skia cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	skia
Entrez Gene ID	30101
Full Name	v-ski avian sarcoma viral oncogene homolog a
Synonyms	id:ibd1250
General protein information	Preferred Names v-ski avian sarcoma viral oncogene homolog a Names ski oncogene nuclear oncoprotein skia
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130935.2	NP_571010.2	ski oncogene
NM_130935.3	NP_571010.3	ski oncogene

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ski	NP_035515.2
Gallus gallus (chicken)	SKI	NP_001034407.1
	SKI	XP_001086434.1
Canis lupus familiaris (dog)	SKI	XP_005620652.1
Danio rerio (zebrafish)	skia	NP_571010.2
Xenopus tropicalis (tropical clawed frog)	ski	XP_004916211.1
Homo sapiens (human)	SKI	NP_003027.1
Bos taurus (cattle)	SKI	XP_002694226.2
Rattus norvegicus (Norway rat)	Ski	XP_006239683.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptome comparison reveals a genetic network regulating the lower temperature limit in fish.
Hu P, Liu M, Liu Y, Wang J, Zhang D, Niu H, Jiang S, Wang J, Zhang D, Han B, Xu Q, Chen L
Scientific reports628952(2016 Jun)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Fine mapping of the 1p36 deletion syndrome identifies mutation of PRDM16 as a cause of cardiomyopathy.
Arndt AK, Schafer S, Drenckhahn JD, Sabeh MK, Plovie ER, Caliebe A, Klopocki E, Musso G, Werdich AA, Kalwa H, Heinig M, Padera RF, Wassilew K, Bluhm J, Harnack C, Martitz J, Barton PJ, Greutmann M, Berger F, Hubner N, Siebert R, Kramer HH, Cook SA, MacRae CA, Klaassen S
American journal of human genetics93(1)67-77(2013 Jul)

Mutations in the TGF-? repressor SKI cause Shprintzen-Goldberg syndrome with aortic aneurysm.
Doyle AJ, Doyle JJ, Bessling SL, Maragh S, Lindsay ME, Schepers D, Gillis E, Mortier G, Homfray T, Sauls K, Norris RA, Huso ND, Leahy D, Mohr DW, Caulfield MJ, Scott AF, Destr?e A, Hennekam RC, Arn PH, Curry CJ, Van Laer L, McCallion AS, Loeys BL, Dietz HC
Nature genetics44(11)1249-54(2012 Nov)

A fish-specific transposable element shapes the repertoire of p53 target genes in zebrafish.
Micale L, Loviglio MN, Manzoni M, Fusco C, Augello B, Migliavacca E, Cotugno G, Monti E, Borsani G, Reymond A, Merla G
PloS one7(10)e46642(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following skia gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the skia cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02554	NM_130935.2 Latest version!	Danio rerio v-ski avian sarcoma viral oncogene homolog a (skia), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa159835	NM_130935.3 Latest version!	Danio rerio v-ski avian sarcoma viral oncogene homolog a (skia), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

skia ( NM_130935.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571010.2 Danio rerio v-ski avian sarcoma viral oncogene homolog a (skia), mRNA.

Price & Availability↑

CloneID	ODa02554
Clone ID Related Accession (Same CDS sequence)	NM_130935.2
Accession Version	NM_130935.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Danio rerio(zebrafish)
Product	ski oncogene
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC117641.1. On Jun 18, 2006 this sequence version replaced NM_130935.1. ##Evidence-Data-START## Transcript exon combination :: BC117641.1, AF060117.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACAG TAGCGCGACA GAGTTTCCAG CCTCACCCGG GACTGCAACA AACTCTCAAA 
CAGTTTCACC TGAGCTCCAT GATCTCCCTC GGAGGACCGG CCGCGTTCTC GGCCAGGTGG 
CAACACGATT TGCTCTTCAA GAAGGACGGT AAAGACATCC CGGAGCCCGT GCTGCACCTC 
CCCCCGATGC AGCCTCCTCC GGTGATGCCC GGGCCGCTTT TCATCCCGTC CGACCGCTCA 
ACCGAGAGGT GCGAGACGGT CCTGGAGGGT GAGACCATCT CGTGCTTCGT GGTGGGCGGC 
GAGAAGCGCC TGTGCCTGCC GCAGATCCTC AACACGGTGC TGCGGGATTT CTCGCTCCAG 
CAGATCAACT CTGTGTGCGA CGACCTGCAC ATCTACTGCT CGCGCTGCAC CGCCGACCAG 
CTGGAGATCC TGAAGGTGAT GGGCATCCTG CCGTTTGCCG CGCCGTCCTG CGGGCTCATC 
ACCAAGACGG ACGCGGAGCG GCTATGCAAC GCGCTCATCT ATGGGGGCAC GTACCCGCCG 
CACTCCAAGA AGGAGCTGTA CGGCTCTATT GAGCTGGAAC TCACCGAGAA GAGCTTCAAG 
ATCTACCACG AGTGCTTCGG GAAGTGTAAG GGTCTGCTGG TCCCGGAGCT CTACACAAGT 
CCCAACGCGC CCTGCATCCA ATGCATGGAC TGCAGGCTCA TGTACCCGAC CCATAAATTC 
GTGGTGCACA GTCACAAATC GCTGGAGAAC AGGACTTGCC ATTGGGGCTT TGACTCGGCC 
AACTGGAGGG CGTATGTGCT CCTGAGTCCG GATTACACGG GGAAAGAGGA GAAAGCGCGT 
CTGGAGCAGC TCCTGGATGA CATTAAGGAG AAATTTGATT TCTCCAATAA ATACAAGCGG 
AAAGCATCAC AGGCGTCTGA TCCCATTCCT GCCAAGAAGC CCAAACACGA TGACTTTCCA 
GCACAGTCAC CCTCAGGAGA CAAAGACAAG CAACCTGATT GGCTACGGTC CCTCTCAGCG 
TCAGCCAATA AGGGGCTCAA CTGCATTCAA CCCAGACAGA GACCGTCAGC CTTCAGGCCT 
TGGTCTCCAA ACATCTCTGT TAGCGATAAA GACGGAGCAG GCAGACCCGC TAGCATGTTA 
CGAGAAAGTT TCTACAACTA CAAGAGTCTG GAAAGTGCCG TGGCCCCAAA CGTGGCTCTA 
ACACCGCCGC AGAAAGGAGG ACAGGCCTCT CCGTCGGCCC CCAGCAGCTC ACATCCACAT 
GGGGCAGAGA GTGAAGGTGC CAGTCGCGGG AGGAAGAGGA GGCTGACGGG CGACACTCAA 
CCCAGTCCAC AGCCCTGCGC TGCGGCCACC GCCAGCAAAC CCCTGCCAGC CTCGCCAGAG 
GAGCACGAGT CAGATGTGGA GGTGGAGGTG GAGAGTCGAG AGGAATTAAC GTCATCGCTT 
TCCTCGCTCT CCTCCCCATC TTTTCCCTCC TCAAGCTCAG CTAAGGACTT GAGCTCTCCC 
TCAGGCCCTG TGGCCACTTT ACCAGCAGTA GTGACACCCG CCGCCGGACC CCCGGAGGGC 
CCCCTGCTGT CTGGGGGAGA GGGACACGCC ATGAATAACC TGGAGTCAGA GCTGGAGACG 
CTGAGACAGG CGCTGGACAA CGGCCTGGAC TCAAAAGAAT CCAAGGAAAA GTTTCTGCAT 
GAGATCGTGA AGATGAGAGT GAAGCAGGAG GAGAAGCTCG GATCCGCATT ACAGGCCAAA 
CGGAGCCTCC AGCAGGAGCT GGAGTTCCTC CGTGTTGCGA AGAAGGAGAA GCTCCGTGAG 
GCGACAGAAG CGAAAAGGAA CCTGCGCAAG GAGATCGAGC GCTTGCGTGC CGAAAGCGAG 
AGGAAGATGA GAGAGGCCAA CGAGTCCCGT ATGCGGCTGA AGAGAGAGCT GGAGCAGGCC 
AGACAGCTGC GGGTCTGTGA CAAGGGCTGC GAGGCCGGAC GCCTGAGGGC CAAATACTCT 
GCACAGATTG AAGACCTGCA AGTAAAGTTG CAGCACGCCG AGGCAGACCG AGAGCAGCTG 
AGAGCCGATC TGATCCATGA ACGGGAGGCG CGGGAGCACC TGGAAAGAGT GGTCAAGGAG 
CTACAGGTAC AGCTGTGGCC CAAAAGCCCC AGCAAGAATG GCACAGACGT GGCACACCCA 
CCTGCCAAAG ACTCCAACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571010.2
CDS	381..2564
Translation	METVARQSFQPHPGLQQTLKQFHLSSMISLGGPAAFSARWQHDLLFKKDGKDIPEPVLHLPPMQPPPVMPGPLFIPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNTVLRDFSLQQINSVCDDLHIYCSRCTADQLEILKVMGILPFAAPSCGLITKTDAERLCNALIYGGTYPPHSKKELYGSIELELTEKSFKIYHECFGKCKGLLVPELYTSPNAPCIQCMDCRLMYPTHKFVVHSHKSLENRTCHWGFDSANWRAYVLLSPDYTGKEEKARLEQLLDDIKEKFDFSNKYKRKASQASDPIPAKKPKHDDFPAQSPSGDKDKQPDWLRSLSASANKGLNCIQPRQRPSAFRPWSPNISVSDKDGAGRPASMLRESFYNYKSLESAVAPNVALTPPQKGGQASPSAPSSSHPHGAESEGASRGRKRRLTGDTQPSPQPCAAATASKPLPASPEEHESDVEVEVESREELTSSLSSLSSPSFPSSSSAKDLSSPSGPVATLPAVVTPAAGPPEGPLLSGGEGHAMNNLESELETLRQALDNGLDSKESKEKFLHEIVKMRVKQEEKLGSALQAKRSLQQELEFLRVAKKEKLREATEAKRNLRKEIERLRAESERKMREANESRMRLKRELEQARQLRVCDKGCEAGRLRAKYSAQIEDLQVKLQHAEADREQLRADLIHEREAREHLERVVKELQVQLWPKSPSKNGTDVAHPPAKDSNN

Target ORF information:

RefSeq Version	NM_130935.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio v-ski avian sarcoma viral oncogene homolog a (skia), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130935.2

ORF Insert Sequence:

ATGGAAACAG TAGCGCGACA GAGTTTCCAG CCTCACCCGG GACTGCAACA AACTCTCAAA 
CAGTTTCACC TGAGCTCCAT GATCTCCCTC GGAGGACCGG CCGCGTTCTC GGCCAGGTGG 
CAACACGATT TGCTCTTCAA GAAGGACGGT AAAGACATCC CGGAGCCCGT GCTGCACCTC 
CCCCCGATGC AGCCTCCTCC GGTGATGCCC GGGCCGCTTT TCATCCCGTC CGACCGCTCA 
ACCGAGAGGT GCGAGACGGT CCTGGAGGGT GAGACCATCT CGTGCTTCGT GGTGGGCGGC 
GAGAAGCGCC TGTGCCTGCC GCAGATCCTC AACACGGTGC TGCGGGATTT CTCGCTCCAG 
CAGATCAACT CTGTGTGCGA CGACCTGCAC ATCTACTGCT CGCGCTGCAC CGCCGACCAG 
CTGGAGATCC TGAAGGTGAT GGGCATCCTG CCGTTTGCCG CGCCGTCCTG CGGGCTCATC 
ACCAAGACGG ACGCGGAGCG GCTATGCAAC GCGCTCATCT ATGGGGGCAC GTACCCGCCG 
CACTCCAAGA AGGAGCTGTA CGGCTCTATT GAGCTGGAAC TCACCGAGAA GAGCTTCAAG 
ATCTACCACG AGTGCTTCGG GAAGTGTAAG GGTCTGCTGG TCCCGGAGCT CTACACAAGT 
CCCAACGCGC CCTGCATCCA ATGCATGGAC TGCAGGCTCA TGTACCCGAC CCATAAATTC 
GTGGTGCACA GTCACAAATC GCTGGAGAAC AGGACTTGCC ATTGGGGCTT TGACTCGGCC 
AACTGGAGGG CGTATGTGCT CCTGAGTCCG GATTACACGG GGAAAGAGGA GAAAGCGCGT 
CTGGAGCAGC TCCTGGATGA CATTAAGGAG AAATTTGATT TCTCCAATAA ATACAAGCGG 
AAAGCATCAC AGGCGTCTGA TCCCATTCCT GCCAAGAAGC CCAAACACGA TGACTTTCCA 
GCACAGTCAC CCTCAGGAGA CAAAGACAAG CAACCTGATT GGCTACGGTC CCTCTCAGCG 
TCAGCCAATA AGGGGCTCAA CTGCATTCAA CCCAGACAGA GACCGTCAGC CTTCAGGCCT 
TGGTCTCCAA ACATCTCTGT TAGCGATAAA GACGGAGCAG GCAGACCCGC TAGCATGTTA 
CGAGAAAGTT TCTACAACTA CAAGAGTCTG GAAAGTGCCG TGGCCCCAAA CGTGGCTCTA 
ACACCGCCGC AGAAAGGAGG ACAGGCCTCT CCGTCGGCCC CCAGCAGCTC ACATCCACAT 
GGGGCAGAGA GTGAAGGTGC CAGTCGCGGG AGGAAGAGGA GGCTGACGGG CGACACTCAA 
CCCAGTCCAC AGCCCTGCGC TGCGGCCACC GCCAGCAAAC CCCTGCCAGC CTCGCCAGAG 
GAGCACGAGT CAGATGTGGA GGTGGAGGTG GAGAGTCGAG AGGAATTAAC GTCATCGCTT 
TCCTCGCTCT CCTCCCCATC TTTTCCCTCC TCAAGCTCAG CTAAGGACTT GAGCTCTCCC 
TCAGGCCCTG TGGCCACTTT ACCAGCAGTA GTGACACCCG CCGCCGGACC CCCGGAGGGC 
CCCCTGCTGT CTGGGGGAGA GGGACACGCC ATGAATAACC TGGAGTCAGA GCTGGAGACG 
CTGAGACAGG CGCTGGACAA CGGCCTGGAC TCAAAAGAAT CCAAGGAAAA GTTTCTGCAT 
GAGATCGTGA AGATGAGAGT GAAGCAGGAG GAGAAGCTCG GATCCGCATT ACAGGCCAAA 
CGGAGCCTCC AGCAGGAGCT GGAGTTCCTC CGTGTTGCGA AGAAGGAGAA GCTCCGTGAG 
GCGACAGAAG CGAAAAGGAA CCTGCGCAAG GAGATCGAGC GCTTGCGTGC CGAAAGCGAG 
AGGAAGATGA GAGAGGCCAA CGAGTCCCGT ATGCGGCTGA AGAGAGAGCT GGAGCAGGCC 
AGACAGCTGC GGGTCTGTGA CAAGGGCTGC GAGGCCGGAC GCCTGAGGGC CAAATACTCT 
GCACAGATTG AAGACCTGCA AGTAAAGTTG CAGCACGCCG AGGCAGACCG AGAGCAGCTG 
AGAGCCGATC TGATCCATGA ACGGGAGGCG CGGGAGCACC TGGAAAGAGT GGTCAAGGAG 
CTACAGGTAC AGCTGTGGCC CAAAAGCCCC AGCAAGAATG GCACAGACGT GGCACACCCA 
CCTGCCAAAG ACTCCAACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

skia ( NM_130935.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571010.3 Danio rerio v-ski avian sarcoma viral oncogene homolog a (skia), mRNA.

Price & Availability↑

CloneID	ODa159835
Clone ID Related Accession (Same CDS sequence)	NM_130935.3
Accession Version	NM_130935.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	ski oncogene
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU571083.4, CT573407.5 and BX664713.6. On Apr 11, 2018 this sequence version replaced NM_130935.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC117641.1, GFIL01038134.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAACAG TAGCGCGACA GAGTTTCCAG CCTCACCCGG GACTGCAACA AACTCTCAAA 
CAGTTTCACC TGAGCTCCAT GATCTCCCTC GGAGGACCGG CCGCGTTCTC GGCCAGGTGG 
CAACACGATT TGCTCTTCAA GAAGGACGGT AAAGACATCC CGGAGCCCGT GCTGCACCTC 
CCCCCGATGC AGCCTCCTCC GGTGATGCCC GGGCCGCTCT TCATCCCGTC CGACCGCTCA 
ACCGAGAGGT GCGAGACGGT CCTGGAGGGT GAGACCATCT CGTGCTTCGT GGTGGGCGGC 
GAGAAGCGCC TGTGCCTGCC GCAGATCCTC AACACGGTGC TGCGGGATTT CTCGCTCCAG 
CAGATCAACT CTGTGTGCGA CGACCTGCAC ATCTACTGCT CGCGCTGCAC CGCCGACCAG 
CTGGAGATCC TGAAGGTGAT GGGCATCCTG CCGTTTGCCG CGCCGTCCTG CGGGCTCATC 
ACCAAGACGG ACGCGGAGCG GCTATGCAAC GCGCTCATCT ATGGGGGCAC GTACCCGCCG 
CACTCCAAGA AGGAGCTGTA CGGCTCTATT GAGCTGGAAC TCACCGAGAA GAGCTTCAAG 
ATCTACCACG AGTGCTTCGG GAAGTGTAAG GGTCTGCTGG TCCCGGAGCT CTACACAAGT 
CCCAACGCGC CCTGCATCCA ATGCATGGAC TGCAGGCTCA TGTACCCGAC CCATAAATTC 
GTGGTGCACA GTCACAAATC GCTGGAGAAC AGGACTTGCC ATTGGGGCTT TGACTCGGCC 
AACTGGAGGG CGTATGTGCT CCTGAGTCCG GATTACACGG GGAAAGAGGA GAAAGCGCGT 
CTGGAGCAGC TCCTGGATGA CATTAAGGAG AAATTTGATT TCTCCAATAA ATACAAGCGG 
AAAGCATCAC AGGCGTCTGA TCCCATTCCT GCCAAGAAGC CCAAACACGA TGACTTTCCA 
GCACAGTCAC CCTCAGGAGA CAAAGACAAG CAACCTGATT GGCTACGGTC CCTCTCAGCG 
TCAGCCAATA AGGGGCTCAA CTGCATTCAA CCCAGACAGA GACCGTCAGC CTTCAGGCCT 
TGGTCTCCAA ACATCTCTGT TAGCGATAAA GACGGAGCAG GCAGACCCGC TAGCATGTTA 
CGAGAAAGTT TCTACAACTA CAAGAGTCTG GAAAGTGCCG TGGCCCCAAA CGTGGCTCTA 
ACACCGCCGC AGAAAGGAGG ACAGGCCTCT CCATCGGCCC CCAGCAGCTC ACATCCACAT 
GGGGCAGAGA GTGAAGGTGC GAGTCGCGGG AGGAAGAGGA GGCTGACGGG CGACACTCAA 
CCCAGTCCAC AGCCCTGCGC CGCGGCCACC GCCAGCAAAC CCCTGCCAGC CTCGCCAGAG 
GAGCACGAGT CAGATGTGGA GGTGGAGGTG GAGAGTCGAG AGGAATTAAC GTCATCGCTT 
TCCTCGCTCT CCTCCCCATC GTTTCCCTCC TCAAGCTCAG CTAAGGACTT GAGCTCTCCC 
TCAGGCCCTG TGGCCACTTT ACCAGCAGTA GTGACACCCG CCGCCGGACC CCCGGAGGGC 
CCCCTGCTGT CTGGGGGAGA GGGACACGCC ATGAATAACC TGGAGTCAGA GCTGGAGACG 
CTGAGACAGG CGCTGGACAA CGGCCTGGAC TCAAAAGAAT CCAAGGAAAA GTTTCTGCAT 
GAGATCGTGA AGATGAGAGT GAAGCAGGAG GAGAAGCTCG GATCCGCATT ACAGGCCAAA 
CGGAGCCTCC AGCAGGAGCT GGAGTTCCTC CGTGTTGCGA AGAAGGAGAA GCTCCGTGAG 
GCGACAGAAG CGAAAAGGAA CCTGCGCAAG GAGATCGAGC GCTTGCGTGC CGAAAGCGAG 
AGGAAGATGA GAGAGGCCAA CGAGTCCCGT ATGCGGCTGA AGAGAGAGCT GGAGCAGGCC 
AGACAGCTGC GGGTCTGTGA CAAGGGCTGC GAGGCCGGAC GCCTGAGGGC CAAATACTCT 
GCACAGATTG AAGACCTGCA AGTAAAGTTG CAGCACGCCG AGGCAGACCG AGAGCAGCTG 
AGAGCCGATC TGATCCATGA ACGGGAGGCG CGGGAGCACC TGGAGAGAGT GGTCAAGGAG 
CTACAGGAAC AGCTGTGGCC CAAAAGCCCC AGCAAGAATG GCACAGACGT GGCACACCCA 
CCTGCCAAAG ACTCCAACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571010.3
CDS	381..2564
Translation	METVARQSFQPHPGLQQTLKQFHLSSMISLGGPAAFSARWQHDLLFKKDGKDIPEPVLHLPPMQPPPVMPGPLFIPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNTVLRDFSLQQINSVCDDLHIYCSRCTADQLEILKVMGILPFAAPSCGLITKTDAERLCNALIYGGTYPPHSKKELYGSIELELTEKSFKIYHECFGKCKGLLVPELYTSPNAPCIQCMDCRLMYPTHKFVVHSHKSLENRTCHWGFDSANWRAYVLLSPDYTGKEEKARLEQLLDDIKEKFDFSNKYKRKASQASDPIPAKKPKHDDFPAQSPSGDKDKQPDWLRSLSASANKGLNCIQPRQRPSAFRPWSPNISVSDKDGAGRPASMLRESFYNYKSLESAVAPNVALTPPQKGGQASPSAPSSSHPHGAESEGASRGRKRRLTGDTQPSPQPCAAATASKPLPASPEEHESDVEVEVESREELTSSLSSLSSPSFPSSSSAKDLSSPSGPVATLPAVVTPAAGPPEGPLLSGGEGHAMNNLESELETLRQALDNGLDSKESKEKFLHEIVKMRVKQEEKLGSALQAKRSLQQELEFLRVAKKEKLREATEAKRNLRKEIERLRAESERKMREANESRMRLKRELEQARQLRVCDKGCEAGRLRAKYSAQIEDLQVKLQHAEADREQLRADLIHEREAREHLERVVKELQEQLWPKSPSKNGTDVAHPPAKDSNN

Target ORF information:

RefSeq Version	NM_130935.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio v-ski avian sarcoma viral oncogene homolog a (skia), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130935.3

ORF Insert Sequence:

ATGGAAACAG TAGCGCGACA GAGTTTCCAG CCTCACCCGG GACTGCAACA AACTCTCAAA 
CAGTTTCACC TGAGCTCCAT GATCTCCCTC GGAGGACCGG CCGCGTTCTC GGCCAGGTGG 
CAACACGATT TGCTCTTCAA GAAGGACGGT AAAGACATCC CGGAGCCCGT GCTGCACCTC 
CCCCCGATGC AGCCTCCTCC GGTGATGCCC GGGCCGCTCT TCATCCCGTC CGACCGCTCA 
ACCGAGAGGT GCGAGACGGT CCTGGAGGGT GAGACCATCT CGTGCTTCGT GGTGGGCGGC 
GAGAAGCGCC TGTGCCTGCC GCAGATCCTC AACACGGTGC TGCGGGATTT CTCGCTCCAG 
CAGATCAACT CTGTGTGCGA CGACCTGCAC ATCTACTGCT CGCGCTGCAC CGCCGACCAG 
CTGGAGATCC TGAAGGTGAT GGGCATCCTG CCGTTTGCCG CGCCGTCCTG CGGGCTCATC 
ACCAAGACGG ACGCGGAGCG GCTATGCAAC GCGCTCATCT ATGGGGGCAC GTACCCGCCG 
CACTCCAAGA AGGAGCTGTA CGGCTCTATT GAGCTGGAAC TCACCGAGAA GAGCTTCAAG 
ATCTACCACG AGTGCTTCGG GAAGTGTAAG GGTCTGCTGG TCCCGGAGCT CTACACAAGT 
CCCAACGCGC CCTGCATCCA ATGCATGGAC TGCAGGCTCA TGTACCCGAC CCATAAATTC 
GTGGTGCACA GTCACAAATC GCTGGAGAAC AGGACTTGCC ATTGGGGCTT TGACTCGGCC 
AACTGGAGGG CGTATGTGCT CCTGAGTCCG GATTACACGG GGAAAGAGGA GAAAGCGCGT 
CTGGAGCAGC TCCTGGATGA CATTAAGGAG AAATTTGATT TCTCCAATAA ATACAAGCGG 
AAAGCATCAC AGGCGTCTGA TCCCATTCCT GCCAAGAAGC CCAAACACGA TGACTTTCCA 
GCACAGTCAC CCTCAGGAGA CAAAGACAAG CAACCTGATT GGCTACGGTC CCTCTCAGCG 
TCAGCCAATA AGGGGCTCAA CTGCATTCAA CCCAGACAGA GACCGTCAGC CTTCAGGCCT 
TGGTCTCCAA ACATCTCTGT TAGCGATAAA GACGGAGCAG GCAGACCCGC TAGCATGTTA 
CGAGAAAGTT TCTACAACTA CAAGAGTCTG GAAAGTGCCG TGGCCCCAAA CGTGGCTCTA 
ACACCGCCGC AGAAAGGAGG ACAGGCCTCT CCATCGGCCC CCAGCAGCTC ACATCCACAT 
GGGGCAGAGA GTGAAGGTGC GAGTCGCGGG AGGAAGAGGA GGCTGACGGG CGACACTCAA 
CCCAGTCCAC AGCCCTGCGC CGCGGCCACC GCCAGCAAAC CCCTGCCAGC CTCGCCAGAG 
GAGCACGAGT CAGATGTGGA GGTGGAGGTG GAGAGTCGAG AGGAATTAAC GTCATCGCTT 
TCCTCGCTCT CCTCCCCATC GTTTCCCTCC TCAAGCTCAG CTAAGGACTT GAGCTCTCCC 
TCAGGCCCTG TGGCCACTTT ACCAGCAGTA GTGACACCCG CCGCCGGACC CCCGGAGGGC 
CCCCTGCTGT CTGGGGGAGA GGGACACGCC ATGAATAACC TGGAGTCAGA GCTGGAGACG 
CTGAGACAGG CGCTGGACAA CGGCCTGGAC TCAAAAGAAT CCAAGGAAAA GTTTCTGCAT 
GAGATCGTGA AGATGAGAGT GAAGCAGGAG GAGAAGCTCG GATCCGCATT ACAGGCCAAA 
CGGAGCCTCC AGCAGGAGCT GGAGTTCCTC CGTGTTGCGA AGAAGGAGAA GCTCCGTGAG 
GCGACAGAAG CGAAAAGGAA CCTGCGCAAG GAGATCGAGC GCTTGCGTGC CGAAAGCGAG 
AGGAAGATGA GAGAGGCCAA CGAGTCCCGT ATGCGGCTGA AGAGAGAGCT GGAGCAGGCC 
AGACAGCTGC GGGTCTGTGA CAAGGGCTGC GAGGCCGGAC GCCTGAGGGC CAAATACTCT 
GCACAGATTG AAGACCTGCA AGTAAAGTTG CAGCACGCCG AGGCAGACCG AGAGCAGCTG 
AGAGCCGATC TGATCCATGA ACGGGAGGCG CGGGAGCACC TGGAGAGAGT GGTCAAGGAG 
CTACAGGAAC AGCTGTGGCC CAAAAGCCCC AGCAAGAATG GCACAGACGT GGCACACCCA 
CCTGCCAAAG ACTCCAACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptome comparison reveals a genetic network regulating the lower temperature limit in fish.
Scientific reports628952(2016 Jun)
Hu P,Liu M,Liu Y,Wang J,Zhang D,Niu H,Jiang S,Wang J,Zhang D,Han B,Xu Q,Chen L

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Fine mapping of the 1p36 deletion syndrome identifies mutation of PRDM16 as a cause of cardiomyopathy.
American journal of human genetics93(1)67-77(2013 Jul)
Arndt AK,Schafer S,Drenckhahn JD,Sabeh MK,Plovie ER,Caliebe A,Klopocki E,Musso G,Werdich AA,Kalwa H,Heinig M,Padera RF,Wassilew K,Bluhm J,Harnack C,Martitz J,Barton PJ,Greutmann M,Berger F,Hubner N,Siebert R,Kramer HH,Cook SA,MacRae CA,Klaassen S

Mutations in the TGF-? repressor SKI cause Shprintzen-Goldberg syndrome with aortic aneurysm.
Nature genetics44(11)1249-54(2012 Nov)
Doyle AJ,Doyle JJ,Bessling SL,Maragh S,Lindsay ME,Schepers D,Gillis E,Mortier G,Homfray T,Sauls K,Norris RA,Huso ND,Leahy D,Mohr DW,Caulfield MJ,Scott AF,Destr?e A,Hennekam RC,Arn PH,Curry CJ,Van Laer L,McCallion AS,Loeys BL,Dietz HC

A fish-specific transposable element shapes the repertoire of p53 target genes in zebrafish.
PloS one7(10)e46642(2012)
Micale L,Loviglio MN,Manzoni M,Fusco C,Augello B,Migliavacca E,Cotugno G,Monti E,Borsani G,Reymond A,Merla G

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skia cDNA ORF clone, Danio rerio(zebrafish)

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