PMEL cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	PMEL
Entrez Gene ID	20431
Full Name	premelanosome protein
Synonyms	D10H12S53E,D12S53Eh,Pmel17,Si,Silv,gp100,gp87
General protein information	Preferred Names premelanosome protein Names melanocyte protein PMEL melanocyte protein pmel 17 silver locus protein
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 10 Map Location: 10 D3\|10 77.13 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_021882.4	NP_068682.2	melanocyte protein PMEL precursor
XM_006513405.4	XP_006513468.1	melanocyte protein PMEL isoform X1
XM_030244967.1	XP_030100827.1	melanocyte protein PMEL isoform X1
XM_006513405.3	XP_006513468.1	melanocyte protein PMEL isoform X1
XM_006513405.2	XP_006513468.1	melanocyte protein PMEL isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pmelb	NP_001029090.2
Danio rerio (zebrafish)	pmela	NP_001038795.1
Homo sapiens (human)	PMEL	NP_001186983.1
Pan troglodytes (chimpanzee)	PMEL	XP_001169751.2
	PMEL	XP_001113158.1
Gallus gallus (chicken)	PMEL	NP_990443.2
Canis lupus familiaris (dog)	PMEL	NP_001096686.1
Bos taurus (cattle)	PMEL	NP_001073684.2
Mus musculus (house mouse)	Pmel	NP_068682.2
Rattus norvegicus (Norway rat)	Pmel	NP_001102199.1
Xenopus tropicalis (tropical clawed frog)	pmel	XP_004911936.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of microRNA-136 on melanoma cell proliferation, apoptosis, and epithelial-mesenchymal transition by targetting PMEL through the Wnt signaling pathway.
Wang JJ, Li ZF, Li XJ, Han Z, Zhang L, Liu ZJ
Bioscience reports37(5)(2017 Oct)

The Histone Methyltransferase Ezh2 Controls Mechanisms of Adaptive Resistance to Tumor Immunotherapy.
Zingg D, Arenas-Ramirez N, Sahin D, Rosalia RA, Antunes AT, Haeusel J, Sommer L, Boyman O
Cell reports20(4)854-867(2017 Jul)

DAPL1, a susceptibility locus for age-related macular degeneration, acts as a novel suppressor of cell proliferation in the retinal pigment epithelium.
Ma X, Li H, Wang Y, Wang J, Zheng Q, Hua J, Yang J, Pan L, Lu F, Qu J, Hou L
Human molecular genetics26(9)1612-1621(2017 05)

TFAP2 paralogs regulate melanocyte differentiation in parallel with MITF.
Seberg HE, Van Otterloo E, Loftus SK, Liu H, Bonde G, Sompallae R, Gildea DE, Santana JF, Manak JR, Pavan WJ, Williams T, Cornell RA
PLoS genetics13(3)e1006636(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Prophylactic and therapeutic vaccinations of mice with MCMV-gp100KGP effectively protected mice from highly aggressive lung B16-F10 melanoma, and the protection was mediated by gp100-specific CD8(+) T cells
Title: Cytomegalovirus-Based Vaccine Expressing a Modified Tumor Antigen Induces Potent Tumor-Specific CD8(+) T-cell Response and Protects Mice from Melanoma.

Melanosome-autonomous regulation of size and number: the OA1 receptor sustains PMEL expression.
Title: Melanosome-autonomous regulation of size and number: the OA1 receptor sustains PMEL expression.

the BACE1 homologue BACE2 processes PMEL to generate functional amyloids
Title: BACE2 processes PMEL to form the melanosome amyloid matrix in pigment cells.

A long-peptide vaccine formulation of 20-mer synthetic peptide elicits gp100-specific CD8 T cells from the endogenous repertoire.
Title: Effective cooperation of monoclonal antibody and peptide vaccine for the treatment of mouse melanoma.

Despite a mild effect on visible pigmentation, inactivation of Pmel led to a substantial reduction in eumelanin content in hair, which demonstrates that PMEL has a critical role for maintaining efficient epidermal pigmentation.
Title: Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation.

The following PMEL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PMEL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18767	NM_021882.4 Latest version!	Mus musculus premelanosome protein (Pmel), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu18767	XM_030244967.1 Latest version!	Mus musculus premelanosome protein (Pmel), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu18767	XM_006513405.4 Latest version!	Mus musculus premelanosome protein (Pmel), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu18767	XM_006513405.3	Mus musculus premelanosome protein (Pmel), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu18767	XM_006513405.2	Mus musculus premelanosome protein (Pmel), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PMEL ( NM_021882.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_068682.2 Mus musculus premelanosome protein (Pmel), mRNA.

Price & Availability↑

CloneID	OMu18767
Clone ID Related Accession (Same CDS sequence)	NM_021882.4 , XM_006513405.4 , XM_030244967.1 , XM_006513405.3 , XM_006513405.2
Accession Version	NM_021882.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	melanocyte protein PMEL precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK131956.1. On Apr 6, 2007 this sequence version replaced NM_021882.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131956.1, AK012808.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGTCC AGAGAAGGAG CTTCCTTCCC GTGCTTGTGC TGAGTGCTCT GCTGGCTGTG 
GGGGCCCTAG AAGGATCCAG GAATCAGGAC TGGCTTGGTG TCCCAAGACA ACTTGTAACT 
AAAACCTGGA ACAGGCAGCT GTACCCCGAG TGGACAGAGG TGCAGGGGTC TAACTGCTGG 
AGAGGTGGCC AGGTATCTCT GAGGGTCATT AATGATGGGC CTACACTGGT TGGTGCAAAT 
GCCTCCTTTT CCATTGCCCT GCACTTCCCT GGAAGTCAAA AGGTACTACC GGATGGTCAG 
GTTATCTGGG CCAACAACAC CATCATCAAT GGGAGCCAGG TGTGGGGAGG ACAGCCAGTG 
TATCCACAGG AGCCTGATGA TGCCTGTGTC TTCCCTGACG GTGGACCCTG CCCATCTGGT 
CCTAAACCTC CGAAGAGAAG CTTTGTTTAT GTTTGGAAGA CCTGGGGAAA ATACTGGCAA 
GTTCTGGGGG GTCCAGTGTC CAGGCTGAGC ATTGCTACGG GCCACGCAAA GCTGGGCACA 
CACACAATGG AAGTGACTGT CTACCACCGA CGGGGTTCCC AGAGCTACGT GCCCCTTGCT 
CACGCCAGTT CAACCTTCAC CATTACTGAC CAGGTACCTT TCTCCGTGAG TGTGTCCCAG 
CTACAGGCCT TGGACGGAGA GACCAAGCAC TTCCTGAGAA ATCATCCTCT CATCTTTGCC 
CTTCAGCTCC ACGACCCCAG TGGTTATTTG GCCGAGGCCG ACCTCTCCTA CACATGGGAC 
TTTGGAGATG GTACTGGGAC CCTGATCTCT CGGGCACTTG ATGTCACTCA CACTTACCTG 
GAGTCGGGCT CAGTCACTGC CCAGGTGGTA CTGCAGGCTG CCATTCCTCT TGTTTCCTGT 
GGTTCCTCCC CAGTCCCGGG TACCACAGAT GGCTACATGC CAACTGCAGA AGCACCTGGA 
ACCACATCTA GGCAAGGAAC CACTACAAAA GTTGTGGGTA CTACACCTGG CCAGATGCCA 
ACTACACAGC CCTCTGGAAC CACAGTTGTA CAAATGCCAA CCACAGAGGT CACAGCTACT 
ACATCTGAGC AGATGCTGAC CTCAGCGGTC ATAGATACCA CACTGGCAGA GGTGTCAACT 
ACAGAGGGTA CAGGTACCAC ACCCACAAGG CCTTCTGGAA CCACCGTTGC ACAAGCAACA 
ACCACAGAGG GTCCAGATGC CAGCCCATTG CTGCCCACAC AAAGTTCTAC AGGGTCCATT 
AGCCCTCTAC TGGATGACAC CGACACCATA ATGCTTGTGA AGAGACAAGT TCCCCTGGAC 
TGTGTTCTAT ATCGATATGG TTCTTTCTCC CTCGCCCTGG ACATTGTCCA GGGTATTGAA 
AGTGCTGAGA TCCTGCAGGC TGTGCCATTC AGTGAAGGGG ATGCATTTGA GCTGACTGTG 
TCCTGCCAAG GCGGGCTACC CAAGGAAGCC TGTATGGACA TTTCATCACC AGGGTGCCAG 
CCCCCTGCCC AGAGGCTGTG CCAGTCTGTT CCACCGAGCC CAGACTGCCA GCTGGTTCTA 
CACCAAGTGC TGAAAGGTGG CTCAGGGACA TATTGCCTCA ATGTGTCTTT GGCTGACGCC 
AACAGCCTGG CAGTGGCCAG CACCCAACTT GTTGTTCCTG GTCAAGACGG TGGCCTTGGG 
CAGGCTCCCT TGCTTGTAGG TATCTTGCTG GTGTTGGTGG CTGTGGTCCT TGCATCTCTG 
ATACATAGGC ATAGACTTAA GAAGCAGGGC TCAGTTTCCC AAATGCCACA TGGTAGCACT 
CACTGGCTGC GCCTGCCTCC GGTCTTCCGC GCCCGCGGCC TTGGAGAAAA CAGCCCGCTC 
CTCAGTGGAC AGCAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068682.2
CDS	29..1909
Translation	MGVQRRSFLPVLVLSALLAVGALEGSRNQDWLGVPRQLVTKTWNRQLYPEWTEVQGSNCWRGGQVSLRVINDGPTLVGANASFSIALHFPGSQKVLPDGQVIWANNTIINGSQVWGGQPVYPQEPDDACVFPDGGPCPSGPKPPKRSFVYVWKTWGKYWQVLGGPVSRLSIATGHAKLGTHTMEVTVYHRRGSQSYVPLAHASSTFTITDQVPFSVSVSQLQALDGETKHFLRNHPLIFALQLHDPSGYLAEADLSYTWDFGDGTGTLISRALDVTHTYLESGSVTAQVVLQAAIPLVSCGSSPVPGTTDGYMPTAEAPGTTSRQGTTTKVVGTTPGQMPTTQPSGTTVVQMPTTEVTATTSEQMLTSAVIDTTLAEVSTTEGTGTTPTRPSGTTVAQATTTEGPDASPLLPTQSSTGSISPLLDDTDTIMLVKRQVPLDCVLYRYGSFSLALDIVQGIESAEILQAVPFSEGDAFELTVSCQGGLPKEACMDISSPGCQPPAQRLCQSVPPSPDCQLVLHQVLKGGSGTYCLNVSLADANSLAVASTQLVVPGQDGGLGQAPLLVGILLVLVAVVLASLIHRHRLKKQGSVSQMPHGSTHWLRLPPVFRARGLGENSPLLSGQQV

Target ORF information:

RefSeq Version	NM_021882.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus premelanosome protein (Pmel), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021882.4

ORF Insert Sequence:

ATGGGTGTCC AGAGAAGGAG CTTCCTTCCC GTGCTTGTGC TGAGTGCTCT GCTGGCTGTG 
GGGGCCCTAG AAGGATCCAG GAATCAGGAC TGGCTTGGTG TCCCAAGACA ACTTGTAACT 
AAAACCTGGA ACAGGCAGCT GTACCCCGAG TGGACAGAGG TGCAGGGGTC TAACTGCTGG 
AGAGGTGGCC AGGTATCTCT GAGGGTCATT AATGATGGGC CTACACTGGT TGGTGCAAAT 
GCCTCCTTTT CCATTGCCCT GCACTTCCCT GGAAGTCAAA AGGTACTACC GGATGGTCAG 
GTTATCTGGG CCAACAACAC CATCATCAAT GGGAGCCAGG TGTGGGGAGG ACAGCCAGTG 
TATCCACAGG AGCCTGATGA TGCCTGTGTC TTCCCTGACG GTGGACCCTG CCCATCTGGT 
CCTAAACCTC CGAAGAGAAG CTTTGTTTAT GTTTGGAAGA CCTGGGGAAA ATACTGGCAA 
GTTCTGGGGG GTCCAGTGTC CAGGCTGAGC ATTGCTACGG GCCACGCAAA GCTGGGCACA 
CACACAATGG AAGTGACTGT CTACCACCGA CGGGGTTCCC AGAGCTACGT GCCCCTTGCT 
CACGCCAGTT CAACCTTCAC CATTACTGAC CAGGTACCTT TCTCCGTGAG TGTGTCCCAG 
CTACAGGCCT TGGACGGAGA GACCAAGCAC TTCCTGAGAA ATCATCCTCT CATCTTTGCC 
CTTCAGCTCC ACGACCCCAG TGGTTATTTG GCCGAGGCCG ACCTCTCCTA CACATGGGAC 
TTTGGAGATG GTACTGGGAC CCTGATCTCT CGGGCACTTG ATGTCACTCA CACTTACCTG 
GAGTCGGGCT CAGTCACTGC CCAGGTGGTA CTGCAGGCTG CCATTCCTCT TGTTTCCTGT 
GGTTCCTCCC CAGTCCCGGG TACCACAGAT GGCTACATGC CAACTGCAGA AGCACCTGGA 
ACCACATCTA GGCAAGGAAC CACTACAAAA GTTGTGGGTA CTACACCTGG CCAGATGCCA 
ACTACACAGC CCTCTGGAAC CACAGTTGTA CAAATGCCAA CCACAGAGGT CACAGCTACT 
ACATCTGAGC AGATGCTGAC CTCAGCGGTC ATAGATACCA CACTGGCAGA GGTGTCAACT 
ACAGAGGGTA CAGGTACCAC ACCCACAAGG CCTTCTGGAA CCACCGTTGC ACAAGCAACA 
ACCACAGAGG GTCCAGATGC CAGCCCATTG CTGCCCACAC AAAGTTCTAC AGGGTCCATT 
AGCCCTCTAC TGGATGACAC CGACACCATA ATGCTTGTGA AGAGACAAGT TCCCCTGGAC 
TGTGTTCTAT ATCGATATGG TTCTTTCTCC CTCGCCCTGG ACATTGTCCA GGGTATTGAA 
AGTGCTGAGA TCCTGCAGGC TGTGCCATTC AGTGAAGGGG ATGCATTTGA GCTGACTGTG 
TCCTGCCAAG GCGGGCTACC CAAGGAAGCC TGTATGGACA TTTCATCACC AGGGTGCCAG 
CCCCCTGCCC AGAGGCTGTG CCAGTCTGTT CCACCGAGCC CAGACTGCCA GCTGGTTCTA 
CACCAAGTGC TGAAAGGTGG CTCAGGGACA TATTGCCTCA ATGTGTCTTT GGCTGACGCC 
AACAGCCTGG CAGTGGCCAG CACCCAACTT GTTGTTCCTG GTCAAGACGG TGGCCTTGGG 
CAGGCTCCCT TGCTTGTAGG TATCTTGCTG GTGTTGGTGG CTGTGGTCCT TGCATCTCTG 
ATACATAGGC ATAGACTTAA GAAGCAGGGC TCAGTTTCCC AAATGCCACA TGGTAGCACT 
CACTGGCTGC GCCTGCCTCC GGTCTTCCGC GCCCGCGGCC TTGGAGAAAA CAGCCCGCTC 
CTCAGTGGAC AGCAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PMEL ( XM_006513405.4 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006513468.1 Mus musculus premelanosome protein (Pmel), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18767
Clone ID Related Accession (Same CDS sequence)	NM_021882.4 , XM_006513405.4 , XM_030244967.1 , XM_006513405.3 , XM_006513405.2
Accession Version	XM_006513405.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	melanocyte protein PMEL isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513405.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGTCC AGAGAAGGAG CTTCCTTCCC GTGCTTGTGC TGAGTGCTCT GCTGGCTGTG 
GGGGCCCTAG AAGGATCCAG GAATCAGGAC TGGCTTGGTG TCCCAAGACA ACTTGTAACT 
AAAACCTGGA ACAGGCAGCT GTACCCCGAG TGGACAGAGG TGCAGGGGTC TAACTGCTGG 
AGAGGTGGCC AGGTATCTCT GAGGGTCATT AATGATGGGC CTACACTGGT TGGTGCAAAT 
GCCTCCTTTT CCATTGCCCT GCACTTCCCT GGAAGTCAAA AGGTACTACC GGATGGTCAG 
GTTATCTGGG CCAACAACAC CATCATCAAT GGGAGCCAGG TGTGGGGAGG ACAGCCAGTG 
TATCCACAGG AGCCTGATGA TGCCTGTGTC TTCCCTGACG GTGGACCCTG CCCATCTGGT 
CCTAAACCTC CGAAGAGAAG CTTTGTTTAT GTTTGGAAGA CCTGGGGAAA ATACTGGCAA 
GTTCTGGGGG GTCCAGTGTC CAGGCTGAGC ATTGCTACGG GCCACGCAAA GCTGGGCACA 
CACACAATGG AAGTGACTGT CTACCACCGA CGGGGTTCCC AGAGCTACGT GCCCCTTGCT 
CACGCCAGTT CAACCTTCAC CATTACTGAC CAGGTACCTT TCTCCGTGAG TGTGTCCCAG 
CTACAGGCCT TGGACGGAGA GACCAAGCAC TTCCTGAGAA ATCATCCTCT CATCTTTGCC 
CTTCAGCTCC ACGACCCCAG TGGTTATTTG GCCGAGGCCG ACCTCTCCTA CACATGGGAC 
TTTGGAGATG GTACTGGGAC CCTGATCTCT CGGGCACTTG ATGTCACTCA CACTTACCTG 
GAGTCGGGCT CAGTCACTGC CCAGGTGGTA CTGCAGGCTG CCATTCCTCT TGTTTCCTGT 
GGTTCCTCCC CAGTCCCGGG TACCACAGAT GGCTACATGC CAACTGCAGA AGCACCTGGA 
ACCACATCTA GGCAAGGAAC CACTACAAAA GTTGTGGGTA CTACACCTGG CCAGATGCCA 
ACTACACAGC CCTCTGGAAC CACAGTTGTA CAAATGCCAA CCACAGAGGT CACAGCTACT 
ACATCTGAGC AGATGCTGAC CTCAGCGGTC ATAGATACCA CACTGGCAGA GGTGTCAACT 
ACAGAGGGTA CAGGTACCAC ACCCACAAGG CCTTCTGGAA CCACCGTTGC ACAAGCAACA 
ACCACAGAGG GTCCAGATGC CAGCCCATTG CTGCCCACAC AAAGTTCTAC AGGGTCCATT 
AGCCCTCTAC TGGATGACAC CGACACCATA ATGCTTGTGA AGAGACAAGT TCCCCTGGAC 
TGTGTTCTAT ATCGATATGG TTCTTTCTCC CTCGCCCTGG ACATTGTCCA GGGTATTGAA 
AGTGCTGAGA TCCTGCAGGC TGTGCCATTC AGTGAAGGGG ATGCATTTGA GCTGACTGTG 
TCCTGCCAAG GCGGGCTACC CAAGGAAGCC TGTATGGACA TTTCATCACC AGGGTGCCAG 
CCCCCTGCCC AGAGGCTGTG CCAGTCTGTT CCACCGAGCC CAGACTGCCA GCTGGTTCTA 
CACCAAGTGC TGAAAGGTGG CTCAGGGACA TATTGCCTCA ATGTGTCTTT GGCTGACGCC 
AACAGCCTGG CAGTGGCCAG CACCCAACTT GTTGTTCCTG GTCAAGACGG TGGCCTTGGG 
CAGGCTCCCT TGCTTGTAGG TATCTTGCTG GTGTTGGTGG CTGTGGTCCT TGCATCTCTG 
ATACATAGGC ATAGACTTAA GAAGCAGGGC TCAGTTTCCC AAATGCCACA TGGTAGCACT 
CACTGGCTGC GCCTGCCTCC GGTCTTCCGC GCCCGCGGCC TTGGAGAAAA CAGCCCGCTC 
CTCAGTGGAC AGCAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513468.1
CDS	277..2157
Translation	MGVQRRSFLPVLVLSALLAVGALEGSRNQDWLGVPRQLVTKTWNRQLYPEWTEVQGSNCWRGGQVSLRVINDGPTLVGANASFSIALHFPGSQKVLPDGQVIWANNTIINGSQVWGGQPVYPQEPDDACVFPDGGPCPSGPKPPKRSFVYVWKTWGKYWQVLGGPVSRLSIATGHAKLGTHTMEVTVYHRRGSQSYVPLAHASSTFTITDQVPFSVSVSQLQALDGETKHFLRNHPLIFALQLHDPSGYLAEADLSYTWDFGDGTGTLISRALDVTHTYLESGSVTAQVVLQAAIPLVSCGSSPVPGTTDGYMPTAEAPGTTSRQGTTTKVVGTTPGQMPTTQPSGTTVVQMPTTEVTATTSEQMLTSAVIDTTLAEVSTTEGTGTTPTRPSGTTVAQATTTEGPDASPLLPTQSSTGSISPLLDDTDTIMLVKRQVPLDCVLYRYGSFSLALDIVQGIESAEILQAVPFSEGDAFELTVSCQGGLPKEACMDISSPGCQPPAQRLCQSVPPSPDCQLVLHQVLKGGSGTYCLNVSLADANSLAVASTQLVVPGQDGGLGQAPLLVGILLVLVAVVLASLIHRHRLKKQGSVSQMPHGSTHWLRLPPVFRARGLGENSPLLSGQQV

Target ORF information:

RefSeq Version	XM_006513405.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus premelanosome protein (Pmel), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513405.4

ORF Insert Sequence:

ATGGGTGTCC AGAGAAGGAG CTTCCTTCCC GTGCTTGTGC TGAGTGCTCT GCTGGCTGTG 
GGGGCCCTAG AAGGATCCAG GAATCAGGAC TGGCTTGGTG TCCCAAGACA ACTTGTAACT 
AAAACCTGGA ACAGGCAGCT GTACCCCGAG TGGACAGAGG TGCAGGGGTC TAACTGCTGG 
AGAGGTGGCC AGGTATCTCT GAGGGTCATT AATGATGGGC CTACACTGGT TGGTGCAAAT 
GCCTCCTTTT CCATTGCCCT GCACTTCCCT GGAAGTCAAA AGGTACTACC GGATGGTCAG 
GTTATCTGGG CCAACAACAC CATCATCAAT GGGAGCCAGG TGTGGGGAGG ACAGCCAGTG 
TATCCACAGG AGCCTGATGA TGCCTGTGTC TTCCCTGACG GTGGACCCTG CCCATCTGGT 
CCTAAACCTC CGAAGAGAAG CTTTGTTTAT GTTTGGAAGA CCTGGGGAAA ATACTGGCAA 
GTTCTGGGGG GTCCAGTGTC CAGGCTGAGC ATTGCTACGG GCCACGCAAA GCTGGGCACA 
CACACAATGG AAGTGACTGT CTACCACCGA CGGGGTTCCC AGAGCTACGT GCCCCTTGCT 
CACGCCAGTT CAACCTTCAC CATTACTGAC CAGGTACCTT TCTCCGTGAG TGTGTCCCAG 
CTACAGGCCT TGGACGGAGA GACCAAGCAC TTCCTGAGAA ATCATCCTCT CATCTTTGCC 
CTTCAGCTCC ACGACCCCAG TGGTTATTTG GCCGAGGCCG ACCTCTCCTA CACATGGGAC 
TTTGGAGATG GTACTGGGAC CCTGATCTCT CGGGCACTTG ATGTCACTCA CACTTACCTG 
GAGTCGGGCT CAGTCACTGC CCAGGTGGTA CTGCAGGCTG CCATTCCTCT TGTTTCCTGT 
GGTTCCTCCC CAGTCCCGGG TACCACAGAT GGCTACATGC CAACTGCAGA AGCACCTGGA 
ACCACATCTA GGCAAGGAAC CACTACAAAA GTTGTGGGTA CTACACCTGG CCAGATGCCA 
ACTACACAGC CCTCTGGAAC CACAGTTGTA CAAATGCCAA CCACAGAGGT CACAGCTACT 
ACATCTGAGC AGATGCTGAC CTCAGCGGTC ATAGATACCA CACTGGCAGA GGTGTCAACT 
ACAGAGGGTA CAGGTACCAC ACCCACAAGG CCTTCTGGAA CCACCGTTGC ACAAGCAACA 
ACCACAGAGG GTCCAGATGC CAGCCCATTG CTGCCCACAC AAAGTTCTAC AGGGTCCATT 
AGCCCTCTAC TGGATGACAC CGACACCATA ATGCTTGTGA AGAGACAAGT TCCCCTGGAC 
TGTGTTCTAT ATCGATATGG TTCTTTCTCC CTCGCCCTGG ACATTGTCCA GGGTATTGAA 
AGTGCTGAGA TCCTGCAGGC TGTGCCATTC AGTGAAGGGG ATGCATTTGA GCTGACTGTG 
TCCTGCCAAG GCGGGCTACC CAAGGAAGCC TGTATGGACA TTTCATCACC AGGGTGCCAG 
CCCCCTGCCC AGAGGCTGTG CCAGTCTGTT CCACCGAGCC CAGACTGCCA GCTGGTTCTA 
CACCAAGTGC TGAAAGGTGG CTCAGGGACA TATTGCCTCA ATGTGTCTTT GGCTGACGCC 
AACAGCCTGG CAGTGGCCAG CACCCAACTT GTTGTTCCTG GTCAAGACGG TGGCCTTGGG 
CAGGCTCCCT TGCTTGTAGG TATCTTGCTG GTGTTGGTGG CTGTGGTCCT TGCATCTCTG 
ATACATAGGC ATAGACTTAA GAAGCAGGGC TCAGTTTCCC AAATGCCACA TGGTAGCACT 
CACTGGCTGC GCCTGCCTCC GGTCTTCCGC GCCCGCGGCC TTGGAGAAAA CAGCCCGCTC 
CTCAGTGGAC AGCAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PMEL ( XM_030244967.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030100827.1 Mus musculus premelanosome protein (Pmel), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu18767
Clone ID Related Accession (Same CDS sequence)	NM_021882.4 , XM_006513405.4 , XM_030244967.1 , XM_006513405.3 , XM_006513405.2
Accession Version	XM_030244967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	melanocyte protein PMEL isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGTCC AGAGAAGGAG CTTCCTTCCC GTGCTTGTGC TGAGTGCTCT GCTGGCTGTG 
GGGGCCCTAG AAGGATCCAG GAATCAGGAC TGGCTTGGTG TCCCAAGACA ACTTGTAACT 
AAAACCTGGA ACAGGCAGCT GTACCCCGAG TGGACAGAGG TGCAGGGGTC TAACTGCTGG 
AGAGGTGGCC AGGTATCTCT GAGGGTCATT AATGATGGGC CTACACTGGT TGGTGCAAAT 
GCCTCCTTTT CCATTGCCCT GCACTTCCCT GGAAGTCAAA AGGTACTACC GGATGGTCAG 
GTTATCTGGG CCAACAACAC CATCATCAAT GGGAGCCAGG TGTGGGGAGG ACAGCCAGTG 
TATCCACAGG AGCCTGATGA TGCCTGTGTC TTCCCTGACG GTGGACCCTG CCCATCTGGT 
CCTAAACCTC CGAAGAGAAG CTTTGTTTAT GTTTGGAAGA CCTGGGGAAA ATACTGGCAA 
GTTCTGGGGG GTCCAGTGTC CAGGCTGAGC ATTGCTACGG GCCACGCAAA GCTGGGCACA 
CACACAATGG AAGTGACTGT CTACCACCGA CGGGGTTCCC AGAGCTACGT GCCCCTTGCT 
CACGCCAGTT CAACCTTCAC CATTACTGAC CAGGTACCTT TCTCCGTGAG TGTGTCCCAG 
CTACAGGCCT TGGACGGAGA GACCAAGCAC TTCCTGAGAA ATCATCCTCT CATCTTTGCC 
CTTCAGCTCC ACGACCCCAG TGGTTATTTG GCCGAGGCCG ACCTCTCCTA CACATGGGAC 
TTTGGAGATG GTACTGGGAC CCTGATCTCT CGGGCACTTG ATGTCACTCA CACTTACCTG 
GAGTCGGGCT CAGTCACTGC CCAGGTGGTA CTGCAGGCTG CCATTCCTCT TGTTTCCTGT 
GGTTCCTCCC CAGTCCCGGG TACCACAGAT GGCTACATGC CAACTGCAGA AGCACCTGGA 
ACCACATCTA GGCAAGGAAC CACTACAAAA GTTGTGGGTA CTACACCTGG CCAGATGCCA 
ACTACACAGC CCTCTGGAAC CACAGTTGTA CAAATGCCAA CCACAGAGGT CACAGCTACT 
ACATCTGAGC AGATGCTGAC CTCAGCGGTC ATAGATACCA CACTGGCAGA GGTGTCAACT 
ACAGAGGGTA CAGGTACCAC ACCCACAAGG CCTTCTGGAA CCACCGTTGC ACAAGCAACA 
ACCACAGAGG GTCCAGATGC CAGCCCATTG CTGCCCACAC AAAGTTCTAC AGGGTCCATT 
AGCCCTCTAC TGGATGACAC CGACACCATA ATGCTTGTGA AGAGACAAGT TCCCCTGGAC 
TGTGTTCTAT ATCGATATGG TTCTTTCTCC CTCGCCCTGG ACATTGTCCA GGGTATTGAA 
AGTGCTGAGA TCCTGCAGGC TGTGCCATTC AGTGAAGGGG ATGCATTTGA GCTGACTGTG 
TCCTGCCAAG GCGGGCTACC CAAGGAAGCC TGTATGGACA TTTCATCACC AGGGTGCCAG 
CCCCCTGCCC AGAGGCTGTG CCAGTCTGTT CCACCGAGCC CAGACTGCCA GCTGGTTCTA 
CACCAAGTGC TGAAAGGTGG CTCAGGGACA TATTGCCTCA ATGTGTCTTT GGCTGACGCC 
AACAGCCTGG CAGTGGCCAG CACCCAACTT GTTGTTCCTG GTCAAGACGG TGGCCTTGGG 
CAGGCTCCCT TGCTTGTAGG TATCTTGCTG GTGTTGGTGG CTGTGGTCCT TGCATCTCTG 
ATACATAGGC ATAGACTTAA GAAGCAGGGC TCAGTTTCCC AAATGCCACA TGGTAGCACT 
CACTGGCTGC GCCTGCCTCC GGTCTTCCGC GCCCGCGGCC TTGGAGAAAA CAGCCCGCTC 
CTCAGTGGAC AGCAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100827.1
CDS	777..2657
Translation	MGVQRRSFLPVLVLSALLAVGALEGSRNQDWLGVPRQLVTKTWNRQLYPEWTEVQGSNCWRGGQVSLRVINDGPTLVGANASFSIALHFPGSQKVLPDGQVIWANNTIINGSQVWGGQPVYPQEPDDACVFPDGGPCPSGPKPPKRSFVYVWKTWGKYWQVLGGPVSRLSIATGHAKLGTHTMEVTVYHRRGSQSYVPLAHASSTFTITDQVPFSVSVSQLQALDGETKHFLRNHPLIFALQLHDPSGYLAEADLSYTWDFGDGTGTLISRALDVTHTYLESGSVTAQVVLQAAIPLVSCGSSPVPGTTDGYMPTAEAPGTTSRQGTTTKVVGTTPGQMPTTQPSGTTVVQMPTTEVTATTSEQMLTSAVIDTTLAEVSTTEGTGTTPTRPSGTTVAQATTTEGPDASPLLPTQSSTGSISPLLDDTDTIMLVKRQVPLDCVLYRYGSFSLALDIVQGIESAEILQAVPFSEGDAFELTVSCQGGLPKEACMDISSPGCQPPAQRLCQSVPPSPDCQLVLHQVLKGGSGTYCLNVSLADANSLAVASTQLVVPGQDGGLGQAPLLVGILLVLVAVVLASLIHRHRLKKQGSVSQMPHGSTHWLRLPPVFRARGLGENSPLLSGQQV

Target ORF information:

RefSeq Version	XM_030244967.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus premelanosome protein (Pmel), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244967.1

ORF Insert Sequence:

ATGGGTGTCC AGAGAAGGAG CTTCCTTCCC GTGCTTGTGC TGAGTGCTCT GCTGGCTGTG 
GGGGCCCTAG AAGGATCCAG GAATCAGGAC TGGCTTGGTG TCCCAAGACA ACTTGTAACT 
AAAACCTGGA ACAGGCAGCT GTACCCCGAG TGGACAGAGG TGCAGGGGTC TAACTGCTGG 
AGAGGTGGCC AGGTATCTCT GAGGGTCATT AATGATGGGC CTACACTGGT TGGTGCAAAT 
GCCTCCTTTT CCATTGCCCT GCACTTCCCT GGAAGTCAAA AGGTACTACC GGATGGTCAG 
GTTATCTGGG CCAACAACAC CATCATCAAT GGGAGCCAGG TGTGGGGAGG ACAGCCAGTG 
TATCCACAGG AGCCTGATGA TGCCTGTGTC TTCCCTGACG GTGGACCCTG CCCATCTGGT 
CCTAAACCTC CGAAGAGAAG CTTTGTTTAT GTTTGGAAGA CCTGGGGAAA ATACTGGCAA 
GTTCTGGGGG GTCCAGTGTC CAGGCTGAGC ATTGCTACGG GCCACGCAAA GCTGGGCACA 
CACACAATGG AAGTGACTGT CTACCACCGA CGGGGTTCCC AGAGCTACGT GCCCCTTGCT 
CACGCCAGTT CAACCTTCAC CATTACTGAC CAGGTACCTT TCTCCGTGAG TGTGTCCCAG 
CTACAGGCCT TGGACGGAGA GACCAAGCAC TTCCTGAGAA ATCATCCTCT CATCTTTGCC 
CTTCAGCTCC ACGACCCCAG TGGTTATTTG GCCGAGGCCG ACCTCTCCTA CACATGGGAC 
TTTGGAGATG GTACTGGGAC CCTGATCTCT CGGGCACTTG ATGTCACTCA CACTTACCTG 
GAGTCGGGCT CAGTCACTGC CCAGGTGGTA CTGCAGGCTG CCATTCCTCT TGTTTCCTGT 
GGTTCCTCCC CAGTCCCGGG TACCACAGAT GGCTACATGC CAACTGCAGA AGCACCTGGA 
ACCACATCTA GGCAAGGAAC CACTACAAAA GTTGTGGGTA CTACACCTGG CCAGATGCCA 
ACTACACAGC CCTCTGGAAC CACAGTTGTA CAAATGCCAA CCACAGAGGT CACAGCTACT 
ACATCTGAGC AGATGCTGAC CTCAGCGGTC ATAGATACCA CACTGGCAGA GGTGTCAACT 
ACAGAGGGTA CAGGTACCAC ACCCACAAGG CCTTCTGGAA CCACCGTTGC ACAAGCAACA 
ACCACAGAGG GTCCAGATGC CAGCCCATTG CTGCCCACAC AAAGTTCTAC AGGGTCCATT 
AGCCCTCTAC TGGATGACAC CGACACCATA ATGCTTGTGA AGAGACAAGT TCCCCTGGAC 
TGTGTTCTAT ATCGATATGG TTCTTTCTCC CTCGCCCTGG ACATTGTCCA GGGTATTGAA 
AGTGCTGAGA TCCTGCAGGC TGTGCCATTC AGTGAAGGGG ATGCATTTGA GCTGACTGTG 
TCCTGCCAAG GCGGGCTACC CAAGGAAGCC TGTATGGACA TTTCATCACC AGGGTGCCAG 
CCCCCTGCCC AGAGGCTGTG CCAGTCTGTT CCACCGAGCC CAGACTGCCA GCTGGTTCTA 
CACCAAGTGC TGAAAGGTGG CTCAGGGACA TATTGCCTCA ATGTGTCTTT GGCTGACGCC 
AACAGCCTGG CAGTGGCCAG CACCCAACTT GTTGTTCCTG GTCAAGACGG TGGCCTTGGG 
CAGGCTCCCT TGCTTGTAGG TATCTTGCTG GTGTTGGTGG CTGTGGTCCT TGCATCTCTG 
ATACATAGGC ATAGACTTAA GAAGCAGGGC TCAGTTTCCC AAATGCCACA TGGTAGCACT 
CACTGGCTGC GCCTGCCTCC GGTCTTCCGC GCCCGCGGCC TTGGAGAAAA CAGCCCGCTC 
CTCAGTGGAC AGCAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PMEL ( XM_006513405.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006513468.1 Mus musculus premelanosome protein (Pmel), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18767
Clone ID Related Accession (Same CDS sequence)	NM_021882.4 , XM_006513405.4 , XM_030244967.1 , XM_006513405.3 , XM_006513405.2
Accession Version	XM_006513405.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	melanocyte protein PMEL isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513405.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGTCC AGAGAAGGAG CTTCCTTCCC GTGCTTGTGC TGAGTGCTCT GCTGGCTGTG 
GGGGCCCTAG AAGGATCCAG GAATCAGGAC TGGCTTGGTG TCCCAAGACA ACTTGTAACT 
AAAACCTGGA ACAGGCAGCT GTACCCCGAG TGGACAGAGG TGCAGGGGTC TAACTGCTGG 
AGAGGTGGCC AGGTATCTCT GAGGGTCATT AATGATGGGC CTACACTGGT TGGTGCAAAT 
GCCTCCTTTT CCATTGCCCT GCACTTCCCT GGAAGTCAAA AGGTACTACC GGATGGTCAG 
GTTATCTGGG CCAACAACAC CATCATCAAT GGGAGCCAGG TGTGGGGAGG ACAGCCAGTG 
TATCCACAGG AGCCTGATGA TGCCTGTGTC TTCCCTGACG GTGGACCCTG CCCATCTGGT 
CCTAAACCTC CGAAGAGAAG CTTTGTTTAT GTTTGGAAGA CCTGGGGAAA ATACTGGCAA 
GTTCTGGGGG GTCCAGTGTC CAGGCTGAGC ATTGCTACGG GCCACGCAAA GCTGGGCACA 
CACACAATGG AAGTGACTGT CTACCACCGA CGGGGTTCCC AGAGCTACGT GCCCCTTGCT 
CACGCCAGTT CAACCTTCAC CATTACTGAC CAGGTACCTT TCTCCGTGAG TGTGTCCCAG 
CTACAGGCCT TGGACGGAGA GACCAAGCAC TTCCTGAGAA ATCATCCTCT CATCTTTGCC 
CTTCAGCTCC ACGACCCCAG TGGTTATTTG GCCGAGGCCG ACCTCTCCTA CACATGGGAC 
TTTGGAGATG GTACTGGGAC CCTGATCTCT CGGGCACTTG ATGTCACTCA CACTTACCTG 
GAGTCGGGCT CAGTCACTGC CCAGGTGGTA CTGCAGGCTG CCATTCCTCT TGTTTCCTGT 
GGTTCCTCCC CAGTCCCGGG TACCACAGAT GGCTACATGC CAACTGCAGA AGCACCTGGA 
ACCACATCTA GGCAAGGAAC CACTACAAAA GTTGTGGGTA CTACACCTGG CCAGATGCCA 
ACTACACAGC CCTCTGGAAC CACAGTTGTA CAAATGCCAA CCACAGAGGT CACAGCTACT 
ACATCTGAGC AGATGCTGAC CTCAGCGGTC ATAGATACCA CACTGGCAGA GGTGTCAACT 
ACAGAGGGTA CAGGTACCAC ACCCACAAGG CCTTCTGGAA CCACCGTTGC ACAAGCAACA 
ACCACAGAGG GTCCAGATGC CAGCCCATTG CTGCCCACAC AAAGTTCTAC AGGGTCCATT 
AGCCCTCTAC TGGATGACAC CGACACCATA ATGCTTGTGA AGAGACAAGT TCCCCTGGAC 
TGTGTTCTAT ATCGATATGG TTCTTTCTCC CTCGCCCTGG ACATTGTCCA GGGTATTGAA 
AGTGCTGAGA TCCTGCAGGC TGTGCCATTC AGTGAAGGGG ATGCATTTGA GCTGACTGTG 
TCCTGCCAAG GCGGGCTACC CAAGGAAGCC TGTATGGACA TTTCATCACC AGGGTGCCAG 
CCCCCTGCCC AGAGGCTGTG CCAGTCTGTT CCACCGAGCC CAGACTGCCA GCTGGTTCTA 
CACCAAGTGC TGAAAGGTGG CTCAGGGACA TATTGCCTCA ATGTGTCTTT GGCTGACGCC 
AACAGCCTGG CAGTGGCCAG CACCCAACTT GTTGTTCCTG GTCAAGACGG TGGCCTTGGG 
CAGGCTCCCT TGCTTGTAGG TATCTTGCTG GTGTTGGTGG CTGTGGTCCT TGCATCTCTG 
ATACATAGGC ATAGACTTAA GAAGCAGGGC TCAGTTTCCC AAATGCCACA TGGTAGCACT 
CACTGGCTGC GCCTGCCTCC GGTCTTCCGC GCCCGCGGCC TTGGAGAAAA CAGCCCGCTC 
CTCAGTGGAC AGCAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513468.1
CDS	192..2072
Translation	MGVQRRSFLPVLVLSALLAVGALEGSRNQDWLGVPRQLVTKTWNRQLYPEWTEVQGSNCWRGGQVSLRVINDGPTLVGANASFSIALHFPGSQKVLPDGQVIWANNTIINGSQVWGGQPVYPQEPDDACVFPDGGPCPSGPKPPKRSFVYVWKTWGKYWQVLGGPVSRLSIATGHAKLGTHTMEVTVYHRRGSQSYVPLAHASSTFTITDQVPFSVSVSQLQALDGETKHFLRNHPLIFALQLHDPSGYLAEADLSYTWDFGDGTGTLISRALDVTHTYLESGSVTAQVVLQAAIPLVSCGSSPVPGTTDGYMPTAEAPGTTSRQGTTTKVVGTTPGQMPTTQPSGTTVVQMPTTEVTATTSEQMLTSAVIDTTLAEVSTTEGTGTTPTRPSGTTVAQATTTEGPDASPLLPTQSSTGSISPLLDDTDTIMLVKRQVPLDCVLYRYGSFSLALDIVQGIESAEILQAVPFSEGDAFELTVSCQGGLPKEACMDISSPGCQPPAQRLCQSVPPSPDCQLVLHQVLKGGSGTYCLNVSLADANSLAVASTQLVVPGQDGGLGQAPLLVGILLVLVAVVLASLIHRHRLKKQGSVSQMPHGSTHWLRLPPVFRARGLGENSPLLSGQQV

Target ORF information:

RefSeq Version	XM_006513405.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus premelanosome protein (Pmel), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513405.3

ORF Insert Sequence:

ATGGGTGTCC AGAGAAGGAG CTTCCTTCCC GTGCTTGTGC TGAGTGCTCT GCTGGCTGTG 
GGGGCCCTAG AAGGATCCAG GAATCAGGAC TGGCTTGGTG TCCCAAGACA ACTTGTAACT 
AAAACCTGGA ACAGGCAGCT GTACCCCGAG TGGACAGAGG TGCAGGGGTC TAACTGCTGG 
AGAGGTGGCC AGGTATCTCT GAGGGTCATT AATGATGGGC CTACACTGGT TGGTGCAAAT 
GCCTCCTTTT CCATTGCCCT GCACTTCCCT GGAAGTCAAA AGGTACTACC GGATGGTCAG 
GTTATCTGGG CCAACAACAC CATCATCAAT GGGAGCCAGG TGTGGGGAGG ACAGCCAGTG 
TATCCACAGG AGCCTGATGA TGCCTGTGTC TTCCCTGACG GTGGACCCTG CCCATCTGGT 
CCTAAACCTC CGAAGAGAAG CTTTGTTTAT GTTTGGAAGA CCTGGGGAAA ATACTGGCAA 
GTTCTGGGGG GTCCAGTGTC CAGGCTGAGC ATTGCTACGG GCCACGCAAA GCTGGGCACA 
CACACAATGG AAGTGACTGT CTACCACCGA CGGGGTTCCC AGAGCTACGT GCCCCTTGCT 
CACGCCAGTT CAACCTTCAC CATTACTGAC CAGGTACCTT TCTCCGTGAG TGTGTCCCAG 
CTACAGGCCT TGGACGGAGA GACCAAGCAC TTCCTGAGAA ATCATCCTCT CATCTTTGCC 
CTTCAGCTCC ACGACCCCAG TGGTTATTTG GCCGAGGCCG ACCTCTCCTA CACATGGGAC 
TTTGGAGATG GTACTGGGAC CCTGATCTCT CGGGCACTTG ATGTCACTCA CACTTACCTG 
GAGTCGGGCT CAGTCACTGC CCAGGTGGTA CTGCAGGCTG CCATTCCTCT TGTTTCCTGT 
GGTTCCTCCC CAGTCCCGGG TACCACAGAT GGCTACATGC CAACTGCAGA AGCACCTGGA 
ACCACATCTA GGCAAGGAAC CACTACAAAA GTTGTGGGTA CTACACCTGG CCAGATGCCA 
ACTACACAGC CCTCTGGAAC CACAGTTGTA CAAATGCCAA CCACAGAGGT CACAGCTACT 
ACATCTGAGC AGATGCTGAC CTCAGCGGTC ATAGATACCA CACTGGCAGA GGTGTCAACT 
ACAGAGGGTA CAGGTACCAC ACCCACAAGG CCTTCTGGAA CCACCGTTGC ACAAGCAACA 
ACCACAGAGG GTCCAGATGC CAGCCCATTG CTGCCCACAC AAAGTTCTAC AGGGTCCATT 
AGCCCTCTAC TGGATGACAC CGACACCATA ATGCTTGTGA AGAGACAAGT TCCCCTGGAC 
TGTGTTCTAT ATCGATATGG TTCTTTCTCC CTCGCCCTGG ACATTGTCCA GGGTATTGAA 
AGTGCTGAGA TCCTGCAGGC TGTGCCATTC AGTGAAGGGG ATGCATTTGA GCTGACTGTG 
TCCTGCCAAG GCGGGCTACC CAAGGAAGCC TGTATGGACA TTTCATCACC AGGGTGCCAG 
CCCCCTGCCC AGAGGCTGTG CCAGTCTGTT CCACCGAGCC CAGACTGCCA GCTGGTTCTA 
CACCAAGTGC TGAAAGGTGG CTCAGGGACA TATTGCCTCA ATGTGTCTTT GGCTGACGCC 
AACAGCCTGG CAGTGGCCAG CACCCAACTT GTTGTTCCTG GTCAAGACGG TGGCCTTGGG 
CAGGCTCCCT TGCTTGTAGG TATCTTGCTG GTGTTGGTGG CTGTGGTCCT TGCATCTCTG 
ATACATAGGC ATAGACTTAA GAAGCAGGGC TCAGTTTCCC AAATGCCACA TGGTAGCACT 
CACTGGCTGC GCCTGCCTCC GGTCTTCCGC GCCCGCGGCC TTGGAGAAAA CAGCCCGCTC 
CTCAGTGGAC AGCAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PMEL ( XM_006513405.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006513468.1 Mus musculus premelanosome protein (Pmel), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18767
Clone ID Related Accession (Same CDS sequence)	NM_021882.4 , XM_006513405.4 , XM_030244967.1 , XM_006513405.3 , XM_006513405.2
Accession Version	XM_006513405.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	melanocyte protein PMEL isoform X1
Comment

ATGGGTGTCC AGAGAAGGAG CTTCCTTCCC GTGCTTGTGC TGAGTGCTCT GCTGGCTGTG 
GGGGCCCTAG AAGGATCCAG GAATCAGGAC TGGCTTGGTG TCCCAAGACA ACTTGTAACT 
AAAACCTGGA ACAGGCAGCT GTACCCCGAG TGGACAGAGG TGCAGGGGTC TAACTGCTGG 
AGAGGTGGCC AGGTATCTCT GAGGGTCATT AATGATGGGC CTACACTGGT TGGTGCAAAT 
GCCTCCTTTT CCATTGCCCT GCACTTCCCT GGAAGTCAAA AGGTACTACC GGATGGTCAG 
GTTATCTGGG CCAACAACAC CATCATCAAT GGGAGCCAGG TGTGGGGAGG ACAGCCAGTG 
TATCCACAGG AGCCTGATGA TGCCTGTGTC TTCCCTGACG GTGGACCCTG CCCATCTGGT 
CCTAAACCTC CGAAGAGAAG CTTTGTTTAT GTTTGGAAGA CCTGGGGAAA ATACTGGCAA 
GTTCTGGGGG GTCCAGTGTC CAGGCTGAGC ATTGCTACGG GCCACGCAAA GCTGGGCACA 
CACACAATGG AAGTGACTGT CTACCACCGA CGGGGTTCCC AGAGCTACGT GCCCCTTGCT 
CACGCCAGTT CAACCTTCAC CATTACTGAC CAGGTACCTT TCTCCGTGAG TGTGTCCCAG 
CTACAGGCCT TGGACGGAGA GACCAAGCAC TTCCTGAGAA ATCATCCTCT CATCTTTGCC 
CTTCAGCTCC ACGACCCCAG TGGTTATTTG GCCGAGGCCG ACCTCTCCTA CACATGGGAC 
TTTGGAGATG GTACTGGGAC CCTGATCTCT CGGGCACTTG ATGTCACTCA CACTTACCTG 
GAGTCGGGCT CAGTCACTGC CCAGGTGGTA CTGCAGGCTG CCATTCCTCT TGTTTCCTGT 
GGTTCCTCCC CAGTCCCGGG TACCACAGAT GGCTACATGC CAACTGCAGA AGCACCTGGA 
ACCACATCTA GGCAAGGAAC CACTACAAAA GTTGTGGGTA CTACACCTGG CCAGATGCCA 
ACTACACAGC CCTCTGGAAC CACAGTTGTA CAAATGCCAA CCACAGAGGT CACAGCTACT 
ACATCTGAGC AGATGCTGAC CTCAGCGGTC ATAGATACCA CACTGGCAGA GGTGTCAACT 
ACAGAGGGTA CAGGTACCAC ACCCACAAGG CCTTCTGGAA CCACCGTTGC ACAAGCAACA 
ACCACAGAGG GTCCAGATGC CAGCCCATTG CTGCCCACAC AAAGTTCTAC AGGGTCCATT 
AGCCCTCTAC TGGATGACAC CGACACCATA ATGCTTGTGA AGAGACAAGT TCCCCTGGAC 
TGTGTTCTAT ATCGATATGG TTCTTTCTCC CTCGCCCTGG ACATTGTCCA GGGTATTGAA 
AGTGCTGAGA TCCTGCAGGC TGTGCCATTC AGTGAAGGGG ATGCATTTGA GCTGACTGTG 
TCCTGCCAAG GCGGGCTACC CAAGGAAGCC TGTATGGACA TTTCATCACC AGGGTGCCAG 
CCCCCTGCCC AGAGGCTGTG CCAGTCTGTT CCACCGAGCC CAGACTGCCA GCTGGTTCTA 
CACCAAGTGC TGAAAGGTGG CTCAGGGACA TATTGCCTCA ATGTGTCTTT GGCTGACGCC 
AACAGCCTGG CAGTGGCCAG CACCCAACTT GTTGTTCCTG GTCAAGACGG TGGCCTTGGG 
CAGGCTCCCT TGCTTGTAGG TATCTTGCTG GTGTTGGTGG CTGTGGTCCT TGCATCTCTG 
ATACATAGGC ATAGACTTAA GAAGCAGGGC TCAGTTTCCC AAATGCCACA TGGTAGCACT 
CACTGGCTGC GCCTGCCTCC GGTCTTCCGC GCCCGCGGCC TTGGAGAAAA CAGCCCGCTC 
CTCAGTGGAC AGCAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006513468.1

Translation

MGVQRRSFLPVLVLSALLAVGALEGSRNQDWLGVPRQLVTKTWNRQLYPEWTEVQGSNCWRGGQVSLRVINDGPTLVGANASFSIALHFPGSQKVLPDGQVIWANNTIINGSQVWGGQPVYPQEPDDACVFPDGGPCPSGPKPPKRSFVYVWKTWGKYWQVLGGPVSRLSIATGHAKLGTHTMEVTVYHRRGSQSYVPLAHASSTFTITDQVPFSVSVSQLQALDGETKHFLRNHPLIFALQLHDPSGYLAEADLSYTWDFGDGTGTLISRALDVTHTYLESGSVTAQVVLQAAIPLVSCGSSPVPGTTDGYMPTAEAPGTTSRQGTTTKVVGTTPGQMPTTQPSGTTVVQMPTTEVTATTSEQMLTSAVIDTTLAEVSTTEGTGTTPTRPSGTTVAQATTTEGPDASPLLPTQSSTGSISPLLDDTDTIMLVKRQVPLDCVLYRYGSFSLALDIVQGIESAEILQAVPFSEGDAFELTVSCQGGLPKEACMDISSPGCQPPAQRLCQSVPPSPDCQLVLHQVLKGGSGTYCLNVSLADANSLAVASTQLVVPGQDGGLGQAPLLVGILLVLVAVVLASLIHRHRLKKQGSVSQMPHGSTHWLRLPPVFRARGLGENSPLLSGQQV

Target ORF information:

RefSeq Version	XM_006513405.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus premelanosome protein (Pmel), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513405.2

ORF Insert Sequence:

ATGGGTGTCC AGAGAAGGAG CTTCCTTCCC GTGCTTGTGC TGAGTGCTCT GCTGGCTGTG 
GGGGCCCTAG AAGGATCCAG GAATCAGGAC TGGCTTGGTG TCCCAAGACA ACTTGTAACT 
AAAACCTGGA ACAGGCAGCT GTACCCCGAG TGGACAGAGG TGCAGGGGTC TAACTGCTGG 
AGAGGTGGCC AGGTATCTCT GAGGGTCATT AATGATGGGC CTACACTGGT TGGTGCAAAT 
GCCTCCTTTT CCATTGCCCT GCACTTCCCT GGAAGTCAAA AGGTACTACC GGATGGTCAG 
GTTATCTGGG CCAACAACAC CATCATCAAT GGGAGCCAGG TGTGGGGAGG ACAGCCAGTG 
TATCCACAGG AGCCTGATGA TGCCTGTGTC TTCCCTGACG GTGGACCCTG CCCATCTGGT 
CCTAAACCTC CGAAGAGAAG CTTTGTTTAT GTTTGGAAGA CCTGGGGAAA ATACTGGCAA 
GTTCTGGGGG GTCCAGTGTC CAGGCTGAGC ATTGCTACGG GCCACGCAAA GCTGGGCACA 
CACACAATGG AAGTGACTGT CTACCACCGA CGGGGTTCCC AGAGCTACGT GCCCCTTGCT 
CACGCCAGTT CAACCTTCAC CATTACTGAC CAGGTACCTT TCTCCGTGAG TGTGTCCCAG 
CTACAGGCCT TGGACGGAGA GACCAAGCAC TTCCTGAGAA ATCATCCTCT CATCTTTGCC 
CTTCAGCTCC ACGACCCCAG TGGTTATTTG GCCGAGGCCG ACCTCTCCTA CACATGGGAC 
TTTGGAGATG GTACTGGGAC CCTGATCTCT CGGGCACTTG ATGTCACTCA CACTTACCTG 
GAGTCGGGCT CAGTCACTGC CCAGGTGGTA CTGCAGGCTG CCATTCCTCT TGTTTCCTGT 
GGTTCCTCCC CAGTCCCGGG TACCACAGAT GGCTACATGC CAACTGCAGA AGCACCTGGA 
ACCACATCTA GGCAAGGAAC CACTACAAAA GTTGTGGGTA CTACACCTGG CCAGATGCCA 
ACTACACAGC CCTCTGGAAC CACAGTTGTA CAAATGCCAA CCACAGAGGT CACAGCTACT 
ACATCTGAGC AGATGCTGAC CTCAGCGGTC ATAGATACCA CACTGGCAGA GGTGTCAACT 
ACAGAGGGTA CAGGTACCAC ACCCACAAGG CCTTCTGGAA CCACCGTTGC ACAAGCAACA 
ACCACAGAGG GTCCAGATGC CAGCCCATTG CTGCCCACAC AAAGTTCTAC AGGGTCCATT 
AGCCCTCTAC TGGATGACAC CGACACCATA ATGCTTGTGA AGAGACAAGT TCCCCTGGAC 
TGTGTTCTAT ATCGATATGG TTCTTTCTCC CTCGCCCTGG ACATTGTCCA GGGTATTGAA 
AGTGCTGAGA TCCTGCAGGC TGTGCCATTC AGTGAAGGGG ATGCATTTGA GCTGACTGTG 
TCCTGCCAAG GCGGGCTACC CAAGGAAGCC TGTATGGACA TTTCATCACC AGGGTGCCAG 
CCCCCTGCCC AGAGGCTGTG CCAGTCTGTT CCACCGAGCC CAGACTGCCA GCTGGTTCTA 
CACCAAGTGC TGAAAGGTGG CTCAGGGACA TATTGCCTCA ATGTGTCTTT GGCTGACGCC 
AACAGCCTGG CAGTGGCCAG CACCCAACTT GTTGTTCCTG GTCAAGACGG TGGCCTTGGG 
CAGGCTCCCT TGCTTGTAGG TATCTTGCTG GTGTTGGTGG CTGTGGTCCT TGCATCTCTG 
ATACATAGGC ATAGACTTAA GAAGCAGGGC TCAGTTTCCC AAATGCCACA TGGTAGCACT 
CACTGGCTGC GCCTGCCTCC GGTCTTCCGC GCCCGCGGCC TTGGAGAAAA CAGCCCGCTC 
CTCAGTGGAC AGCAGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of microRNA-136 on melanoma cell proliferation, apoptosis, and epithelial-mesenchymal transition by targetting PMEL through the Wnt signaling pathway.
Bioscience reports37(5)(2017 Oct)
Wang JJ,Li ZF,Li XJ,Han Z,Zhang L,Liu ZJ

The Histone Methyltransferase Ezh2 Controls Mechanisms of Adaptive Resistance to Tumor Immunotherapy.
Cell reports20(4)854-867(2017 Jul)
Zingg D,Arenas-Ramirez N,Sahin D,Rosalia RA,Antunes AT,Haeusel J,Sommer L,Boyman O

DAPL1, a susceptibility locus for age-related macular degeneration, acts as a novel suppressor of cell proliferation in the retinal pigment epithelium.
Human molecular genetics26(9)1612-1621(2017 05)
Ma X,Li H,Wang Y,Wang J,Zheng Q,Hua J,Yang J,Pan L,Lu F,Qu J,Hou L

TFAP2 paralogs regulate melanocyte differentiation in parallel with MITF.
PLoS genetics13(3)e1006636(2017 Mar)
Seberg HE,Van Otterloo E,Loftus SK,Liu H,Bonde G,Sompallae R,Gildea DE,Santana JF,Manak JR,Pavan WJ,Williams T,Cornell RA

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PMEL cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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