Mylk3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mylk3
Entrez Gene ID	291926
Full Name	myosin light chain kinase 3
Synonyms	MLCK,RGD1305801
General protein information	Preferred Names myosin light chain kinase 3 Names myosin light chain kinase 3 cardiac-MLCK cardiac-MyBP-C-associated Ca/CaM kinase putative myosin light chain kinase 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255355.2	XP_006255417.1	myosin light chain kinase 3 isoform X1
NM_001110810.1	NP_001104280.1	myosin light chain kinase 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYLK3	XP_003315132.1
	MYLK3	XP_001113601.1
Canis lupus familiaris (dog)	MYLK3	XP_532569.4
Bos taurus (cattle)	MYLK3	XP_005218847.1
Mus musculus (house mouse)	Mylk3	NP_780650.2
Homo sapiens (human)	MYLK3	NP_872299.2
Rattus norvegicus (Norway rat)	Mylk3	NP_001104280.1
Gallus gallus (chicken)	MYLK3	XP_004944251.1
Xenopus tropicalis (tropical clawed frog)	mylk3	XP_002931740.2

Gene Ontology
Bibliography

Related articles in PubMed

The shift in GH3 cell shape and cell motility is dependent on MLCK and ROCK.
?vila-Rodr?guez D, Solano Agama C, Gonz?lez-Pozos S, Vicente M?ndez-M?ndez J, Ortiz Plata A, Arreola-Mendoza L, Mendoza-Garrido ME
Experimental cell research354(1)1-17(2017 05)

CaMKII in addition to MLCK contributes to phosphorylation of regulatory light chain in cardiomyocytes.
Eikemo H, Moltzau LR, Hussain RI, Nguyen CH, Qvigstad E, Levy FO, Skomedal T, Osnes JB
Biochemical and biophysical research communications471(1)219-25(2016 Feb)

Degradation of cardiac myosin light chain kinase by matrix metalloproteinase-2 contributes to myocardial contractile dysfunction during ischemia/reperfusion.
Gao L, Zheng YJ, Gu SS, Tan JL, Paul C, Wang YG, Yang HT
Journal of molecular and cellular cardiology77102-12(2014 Dec)

Mechanism of IL-1beta-induced increase in intestinal epithelial tight junction permeability.
Al-Sadi R, Ye D, Dokladny K, Ma TY
Journal of immunology (Baltimore, Md. : 1950)180(8)5653-61(2008 Apr)

Identification of cardiac-specific myosin light chain kinase.
Chan JY, Takeda M, Briggs LE, Graham ML, Lu JT, Horikoshi N, Weinberg EO, Aoki H, Sato N, Chien KR, Kasahara H
Circulation research102(5)571-80(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that a rounded shape with high cortical tension depends on MLCK and ROCK, whereas in cells with an elongated shape, MLCK is the primary protein responsible for cell spreading. Further, in cells with a slow and directionally persistent motility, MLCK predominates, while rapid and erratic movement is ROCK-dependent
Title: The shift in GH3 cell shape and cell motility is dependent on MLCK and ROCK.

In conclusion, in addition to MLCK, CaMKII phosphorylates RLC in cardiomyocytes.
Title: CaMKII in addition to MLCK contributes to phosphorylation of regulatory light chain in cardiomyocytes.

Ischemia/reperfusion-activated MMP-2 leads to the degradation of cardiac MLCK.
Title: Degradation of cardiac myosin light chain kinase by matrix metalloproteinase-2 contributes to myocardial contractile dysfunction during ischemia/reperfusion.

The following Mylk3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mylk3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00854	NM_001110810.1 Latest version!	Rattus norvegicus myosin light chain kinase 3 (Mylk3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa38761	XM_006255355.2 Latest version!	Rattus norvegicus myosin light chain kinase 3 (Mylk3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mylk3 ( NM_001110810.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001104280.1 Rattus norvegicus myosin light chain kinase 3 (Mylk3), mRNA.

Price & Availability↑

CloneID	ORa00854
Clone ID Related Accession (Same CDS sequence)	NM_001110810.1
Accession Version	NM_001110810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin light chain kinase 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU236721.1. On or before Nov 24, 2007 this sequence version replaced XM_001064628.1, XM_214645.4. ##Evidence-Data-START## Transcript exon combination :: EU236721.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689600 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGGAG TTTCAGAGGA GGATCCAGAG GGTCTGGGCC CCCAGGGTCT GCCAGCGTTG 
GGCGGAGCCT GCTTAGTCAC CGTGGACAAA AAACTTAATG TGCTGACTGA GAAGGTCGAC 
AGACTCTTGC ATTTCCAAGA AGATGTCACA GAGAAGCTAC AGTGTGTGTG CCAAGGCATG 
GATCACCTGG AACAAGGTCT GCATCGGCTG GAGGCCTCCC AGGAGTTGGG TCTGGCAGGG 
CCCGGCAGCA CTTCCCCAGC CGCTGCTCAG GCCGCATGGC CTGAGGTCCT GGAGCTGGTG 
AGGGCCGTGC GGCAGGAGGG TGCCCAGCAC GGTGCCAGGC TCGAAGCCCT CTTCAAGATG 
GTGGTGGCTG TGGACAGGGC TATTACTTTG GTAGGGTCCA CAATCCAGAA CTCCAAAGTG 
GATGATTTCA TCCTGCAAGG GACCGTGCCC TGGAGGAAAG GCAGTCTGGC TGATGGCCCT 
GAGGAGAACA AGGAGCAAGC AGAAGTGGCT GGAGTGAAGC CAAAGCATGT GCTGAATACA 
GGAAGTGTGC AAGCTGCCAC TTCTAGGGCG CTGTGGGAAG AGAGCCAGAA GCAGGACACA 
CCCGTGGGGA CAGTGGAGGG GCTGCCTCTC ATCATCGATA CGTCACTGAA GGGAGCTGAC 
CTCACCCAGG CTGGAGCCTC ACTGAGGCAG GGAGTTGAAG CTCTTGACCC AGGCCAAGAA 
CCCCCACCCG CAGAGGCAGA ATCCAGGCTT CCTGCACTAG CCAGCGAGGA CACTGGGACC 
ACCCTGGAAT TGTCTGTAGC AATTGACAGA ATCAGTGAGG TCCTCACTAG CCTCAGGATG 
TCCCAAAGTG CTGGCGAAGG AACCTCATCC AGCAAGCCTG ACTGTTCAGA GCCTGGCCCT 
CAGCCCCTAG GGCCACTAAC TACAGACAGT GACATTCACA GTGATGAAGG ACTTCCCAGG 
ATCTCTGTCC GTATGCGAGA GATGACTACT CCTGAGGAGC TGTTTGAGAC CCAAGGTGGC 
AGCCCCATTG GCTCGGCAGA AGCTCCAGGC CCTGGAACTG TGTTAGAAGA CCAGATCCCT 
AAAGGAGCCA GACCATTTCC ACCCCTGCCA AAGAGGAGCT GCAACAATGG TGGCGCGAGT 
GCAGAGGAGG CAACAGGGCC TGGGGCTGAG CCCATCAGAG GACCAAGCTT GGTCACAAGG 
GACTGGAGAG ATGAACCTGT TGGGACCACA GACCTGCAGC AAGGCAGAGA CCCAGGAGCG 
GTGAGCCCTG AACCTGGGAA GGACCATGCA GCCCAGGGCC CAGGGAGAAC CGAAGCTGGA 
AGGAGGGTGT CTTCTGCTGC AGAGGCTGCC ATCGTAGTTC TAGATGACAG CGCAGCACCC 
CCAGCCCCTT TTGAACACCG GGTAGTGAGC ATCAAAGACA CCCTCATCTC GACAAGTTAC 
ACAGTGTCCC AACACGAAGT CTTGGGAGGG GGCCGGTTTG GCCAGGTGCA CAGGTGTACA 
GAGCGGTCCA CAGGCCTTGC ACTGGCAGCC AAGATCATCA AAGTGAAGAA CATAAAGGAC 
CGGGAGGATG TGAAGAATGA GATCAACATC ATGAACCAGC TCAGCCACGT AAACTTGATC 
CAACTTTATG ATGCTTTTGA GAGCAAGAAC AGCTTCACCC TGATCATGGA GTATGTGGAT 
GGAGGTGAAC TCTTCGACCG GATCACGGAT GAGAAGTACC ACCTGACCGA GCTGGATGTG 
GTCTTGTTCA CAAGGCAGAT CTGTGAGGGT GTGCATTACC TGCACCAGCA CTACATTCTG 
CACCTGGACC TCAAGCCAGA GAACATACTG TGTGTCAGCC AGACTGGGCA TCAAATTAAG 
ATTATTGACT TTGGGCTGGC TAGAAGATAT AAACCTCGGG AGAAGCTAAA GGTTAACTTT 
GGTACTCCAG AGTTCCTGGC TCCAGAAGTT GTTAACTATG AGTTTGTCTC ATTCCCAACA 
GACATGTGGA GTGTGGGAGT TATCACCTAC ATGCTACTCA GTGGTTTGTC CCCATTTCTA 
GGGGAAACAG ATGCAGAGAC CATGAATTTT ATTGTGAACT GCAGCTGGGA TTTCGATGCT 
GATACCTTCA AAGGGCTGTC AGAGGAAGCC AAGGACTTTG TTTCACGGTT GCTGGTCAAA 
GAGAAGAGTT GTAGGATGAG CGCCACACAG TGCCTGAAAC ATGAGTGGTT AAATCACCTG 
ATTGCCAAAG CCTCAGGCTC CAACGTTCGC CTCAGATCCC AACTACTGCT GCAGAAATAT 
ATGGCTCAGC GTAAATGGAA GAAACATTTC CATGTGGTGA CTGCAGTCAA CAGGCTAAGA 
AAATTTCCAA CGTGTCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104280.1
CDS	238..2598
Translation	MSGVSEEDPEGLGPQGLPALGGACLVTVDKKLNVLTEKVDRLLHFQEDVTEKLQCVCQGMDHLEQGLHRLEASQELGLAGPGSTSPAAAQAAWPEVLELVRAVRQEGAQHGARLEALFKMVVAVDRAITLVGSTIQNSKVDDFILQGTVPWRKGSLADGPEENKEQAEVAGVKPKHVLNTGSVQAATSRALWEESQKQDTPVGTVEGLPLIIDTSLKGADLTQAGASLRQGVEALDPGQEPPPAEAESRLPALASEDTGTTLELSVAIDRISEVLTSLRMSQSAGEGTSSSKPDCSEPGPQPLGPLTTDSDIHSDEGLPRISVRMREMTTPEELFETQGGSPIGSAEAPGPGTVLEDQIPKGARPFPPLPKRSCNNGGASAEEATGPGAEPIRGPSLVTRDWRDEPVGTTDLQQGRDPGAVSPEPGKDHAAQGPGRTEAGRRVSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDTLISTSYTVSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNIKDREDVKNEINIMNQLSHVNLIQLYDAFESKNSFTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQTGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLNHLIAKASGSNVRLRSQLLLQKYMAQRKWKKHFHVVTAVNRLRKFPTCP

Target ORF information:

RefSeq Version	NM_001110810.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin light chain kinase 3 (Mylk3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110810.1

ORF Insert Sequence:

ATGTCAGGAG TTTCAGAGGA GGATCCAGAG GGTCTGGGCC CCCAGGGTCT GCCAGCGTTG 
GGCGGAGCCT GCTTAGTCAC CGTGGACAAA AAACTTAATG TGCTGACTGA GAAGGTCGAC 
AGACTCTTGC ATTTCCAAGA AGATGTCACA GAGAAGCTAC AGTGTGTGTG CCAAGGCATG 
GATCACCTGG AACAAGGTCT GCATCGGCTG GAGGCCTCCC AGGAGTTGGG TCTGGCAGGG 
CCCGGCAGCA CTTCCCCAGC CGCTGCTCAG GCCGCATGGC CTGAGGTCCT GGAGCTGGTG 
AGGGCCGTGC GGCAGGAGGG TGCCCAGCAC GGTGCCAGGC TCGAAGCCCT CTTCAAGATG 
GTGGTGGCTG TGGACAGGGC TATTACTTTG GTAGGGTCCA CAATCCAGAA CTCCAAAGTG 
GATGATTTCA TCCTGCAAGG GACCGTGCCC TGGAGGAAAG GCAGTCTGGC TGATGGCCCT 
GAGGAGAACA AGGAGCAAGC AGAAGTGGCT GGAGTGAAGC CAAAGCATGT GCTGAATACA 
GGAAGTGTGC AAGCTGCCAC TTCTAGGGCG CTGTGGGAAG AGAGCCAGAA GCAGGACACA 
CCCGTGGGGA CAGTGGAGGG GCTGCCTCTC ATCATCGATA CGTCACTGAA GGGAGCTGAC 
CTCACCCAGG CTGGAGCCTC ACTGAGGCAG GGAGTTGAAG CTCTTGACCC AGGCCAAGAA 
CCCCCACCCG CAGAGGCAGA ATCCAGGCTT CCTGCACTAG CCAGCGAGGA CACTGGGACC 
ACCCTGGAAT TGTCTGTAGC AATTGACAGA ATCAGTGAGG TCCTCACTAG CCTCAGGATG 
TCCCAAAGTG CTGGCGAAGG AACCTCATCC AGCAAGCCTG ACTGTTCAGA GCCTGGCCCT 
CAGCCCCTAG GGCCACTAAC TACAGACAGT GACATTCACA GTGATGAAGG ACTTCCCAGG 
ATCTCTGTCC GTATGCGAGA GATGACTACT CCTGAGGAGC TGTTTGAGAC CCAAGGTGGC 
AGCCCCATTG GCTCGGCAGA AGCTCCAGGC CCTGGAACTG TGTTAGAAGA CCAGATCCCT 
AAAGGAGCCA GACCATTTCC ACCCCTGCCA AAGAGGAGCT GCAACAATGG TGGCGCGAGT 
GCAGAGGAGG CAACAGGGCC TGGGGCTGAG CCCATCAGAG GACCAAGCTT GGTCACAAGG 
GACTGGAGAG ATGAACCTGT TGGGACCACA GACCTGCAGC AAGGCAGAGA CCCAGGAGCG 
GTGAGCCCTG AACCTGGGAA GGACCATGCA GCCCAGGGCC CAGGGAGAAC CGAAGCTGGA 
AGGAGGGTGT CTTCTGCTGC AGAGGCTGCC ATCGTAGTTC TAGATGACAG CGCAGCACCC 
CCAGCCCCTT TTGAACACCG GGTAGTGAGC ATCAAAGACA CCCTCATCTC GACAAGTTAC 
ACAGTGTCCC AACACGAAGT CTTGGGAGGG GGCCGGTTTG GCCAGGTGCA CAGGTGTACA 
GAGCGGTCCA CAGGCCTTGC ACTGGCAGCC AAGATCATCA AAGTGAAGAA CATAAAGGAC 
CGGGAGGATG TGAAGAATGA GATCAACATC ATGAACCAGC TCAGCCACGT AAACTTGATC 
CAACTTTATG ATGCTTTTGA GAGCAAGAAC AGCTTCACCC TGATCATGGA GTATGTGGAT 
GGAGGTGAAC TCTTCGACCG GATCACGGAT GAGAAGTACC ACCTGACCGA GCTGGATGTG 
GTCTTGTTCA CAAGGCAGAT CTGTGAGGGT GTGCATTACC TGCACCAGCA CTACATTCTG 
CACCTGGACC TCAAGCCAGA GAACATACTG TGTGTCAGCC AGACTGGGCA TCAAATTAAG 
ATTATTGACT TTGGGCTGGC TAGAAGATAT AAACCTCGGG AGAAGCTAAA GGTTAACTTT 
GGTACTCCAG AGTTCCTGGC TCCAGAAGTT GTTAACTATG AGTTTGTCTC ATTCCCAACA 
GACATGTGGA GTGTGGGAGT TATCACCTAC ATGCTACTCA GTGGTTTGTC CCCATTTCTA 
GGGGAAACAG ATGCAGAGAC CATGAATTTT ATTGTGAACT GCAGCTGGGA TTTCGATGCT 
GATACCTTCA AAGGGCTGTC AGAGGAAGCC AAGGACTTTG TTTCACGGTT GCTGGTCAAA 
GAGAAGAGTT GTAGGATGAG CGCCACACAG TGCCTGAAAC ATGAGTGGTT AAATCACCTG 
ATTGCCAAAG CCTCAGGCTC CAACGTTCGC CTCAGATCCC AACTACTGCT GCAGAAATAT 
ATGGCTCAGC GTAAATGGAA GAAACATTTC CATGTGGTGA CTGCAGTCAA CAGGCTAAGA 
AAATTTCCAA CGTGTCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylk3 ( XM_006255355.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255417.1 Rattus norvegicus myosin light chain kinase 3 (Mylk3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38761
Clone ID Related Accession (Same CDS sequence)	XM_006255355.2
Accession Version	XM_006255355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin light chain kinase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006255355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACTC TGTGCCCTGT CCCAGCTCTG TGCAGGCCCA GCACCCGAGG CCAGCAGCGG 
GCATCAGATT ACGCCCAGAT ATTCACGAAC AAGGAGCAAG CAGAAGTGGC TGGAGTGAAG 
CCAAAGCATG TGCTGAATAC AGGAAGTGTG CAAGCTGCCA CTTCTAGGGC GCTGTGGGAA 
GAGAGCCAGA AGCAGGACAC ACCCGTGGGG ACAGTGGAGG GGCTGCCTCT CATCATCGAT 
ACGTCACTGA AGGGAGCTGA CCTCACCCAG GCTGGAGCCT CACTGAGGCA GGGAGTTGAA 
GCTCTTGACC CAGGCCAAGA ACCCCCACCC ACAGAGGCAG AATCCAGGCT TCCTGCACTA 
GCCAGCGAGG ACACTGGGAC CACCCTGGAA TTGTCTGTAG CAATTGACAG AATCAGTGAG 
GTCCTCACTA GCCTCAGGAT GTCCCAAAGT GCTGGCGAAG GAACCTCATC CAGCAAGCCT 
GACTGTTCAG AGCCTGGCCC TCAGCCCCTA GGGCCACTAA CTACAGACAG TGACATTCAC 
AGTGATGAAG GACTTCCCAG GATCTCTGTC CGTATGCGAG AGATGACTAC TCCTGAGGAG 
CTGTTTGAGA CCCAAGGTGG CAGCCCCATT GGCTCGGCAG AAGCTCCAGG CCCTGGAACT 
GTGTTAGAAG ACCAGATCCC TAAAGGAGCC AGACCATTTC CACCCCTGCC AAAGAGGAGC 
TGCAACAATG GTGGCGCGAG TGCAGAGGAG GCAACAGGGC CTGGGGCTGA GCCCATCAGA 
GGACCAAGCT TGGTCACAAG GGACTGGAGA GATGAACCTG TTGGGACCAC AGACCTGCAG 
CAAGGCAGAG ACCCAGGAGC GGTGAGCCCT GAACCTGGGA AGGACCATGC AGCCCAGGGC 
CCAGGGAGAA CCGAAGCTGG AAGGAGGGTG TCTTCTGCTG CAGAGGCTGC CATCGTAGTT 
CTAGATGACA GCGCAGCACC CCCAGCCCCT TTTGAACACC GGGTAGTGAG CATCAAAGAC 
ACCCTCATCT CGACAAGTTA CACAGTGTCC CAACACGAAG TCTTGGGAGG GGGCCGGTTT 
GGCCAGGTGC ACAGGTGTAC AGAGCGGTCC ACAGGCCTTG CACTGGCAGC CAAGATCATC 
AAAGTGAAGA ACATAAAGGA CCGGGAGGAT GTGAAGAATG AGATCAACAT CATGAACCAG 
CTCAGCCACG TAAACTTGAT CCAACTTTAT GATGCTTTTG AGAGCAAGAA CAGCTTCACC 
CTGATCATGG AGTATGTGGA TGGAGGTGAA CTCTTCGACC GGATCACGGA TGAGAAGTAC 
CACCTGACCG AGCTGGATGT GGTCTTGTTC ACAAGGCAGA TCTGTGAGGG TGTGCATTAC 
CTGCACCAGC ACTACATTCT GCACCTGGAC CTCAAGCCAG AGAACATACT GTGTGTCAGC 
CAGACTGGGC ATCAAATTAA GATTATTGAC TTTGGGCTGG CTAGAAGATA TAAACCTCGG 
GAGAAGCTAA AGGTTAACTT TGGTACTCCA GAGTTCCTGG CTCCAGAAGT TGTTAACTAT 
GAGTTTGTCT CATTCCCAAC AGACATGTGG AGTGTGGGAG TTATCACCTA CATGCTACTC 
AGTGGTTTGT CCCCATTTCT AGGGGAAACA GATGCAGAGA CCATGAATTT TATTGTGAAC 
TGCAGCTGGG ATTTCGATGC TGATACCTTC AAAGGGCTGT CAGAGGAAGC CAAGGACTTT 
GTTTCACGGT TGCTGGTCAA AGAGAAGAGT TGTAGGATGA GCGCCACACA GTGCCTGAAA 
CATGAGTGGT TAAATCACCT GATTGCCAAA GCCTCAGGCT CCAACGTTCG CCTCAGATCC 
CAACTACTGC TGCAGAAATA TATGGCTCAG CGTAAATGGA AGAAACATTT CCATGTGGTG 
ACTGCAGTCA ACAGGCTAAG AAAATTTCCA ACGTGTCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255417.1
CDS	111..2072
Translation	MGTLCPVPALCRPSTRGQQRASDYAQIFTNKEQAEVAGVKPKHVLNTGSVQAATSRALWEESQKQDTPVGTVEGLPLIIDTSLKGADLTQAGASLRQGVEALDPGQEPPPTEAESRLPALASEDTGTTLELSVAIDRISEVLTSLRMSQSAGEGTSSSKPDCSEPGPQPLGPLTTDSDIHSDEGLPRISVRMREMTTPEELFETQGGSPIGSAEAPGPGTVLEDQIPKGARPFPPLPKRSCNNGGASAEEATGPGAEPIRGPSLVTRDWRDEPVGTTDLQQGRDPGAVSPEPGKDHAAQGPGRTEAGRRVSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDTLISTSYTVSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNIKDREDVKNEINIMNQLSHVNLIQLYDAFESKNSFTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQTGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLNHLIAKASGSNVRLRSQLLLQKYMAQRKWKKHFHVVTAVNRLRKFPTCP

Target ORF information:

RefSeq Version	XM_006255355.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin light chain kinase 3 (Mylk3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255355.2

ORF Insert Sequence:

ATGGGAACTC TGTGCCCTGT CCCAGCTCTG TGCAGGCCCA GCACCCGAGG CCAGCAGCGG 
GCATCAGATT ACGCCCAGAT ATTCACGAAC AAGGAGCAAG CAGAAGTGGC TGGAGTGAAG 
CCAAAGCATG TGCTGAATAC AGGAAGTGTG CAAGCTGCCA CTTCTAGGGC GCTGTGGGAA 
GAGAGCCAGA AGCAGGACAC ACCCGTGGGG ACAGTGGAGG GGCTGCCTCT CATCATCGAT 
ACGTCACTGA AGGGAGCTGA CCTCACCCAG GCTGGAGCCT CACTGAGGCA GGGAGTTGAA 
GCTCTTGACC CAGGCCAAGA ACCCCCACCC ACAGAGGCAG AATCCAGGCT TCCTGCACTA 
GCCAGCGAGG ACACTGGGAC CACCCTGGAA TTGTCTGTAG CAATTGACAG AATCAGTGAG 
GTCCTCACTA GCCTCAGGAT GTCCCAAAGT GCTGGCGAAG GAACCTCATC CAGCAAGCCT 
GACTGTTCAG AGCCTGGCCC TCAGCCCCTA GGGCCACTAA CTACAGACAG TGACATTCAC 
AGTGATGAAG GACTTCCCAG GATCTCTGTC CGTATGCGAG AGATGACTAC TCCTGAGGAG 
CTGTTTGAGA CCCAAGGTGG CAGCCCCATT GGCTCGGCAG AAGCTCCAGG CCCTGGAACT 
GTGTTAGAAG ACCAGATCCC TAAAGGAGCC AGACCATTTC CACCCCTGCC AAAGAGGAGC 
TGCAACAATG GTGGCGCGAG TGCAGAGGAG GCAACAGGGC CTGGGGCTGA GCCCATCAGA 
GGACCAAGCT TGGTCACAAG GGACTGGAGA GATGAACCTG TTGGGACCAC AGACCTGCAG 
CAAGGCAGAG ACCCAGGAGC GGTGAGCCCT GAACCTGGGA AGGACCATGC AGCCCAGGGC 
CCAGGGAGAA CCGAAGCTGG AAGGAGGGTG TCTTCTGCTG CAGAGGCTGC CATCGTAGTT 
CTAGATGACA GCGCAGCACC CCCAGCCCCT TTTGAACACC GGGTAGTGAG CATCAAAGAC 
ACCCTCATCT CGACAAGTTA CACAGTGTCC CAACACGAAG TCTTGGGAGG GGGCCGGTTT 
GGCCAGGTGC ACAGGTGTAC AGAGCGGTCC ACAGGCCTTG CACTGGCAGC CAAGATCATC 
AAAGTGAAGA ACATAAAGGA CCGGGAGGAT GTGAAGAATG AGATCAACAT CATGAACCAG 
CTCAGCCACG TAAACTTGAT CCAACTTTAT GATGCTTTTG AGAGCAAGAA CAGCTTCACC 
CTGATCATGG AGTATGTGGA TGGAGGTGAA CTCTTCGACC GGATCACGGA TGAGAAGTAC 
CACCTGACCG AGCTGGATGT GGTCTTGTTC ACAAGGCAGA TCTGTGAGGG TGTGCATTAC 
CTGCACCAGC ACTACATTCT GCACCTGGAC CTCAAGCCAG AGAACATACT GTGTGTCAGC 
CAGACTGGGC ATCAAATTAA GATTATTGAC TTTGGGCTGG CTAGAAGATA TAAACCTCGG 
GAGAAGCTAA AGGTTAACTT TGGTACTCCA GAGTTCCTGG CTCCAGAAGT TGTTAACTAT 
GAGTTTGTCT CATTCCCAAC AGACATGTGG AGTGTGGGAG TTATCACCTA CATGCTACTC 
AGTGGTTTGT CCCCATTTCT AGGGGAAACA GATGCAGAGA CCATGAATTT TATTGTGAAC 
TGCAGCTGGG ATTTCGATGC TGATACCTTC AAAGGGCTGT CAGAGGAAGC CAAGGACTTT 
GTTTCACGGT TGCTGGTCAA AGAGAAGAGT TGTAGGATGA GCGCCACACA GTGCCTGAAA 
CATGAGTGGT TAAATCACCT GATTGCCAAA GCCTCAGGCT CCAACGTTCG CCTCAGATCC 
CAACTACTGC TGCAGAAATA TATGGCTCAG CGTAAATGGA AGAAACATTT CCATGTGGTG 
ACTGCAGTCA ACAGGCTAAG AAAATTTCCA ACGTGTCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The shift in GH3 cell shape and cell motility is dependent on MLCK and ROCK.
Experimental cell research354(1)1-17(2017 05)
?vila-Rodr?guez D,Solano Agama C,Gonz?lez-Pozos S,Vicente M?ndez-M?ndez J,Ortiz Plata A,Arreola-Mendoza L,Mendoza-Garrido ME

CaMKII in addition to MLCK contributes to phosphorylation of regulatory light chain in cardiomyocytes.
Biochemical and biophysical research communications471(1)219-25(2016 Feb)
Eikemo H,Moltzau LR,Hussain RI,Nguyen CH,Qvigstad E,Levy FO,Skomedal T,Osnes JB

Degradation of cardiac myosin light chain kinase by matrix metalloproteinase-2 contributes to myocardial contractile dysfunction during ischemia/reperfusion.
Journal of molecular and cellular cardiology77102-12(2014 Dec)
Gao L,Zheng YJ,Gu SS,Tan JL,Paul C,Wang YG,Yang HT

Mechanism of IL-1beta-induced increase in intestinal epithelial tight junction permeability.
Journal of immunology (Baltimore, Md. : 1950)180(8)5653-61(2008 Apr)
Al-Sadi R,Ye D,Dokladny K,Ma TY

Identification of cardiac-specific myosin light chain kinase.
Circulation research102(5)571-80(2008 Mar)
Chan JY,Takeda M,Briggs LE,Graham ML,Lu JT,Horikoshi N,Weinberg EO,Aoki H,Sato N,Chien KR,Kasahara H

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Mylk3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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ORF Insert Sequence:

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