 
GACTTTGTTT CCCGGTTACT GGTCAAAGAG AAGAGCTGTA GGATGAGCGC CACACAGTGC 
CTGAAACACG AGTGGTTAAG TCACCTGCCT GCCAAAGCCT CGGGCTCCAA CGTTCGCCTC 
AGATCCCAAC AACTGCTGCA GAAATATATG GCTCAGAGTA AATGGAAGAA ACATTTCCAC 
GTGGTGACTG CAGTCAACAG GCTACGGAAA TTTCCAACGT GTCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780650.2
CDS	151..2538
Translation	MSGVSEEDPEGLAPQGLPALGGACLATMDKKLNVLTEKVDRLLHFQEDVTEKLQCVCQGMDHLEQDLHRLEASRELSLAGSGSTPPTTAQAAWPEVLELVRAVRQEGAQHGARLEALFKMVVAVDRAITLVGSTFQNSKVADFIMQGTVPGRKGSLADGPEENKEQAEVAGVKPNHVLTTGGVQADASRTLWEESQKEDIPVRTVEGLPLIINTSLKGADLTQAGASLRQGVEVLGPGQVPLPTEAESRLPETASENTGATLELSVAIDRISEVLTSLKMSQGGGQETSSSKPDCWLSEEAMRLSSGPLPQPLGPLTPDSDIHSGDALPRIPINMQEMATPGELLETQSGSPIGSAEAPGLGTVLEDQIPKGARPFPPLPKRSSNNGGMSAEEEIGSGAEPMRGPSLATRDWRDETVGTTDLQQGIDPGAVSPEPGKDHAAQGPGRTEAGRLSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDTLISAGYTVSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNVKDREDVKNEVNIMNQLSHVNLIQLYDAFESKSSFTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQTGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLSHLPAKASGSNVRLRSQQLLQKYMAQSKWKKHFHVVTAVNRLRKFPTCP

Target ORF information:

RefSeq Version	NM_175441.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175441.5

ORF Insert Sequence:

ATGTCAGGAG TTTCAGAGGA GGACCCAGAG GGTCTGGCCC CCCAGGGTCT GCCAGCATTG 
GGCGGAGCCT GCTTAGCCAC CATGGACAAA AAACTTAATG TGCTGACTGA GAAGGTCGAC 
AGACTCTTGC ATTTCCAAGA AGATGTCACA GAGAAGCTGC AGTGTGTGTG CCAAGGCATG 
GATCACCTGG AACAAGATCT GCATCGGCTG GAGGCCTCCC GGGAGTTGAG TCTGGCAGGG 
TCTGGCAGCA CTCCCCCAAC CACGGCTCAG GCCGCATGGC CTGAGGTCCT GGAGCTGGTG 
AGGGCTGTGC GGCAGGAGGG TGCCCAGCAT GGTGCCAGGC TCGAAGCCCT CTTCAAGATG 
GTGGTGGCTG TGGACAGGGC TATTACTTTG GTAGGGTCCA CATTCCAGAA TTCCAAGGTG 
GCTGATTTCA TCATGCAAGG AACCGTGCCC GGGAGAAAAG GCAGTCTGGC CGATGGCCCG 
GAGGAGAACA AGGAGCAAGC AGAAGTTGCT GGAGTGAAGC CAAACCATGT ACTGACTACA 
GGAGGTGTGC AAGCTGACGC CTCTAGGACG CTGTGGGAAG AGAGCCAAAA GGAGGACATA 
CCTGTGCGAA CAGTGGAGGG GCTGCCTCTC ATCATCAATA CGTCACTGAA GGGAGCTGAC 
CTAACCCAGG CAGGAGCCTC ACTGAGGCAG GGAGTTGAAG TTCTTGGCCC AGGCCAAGTA 
CCCCTACCCA CAGAAGCAGA ATCCAGGCTT CCTGAGACAG CCAGTGAGAA CACTGGAGCC 
ACCCTGGAAT TGTCCGTAGC AATCGACAGA ATCAGCGAGG TCCTCACTAG CCTCAAGATG 
TCACAAGGTG GTGGTCAAGA AACCTCATCC AGCAAGCCTG ACTGTTGGCT TTCAGAAGAG 
GCCATGAGGC TGAGTTCAGG GCCTCTCCCT CAGCCCCTAG GACCACTAAC TCCAGACAGT 
GACATTCACA GTGGTGATGC ACTTCCCAGG ATCCCTATCA ATATGCAAGA GATGGCTACT 
CCTGGGGAGT TGCTTGAGAC CCAAAGTGGC AGTCCCATTG GCTCTGCAGA AGCTCCAGGC 
CTTGGGACTG TGTTAGAAGA CCAGATCCCT AAAGGAGCCA GACCATTTCC ACCCCTGCCA 
AAGAGGAGCA GCAACAATGG TGGCATGAGT GCAGAGGAGG AGATAGGGTC TGGGGCTGAG 
CCTATGAGAG GACCAAGCTT GGCTACAAGG GACTGGAGAG ATGAGACTGT TGGGACCACA 
GACCTGCAGC AAGGCATAGA CCCAGGAGCA GTGAGCCCTG AGCCTGGGAA GGACCACGCA 
GCCCAGGGCC CAGGAAGAAC TGAAGCTGGA AGGCTATCTT CTGCTGCAGA GGCTGCCATT 
GTGGTTCTAG ATGACAGCGC AGCACCCCCA GCCCCTTTTG AACACCGGGT AGTGAGCATC 
AAAGATACCC TGATCTCAGC AGGCTACACG GTATCCCAAC ATGAAGTCTT AGGAGGGGGT 
CGGTTTGGCC AGGTGCACAG GTGTACAGAG AGGTCTACAG GCCTTGCACT GGCAGCCAAG 
ATCATCAAAG TGAAGAACGT AAAGGACCGG GAGGATGTGA AGAATGAGGT CAACATCATG 
AACCAGCTCA GCCACGTAAA CTTGATCCAA CTTTATGATG CGTTTGAGAG CAAGAGCAGC 
TTCACTCTGA TCATGGAGTA TGTGGATGGA GGCGAACTCT TTGACCGGAT CACGGATGAG 
AAGTACCACC TCACTGAGTT GGATGTGGTC TTGTTCACGA GGCAGATCTG TGAGGGTGTG 
CATTACCTGC ATCAGCACTA TATCCTGCAC CTGGACCTCA AGCCTGAGAA CATATTGTGT 
GTCAGCCAGA CAGGGCATCA AATTAAGATC ATTGACTTTG GGCTGGCTAG AAGATACAAG 
CCTCGGGAGA AGCTAAAGGT GAACTTTGGT ACTCCGGAGT TCCTGGCCCC AGAAGTTGTT 
AACTATGAGT TTGTGTCATT TCCAACAGAC ATGTGGAGTG TGGGAGTTAT CACCTACATG 
CTACTCAGTG GTTTGTCCCC ATTTCTAGGG GAGACAGATG CAGAGACCAT GAATTTTATT 
GTGAACTGCA GCTGGGATTT CGATGCTGAT ACCTTCAAAG GGCTGTCGGA GGAAGCCAAG 
GACTTTGTTT CCCGGTTACT GGTCAAAGAG AAGAGCTGTA GGATGAGCGC CACACAGTGC 
CTGAAACACG AGTGGTTAAG TCACCTGCCT GCCAAAGCCT CGGGCTCCAA CGTTCGCCTC 
AGATCCCAAC AACTGCTGCA GAAATATATG GCTCAGAGTA AATGGAAGAA ACATTTCCAC 
GTGGTGACTG CAGTCAACAG GCTACGGAAA TTTCCAACGT GTCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk3 ( XM_006530825.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530888.1 Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant X1, mRNA.

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CloneID	OMu41328
Clone ID Related Accession (Same CDS sequence)	XM_006530825.2 , XM_006530825.3
Accession Version	XM_006530825.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin light chain kinase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530825.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACA AGGAGCAAGC AGAAGTTGCT GGAGTGAAGC CAAACCATGT ACTGACTACA 
GGAGGTGTGC AAGCTGACGC CTCTAGGACG CTGTGGGAAG AGAGCCAAAA GGAGGACATA 
CCTGTGCGAA CAGTGGAGGG GCTGCCTCTC ATCATCAATA CGTCACTGAA GGGAGCTGAC 
CTAACCCAGG CAGGAGCCTC ACTGAGGCAG GGAGTTGAAG TTCTTGGCCC AGGCCAAGTA 
CCCCTACCCA CAGAAGCAGA ATCCAGGCTT CCTGAGACAG CCAGTGAGAA CACTGGAGCC 
ACCCTGGAAT TGTCCGTAGC AATCGACAGA ATCAGCGAGG TCCTCACTAG CCTCAAGATG 
TCACAAGGTG GTGGTCAAGA AACCTCATCC AGCAAGCCTG ACTGTTGGCT TTCAGAAGAG 
GCCATGAGGC TGAGTTCAGG GCCTCTCCCT CAGCCCCTAG GACCACTAAC TCCAGACAGT 
GACATTCACA GTGGTGATGC ACTTCCCAGG ATCCCTATCA ATATGCAAGA GATGGCTACT 
CCTGGGGAGT TGCTTGAGAC CCAAAGTGGC AGTCCCATTG GCTCTGCAGA AGCTCCAGGC 
CTTGGGACTG TGTTAGAAGA CCAGATCCCT AAAGGAGCCA GACCATTTCC ACCCCTGCCA 
AAGAGGAGCA GCAACAATGG TGGCATGAGT GCAGAGGAGG AGATAGGGTC TGGGGCTGAG 
CCTATGAGAG GACCAAGCTT GGCTACAAGG GACTGGAGAG ATGAGACTGT TGGGACCACA 
GACCTGCAGC AAGGCATAGA CCCAGGAGCA GTGAGCCCTG AGCCTGGGAA GGACCACGCA 
GCCCAGGGCC CAGGAAGAAC TGAAGCTGGA AGGCTATCTT CTGCTGCAGA GGCTGCCATT 
GTGGTTCTAG ATGACAGCGC AGCACCCCCA GCCCCTTTTG AACACCGGGT AGTGAGCATC 
AAAGATACCC TGATCTCAGC AGGCTACACG GTATCCCAAC ATGAAGTCTT AGGAGGGGGT 
CGGTTTGGCC AGGTGCACAG GTGTACAGAG AGGTCTACAG GCCTTGCACT GGCAGCCAAG 
ATCATCAAAG TGAAGAACGT AAAGGACCGG GAGGATGTGA AGAATGAGGT CAACATCATG 
AACCAGCTCA GCCACGTAAA CTTGATCCAA CTTTATGATG CGTTTGAGAG CAAGAGCAGC 
TTCACTCTGA TCATGGAGTA TGTGGATGGA GGCGAACTCT TTGACCGGAT CACGGATGAG 
AAGTACCACC TCACTGAGTT GGATGTGGTC TTGTTCACGA GGCAGATCTG TGAGGGTGTG 
CATTACCTGC ATCAGCACTA TATCCTGCAC CTGGACCTCA AGCCTGAGAA CATATTGTGT 
GTCAGCCAGA CAGGGCATCA AATTAAGATC ATTGACTTTG GGCTGGCTAG AAGATACAAG 
CCTCGGGAGA AGCTAAAGGT GAACTTTGGT ACTCCGGAGT TCCTGGCCCC AGAAGTTGTT 
AACTATGAGT TTGTGTCATT TCCAACAGAC ATGTGGAGTG TGGGAGTTAT CACCTACATG 
CTACTCAGTG GTTTGTCCCC ATTTCTAGGG GAGACAGATG CAGAGACCAT GAATTTTATT 
GTGAACTGCA GCTGGGATTT CGATGCTGAT ACCTTCAAAG GGCTGTCGGA GGAAGCCAAG 
GACTTTGTTT CCCGGTTACT GGTCAAAGAG AAGAGCTGTA GGATGAGCGC CACACAGTGC 
CTGAAACACG AGTGGTTAAG TCACCTGCCT GCCAAAGCCT CGGGCTCCAA CGTTCGCCTC 
AGATCCCAAC AACTGCTGCA GAAATATATG GCTCAGAGTA AATGGAAGAA ACATTTCCAC 
GTGGTGACTG CAGTCAACAG GCTACGGAAA TTTCCAACGT GTCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530888.1
CDS	228..2135
Translation	MKNKEQAEVAGVKPNHVLTTGGVQADASRTLWEESQKEDIPVRTVEGLPLIINTSLKGADLTQAGASLRQGVEVLGPGQVPLPTEAESRLPETASENTGATLELSVAIDRISEVLTSLKMSQGGGQETSSSKPDCWLSEEAMRLSSGPLPQPLGPLTPDSDIHSGDALPRIPINMQEMATPGELLETQSGSPIGSAEAPGLGTVLEDQIPKGARPFPPLPKRSSNNGGMSAEEEIGSGAEPMRGPSLATRDWRDETVGTTDLQQGIDPGAVSPEPGKDHAAQGPGRTEAGRLSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDTLISAGYTVSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNVKDREDVKNEVNIMNQLSHVNLIQLYDAFESKSSFTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQTGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLSHLPAKASGSNVRLRSQQLLQKYMAQSKWKKHFHVVTAVNRLRKFPTCP

Target ORF information:

RefSeq Version	XM_006530825.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain kinase 3 (Mylk3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530825.3

ORF Insert Sequence:

ATGAAGAACA AGGAGCAAGC AGAAGTTGCT GGAGTGAAGC CAAACCATGT ACTGACTACA 
GGAGGTGTGC AAGCTGACGC CTCTAGGACG CTGTGGGAAG AGAGCCAAAA GGAGGACATA 
CCTGTGCGAA CAGTGGAGGG GCTGCCTCTC ATCATCAATA CGTCACTGAA GGGAGCTGAC 
CTAACCCAGG CAGGAGCCTC ACTGAGGCAG GGAGTTGAAG TTCTTGGCCC AGGCCAAGTA 
CCCCTACCCA CAGAAGCAGA ATCCAGGCTT CCTGAGACAG CCAGTGAGAA CACTGGAGCC 
ACCCTGGAAT TGTCCGTAGC AATCGACAGA ATCAGCGAGG TCCTCACTAG CCTCAAGATG 
TCACAAGGTG GTGGTCAAGA AACCTCATCC AGCAAGCCTG ACTGTTGGCT TTCAGAAGAG 
GCCATGAGGC TGAGTTCAGG GCCTCTCCCT CAGCCCCTAG GACCACTAAC TCCAGACAGT 
GACATTCACA GTGGTGATGC ACTTCCCAGG ATCCCTATCA ATATGCAAGA GATGGCTACT 
CCTGGGGAGT TGCTTGAGAC CCAAAGTGGC AGTCCCATTG GCTCTGCAGA AGCTCCAGGC 
CTTGGGACTG TGTTAGAAGA CCAGATCCCT AAAGGAGCCA GACCATTTCC ACCCCTGCCA 
AAGAGGAGCA GCAACAATGG TGGCATGAGT GCAGAGGAGG AGATAGGGTC TGGGGCTGAG 
CCTATGAGAG GACCAAGCTT GGCTACAAGG GACTGGAGAG ATGAGACTGT TGGGACCACA 
GACCTGCAGC AAGGCATAGA CCCAGGAGCA GTGAGCCCTG AGCCTGGGAA GGACCACGCA 
GCCCAGGGCC CAGGAAGAAC TGAAGCTGGA AGGCTATCTT CTGCTGCAGA GGCTGCCATT 
GTGGTTCTAG ATGACAGCGC AGCACCCCCA GCCCCTTTTG AACACCGGGT AGTGAGCATC 
AAAGATACCC TGATCTCAGC AGGCTACACG GTATCCCAAC ATGAAGTCTT AGGAGGGGGT 
CGGTTTGGCC AGGTGCACAG GTGTACAGAG AGGTCTACAG GCCTTGCACT GGCAGCCAAG 
ATCATCAAAG TGAAGAACGT AAAGGACCGG GAGGATGTGA AGAATGAGGT CAACATCATG 
AACCAGCTCA GCCACGTAAA CTTGATCCAA CTTTATGATG CGTTTGAGAG CAAGAGCAGC 
TTCACTCTGA TCATGGAGTA TGTGGATGGA GGCGAACTCT TTGACCGGAT CACGGATGAG 
AAGTACCACC TCACTGAGTT GGATGTGGTC TTGTTCACGA GGCAGATCTG TGAGGGTGTG 
CATTACCTGC ATCAGCACTA TATCCTGCAC CTGGACCTCA AGCCTGAGAA CATATTGTGT 
GTCAGCCAGA CAGGGCATCA AATTAAGATC ATTGACTTTG GGCTGGCTAG AAGATACAAG 
CCTCGGGAGA AGCTAAAGGT GAACTTTGGT ACTCCGGAGT TCCTGGCCCC AGAAGTTGTT 
AACTATGAGT TTGTGTCATT TCCAACAGAC ATGTGGAGTG TGGGAGTTAT CACCTACATG 
CTACTCAGTG GTTTGTCCCC ATTTCTAGGG GAGACAGATG CAGAGACCAT GAATTTTATT 
GTGAACTGCA GCTGGGATTT CGATGCTGAT ACCTTCAAAG GGCTGTCGGA GGAAGCCAAG 
GACTTTGTTT CCCGGTTACT GGTCAAAGAG AAGAGCTGTA GGATGAGCGC CACACAGTGC 
CTGAAACACG AGTGGTTAAG TCACCTGCCT GCCAAAGCCT CGGGCTCCAA CGTTCGCCTC 
AGATCCCAAC AACTGCTGCA GAAATATATG GCTCAGAGTA AATGGAAGAA ACATTTCCAC 
GTGGTGACTG CAGTCAACAG GCTACGGAAA TTTCCAACGT GTCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation.
Scientific reports7(1)11533(2017 Sep)
Song KH,Lee J,Jung HR,Park H,Doh J

World journal of gastroenterology23(12)2175-2184(2017 Mar)
Han J,Li JH,Bai G,Shen GS,Chen J,Liu JN,Wang S,Liu XJ

Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments.
Nature cell biology19(2)85-93(2017 Feb)
Beach JR,Bruun KS,Shao L,Li D,Swider Z,Remmert K,Zhang Y,Conti MA,Adelstein RS,Rusan NM,Betzig E,Hammer JA

Cardiac myosin light chain is phosphorylated by Ca2+/calmodulin-dependent and -independent kinase activities.
Proceedings of the National Academy of Sciences of the United States of America113(27)E3824-33(2016 07)
Chang AN,Mahajan P,Knapp S,Barton H,Sweeney HL,Kamm KE,Stull JT

The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.
Cell metabolism23(5)881-92(2016 May)
G?mez-Del Arco P,Perdiguero E,Yunes-Leites PS,Ac?n-P?rez R,Zeini M,Garcia-Gomez A,Sreenivasan K,Jim?nez-Alc?zar M,Segal?s J,L?pez-Maderuelo D,Orn?s B,Jim?nez-Borreguero LJ,D'Amato G,Enshell-Seijffers D,Morgan B,Georgopoulos K,Islam AB,Braun T,de la Pompa JL,Kim J,Enriquez JA,Ballestar E,Mu?oz-C?noves P,Redondo JM

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Mylk3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

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電話

FAX

オンラインフォーム