Ski cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ski
Entrez Gene ID	20481
Full Name	ski sarcoma viral oncogene homolog (avian)
Synonyms	2310012I02Rik,2610001A11Rik,AA062172,AA589460,BC004088
General protein information	Preferred Names ski sarcoma viral oncogene homolog (avian) Names ski oncogene C-ski Sloan-Kettering viral oncogene homolog proto-oncogene c-Ski
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 E2\|4 86.17 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001357191.1	NP_001344120.1	ski oncogene isoform 2
XM_006538685.3	XP_006538748.1	ski oncogene isoform X1
NM_011385.2	NP_035515.2	ski oncogene isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ski	NP_035515.2
Gallus gallus (chicken)	SKI	NP_001034407.1
	SKI	XP_001086434.1
Canis lupus familiaris (dog)	SKI	XP_005620652.1
Danio rerio (zebrafish)	skia	NP_571010.2
Xenopus tropicalis (tropical clawed frog)	ski	XP_004916211.1
Homo sapiens (human)	SKI	NP_003027.1
Bos taurus (cattle)	SKI	XP_002694226.2
Rattus norvegicus (Norway rat)	Ski	XP_006239683.1

Gene Ontology
Bibliography

Related articles in PubMed

Reversing SKI-SMAD4-mediated suppression is essential for T
Zhang S, Takaku M, Zou L, Gu AD, Chou WC, Zhang G, Wu B, Kong Q, Thomas SY, Serody JS, Chen X, Xu X, Wade PA, Cook DN, Ting JPY, Wan YY
Nature551(7678)105-109(2017 Nov)

Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate.
Wang W, Song B, Anbarchian T, Shirazyan A, Sadik JE, Lyons KM
PLoS genetics12(10)e1006352(2016 Oct)

Area-specific development of distinct projection neuron subclasses is regulated by postnatal epigenetic modifications.
Harb K, Magrinelli E, Nicolas CS, Lukianets N, Frangeul L, Pietri M, Sun T, Sandoz G, Grammont F, Jabaudon D, Studer M, Alfano C
eLife5e09531(2016 Jan)

A regulatory role for TGF-? signaling in the establishment and function of the thymic medulla.
Hauri-Hohl M, Zuklys S, Holl?nder GA, Ziegler SF
Nature immunology15(6)554-61(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our results demonstrate that SKI is an important regulator of hematopoietic stem cell activity and its overexpression leads to myeloproliferative disease.
Title: The SKI proto-oncogene enhances the in vivo repopulation of hematopoietic stem cells and causes myeloproliferative disease.

Trnsgenic mice overexpressing chicken Ski (c-Ski) have marked decrease in adipose mass with skeletal muscle hypertrophy Results suggest Ski plays a major role in skeletal muscle metabolism and adipogenesis and hence influences risk of obesity and diabetes
Title: Ski overexpression in skeletal muscle modulates genetic programs that control susceptibility to diet-induced obesity and insulin signaling.

Satb2 recruits Ski to the Ctip2 locus, and Ski attracts histone deacetylases, thereby enabling the formation of a functional nucleosome remodeling and deacetylase repressor complex.
Title: Protooncogene Ski cooperates with the chromatin-remodeling factor Satb2 in specifying callosal neurons.

The presented data support a new role for Ski in the mitotic process and in maintenance of genetic stability
Title: Chromosomal instability in mouse embryonic fibroblasts null for the transcriptional co-repressor Ski.

Data on expression of Ski suggest that Ski is involved in follicular development, oogenesis, ovulation, and luteinization. During the estrous cycle, Ski expression was apparent at proestrus and estrus, faint at metestrus, highest at diestrus.
Title: Relationship between Sloan-Kettering virus expression and mouse follicular development.

The following Ski gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ski cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08060	NM_011385.2 Latest version!	Mus musculus ski sarcoma viral oncogene homolog (avian) (Ski), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu34076	NM_001357191.1 Latest version!	Mus musculus ski sarcoma viral oncogene homolog (avian) (Ski), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu34076	XM_006538685.3 Latest version!	Mus musculus ski sarcoma viral oncogene homolog (avian) (Ski), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ski ( NM_011385.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035515.2 Mus musculus ski sarcoma viral oncogene homolog (avian) (Ski), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08060
Clone ID Related Accession (Same CDS sequence)	NM_011385.2
Accession Version	NM_011385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	ski oncogene isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL670413.19, CA750434.1, CA451127.1, CB722801.1, CN526656.1 and AW491584.1. On Aug 26, 2006 this sequence version replaced NM_011385.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.37676.1, AF435852.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCTG CAGCGGCCGG CCGGGGCGGC TTCCAGCCGC ACCCGGGGCT GCAGAAGACG 
CTGGAGCAGT TCCACCTGAG CTCCATGAGC TCGCTGGGCG GCCCGGCCGC TTTCTCGGCG 
CGCTGGGCGC AGGAGGCCTA CAAGAAGGAG AGCGCCAAGG AGGCGGGTGC GGCCACAGTG 
CCGGCGCCGG TGCCCACTGC CGCGGAGCCG CCGCCCGTGC TGCACCTGCC TGCCATCCAG 
CCGCCGCCGC CCGTGCTTCC CGGGCCCTTC TTCATGCCGT CGGACCGCTC CACCGAGCGC 
TGTGAGACCG TGCTGGAAGG GGAGACCATC TCTTGTTTCG TGGTGGGAGG TGAGAAGCGT 
CTGTGCCTGC CGCAGATCCT CAACTCGGTG CTGCGCGACT TCTCACTGCA GCAGATCAAC 
TCCGTGTGCG ATGAGCTGCA CATCTACTGC TCGCGCTGCA CCGCCGACCA GCTGGAGATC 
CTCAAAGTCA TGGGCATCCT GCCCTTCTCC GCGCCCTCCT GCGGGCTCAT CACTAAGACG 
GACGCCGAGC GCCTGTGCAA CGCGCTGCTC TACGGCGGCG CCTACCCGCC GCCTTGCAAG 
AAGGAGTTGG CGGCCAGCCT GGCGCTGGGC CTGGAGCTCA GCGAACGCAG CGTCCGCGTG 
TACCACGAGT GCTTCGGCAA GTGCAAAGGG CTGCTGGTGC CCGAGCTGTA CAGCAGTCCC 
AGCGCTGCCT GCATCCAGTG CCTCGACTGC CGCCTCATGT ACCCGCCGCA CAAGTTCGTT 
GTACACTCGC ACAAGGCCCT GGAGAACCGG ACCTGCCACT GGGGCTTCGA CTCAGCCAAC 
TGGCGGGCCT ACATCCTGCT GAGCCAGGAC TACACGGGCA AGGAGGAGCA GGCCCGCCTT 
GGCCGCTGCC TGGACGACGT GAAGGAGAAG TTCGACTATG CCAACAAGTA CAAGCGGAGG 
GTGCCCCGGG TCTCAGAGCC CCCAGCTTCC ATAAGACCCA AAACAGACGA CACTTCCTCA 
CAGTCCCCTG CGTCTTCTGA GAAGGACAAG CAGTCCACTT GGCTGCGGAC CTTGGCTGGT 
TCCTCCAATA AGAGCCTCGG CTGCACTCAC CCTCGCCAGC GCCTTTCTGC TTTCCGGCCC 
TGGTCCCCTG CAGTGTCTGC GAGTGAGAAA GAGACGTCCC CACACCTCCC AGCTTTGATT 
CGAGACAGCT TCTACTCCTA CAAGAGCTTT GAGACAGCCG TGGCCCCCAA TGTGGCCCTA 
GCACCACCAA CCCAGCAAAA GGTTGTGAAC AGTCCACCCT GCACCACCGT GGTCTCCCGG 
GCACCAGAGC CTCTTACTAC TTGTATCCAG CCACGAAAAC GGAAGCTGAC TCTGGACACA 
GCAGGAGCCC CAGACATGCT GACACCTGTG GCTGCTGCAG AGGAAGACAA GGATTCTGAG 
GCCGAGGTTG AAGTTGAAAG CAGAGAGGAG TTCACCTCCT CCTTGTCCTC GCTGTCCTCT 
CCGTCCTTTA CCTCGTCCAG CTCTGCCAAG GATCTAAGCT CCCCAGGCAT GCATGCTCCG 
CCCGTGGTTG CCCCCGATGC TGCAGCCCAT GTTGATGCCC CAAGTGGGCT GGAGGCAGAG 
TTGGAGCACC TGCGGCAAGC CCTGGAGGGT GGCCTGGATA CTAAGGAAGC CAAAGAAAAG 
TTCCTGCATG AGGTGGTCAA AATGCGAGTT AAGCAAGAGG AAAAGCTGAC AGCAGCCCTG 
CAGGCTAAGC GCAGCCTGCA CCAGGAGCTA GAATTCTTGC GTGTGGCCAA GAAGGAGAAA 
CTGCGGGAAG CCACAGAGGC CAAGCGCAGT TTGAGGAAAG AGATTGAGCG GCTCCGAGCT 
GAAAACGAGA AGAAGATGAA AGAGGCCAAC GAGTCCCGGG TGCGCCTGAA GCGAGAGCTG 
GAGCAGGCGC GGCAGGTCCG GGTGTGTGAC AAGGGCTGCG AGGCCGGGCG CCTGCGTGCC 
AAGTACTCAG CCCAGATTGA GGACCTGCAA GCGAAGCTGC AGCATGCGGA GGCCGACCGC 
GAGCAGCTTC GAGCTGACCT GCTGCGGGAA CGTGAGGCCC GCGAGCATCT GGAGAAGGTG 
GTGAGGGAGC TTCAGGAGCA GCTGCGGCCT CGGCCACGGC CTGAGCATCC TGGAGGTGAG 
AGCAACGCTG AGCTGGGTCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035515.2
CDS	16..2199
Translation	MEAAAAGRGGFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKESAKEAGAATVPAPVPTAAEPPPVLHLPAIQPPPPVLPGPFFMPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNSVLRDFSLQQINSVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYGGAYPPPCKKELAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVHSHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYANKYKRRVPRVSEPPASIRPKTDDTSSQSPASSEKDKQSTWLRTLAGSSNKSLGCTHPRQRLSAFRPWSPAVSASEKETSPHLPALIRDSFYSYKSFETAVAPNVALAPPTQQKVVNSPPCTTVVSRAPEPLTTCIQPRKRKLTLDTAGAPDMLTPVAAAEEDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLSSPGMHAPPVVAPDAAAHVDAPSGLEAELEHLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLTAALQAKRSLHQELEFLRVAKKEKLREATEAKRSLRKEIERLRAENEKKMKEANESRVRLKRELEQARQVRVCDKGCEAGRLRAKYSAQIEDLQAKLQHAEADREQLRADLLREREAREHLEKVVRELQEQLRPRPRPEHPGGESNAELGP

Target ORF information:

RefSeq Version	NM_011385.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ski sarcoma viral oncogene homolog (avian) (Ski), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011385.2

ORF Insert Sequence:

ATGGAAGCTG CAGCGGCCGG CCGGGGCGGC TTCCAGCCGC ACCCGGGGCT GCAGAAGACG 
CTGGAGCAGT TCCACCTGAG CTCCATGAGC TCGCTGGGCG GCCCGGCCGC TTTCTCGGCG 
CGCTGGGCGC AGGAGGCCTA CAAGAAGGAG AGCGCCAAGG AGGCGGGTGC GGCCACAGTG 
CCGGCGCCGG TGCCCACTGC CGCGGAGCCG CCGCCCGTGC TGCACCTGCC TGCCATCCAG 
CCGCCGCCGC CCGTGCTTCC CGGGCCCTTC TTCATGCCGT CGGACCGCTC CACCGAGCGC 
TGTGAGACCG TGCTGGAAGG GGAGACCATC TCTTGTTTCG TGGTGGGAGG TGAGAAGCGT 
CTGTGCCTGC CGCAGATCCT CAACTCGGTG CTGCGCGACT TCTCACTGCA GCAGATCAAC 
TCCGTGTGCG ATGAGCTGCA CATCTACTGC TCGCGCTGCA CCGCCGACCA GCTGGAGATC 
CTCAAAGTCA TGGGCATCCT GCCCTTCTCC GCGCCCTCCT GCGGGCTCAT CACTAAGACG 
GACGCCGAGC GCCTGTGCAA CGCGCTGCTC TACGGCGGCG CCTACCCGCC GCCTTGCAAG 
AAGGAGTTGG CGGCCAGCCT GGCGCTGGGC CTGGAGCTCA GCGAACGCAG CGTCCGCGTG 
TACCACGAGT GCTTCGGCAA GTGCAAAGGG CTGCTGGTGC CCGAGCTGTA CAGCAGTCCC 
AGCGCTGCCT GCATCCAGTG CCTCGACTGC CGCCTCATGT ACCCGCCGCA CAAGTTCGTT 
GTACACTCGC ACAAGGCCCT GGAGAACCGG ACCTGCCACT GGGGCTTCGA CTCAGCCAAC 
TGGCGGGCCT ACATCCTGCT GAGCCAGGAC TACACGGGCA AGGAGGAGCA GGCCCGCCTT 
GGCCGCTGCC TGGACGACGT GAAGGAGAAG TTCGACTATG CCAACAAGTA CAAGCGGAGG 
GTGCCCCGGG TCTCAGAGCC CCCAGCTTCC ATAAGACCCA AAACAGACGA CACTTCCTCA 
CAGTCCCCTG CGTCTTCTGA GAAGGACAAG CAGTCCACTT GGCTGCGGAC CTTGGCTGGT 
TCCTCCAATA AGAGCCTCGG CTGCACTCAC CCTCGCCAGC GCCTTTCTGC TTTCCGGCCC 
TGGTCCCCTG CAGTGTCTGC GAGTGAGAAA GAGACGTCCC CACACCTCCC AGCTTTGATT 
CGAGACAGCT TCTACTCCTA CAAGAGCTTT GAGACAGCCG TGGCCCCCAA TGTGGCCCTA 
GCACCACCAA CCCAGCAAAA GGTTGTGAAC AGTCCACCCT GCACCACCGT GGTCTCCCGG 
GCACCAGAGC CTCTTACTAC TTGTATCCAG CCACGAAAAC GGAAGCTGAC TCTGGACACA 
GCAGGAGCCC CAGACATGCT GACACCTGTG GCTGCTGCAG AGGAAGACAA GGATTCTGAG 
GCCGAGGTTG AAGTTGAAAG CAGAGAGGAG TTCACCTCCT CCTTGTCCTC GCTGTCCTCT 
CCGTCCTTTA CCTCGTCCAG CTCTGCCAAG GATCTAAGCT CCCCAGGCAT GCATGCTCCG 
CCCGTGGTTG CCCCCGATGC TGCAGCCCAT GTTGATGCCC CAAGTGGGCT GGAGGCAGAG 
TTGGAGCACC TGCGGCAAGC CCTGGAGGGT GGCCTGGATA CTAAGGAAGC CAAAGAAAAG 
TTCCTGCATG AGGTGGTCAA AATGCGAGTT AAGCAAGAGG AAAAGCTGAC AGCAGCCCTG 
CAGGCTAAGC GCAGCCTGCA CCAGGAGCTA GAATTCTTGC GTGTGGCCAA GAAGGAGAAA 
CTGCGGGAAG CCACAGAGGC CAAGCGCAGT TTGAGGAAAG AGATTGAGCG GCTCCGAGCT 
GAAAACGAGA AGAAGATGAA AGAGGCCAAC GAGTCCCGGG TGCGCCTGAA GCGAGAGCTG 
GAGCAGGCGC GGCAGGTCCG GGTGTGTGAC AAGGGCTGCG AGGCCGGGCG CCTGCGTGCC 
AAGTACTCAG CCCAGATTGA GGACCTGCAA GCGAAGCTGC AGCATGCGGA GGCCGACCGC 
GAGCAGCTTC GAGCTGACCT GCTGCGGGAA CGTGAGGCCC GCGAGCATCT GGAGAAGGTG 
GTGAGGGAGC TTCAGGAGCA GCTGCGGCCT CGGCCACGGC CTGAGCATCC TGGAGGTGAG 
AGCAACGCTG AGCTGGGTCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ski ( NM_001357191.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344120.1 Mus musculus ski sarcoma viral oncogene homolog (avian) (Ski), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu34076
Clone ID Related Accession (Same CDS sequence)	NM_001357191.1 , XM_006538685.3
Accession Version	NM_001357191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	ski oncogene isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL670413.19, CA750434.1, CB522577.1, CB722801.1, CN526656.1 and AW491584.1. On Oct 24, 2017 this sequence version replaced XM_006538685.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.196807.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCTG CAGCGGCCGG CCGGGGCGGC TTCCAGCCGC ACCCGGGGCT GCAGAAGACG 
CTGGAGCAGT TCCACCTGAG CTCCATGAGC TCGCTGGGCG GCCCGGCCGC TTTCTCGGCG 
CGCTGGGCGC AGGAGGCCTA CAAGAAGGAG AGCGCCAAGG AGGCGGGTGC GGCCACAGTG 
CCGGCGCCGG TGCCCACTGC CGCGGAGCCG CCGCCCGTGC TGCACCTGCC TGCCATCCAG 
CCGCCGCCGC CCGTGCTTCC CGGGCCCTTC TTCATGCCGT CGGACCGCTC CACCGAGCGC 
TGTGAGACCG TGCTGGAAGG GGAGACCATC TCTTGTTTCG TGGTGGGAGG TGAGAAGCGT 
CTGTGCCTGC CGCAGATCCT CAACTCGGTG CTGCGCGACT TCTCACTGCA GCAGATCAAC 
TCCGTGTGCG ATGAGCTGCA CATCTACTGC TCGCGCTGCA CCGCCGACCA GCTGGAGATC 
CTCAAAGTCA TGGGCATCCT GCCCTTCTCC GCGCCCTCCT GCGGGCTCAT CACTAAGACG 
GACGCCGAGC GCCTGTGCAA CGCGCTGCTC TACGGCGGCG CCTACCCGCC GCCTTGCAAG 
AAGGAGTTGG CGGCCAGCCT GGCGCTGGGC CTGGAGCTCA GCGAACGCAG CGTCCGCGTG 
TACCACGAGT GCTTCGGCAA GTGCAAAGGG CTGCTGGTGC CCGAGCTGTA CAGCAGTCCC 
AGCGCTGCCT GCATCCAGTG CCTCGACTGC CGCCTCATGT ACCCGCCGCA CAAGTTCGTT 
GTACACTCGC ACAAGGCCCT GGAGAACCGG ACCTGCCACT GGGGCTTCGA CTCAGCCAAC 
TGGCGGGCCT ACATCCTGCT GAGCCAGGAC TACACGGGCA AGGAGGAGCA GGCCCGCCTT 
GGCCGCTGCC TGGACGACGT GAAGGAGAAG TTCGACTATG CCAACAAGTA CAAGCGGAGG 
GTGCCCCGGA GCCTCGGCTG CACTCACCCT CGCCAGCGCC TTTCTGCTTT CCGGCCCTGG 
TCCCCTGCAG TGTCTGCGAG TGAGAAAGAG ACGTCCCCAC ACCTCCCAGC TTTGATTCGA 
GACAGCTTCT ACTCCTACAA GAGCTTTGAG ACAGCCGTGG CCCCCAATGT GGCCCTAGCA 
CCACCAACCC AGCAAAAGGT TGTGAACAGT CCACCCTGCA CCACCGTGGT CTCCCGGGCA 
CCAGAGCCTC TTACTACTTG TATCCAGCCA CGAAAACGGA AGCTGACTCT GGACACAGCA 
GGAGCCCCAG ACATGCTGAC ACCTGTGGCT GCTGCAGAGG AAGACAAGGA TTCTGAGGCC 
GAGGTTGAAG TTGAAAGCAG AGAGGAGTTC ACCTCCTCCT TGTCCTCGCT GTCCTCTCCG 
TCCTTTACCT CGTCCAGCTC TGCCAAGGAT CTAAGCTCCC CAGGCATGCA TGCTCCGCCC 
GTGGTTGCCC CCGATGCTGC AGCCCATGTT GATGCCCCAA GTGGGCTGGA GGCAGAGTTG 
GAGCACCTGC GGCAAGCCCT GGAGGGTGGC CTGGATACTA AGGAAGCCAA AGAAAAGTTC 
CTGCATGAGG TGGTCAAAAT GCGAGTTAAG CAAGAGGAAA AGCTGACAGC AGCCCTGCAG 
GCTAAGCGCA GCCTGCACCA GGAGCTAGAA TTCTTGCGTG TGGCCAAGAA GGAGAAACTG 
CGGGAAGCCA CAGAGGCCAA GCGCAGTTTG AGGAAAGAGA TTGAGCGGCT CCGAGCTGAA 
AACGAGAAGA AGATGAAAGA GGCCAACGAG TCCCGGGTGC GCCTGAAGCG AGAGCTGGAG 
CAGGCGCGGC AGGTCCGGGT GTGTGACAAG GGCTGCGAGG CCGGGCGCCT GCGTGCCAAG 
TACTCAGCCC AGATTGAGGA CCTGCAAGCG AAGCTGCAGC ATGCGGAGGC CGACCGCGAG 
CAGCTTCGAG CTGACCTGCT GCGGGAACGT GAGGCCCGCG AGCATCTGGA GAAGGTGGTG 
AGGGAGCTTC AGGAGCAGCT GCGGCCTCGG CCACGGCCTG AGCATCCTGG AGGTGAGAGC 
AACGCTGAGC TGGGTCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344120.1
CDS	16..2076
Translation	MEAAAAGRGGFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKESAKEAGAATVPAPVPTAAEPPPVLHLPAIQPPPPVLPGPFFMPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNSVLRDFSLQQINSVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYGGAYPPPCKKELAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVHSHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYANKYKRRVPRSLGCTHPRQRLSAFRPWSPAVSASEKETSPHLPALIRDSFYSYKSFETAVAPNVALAPPTQQKVVNSPPCTTVVSRAPEPLTTCIQPRKRKLTLDTAGAPDMLTPVAAAEEDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLSSPGMHAPPVVAPDAAAHVDAPSGLEAELEHLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLTAALQAKRSLHQELEFLRVAKKEKLREATEAKRSLRKEIERLRAENEKKMKEANESRVRLKRELEQARQVRVCDKGCEAGRLRAKYSAQIEDLQAKLQHAEADREQLRADLLREREAREHLEKVVRELQEQLRPRPRPEHPGGESNAELGP

Target ORF information:

RefSeq Version	NM_001357191.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ski sarcoma viral oncogene homolog (avian) (Ski), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357191.1

ORF Insert Sequence:

ATGGAAGCTG CAGCGGCCGG CCGGGGCGGC TTCCAGCCGC ACCCGGGGCT GCAGAAGACG 
CTGGAGCAGT TCCACCTGAG CTCCATGAGC TCGCTGGGCG GCCCGGCCGC TTTCTCGGCG 
CGCTGGGCGC AGGAGGCCTA CAAGAAGGAG AGCGCCAAGG AGGCGGGTGC GGCCACAGTG 
CCGGCGCCGG TGCCCACTGC CGCGGAGCCG CCGCCCGTGC TGCACCTGCC TGCCATCCAG 
CCGCCGCCGC CCGTGCTTCC CGGGCCCTTC TTCATGCCGT CGGACCGCTC CACCGAGCGC 
TGTGAGACCG TGCTGGAAGG GGAGACCATC TCTTGTTTCG TGGTGGGAGG TGAGAAGCGT 
CTGTGCCTGC CGCAGATCCT CAACTCGGTG CTGCGCGACT TCTCACTGCA GCAGATCAAC 
TCCGTGTGCG ATGAGCTGCA CATCTACTGC TCGCGCTGCA CCGCCGACCA GCTGGAGATC 
CTCAAAGTCA TGGGCATCCT GCCCTTCTCC GCGCCCTCCT GCGGGCTCAT CACTAAGACG 
GACGCCGAGC GCCTGTGCAA CGCGCTGCTC TACGGCGGCG CCTACCCGCC GCCTTGCAAG 
AAGGAGTTGG CGGCCAGCCT GGCGCTGGGC CTGGAGCTCA GCGAACGCAG CGTCCGCGTG 
TACCACGAGT GCTTCGGCAA GTGCAAAGGG CTGCTGGTGC CCGAGCTGTA CAGCAGTCCC 
AGCGCTGCCT GCATCCAGTG CCTCGACTGC CGCCTCATGT ACCCGCCGCA CAAGTTCGTT 
GTACACTCGC ACAAGGCCCT GGAGAACCGG ACCTGCCACT GGGGCTTCGA CTCAGCCAAC 
TGGCGGGCCT ACATCCTGCT GAGCCAGGAC TACACGGGCA AGGAGGAGCA GGCCCGCCTT 
GGCCGCTGCC TGGACGACGT GAAGGAGAAG TTCGACTATG CCAACAAGTA CAAGCGGAGG 
GTGCCCCGGA GCCTCGGCTG CACTCACCCT CGCCAGCGCC TTTCTGCTTT CCGGCCCTGG 
TCCCCTGCAG TGTCTGCGAG TGAGAAAGAG ACGTCCCCAC ACCTCCCAGC TTTGATTCGA 
GACAGCTTCT ACTCCTACAA GAGCTTTGAG ACAGCCGTGG CCCCCAATGT GGCCCTAGCA 
CCACCAACCC AGCAAAAGGT TGTGAACAGT CCACCCTGCA CCACCGTGGT CTCCCGGGCA 
CCAGAGCCTC TTACTACTTG TATCCAGCCA CGAAAACGGA AGCTGACTCT GGACACAGCA 
GGAGCCCCAG ACATGCTGAC ACCTGTGGCT GCTGCAGAGG AAGACAAGGA TTCTGAGGCC 
GAGGTTGAAG TTGAAAGCAG AGAGGAGTTC ACCTCCTCCT TGTCCTCGCT GTCCTCTCCG 
TCCTTTACCT CGTCCAGCTC TGCCAAGGAT CTAAGCTCCC CAGGCATGCA TGCTCCGCCC 
GTGGTTGCCC CCGATGCTGC AGCCCATGTT GATGCCCCAA GTGGGCTGGA GGCAGAGTTG 
GAGCACCTGC GGCAAGCCCT GGAGGGTGGC CTGGATACTA AGGAAGCCAA AGAAAAGTTC 
CTGCATGAGG TGGTCAAAAT GCGAGTTAAG CAAGAGGAAA AGCTGACAGC AGCCCTGCAG 
GCTAAGCGCA GCCTGCACCA GGAGCTAGAA TTCTTGCGTG TGGCCAAGAA GGAGAAACTG 
CGGGAAGCCA CAGAGGCCAA GCGCAGTTTG AGGAAAGAGA TTGAGCGGCT CCGAGCTGAA 
AACGAGAAGA AGATGAAAGA GGCCAACGAG TCCCGGGTGC GCCTGAAGCG AGAGCTGGAG 
CAGGCGCGGC AGGTCCGGGT GTGTGACAAG GGCTGCGAGG CCGGGCGCCT GCGTGCCAAG 
TACTCAGCCC AGATTGAGGA CCTGCAAGCG AAGCTGCAGC ATGCGGAGGC CGACCGCGAG 
CAGCTTCGAG CTGACCTGCT GCGGGAACGT GAGGCCCGCG AGCATCTGGA GAAGGTGGTG 
AGGGAGCTTC AGGAGCAGCT GCGGCCTCGG CCACGGCCTG AGCATCCTGG AGGTGAGAGC 
AACGCTGAGC TGGGTCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ski ( XM_006538685.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538748.1 Mus musculus ski sarcoma viral oncogene homolog (avian) (Ski), transcript variant X1, mRNA.

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CloneID	OMu34076
Clone ID Related Accession (Same CDS sequence)	NM_001357191.1 , XM_006538685.3
Accession Version	XM_006538685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	ski oncogene isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538685.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTG CAGCGGCCGG CCGGGGCGGC TTCCAGCCGC ACCCGGGGCT GCAGAAGACG 
CTGGAGCAGT TCCACCTGAG CTCCATGAGC TCGCTGGGCG GCCCGGCCGC TTTCTCGGCG 
CGCTGGGCGC AGGAGGCCTA CAAGAAGGAG AGCGCCAAGG AGGCGGGTGC GGCCACAGTG 
CCGGCGCCGG TGCCCACTGC CGCGGAGCCG CCGCCCGTGC TGCACCTGCC TGCCATCCAG 
CCGCCGCCGC CCGTGCTTCC CGGGCCCTTC TTCATGCCGT CGGACCGCTC CACCGAGCGC 
TGTGAGACCG TGCTGGAAGG GGAGACCATC TCTTGTTTCG TGGTGGGAGG TGAGAAGCGT 
CTGTGCCTGC CGCAGATCCT CAACTCGGTG CTGCGCGACT TCTCACTGCA GCAGATCAAC 
TCCGTGTGCG ATGAGCTGCA CATCTACTGC TCGCGCTGCA CCGCCGACCA GCTGGAGATC 
CTCAAAGTCA TGGGCATCCT GCCCTTCTCC GCGCCCTCCT GCGGGCTCAT CACTAAGACG 
GACGCCGAGC GCCTGTGCAA CGCGCTGCTC TACGGCGGCG CCTACCCGCC GCCTTGCAAG 
AAGGAGTTGG CGGCCAGCCT GGCGCTGGGC CTGGAGCTCA GCGAACGCAG CGTCCGCGTG 
TACCACGAGT GCTTCGGCAA GTGCAAAGGG CTGCTGGTGC CCGAGCTGTA CAGCAGTCCC 
AGCGCTGCCT GCATCCAGTG CCTCGACTGC CGCCTCATGT ACCCGCCGCA CAAGTTCGTT 
GTACACTCGC ACAAGGCCCT GGAGAACCGG ACCTGCCACT GGGGCTTCGA CTCAGCCAAC 
TGGCGGGCCT ACATCCTGCT GAGCCAGGAC TACACGGGCA AGGAGGAGCA GGCCCGCCTT 
GGCCGCTGCC TGGACGACGT GAAGGAGAAG TTCGACTATG CCAACAAGTA CAAGCGGAGG 
GTGCCCCGGA GCCTCGGCTG CACTCACCCT CGCCAGCGCC TTTCTGCTTT CCGGCCCTGG 
TCCCCTGCAG TGTCTGCGAG TGAGAAAGAG ACGTCCCCAC ACCTCCCAGC TTTGATTCGA 
GACAGCTTCT ACTCCTACAA GAGCTTTGAG ACAGCCGTGG CCCCCAATGT GGCCCTAGCA 
CCACCAACCC AGCAAAAGGT TGTGAACAGT CCACCCTGCA CCACCGTGGT CTCCCGGGCA 
CCAGAGCCTC TTACTACTTG TATCCAGCCA CGAAAACGGA AGCTGACTCT GGACACAGCA 
GGAGCCCCAG ACATGCTGAC ACCTGTGGCT GCTGCAGAGG AAGACAAGGA TTCTGAGGCC 
GAGGTTGAAG TTGAAAGCAG AGAGGAGTTC ACCTCCTCCT TGTCCTCGCT GTCCTCTCCG 
TCCTTTACCT CGTCCAGCTC TGCCAAGGAT CTAAGCTCCC CAGGCATGCA TGCTCCGCCC 
GTGGTTGCCC CCGATGCTGC AGCCCATGTT GATGCCCCAA GTGGGCTGGA GGCAGAGTTG 
GAGCACCTGC GGCAAGCCCT GGAGGGTGGC CTGGATACTA AGGAAGCCAA AGAAAAGTTC 
CTGCATGAGG TGGTCAAAAT GCGAGTTAAG CAAGAGGAAA AGCTGACAGC AGCCCTGCAG 
GCTAAGCGCA GCCTGCACCA GGAGCTAGAA TTCTTGCGTG TGGCCAAGAA GGAGAAACTG 
CGGGAAGCCA CAGAGGCCAA GCGCAGTTTG AGGAAAGAGA TTGAGCGGCT CCGAGCTGAA 
AACGAGAAGA AGATGAAAGA GGCCAACGAG TCCCGGGTGC GCCTGAAGCG AGAGCTGGAG 
CAGGCGCGGC AGGTCCGGGT GTGTGACAAG GGCTGCGAGG CCGGGCGCCT GCGTGCCAAG 
TACTCAGCCC AGATTGAGGA CCTGCAAGCG AAGCTGCAGC ATGCGGAGGC CGACCGCGAG 
CAGCTTCGAG CTGACCTGCT GCGGGAACGT GAGGCCCGCG AGCATCTGGA GAAGGTGGTG 
AGGGAGCTTC AGGAGCAGCT GCGGCCTCGG CCACGGCCTG AGCATCCTGG AGGTGAGAGC 
AACGCTGAGC TGGGTCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538748.1
CDS	73..2133
Translation	MEAAAAGRGGFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKESAKEAGAATVPAPVPTAAEPPPVLHLPAIQPPPPVLPGPFFMPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNSVLRDFSLQQINSVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYGGAYPPPCKKELAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVHSHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYANKYKRRVPRSLGCTHPRQRLSAFRPWSPAVSASEKETSPHLPALIRDSFYSYKSFETAVAPNVALAPPTQQKVVNSPPCTTVVSRAPEPLTTCIQPRKRKLTLDTAGAPDMLTPVAAAEEDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLSSPGMHAPPVVAPDAAAHVDAPSGLEAELEHLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLTAALQAKRSLHQELEFLRVAKKEKLREATEAKRSLRKEIERLRAENEKKMKEANESRVRLKRELEQARQVRVCDKGCEAGRLRAKYSAQIEDLQAKLQHAEADREQLRADLLREREAREHLEKVVRELQEQLRPRPRPEHPGGESNAELGP

Target ORF information:

RefSeq Version	XM_006538685.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus ski sarcoma viral oncogene homolog (avian) (Ski), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538685.3

ORF Insert Sequence:

ATGGAAGCTG CAGCGGCCGG CCGGGGCGGC TTCCAGCCGC ACCCGGGGCT GCAGAAGACG 
CTGGAGCAGT TCCACCTGAG CTCCATGAGC TCGCTGGGCG GCCCGGCCGC TTTCTCGGCG 
CGCTGGGCGC AGGAGGCCTA CAAGAAGGAG AGCGCCAAGG AGGCGGGTGC GGCCACAGTG 
CCGGCGCCGG TGCCCACTGC CGCGGAGCCG CCGCCCGTGC TGCACCTGCC TGCCATCCAG 
CCGCCGCCGC CCGTGCTTCC CGGGCCCTTC TTCATGCCGT CGGACCGCTC CACCGAGCGC 
TGTGAGACCG TGCTGGAAGG GGAGACCATC TCTTGTTTCG TGGTGGGAGG TGAGAAGCGT 
CTGTGCCTGC CGCAGATCCT CAACTCGGTG CTGCGCGACT TCTCACTGCA GCAGATCAAC 
TCCGTGTGCG ATGAGCTGCA CATCTACTGC TCGCGCTGCA CCGCCGACCA GCTGGAGATC 
CTCAAAGTCA TGGGCATCCT GCCCTTCTCC GCGCCCTCCT GCGGGCTCAT CACTAAGACG 
GACGCCGAGC GCCTGTGCAA CGCGCTGCTC TACGGCGGCG CCTACCCGCC GCCTTGCAAG 
AAGGAGTTGG CGGCCAGCCT GGCGCTGGGC CTGGAGCTCA GCGAACGCAG CGTCCGCGTG 
TACCACGAGT GCTTCGGCAA GTGCAAAGGG CTGCTGGTGC CCGAGCTGTA CAGCAGTCCC 
AGCGCTGCCT GCATCCAGTG CCTCGACTGC CGCCTCATGT ACCCGCCGCA CAAGTTCGTT 
GTACACTCGC ACAAGGCCCT GGAGAACCGG ACCTGCCACT GGGGCTTCGA CTCAGCCAAC 
TGGCGGGCCT ACATCCTGCT GAGCCAGGAC TACACGGGCA AGGAGGAGCA GGCCCGCCTT 
GGCCGCTGCC TGGACGACGT GAAGGAGAAG TTCGACTATG CCAACAAGTA CAAGCGGAGG 
GTGCCCCGGA GCCTCGGCTG CACTCACCCT CGCCAGCGCC TTTCTGCTTT CCGGCCCTGG 
TCCCCTGCAG TGTCTGCGAG TGAGAAAGAG ACGTCCCCAC ACCTCCCAGC TTTGATTCGA 
GACAGCTTCT ACTCCTACAA GAGCTTTGAG ACAGCCGTGG CCCCCAATGT GGCCCTAGCA 
CCACCAACCC AGCAAAAGGT TGTGAACAGT CCACCCTGCA CCACCGTGGT CTCCCGGGCA 
CCAGAGCCTC TTACTACTTG TATCCAGCCA CGAAAACGGA AGCTGACTCT GGACACAGCA 
GGAGCCCCAG ACATGCTGAC ACCTGTGGCT GCTGCAGAGG AAGACAAGGA TTCTGAGGCC 
GAGGTTGAAG TTGAAAGCAG AGAGGAGTTC ACCTCCTCCT TGTCCTCGCT GTCCTCTCCG 
TCCTTTACCT CGTCCAGCTC TGCCAAGGAT CTAAGCTCCC CAGGCATGCA TGCTCCGCCC 
GTGGTTGCCC CCGATGCTGC AGCCCATGTT GATGCCCCAA GTGGGCTGGA GGCAGAGTTG 
GAGCACCTGC GGCAAGCCCT GGAGGGTGGC CTGGATACTA AGGAAGCCAA AGAAAAGTTC 
CTGCATGAGG TGGTCAAAAT GCGAGTTAAG CAAGAGGAAA AGCTGACAGC AGCCCTGCAG 
GCTAAGCGCA GCCTGCACCA GGAGCTAGAA TTCTTGCGTG TGGCCAAGAA GGAGAAACTG 
CGGGAAGCCA CAGAGGCCAA GCGCAGTTTG AGGAAAGAGA TTGAGCGGCT CCGAGCTGAA 
AACGAGAAGA AGATGAAAGA GGCCAACGAG TCCCGGGTGC GCCTGAAGCG AGAGCTGGAG 
CAGGCGCGGC AGGTCCGGGT GTGTGACAAG GGCTGCGAGG CCGGGCGCCT GCGTGCCAAG 
TACTCAGCCC AGATTGAGGA CCTGCAAGCG AAGCTGCAGC ATGCGGAGGC CGACCGCGAG 
CAGCTTCGAG CTGACCTGCT GCGGGAACGT GAGGCCCGCG AGCATCTGGA GAAGGTGGTG 
AGGGAGCTTC AGGAGCAGCT GCGGCCTCGG CCACGGCCTG AGCATCCTGG AGGTGAGAGC 
AACGCTGAGC TGGGTCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reversing SKI-SMAD4-mediated suppression is essential for T
Nature551(7678)105-109(2017 Nov)
Zhang S,Takaku M,Zou L,Gu AD,Chou WC,Zhang G,Wu B,Kong Q,Thomas SY,Serody JS,Chen X,Xu X,Wade PA,Cook DN,Ting JPY,Wan YY

Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate.
PLoS genetics12(10)e1006352(2016 Oct)
Wang W,Song B,Anbarchian T,Shirazyan A,Sadik JE,Lyons KM

Area-specific development of distinct projection neuron subclasses is regulated by postnatal epigenetic modifications.
eLife5e09531(2016 Jan)
Harb K,Magrinelli E,Nicolas CS,Lukianets N,Frangeul L,Pietri M,Sun T,Sandoz G,Grammont F,Jabaudon D,Studer M,Alfano C

A regulatory role for TGF-? signaling in the establishment and function of the thymic medulla.
Nature immunology15(6)554-61(2014 Jun)
Hauri-Hohl M,Zuklys S,Holl?nder GA,Ziegler SF

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Ski cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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