Glg1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Glg1
Entrez Gene ID	20340
Full Name	golgi apparatus protein 1
Synonyms	AI593353,AW537898,CFR,CFR-1,ESL-1,MG-160,MG160,Selel
General protein information	Preferred Names golgi apparatus protein 1 Names Golgi apparatus protein 1 E-selectin ligand 1 golgi sialoglycoprotein MG-160 selectin, endothelial cell, ligand
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 E1\|8 57.98 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001368315.1	NP_001355244.1	Golgi apparatus protein 1 isoform 1 precursor
NM_009149.2	NP_033175.1	Golgi apparatus protein 1 precursor
NM_009149.3	NP_033175.1	Golgi apparatus protein 1 isoform 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLG1	NP_036333.2
Canis lupus familiaris (dog)	GLG1	XP_536786.2
Bos taurus (cattle)	GLG1	NP_001231069.1
Rattus norvegicus (Norway rat)	Glg1	NP_058907.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001320	XP_321825.4
Pan troglodytes (chimpanzee)	GLG1	XP_511104.3
Mus musculus (house mouse)	Glg1	NP_033175.1
Danio rerio (zebrafish)	glg1a	XP_005163270.1
Gallus gallus (chicken)	GLG1	NP_990827.1
	GLG1	XP_002802614.1
Caenorhabditis elegans (roundworm)	CELE_F14E5.2	NP_001022088.1
Xenopus tropicalis (tropical clawed frog)	glg1	XP_004914112.1
Drosophila melanogaster (fruit fly)	Glg1	NP_001262163.1

Gene Ontology
Bibliography

Related articles in PubMed

Polysialylation and lipopolysaccharide-induced shedding of E-selectin ligand-1 and neuropilin-2 by microglia and THP-1 macrophages.
Werneburg S, Buettner FF, Erben L, Mathews M, Neumann H, M?hlenhoff M, Hildebrandt H
Glia64(8)1314-30(2016 Aug)

Haematopoietic ESL-1 enables stem cell proliferation in the bone marrow by limiting TGF? availability.
Leiva M, Quintana JA, Ligos JM, Hidalgo A
Nature communications710222(2016 Jan)

Extracellular MRP8/14 is a regulator of ?2 integrin-dependent neutrophil slow rolling and adhesion.
Pruenster M, Kurz AR, Chung KJ, Cao-Ehlker X, Bieber S, Nussbaum CF, Bierschenk S, Eggersmann TK, Rohwedder I, Heinig K, Immler R, Moser M, Koedel U, Gran S, McEver RP, Vestweber D, Verschoor A, Leanderson T, Chavakis T, Roth J, Vogl T, Sperandio M
Nature communications66915(2015 Apr)

Coordinated and unique functions of the E-selectin ligand ESL-1 during inflammatory and hematopoietic recruitment in mice.
Sreeramkumar V, Leiva M, Stadtmann A, Pitaval C, Ortega-Rodr?guez I, Wild MK, Lee B, Zarbock A, Hidalgo A
Blood122(24)3993-4001(2013 Dec)

E-selectin ligand 1 regulates bone remodeling by limiting bioactive TGF-? in the bone microenvironment.
Yang T, Grafe I, Bae Y, Chen S, Chen Y, Bertin TK, Jiang MM, Ambrose CG, Lee B
Proceedings of the National Academy of Sciences of the United States of America110(18)7336-41(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identifies NRP2 and ESL-1 as targets for polysialylation in murine microglia and human THP-1 macrophages and reveals a striking convergence in the regulation of the two polysialylated acceptors in the course of inflammatory activation. A pool of polysialylated NRP2 and ESL-1 is assembled after injury or in culture and its shedding is an early response to lipopolysaccharide-induced activation of microglia.
Title: Polysialylation and lipopolysaccharide-induced shedding of E-selectin ligand-1 and neuropilin-2 by microglia and THP-1 macrophages.

A cell-intrinsic mechanism whereby haematopoietic cells limit proliferation within the bone marrow is repressed by E-selectin ligand 1 (ESL-1).
Title: Haematopoietic ESL-1 enables stem cell proliferation in the bone marrow by limiting TGF? availability.

Data indicate that only the combined deficiency in PSGL-1 and ESL-1 completely abrogated leukocyte recruitment.
Title: Coordinated and unique functions of the E-selectin ligand ESL-1 during inflammatory and hematopoietic recruitment in mice.

striking differences between the requirement of FucT-VII and C2GlcNAcT-I for Ligands for E-selectin and P-selectin expression in CD4+ T cells.
Title: Differential regulation and impact of fucosyltransferase VII and core 2 ?1,6-N-acetyl-glycosaminyltransferase for generation of E-selectin and P-selectin ligands in murine CD4+ T cells.

Heterozygous deficiency of ESL-1 is associated with features of increased atherosclerotic plaque stability while complete deficiency of ESL-1 leads to embryonic lethality.
Title: Haploinsufficiency of E-selectin ligand-1 is associated with reduced atherosclerotic plaque macrophage content while complete deficiency leads to early embryonic lethality in mice.

The following Glg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Glg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84470	NM_001368315.1 Latest version!	Mus musculus golgi apparatus protein 1 (Glg1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu03611	NM_009149.3 Latest version!	Mus musculus golgi apparatus protein 1 (Glg1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$692.30 $989.00
View Old Accession Versions >>
OMu03611	NM_009149.2	Mus musculus golgi apparatus protein 1 (Glg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$692.30 $989.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Glg1 ( NM_001368315.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001355244.1 Mus musculus golgi apparatus protein 1 (Glg1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu84470
Clone ID Related Accession (Same CDS sequence)	NM_001368315.1
Accession Version	NM_001368315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Mus musculus(house mouse)
Product	Golgi apparatus protein 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163625.5 and AC093451.25. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.225073.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGTGT GTGGACGTGT ACGGGGGATG TTCCGCTTGT CGGCGGCGTT GCCGCTGCTG 
CTGCTCGCGG CAGCAGGGGC CCAGAATGGT CACGGTCAGG GCCAGGGTCC GGGGACCAAC 
TTTGGGCCCT TCCCAGGGCA AGGTGGAGGC GGCAGCCCGG CCGGCCAACA GCCGCCTCAG 
CAGCCTCAGT TATCGCAGCA GCAGCAGCAG CCGCCGCCTC AGCAGCAGCA GCAGCAACAG 
CAGCAGTCGC TTTTCGCGGC GGGCGGGCTC CCGGCCCGGC GGGGCGGAGC GGGGCCCGGT 
GGGACTGGCG GAGGCTGGAA GCTGGCGGAG GAAGAGTCCT GCCGGGAGGA CGTGACCCGT 
GTGTGCCCCA AACACACCTG GAGCAACAAC CTGGCGGTGC TCGAGTGCCT GCAGGACGTG 
AGGGAGCCCG AGAACGAGAT TTCTTCAGAC TGCAATCATT TGTTGTGGAA TTACAAGCTG 
AACCTGACTA CTGATCCCAA ATTTGAATCT GTGGCCAGGG AGGTTTGCAA GTCAACTATT 
TCCGAGATTA AAGAATGTGC GGAAGAACCA GTTGGAAAAG GTTACATGGT TTCCTGCTTG 
GTGGATCATC GAGGCAACAT CACTGAGTAT CAGTGTCATC AATACATTAC CAAGATGACG 
GCCATCATTT TCAGTGATTA TCGCTTGATC TGCGGCTTCA TGGATGACTG CAAAAATGAT 
ATCAACCTCT TGAAATGTGG CAGCATTCGT CTTGGGGAAA AGGATGCACA TTCACAAGGT 
GAGGTGGTAT CATGTTTGGA AAAAGGCCTG GTAAAAGAGG CAGAAGAAAA AGAGCCGAAG 
ATTCAAGTCT CAGAACTCTG CAAGAAAGCC ATTCTTAGGG TGGCTGAGCT GTCCTCGGAT 
GACTTTCATT TAGACCGGCA TTTGTATTTT GCTTGCCGAG ATGATCGGGA GCGCTTTTGC 
GAGAATACAC AAGCTGGTGA AGGAAGAGTG TATAAATGCC TATTTAACCA TAAGTTTGAA 
GAATCCATGA GTGAAAAGTG TAGAGAAGCA CTAACTACAC GCCAGAAGCT CATTGCCCAG 
GATTATAAAG TCAGTTACTC ATTAGCCAAA TCCTGTAAGA GTGACTTGAA AAAATACCGG 
TGCAATGTGG AAAACCTTCC TCGGTCCCGG GAAGCCAGGC TCTCCTACCT TCTGATGTGC 
CTGGAGTCAG CAGTGCACAG AGGGCGGCAG GTGAGCAGTG AGTGCCAAGG TGAGATGCTG 
GATTACCGAC GCATGCTGAT GGAAGACTTC TCTCTGAGCC CCGAGATCAT CCTGAGCTGT 
CGAGGGGAGA TTGAACACCA TTGTTCTGGA TTACATCGGA AAGGGCGAAC CCTCCACTGT 
CTGATGAAAG TGGTTCGGGG TGAAAAGGGG AACCTTGGAA TGAACTGCCA ACAGGCGCTT 
CAGACACTGA TTCAGGAGAC TGACCCTGGT GCAGACTACC GCATTGATCG AGCTTTGAAT 
GAAGCTTGTG AGTCTGTAAT ACAGACAGCC TGCAAACACA TACGATCCGG AGACCCAATG 
ATTCTCTCAT GTCTGATGGA GCATTTATAC ACAGAGAAGA TGGTGGAAGA CTGTGAACAC 
CGGCTCTTAG AGCTACAGTA TTTTATCTCT CGGGATTGGA AGTTGGACCC TGTTTTATAC 
CGAAAATGCC AGGGAGATGC TTCCCGCCTT TGCCATACCC ATGGTTGGAA TGAGACCAGT 
GAACTTATGC CCCCTGGAGC TGTGTTTTCT TGCCTATACA GACATGCCTA CCGCACAGAA 
GAGCAAGGAA GGAGGCTCTC ACGAGAATGT CGAGCTGAAG TCCAGAGGAT CCTGCACCAG 
CGAGCCATGG ATGTTAAGCT GGATCCTGCC CTCCAGGACA AGTGCCTCAT AGACCTAGGG 
AAGTGGTGCA GTGAGAAGAC GGAGACTGGG CAGGAGCTTG AGTGCCTTCA GGACCATCTC 
GATGACTTAG CTGTGGAATG CAGAGACATC GTGGGCAACC TCACCGAGTT AGAGTCCGAG 
GATATACAAA TAGAAGCTTT GCTGATGAGA GCCTGTGAGC CTATCATTCA GAACTTTTGC 
CACGATGTGG CAGACAACCA GATCGACTCT GGGGACCTGA TGGAGTGTCT GATCCAGAAC 
AAGCACCAGA AGGACATGAA CGAGAAGTGT GCCATTGGCG TCACTCACTT CCAGCTGGTA 
CAGATGAAGG ATTTTCGATT CTCTTACAAG TTCAAAATGG CCTGCAAGGA GGATGTGTTA 
AAGCTTTGCC CCAACATAAA AAAGAAGGTG GACGTAGTGA TCTGCCTGAG CACCACTGTG 
CGCAACGACA CTCTGCAGGA GGCCAAGGAG CACCGAGTAT CACTCAAGTG CCGCAAGCAG 
CTTCGTGTGG AAGAGCTGGA GATGACAGAG GACATCCGTT TGGAACCAGA TCTGTATGAA 
GCCTGCAAGA GTGACATCAA GAACTACTGT TCTACAGTGC AATATGGAAA TGCTCAGATT 
ATTGAATGTC TAAAAGAAAA CAAGAAGCAG CTGAGTACCC GTTGCCACCA GAAAGTATTT 
AAGCTGCAGG AGACGGAGAT GATGGACCCA GAGCTAGACT ATACTCTGAT GAGAGTCTGC 
AAGCAGATGA TTAAGCGGTT CTGTCCAGAA GCAGATTCTA AAACTATGTT GCAGTGTTTA 
AAACAAAATA AGAACAGTGA ATTGATGGAT CCCAAATGCA AACAGATGAT AACCAAGCGC 
CAGATCACCC AGAACACAGA TTACCGCTTA AACCCTGTGC TAAGGAAGGC CTGTAAAGCT 
GACATTCCTA AGTTCTGTCA TGGCATCCTG ACCAAGGCTA AGGACGATTC AGAGCTAGAA 
GGCCAAGTTA TCTCATGCCT CAAGCTGAGA TATGCTGACC AGCGCCTGTC CTCAGACTGT 
GAGGACCAGA TCCGTATCAT CATCCAGGAG TCTGCTCTGG ATTACCGCCT GGACCCTCAG 
CTCCAGCTGC ACTGCTCAGA TGAGATTGCC AATCTATGTG CTGAAGAAGC AGCAGCCCAG 
GAGCAAACAG GCCAAGTGGA AGAGTGCCTG AAGGTCAACC TGCTCAAGAT CAAGACAGAG 
CTGTGTAAAA AGAGTATTAC AACAACAGAT TGGATACCTT TGTTCTACAA AGCTCTCTCC 
ACAATGAACT TCCCTTTTCA GGAAGTGTTA AACATGTTGA AGGAAAGCAA AGCAGACATC 
TTTGTGGACC CCGTTCTTCA CACAGCATGT GCCTTGGACA TTAAACACCA CTGTGCAGCC 
ATCACCCCTG GCCGCGGGCG TCAGATGTCC TGCCTGATGG AGGCCCTGGA AGATAAGCGA 
GTGAGATTGC AGCCAGAGTG CAAAAAGCGC CTCAATGACC GGATTGAGAT GTGGAGTTAT 
GCAGCCAAGG TGGCTCCAGC AGATGGCTTC TCTGATCTTG CCATGCAAGT GATGACATCT 
CCCTCAAAGA ACTACATCCT GTCTGTGATC AGCGGGAGCA TCTGCATACT CTTCCTGATT 
GGCCTGATGT GTGGACGGAT CACCAAGAGA GTGACACGAG AGCTGAAGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355244.1
CDS	15..3611
Translation	MAVCGRVRGMFRLSAALPLLLLAAAGAQNGHGQGQGPGTNFGPFPGQGGGGSPAGQQPPQQPQLSQQQQQPPPQQQQQQQQQSLFAAGGLPARRGGAGPGGTGGGWKLAEEESCREDVTRVCPKHTWSNNLAVLECLQDVREPENEISSDCNHLLWNYKLNLTTDPKFESVAREVCKSTISEIKECAEEPVGKGYMVSCLVDHRGNITEYQCHQYITKMTAIIFSDYRLICGFMDDCKNDINLLKCGSIRLGEKDAHSQGEVVSCLEKGLVKEAEEKEPKIQVSELCKKAILRVAELSSDDFHLDRHLYFACRDDRERFCENTQAGEGRVYKCLFNHKFEESMSEKCREALTTRQKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRSREARLSYLLMCLESAVHRGRQVSSECQGEMLDYRRMLMEDFSLSPEIILSCRGEIEHHCSGLHRKGRTLHCLMKVVRGEKGNLGMNCQQALQTLIQETDPGADYRIDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLDPVLYRKCQGDASRLCHTHGWNETSELMPPGAVFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRAMDVKLDPALQDKCLIDLGKWCSEKTETGQELECLQDHLDDLAVECRDIVGNLTELESEDIQIEALLMRACEPIIQNFCHDVADNQIDSGDLMECLIQNKHQKDMNEKCAIGVTHFQLVQMKDFRFSYKFKMACKEDVLKLCPNIKKKVDVVICLSTTVRNDTLQEAKEHRVSLKCRKQLRVEELEMTEDIRLEPDLYEACKSDIKNYCSTVQYGNAQIIECLKENKKQLSTRCHQKVFKLQETEMMDPELDYTLMRVCKQMIKRFCPEADSKTMLQCLKQNKNSELMDPKCKQMITKRQITQNTDYRLNPVLRKACKADIPKFCHGILTKAKDDSELEGQVISCLKLRYADQRLSSDCEDQIRIIIQESALDYRLDPQLQLHCSDEIANLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKSITTTDWIPLFYKALSTMNFPFQEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNYILSVISGSICILFLIGLMCGRITKRVTRELKDR

Target ORF information:

RefSeq Version	NM_001368315.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus golgi apparatus protein 1 (Glg1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368315.1

ORF Insert Sequence:

ATGGCGGTGT GTGGACGTGT ACGGGGGATG TTCCGCTTGT CGGCGGCGTT GCCGCTGCTG 
CTGCTCGCGG CAGCAGGGGC CCAGAATGGT CACGGTCAGG GCCAGGGTCC GGGGACCAAC 
TTTGGGCCCT TCCCAGGGCA AGGTGGAGGC GGCAGCCCGG CCGGCCAACA GCCGCCTCAG 
CAGCCTCAGT TATCGCAGCA GCAGCAGCAG CCGCCGCCTC AGCAGCAGCA GCAGCAACAG 
CAGCAGTCGC TTTTCGCGGC GGGCGGGCTC CCGGCCCGGC GGGGCGGAGC GGGGCCCGGT 
GGGACTGGCG GAGGCTGGAA GCTGGCGGAG GAAGAGTCCT GCCGGGAGGA CGTGACCCGT 
GTGTGCCCCA AACACACCTG GAGCAACAAC CTGGCGGTGC TCGAGTGCCT GCAGGACGTG 
AGGGAGCCCG AGAACGAGAT TTCTTCAGAC TGCAATCATT TGTTGTGGAA TTACAAGCTG 
AACCTGACTA CTGATCCCAA ATTTGAATCT GTGGCCAGGG AGGTTTGCAA GTCAACTATT 
TCCGAGATTA AAGAATGTGC GGAAGAACCA GTTGGAAAAG GTTACATGGT TTCCTGCTTG 
GTGGATCATC GAGGCAACAT CACTGAGTAT CAGTGTCATC AATACATTAC CAAGATGACG 
GCCATCATTT TCAGTGATTA TCGCTTGATC TGCGGCTTCA TGGATGACTG CAAAAATGAT 
ATCAACCTCT TGAAATGTGG CAGCATTCGT CTTGGGGAAA AGGATGCACA TTCACAAGGT 
GAGGTGGTAT CATGTTTGGA AAAAGGCCTG GTAAAAGAGG CAGAAGAAAA AGAGCCGAAG 
ATTCAAGTCT CAGAACTCTG CAAGAAAGCC ATTCTTAGGG TGGCTGAGCT GTCCTCGGAT 
GACTTTCATT TAGACCGGCA TTTGTATTTT GCTTGCCGAG ATGATCGGGA GCGCTTTTGC 
GAGAATACAC AAGCTGGTGA AGGAAGAGTG TATAAATGCC TATTTAACCA TAAGTTTGAA 
GAATCCATGA GTGAAAAGTG TAGAGAAGCA CTAACTACAC GCCAGAAGCT CATTGCCCAG 
GATTATAAAG TCAGTTACTC ATTAGCCAAA TCCTGTAAGA GTGACTTGAA AAAATACCGG 
TGCAATGTGG AAAACCTTCC TCGGTCCCGG GAAGCCAGGC TCTCCTACCT TCTGATGTGC 
CTGGAGTCAG CAGTGCACAG AGGGCGGCAG GTGAGCAGTG AGTGCCAAGG TGAGATGCTG 
GATTACCGAC GCATGCTGAT GGAAGACTTC TCTCTGAGCC CCGAGATCAT CCTGAGCTGT 
CGAGGGGAGA TTGAACACCA TTGTTCTGGA TTACATCGGA AAGGGCGAAC CCTCCACTGT 
CTGATGAAAG TGGTTCGGGG TGAAAAGGGG AACCTTGGAA TGAACTGCCA ACAGGCGCTT 
CAGACACTGA TTCAGGAGAC TGACCCTGGT GCAGACTACC GCATTGATCG AGCTTTGAAT 
GAAGCTTGTG AGTCTGTAAT ACAGACAGCC TGCAAACACA TACGATCCGG AGACCCAATG 
ATTCTCTCAT GTCTGATGGA GCATTTATAC ACAGAGAAGA TGGTGGAAGA CTGTGAACAC 
CGGCTCTTAG AGCTACAGTA TTTTATCTCT CGGGATTGGA AGTTGGACCC TGTTTTATAC 
CGAAAATGCC AGGGAGATGC TTCCCGCCTT TGCCATACCC ATGGTTGGAA TGAGACCAGT 
GAACTTATGC CCCCTGGAGC TGTGTTTTCT TGCCTATACA GACATGCCTA CCGCACAGAA 
GAGCAAGGAA GGAGGCTCTC ACGAGAATGT CGAGCTGAAG TCCAGAGGAT CCTGCACCAG 
CGAGCCATGG ATGTTAAGCT GGATCCTGCC CTCCAGGACA AGTGCCTCAT AGACCTAGGG 
AAGTGGTGCA GTGAGAAGAC GGAGACTGGG CAGGAGCTTG AGTGCCTTCA GGACCATCTC 
GATGACTTAG CTGTGGAATG CAGAGACATC GTGGGCAACC TCACCGAGTT AGAGTCCGAG 
GATATACAAA TAGAAGCTTT GCTGATGAGA GCCTGTGAGC CTATCATTCA GAACTTTTGC 
CACGATGTGG CAGACAACCA GATCGACTCT GGGGACCTGA TGGAGTGTCT GATCCAGAAC 
AAGCACCAGA AGGACATGAA CGAGAAGTGT GCCATTGGCG TCACTCACTT CCAGCTGGTA 
CAGATGAAGG ATTTTCGATT CTCTTACAAG TTCAAAATGG CCTGCAAGGA GGATGTGTTA 
AAGCTTTGCC CCAACATAAA AAAGAAGGTG GACGTAGTGA TCTGCCTGAG CACCACTGTG 
CGCAACGACA CTCTGCAGGA GGCCAAGGAG CACCGAGTAT CACTCAAGTG CCGCAAGCAG 
CTTCGTGTGG AAGAGCTGGA GATGACAGAG GACATCCGTT TGGAACCAGA TCTGTATGAA 
GCCTGCAAGA GTGACATCAA GAACTACTGT TCTACAGTGC AATATGGAAA TGCTCAGATT 
ATTGAATGTC TAAAAGAAAA CAAGAAGCAG CTGAGTACCC GTTGCCACCA GAAAGTATTT 
AAGCTGCAGG AGACGGAGAT GATGGACCCA GAGCTAGACT ATACTCTGAT GAGAGTCTGC 
AAGCAGATGA TTAAGCGGTT CTGTCCAGAA GCAGATTCTA AAACTATGTT GCAGTGTTTA 
AAACAAAATA AGAACAGTGA ATTGATGGAT CCCAAATGCA AACAGATGAT AACCAAGCGC 
CAGATCACCC AGAACACAGA TTACCGCTTA AACCCTGTGC TAAGGAAGGC CTGTAAAGCT 
GACATTCCTA AGTTCTGTCA TGGCATCCTG ACCAAGGCTA AGGACGATTC AGAGCTAGAA 
GGCCAAGTTA TCTCATGCCT CAAGCTGAGA TATGCTGACC AGCGCCTGTC CTCAGACTGT 
GAGGACCAGA TCCGTATCAT CATCCAGGAG TCTGCTCTGG ATTACCGCCT GGACCCTCAG 
CTCCAGCTGC ACTGCTCAGA TGAGATTGCC AATCTATGTG CTGAAGAAGC AGCAGCCCAG 
GAGCAAACAG GCCAAGTGGA AGAGTGCCTG AAGGTCAACC TGCTCAAGAT CAAGACAGAG 
CTGTGTAAAA AGAGTATTAC AACAACAGAT TGGATACCTT TGTTCTACAA AGCTCTCTCC 
ACAATGAACT TCCCTTTTCA GGAAGTGTTA AACATGTTGA AGGAAAGCAA AGCAGACATC 
TTTGTGGACC CCGTTCTTCA CACAGCATGT GCCTTGGACA TTAAACACCA CTGTGCAGCC 
ATCACCCCTG GCCGCGGGCG TCAGATGTCC TGCCTGATGG AGGCCCTGGA AGATAAGCGA 
GTGAGATTGC AGCCAGAGTG CAAAAAGCGC CTCAATGACC GGATTGAGAT GTGGAGTTAT 
GCAGCCAAGG TGGCTCCAGC AGATGGCTTC TCTGATCTTG CCATGCAAGT GATGACATCT 
CCCTCAAAGA ACTACATCCT GTCTGTGATC AGCGGGAGCA TCTGCATACT CTTCCTGATT 
GGCCTGATGT GTGGACGGAT CACCAAGAGA GTGACACGAG AGCTGAAGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Glg1 ( NM_009149.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033175.1 Mus musculus golgi apparatus protein 1 (Glg1), mRNA.

Price & Availability↑

CloneID	OMu03611
Clone ID Related Accession (Same CDS sequence)	NM_009149.2 , NM_009149.3
Accession Version	NM_009149.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-10
Organism	Mus musculus(house mouse)
Product	Golgi apparatus protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC163625.5 and AC093451.25. On Nov 17, 2006 this sequence version replaced NM_009149.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X84037.1, AK154581.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGTGT GTGGACGTGT ACGGGGGATG TTCCGCTTGT CGGCGGCGTT GCCGCTGCTG 
CTGCTCGCGG CAGCAGGGGC CCAGAATGGT CACGGTCAGG GCCAGGGTCC GGGGACCAAC 
TTTGGGCCCT TCCCAGGGCA AGGTGGAGGC GGCAGCCCGG CCGGCCAACA GCCGCCTCAG 
CAGCCTCAGT TATCGCAGCA GCAGCAGCAG CCGCCGCCTC AGCAGCAGCA GCAGCAACAG 
CAGCAGTCGC TTTTCGCGGC GGGCGGGCTC CCGGCCCGGC GGGGCGGAGC GGGGCCCGGT 
GGGACTGGCG GAGGCTGGAA GCTGGCGGAG GAAGAGTCCT GCCGGGAGGA CGTGACCCGT 
GTGTGCCCCA AACACACCTG GAGCAACAAC CTGGCGGTGC TCGAGTGCCT GCAGGACGTG 
AGGGAGCCCG AGAACGAGAT TTCTTCAGAC TGCAATCATT TGTTGTGGAA TTACAAGCTG 
AACCTGACTA CTGATCCCAA ATTTGAATCT GTGGCCAGGG AGGTTTGCAA GTCAACTATT 
TCCGAGATTA AAGAATGTGC GGAAGAACCA GTTGGAAAAG GTTACATGGT TTCCTGCTTG 
GTGGATCATC GAGGCAACAT CACTGAGTAT CAGTGTCATC AATACATTAC CAAGATGACG 
GCCATCATTT TCAGTGATTA TCGCTTGATC TGCGGCTTCA TGGATGACTG CAAAAATGAT 
ATCAACCTCT TGAAATGTGG CAGCATTCGT CTTGGGGAAA AGGATGCACA TTCACAAGGT 
GAGGTGGTAT CATGTTTGGA AAAAGGCCTG GTAAAAGAGG CAGAAGAAAA AGAGCCGAAG 
ATTCAAGTCT CAGAACTCTG CAAGAAAGCC ATTCTTAGGG TGGCTGAGCT GTCCTCGGAT 
GACTTTCATT TAGACCGGCA TTTGTATTTT GCTTGCCGAG ATGATCGGGA GCGCTTTTGC 
GAGAATACAC AAGCTGGTGA AGGAAGAGTG TATAAATGCC TATTTAACCA TAAGTTTGAA 
GAATCCATGA GTGAAAAGTG TAGAGAAGCA CTAACTACAC GCCAGAAGCT CATTGCCCAG 
GATTATAAAG TCAGTTACTC ATTAGCCAAA TCCTGTAAGA GTGACTTGAA AAAATACCGG 
TGCAATGTGG AAAACCTTCC TCGGTCCCGG GAAGCCAGGC TCTCCTACCT TCTGATGTGC 
CTGGAGTCAG CAGTGCACAG AGGGCGGCAG GTGAGCAGTG AGTGCCAAGG TGAGATGCTG 
GATTACCGAC GCATGCTGAT GGAAGACTTC TCTCTGAGCC CCGAGATCAT CCTGAGCTGT 
CGAGGGGAGA TTGAACACCA TTGTTCTGGA TTACATCGGA AAGGGCGAAC CCTCCACTGT 
CTGATGAAAG TGGTTCGGGG TGAAAAGGGG AACCTTGGAA TGAACTGCCA ACAGGCGCTT 
CAGACACTGA TTCAGGAGAC TGACCCTGGT GCAGACTACC GCATTGATCG AGCTTTGAAT 
GAAGCTTGTG AGTCTGTAAT ACAGACAGCC TGCAAACACA TACGATCCGG AGACCCAATG 
ATTCTCTCAT GTCTGATGGA GCATTTATAC ACAGAGAAGA TGGTGGAAGA CTGTGAACAC 
CGGCTCTTAG AGCTACAGTA TTTTATCTCT CGGGATTGGA AGTTGGACCC TGTTTTATAC 
CGAAAATGCC AGGGAGATGC TTCCCGCCTT TGCCATACCC ATGGTTGGAA TGAGACCAGT 
GAACTTATGC CCCCTGGAGC TGTGTTTTCT TGCCTATACA GACATGCCTA CCGCACAGAA 
GAGCAAGGAA GGAGGCTCTC ACGAGAATGT CGAGCTGAAG TCCAGAGGAT CCTGCACCAG 
CGAGCCATGG ATGTTAAGCT GGATCCTGCC CTCCAGGACA AGTGCCTCAT AGACCTAGGG 
AAGTGGTGCA GTGAGAAGAC GGAGACTGGG CAGGAGCTTG AGTGCCTTCA GGACCATCTC 
GATGACTTAG CTGTGGAATG CAGAGACATC GTGGGCAACC TCACCGAGTT AGAGTCCGAG 
GATATACAAA TAGAAGCTTT GCTGATGAGA GCCTGTGAGC CTATCATTCA GAACTTTTGC 
CACGATGTGG CAGACAACCA GATCGACTCT GGGGACCTGA TGGAGTGTCT GATCCAGAAC 
AAGCACCAGA AGGACATGAA CGAGAAGTGT GCCATTGGCG TCACTCACTT CCAGCTGGTA 
CAGATGAAGG ATTTTCGATT CTCTTACAAG TTCAAAATGG CCTGCAAGGA GGATGTGTTA 
AAGCTTTGCC CCAACATAAA AAAGAAGGTG GACGTAGTGA TCTGCCTGAG CACCACTGTG 
CGCAACGACA CTCTGCAGGA GGCCAAGGAG CACCGAGTAT CACTCAAGTG CCGCAAGCAG 
CTTCGTGTGG AAGAGCTGGA GATGACAGAG GACATCCGTT TGGAACCAGA TCTGTATGAA 
GCCTGCAAGA GTGACATCAA GAACTACTGT TCTACAGTGC AATATGGAAA TGCTCAGATT 
ATTGAATGTC TAAAAGAAAA CAAGAAGCAG CTGAGTACCC GTTGCCACCA GAAAGTATTT 
AAGCTGCAGG AGACGGAGAT GATGGACCCA GAGCTAGACT ATACTCTGAT GAGAGTCTGC 
AAGCAGATGA TTAAGCGGTT CTGTCCAGAA GCAGATTCTA AAACTATGTT GCAGTGTTTA 
AAACAAAATA AGAACAGTGA ATTGATGGAT CCCAAATGCA AACAGATGAT AACCAAGCGC 
CAGATCACCC AGAACACAGA TTACCGCTTA AACCCTGTGC TAAGGAAGGC CTGTAAAGCT 
GACATTCCTA AGTTCTGTCA TGGCATCCTG ACCAAGGCTA AGGACGATTC AGAGCTAGAA 
GGCCAAGTTA TCTCATGCCT CAAGCTGAGA TATGCTGACC AGCGCCTGTC CTCAGACTGT 
GAGGACCAGA TCCGTATCAT CATCCAGGAG TCTGCTCTGG ATTACCGCCT GGACCCTCAG 
CTCCAGCTGC ACTGCTCAGA TGAGATTGCC AATCTATGTG CTGAAGAAGC AGCAGCCCAG 
GAGCAAACAG GCCAAGTGGA AGAGTGCCTG AAGGTCAACC TGCTCAAGAT CAAGACAGAG 
CTGTGTAAAA AGGAAGTGTT AAACATGTTG AAGGAAAGCA AAGCAGACAT CTTTGTGGAC 
CCCGTTCTTC ACACAGCATG TGCCTTGGAC ATTAAACACC ACTGTGCAGC CATCACCCCT 
GGCCGCGGGC GTCAGATGTC CTGCCTGATG GAGGCCCTGG AAGATAAGCG AGTGAGATTG 
CAGCCAGAGT GCAAAAAGCG CCTCAATGAC CGGATTGAGA TGTGGAGTTA TGCAGCCAAG 
GTGGCTCCAG CAGATGGCTT CTCTGATCTT GCCATGCAAG TGATGACATC TCCCTCAAAG 
AACTACATCC TGTCTGTGAT CAGCGGGAGC ATCTGCATAC TCTTCCTGAT TGGCCTGATG 
TGTGGACGGA TCACCAAGAG AGTGACACGA GAGCTGAAGG ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033175.1
CDS	7..3534
Translation	MAVCGRVRGMFRLSAALPLLLLAAAGAQNGHGQGQGPGTNFGPFPGQGGGGSPAGQQPPQQPQLSQQQQQPPPQQQQQQQQQSLFAAGGLPARRGGAGPGGTGGGWKLAEEESCREDVTRVCPKHTWSNNLAVLECLQDVREPENEISSDCNHLLWNYKLNLTTDPKFESVAREVCKSTISEIKECAEEPVGKGYMVSCLVDHRGNITEYQCHQYITKMTAIIFSDYRLICGFMDDCKNDINLLKCGSIRLGEKDAHSQGEVVSCLEKGLVKEAEEKEPKIQVSELCKKAILRVAELSSDDFHLDRHLYFACRDDRERFCENTQAGEGRVYKCLFNHKFEESMSEKCREALTTRQKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRSREARLSYLLMCLESAVHRGRQVSSECQGEMLDYRRMLMEDFSLSPEIILSCRGEIEHHCSGLHRKGRTLHCLMKVVRGEKGNLGMNCQQALQTLIQETDPGADYRIDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLDPVLYRKCQGDASRLCHTHGWNETSELMPPGAVFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRAMDVKLDPALQDKCLIDLGKWCSEKTETGQELECLQDHLDDLAVECRDIVGNLTELESEDIQIEALLMRACEPIIQNFCHDVADNQIDSGDLMECLIQNKHQKDMNEKCAIGVTHFQLVQMKDFRFSYKFKMACKEDVLKLCPNIKKKVDVVICLSTTVRNDTLQEAKEHRVSLKCRKQLRVEELEMTEDIRLEPDLYEACKSDIKNYCSTVQYGNAQIIECLKENKKQLSTRCHQKVFKLQETEMMDPELDYTLMRVCKQMIKRFCPEADSKTMLQCLKQNKNSELMDPKCKQMITKRQITQNTDYRLNPVLRKACKADIPKFCHGILTKAKDDSELEGQVISCLKLRYADQRLSSDCEDQIRIIIQESALDYRLDPQLQLHCSDEIANLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNYILSVISGSICILFLIGLMCGRITKRVTRELKDR

Target ORF information:

RefSeq Version	NM_009149.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus golgi apparatus protein 1 (Glg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009149.2

ORF Insert Sequence:

ATGGCGGTGT GTGGACGTGT ACGGGGGATG TTCCGCTTGT CGGCGGCGTT GCCGCTGCTG 
CTGCTCGCGG CAGCAGGGGC CCAGAATGGT CACGGTCAGG GCCAGGGTCC GGGGACCAAC 
TTTGGGCCCT TCCCAGGGCA AGGTGGAGGC GGCAGCCCGG CCGGCCAACA GCCGCCTCAG 
CAGCCTCAGT TATCGCAGCA GCAGCAGCAG CCGCCGCCTC AGCAGCAGCA GCAGCAACAG 
CAGCAGTCGC TTTTCGCGGC GGGCGGGCTC CCGGCCCGGC GGGGCGGAGC GGGGCCCGGT 
GGGACTGGCG GAGGCTGGAA GCTGGCGGAG GAAGAGTCCT GCCGGGAGGA CGTGACCCGT 
GTGTGCCCCA AACACACCTG GAGCAACAAC CTGGCGGTGC TCGAGTGCCT GCAGGACGTG 
AGGGAGCCCG AGAACGAGAT TTCTTCAGAC TGCAATCATT TGTTGTGGAA TTACAAGCTG 
AACCTGACTA CTGATCCCAA ATTTGAATCT GTGGCCAGGG AGGTTTGCAA GTCAACTATT 
TCCGAGATTA AAGAATGTGC GGAAGAACCA GTTGGAAAAG GTTACATGGT TTCCTGCTTG 
GTGGATCATC GAGGCAACAT CACTGAGTAT CAGTGTCATC AATACATTAC CAAGATGACG 
GCCATCATTT TCAGTGATTA TCGCTTGATC TGCGGCTTCA TGGATGACTG CAAAAATGAT 
ATCAACCTCT TGAAATGTGG CAGCATTCGT CTTGGGGAAA AGGATGCACA TTCACAAGGT 
GAGGTGGTAT CATGTTTGGA AAAAGGCCTG GTAAAAGAGG CAGAAGAAAA AGAGCCGAAG 
ATTCAAGTCT CAGAACTCTG CAAGAAAGCC ATTCTTAGGG TGGCTGAGCT GTCCTCGGAT 
GACTTTCATT TAGACCGGCA TTTGTATTTT GCTTGCCGAG ATGATCGGGA GCGCTTTTGC 
GAGAATACAC AAGCTGGTGA AGGAAGAGTG TATAAATGCC TATTTAACCA TAAGTTTGAA 
GAATCCATGA GTGAAAAGTG TAGAGAAGCA CTAACTACAC GCCAGAAGCT CATTGCCCAG 
GATTATAAAG TCAGTTACTC ATTAGCCAAA TCCTGTAAGA GTGACTTGAA AAAATACCGG 
TGCAATGTGG AAAACCTTCC TCGGTCCCGG GAAGCCAGGC TCTCCTACCT TCTGATGTGC 
CTGGAGTCAG CAGTGCACAG AGGGCGGCAG GTGAGCAGTG AGTGCCAAGG TGAGATGCTG 
GATTACCGAC GCATGCTGAT GGAAGACTTC TCTCTGAGCC CCGAGATCAT CCTGAGCTGT 
CGAGGGGAGA TTGAACACCA TTGTTCTGGA TTACATCGGA AAGGGCGAAC CCTCCACTGT 
CTGATGAAAG TGGTTCGGGG TGAAAAGGGG AACCTTGGAA TGAACTGCCA ACAGGCGCTT 
CAGACACTGA TTCAGGAGAC TGACCCTGGT GCAGACTACC GCATTGATCG AGCTTTGAAT 
GAAGCTTGTG AGTCTGTAAT ACAGACAGCC TGCAAACACA TACGATCCGG AGACCCAATG 
ATTCTCTCAT GTCTGATGGA GCATTTATAC ACAGAGAAGA TGGTGGAAGA CTGTGAACAC 
CGGCTCTTAG AGCTACAGTA TTTTATCTCT CGGGATTGGA AGTTGGACCC TGTTTTATAC 
CGAAAATGCC AGGGAGATGC TTCCCGCCTT TGCCATACCC ATGGTTGGAA TGAGACCAGT 
GAACTTATGC CCCCTGGAGC TGTGTTTTCT TGCCTATACA GACATGCCTA CCGCACAGAA 
GAGCAAGGAA GGAGGCTCTC ACGAGAATGT CGAGCTGAAG TCCAGAGGAT CCTGCACCAG 
CGAGCCATGG ATGTTAAGCT GGATCCTGCC CTCCAGGACA AGTGCCTCAT AGACCTAGGG 
AAGTGGTGCA GTGAGAAGAC GGAGACTGGG CAGGAGCTTG AGTGCCTTCA GGACCATCTC 
GATGACTTAG CTGTGGAATG CAGAGACATC GTGGGCAACC TCACCGAGTT AGAGTCCGAG 
GATATACAAA TAGAAGCTTT GCTGATGAGA GCCTGTGAGC CTATCATTCA GAACTTTTGC 
CACGATGTGG CAGACAACCA GATCGACTCT GGGGACCTGA TGGAGTGTCT GATCCAGAAC 
AAGCACCAGA AGGACATGAA CGAGAAGTGT GCCATTGGCG TCACTCACTT CCAGCTGGTA 
CAGATGAAGG ATTTTCGATT CTCTTACAAG TTCAAAATGG CCTGCAAGGA GGATGTGTTA 
AAGCTTTGCC CCAACATAAA AAAGAAGGTG GACGTAGTGA TCTGCCTGAG CACCACTGTG 
CGCAACGACA CTCTGCAGGA GGCCAAGGAG CACCGAGTAT CACTCAAGTG CCGCAAGCAG 
CTTCGTGTGG AAGAGCTGGA GATGACAGAG GACATCCGTT TGGAACCAGA TCTGTATGAA 
GCCTGCAAGA GTGACATCAA GAACTACTGT TCTACAGTGC AATATGGAAA TGCTCAGATT 
ATTGAATGTC TAAAAGAAAA CAAGAAGCAG CTGAGTACCC GTTGCCACCA GAAAGTATTT 
AAGCTGCAGG AGACGGAGAT GATGGACCCA GAGCTAGACT ATACTCTGAT GAGAGTCTGC 
AAGCAGATGA TTAAGCGGTT CTGTCCAGAA GCAGATTCTA AAACTATGTT GCAGTGTTTA 
AAACAAAATA AGAACAGTGA ATTGATGGAT CCCAAATGCA AACAGATGAT AACCAAGCGC 
CAGATCACCC AGAACACAGA TTACCGCTTA AACCCTGTGC TAAGGAAGGC CTGTAAAGCT 
GACATTCCTA AGTTCTGTCA TGGCATCCTG ACCAAGGCTA AGGACGATTC AGAGCTAGAA 
GGCCAAGTTA TCTCATGCCT CAAGCTGAGA TATGCTGACC AGCGCCTGTC CTCAGACTGT 
GAGGACCAGA TCCGTATCAT CATCCAGGAG TCTGCTCTGG ATTACCGCCT GGACCCTCAG 
CTCCAGCTGC ACTGCTCAGA TGAGATTGCC AATCTATGTG CTGAAGAAGC AGCAGCCCAG 
GAGCAAACAG GCCAAGTGGA AGAGTGCCTG AAGGTCAACC TGCTCAAGAT CAAGACAGAG 
CTGTGTAAAA AGGAAGTGTT AAACATGTTG AAGGAAAGCA AAGCAGACAT CTTTGTGGAC 
CCCGTTCTTC ACACAGCATG TGCCTTGGAC ATTAAACACC ACTGTGCAGC CATCACCCCT 
GGCCGCGGGC GTCAGATGTC CTGCCTGATG GAGGCCCTGG AAGATAAGCG AGTGAGATTG 
CAGCCAGAGT GCAAAAAGCG CCTCAATGAC CGGATTGAGA TGTGGAGTTA TGCAGCCAAG 
GTGGCTCCAG CAGATGGCTT CTCTGATCTT GCCATGCAAG TGATGACATC TCCCTCAAAG 
AACTACATCC TGTCTGTGAT CAGCGGGAGC ATCTGCATAC TCTTCCTGAT TGGCCTGATG 
TGTGGACGGA TCACCAAGAG AGTGACACGA GAGCTGAAGG ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Glg1 ( NM_009149.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033175.1 Mus musculus golgi apparatus protein 1 (Glg1), transcript variant 2, mRNA.

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CloneID	OMu03611
Clone ID Related Accession (Same CDS sequence)	NM_009149.2 , NM_009149.3
Accession Version	NM_009149.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Mus musculus(house mouse)
Product	Golgi apparatus protein 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163625.5 and AC093451.25. On Feb 6, 2019 this sequence version replaced NM_009149.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X84037.1, AK154581.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGTGT GTGGACGTGT ACGGGGGATG TTCCGCTTGT CGGCGGCGTT GCCGCTGCTG 
CTGCTCGCGG CAGCAGGGGC CCAGAATGGT CACGGTCAGG GCCAGGGTCC GGGGACCAAC 
TTTGGGCCCT TCCCAGGGCA AGGTGGAGGC GGCAGCCCGG CCGGCCAACA GCCGCCTCAG 
CAGCCTCAGT TATCGCAGCA GCAGCAGCAG CCGCCGCCTC AGCAGCAGCA GCAGCAACAG 
CAGCAGTCGC TTTTCGCGGC GGGCGGGCTC CCGGCCCGGC GGGGCGGAGC GGGGCCCGGT 
GGGACTGGCG GAGGCTGGAA GCTGGCGGAG GAAGAGTCCT GCCGGGAGGA CGTGACCCGT 
GTGTGCCCCA AACACACCTG GAGCAACAAC CTGGCGGTGC TCGAGTGCCT GCAGGACGTG 
AGGGAGCCCG AGAACGAGAT TTCTTCAGAC TGCAATCATT TGTTGTGGAA TTACAAGCTG 
AACCTGACTA CTGATCCCAA ATTTGAATCT GTGGCCAGGG AGGTTTGCAA GTCAACTATT 
TCCGAGATTA AAGAATGTGC GGAAGAACCA GTTGGAAAAG GTTACATGGT TTCCTGCTTG 
GTGGATCATC GAGGCAACAT CACTGAGTAT CAGTGTCATC AATACATTAC CAAGATGACG 
GCCATCATTT TCAGTGATTA TCGCTTGATC TGCGGCTTCA TGGATGACTG CAAAAATGAT 
ATCAACCTCT TGAAATGTGG CAGCATTCGT CTTGGGGAAA AGGATGCACA TTCACAAGGT 
GAGGTGGTAT CATGTTTGGA AAAAGGCCTG GTAAAAGAGG CAGAAGAAAA AGAGCCGAAG 
ATTCAAGTCT CAGAACTCTG CAAGAAAGCC ATTCTTAGGG TGGCTGAGCT GTCCTCGGAT 
GACTTTCATT TAGACCGGCA TTTGTATTTT GCTTGCCGAG ATGATCGGGA GCGCTTTTGC 
GAGAATACAC AAGCTGGTGA AGGAAGAGTG TATAAATGCC TATTTAACCA TAAGTTTGAA 
GAATCCATGA GTGAAAAGTG TAGAGAAGCA CTAACTACAC GCCAGAAGCT CATTGCCCAG 
GATTATAAAG TCAGTTACTC ATTAGCCAAA TCCTGTAAGA GTGACTTGAA AAAATACCGG 
TGCAATGTGG AAAACCTTCC TCGGTCCCGG GAAGCCAGGC TCTCCTACCT TCTGATGTGC 
CTGGAGTCAG CAGTGCACAG AGGGCGGCAG GTGAGCAGTG AGTGCCAAGG TGAGATGCTG 
GATTACCGAC GCATGCTGAT GGAAGACTTC TCTCTGAGCC CCGAGATCAT CCTGAGCTGT 
CGAGGGGAGA TTGAACACCA TTGTTCTGGA TTACATCGGA AAGGGCGAAC CCTCCACTGT 
CTGATGAAAG TGGTTCGGGG TGAAAAGGGG AACCTTGGAA TGAACTGCCA ACAGGCGCTT 
CAGACACTGA TTCAGGAGAC TGACCCTGGT GCAGACTACC GCATTGATCG AGCTTTGAAT 
GAAGCTTGTG AGTCTGTAAT ACAGACAGCC TGCAAACACA TACGATCCGG AGACCCAATG 
ATTCTCTCAT GTCTGATGGA GCATTTATAC ACAGAGAAGA TGGTGGAAGA CTGTGAACAC 
CGGCTCTTAG AGCTACAGTA TTTTATCTCT CGGGATTGGA AGTTGGACCC TGTTTTATAC 
CGAAAATGCC AGGGAGATGC TTCCCGCCTT TGCCATACCC ATGGTTGGAA TGAGACCAGT 
GAACTTATGC CCCCTGGAGC TGTGTTTTCT TGCCTATACA GACATGCCTA CCGCACAGAA 
GAGCAAGGAA GGAGGCTCTC ACGAGAATGT CGAGCTGAAG TCCAGAGGAT CCTGCACCAG 
CGAGCCATGG ATGTTAAGCT GGATCCTGCC CTCCAGGACA AGTGCCTCAT AGACCTAGGG 
AAGTGGTGCA GTGAGAAGAC GGAGACTGGG CAGGAGCTTG AGTGCCTTCA GGACCATCTC 
GATGACTTAG CTGTGGAATG CAGAGACATC GTGGGCAACC TCACCGAGTT AGAGTCCGAG 
GATATACAAA TAGAAGCTTT GCTGATGAGA GCCTGTGAGC CTATCATTCA GAACTTTTGC 
CACGATGTGG CAGACAACCA GATCGACTCT GGGGACCTGA TGGAGTGTCT GATCCAGAAC 
AAGCACCAGA AGGACATGAA CGAGAAGTGT GCCATTGGCG TCACTCACTT CCAGCTGGTA 
CAGATGAAGG ATTTTCGATT CTCTTACAAG TTCAAAATGG CCTGCAAGGA GGATGTGTTA 
AAGCTTTGCC CCAACATAAA AAAGAAGGTG GACGTAGTGA TCTGCCTGAG CACCACTGTG 
CGCAACGACA CTCTGCAGGA GGCCAAGGAG CACCGAGTAT CACTCAAGTG CCGCAAGCAG 
CTTCGTGTGG AAGAGCTGGA GATGACAGAG GACATCCGTT TGGAACCAGA TCTGTATGAA 
GCCTGCAAGA GTGACATCAA GAACTACTGT TCTACAGTGC AATATGGAAA TGCTCAGATT 
ATTGAATGTC TAAAAGAAAA CAAGAAGCAG CTGAGTACCC GTTGCCACCA GAAAGTATTT 
AAGCTGCAGG AGACGGAGAT GATGGACCCA GAGCTAGACT ATACTCTGAT GAGAGTCTGC 
AAGCAGATGA TTAAGCGGTT CTGTCCAGAA GCAGATTCTA AAACTATGTT GCAGTGTTTA 
AAACAAAATA AGAACAGTGA ATTGATGGAT CCCAAATGCA AACAGATGAT AACCAAGCGC 
CAGATCACCC AGAACACAGA TTACCGCTTA AACCCTGTGC TAAGGAAGGC CTGTAAAGCT 
GACATTCCTA AGTTCTGTCA TGGCATCCTG ACCAAGGCTA AGGACGATTC AGAGCTAGAA 
GGCCAAGTTA TCTCATGCCT CAAGCTGAGA TATGCTGACC AGCGCCTGTC CTCAGACTGT 
GAGGACCAGA TCCGTATCAT CATCCAGGAG TCTGCTCTGG ATTACCGCCT GGACCCTCAG 
CTCCAGCTGC ACTGCTCAGA TGAGATTGCC AATCTATGTG CTGAAGAAGC AGCAGCCCAG 
GAGCAAACAG GCCAAGTGGA AGAGTGCCTG AAGGTCAACC TGCTCAAGAT CAAGACAGAG 
CTGTGTAAAA AGGAAGTGTT AAACATGTTG AAGGAAAGCA AAGCAGACAT CTTTGTGGAC 
CCCGTTCTTC ACACAGCATG TGCCTTGGAC ATTAAACACC ACTGTGCAGC CATCACCCCT 
GGCCGCGGGC GTCAGATGTC CTGCCTGATG GAGGCCCTGG AAGATAAGCG AGTGAGATTG 
CAGCCAGAGT GCAAAAAGCG CCTCAATGAC CGGATTGAGA TGTGGAGTTA TGCAGCCAAG 
GTGGCTCCAG CAGATGGCTT CTCTGATCTT GCCATGCAAG TGATGACATC TCCCTCAAAG 
AACTACATCC TGTCTGTGAT CAGCGGGAGC ATCTGCATAC TCTTCCTGAT TGGCCTGATG 
TGTGGACGGA TCACCAAGAG AGTGACACGA GAGCTGAAGG ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033175.1
CDS	15..3542
Translation	MAVCGRVRGMFRLSAALPLLLLAAAGAQNGHGQGQGPGTNFGPFPGQGGGGSPAGQQPPQQPQLSQQQQQPPPQQQQQQQQQSLFAAGGLPARRGGAGPGGTGGGWKLAEEESCREDVTRVCPKHTWSNNLAVLECLQDVREPENEISSDCNHLLWNYKLNLTTDPKFESVAREVCKSTISEIKECAEEPVGKGYMVSCLVDHRGNITEYQCHQYITKMTAIIFSDYRLICGFMDDCKNDINLLKCGSIRLGEKDAHSQGEVVSCLEKGLVKEAEEKEPKIQVSELCKKAILRVAELSSDDFHLDRHLYFACRDDRERFCENTQAGEGRVYKCLFNHKFEESMSEKCREALTTRQKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRSREARLSYLLMCLESAVHRGRQVSSECQGEMLDYRRMLMEDFSLSPEIILSCRGEIEHHCSGLHRKGRTLHCLMKVVRGEKGNLGMNCQQALQTLIQETDPGADYRIDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLDPVLYRKCQGDASRLCHTHGWNETSELMPPGAVFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRAMDVKLDPALQDKCLIDLGKWCSEKTETGQELECLQDHLDDLAVECRDIVGNLTELESEDIQIEALLMRACEPIIQNFCHDVADNQIDSGDLMECLIQNKHQKDMNEKCAIGVTHFQLVQMKDFRFSYKFKMACKEDVLKLCPNIKKKVDVVICLSTTVRNDTLQEAKEHRVSLKCRKQLRVEELEMTEDIRLEPDLYEACKSDIKNYCSTVQYGNAQIIECLKENKKQLSTRCHQKVFKLQETEMMDPELDYTLMRVCKQMIKRFCPEADSKTMLQCLKQNKNSELMDPKCKQMITKRQITQNTDYRLNPVLRKACKADIPKFCHGILTKAKDDSELEGQVISCLKLRYADQRLSSDCEDQIRIIIQESALDYRLDPQLQLHCSDEIANLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNYILSVISGSICILFLIGLMCGRITKRVTRELKDR

Target ORF information:

RefSeq Version	NM_009149.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus golgi apparatus protein 1 (Glg1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009149.3

ORF Insert Sequence:

ATGGCGGTGT GTGGACGTGT ACGGGGGATG TTCCGCTTGT CGGCGGCGTT GCCGCTGCTG 
CTGCTCGCGG CAGCAGGGGC CCAGAATGGT CACGGTCAGG GCCAGGGTCC GGGGACCAAC 
TTTGGGCCCT TCCCAGGGCA AGGTGGAGGC GGCAGCCCGG CCGGCCAACA GCCGCCTCAG 
CAGCCTCAGT TATCGCAGCA GCAGCAGCAG CCGCCGCCTC AGCAGCAGCA GCAGCAACAG 
CAGCAGTCGC TTTTCGCGGC GGGCGGGCTC CCGGCCCGGC GGGGCGGAGC GGGGCCCGGT 
GGGACTGGCG GAGGCTGGAA GCTGGCGGAG GAAGAGTCCT GCCGGGAGGA CGTGACCCGT 
GTGTGCCCCA AACACACCTG GAGCAACAAC CTGGCGGTGC TCGAGTGCCT GCAGGACGTG 
AGGGAGCCCG AGAACGAGAT TTCTTCAGAC TGCAATCATT TGTTGTGGAA TTACAAGCTG 
AACCTGACTA CTGATCCCAA ATTTGAATCT GTGGCCAGGG AGGTTTGCAA GTCAACTATT 
TCCGAGATTA AAGAATGTGC GGAAGAACCA GTTGGAAAAG GTTACATGGT TTCCTGCTTG 
GTGGATCATC GAGGCAACAT CACTGAGTAT CAGTGTCATC AATACATTAC CAAGATGACG 
GCCATCATTT TCAGTGATTA TCGCTTGATC TGCGGCTTCA TGGATGACTG CAAAAATGAT 
ATCAACCTCT TGAAATGTGG CAGCATTCGT CTTGGGGAAA AGGATGCACA TTCACAAGGT 
GAGGTGGTAT CATGTTTGGA AAAAGGCCTG GTAAAAGAGG CAGAAGAAAA AGAGCCGAAG 
ATTCAAGTCT CAGAACTCTG CAAGAAAGCC ATTCTTAGGG TGGCTGAGCT GTCCTCGGAT 
GACTTTCATT TAGACCGGCA TTTGTATTTT GCTTGCCGAG ATGATCGGGA GCGCTTTTGC 
GAGAATACAC AAGCTGGTGA AGGAAGAGTG TATAAATGCC TATTTAACCA TAAGTTTGAA 
GAATCCATGA GTGAAAAGTG TAGAGAAGCA CTAACTACAC GCCAGAAGCT CATTGCCCAG 
GATTATAAAG TCAGTTACTC ATTAGCCAAA TCCTGTAAGA GTGACTTGAA AAAATACCGG 
TGCAATGTGG AAAACCTTCC TCGGTCCCGG GAAGCCAGGC TCTCCTACCT TCTGATGTGC 
CTGGAGTCAG CAGTGCACAG AGGGCGGCAG GTGAGCAGTG AGTGCCAAGG TGAGATGCTG 
GATTACCGAC GCATGCTGAT GGAAGACTTC TCTCTGAGCC CCGAGATCAT CCTGAGCTGT 
CGAGGGGAGA TTGAACACCA TTGTTCTGGA TTACATCGGA AAGGGCGAAC CCTCCACTGT 
CTGATGAAAG TGGTTCGGGG TGAAAAGGGG AACCTTGGAA TGAACTGCCA ACAGGCGCTT 
CAGACACTGA TTCAGGAGAC TGACCCTGGT GCAGACTACC GCATTGATCG AGCTTTGAAT 
GAAGCTTGTG AGTCTGTAAT ACAGACAGCC TGCAAACACA TACGATCCGG AGACCCAATG 
ATTCTCTCAT GTCTGATGGA GCATTTATAC ACAGAGAAGA TGGTGGAAGA CTGTGAACAC 
CGGCTCTTAG AGCTACAGTA TTTTATCTCT CGGGATTGGA AGTTGGACCC TGTTTTATAC 
CGAAAATGCC AGGGAGATGC TTCCCGCCTT TGCCATACCC ATGGTTGGAA TGAGACCAGT 
GAACTTATGC CCCCTGGAGC TGTGTTTTCT TGCCTATACA GACATGCCTA CCGCACAGAA 
GAGCAAGGAA GGAGGCTCTC ACGAGAATGT CGAGCTGAAG TCCAGAGGAT CCTGCACCAG 
CGAGCCATGG ATGTTAAGCT GGATCCTGCC CTCCAGGACA AGTGCCTCAT AGACCTAGGG 
AAGTGGTGCA GTGAGAAGAC GGAGACTGGG CAGGAGCTTG AGTGCCTTCA GGACCATCTC 
GATGACTTAG CTGTGGAATG CAGAGACATC GTGGGCAACC TCACCGAGTT AGAGTCCGAG 
GATATACAAA TAGAAGCTTT GCTGATGAGA GCCTGTGAGC CTATCATTCA GAACTTTTGC 
CACGATGTGG CAGACAACCA GATCGACTCT GGGGACCTGA TGGAGTGTCT GATCCAGAAC 
AAGCACCAGA AGGACATGAA CGAGAAGTGT GCCATTGGCG TCACTCACTT CCAGCTGGTA 
CAGATGAAGG ATTTTCGATT CTCTTACAAG TTCAAAATGG CCTGCAAGGA GGATGTGTTA 
AAGCTTTGCC CCAACATAAA AAAGAAGGTG GACGTAGTGA TCTGCCTGAG CACCACTGTG 
CGCAACGACA CTCTGCAGGA GGCCAAGGAG CACCGAGTAT CACTCAAGTG CCGCAAGCAG 
CTTCGTGTGG AAGAGCTGGA GATGACAGAG GACATCCGTT TGGAACCAGA TCTGTATGAA 
GCCTGCAAGA GTGACATCAA GAACTACTGT TCTACAGTGC AATATGGAAA TGCTCAGATT 
ATTGAATGTC TAAAAGAAAA CAAGAAGCAG CTGAGTACCC GTTGCCACCA GAAAGTATTT 
AAGCTGCAGG AGACGGAGAT GATGGACCCA GAGCTAGACT ATACTCTGAT GAGAGTCTGC 
AAGCAGATGA TTAAGCGGTT CTGTCCAGAA GCAGATTCTA AAACTATGTT GCAGTGTTTA 
AAACAAAATA AGAACAGTGA ATTGATGGAT CCCAAATGCA AACAGATGAT AACCAAGCGC 
CAGATCACCC AGAACACAGA TTACCGCTTA AACCCTGTGC TAAGGAAGGC CTGTAAAGCT 
GACATTCCTA AGTTCTGTCA TGGCATCCTG ACCAAGGCTA AGGACGATTC AGAGCTAGAA 
GGCCAAGTTA TCTCATGCCT CAAGCTGAGA TATGCTGACC AGCGCCTGTC CTCAGACTGT 
GAGGACCAGA TCCGTATCAT CATCCAGGAG TCTGCTCTGG ATTACCGCCT GGACCCTCAG 
CTCCAGCTGC ACTGCTCAGA TGAGATTGCC AATCTATGTG CTGAAGAAGC AGCAGCCCAG 
GAGCAAACAG GCCAAGTGGA AGAGTGCCTG AAGGTCAACC TGCTCAAGAT CAAGACAGAG 
CTGTGTAAAA AGGAAGTGTT AAACATGTTG AAGGAAAGCA AAGCAGACAT CTTTGTGGAC 
CCCGTTCTTC ACACAGCATG TGCCTTGGAC ATTAAACACC ACTGTGCAGC CATCACCCCT 
GGCCGCGGGC GTCAGATGTC CTGCCTGATG GAGGCCCTGG AAGATAAGCG AGTGAGATTG 
CAGCCAGAGT GCAAAAAGCG CCTCAATGAC CGGATTGAGA TGTGGAGTTA TGCAGCCAAG 
GTGGCTCCAG CAGATGGCTT CTCTGATCTT GCCATGCAAG TGATGACATC TCCCTCAAAG 
AACTACATCC TGTCTGTGAT CAGCGGGAGC ATCTGCATAC TCTTCCTGAT TGGCCTGATG 
TGTGGACGGA TCACCAAGAG AGTGACACGA GAGCTGAAGG ACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polysialylation and lipopolysaccharide-induced shedding of E-selectin ligand-1 and neuropilin-2 by microglia and THP-1 macrophages.
Glia64(8)1314-30(2016 Aug)
Werneburg S,Buettner FF,Erben L,Mathews M,Neumann H,M?hlenhoff M,Hildebrandt H

Haematopoietic ESL-1 enables stem cell proliferation in the bone marrow by limiting TGF? availability.
Nature communications710222(2016 Jan)
Leiva M,Quintana JA,Ligos JM,Hidalgo A

Extracellular MRP8/14 is a regulator of ?2 integrin-dependent neutrophil slow rolling and adhesion.
Nature communications66915(2015 Apr)
Pruenster M,Kurz AR,Chung KJ,Cao-Ehlker X,Bieber S,Nussbaum CF,Bierschenk S,Eggersmann TK,Rohwedder I,Heinig K,Immler R,Moser M,Koedel U,Gran S,McEver RP,Vestweber D,Verschoor A,Leanderson T,Chavakis T,Roth J,Vogl T,Sperandio M

Coordinated and unique functions of the E-selectin ligand ESL-1 during inflammatory and hematopoietic recruitment in mice.
Blood122(24)3993-4001(2013 Dec)
Sreeramkumar V,Leiva M,Stadtmann A,Pitaval C,Ortega-Rodr?guez I,Wild MK,Lee B,Zarbock A,Hidalgo A

E-selectin ligand 1 regulates bone remodeling by limiting bioactive TGF-? in the bone microenvironment.
Proceedings of the National Academy of Sciences of the United States of America110(18)7336-41(2013 Apr)
Yang T,Grafe I,Bae Y,Chen S,Chen Y,Bertin TK,Jiang MM,Ambrose CG,Lee B

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Glg1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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